CN101899120A - Method for refining jujube polysaccharide - Google Patents
Method for refining jujube polysaccharide Download PDFInfo
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- CN101899120A CN101899120A CN2010101022245A CN201010102224A CN101899120A CN 101899120 A CN101899120 A CN 101899120A CN 2010101022245 A CN2010101022245 A CN 2010101022245A CN 201010102224 A CN201010102224 A CN 201010102224A CN 101899120 A CN101899120 A CN 101899120A
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- jujube
- polysaccharide
- refining
- decolouring
- alcohol precipitation
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 76
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 76
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 76
- 238000007670 refining Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 19
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 241001247821 Ziziphus Species 0.000 claims abstract description 53
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 235000020418 red date juice Nutrition 0.000 claims abstract description 16
- 238000005516 engineering process Methods 0.000 claims abstract description 15
- 238000001556 precipitation Methods 0.000 claims abstract description 15
- 238000005238 degreasing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000010298 pulverizing process Methods 0.000 claims abstract description 4
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- 238000004061 bleaching Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 230000003544 deproteinization Effects 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229920001491 Lentinan Polymers 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000008504 concentrate Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229940115286 lentinan Drugs 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000000452 restraining effect Effects 0.000 description 2
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 description 1
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241001061264 Astragalus Species 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 229920001543 Laminarin Polymers 0.000 description 1
- 239000005717 Laminarin Substances 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229940006091 aloe polysaccharide Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 229920001206 natural gum Polymers 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 210000004233 talus Anatomy 0.000 description 1
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Abstract
The invention relates to a method for refining a jujube polysaccharide. The jujube polysaccharide is produced from coarse polysaccharide obtained in the production process of concentrating jujube juice, and the coarse polysaccharide is subjected to water dissolution, degreasing, deproteinization, decoloration, alcohol precipitation, freeze drying, pulverizing and packaging and code spraying to obtain the refined jujube polysaccharide, wherein the production method of concentrating the jujube juice comprises the following steps of: performing cleaning, low-temperature rehydration, cold pulping, enzymolysis and separation on jujube raw materials to obtain jujube pits, jujube dregs and the jujube juice respectively; and performing decoloration and ultrafiltration on the jujube juice to obtain permeating liquid and trapping liquid, and performing the alcohol precipitation and separation on the trapping liquid to obtain a precipitate, namely the coarse polysaccharide, wherein golden-silk jujubes are taken as the jujube raw materials. In the method for refining the jujube polysaccharide, the jujubes are subjected to biological extraction effectively, and the conventional technology of extracting the polysaccharide is combined with the advanced bioengineering technology, so a process is advanced, energy consumption is reduced, the polysaccharide content of products is over 95 percent, and the method provides an effective way for the refining processing of the jujubes.
Description
Technical field
The present invention relates to the deep processing of farm products technology, particularly a kind of method for refining jujube polysaccharide.
Background technology
Polysaccharide and protein, gene are three big fields of life science, the research that the back is two has obtained huge achievement in 20th century, and become field, 21 century life science forward position as the polysaccharide that can make life shine extraordinary splendor, its development and use have been caused the extensive concern of countries in the world.Polyose claims saccharan (Polysaccharides) again, molecular weight is at tens of thousands or millions of, vegetable polysaccharides comprises starch, Mierocrystalline cellulose, dextran (as lentinan), Polylevulosan, natural gum, phlegmatic temperament, viscose glue fat (as agar) etc., and wherein some immuno-stimulating polysaccharide is the cancer cells inhibitor.
Vegetable polysaccharides has restraining effect to diabetes, stomach ulcer, pains and other diseases, also has the effect that reduces serum cholesterol, promotes growth of animal.The specificity and the non-specific immunity of vegetable polysaccharides energy enhancing body directly or indirectly activate bone-marrow-derived lymphocyte, T lymphocyte and scavenger cell, and difficult diseases such as tumour, diabetes, hyperlipidemia, hypercholesterolemia are had treatment and restraining effect.
Present domestic vegetable polysaccharides is various in style, as lentinan, astragalus polysaccharides, laminarin, squash polyoses, Aloe polysaccharide or the like, along with the bioactive research of polysaccharide being goed deep into the biological activity mechanism of polysaccharide, efficacy factor can be clearer and more definite, and its Application Areas also will be widened more.
In recent years, along with improving constantly of standard of living, people's health care consciousness was strengthening gradually.Polysaccharide quietly rises as the main component of protective foods.China's polysaccharide aboundresources, applicating history is long, has huge DEVELOPMENT PROSPECT.By the structure of polysaccharide being imitated and the research of dose-effect relationship, people will utilize the polysaccharide resource of China's abundant, what produce high added value is the protective foods of major ingredient with polysaccharide (or compound polyose), to improve people's body constitution, to improve health, improve the quality of living.
Jujube is a main farm produce of China, and distributed areas are wide, and output is big, and the jujube polysaccharide is a kind of natural plant polyose.
Summary of the invention
For making full use of the jujube resource, the invention provides a kind of method for refining jujube polysaccharide.
Method for refining jujube polysaccharide of the present invention is utilize to concentrate the Crude polysaccharides that jujube juice production process obtains to produce, and wherein: described Crude polysaccharides obtains refining jujube polysaccharide after through water-soluble, degreasing, deproteinated, decolouring, alcohol precipitation, lyophilize, pulverizing, and concrete steps are as follows:
(1) deionized water dissolving, degreasing
With the Crude polysaccharides deionized water dissolving, adopt ethanol, ether hybrid system to remove lipoid material wherein;
(2) deproteinated
The employing trichoroacetic acid(TCA) is removed protein wherein;
(3) decolouring
Polysaccharide soln color behind degreasing, the deproteinated is light yellow, adopts the activated carbon decolorizing technology, makes polysaccharide soln reach water white transparency, and activated carbon dosage is 0.7%, 60 ℃ of bleaching temperatures, bleaching time 1 hour;
(4) alcohol precipitation
After the decolouring activated carbon filtration is fallen, adopt 95% ethanol that the Golden jujube polysaccharide precipitation is come out;
(5) lyophilize
Adopt Vacuum Freezing ﹠ Drying Technology that the moisture content of Golden jujube polysaccharide is reduced to below 5%, Vacuum Freezing ﹠ Drying Technology can at utmost keep the activity of polysaccharide;
(6) pulverize
Polysaccharide after the freeze-drying is pulverized;
(7) packing coding.
Described concentrated jujube juice production method comprises: the jujube raw material obtains jujube nuclear, jujube slag and jujube juice respectively through cleaning, rehydration, making beating, enzymolysis, separation, jujube juice obtains seeing through liquid and trapped fluid through decolouring, ultrafiltration, and it is Crude polysaccharides that trapped fluid obtains throw out through alcohol precipitation, separation.
Described jujube raw material is selected Golden jujube for use.
Method for refining jujube polysaccharide of the present invention by jujube being carried out the biological extraction of effective effect, combines the polysaccharide technology of tradition extraction and advanced biotechnology, technology advanced person, energy consumption reduces, and product contains polysaccharide and reaches more than 95%, for the intensive processing of jujube provides valid approach.
Description of drawings
Fig. 1 is the schema of method for refining jujube polysaccharide of the present invention.
Embodiment
Below in conjunction with embodiment method for refining jujube polysaccharide of the present invention is done more detailed explanation.
Fig. 1 is the schema of method for refining jujube polysaccharide of the present invention.
Method for refining jujube polysaccharide of the present invention is to utilize the trapped fluid that concentrates the production of jujube juice production method to produce.The jujube raw material that concentrates in the jujube juice production method obtains jujube nuclear, jujube slag and jujube juice respectively through cleaning, low temperature rehydration, cold making beating, enzymolysis, separation, jujube juice obtains seeing through liquid and trapped fluid through decolouring, ultrafiltration, and it is Crude polysaccharides that trapped fluid obtains throw out through alcohol precipitation, separation.
Processing for Crude polysaccharides will be through obtaining refining jujube polysaccharide after water-soluble, degreasing, deproteinated, decolouring, alcohol precipitation, lyophilize, the pulverizing, concrete steps are as follows:
(1) deionized water dissolving, degreasing
With the Crude polysaccharides deionized water dissolving, adopt ethanol, ether hybrid system to remove lipoid material wherein;
(2) deproteinated
The employing trichoroacetic acid(TCA) is removed protein wherein;
(3) decolouring
Polysaccharide soln color behind degreasing, the deproteinated is light yellow, adopts the activated carbon decolorizing technology, makes polysaccharide soln reach water white transparency, and activated carbon dosage is 0.7%, 60 ℃ of bleaching temperatures, bleaching time 1 hour;
(4) alcohol precipitation
After the decolouring activated carbon filtration is fallen, adopt 95% ethanol that the Golden jujube polysaccharide precipitation is come out;
(5) lyophilize
Adopt Vacuum Freezing ﹠ Drying Technology that the moisture content of Golden jujube polysaccharide is reduced to below 5%, Vacuum Freezing ﹠ Drying Technology can at utmost keep the activity of polysaccharide;
(6) pulverize
Polysaccharide after the freeze-drying is pulverized;
(7) packing coding.
Golden jujube polysaccharide quality standard
Claims (5)
1. method for refining jujube polysaccharide, be that Crude polysaccharides is produced in the concentrated jujube juice production method of utilization, it is characterized in that: described Crude polysaccharides obtains refining jujube polysaccharide after through water-soluble, degreasing, deproteinated, decolouring, alcohol precipitation, lyophilize, pulverizing, and concrete steps are as follows:
(1) deionized water dissolving, degreasing
With the Crude polysaccharides deionized water dissolving, adopt ethanol, ether hybrid system to remove lipoid material wherein;
(2) deproteinated
The employing trichoroacetic acid(TCA) is removed protein wherein;
(3) decolouring
Polysaccharide soln color behind degreasing, the deproteinated is light yellow, adopts the activated carbon decolorizing technology, makes polysaccharide soln reach water white transparency;
(4) alcohol precipitation
After the decolouring activated carbon filtration is fallen, adopt ethanol that the Golden jujube polysaccharide precipitation is come out;
(5) lyophilize
Adopt Vacuum Freezing ﹠ Drying Technology that the moisture content of Golden jujube polysaccharide is reduced to below 5%, Vacuum Freezing ﹠ Drying Technology can at utmost keep the activity of polysaccharide;
(6) pulverize
Polysaccharide after the freeze-drying is pulverized;
(7) packing coding.
2. method for refining jujube polysaccharide according to claim 1 is characterized in that: activated carbon dosage is 0.7% in the described decolouring step, 60 ℃ of bleaching temperatures, bleaching time 1 hour.
3. method for refining jujube polysaccharide according to claim 2 is characterized in that: in the described alcohol precipitation step, ethanol adopts 95% ethanol.
4. method for refining jujube polysaccharide according to claim 3, it is characterized in that: described concentrated jujube juice production method comprises: the jujube raw material obtains jujube nuclear, jujube slag and jujube juice respectively through cleaning, low temperature rehydration, cold making beating, enzymolysis, separation, jujube juice obtains seeing through liquid and trapped fluid through decolouring, ultrafiltration, and it is Crude polysaccharides that trapped fluid obtains throw out through alcohol precipitation, separation.
5. method for refining jujube polysaccharide according to claim 4 is characterized in that: described jujube raw material is selected Golden jujube for use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101022245A CN101899120B (en) | 2010-01-28 | 2010-01-28 | Method for refining jujube polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101022245A CN101899120B (en) | 2010-01-28 | 2010-01-28 | Method for refining jujube polysaccharide |
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| Publication Number | Publication Date |
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| CN101899120A true CN101899120A (en) | 2010-12-01 |
| CN101899120B CN101899120B (en) | 2012-09-26 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987482A (en) * | 2012-11-29 | 2013-03-27 | 新疆天海绿洲农业科技股份有限公司 | Concentrated red date juice and preparation method thereof |
| CN103421125A (en) * | 2012-05-15 | 2013-12-04 | 周鑫 | Comprehensive utilization method of jujube residue |
| CN103755829A (en) * | 2014-02-18 | 2014-04-30 | 中国林业科学研究院林产化学工业研究所 | Preparation method of Huping jujube polysaccharide |
| CN105884924A (en) * | 2015-01-25 | 2016-08-24 | 中国林业科学研究院林产化学工业研究所 | Technological method for ultrasonic spray drying of red date polysaccharide solution |
| CN107082822A (en) * | 2017-05-28 | 2017-08-22 | 中国热带农业科学院南亚热带作物研究所 | A kind of mango processing byproduct polysaccharide takes off albumen and discoloration method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358219B (en) * | 2008-07-14 | 2011-06-08 | 天津农学院 | Simultaneous sequential chemical extraction of jujube flavones and jujube polysaccharide by fermentation method |
-
2010
- 2010-01-28 CN CN2010101022245A patent/CN101899120B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421125A (en) * | 2012-05-15 | 2013-12-04 | 周鑫 | Comprehensive utilization method of jujube residue |
| CN102987482A (en) * | 2012-11-29 | 2013-03-27 | 新疆天海绿洲农业科技股份有限公司 | Concentrated red date juice and preparation method thereof |
| CN103755829A (en) * | 2014-02-18 | 2014-04-30 | 中国林业科学研究院林产化学工业研究所 | Preparation method of Huping jujube polysaccharide |
| CN105884924A (en) * | 2015-01-25 | 2016-08-24 | 中国林业科学研究院林产化学工业研究所 | Technological method for ultrasonic spray drying of red date polysaccharide solution |
| CN107082822A (en) * | 2017-05-28 | 2017-08-22 | 中国热带农业科学院南亚热带作物研究所 | A kind of mango processing byproduct polysaccharide takes off albumen and discoloration method |
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| Publication number | Publication date |
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| CN101899120B (en) | 2012-09-26 |
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