CN114468314A - Chicory modified dietary fiber and preparation method and application thereof - Google Patents
Chicory modified dietary fiber and preparation method and application thereof Download PDFInfo
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- CN114468314A CN114468314A CN202210142869.4A CN202210142869A CN114468314A CN 114468314 A CN114468314 A CN 114468314A CN 202210142869 A CN202210142869 A CN 202210142869A CN 114468314 A CN114468314 A CN 114468314A
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
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Abstract
The invention relates to chicory modified dietary fiber and a preparation method and application thereof. A method for preparing chicory modified dietary fiber comprises subjecting insoluble dietary fiber obtained from chicory residue to subcritical high pressure treatment. By carrying out subcritical high-pressure treatment on insoluble dietary fibers obtained from the chicory residues, the oil and water retention properties of the chicory residues can be remarkably improved, so that the chicory residues are more beneficial to the action of the chicory residues.
Description
Technical Field
The invention relates to chicory modified dietary fiber and a preparation method and application thereof.
Background
The dietary fiber is called as 'seventh nutrient', has the effects of relaxing bowel, promoting the growth of beneficial bacteria, regulating the functions of intestinal tracts, regulating blood sugar, regulating blood fat and the like, and is divided into soluble dietary fiber and insoluble dietary fiber.
Chicory is a perennial herb of the genus cichorium of the family compositae, and is distributed in northwest, north China, northeast and the like. Chicory has the effects of clearing away heat and toxic materials, inducing diuresis to reduce edema, invigorating stomach, etc., and is mainly used for treating damp-heat jaundice, nephritic edema, gastral cavity distending pain, inappetence, etc. Chicory root contains abundant polysaccharide, the chicory polysaccharide can not be digested and absorbed by human body, the blood sugar content can not be increased, obesity can not be caused, and the chicory root is suitable for the sweet eating requirements of special people such as diabetes patients and obesity patients; can also be used as proliferation factor of probiotics in human body, increase the amount of probiotics, inhibit the growth of harmful microorganism, maintain intestinal microecological balance, and improve immunity.
A large amount of chicory skin residue byproducts are generated in the production and processing process of chicory polysaccharide and are treated as feed raw materials at present, so that the phenomena of underestimation of value and resource waste are caused; the chicory residues are used for preparing the high-quality insoluble dietary fibers, so that the resource utilization rate of the chicory is improved, the industrial chain of the chicory is extended, and the added value of the chicory residues is increased.
Disclosure of Invention
The inventor unexpectedly finds that the subcritical high-pressure treatment on the insoluble dietary fiber obtained from the chicory residues can obviously improve the oil-holding property and the water-holding property of the insoluble dietary fiber, thereby being more beneficial to the action of the insoluble dietary fiber.
A method for preparing chicory modified dietary fiber comprises subjecting insoluble dietary fiber obtained from chicory residue to subcritical high pressure treatment.
According to the embodiment of the invention, the temperature of the subcritical high-pressure treatment is 110-.
According to the embodiment of the invention, the temperature of the subcritical high pressure treatment is 130-150 ℃ or 130-140 ℃.
It has been found that the subcritical high pressure treatment in the above temperature range is more advantageous for improving the oil retention property and the water retention property.
According to the embodiment of the invention, the time of the subcritical high pressure treatment is 30-120min, specifically, 30min, 60min, 90min and 120 min.
According to the embodiment of the invention, the time of the subcritical high pressure treatment is 60-120 min.
It has been found that the subcritical high-pressure treatment in the above time range is more advantageous for improving the oil retention property and the water retention property.
According to the embodiment of the invention, the temperature of the subcritical high pressure treatment is 130-150 ℃ or 130-140 ℃; the time is 60-120min, or 90-120 min.
According to the embodiment of the invention, the temperature of the subcritical high-pressure treatment is 130-140 ℃; the time is 90-120 min.
According to the embodiment of the invention, the subcritical high-pressure treatment is carried out at 110 ℃ for 30-120min under the pressure of 6.7-15.8 MPa.
According to the embodiment of the invention, the subcritical high-pressure treatment is carried out at 120 ℃ for 30-120min under the pressure of 9.1-18.2 MPa.
According to the embodiment of the invention, the subcritical high-pressure treatment is carried out at 130 ℃ for 30-120min under the pressure of 11.3-20 MPa.
According to the embodiment of the invention, the subcritical high pressure treatment is carried out at 140 ℃ for 30-120min under the pressure of 13.5-21.9 MPa.
According to the embodiment of the invention, the subcritical high-pressure treatment is carried out at 150 ℃ for 30-120min under the pressure of 14.1-24 MPa.
In some embodiments of the invention, the relationship of pressure to temperature and time under the subcritical high pressure processing conditions is shown in table 1 below.
TABLE 1
According to the embodiment of the invention, the insoluble dietary fiber obtained from the chicory residues is subjected to subcritical high-pressure treatment and then is further dried to obtain the chicory modified dietary fiber. Specifically, freeze drying or spray drying may be employed, and may be carried out according to a method conventional in the art.
Further, after the subcritical high pressure treatment, the concentration may be performed first, and then the drying may be performed. Generally, the concentrate can be concentrated to 1/2-1/5 of the original volume.
According to the embodiment of the invention, the method further comprises the step of obtaining chicory insoluble dietary fiber from chicory residues, and specifically comprises the following steps:
mixing the chicory residue with water, and carrying out wet grinding; adding xylanase and cellulase for enzymolysis; separating to obtain precipitate after enzymolysis, namely the chicory insoluble dietary fiber.
According to the embodiment of the invention, the water addition amount is 2-10 times, preferably 3-8 times, and more preferably 5-7 times of the weight of the chicory residues.
According to the embodiment of the invention, the wet grinding is carried out to 80-150 meshes, preferably 100-120 meshes, and more preferably 100 meshes.
According to the specific embodiment of the invention, the chicory residues are mixed with 5 times of water by weight and are crushed to be 120 meshes by a wet method.
It was found that crushing under the above conditions is more advantageous for the preparation of insoluble dietary fibres, thereby improving their functionality.
According to the specific embodiment of the invention, the pH of the feed liquid is adjusted to 4-6 in the enzymolysis process.
In the present invention, generally, pH adjusting agents conventional in the art, such as sodium hydroxide, hydrochloric acid, and the like, can be used.
According to the specific embodiment of the present invention, the enzymolysis time is preferably 2 to 6 hours, and more preferably 3 hours.
According to the specific embodiment of the invention, the enzymolysis temperature is 40-60 ℃.
According to a particular embodiment of the invention, the xylanase and cellulase are added in a total amount of 0.01% to 0.1%, e.g. 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1% by weight of chicory residues. Wherein, in some examples, the moisture content of the chicory residues is 80-90%.
According to a particular embodiment of the invention, the xylanase and cellulase are in a weight ratio of (1:3) - (3:1), such as, in particular, 1:1, 1:2, 1:3, 3:1, 2:1, 3:2 or 2: 3. Researches find that the xylanase and the cellulase are adopted for enzymolysis, and the ratio of the xylanase and the cellulase is controlled, so that the fiber can be better degraded, the water-soluble dietary content is improved, the particle size of insoluble dietary fiber is reduced, and the functionality of the insoluble dietary fiber is improved.
According to the specific embodiment of the invention, the sum of the addition amounts of the xylanase and the cellulase is 0.02 percent of the weight of the chicory residues; the weight ratio of the xylanase to the cellulase is (1:3) - (3:1), specifically 1:1, 1:2, 1:3, 3:1, 2:1, 3:2 or 2: 3.
According to a particular embodiment of the invention, chicory residues are processing by-products left after inulin extraction from chicory.
Generally, chicory residue contains 80-90% of water, 1.0-2.0% of protein, 8.0-12% of dietary fiber and 0.2-0.6% of ash.
The preparation method of the chicory modified dietary fiber in the specific embodiment of the invention comprises the following steps:
1) mixing chicory residue with 3-7 times of water by weight, and wet-pulverizing to 80-150 meshes;
2) adjusting pH of the feed liquid to 4-6, adding xylanase and cellulase, performing enzymolysis at 40-60 deg.C, separating (such as centrifuging), and collecting precipitate 1;
3) and performing subcritical high-pressure treatment on the precipitate 1, concentrating and drying to obtain the chicory modified dietary fiber.
According to the embodiment of the invention, the yield of the chicory modified dietary fiber prepared by the preparation method is 85-90%.
According to the preparation method, the step 2) also comprises the step of further preparing the water-soluble dietary fiber from the liquid obtained by separation after enzymolysis.
According to the embodiment of the invention, the yield of the water-soluble dietary fiber prepared by the preparation method is 68-72%.
The invention can use chicory generally planted in the field, and has the protein content of 16-25%, the soluble sugar content of 5-15%, the crude fiber content of 10-27% and the crude fat content of 2-4.5%.
The present invention also found that the above-described subcritical high pressure treatment of insoluble dietary fibres obtained from chicory residues also reduced the fibre particle size. The reduction of the fiber particle size loosens the fiber texture, can improve the capillary attraction of the fiber, and further improves the physical adsorption of water and oil substances, thereby improving the water holding capacity and the oil holding capacity of the fiber.
The invention also finds that after the subcritical high-pressure treatment is carried out on the insoluble dietary fiber obtained from the chicory residues, part of the dietary fiber is changed into water-soluble dietary fiber, so that the function of the chicory residues is better exerted.
The invention also discloses chicory modified dietary fiber prepared by the method.
Experiments show that the chicory modified dietary fiber prepared by the method has the purity of 70-80%, and has higher oil retention and water retention.
The invention also comprises the water-soluble dietary fiber prepared by the method.
Experiments show that the purity of the water-soluble dietary fiber prepared by the method is 80-95%.
The invention also comprises the application of the chicory modified dietary fiber prepared by the method in preparing food, health-care food or medicines.
The method provided by the invention obviously improves the oil retention property and water retention property of the chicory residue insoluble dietary fiber, and improves the comprehensive utilization value of the chicory.
Experiments show that the chicory modified dietary fiber prepared by the invention is more beneficial to preventing and relieving chronic diseases such as constipation, colon cancer, obesity and the like.
The method of the invention has at least one of the following advantages:
1) the waste residue generated after the chicory water-soluble dietary fiber is extracted is comprehensively utilized, and waste is changed into valuable;
2) provides a new method for modifying dietary fiber;
3) the method is simple and easy to implement, low in cost, energy-saving and environment-friendly, and suitable for large-scale industrial production.
Drawings
FIG. 1: the effect of subcritical high pressure treatment on the particle size of the chicory modified dietary fiber.
FIG. 2: the effect of subcritical high pressure treatment on the content of soluble dietary fiber in chicory modified dietary fiber.
FIG. 3: the effect of subcritical high pressure treatment on the maintaining of oiliness of chicory modified dietary fiber.
FIG. 4: the effect of subcritical high pressure treatment on the water retention of the chicory modified dietary fiber.
FIG. 5: influence of enzymolysis treatment (dosage) on the particle size of the chicory modified dietary fiber.
FIG. 6: influence of enzymolysis treatment (ratio) on the particle size of the chicory modified dietary fiber.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
If no special indication is provided, the following method for calculating the yield of the chicory soluble dietary fiber is adopted:
the yield of the chicory soluble dietary fiber is equal to the mass of the chicory soluble dietary fiber/the mass of the chicory dietary fiber is multiplied by 100 percent
If no special indication is provided, the following method for calculating the yield of the chicory modified dietary fiber is adopted:
the yield of the chicory modified dietary fiber is equal to the mass of the chicory modified dietary fiber/the mass of the chicory dietary fiber is multiplied by 100 percent
Example 1
The embodiment provides a preparation method of chicory modified dietary fiber, which comprises the following steps:
1) crushing the chicory dregs;
2) adding 5 times of water by weight into the chicory residues crushed in the step 1), mixing, and crushing by a wet crusher to obtain 120 meshes;
3) adjusting the pH of the solution obtained in the step 2) to 5.0, adding enzyme for enzymolysis at 55 ℃ for 3h, and centrifuging to collect precipitate 1; the dosage of the enzyme is 0.02% of the weight of the chicory residue; the enzyme is xylanase and cellulase, and the weight ratio of the xylanase to the cellulase is 1: 2;
4) performing subcritical high pressure treatment (treatment at 140 deg.C for 1h) on precipitate 1 in 2), concentrating the obtained sample, and freeze drying to obtain herba Cichorii modified dietary fiber. The yield was 73.45%.
And in addition, separating the liquid obtained after enzymolysis in the step 2), and drying to obtain the chicory water-soluble dietary fiber. The yield was 90.33%.
Example 2
The embodiment provides a preparation method of chicory modified dietary fiber, which comprises the following steps:
1) crushing the chicory residues;
2) adding 5 times of water by weight into the chicory residues crushed in the step 1), mixing, and crushing by a wet crusher to obtain 120 meshes;
3) adjusting the pH of the solution obtained in the step 2) to 5.0, adding enzyme for enzymolysis at 55 ℃ for 2h, and centrifuging to collect precipitate 1; the dosage of the enzyme is 0.02% of the weight of the chicory residue; the enzyme is xylanase and cellulase, and the weight ratio of the xylanase to the cellulase is 2: 3;
4) performing subcritical high pressure treatment (treatment at 140 ℃ for 1h) on the precipitate 1 in the step 2), concentrating the obtained sample, and freeze-drying to obtain the chicory modified dietary fiber. The yield was 71.20%.
And in addition, separating the liquid obtained after enzymolysis in the step 2), and drying to obtain the chicory water-soluble dietary fiber. The yield was 88.65%.
Comparative example 1
The present comparative example provides a method for the preparation of soluble dietary fiber and insoluble dietary fiber in chicory, specifically as follows:
1) cleaning herba Cichorii, removing impurities, cleaning with roller vegetable washing machine, and shredding;
2) adding 5 times of water by weight into the cut vegetable shreds obtained in the step 1), mixing, and crushing by a wet crusher to obtain 120 meshes;
3) adjusting the pH value of the supernatant 1 obtained in the step 2) to 4.0, standing at room temperature for 1h, adding activated carbon, filtering with a plate frame, and removing impurities such as pigments.
4) And (3) passing the solution obtained in the step 3) through an ion exchange column to remove salt ions and the like. Filtering with ultrafiltration membrane with molecular weight cutoff of 3000Da to remove small molecules, concentrating, and drying
5) Concentrating the solution obtained in the step 5), and performing spray drying to obtain water-soluble dietary fiber powder.
6) Concentrating the precipitate 1 in the step 2), and freeze-drying to obtain the chicory insoluble dietary fiber.
Chicory modified dietary fibres were prepared as in example 1, except that the temperature and time of the subcritical high pressure treatment was varied. And (4) measuring the particle size of the prepared chicory modified dietary fiber.
The particle size measuring method comprises the following steps: the particle size distribution of the dietary fiber particles is measured by using a particle size analyzer, distilled water is used as a dispersion medium, and wet sampling is adopted. Taking a small amount of dietary fiber suspension, and preparing into 0.1% solution in distilled water, wherein the refractive indexes of the dietary fiber and the water are respectively 1.470 and 1.330.
The results are shown in FIG. 1.
The results indicate that subcritical high pressure treatment can destroy dietary fiber structure, with fiber particle size decreasing with increasing temperature and time. The reduction of the fiber particle size loosens the fiber texture, can improve the capillary attraction of the fiber, and further improves the physical adsorption of water and oil substances, thereby improving the water holding capacity and the oil holding capacity of the fiber.
Chicory modified dietary fibres were prepared as in example 1, except that the temperature and time of the subcritical high pressure treatment was varied. And (3) determining the content of the soluble dietary fiber in the prepared chicory modified dietary fiber.
The content of the soluble dietary fiber is determined by reference to GB 5009.88-2014 determination of dietary fiber in national food safety standard.
The calculation method comprises the following steps:
the content of the soluble dietary fiber is equal to the mass of the soluble dietary fiber in the chicory modified dietary fiber/the mass of the chicory modified dietary fiber is multiplied by 100 percent
The results are shown in FIG. 2.
The results show that subcritical high pressure treatment destroys dietary fiber structure, increases water-soluble dietary fiber content in the sample (increases water solubility) with increasing temperature and time, but decreases the increase in water-soluble dietary fiber content with increasing temperature above 140 ℃.
Chicory modified dietary fibres were prepared as in example 1, except that the temperature and time of the subcritical high pressure treatment was varied. And (3) determining the oil retention property of the prepared chicory modified dietary fiber.
Method for measuring oil retention: accurately weighing 1.0g of sample (m)0) Adding 30mL rapeseed oil into a 50mL centrifuge tube, shaking uniformly, whirling and shaking at room temperature for 2h, centrifuging at 5000r/min for 15min, removing supernatant, absorbing residual oil on the wall of the centrifuge tube with oil absorption paper, and recording the mass m of a wet sample1。
Oil holding capacity (g/g) ═ m1/m0
The results are shown in FIG. 3. Wherein the oil-holding capacity of the chicory modified dietary fiber prepared in example 1 is 6.07g/g, and the oil-holding capacity of the chicory insoluble dietary fiber prepared in comparative example 1 is 4.99 g/g.
The results show that the oil retention of the treated sample increases with increasing temperature and time, but that the oil retention of the sample decreases with increasing temperature above 140 ℃, possibly due to gelatinization of sugars and a decrease in the functional properties of the sample due to an excessively high temperature. Oil retention at 140 ℃ is optimal. After treatment at 140 ℃, the oil retention of the sample increased by 21.62% compared to comparative example 1.
Experiment 4 Effect of subcritical high pressure treatment on Water binding of chicory modified dietary fiber
Chicory modified dietary fibres were prepared as in example 1, except that the temperature and time of the subcritical high pressure treatment was varied. And (3) measuring the water binding capacity of the prepared chicory modified dietary fiber.
The water binding capacity measuring method comprises the following steps: accurately weighing 1.0g of sample (m)0) Adding into 50mL centrifuge tube, adding 30mL distilled water, shaking, vortex shaking at room temperature for 18h, and centrifuging at 5000r/min15min, remove the supernatant, and suck the residual water on the centrifuge tube wall with absorbent paper, record the mass m of the wet sample1。
Water holding capacity (g/g) ═ m1/m0
The results are shown in FIG. 4. Wherein the water holding capacity of the chicory modified dietary fiber prepared in example 1 is 12.51g/g, and the water holding capacity of the chicory insoluble dietary fiber prepared in comparative example 1 is 9.35 g/g.
The results show that the water holding capacity of the treated sample increases with increasing temperature and time, but that the oil holding capacity of the sample decreases with increasing temperature above 140 ℃, possibly due to gelatinization of sugars and a decrease in the functional properties of the sample caused by an excessively high temperature. The water retention is optimal at 140 ℃. After treatment at 140 ℃, the oil retention of the sample increased by 33.77% compared to comparative example 1.
The chicory dregs are respectively subjected to common grinding and wet grinding (the method is the same as that in example 1), and then are subjected to enzyme treatment (5 times of water by weight is added, enzymolysis is carried out for 3 hours under the natural pH condition, 55 ℃), and the refractivity of the supernatant of the solution after the enzymolysis is measured. Wherein the dosage of the enzyme is 0.1 percent and 0.01 percent of the weight of the chicory residue respectively, the enzyme is xylanase and cellulase, and the weight ratio of the xylanase to the cellulase is 1: 1.
The results are shown in Table 2.
The results show that wet grinding and enzymolysis are combined, so that the enzymolysis efficiency can be higher, the sugar content in the solution can be increased, and the wet grinding mode and enzymolysis can change the fiber structure and carry out enzymolysis on the fibers.
TABLE 2 refractive index of the supernatant of the solution after enzymatic hydrolysis
The chicory residues are respectively ground by a wet method (the method is the same as the example 1), and then are treated by enzyme (5 times of water by weight is added, the chicory residues are subjected to enzymolysis for 3 hours under the natural pH condition, the temperature is 55 ℃), and the particle size of the chicory modified dietary fibers after the enzymolysis is measured.
Wherein the dosage of the enzyme is 0.05 percent, 0.1 percent, 0.15 percent, 0.20 percent, 0.25 percent and 0.30 percent of the weight of the chicory residue respectively. The enzyme is xylanase and cellulase, and the weight ratio of the xylanase to the cellulase is 1: 1.
The results are shown in FIG. 5.
The results show that: the particle size of the chicory modified dietary fiber can be reduced along with the increase of the enzyme addition amount, but when the enzyme addition amount is more than or equal to 0.1%, the particle size of the chicory modified dietary fiber is less than 200nm, but the influence on the particle size is not obvious along with the increase of the enzyme addition amount.
The chicory residues are respectively ground by a wet method (the method is the same as the example 1), and then are treated by enzyme (5 times of water by weight is added, the chicory residues are subjected to enzymolysis for 3 hours under the natural pH condition, the temperature is 55 ℃), and the particle size of the chicory modified dietary fibers after the enzymolysis is measured. Wherein the dosage of the enzyme is 0.02% of the weight of the chicory residues, and the enzyme is xylanase and cellulase in a weight ratio of 1:1, 1:2, 2:1, 2:3 and 3: 2.
The results are shown in FIG. 6.
The results show that: different ratios of enzymes affect the particle size of chicory modified dietary fibre, as can be seen from figure 6, the effect on particle size is greatest when the xylanase and cellulase ratio is 3: 2.
Claims (10)
1. A method for preparing chicory modified dietary fiber is characterized by comprising the step of carrying out subcritical high-pressure treatment on insoluble dietary fiber obtained from chicory residues.
2. The method for preparing chicory modified dietary fiber as defined in claim 1, wherein the temperature of the subcritical high pressure treatment is 110-150 ℃, such as 110 ℃, 120 ℃, 130 ℃, 140 ℃, 150 ℃; and/or the presence of a gas in the atmosphere,
the temperature of the subcritical high-pressure treatment is 130-150 ℃ or 130-140 ℃; and/or the presence of a gas in the gas,
the subcritical high-pressure treatment time is 30-120min, or 60-120 min.
3. The method for preparing chicory modified dietary fiber as claimed in claim 1, wherein the temperature of the subcritical high pressure treatment is 130-150 ℃ or 130-140 ℃; the time is 60-120min, or 90-120 min.
4. A process for the preparation of chicory modified dietary fibres as claimed in claim 1, wherein the sub-critical high pressure treatment is selected from any one of the group consisting of:
i) the temperature of the subcritical high-pressure treatment is 130-140 ℃; the time is 90-120 min;
ii) the subcritical high-pressure treatment is carried out at 110 ℃ for 30-120min under the pressure of 6.7-15.8 MPa;
iii) the subcritical high-pressure treatment is carried out at 120 ℃ for 30-120min under the pressure of 9.1-18.2 MPa;
iv) the subcritical high-pressure treatment is carried out at 130 ℃ for 30-120min under the pressure of 11.3-20 MPa;
v) the subcritical high-pressure treatment is carried out at 140 ℃ for 30-120min under the pressure of 13.5-21.9 MPa;
vi) the subcritical high pressure treatment is carried out at 150 ℃ for 30-120min under the pressure of 14.1-24 MPa.
5. A process for the preparation of chicory modified dietary fibres as claimed in any one of claims 1 to 4, wherein the process further comprises the step of obtaining chicory insoluble dietary fibres from chicory residue, in particular comprising:
mixing the chicory residue with water, and carrying out wet grinding; adding xylanase and cellulase for enzymolysis; separating to obtain precipitate after enzymolysis, namely the chicory insoluble dietary fiber.
6. A process for the preparation of chicory modified dietary fibre according to claim 5, characterised in that the amount of water added is 2-10 times, preferably 3-8 times, more preferably 5-7 times the weight of chicory residues; and/or the presence of a gas in the gas,
wet pulverizing to 80-150 mesh, preferably 100-120 mesh, more preferably 100 mesh.
7. A process for the preparation of chicory modified dietary fibres as claimed in claim 5 or 6, wherein the sum of the amounts of xylanase and cellulase added is 0.01% to 0.1% by weight of chicory residue, such as 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%;
alternatively, the xylanase and cellulase are present in a weight ratio of (1:3) - (3:1), specifically, e.g., 1:1, 1:2, 1:3, 3:1, 2:1, 3:2, or 2: 3;
optionally, the sum of the addition amounts of the xylanase and the cellulase is 0.02 percent of the weight of the chicory dregs; the weight ratio of the xylanase to the cellulase is (1:3) - (3:1), specifically 1:1, 1:2, 1:3, 3:1, 2:1, 3:2 or 2: 3.
8. A process for the preparation of chicory modified dietary fibres as claimed in any one of claims 1 to 7, which comprises:
1) mixing chicory residue with 3-7 times of water by weight, and wet-pulverizing to 80-150 meshes;
2) adjusting pH of the feed liquid to 4-6, adding xylanase and cellulase, performing enzymolysis at 40-60 deg.C, separating, and collecting precipitate 1;
3) and performing subcritical high-pressure treatment on the precipitate 1, concentrating and drying to obtain the chicory modified dietary fiber.
9. A chicory modified dietary fibre produced by the process of any one of claims 1 to 8; optionally, the chicory modified dietary fibre has a purity of 70-80%.
10. Use of chicory modified dietary fibre according to claim 9 for the preparation of a food, nutraceutical or pharmaceutical product.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116172209A (en) * | 2023-02-23 | 2023-05-30 | 华南理工大学 | Almond meal insoluble dietary fiber, preparation method and application thereof in reducing blood sugar |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142979A (en) * | 2006-09-14 | 2008-03-19 | 杭州浙大力夫生物科技有限公司 | High-quality insoluble plant edible fiber micropowder and preparation method and application thereof |
| CN101933613A (en) * | 2010-08-18 | 2011-01-05 | 国家林业局竹子研究开发中心 | Enzymolysis method for extracting insoluble dietary fiber from bamboo sprout |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN110916199A (en) * | 2018-09-19 | 2020-03-27 | 浙江省农业科学院 | Modification method of dietary fiber and obtained product |
| CN112314954A (en) * | 2020-11-17 | 2021-02-05 | 唐山市食品药品综合检验检测中心(唐山市畜牧水产品质量监测中心) | Preparation method of corn dietary fiber |
| CN113331430A (en) * | 2021-05-17 | 2021-09-03 | 仲恺农业工程学院 | Method for preparing soluble dietary fiber from citrus fruit peel residues |
-
2022
- 2022-02-16 CN CN202210142869.4A patent/CN114468314A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142979A (en) * | 2006-09-14 | 2008-03-19 | 杭州浙大力夫生物科技有限公司 | High-quality insoluble plant edible fiber micropowder and preparation method and application thereof |
| CN101933613A (en) * | 2010-08-18 | 2011-01-05 | 国家林业局竹子研究开发中心 | Enzymolysis method for extracting insoluble dietary fiber from bamboo sprout |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN110916199A (en) * | 2018-09-19 | 2020-03-27 | 浙江省农业科学院 | Modification method of dietary fiber and obtained product |
| CN112314954A (en) * | 2020-11-17 | 2021-02-05 | 唐山市食品药品综合检验检测中心(唐山市畜牧水产品质量监测中心) | Preparation method of corn dietary fiber |
| CN113331430A (en) * | 2021-05-17 | 2021-09-03 | 仲恺农业工程学院 | Method for preparing soluble dietary fiber from citrus fruit peel residues |
Non-Patent Citations (2)
| Title |
|---|
| 方政: "超微粉碎—酶解锦橙皮渣制取天然饮料混浊剂的研究", 中国优秀博硕士学位论文全文数据库 (硕士)工程科技Ⅰ辑, no. 05, 15 September 2005 (2005-09-15), pages 024 - 158 * |
| 王俊丽: "膳食纤维改性研究进展", 食品研究与开发, vol. 33, no. 5, 31 May 2012 (2012-05-31), pages 225 - 228 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116172209A (en) * | 2023-02-23 | 2023-05-30 | 华南理工大学 | Almond meal insoluble dietary fiber, preparation method and application thereof in reducing blood sugar |
| CN116172209B (en) * | 2023-02-23 | 2024-05-03 | 华南理工大学 | Almond meal insoluble dietary fiber, preparation method and application thereof in reducing blood sugar or assisting in reducing blood sugar |
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