CN105815788A - Healthy substitute sugar and method for preparing healthy substitute sugar through liquid state fermentation technology - Google Patents
Healthy substitute sugar and method for preparing healthy substitute sugar through liquid state fermentation technology Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 52
- 230000004151 fermentation Effects 0.000 title claims abstract description 52
- 239000007788 liquid Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000005516 engineering process Methods 0.000 title claims abstract description 15
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- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 31
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- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of food, in particular to healthy substitute sugar and a method for preparing healthy substitute sugar through a liquid state fermentation technology.The healthy substitute sugar comprises, by mass, 40-90% of L- arabinose, 10-60% of fructose, 0-10% of xylose and 0-10% of glucose.The preparation method comprises the steps of preparation of corn bran enzymatic hydrolysate and jerusalem artichoke juice; culture of strains; fermentation and culture; centrifugal filtration, decoloration and vacuum concentration, so that the substitute sugar is obtained.Syrup of the substitute sugar is subjected to centrifugal separation and spray drying, and the substitute sugar can be made into substitute powdered sugar.The obtained healthy substitute sugar is applicable to people who are forbidden from eating cane sugar and people who love sweet food but are worried about that sweet food affects health.The required raw materials are wide in source and low in price, the physicochemical condition is easy to control, the production cycle is short, the healthy substitute sugar is stable in quality and high in yield, it is convenient to conduct industrialized production, and the preparation method is not controlled by seasons.
Description
Technical field
The present invention relates to food technology field, particularly relate to a kind of healthy replacement sugar and utilize liquid fermentation technology to prepare
The healthy method substituting sugar.
Background technology
Sugar is one of important substance of depending on for existence of the mankind, is one of big main nutrients of human body three, is human body heat energy
Main source.General " sugared " eaten typically refers to sucrose.Heat contained by every gram of sugar about 4 calories, if picked-up excess
And cannot consume in time, unnecessary heat will change into fat.Additionally, containing adding the food of sugar, beverage picked-up too much meeting
Make blood glucose rapid increase, cause insulin in blood to increase；Insulin can make health efficiently depot fat, causes fertilizer
Fat, the risk suffering from chronic disease such as cardiovascular disease can be increased.So some expert thinks: sugar is than cigarette and alcopop pair
The harm of human body also wants big.World Health Organization's investigation finds, sugar is taken in and heart disease, hypertension, blood vessel too much can be caused hard
Change disease and cerebral hemorrhage, diabetes etc..Addicted to the evil of sugar, more than smoking.Therefore, World Health Organization (WHO) proposes " whole world ring in nineteen ninety-five
Sugar " new slogan.Along with growth in the living standard, people are to adding the high heating value such as sucrose, glucose and having certain side effect
Sugar is not welcome.And the use of sucrose is long-standing, the people especially children peculiar sense of taste to sucrose sweet taste
Dependency is difficult to shake.The most both keep people's habitual sucrose taste, benefit from again functional sugar low sugar, nutrition etc.
New concept of health, is also a problem of puzzled food service industry.
L-arabinose (L-arabinose) is also known as pectose, it is possible to the absorption of suppression sucrose, adds 3.5 in sucrose
The ratio of %, it is possible to the absorption of suppression human body 60 ~ 70 % sucrose, therefore life-time service can control blood glucose rising can suppress fertile
Fat, prevent the disease relevant to hyperglycemia；Constipation, promotes bifidobacterium growth；Change the merit of skeletal muscle fiber composition
Effect, may be had to the glycoxidative growth affecting abdominal adipose tissue, the change of this Muscle fiber density by domination glycolysis
Improve the effect of type 2 diabetes mellitus.
Fructose is a kind of refinement from various fruit and corn, All Pure Nature, new saccharide that sweet taste is strong, because not being easily caused high blood
Sugar, is not easy to occur athero to get fat, more will not produce dental caries, and be recognized by more people.The sugariness of fructose is
1.8 times of sucrose, are the sugar that in all natural sugar, sugariness is the highest, so under same sweetness standard, the intake of fructose is only
Half for sucrose.And with synthesis for compared with sugar, fructose is the most more reliable on trophism with safety.It can be said that fructose
Be at present the most in the world known to one of the safest, the most healthy sugar.
Xylose is a component of xylan, is widely present in plant.The most digested absorption of xylose, does not has energy value,
Can meet to greatest extent and like that eating sweets worries again the demand of the person of getting fat；Edible xylose can improve the microbial environment of human body, carries
The immunocompetence of high body；Xylose is not utilized by Institute of Micro-biology in oral cavity, possesses the part physiological function of dietary fiber, it is possible to decrease
Serum cholesterol, prevention intestinal cancer etc.；Xylose is fine with the compatibility of food, adds a small amount of xylose, just can embody very in food
Good health-care effect；Xylose is taken in calcium simultaneously, can improve human body to the absorbance of calcium and retention rate, moreover it is possible to prevent constipation.
Along with growth in the living standard, the health that people are increasingly careful in one's diet, thus develop a kind of novel, can meet
People's mouthfeel is difficult to again cause the sweeting agent of multiple disease extremely urgent.At present, major part uses functional sugar alcohol such as xylitol, wheat
The part substituting saccharoses such as bud sugar alcohol, but owing to people are when functional sugar alcohol such as edible xylitol, maltose alcohols, it may appear that diarrhoea,
The adverse consequences of abdominal distention, therefore its amount and edible crowd are partly limited.Prior art is mostly by melted or vacuum
Several sugar are mixed with functional sugar by the methods such as concentration, but this kind of method cost of material is high, is difficult to expanding production；Utilize plant
Raw material is prepared arabinose, fructose etc. and is typically required the chemical methodes such as acid hydrolysis, not only complex process in process of production, and
There is the potential risk introducing chemical composition.
Summary of the invention
It is an object of the invention to, it is provided that a kind of health substitutes sugar and utilizes liquid fermentation technology preparation health replacement sugar
Method.The method is that the method preparation health utilizing strain liquid submerged fermentation substitutes sugar, compared with common sucrose, mouthfeel alcohol
Thickness, sugariness is moderate, will not produce some such as common diseases such as diabetes, cardiovascular diseases because excess is edible, it is adaptable to prohibit
Sucrose crowd and vast sweet tooth and be afraid of unhealthful crowd eat, thoroughly compensate for what people were brought by edible sucrose
Harm.Raw materials used in preparation utilize raw material for secondary, simple and easy to get, environmental protection；Instrument floor space is little, fermentation efficiency
Height, easy to operate, it is substantially reduced in labor intensity, and operating process and does not introduce dangerous material, reduce energy resource consumption, low cost
Honest and clean, it is easy to mechanization, automated production.
To this end, technical scheme is as follows:
A kind of healthy replacement sugar, the following component including counting by mass percentage:
L-arabinose 40 ~ 90 %
Fructose 10 ~ 60 %
Xylose 0 ~ 10 %
Glucose 0 ~ 10 %.
Above-mentioned a kind of healthy replacement sugar, its component counted by mass percentage is preferably:
L-arabinose 50 ~ 70 %
Fructose 20 ~ 40 %
Xylose 0 ~ 5 %
Glucose 0 ~ 5 %.
Above-mentioned a kind of healthy substitute sugar, its component counted by mass percentage more preferably:
L-arabinose 60 %
Fructose 35 %
Xylose 3 %
Glucose 2 %.
A kind of method utilizing liquid fermentation technology to prepare above-mentioned healthy replacement sugar, comprises the following steps:
1) prepared by skin of Semen Maydis enzymolysis solution: skin of Semen Maydis crosses 60 mesh sieves after crushed, adds purification according to the part by weight of 1:3 ~ 1:12
Water, conciliation pH value is to 5.0 ~ 6.0, and agitation revolution is 50 ~ 120 rpm/min, adds half according to 0.3 ~ 6.0 % of skin of Semen Maydis dry
Cellulose degradation compound enzyme, controlled enzymatic hydrolysis temperature is 45 ~ 80 DEG C, enzymolysis time 8 ~ 40 h, then filters through filter press,
Removing waste residue, in the enzymolysis solution obtained, the weight percent concentration of sugar is 10 ~ 30 %；
Prepared by jerusalem artichoke juice: fresh Jerusalem artichoke is cleaned and weighs, 100 DEG C of heating 5 min enzyme denaturing, adds 80 DEG C of water and blends in right amount, double-deck 100 mesh
Filter cloth filter pressing, filtering residue adds appropriate hot water and repeats filter pressing, and mixing filtrate constant volume goes out 100 mL juice to every 100 g Jerusalem artichokes, and 110 DEG C go out
Bacterium 30 min；
2) spawn culture: barms and aspergillus niger strain are inoculated in equipped with in the fermentation tank of bacterium culture medium, 1:0.6's
Air quantity, rotating speed 200 rpm/min, cultivation temperature 29 DEG C, cultivate 24 h, obtain bacteria culture fluid；
3) fermentation culture: by step 1) the skin of Semen Maydis enzymolysis solution prepared and jerusalem artichoke juice be concentrated into weight percent concentration respectively and be
20 ~ 40 %, as substrate, are equipped with in the fermentation tank of fermentation medium, and access step 2 by 5 % inoculum concentrations) prepare
Bacteria culture fluid, fermentation tank liquid amount 60 %, mixing speed 400 r/min, ventilation is 1.2 ~ 2 L/min, under the conditions of 29 DEG C
Cultivate 24 h, obtain fermentation culture；
4) by step 3) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 ~ 120 min, and it is cooled to 45 DEG C and is centrifuged
Filter, obtain one-level filtrate；The water recentrifuge that centrifugal gained precipitation adds 2 times amount filters, and obtains two grades of filtrates and precipitation；Gained
Two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three grades of filtrates；Merge three centrifugal gained filtrates, add 3 % activity
Charcoal, 80 DEG C of decolouring 30 min, filtered while hot, will decolour filtrate reduced in volume, prepare health and substitute sugar syrup.
The healthy sugar syrup machine by centrifugation that substitutes step 4) prepared separates, and the solid phase obtained is spray-dried, and prepares
Healthy replacement Icing Sugar.
Step 2) component of described bacterium culture medium is (mass percent): yeast extract 1 %, glucose 2 %, peptone 2
%, KH2PO40.5 %, pH are natural, sterilizing 20 min under the conditions of 121 DEG C.
Step 3) component of described fermentation medium is (mass percent): yeast extract 0.2 %, peptone 0.3 %,
KH2PO40.1 %, (NH4)2 SO40.5 %, MgSO4·7H2O 0.05 %, sterilizing 20 min under the conditions of 121 DEG C.
The invention have the advantage that
1) utilize L-arabinose can optionally suppress the activity of saccharase in small intestinal, thus reach the suppression suction to sucrose
Receive, and then the disease that prevention is relevant to hyperglycemia, it is adaptable to prohibit sucrose crowd and vast sweet tooth and be afraid of unhealthful
Crowd eats.Thoroughly compensate for the harm that people are brought by edible sucrose；
2) the healthy fructose substituted in sugar is not easily caused hyperglycemia, is not easy to occur athero to get fat, more will not produce dental caries
Tooth, a small amount of interpolation can meet people and suit one's taste demand；
3) in preparation, needed raw material wide material sources are cheap, and the nutrition distribution pattern of fluid medium is uniform, beneficially mushroom
Being fully contacted and absorb of trophosome；
4) in incubation, physico chemical factor is easily controllable, production technology specification, with short production cycle, constant product quality, produces
Amount height, low to Preparation equipment and site requirements, it is simple to industrialized production, and not by control in season.
Detailed description of the invention
The detailed description of the invention of form by the following examples, is described in further detail the foregoing of the present invention.But
This should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to following example.All realized based on foregoing of the present invention
Technology belong to the scope of the present invention.
Barms (torulopsis, numbering: ACCC20343), aspergillus niger strain (numbering: CICC41529) be purchased from micro-
Biological inoculum preservation center；
Bacterium culture medium component (mass percent): yeast extract 1 %, glucose 2 %, peptone 2 %, KH2PO40.5 %, pH
Natural, sterilizing 20 min under the conditions of 121 DEG C；
The component (mass percent) of fermentation medium: yeast extract 0.2 %, peptone 0.3 %, KH2PO40.1 %, (NH4)2
SO40.5 %, MgSO4·7H2O 0.05 %, sterilizing 20 min under the conditions of 121 DEG C.
Embodiment 1
1) prepared by skin of Semen Maydis enzymolysis solution: skin of Semen Maydis crosses 60 mesh sieves after crushed, adds purified water according to the part by weight of 1:8, adjusts
Solution pH value is to 6.0, and agitation revolution is 120 rpm/min, adds hemicellulose degradation according to 6.0 % of skin of Semen Maydis dry and is combined
Enzyme, controlled enzymatic hydrolysis temperature is 80 DEG C, enzymolysis time 30 h, then filters through filter press, removes waste residue, the enzymolysis obtained
In liquid, the weight percent concentration of sugar is 30 %；
Prepared by jerusalem artichoke juice: fresh Jerusalem artichoke is cleaned and weighs, 100 DEG C of heating 5 min enzyme denaturing, adds 80 DEG C of water and blends in right amount, double-deck 100 mesh
Filter cloth filter pressing, filtering residue adds appropriate hot water and repeats filter pressing, and mixing filtrate constant volume goes out 100 mL juice to every 100 g Jerusalem artichokes, and 110 DEG C go out
Bacterium 30 min；
2) spawn culture: barms and aspergillus niger strain are inoculated in equipped with in the fermentation tank of bacterium culture medium, 1:0.6's
Air quantity, rotating speed 200 rpm/min, cultivation temperature 29 DEG C, cultivate 24 h, obtain bacteria culture fluid；
3) fermentation culture: by step 1) the skin of Semen Maydis enzymolysis solution prepared and jerusalem artichoke juice be concentrated into weight percent concentration respectively and be
30%, as substrate, be equipped with in the fermentation tank of fermentation medium, and access steps 2 by 5 % inoculum concentrations) strain prepared
Culture fluid, fermentation tank liquid amount 60 %, mixing speed 400 r/min, ventilation is 2 L/min, cultivates 24 under the conditions of 29 DEG C
H, obtains fermentation culture；
4) by step 3) fermentation culture that obtains adds water stirring, 100 DEG C of heating 60 min, is cooled to 45 DEG C of centrifugal filtrations,
Obtain one-level filtrate；The water recentrifuge that centrifugal gained precipitation adds 2 times amount filters, and obtains two grades of filtrates and precipitation；Gained two grades
Precipitation adds the water centrifugal filtration of 2 times amount again, obtains three grades of filtrates；Merge three centrifugal gained filtrates, add 3 % activated carbons,
80 DEG C of decolouring 30 min, filtered while hot, will decolour filtrate reduced in volume, prepare health and substitute sugar syrup.
Embodiment 2
1) prepared by skin of Semen Maydis enzymolysis solution: skin of Semen Maydis crosses 60 mesh sieves after crushed, adds purified water according to the part by weight of 1:3, adjusts
Solution pH value is to 5.0, and agitation revolution is 50 rpm/min, adds hemicellulose degradation compound enzyme according to 0.3 % of skin of Semen Maydis dry,
Controlled enzymatic hydrolysis temperature is 45 DEG C, enzymolysis time 8 h, then filters through filter press, removes waste residue, in the enzymolysis solution obtained
The weight percent concentration of sugar is 10 %；
Prepared by jerusalem artichoke juice: fresh Jerusalem artichoke is cleaned and weighs, 100 DEG C of heating 5 min enzyme denaturing, adds 80 DEG C of water and blends in right amount, double-deck 100 mesh
Filter cloth filter pressing, filtering residue adds appropriate hot water and repeats filter pressing, and mixing filtrate constant volume goes out 100 mL juice to every 100 g Jerusalem artichokes, and 110 DEG C go out
Bacterium 30 min；
2) spawn culture: barms and aspergillus niger strain are inoculated in equipped with in the fermentation tank of bacterium culture medium, 1:0.6's
Air quantity, rotating speed 200 rpm/min, cultivation temperature 29 DEG C, cultivate 24 h, obtain bacteria culture fluid；
3) fermentation culture: by step 1) the skin of Semen Maydis enzymolysis solution prepared and jerusalem artichoke juice be concentrated into weight percent concentration respectively and be
20%, as substrate, be equipped with in the fermentation tank of fermentation medium, and access steps 2 by 5 % inoculum concentrations) strain prepared
Culture fluid, fermentation tank liquid amount 60 %, mixing speed 400 r/min, ventilation is 1.2 L/min, cultivates 24 under the conditions of 29 DEG C
H, obtains fermentation culture；
4) by step 3) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 min, is cooled to 45 DEG C of centrifugal filtrations,
Obtain one-level filtrate；The water recentrifuge that centrifugal gained precipitation adds 2 times amount filters, and obtains two grades of filtrates and precipitation；Gained two grades
Precipitation adds the water centrifugal filtration of 2 times amount again, obtains three grades of filtrates；Merge three centrifugal gained filtrates, add 3 % activated carbons,
80 DEG C of decolouring 30 min, filtered while hot, will decolour filtrate reduced in volume, prepare health and substitute sugar syrup.
Embodiment 3
1) prepared by skin of Semen Maydis enzymolysis solution: skin of Semen Maydis crosses 60 mesh sieves after crushed, adds purified water according to the part by weight of 1:12,
Conciliation pH value is to 5.5, and agitation revolution is 80 rpm/min, adds hemicellulose degradation according to 3.0 % of skin of Semen Maydis dry and is combined
Enzyme, controlled enzymatic hydrolysis temperature is 60 DEG C, enzymolysis time 40 h, then filters through filter press, removes waste residue, the enzymolysis obtained
In liquid, the weight percent concentration of sugar is 20 %；
Prepared by jerusalem artichoke juice: fresh Jerusalem artichoke is cleaned and weighs, 100 DEG C of heating 5 min enzyme denaturing, adds 80 DEG C of water and blends in right amount, double-deck 100 mesh
Filter cloth filter pressing, filtering residue adds appropriate hot water and repeats filter pressing, and mixing filtrate constant volume goes out 100 mL juice to every 100 g Jerusalem artichokes, and 110 DEG C go out
Bacterium 30 min；
2) spawn culture: barms and aspergillus niger strain are inoculated in equipped with in the fermentation tank of bacterium culture medium, 1:0.6's
Air quantity, rotating speed 200 rpm/min, cultivation temperature 29 DEG C, cultivate 24 h, obtain bacteria culture fluid；
3) fermentation culture: by step 1) the skin of Semen Maydis enzymolysis solution prepared and jerusalem artichoke juice be concentrated into weight percent concentration respectively and be
40 %, as substrate, are equipped with in the fermentation tank of fermentation medium, and access steps 2 by 5 % inoculum concentrations) bacterium prepared
Planting culture fluid, fermentation tank liquid amount 60 %, mixing speed 400 r/min, ventilation is 1 L/min, cultivates 24 under the conditions of 29 DEG C
H, obtains fermentation culture；
4) by step 3) fermentation culture that obtains adds water stirring, 100 DEG C of heating 120 min, and it is cooled to 45 DEG C and was centrifuged
Filter, obtains one-level filtrate；The water recentrifuge that centrifugal gained precipitation adds 2 times amount filters, and obtains two grades of filtrates and precipitation；Gained two
Level precipitation adds the water centrifugal filtration of 2 times amount again, obtains three grades of filtrates；Merge three centrifugal gained filtrates, add 3 % activity
Charcoal, 80 DEG C of decolouring 30 min, filtered while hot, will decolour filtrate reduced in volume, prepare health and substitute sugar syrup.
The healthy syrup that substitutes prepared can be through the prepared healthy Icing Sugar that substitutes of following steps:
The above-mentioned prepared healthy sugar syrup machine by centrifugation that substitutes is separated, about 50 DEG C, isolate under the conditions of 6000 r/min
Solid phase and liquid phase, utilize drying machine with centrifugal spray by solid phase, inlet temperature be 160 DEG C, under conditions of pressure is 0.5 MPa,
Carry out being spray-dried and prepare health replacement Icing Sugar.
High effective liquid chromatography for measuring health substitutes the constituent content of sugar
Chromatographic condition:
Chromatographic column: Ca2+Glycan analysis post (7.9mm × 300mm；Particle diameter 10 μm)
Chromatographic column: Shimadzu shim-pack SCR-101C
Detector: RID-10A differential refraction detector
Flowing phase: triple distillation water, excusing from death degassing 15 min
Flow velocity: 0.8 mL/min
Column temperature: (80.0 ± 1) DEG C
Sample size: 20 μ L
The mensuration of monosaccharide standard curve:
This experiment uses external standard method, does control substance of plant drug by the sterling of component to be measured, with component to be measured in control substance of plant drug and sample
Response signal compares and carries out quantitatively, determining the standard curve of L-arabinose, fructose, xylose, glucose.
Preparing 82 mL centrifuge tubes, precision weighs chromatographically pure L-arabinose 10 mg and is placed in a centrifuge tube, and adds
Triple distillation water 1 mL fully dissolves, and obtains the standard solution that concentration is 10 mg/mL, therefrom draws 1 mL to second and is centrifuged
Pipe fully dissolves, obtains the standard solution that concentration is 5 mg/mL, therefrom draw in 1 mL to the 3rd centrifuge tube, such as this type of
Push away, standard solution carried out gradient dilution, finally give concentration be 10,5,2.5,1.25,0.625,0.313,0.156,
The standard solution of 0.078 mg/mL.Fructose, xylose, the same L-arabinose of sample handling characteristics of glucose.By four groups totally 32
Individual variable concentrations standard solution is measured according to above chromatographic condition.Four standard curves and side are drawn according to measurement result
Journey.
The healthy sugar syrup that substitutes that embodiment 1 is prepared according to above chromatographic condition sample introduction, by the L-arabinose recorded,
The peak area of fructose, xylose and glucose brings corresponding equation into, records the concentration of four groups of sugar, Jin Erji in healthy replacement sugar syrup
Calculate the component ratio of four groups of sugar.
Measure embodiment 2 according to above method, the healthy sugar syrup that substitutes that embodiment 3 prepares, and then calculate L-I
Uncle's sugar, fructose, xylose and the component ratio of glucose.
Result is as follows:
| Sample component | L-arabinose | Fructose | Xylose | Glucose |
| Embodiment 1 | 60% | 50% | 3% | 2% |
| Embodiment 2 | 80% | 15% | 2% | 3% |
| Embodiment 3 | 70% | 20% | 5% | 5% |
What the present invention utilized that liquid fermentation technology prepares healthy substitutes sugar, mellow in taste, and sugariness is moderate, do not cause diabetes,
Also people's needs to sweet taste can be met while the disease such as hyperglycemia, dental caries.
Choose 100 people, be randomly divided into 10 groups, use the healthy replacement sugar that embodiment 1-3 prepares respectively, by normal table sugar
Usage amount is used for beverage and the making of cake, tastes it is demonstrated experimentally that the healthy sugar that substitutes of the present invention does not feel that sweet taste is not enough,
And there is obvious mellow mouthfeel.
Claims (7)
1. one kind healthy substitutes sugar, it is characterised in that include the following component counted by mass percentage:
L-arabinose 40 ~ 90 %
Fructose 10 ~ 60 %
Xylose 0 ~ 10 %
Glucose 0 ~ 10 %.
The most according to claim 1 a kind of healthy substitute sugar, it is characterised in that include counting by mass percentage is following
Component:
L-arabinose 50 ~ 70 %
Fructose 20 ~ 40 %
Xylose 0 ~ 5 %
Glucose 0 ~ 5 %.
The most according to claim 1 a kind of healthy substitute sugar, it is characterised in that include counting by mass percentage is following
Component:
L-arabinose 60 %
Fructose 35 %
Xylose 3 %
Glucose 2 %.
4. one kind utilizes liquid fermentation technology to prepare the healthy method substituting sugar described in claim 1 ~ 3, it is characterised in that include
Following steps:
1) prepared by skin of Semen Maydis enzymolysis solution: skin of Semen Maydis crosses 60 mesh sieves after crushed, adds purification according to the part by weight of 1:3 ~ 1:12
Water, conciliation pH value is to 5.0 ~ 6.0, and agitation revolution is 50 ~ 120 rpm/min, adds half according to 0.3 ~ 6.0 % of skin of Semen Maydis dry
Cellulose degradation compound enzyme, controlled enzymatic hydrolysis temperature is 45 ~ 80 DEG C, enzymolysis time 8 ~ 40 h, then filters through filter press,
Removing waste residue, in the enzymolysis solution obtained, the weight percent concentration of sugar is 10 ~ 30 %；
Prepared by jerusalem artichoke juice: fresh Jerusalem artichoke is cleaned and weighs, 100 DEG C of heating 5 min enzyme denaturing, adds 80 DEG C of water and blends in right amount, double-deck 100 mesh
Filter cloth filter pressing, filtering residue adds appropriate hot water and repeats filter pressing, and mixing filtrate constant volume goes out 100 mL juice to every 100 g Jerusalem artichokes, and 110 DEG C go out
Bacterium 30 min；
2) spawn culture: barms and aspergillus niger strain are inoculated in equipped with in the fermentation tank of bacterium culture medium, 1:0.6's
Air quantity, rotating speed 200 rpm/min, cultivation temperature 29 DEG C, cultivate 24 h, obtain bacteria culture fluid；
3) fermentation culture: by step 1) the skin of Semen Maydis enzymolysis solution prepared and jerusalem artichoke juice be concentrated into weight percent concentration respectively and be
20 ~ 40%, as substrate, be equipped with in the fermentation tank of fermentation medium, and access step 2 by 5 % inoculum concentrations) prepare
Bacteria culture fluid, fermentation tank liquid amount 60 %, mixing speed 400 r/min, ventilation is 1.2 ~ 2 L/min, under the conditions of 29 DEG C
Cultivate 24 h, obtain fermentation culture；
4) by step 3) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 ~ 120 min, and it is cooled to 45 DEG C and is centrifuged
Filter, obtain one-level filtrate；The water recentrifuge that centrifugal gained precipitation adds 2 times amount filters, and obtains two grades of filtrates and precipitation；Gained
Two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three grades of filtrates；Merge three centrifugal gained filtrates, add 3 % activity
Charcoal, 80 DEG C of decolouring 30 min, filtered while hot, will decolour filtrate reduced in volume, prepare health and substitute sugar syrup.
A kind of method utilizing liquid fermentation technology preparation health to substitute sugar the most according to claim 4, it is characterised in that
Step 2) component of described bacterium culture medium is (mass percent): yeast extract 1 %, glucose 2 %, peptone 2 %, KH2PO4
0.5 %, pH are natural, sterilizing 20 min under the conditions of 121 DEG C.
A kind of method utilizing liquid fermentation technology preparation health to substitute sugar the most according to claim 4, it is characterised in that
Step 3) component of described fermentation medium is (mass percent): yeast extract 0.2 %, peptone 0.3 %, KH2PO40.1 %,
(NH4)2 SO40.5 %, MgSO4·7H2O 0.05 %, sterilizing 20 min under the conditions of 121 DEG C.
A kind of method utilizing liquid fermentation technology preparation health to substitute sugar the most according to claim 4, it is characterised in that
The healthy sugar syrup machine by centrifugation that substitutes step 4) prepared separates, and the solid phase obtained is spray-dried, and prepares health and substitutes
Icing Sugar.
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