CN105815788B - A kind of health substitution sugar and the method with liquid state fermentation technology preparation health substitution sugar - Google Patents
A kind of health substitution sugar and the method with liquid state fermentation technology preparation health substitution sugar Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of health substitution sugar and the method with liquid state fermentation technology preparation health substitution sugar.The present invention relates to food technology fields, more particularly to a kind of health substitution sugar and the method for preparing health substitution sugar using liquid state fermentation technology, including following components several by mass percentage: L-arabinose 40 ~ 90%；Fructose 10 ~ 60%；Xylose 0 ~ 10%；Glucose 0 ~ 10%.The preparation method comprises the following steps: the preparation of maize peel enzymolysis liquid and jerusalem artichoke juice；Spawn incubation；Fermented and cultured；Centrifugal filtration is decolourized, and is concentrated under reduced pressure, and substitution syrup is made.Its syrup is centrifuged, and spray drying may also be fabricated which substitution Icing Sugar.Health substitution sugar obtained is suitable for prohibiting sucrose crowd and vast sweet tooth and is afraid of that unhealthful crowd is edible.Required raw material sources are cheap extensively, and physico chemical factor is easily controllable, with short production cycle, and stable product quality yield is high, are convenient for industrialized production, and do not controlled by season.
Description
Technical field
The present invention relates to food technology fields, prepare more particularly to a kind of health substitution sugar and using liquid state fermentation technology
The method of health substitution sugar.
Background technique
Sugar is one of important substance for the survival of mankind, is one of big main nutrients of human body three, is human body thermal energy
Main source." sugar " generally eaten typically refers to sucrose.About 4 calories of heat contained by every gram of sugar, if intake is excessive
And can not consume in time, extra heat will be converted to fat.In addition, food, drink containing addition sugar absorb excessive meeting
Blood glucose rapid increase is enabled, insulin in blood is caused to increase；Insulin can enable body efficiently depot fat, cause fertilizer
It is fat, it will increase the risk for suffering from chronic disease such as cardiovascular disease.So some experts think: sugar is than cigarette and alcoholic drink pair
The harm of human body is also big.World Health Organization's investigation discovery, it is hard that sugar intake excessively will lead to heart disease, hypertension, blood vessel
Change disease and cerebral hemorrhage, diabetes etc..The evil of thermophilic sugar, more than smokes.Therefore, the World Health Organization proposes " to guard against in the whole world in nineteen ninety-five
The new slogan of sugar ".With the improvement of living standards, people are to high heating values such as addition sucrose, glucose and have certain side effect
Sugar is not welcome increasingly.And sucrose uses long-standing, peculiar sense of taste of the people especially children to sucrose sweet taste
Dependence is difficult to shake.How not only to have kept the habitual sucrose taste of people, but also benefited from functional sugar low sugar, nutrition etc.
New concept of health, and a problem of puzzled food service industry.
L-arabinose (L-arabinose) is also known as arabinose, is able to suppress the absorption of sucrose, 3.5 are added in sucrose
The ratio of %, is able to suppress the absorption of 60 ~ 70 % sucrose of human body, and long-time service can control blood glucose rise therefore can inhibit fertilizer
It is fat, prevent disease relevant to hyperglycemia；Prevent constipation promotes bifidobacterium growth；There is the function for changing skeletal muscle fibre ingredient
Effect influences the growth of abdominal adipose tissue to glycoxidative by dominating glycolysis, and the change of this Muscle fiber density may have
Improve the effect of diabetes B.
Fructose is a kind of refine from various fruit and cereal, new carbohydrate all natural, sweet taste is strong, because not easily leading to high blood
Sugar is also not susceptible to fat accumulation and gets fat, and will not more generate saprodontia, and is recognized by more people.The sugariness of fructose is
1.8 times of sucrose are the highest sugar of sugariness in all natural sugars, so the intake of fructose is only under same sweetness standard
For the half of sucrose.And compared with synthesis is for sugar, fructose trophism with it is then more reliable in safety.It can be said that fructose
It is current most safe, most healthy one of sugar known in the world.
Xylose is a component of xylan, is widely present in plant.Xylose is not digested absorption, without energy value,
Can meet love to the maximum extent and eat the needs of sweets worries the person of getting fat again；Edible xylose can improve the microbial environment of human body, mention
The immunocompetence of high body；Xylose is not utilized by Institute of Micro-biology in oral cavity, is had the part physiological function of dietary fiber, can be reduced
Serum cholesterol, prevention intestinal cancer etc.；The compatibility of xylose and food is fine, and a small amount of xylose is added in food, just can embody very
Good health-care effect；Xylose is taken in simultaneously with calcium, human body can be improved to the absorptivity and retention rate of calcium, moreover it is possible to prevent constipation.
With the improvement of living standards, the health that people are increasingly careful in one's diet, thus develop it is a kind of it is novel, be able to satisfy
The sweetener that people's mouthfeel is not easy to cause a variety of diseases again is extremely urgent.Currently, most of use functional sugar alcohol such as xylitol, wheat
The parts such as bud sugar alcohol substituting saccharose, but since people are in functional sugar alcohols such as edible xylitol, maltitols, it may appear that diarrhea,
The adverse consequences of abdominal distension, therefore its amount and edible crowd are partially limited.The prior art passes through melting or vacuum mostly
Several sugar are mixed with functional sugar by the methods of concentration, but such method cost of material is high, is not easy expanding production；Utilize plant
Raw material prepares arabinose, fructose etc. and usually requires the chemical methodes such as sour water solution in process of production, not only complex process, and
There is the potential risk for introducing chemical component.
Summary of the invention
Health substitution sugar is prepared the object of the present invention is to provide a kind of health substitution sugar and using liquid state fermentation technology
Method.This method is that health substitution sugar, compared with common sucrose, mouthfeel alcohol are prepared using the method for strain liquid submerged fermentation
Thickness, sugariness is moderate, will not generate the common diseases such as some diabetes, cardiovascular disease because of excessive edible, be suitable for prohibiting
Sucrose crowd and vast sweet tooth and be afraid of that unhealthful crowd is edible, thoroughly compensate for edible sucrose to people's bring
Harm.Raw materials used in preparation is secondary use raw material, simple and easy to get, environmentally protective；Instrument occupied area is small, fermentation efficiency
Height, it is easy to operate, labor intensity is substantially reduced, and do not introduce dangerous substance in operating process, reduces energy consumption, it is at low cost
It is honest and clean, it is easy to mechanization, automated production.
For this purpose, technical scheme is as follows:
A kind of health substitution sugar, including following components several by mass percentage:
40 ~ 90 % of L-arabinose
10 ~ 60 % of fructose
0 ~ 10 % of xylose
0 ~ 10 % of glucose.
A kind of above-mentioned health substitution sugar, the component counted by mass percentage are preferred are as follows:
50 ~ 70 % of L-arabinose
20 ~ 40 % of fructose
0 ~ 5 % of xylose
0 ~ 5 % of glucose.
A kind of above-mentioned health substitution sugar, the component counted by mass percentage are further preferred are as follows:
60 % of L-arabinose
35 % of fructose
3 % of xylose
2 % of glucose.
A method of above-mentioned health substitution sugar is prepared using liquid state fermentation technology, comprising the following steps:
1) prepared by maize peel enzymolysis liquid: maize peel warp smashes it through 60 meshes, is added according to the weight ratio of 1:3 ~ 1:12
Purified water reconciles pH value to 5.0 ~ 6.0, and agitation revolution is 50 ~ 120 rpm/min, is added according to 0.3 ~ 6.0 % of maize peel dry
Enter hemicellulose degradation complex enzyme, controlled enzymatic hydrolysis temperature is 45 ~ 80 DEG C, 8 ~ 40 h of enzymolysis time, then through plate and frame filter press mistake
It filters, removal waste residue, sugared weight percent concentration is 10 ~ 30 % in obtained enzymolysis liquid；
Jerusalem artichoke juice preparation: fresh jerusalem artichoke cleaning weighing, 100 DEG C of 5 min enzyme deactivations of heating add 80 DEG C of appropriate amount of water to blend, double-deck
100 mesh filter cloth filters pressings, filter residue add appropriate hot water to repeat filters pressing, and mixed filtrate constant volume to every 100 g jerusalem artichoke goes out 100 mL juice, and 110
DEG C sterilizing 30 min；
2) Spawn incubation: barms and aspergillus niger strain are inoculated in the fermentor equipped with bacterium culture medium, 1:
0.6 air quantity, 200 rpm/min of revolving speed, cultivate 24 h, obtain bacteria culture fluid by 29 DEG C of cultivation temperature；
3) it is dense that the maize peel enzymolysis liquid of step 1) preparation and jerusalem artichoke juice fermented and cultured: are concentrated into weight percent respectively
Degree is 20 ~ 40 %, as substrate, is added in the fermentor equipped with fermentation medium, and by 5 % inoculum concentrations access step 2) system
Standby bacteria culture fluid, 60 % of fermentation tank capacity, 400 r/min of mixing speed, ventilatory capacity are 1.2 ~ 2 L/min, 29 DEG C of items
24 h are cultivated under part, obtain fermentation culture；
4) fermentation culture for obtaining step 3) adds water and stirs, and 100 DEG C of 30 ~ 120 min of heating are cooled to 45 DEG C
Centrifugal filtration obtains level-one filtrate；The water centrifugal filtration again of centrifugation gained precipitating plus 2 times of amounts, obtains second level filtrate and precipitating；
Gained two-stage precipitation adds the water centrifugal filtration of 2 times of amounts again, obtains three-level filtrate；Merge centrifugation gained filtrate three times, 3 % are added
Active carbon, 80 DEG C of 30 min of decoloration, is filtered while hot, by decoloration filtrate decompression concentration, health is made and substitutes sugared syrup.
Health made from step 4) is substituted sugared syrup to separate through centrifuge, obtained solid phase is spray-dried, and is made
Health substitution Icing Sugar.
The group of the step 2) bacterium culture medium is divided into (mass percent): 1 % of yeast extract, 2 % of glucose, peptone 2
%, KH2PO40.5 %, pH are naturally, 20 min that sterilize under the conditions of 121 DEG C.
The group of the step 3) fermentation medium is divided into (mass percent): 0.2 % of yeast extract, 0.3 % of peptone,
KH2PO40.1 %, (NH4)2 SO40.5 %, MgSO4·7H2O 0.05 %, sterilize under the conditions of 121 DEG C 20 min.
The invention has the advantages that
1) activity of invertase in small intestine can selectively be inhibited using L-arabinose, to reach inhibition to sucrose
Absorption, and then prevent relevant to hyperglycemia disease, be suitable for prohibiting sucrose crowd and vast sweet tooth and be afraid of to influence to be good for
The crowd of health is edible.Edible sucrose is thoroughly compensated for endanger people's bring；
2) fructose in health substitution sugar does not easily lead to hyperglycemia, is also not susceptible to fat accumulation and gets fat, will not more produce
Raw saprodontia, a small amount of addition are able to satisfy people to taste demand；
3) required raw material sources are cheap extensively in preparation, and the nutrition distribution pattern of fluid nutrient medium is uniform, is conducive to
Mushroom trophosome comes into full contact with and absorbs；
4) during the cultivation process, physico chemical factor is easily controllable, and production technology specification, with short production cycle, product quality is steady
Fixed, yield is high, low to Preparation equipment and site requirements, is convenient for industrialized production, and do not controlled by season.
Specific embodiment
The specific embodiment of form by the following examples makees further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following embodiment.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Barms (torulopsis, number: ACCC20343), aspergillus niger strain (number: CICC41529) are purchased from micro-
Biological inoculum collection；
Bacterium culture medium component (mass percent): 1 % of yeast extract, 2 % of glucose, peptone 2 %, KH2PO40.5 %,
PH is naturally, 20 min that sterilize under the conditions of 121 DEG C；
The component (mass percent) of fermentation medium: 0.2 % of yeast extract, peptone 0.3 %, KH2PO40.1 %,
(NH4)2 SO40.5 %, MgSO4·7H2O 0.05 %, sterilize under the conditions of 121 DEG C 20 min.
Embodiment 1
1) prepared by maize peel enzymolysis liquid: maize peel warp smashes it through 60 meshes, and purifying is added according to the weight ratio of 1:8
Water reconciles pH value to 6.0, and agitation revolution is 120 rpm/min, and hemicellulose degradation is added according to 6.0 % of maize peel dry
Complex enzyme, controlled enzymatic hydrolysis temperature are 80 DEG C, and then 30 h of enzymolysis time is filtered through plate and frame filter press, remove waste residue, obtain
Sugared weight percent concentration is 30 % in enzymolysis liquid；
Jerusalem artichoke juice preparation: fresh jerusalem artichoke cleaning weighing, 100 DEG C of 5 min enzyme deactivations of heating add 80 DEG C of appropriate amount of water to blend, double-deck
100 mesh filter cloth filters pressings, filter residue add appropriate hot water to repeat filters pressing, and mixed filtrate constant volume to every 100 g jerusalem artichoke goes out 100 mL juice, and 110
DEG C sterilizing 30 min；
2) Spawn incubation: barms and aspergillus niger strain are inoculated in the fermentor equipped with bacterium culture medium, 1:
0.6 air quantity, 200 rpm/min of revolving speed, cultivate 24 h, obtain bacteria culture fluid by 29 DEG C of cultivation temperature；
3) it is dense that the maize peel enzymolysis liquid of step 1) preparation and jerusalem artichoke juice fermented and cultured: are concentrated into weight percent respectively
Degree is 30%, as substrate, is added in the fermentor equipped with fermentation medium, and by the access step 2) preparation of 5 % inoculum concentrations
Bacteria culture fluid, 60 % of fermentation tank capacity, 400 r/min of mixing speed, ventilatory capacity are 2 L/min, are cultivated under the conditions of 29 DEG C
24 h, obtain fermentation culture；
4) fermentation culture for obtaining step 3) adds water and stirs, and 100 DEG C of 60 min of heating are cooled to 45 DEG C of centrifugations
Filtering, obtains level-one filtrate；The water centrifugal filtration again of centrifugation gained precipitating plus 2 times of amounts, obtains second level filtrate and precipitating；Gained
Two-stage precipitation adds the water centrifugal filtration of 2 times of amounts again, obtains three-level filtrate；Merge centrifugation gained filtrate three times, 3 % activity is added
Charcoal, 80 DEG C of 30 min of decoloration, is filtered while hot, by decoloration filtrate decompression concentration, health is made and substitutes sugared syrup.
Embodiment 2
1) prepared by maize peel enzymolysis liquid: maize peel warp smashes it through 60 meshes, and purifying is added according to the weight ratio of 1:3
Water reconciles pH value to 5.0, and agitation revolution is 50 rpm/min, and it is multiple that hemicellulose degradation is added according to 0.3 % of maize peel dry
Synthase, controlled enzymatic hydrolysis temperature are 45 DEG C, and then 8 h of enzymolysis time is filtered through plate and frame filter press, remove waste residue, obtained enzymatic hydrolysis
Sugared weight percent concentration is 10 % in liquid；
Jerusalem artichoke juice preparation: fresh jerusalem artichoke cleaning weighing, 100 DEG C of 5 min enzyme deactivations of heating add 80 DEG C of appropriate amount of water to blend, double-deck
100 mesh filter cloth filters pressings, filter residue add appropriate hot water to repeat filters pressing, and mixed filtrate constant volume to every 100 g jerusalem artichoke goes out 100 mL juice, and 110
DEG C sterilizing 30 min；
2) Spawn incubation: barms and aspergillus niger strain are inoculated in the fermentor equipped with bacterium culture medium, 1:
0.6 air quantity, 200 rpm/min of revolving speed, cultivate 24 h, obtain bacteria culture fluid by 29 DEG C of cultivation temperature；
3) it is dense that the maize peel enzymolysis liquid of step 1) preparation and jerusalem artichoke juice fermented and cultured: are concentrated into weight percent respectively
Degree is 20%, as substrate, is added in the fermentor equipped with fermentation medium, and by the access step 2) preparation of 5 % inoculum concentrations
Bacteria culture fluid, 60 % of fermentation tank capacity, 400 r/min of mixing speed, ventilatory capacity are 1.2 L/min, are trained under the conditions of 29 DEG C
24 h are supported, fermentation culture is obtained；
4) fermentation culture for obtaining step 3) adds water and stirs, and 100 DEG C of 30 min of heating are cooled to 45 DEG C of centrifugations
Filtering, obtains level-one filtrate；The water centrifugal filtration again of centrifugation gained precipitating plus 2 times of amounts, obtains second level filtrate and precipitating；Gained
Two-stage precipitation adds the water centrifugal filtration of 2 times of amounts again, obtains three-level filtrate；Merge centrifugation gained filtrate three times, 3 % activity is added
Charcoal, 80 DEG C of 30 min of decoloration, is filtered while hot, by decoloration filtrate decompression concentration, health is made and substitutes sugared syrup.
Embodiment 3
1) prepared by maize peel enzymolysis liquid: maize peel warp smashes it through 60 meshes, and purifying is added according to the weight ratio of 1:12
Water reconciles pH value to 5.5, and agitation revolution is 80 rpm/min, and it is multiple that hemicellulose degradation is added according to 3.0 % of maize peel dry
Synthase, controlled enzymatic hydrolysis temperature are 60 DEG C, and then 40 h of enzymolysis time is filtered through plate and frame filter press, remove waste residue, obtained enzyme
Solving weight percent concentration sugared in liquid is 20 %；
Jerusalem artichoke juice preparation: fresh jerusalem artichoke cleaning weighing, 100 DEG C of 5 min enzyme deactivations of heating add 80 DEG C of appropriate amount of water to blend, double-deck
100 mesh filter cloth filters pressings, filter residue add appropriate hot water to repeat filters pressing, and mixed filtrate constant volume to every 100 g jerusalem artichoke goes out 100 mL juice, and 110
DEG C sterilizing 30 min；
2) Spawn incubation: barms and aspergillus niger strain are inoculated in the fermentor equipped with bacterium culture medium, 1:
0.6 air quantity, 200 rpm/min of revolving speed, cultivate 24 h, obtain bacteria culture fluid by 29 DEG C of cultivation temperature；
3) it is dense that the maize peel enzymolysis liquid of step 1) preparation and jerusalem artichoke juice fermented and cultured: are concentrated into weight percent respectively
Degree is 40 %, as substrate, is added in the fermentor equipped with fermentation medium, and by the access step 2) preparation of 5 % inoculum concentrations
Bacteria culture fluid, 60 % of fermentation tank capacity, 400 r/min of mixing speed, ventilatory capacity is 1 L/min, is trained under the conditions of 29 DEG C
24 h are supported, fermentation culture is obtained；
4) fermentation culture for obtaining step 3) adds water and stirs, and 100 DEG C of 120 min of heating are cooled to 45 DEG C of centrifugations
Filtering, obtains level-one filtrate；The water centrifugal filtration again of centrifugation gained precipitating plus 2 times of amounts, obtains second level filtrate and precipitating；Gained
Two-stage precipitation adds the water centrifugal filtration of 2 times of amounts again, obtains three-level filtrate；Merge centrifugation gained filtrate three times, 3 % activity is added
Charcoal, 80 DEG C of 30 min of decoloration, is filtered while hot, by decoloration filtrate decompression concentration, health is made and substitutes sugared syrup.
Health substitution Icing Sugar can be made in health substitution syrup obtained through following steps:
Health obtained above is substituted sugared syrup to separate through centrifuge, is divided under the conditions of 50 DEG C or so, 6000 r/min
Solid phase and liquid phase are separated out, solid phase is utilized into drying machine with centrifugal spray, in the item that inlet air temperature is 160 DEG C, pressure is 0.5 MPa
Under part, carry out being spray-dried obtained health substitution Icing Sugar.
The constituent content of high effective liquid chromatography for measuring health substitution sugar
Chromatographic condition:
Chromatographic column: Ca2+Glycan analysis column (7.9mm × 300mm；10 μm of particle diameter)
Chromatographic column: Shimadzu shim-pack SCR-101C
Detector: RID-10A differential refraction detector
Mobile phase: triple distillation water, 15 min of excusing from death degassing
Flow velocity: 0.8 mL/min
Column temperature: (80.0 ± 1) DEG C
Sample volume: 20 μ L
The measurement of monosaccharide standard curve:
This experiment uses external standard method, control substance of plant drug is done with the sterling of component to be measured, with to be measured group in control substance of plant drug and sample
The response signal divided, which compares, to be quantified, and determines the standard curve of L-arabinose, fructose, xylose, glucose.
82 mL centrifuge tubes of preparation, precision weighs 10 mg of chromatographically pure L-arabinose and is placed in a centrifuge tube, and is added
1 mL of triple distillation water sufficiently dissolves, and obtains the standard solution that concentration is 10 mg/mL, therefrom draws 1 mL to second centrifugation
It is sufficiently dissolved in pipe, obtains the standard solution that concentration is 5 mg/mL, therefrom draw 1 mL into third centrifuge tube, it is such as such
Push away, to standard solution carry out gradient dilution, finally obtain concentration be 10,5,2.5,1.25,0.625,0.313,0.156,
The standard solution of 0.078 mg/mL.Fructose, xylose, glucose the same L-arabinose of sample handling characteristics.By four groups totally 32
A various concentration standard solution is measured according to the above chromatographic condition.Four standard curves and side are drawn according to measurement result
Journey.
Health made from embodiment 1 is substituted into sugared syrup according to the above chromatographic condition sample introduction, by the L-arabinose measured,
The peak area of fructose, xylose and glucose brings corresponding equation into, measures the concentration that health substitutes four groups of sugar in sugared syrup, Jin Erji
Calculate the component ratio of four groups of sugar.
According to above method measurement embodiment 2, the substitution sugar syrup of health made from embodiment 3, so calculate L- I
The component ratio of uncle's sugar, fructose, xylose and glucose.
As a result as follows:
| Sample component | L-arabinose | Fructose | Xylose | Glucose |
| Embodiment 1 | 60% | 50% | 3% | 2% |
| Embodiment 2 | 80% | 15% | 2% | 3% |
| Embodiment 3 | 70% | 20% | 5% | 5% |
The present invention substitutes sugar using health made from liquid state fermentation technology, and mellow in taste, sugariness is moderate, is not causing glycosuria
Also people are able to satisfy while the diseases such as disease, hyperglycemia, saprodontia to the needs of sweet taste.
100 people are chosen, are randomly divided into 10 groups, sugar are substituted using health made from embodiment 1-3 respectively, by normal table sugar
Usage amount is used for the production of beverage and cake, and trial test does not feel sweet taste deficiency it is demonstrated experimentally that health substitution of the invention is sugared,
And there is apparent mellow mouthfeel.
Claims (6)
1. a kind of health substitution sugar, which is characterized in that including following components several by mass percentage:
It comprises the following steps that:
1) prepared by maize peel enzymolysis liquid: maize peel warp smashes it through 60 meshes, and purifying is added according to the weight ratio of 1:3~1:12
Water reconciles pH value to 5.0~6.0, and agitation revolution is 50~120rpm, and half fiber is added according to the 0.3~6.0% of maize peel dry
Plain degradation complex enzyme is tieed up, controlled enzymatic hydrolysis temperature is 45~80 DEG C, then 8~40h of enzymolysis time is filtered through plate and frame filter press, gone
Except waste residue, sugared weight percent concentration is 10~30% in obtained enzymolysis liquid；
Jerusalem artichoke juice preparation: fresh jerusalem artichoke cleaning weighing, 100 DEG C of heating 5min enzyme deactivations add 80 DEG C of appropriate amount of water to blend, the double-deck 100 mesh filter
Cloth filters pressing, filter residue add appropriate hot water to repeat filters pressing, and mixed filtrate constant volume to every 100g jerusalem artichoke goes out 100mL juice, 110 DEG C of sterilizings
30min；
2) Spawn incubation: barms and aspergillus niger strain are inoculated in the fermentor equipped with bacterium culture medium, the wind of 1:0.6
Amount, revolving speed 200rpm, 29 DEG C of cultivation temperature, culture for 24 hours, obtains bacteria culture fluid；
3) fermented and cultured: it is 20 that the maize peel enzymolysis liquid of step 1) preparation and jerusalem artichoke juice are concentrated into weight percent concentration respectively
~40%, it as substrate, is added in the fermentor equipped with fermentation medium, and by the access step 2) preparation of 5% inoculum concentration
Bacteria culture fluid, fermentation tank capacity 60%, mixing speed 400rpm, ventilatory capacity are 1.2~2 L/min, are trained under the conditions of 29 DEG C
It supports for 24 hours, obtains fermentation culture；
4) fermentation culture for obtaining step 3) adds water and stirs, and 100 DEG C of 30~120min of heating are cooled to 45 DEG C and were centrifuged
Filter, obtains level-one filtrate；The water centrifugal filtration again of centrifugation gained precipitating plus 2 times of amounts, obtains second level filtrate and precipitating；Gained two
Grade precipitating adds the water centrifugal filtrations of 2 times of amounts again, obtains three-level filtrate；Merge centrifugation gained filtrate three times, 3% activity is added
Charcoal, 80 DEG C of decoloration 30min, is filtered while hot, by decoloration filtrate decompression concentration, health is made and substitutes sugared syrup.
2. a kind of health substitution sugar according to claim 1, which is characterized in that including the following of number by mass percentage
Component:
3. a kind of health substitution sugar according to claim 1, which is characterized in that including the following of number by mass percentage
Component:
4. a kind of health substitution sugar according to claim 1, which is characterized in that the component of the step 2) bacterium culture medium
For mass percent: yeast extract 1%, glucose 2%, peptone 2%, KH2PO40.5%, pH under the conditions of 121 DEG C naturally, sterilize
20min。
5. a kind of health substitution sugar according to claim 1, which is characterized in that the component of the step 3) fermentation medium
For mass percent: yeast extract 0.2%, peptone 0.3%, KH2PO40.1%, (NH4)2 SO40.5%, MgSO4·7H2O
0.05%, sterilize under the conditions of 121 DEG C 20min.
6. a kind of health substitution sugar according to claim 1, which is characterized in that health made from step 4) is substituted sugar sugar
Slurry is separated through centrifuge, and obtained solid phase is spray-dried, and health substitution Icing Sugar is made.
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| JP2005204537A (en) * | 2004-01-21 | 2005-08-04 | Unitika Ltd | L-arabinose-containing syrup |
| CN101715940A (en) * | 2009-11-30 | 2010-06-02 | 山东省鲁洲食品集团有限公司 | Xylose-arabinose syrup and method for preparing same |
| CN103948016A (en) * | 2014-05-19 | 2014-07-30 | 济南圣泉唐和唐生物科技有限公司 | Application of L-arabinose to coloring agent of fried minced fillet product |
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| JP2004261039A (en) * | 2003-02-28 | 2004-09-24 | Unitika Ltd | L-arabinose-containing crystalline powder sugar composition and method for producing the same |
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|---|---|---|---|---|
| JP2005204537A (en) * | 2004-01-21 | 2005-08-04 | Unitika Ltd | L-arabinose-containing syrup |
| CN101715940A (en) * | 2009-11-30 | 2010-06-02 | 山东省鲁洲食品集团有限公司 | Xylose-arabinose syrup and method for preparing same |
| CN103948016A (en) * | 2014-05-19 | 2014-07-30 | 济南圣泉唐和唐生物科技有限公司 | Application of L-arabinose to coloring agent of fried minced fillet product |
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