CN103520368B - A kind of Traditional Chinese medicine health-preserving preparation with qi and blood tonifying, enhancing immunity function - Google Patents
A kind of Traditional Chinese medicine health-preserving preparation with qi and blood tonifying, enhancing immunity function Download PDFInfo
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Abstract
The present invention relates to a kind of Traditional Chinese medicine health-preserving preparation with qi and blood tonifying, enhancing immunity function.This Traditional Chinese medicine health-preserving preparation is made up of the crude drug of following part by weight: Colla Corii Asini 5 25, Radix Codonopsis 15 35, Radix Angelicae Sinensis 10 30, the Radix Astragali 10 30, Fructus Lycii 5 25, Cornu Cervi Pantotrichum 15, Heme iron element 15.Traditional Chinese medicine health-preserving preparation medicine assortment class is few, consumption proportion is precise and appropriate for this, there is mutual Synergistic function, the taste caused for insufficiency of vital energy and blood and weak, spiritlessness and weakness, hypoimmunity, lumbago abdominal distention, asthenia are sayed less, insomnia forgetfulness, shallow complexion, menoxenia, climacteric syndrome etc. have remarkable effect.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, particularly there is the Traditional Chinese medicine health-preserving preparation of qi and blood tonifying, enhancing immunity function.
Background technology
Traditional Chinese Medicine is thought, qi being the governor of blood, blood being the material foundation of QI, and QI and blood is the power and source of vital movement；Huangdi's Internal Classics is recorded: disharmony between QI and blood, and all kinds of diseases and ailments are to change and give birth to；QI and blood foot, all kinds of diseases and ailments are removed.
Chinese medicine is more about the report of qi and blood tonifying, enhancing immunity at present, and for different etiology and pathogenesis, dialectical difference, prescription is the most different.Patent CN91107958 (Publication No. CN1067816A) discloses a kind of Method of vital energy and blood tonifying medicine, and it makes dry cream after Chinese medicine astragalus, Radix Codonopsis, Radix Angelicae Sinensis, Fructus Lycii, Fructus Crataegi being extracted respectively；Take the dry cream of Fructus Lycii, Fructus Crataegi and Radix Angelicae Sinensis, add the adjuvants such as acidic flavoring agent and make granule A；Take Radix Codonopsis, the dry cream of the Radix Astragali mixes with Chinese medicine Colla Corii Asini, adds the adjuvants such as foaming agent and makes granule B；After bis-kinds of granules of A with B are mixed and get final product.Patent CN94103903 (Publication No. CN1097330A) discloses a kind of Resina Draconis oral liquid, and it contains following 10 kinds of original herbal: the Radix Astragali, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Herb Gynostemmae Pentaphylli, Radix Angelicae Sinensis, Fructus Lycii, Colla Corii Asini, Cornu Cervi Pantotrichum, Radix Glycyrrhizae, Fructus Jujubae.Patent CN98104770 (Publication No. CN1190589A) discloses a kind of complex ferrous sulfate tablet, it is made up of chalybeate, folic acid, yeast and Radix Angelicae Sinensis, also can add the Radix Astragali, and the arbitrary taste in the Rhizoma Atractylodis Macrocephalae, Pericarpium Citri Reticulatae, Radix Codonopsis, Fructus Jujubae, Colla Corii Asini, Radix Polygoni Multiflori, Fructus Lycii, Fructus Corni, Radix Rehmanniae Preparata, Radix Ginseng, Cornu Cervi Pantotrichum, Rhizoma Dioscoreae, vitamin C, Rhizoma Atractylodis can be added.Patent CN201010285377 (Publication No. CN101953997A) discloses a kind of YIN nourishing and YANG strengthening, the Chinese medicine of benefiting QI and nourishing blood, it is by Fructus Cnidii, Rhizoma Curculiginis, trachelospermum jasminoide, Rhizoma Cibotii, Tong puncture multitude, Radix Rehmanniae Preparata, Radix Ginseng, Rhizoma Polygonati, Herba Cistanches, Herba Orobanches, Radix Morindae Officinalis, Radix Polygalae, Radix Angelicae Sinensis, Fructus Psoraleae, Semen Cuscutae, Radix Adenophorae (Radix Glehniae), Fructus Schisandrae Chinensis, Fructus Rubi, Radix Asparagi, Radix Polygoni Multiflori, Fructus Ligustri Lucidi, Herba Dendrobii, the Radix Astragali, Radix Polygalae, male Bombycis mori, Aspongopus, the Cortex Eucommiae, Cornu Cervi Pantotrichum, Semen Celosiae, Semen Platycladi, Semen Allii Tuberosi, Rhizoma Acori Graminei, Herba Alii fistulosi, Arillus Longan, Semen Coicis, Herba Cynomorii, Fructus Akebiae, Semen Euryales, Fructus Lycii, Hippocampus, Testis et penis callorhini, Ootheca Mantidis, Solenognathus, Radix Ophiopogonis, Semen Plantaginis, Radix Achyranthis Bidentatae, Penis et testis cervi, the medicine compositions such as Semen Momordicae Charantiae.Patent CN201210215054 (Publication No. CN102813742A) discloses a kind of natural medicine-food homology raw material having and improving nutritional anemia, and it is made up of following raw material: the Radix Astragali, Radix Angelicae Sinensis, Radix Codonopsis, Fructus Lycii, Colla Corii Asini, white sugar, potassium sorbate, purified water.
But the most reasonably use Chinese medical theory, find the striving direction that medical material kind is few, consumption proportion is precise and appropriate, mutual Synergistic, prescription evident in efficacy are current work of Chinese medicine persons.
Colla Corii Asini: enrich blood；Hemostasis；YIN nourishing；Moisturize.Main syndrome of deficiency of blood；Asthenia is spat blood；Spit blood；Hematuria；Have blood in stool；Dysentery；Gestation hemorrhage；Metrorrhagia；The vexed insomnia of the deficiency of YIN；Deficiency of the lung bath is coughed；The convulsion of stirring-up of pathogenic wind in the interior resulting from deficiency is fainted tic.
Radix Codonopsis: invigorating middle warmer, QI invigorating, promote the production of body fluid." medicinal herbs among the people of science ": hematinic.It is applicable to chronic anaemia, chlorosis, leukemia, adenopathy, rickets.
Radix Angelicae Sinensis: replenishing and activating blood, menstruction regulating and pain relieving, loosening bowel to relieve constipation.For blood deficiency and yellow complexion, dizziness cardiopalmus, menoxenia, amenorrhea dysmenorrhea, asthenia cold abdominalgia, dryness of the intestine constipation, rheumatic arthralgia, injury from falling down, ulcer sores.
The Radix Astragali: Radix Astragali sweet in the mouth, feeble QI temperature, gas is thin and taste is dense, can rise and can drop, and the yang aspect of yang is also, nontoxic.Specially QI invigorating.Start with lunar, the warp of the few the moon of the most lunar, the hands of foot.Its function is a lot of, and its only effect person, especially enriching blood.
Fructus Lycii: the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity, blood-enriching tranquillizing, promoting the production of body fluid to quench thirst, nourishing the lung to arrest cough.Control hepatic and renal YIN deficiency, soreness of the waist and knees, dizzy, dizzy, the many tear of blurred vision, cough due to consumptive disease, quench one's thirst, seminal emission.
Cornu Cervi Pantotrichum: sweet, salty, warm.Return kidney, Liver Channel.Energy temperature compensation Liver and kidney, benefiting essence-blood, bone and muscle strengthening, for tonifying YANG Conclusion Decreasing, the key medicine of supplying vital essence and marrow, promote erythrocyte, haemachrome, reticulocyte and hematoblastic generation.
Ferrum is the indispensable composition of body, it is the component part constituting hemoglobin, Myoglobin, cyto-chromatin and histaminase (cytochrome enzyme, cytochrome oxidase, peroxidase, catalase) etc., carries oxygen so that they have and use oxygen function.Heme iron is directly absorbed by intestinal epithelial cell with the form of ferrous porphyrin, and absorbance is high, thus eliminates chemistry ferrum (such as ferrous sulfate) and stimulate toxicity and the intestines and stomach of human body, enables that ferrum is more efficient, is more safely absorbed by the body utilization.
Summary of the invention
For reaching object above, medical material kind is few, consumption proportion is precise and appropriate to provide one, there is mutual Synergistic, there is the Traditional Chinese medicine health-preserving preparation of qi and blood tonifying, enhancing immunity function, we are according to motherland's medical science understanding to QI and blood theory, base oneself upon the Chinese medicine basic principle by vigorate qi and replenish the blood, with reference to modern pharmacological research achievement and former various proved recipes, grain is gone to take essence, seven flavor medicine is eaten the raw material of homology, rigorous compatibility, is prepared as a kind of Traditional Chinese medicine health-preserving preparation with qi and blood tonifying, enhancing immunity function.Taste that said preparation causes for insufficiency of vital energy and blood and weak, spiritlessness and weakness, hypoimmunity, lumbago abdominal distention, asthenia are sayed less, insomnia forgetfulness, shallow complexion, menoxenia, climacteric syndrome etc. have remarkable effect.
This Traditional Chinese medicine health-preserving preparation is made up of the crude drug of following part by weight: Colla Corii Asini 5-25, Radix Codonopsis 15-35, Radix Angelicae Sinensis 10-30, Radix Astragali 10-30, Fructus Lycii 5-25, Cornu Cervi Pantotrichum 1-5, heme iron 1-5.
Preferably: Colla Corii Asini 10-20, Radix Codonopsis 20-30, Radix Angelicae Sinensis 15-25, Radix Astragali 15-25, Fructus Lycii 10-20, Cornu Cervi Pantotrichum 2-3, heme iron 3-4.
Further preferred: Colla Corii Asini 15, Radix Codonopsis 25, Radix Angelicae Sinensis 20, the Radix Astragali 20, Fructus Lycii 15, Cornu Cervi Pantotrichum 2, heme iron 3.
The preparation method of above-mentioned Traditional Chinese medicine health-preserving preparation comprises the following steps:
(1) weigh Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2-3 time by weight proportion, add the water of 8-10 times amount, 2-3 hour extraction time every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
Or further mixture powder mixed homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
Present invention also offers the application in the medicine preparing qi and blood tonifying, enhancing immunity function of the above-mentioned Traditional Chinese medicine health-preserving preparation.
Containing Colla Corii Asini, Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum and heme iron seven taste raw material in this Traditional Chinese medicine health-preserving preparation.Said preparation bases oneself upon the Chinese medicine basic theories to vigorate qi and replenish the blood, has reached the function of qi and blood tonifying, enhancing immunity.The flat sweet in the mouth of Colla Corii Asini, enters lung Liver and kidney warp, focuses on blood enriching and yin nourishing and moisturize；Radix Angelicae Sinensis acrid-sweet flavor warm in nature, enters liver heart spleen channel, has effect of replenishing and activating blood, loosening bowel to relieve constipation, and Radix Angelicae Sinensis invigorating middle warmer has, has benefit, the gas medicine in sincere blood, the also panacea in blood in row；Radix Codonopsis, the Radix Astragali focus on invigorating the spleen and replenishing QI, strengthening spleen and tonifying lung；Fructus Lycii, Cornu Cervi Pantotrichum focus on nourishing the liver and kidney, replenishing vital essence to improve eyesight, bone and muscle strengthening；Being used in combination of Colla Corii Asini, Radix Angelicae Sinensis and heme iron, strengthen blood nourishing function, and mend and oiliness；All medicine compatibilities, qi and blood tonifying, mend and oiliness, overall conditioning.Taste that this Traditional Chinese medicine health-preserving preparation causes for insufficiency of vital energy and blood and weak, spiritlessness and weakness, hypoimmunity, lumbago abdominal distention, asthenia are sayed less, insomnia forgetfulness, shallow complexion, menoxenia, climacteric syndrome etc. have remarkable effect.
Detailed description of the invention
Embodiment 1
Take crude drug: Colla Corii Asini 18g, Radix Codonopsis 25g, Radix Angelicae Sinensis 20g, Radix Astragali 20g, Fructus Lycii 15g, Cornu Cervi Pantotrichum 2g, heme iron 3g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2 times, add the water of 8 times amount, 2.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
Embodiment 2
Take crude drug: Colla Corii Asini 10g, Radix Codonopsis 20g, Radix Angelicae Sinensis 15g, Radix Astragali 25g, Fructus Lycii 20g, Cornu Cervi Pantotrichum 3g, heme iron 4g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 3 times, add the water of 10 times amount, 3.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained；
(4) mixture powder mixed homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
Embodiment 3
Take crude drug: Colla Corii Asini 20g, Radix Codonopsis 30g, Radix Angelicae Sinensis 25g, Radix Astragali 15g, Fructus Lycii 10g, Cornu Cervi Pantotrichum 2g, heme iron 3g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2 times, add the water of 10 times amount, 3.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
Embodiment 4
Take crude drug: Colla Corii Asini 5g, Radix Codonopsis 15g, Radix Angelicae Sinensis 10g, Radix Astragali 30g, Fructus Lycii 25g, Cornu Cervi Pantotrichum 5g, heme iron 5g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 3 times, add the water of 8 times amount, 3.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained；
(4) mixture powder mixed homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
Embodiment 5
Take crude drug: Colla Corii Asini 25g, Radix Codonopsis 35g, Radix Angelicae Sinensis 30g, Radix Astragali 10g, Fructus Lycii 5g, Cornu Cervi Pantotrichum 1g, heme iron 1g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2 times, add the water of 9 times amount, 2.5 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
Embodiment 6
Take crude drug: Colla Corii Asini 15g, Radix Codonopsis 25g, Radix Angelicae Sinensis 20g, Radix Astragali 20g, Fructus Lycii 15g, Cornu Cervi Pantotrichum 2g, heme iron 3g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 3 times, add the water of 9 times amount, 2.5 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained；
(4) mixture powder mixed homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
Embodiment 7
Take crude drug: Colla Corii Asini 10g, Radix Codonopsis 30g, Radix Angelicae Sinensis 15g, Radix Astragali 25g, Fructus Lycii 20g, Cornu Cervi Pantotrichum 3g, heme iron 4g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2 times, add the water of 8 times amount, 2.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
Embodiment 8
Take crude drug: Colla Corii Asini 20g, Radix Codonopsis 20g, Radix Angelicae Sinensis 25g, Radix Astragali 15g, Fructus Lycii 10g, Cornu Cervi Pantotrichum 2g, heme iron 3g.
Preparation method is:
(1) by above-mentioned weight weighing Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 3 times, add the water of 10 times amount, 3.0 hours extraction times every time, filter, merge twice filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then carry out that (vacuum is 0.06～0.08MPa) is concentrated in vacuo and obtain thick extractum to relative density 1.20～1.25 (60 DEG C measure)；Thick extractum carries out being vacuum dried and pulverizing and to obtain Chinese medicinal components extract powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained；
(4) mixture powder mixed homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
Test example 1 Report on Animal
Sample: the granule of embodiment 6 preparation.
Laboratory animal: 18-22g female SPF Kunming mouse, is provided by hygienic conditions Institute for Medical Research of Academy of Military Medicine, PLA.Raising temperature: 20-25 DEG C, humidity: 40-70%RH.
Dosage choice and animal subject give mode: the sample of the present invention sets 0.5,1.0, tri-dosage groups of 2.0g/kg BW (being respectively equivalent to 5 times of people's plan dosage, 10 times, 20 times, respectively low dose group, middle dosage group, high dose group)；Set the matched group (normal control 1 of most preferred embodiment in Basal control group, patent CN91107958 again, dosage is 2.0g/kg BW) and patent CN201210215054 in the matched group (normal control 2, dosage is 2.0g/kg BW) of embodiment one.Basic, normal, high dosage group and normal control group 1 take granule 5.0g, 10.0g, 20.0g and 20.0g respectively, all add distilled water to 200ml, fully mix, by 0.2ml/10g BW gavage；Normal control group 2 gives and the medicinal liquid of high dose group equivalent, and matched group gives equivalent distilled water, once a day, continuous 30 days, and last measured indices to after tested material 24 hours.Laboratory animal is divided into 4 big group (immunity 1-4 group), and often group 60, the most often 60 animals of group are divided into again 6 groups, respectively Basal control, normal control 1, normal control 2 and basic, normal, high three dosage groups, often group 10.Wherein the 1st big group carries out HC50Measure, antibody-producting cell detects, delayed allergy (the foot sole of the foot thickens method)；2nd big group carries out Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell test, dirty/body ratio measurement；3rd big group carries out carbonic clearance test；4th big group carries out mouse lymphocyte conversion test, the test of NK cytoactive detection.
Key instrument and reagent: cleaning stations, aseptic dissection equipment, RPMI1640 cell culture fluid, canavaline, isopropanol, MTT, slide gauge, SRBC, microsyringe, Dou Shi reagent, india ink, chicken red blood cell, LDH base fluid, timer, hemoglobin pipet, TU-1201 type visible spectrophotometer, centrifuge, normal saline, Na2CO3, 24 well culture plates, CO2 gas incubator, microplate reader etc..
Experimental technique:
1. internal organs/weight ratio pH-value determination pH: after tested material 30 days, weigh spleen, thymus, calculates dirty/body ratio.
2. delayed allergy (foot the sole of the foot thicken method): to tested material 30 days, the 25th day lumbar injection 2%SRBC, after immunity 4 days, measures left back sufficient sole of the foot portion thickness, injects 20%SRBC20ul simultaneously in measuring point, again measure after 24 hours.
The mouse spleen lymphocyte conversion test (mtt assay) of 3.ConA induction: to tested material 30 days, aseptic takes spleen, makes cell suspension, and Hank ' s liquid is washed 3 times, and adjusting cell concentration is 3 × 106Individual/ml.Cell suspension point holes being added in 24 well culture plates, every hole 1ml, a hole adds 75ul ConA liquid (100ug/ml), and another hole is comparison, puts 5%CO2, 37 DEG C of incubators to be cultivated 72h, cultivates and terminate front 4h, every hole sucks supernatant 0.7ml, adds the 0.7ml RPMI1640 culture fluid without calf serum, is simultaneously introduced MTT (5mg/ml) 50ul/ hole, continues to cultivate 4h.After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, and piping and druming uniformly, makes purple crystal be completely dissolved, and measures optical density value with 570nm wavelength.
4. half hemolysis value (HC50) mensuration: to tested material 30 days, in the 25th day lumbar injection 2%SRBC, after immunity 5 days, in collecting serum, test tube, add the serum 1ml of 300 times of dilutions, 10%SRBC0.5ml, complement 1ml, 37 DEG C of water-baths 20 minutes, ice bath terminates reaction, centrifugal, takes supernatant 1ml, add Dou Shi reagent 3ml, 540nm colorimetric after 10 minutes.
5. antibody-producting cell detection: to tested material 30 days, in the 25th day lumbar injection 2%SRBC, after immunity 5 days, dislocation is put to death, and takes spleen, makes cell suspension, 200 eye mesh screens filter, and mix with Han ' s liquid, subpackage small test tube, often pipe 0.5ml, adds 50ul10%SRBC, 20ul splenocyte suspension, mixing, reviewing, CO2 gas incubator is cultivated 1 hour, add the complement (1: 10) with the dilution of SA buffer, continue to cultivate 1 hour, count hemolysis plaque number.
6. mice carbonic clearance experiment: after tested material 30 days, the india ink (every 10 grams of body weight 0.1ml) of tail vein injection 3.5 times dilution, take blood 20ul respectively at the 2nd, 10 minutes endocanthions, join in 2.98ml0.1% sodium carbonate liquor, 600nm colorimetric.
7. Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment (half intracorporal method): after tested material 30 days, every Mus lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30min, cervical dislocation puts to death animal, faced upward position to be fixed on Mus plate, abdominal wall skin is cut off in center, it is injected into normal saline 2ml through abdominal cavity, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml, average mark drips on 2 microscope slides, put into the enamel tray being lined with wet gauze, in 37 DEG C of incubator incubation 30min, incubate complete, normal saline rinses, dry, fix with 1: 1 acetone methanol solution, 4%Giemsa dyes 10min.The macrophage number counting phagocytosis chicken red blood cell and the macrophage number swallowed.
8.NK cytoactive detection (determination of lactate dehydrogenase method): before test, YAC-1 cell (target cell) is carried out Secondary Culture by 24h, adjusting cell concentration with RPMI1640 complete culture solution is 4 × 105Individual/ml.Aseptic taking spleen, make single cell suspension, Hank ' s liquid is washed 3 times, and adjusting cell concentration is 2 × 107Individual/ml. takes target cell and each 100ul of effector lymphocyte (effect target ratio 50: 1), adding U-shaped 96 well culture plates, target cell Spontaneous release hole adds target cell and each 100ul of culture fluid, and target cell maximum release aperture adds target cell and each 100ul of 1%NP40, it is all provided with three multiple holes, in 37 DEG C, 5%CO2Cultivating 4h in incubator, in 96 well culture plates at the bottom of the Aspirate supernatant 100ul horizontalization of every hole, be simultaneously introduced LDH base fluid 100ul, react 3min, every hole adds the HCl30ul of 1mol/L, measures optical density value in microplate reader 492nm.
Result is added up: experimental data SPSS11.5for windows carries out statistical test, and matched group and experimental group use variance analysis, as heterogeneity of variance person uses data to change, the most uneven after conversion, use nonparametric statistics.
Experimental result:
1. the inventive samples impact on Mouse Weight
The impact (means standard deviation) on immune 1 group of Mouse Weight of table 1 tested material
The impact (means standard deviation) on immune 2 groups of Mouse Weights of table 2 tested material
The impact (means standard deviation) on immune 3 groups of Mouse Weights of table 3 tested material
The impact (means standard deviation) on immune 4 groups of Mouse Weights of table 4 tested material
From table 1-4, after 30 days, each treated animal vegetative activity is good, and each dosage group and the weightening finish of normal control group 1-2 mice are compared with Basal control group, the equal not statistically significant of difference (P ＞ 0.05).
2. the present invention impact on mice organs/body weight ratio
From table 5, each dosage group and normal control group 1-2 mouse spleen, thymus/body weight ratio compare with Basal control group, the equal not statistically significant of difference (P ＞ 0.05).
The impact (means standard deviation) on mice organs/body weight ratio of table 5 tested material
3. the present invention impact on mouse cell immunologic function
The 3-1. present invention impact (the foot sole of the foot thickens method) on mice delayed allergy
From table 6, before and after middle and high dosage group mouse challenge, foot sole of the foot thickness difference is higher than Basal control group, and difference statistically significant (respectively P ＜ 0.05 and P ＜ 0.01), low dose group with before and after normal control group 1-2 mouse challenge foot sole of the foot thickness difference compared with Basal control group, no significant difference (P ＞ 0.05).
The impact (means standard deviation) on mice delayed allergy (the foot sole of the foot thickens method) of table 6 tested material
(compare with Basal control group, * P ＜ 0.05；* P ＜ 0.01)
The 3-2. present invention impact on ConA inducing mouse Splenic vein hemodynamics test (mtt assay)
From table 7, middle and high dosage group mouse spleen lymphocyte converts higher than Basal control group, and difference statistically significant (respectively P ＜ 0.05 and P ＜ 0.01), in, low dose group and normal control group 1-2 contrast with Basal control group, no significant difference (P ＞ 0.05).
The impact on ConA inducing mouse Splenic vein hemodynamics test (mtt assay) of table 7 tested material
(compare with Basal control group, * P ＜ 0.05；* P ＜ 0.01)
4. the present invention impact on humoral immunization
The 4-1. present invention is to mice half hemolysis value (HC50) impact
From table 8, the mice half hemolysis value (HC of middle and high dosage group50) higher than Basal control group, and difference statistically significant (P ＜ 0.05), low dose group and normal control group 1-2 mice half hemolysis value (HC50) compared with Basal control group, no significant difference (P ＞ 0.05).
Table 8 tested material is to mice half hemolysis value (HC50) impact (means standard deviation)
(comparing with Basal control group, * P ＜ 0.05)
The impact that mouse antibodies cellulation is tested by the 4-2. present invention
From table 9, the mouse antibodies cellulation number of middle and high dosage group is higher than Basal control group, and difference statistically significant (P ＜ 0.05), low dose group and normal control group 1-2 mouse antibodies cellulation number compared with Basal control group, no significant difference (P ＞ 0.05).
The impact (means standard deviation) on mouse antibodies cellulation of table 9 tested material
(comparing with Basal control group, * P ＜ 0.05)
5. the present invention impact on monocytes/macrophages function
The impact that mice carbonic clearance is tested by the 5-1. present invention
From table 10, the mice phagocytic index of basic, normal, high dosage group is higher than Basal control group, and difference statistically significant (respectively P ＜ 0.05, P ＜ 0.01 and P ＜ 0.01), the phagocytic index of low dose group and normal control group 1-2 mice is difference thing statistical significance (P ＞ 0.05) compared with Basal control group.
The impact (means standard deviation) that mice carbonic clearance is tested by table 10 tested material
(comparing with Basal control group, * P ＜ 0.05**P ＜ 0.01)
The 5-2. present invention impact on Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell test
From table 11, three dosage groups and the phagocytic percentage of normal control group 1-2 mice and phagocytic index compared with Basal control group, the equal not statistically significant of difference (P ＞ 0.05).
The impact (means standard deviation) on Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell test of table 11 tested material
6. the present invention impact on NK cytoactive
From table 12, the NK cytoactive of middle and high dosage group is higher than Basal control group, and difference statistically significant (P ＜ 0.05), low dose group and normal control group 1-2 compared with Basal control group, the equal not statistically significant of difference (P ＞ 0.05).
The impact (means standard deviation) on NK cells in mice activity of table 12 tested material
(comparing with Basal control group, * P ＜ 0.05)
According to the experimental technique (correlation technique in " health food inspection and assessment technique specification " (version in 2003)) of test example 1, the above results demonstrates that this sample has qi and blood tonifying, strengthens the function of animal immunizing power；Show with the comparative result of Basal control group, the sample of normal control group 1-2 does not the most have the significant difference on statistical significance on all immune indexes, and the sample of the present invention has the significant difference on statistical significance on most immune indexes, after proving the crude drug combination of the present invention, create synergism, there is unforeseeable technique effect.
Claims (6)
1. a Traditional Chinese medicine health-preserving preparation with qi and blood tonifying, enhancing immunity function, it is characterised in that by the crude drug system of following part by weight
Become: Colla Corii Asini 5-25, Radix Codonopsis 15-35, Radix Angelicae Sinensis 10-30, Radix Astragali 10-30, Fructus Lycii 5-25, Cornu Cervi Pantotrichum 1-5, heme iron 1-5.
Traditional Chinese medicine health-preserving preparation the most according to claim 1, it is characterised in that be made up of the crude drug of following part by weight: Colla Corii Asini 10-20,
Radix Codonopsis 20-30, Radix Angelicae Sinensis 15-25, Radix Astragali 15-25, Fructus Lycii 10-20, Cornu Cervi Pantotrichum 2-3, heme iron 3-4.
Traditional Chinese medicine health-preserving preparation the most according to claim 1 and 2, it is characterised in that be made up of the crude drug of following part by weight: Colla Corii Asini
15, Radix Codonopsis 25, Radix Angelicae Sinensis 20, the Radix Astragali 20, Fructus Lycii 15, Cornu Cervi Pantotrichum 2, heme iron 3.
4. according to the preparation method of the Traditional Chinese medicine health-preserving preparation described in claim 1-3 any one, it is characterised in that comprise the following steps:
(1) weigh Radix Codonopsis, Radix Angelicae Sinensis, the Radix Astragali, Fructus Lycii, Cornu Cervi Pantotrichum qualified decoction pieces water boiling and extraction 2-3 time by weight proportion, add the water of 8-10 times amount every time,
2-3 hour extraction time, filter, merging filtrate, and high speed centrifugation remove impurity must refine extracting solution；Then be concentrated in vacuo, vacuum be 0.06～
0.08MPa, obtains thick extractum when measuring relative density 1.20～1.25 at 60 DEG C；Thick extractum carries out being vacuum dried and pulverize and to obtain Chinese medicinal components extract
Powder, standby；
(2) weigh Colla Corii Asini and be ground into fine powder, weigh heme iron, standby；
(3) by above-mentioned Chinese medicinal components extract powder, donkey-hide gelatin fine powder, heme iron mixing, mixture powder is obtained.
The preparation method of Traditional Chinese medicine health-preserving preparation the most according to claim 4, it is characterised in that further comprising the steps of: by step (3) gained
Mixture powder mix homogeneously with maltodextrin, acesulfame potassium, pelletize, be dried, granulate, obtain sample particle agent.
6. according to the answering in the medicine preparing qi and blood tonifying, enhancing immunity function of the Traditional Chinese medicine health-preserving preparation described in claim 1-3 any one
With.
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| CN104257813B (en) * | 2014-09-05 | 2016-09-14 | 博白县顿谷镇梁家贵种植场 | A kind of Chinese medicine composition of QI and blood regulating and preparation method thereof |
| CN104644859A (en) * | 2015-02-06 | 2015-05-27 | 西双版纳版纳药业有限责任公司 | Dai medicine preparation for immunity enhancement and body building and preparation method thereof |
| CN104857272A (en) * | 2015-05-05 | 2015-08-26 | 蔡成法 | Qi supplementing and blood nourishing testosterone raising soup and preparation method thereof |
| CN105901694A (en) * | 2016-04-13 | 2016-08-31 | 湖南楚明华医药有限公司 | Health food composition for enhancing immunity and production process thereof |
| CN106074974A (en) * | 2016-07-19 | 2016-11-09 | 成都嘉宝祥生物科技有限公司 | A kind of compositions improving immunity |
| CN109793831A (en) * | 2017-11-17 | 2019-05-24 | 孙立民 | Ginseng and pilose antler qi and blood nourishing medicinal granules |
| CN109432409A (en) * | 2018-12-19 | 2019-03-08 | 上海康孕企业管理合伙企业（有限合伙） | Application of the ferroheme in the drug, Food and hygienical food of improvement dysmenorrhea |
| CN109453264A (en) * | 2018-12-27 | 2019-03-12 | 佛山科学技术学院 | A kind of compound Chinese medicinal preparation and preparation method thereof improving immunity |
| CN110169970A (en) * | 2019-06-28 | 2019-08-27 | 上海康孕企业管理合伙企业（有限合伙） | Application of the ferroheme in the involutional drug of improvement, Food and hygienical food |
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