CN106387917A - Preparation method of antialcoholism product - Google Patents
Preparation method of antialcoholism product Download PDFInfo
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- CN106387917A CN106387917A CN201610823330.XA CN201610823330A CN106387917A CN 106387917 A CN106387917 A CN 106387917A CN 201610823330 A CN201610823330 A CN 201610823330A CN 106387917 A CN106387917 A CN 106387917A
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Abstract
The invention relates to the technical field of foods, in particular to a preparation method of an antialcoholism product. The antialcoholism product comprises alcohol dehydrogenase and aldehyde dehydrogenase obtained through crushing saccharomyces cerevisiae cells, as well as L-arabinose obtained through fermentation of corn husk enzymatic hydrolysate. The product prepared by the method disclosed by the invention can be made into a product in any forms accepted by bromatology and health-care food science, and the made antialcoholism product has obvious effect of neutralizing the effect of alcoholic drinks.
Description
Technical field
The present invention relates to food technology field, more particularly to a kind of preparation method of disintoxicating product.
Background technology
China is a country having long spirits culture, defines its unique style in very long evolution,
In occupation of unique status, almost penetrate into the every field of social life.The life that wine gives people the people adds abundant color,
A lot of people are fond of drinking, or inevitably drink because of need of work.Responsible drinking has certain benefit to human body, not only may be used
To rouse oneself mood, and the blood circulation of human body can be promoted.Once but excessive consumption of alcohol, will cause such as Nausea and vomiting,
Failure of memory, absent minded, mobility impairment and emotional instability etc., even lead to because of respiratory paralysis when serious
Dead.Had data to show, cancer of the esophagus, onset of liver cancer rate rising also with drink relevant.In recent years, fatty liver, alcoholic liver,
The incidence of disease of other liver diseases such as medicine liver is rising year by year, becomes the serious class disease threatening our people's life and health.
And the evident characteristic that AML is different from other property hepatopathys is, AML is long-term excessive consumption of alcohol and is formed.
For a long time, the people to release gentle brought discomfort of relieving the effect of alcohol using various methods.The used articles for use five that relieve the effect of alcohol
Hua Bamen.Disintoxicating product is broadly divided into two classes at present, and the first kind is protein peptides disintoxicating product, and the drunk composition of its suppression is with spy
After different protein breakdown ferment treatment protein, it is cut into the protein peptides of segment piece.Dense by improving alanine and leucine in blood
Degree produces stable NAD+Thus effectively reducing Ethanol concentration in rat blood.But neither slow down ethanol discharging from stomach, it is not to subtract
Slow absorption.Equations of The Second Kind is based on the work effectively facilitating the ethanol degradation enzyme such as P-450 enzyme alcohol dehydrogenase and acetaldehyde dehydrogenase in liver
Power, this kind of product, how with Chinese herbal medicine extract as functional component, is drunk when people drink, with acceleration of alcohol metabolism in vivo.Mesh
Front domestic and international beverage for dispelling alcoholic intoxication mostly is simple excited type, enters body and produces class matter hormone effect, thus " neutralization " acetaldehyde is to human body
The inhibitory action of central nervous system, plays effect of sobering up, and only " takes stopgap measures ".Most commercially available prod is merely able to play liver protection
Effect, can not relieve the effect of alcohol, and be drunk after the liver protecting that carries out it is impossible to reduce drunk bring painful, uncomfortable, and its
Being divided into chemical synthesis material " sobering up " function, relevant expert not thinks and should advocate use more.
Mainly by two kinds of internal enzymes, one kind is alcohol dehydrogenase for metabolism in human body for the ethanol（Alcohol
Dehyfrogenase, ADH）, another kind is acetaldehyde dehydrogenase（Aldehyde Dehydrogenase, ALDH）.Ethanol is in human body
Interior metabolic response equation is as follows：
In above-mentioned reaction, ADH catalysis makes ethanol be converted into acetaldehyde.Acetaldehyde is further converted to second under the catalytic action of ALDH
Acid, is finally decomposed to carbon dioxide and water.It is usually present alcohol dehydrogenase in human body, and quantity is of substantially equal.But lack
The people of acetaldehyde dehydrogenase is relatively more.ALDH lacks, and so that ethanol can not be completely broken down as carbon dioxide, but the shape with acetaldehyde
Formula continues to stay in the body.Numerous studies show, bulk deposition is drunk most important reason to acetaldehyde in vivo both at home and abroad.Because losing
Pass and individual difference, Different Individual, the speed sum measurer of not agnate internal production ALDH have significant otherness, also just make
Become the difference of capacity for liquor size.But, the even internal higher crowd of ALDH level, if heavy drinking at short notice,
Internal ALDH still cannot decompose rapidly the acetaldehyde of accumulation.Anti-intoxication best bet be make introducing ethanol first rapid in stomach
Decompose, reduce ethanol as far as possible and enter blood circulation system and enter liver, to mitigate burden of liver.How just to enable ethanol in stomach
Inside first obtain this target biodegradable, key technology is that searching out one kind quickly can be converted into acetic acid by acetaldehyde, then decomposes
Ethanol degradation enzyme for carbon dioxide and water or converting Enzyme.
At present ALDH separation and Extraction mainly from the liver of animal, pancreas or stem cell mitochondria, but its resource-constrained
And expensive, it is difficult to large-scale production.
Develop effective antialcoholic drugs with biological method and there are great social effect and wide market prospects.Great majority
Relieve the effect of alcohol product mainly with plant for raw material be obtained, relatively costly, if can directly from microorganism extract acetaldehyde dehydrogenase pharmacy or
Directly cost will be substantially reduced using microbiological pharmacy.
Saccharomyces cerevisiae（Saccharomycescerevisiae）It is the bacterial strain of high yield ADH and ALDH, ADH and ALDH can drop
Solution ethanol.
L-arabinose can effectively reduce internal organs Fat Accumulation, slows down body weight increase；Reduce serum triglyceride, high
Density lipoprotein, improves high fat of blood, and suppression blood pressure raises；Improve intestinal microecology, promote bifidobacterium growth；L-arabinose
The metabolic process to alcohol for the alcohol user can be accelerated, effectively improves by improving the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase
Metabolic capability to alcohol, alleviates the injury that alcohol is caused to human body in human body due to savings time length.L- is Arabic simultaneously
Sugar can protect stomach lining, reduces the damage to stomach lining for the alcohol.Due to the presence of L-arabinose, ethanol and acetaldehyde metabolism add
Fast so that alcohol amount retained in human body is relatively fewer, thus also make people occur dizzy, blush, heartbeat is overrun even expression
Unclear phenomenon reduces, and has obvious anti-intoxication alcohol neutralizing effect.
Maize peel is the Main By product of starch industry, and maize peel accounts for 9 % ~ 13 % of iblet weight, is that corn forms sediment
Principal by product in powder processing industry.Wherein contain abundant araboxylan, it is permissible that araboxylan passes through enzyme hydrolysis
Obtain L-arabinose.
Content of the invention
It is an object of the invention to, a kind of preparation method of disintoxicating product is provided.The method is to be determined by many experiments
In preparation process, every optimized parameter, takes off using by obtaining ethanol by cell breaking technology after saccharomyces cerevisiae bacterial classification Amplification Culture
Hydrogen enzyme and acetaldehyde dehydrogenase, meanwhile, through the prepared L-arabinose of fermentation by saccharomyces cerevisiae after maize peel enzymolysis, alcohol dehydrogenase,
Acetaldehyde dehydrogenase and the common centrifugal filtration of L-arabinose are obtained disintoxicating product, make bromatology, health care by adding auxiliary material
The acceptable any type of product of bromatology, including tablet, capsule, granule, oral liquid or beverage etc..Relieve the effect of alcohol with commercially available
Product is compared, and the present invention is obtained rather than chemical synthesis material for fermentable, not only " takes stopgap measures " but also " effecting a permanent cure ", alcohol is in stomach
Intracellular metabolite and do not enter liver so protect liver, mitigate burden of liver；L-arabinose therein not only improves alcohol dehydrogenase
Enzyme and the activity of acetaldehyde dehydrogenase, protect stomach lining, reduce the damage to stomach lining for the alcohol, and be obtained disintoxicating product because
The presence mellow in taste of L-arabinose, sugariness is moderate, is also simultaneously suitable for prohibiting sucrose with diabetes, cardiovascular disease etc.
Crowd uses.In preparation, maize peel used is the accessory substance of starch industry, is secondary utilization raw material, simple and easy to get, environmental protection；
Instrument floor space is little, and fermentation efficiency is high, easy to operate, substantially reduces labour intensity, and does not introduce dangerous in operating process
Material, reduces energy resource consumption, with low cost it is easy to mechanization, automated production.
For this reason, technical scheme is as follows：
A kind of preparation method of disintoxicating product, comprises the following steps：
1) Spawn incubation：Saccharomyces cerevisiae bacterial classification is inoculated in equipped with the fermentation tank of bacterium culture medium, liquid amount 10 %, inoculation
Measure 7 %, air quantity is 1：0.6, pH 7, rotating speed 200 rpm/min, 28 ~ 30 DEG C of cultivation temperature, cultivate 16 h, obtain bacterial classification training
Nutrient solution；
2) by step 1) centrifugation of the bacteria culture fluid prepared, abandoning supernatant is precipitated, with 0.066 mol/L phosphoric acid hydrogen two
Sodium buffer solution 2 ~ 3 times, retains somatic cells, adds the Tris-cl containing 5 % 50 mM（pH 8.0）、0.2 % 1 mM
EDTA, the buffer solution of the NaCl of 2 % 100 mM, carry out clasmatosis with high pressure crusher, obtain containing alcohol dehydrogenase
Solution with acetaldehyde dehydrogenase；
3) maize peel enzymolysis liquid preparation：Maize peel crosses 60 mesh sieves after crushed, according to 1：3~1：12 part by weight adds purifying
Water, conciliation pH value to 5.0 ~ 6.0, agitation revolution is 50 ~ 120 rpm/min, and 0.3 ~ 6.0 % according to maize peel dry adds half
Cellulose degradation complex enzyme, controlled enzymatic hydrolysis temperature is 45 ~ 80 DEG C, enzymolysis time 8 ~ 40 h, then filters through plate and frame filter press,
Remove waste residue, in the enzymolysis liquid obtaining, the weight percent concentration of sugar is 10 ~ 30 %；
4) fermented and cultured：By step 3) to be concentrated into weight percent concentration be 20 ~ 40 % for the maize peel enzymolysis liquid prepared, with this
For substrate, add equipped with the fermentation tank of fermentation medium, and by 5 % inoculum concentrations access steps 1) bacteria culture fluid prepared,
Fermentation tank liquid amount 60 %, mixing speed 400 r/min, throughput is 1.2 ~ 2 L/min, cultivates 24 h, obtain under the conditions of 29 DEG C
To the fermentation culture containing L-arabinose；
5) by step 4) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 ~ 120 min, is cooled to 45 DEG C, with step
Rapid 2) resulting solution merges, and centrifugal filtration obtains one-level filtrate；Centrifugation gained precipitation plus the water centrifugal filtration again of 2 times amount, obtain
To two grades of filtrates and precipitation；Gained two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three-level filtrate；Merge three times from
Heart gained filtrate, reduced pressure concentration, prepared disintoxicating product.
Step 1) group of described bacterium culture medium is divided into (mass percent)：Dusty yeast 1 %, glucose 2 %, peptone 2
%, pH are natural, and sterilize under the conditions of 121 DEG C 20 min.
Step 4) group of described fermentation medium is divided into (mass percent)：Yeast extract 0.2 %, peptone 0.3 %,
KH2PO40.1 %, (NH4)2SO40.5 %, MgSO4·7H2O 0.05 %, sterilize under the conditions of 121 DEG C 20 min.
By step 5）Prepared disintoxicating product separates through centrifuge, and the solid phase obtaining is spray-dried, and makes powder, then
Add auxiliary material, make bromatology, the acceptable any type of product of health food.
Preferably, by step 5）Prepared disintoxicating product separates through centrifuge, and the solid phase obtaining is spray-dried, and makes
Powder, adds auxiliary material, makes piece for oral administration, chewable tablet, buccal tablets, granule, capsule, tea in bag.
Preferably, by step 5）Prepared disintoxicating product adds flavouring and drinking water, makes beverage class product.
It is an advantage of the invention that：
1) present invention is obtained for fermentable, the no dangerous material such as chemical synthesis material in preparation process, wine brewing ferment used
Female simplicity is easy to get；
2) after saccharomyces cerevisiae bacterial classification Amplification Culture, alcohol dehydrogenase is obtained by cell breaking technology and acetaldehyde dehydrogenase makes introducing
Ethanol first decompose rapidly in stomach, accelerate the metabolic process to alcohol for the alcohol user, effectively improve the metabolic capability to alcohol,
Alleviate alcohol in human body due to savings time length injury that human body is caused, reduce ethanol and enter blood circulation system and enter liver
Dirty, to mitigate burden of liver.
3) after fermentation of maize peel enzymolysis is obtained L-arabinose, improves the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase, makes
Ethanol and acetaldehyde faster metabolism, protect stomach lining, reduce alcohol to the damage of stomach lining so that alcohol amount retained phase in human body
To less；
4), because the presence mellow in taste of L-arabinose, sugariness is moderate, is also simultaneously suitable for all for the disintoxicating product being obtained
Prohibit sucrose crowd as diabetes, cardiovascular disease etc. to use；
5) this product make people occur dizzy, blush, the heartbeat even unclear phenomenon of expression of overrunning reduces, and has significantly anti-
Liquor-saturated antialcoholism action.Its effect sobered up and Time of Administration before drinking, when drinking, drink after have effect, before drinking and drink
When take the effect that this product has pre- preventing drunkenness well, drunk after take the work that this product can effectively act as sobering up
With.
6) in preparing, maize peel used is the Main By product of starch industry, and wide material sources are cheap, and the nutrition of culture medium becomes
It is evenly distributed, be conducive to being fully contacted and absorbing of mushroom trophosome；
7) in incubation, physico chemical factor is easily controllable, production technology specification, and with short production cycle, product quality is stable, produces
Amount is high, low to Preparation equipment and site requirements, is easy to industrialized production, and not season controlled.
Specific embodiment
The specific embodiment of form by the following examples, is described in further detail to the above of the present invention.But
This scope being interpreted as the above-mentioned theme of the present invention should not be only limitted to following examples.All realized based on the above of the present invention
Technology belong to the scope of the present invention.
The group of bacterium culture medium is divided into (mass percent)：Dusty yeast 1 %, glucose 2 %, peptone 2 %, pH are natural,
Sterilize under the conditions of 121 DEG C 20 min.
The group of fermentation medium is divided into (mass percent)：Yeast extract 0.2 %, peptone 0.3 %, KH2PO40.1 %,
(NH4)2SO40.5 %, MgSO4·7H2O 0.05 %, sterilize under the conditions of 121 DEG C 20 min.
Embodiment 1
1) Spawn incubation：Saccharomyces cerevisiae bacterial classification is inoculated in equipped with the fermentation tank of bacterium culture medium, liquid amount 10 %, inoculation
Measure 7 %, air quantity is 1：0.6, pH 7, rotating speed 200 rpm/min, 28 DEG C of cultivation temperature, cultivate 16 h, obtain bacteria culture fluid；
2) by step 1) centrifugation of the bacteria culture fluid prepared, abandoning supernatant is precipitated, with 0.066 mol/L phosphoric acid hydrogen two
Sodium buffer solution 2 times, retains somatic cells, adds the Tris-cl containing 5 % 50 mM（pH 8.0）, 0.2 % 1 mM
EDTA, the buffer solution of the NaCl of 2 % 100 mM, carry out clasmatosis with Constant high-pressure cell crusher, are contained
Alcohol dehydrogenase and the solution of acetaldehyde dehydrogenase；
3) maize peel enzymolysis liquid preparation：Maize peel crosses 60 mesh sieves after crushed, according to 1：8 part by weight adds purified water, adjusts
To 6.0, agitation revolution is 120 rpm/min to solution pH value, and 6.0 % according to maize peel dry add hemicellulose degradation to be combined
Enzyme, controlled enzymatic hydrolysis temperature is 80 DEG C, enzymolysis time 30 h, then filters through plate and frame filter press, removes waste residue, the enzymolysis obtaining
In liquid, the weight percent concentration of sugar is 30 %；
4) fermented and cultured：By step 3) to be concentrated into weight percent concentration be 30% for the maize peel enzymolysis liquid prepared, the bottom of as
Thing, adds equipped with the fermentation tank of fermentation medium, and accesses steps 1 by 5 % inoculum concentrations) bacteria culture fluid prepared, ferments
Canned liquid measure 60 %, mixing speed 400 r/min, throughput is 2 L/min, cultivates 24 h under the conditions of 29 DEG C, obtain containing L- Ah
Draw the fermentation culture of uncle's sugar；
5) by step 4) fermentation culture that obtains adds water stirring, 100 DEG C of heating 60 min, is cooled to 45 DEG C, with step 2)
Resulting solution merges, and rotating speed 6000 rpm/min centrifugal filtration 5min obtains one-level filtrate；Centrifugation gained precipitation adds 2 times amount
Water centrifugal filtration again, obtains two grades of filtrates and precipitation；Gained two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three
Level filtrate；Merge three centrifugation gained filtrates, reduced pressure concentration, disintoxicating product is obtained；
By step 5）Prepared disintoxicating product separates through centrifuge, isolates solid phase under the conditions of 50 DEG C about, 6000 r/min
And liquid phase, solid phase is utilized drying machine with centrifugal spray, EAT be 160 DEG C, pressure be 0.5 MPa under conditions of, carry out
Spray drying makes powder, according to the preparation of following prescription：
Powder 50 g of the present invention
Starch 20 g
Dextrin 30 g
50% appropriate amount of ethanol
Magnesium stearate 1g
Make 1000
Preparation method：Weigh powder of the present invention, starch, dextrin mix, and 50% ethanol is once added mixed powder by Sq
In end, during addition, dispersion face is big, mixes, makes softwood, make wet grain by 18-20 mesh nylon mesh, does for less than 60 DEG C
Dry, less than 70 DEG C can be risen to when closely dry, accelerate drying, dry granular moisture controls in 1.5 below %.Whole with mesh sieve during wet grain to make
Grain, shines fine powder in dry granular, mixes with the magnesium stearate sieving, and then mixes with dry particl again, after measuring content, calculates piece
Weight, punch die compressing tablet.
Embodiment 2
1) Spawn incubation：Saccharomyces cerevisiae bacterial classification is inoculated in equipped with the fermentation tank of bacterium culture medium, liquid amount 10 %, inoculation
Measure 7 %, air quantity is 1：0.6, pH 7, rotating speed 200 rpm/min, 30 DEG C of cultivation temperature, cultivate 16 h, obtain bacteria culture fluid；
2) by step 1) centrifugation of the bacteria culture fluid prepared, abandoning supernatant is precipitated, with 0.066 mol/L phosphoric acid hydrogen two
Sodium buffer solution 3 times, retains somatic cells, adds the Tris-cl containing 5 % 50 mM（pH 8.0）, 0.2 % 1 mM
EDTA, the buffer solution of the NaCl of 2 % 100 mM, carry out clasmatosis with Constant high-pressure cell crusher, are contained
Alcohol dehydrogenase and the solution of acetaldehyde dehydrogenase；
3) maize peel enzymolysis liquid preparation：Maize peel crosses 60 mesh sieves after crushed, according to 1：3 part by weight adds purified water, adjusts
To 5.0, agitation revolution is 50 rpm/min to solution pH value, and 0.3 % according to maize peel dry adds hemicellulose degradation complex enzyme,
Controlled enzymatic hydrolysis temperature is 45 DEG C, enzymolysis time 8 h, then filters through plate and frame filter press, removes waste residue, in the enzymolysis liquid obtaining
The weight percent concentration of sugar is 10 %；
4) fermented and cultured：By step 3) to be concentrated into weight percent concentration be 20 % for the maize peel enzymolysis liquid prepared, the bottom of as
Thing, adds equipped with the fermentation tank of fermentation medium, and accesses steps 1 by 5 % inoculum concentrations) bacteria culture fluid prepared, ferments
Canned liquid measure 60 %, mixing speed 400 r/min, throughput is 1.2 L/min, cultivates 24 h, obtain containing L- under the conditions of 29 DEG C
The fermentation culture of arabinose；
5) by step 4) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 min, is cooled to 45 DEG C, with step 2)
Resulting solution merges, and rotating speed 6000 rpm/min centrifugal filtration 5min obtains one-level filtrate；Centrifugation gained precipitation adds 2 times amount
Water centrifugal filtration again, obtains two grades of filtrates and precipitation；Gained two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three
Level filtrate；Merge three centrifugation gained filtrates, reduced pressure concentration, prepared disintoxicating product；
By step 5）Prepared disintoxicating product separates through centrifuge, isolates solid phase under the conditions of 50 DEG C about, 6000 r/min
And liquid phase, solid phase is utilized drying machine with centrifugal spray, EAT be 160 DEG C, pressure be 0.5 MPa under conditions of, carry out
Spray drying makes powder, according to the preparation of following prescription：
Powder 1 ~ 2% of the present invention
Citric acid 8 ~ 10 %
Sodium acid carbonate 6 ~ 10 %
Icing Sugar 70 ~ 90 %
Appropriate lemon yellow
Appropriate sweetener
Preparation method：(1) citric acid is worn into fine powder, be dried, take powder of the present invention to mix with citric acid, add lemon yellow
Diluted Alcohol solution, mixes, and pelletizes, and is dried to acid material；(2) take Icing Sugar, sodium acid carbonate to mix respectively, add lemon
Huang, saccharin sodium water solution and flavoring essence, mix, and pelletize, are dried to alkaline dispensing, and the acid being dried, alkali material are mixed by (3)
Close；(4) dispense, make granule.
Embodiment 3
1) Spawn incubation：Saccharomyces cerevisiae bacterial classification is inoculated in equipped with the fermentation tank of bacterium culture medium, liquid amount 10 %, inoculation
Measure 7 %, air quantity is 1：0.6, pH 7, rotating speed 200 rpm/min, 29 DEG C of cultivation temperature, cultivate 16 h, obtain bacteria culture fluid；
2) by step 1) centrifugation of the bacteria culture fluid prepared, abandoning supernatant is precipitated, with 0.066 mol/L phosphoric acid hydrogen two
Sodium buffer solution 2 times, retains somatic cells, adds the Tris-cl containing 5 % 50 mM（pH 8.0）, 0.2 % 1 mM
EDTA, the buffer solution of the NaCl of 2 % 100 mM, carry out clasmatosis with constant high-pressure cell crusher, are contained
Alcohol dehydrogenase and the solution of acetaldehyde dehydrogenase；
3) maize peel enzymolysis liquid preparation：Maize peel crosses 60 mesh sieves after crushed, according to 1：12 part by weight adds purified water,
Reconcile pH value to 5.5, agitation revolution is 80 rpm/min, 3.0 % according to maize peel dry add hemicellulose degradation to be combined
Enzyme, controlled enzymatic hydrolysis temperature is 60 DEG C, enzymolysis time 40 h, then filters through plate and frame filter press, removes waste residue, the enzymolysis obtaining
In liquid, the weight percent concentration of sugar is 20 %；
4) fermented and cultured：By step 3) to be concentrated into weight percent concentration be 40 % for the maize peel enzymolysis liquid prepared, the bottom of as
Thing, adds equipped with the fermentation tank of fermentation medium, and accesses steps 1 by 5 % inoculum concentrations) bacteria culture fluid prepared, ferments
Canned liquid measure 60 %, mixing speed 400 r/min, throughput is 1 L/min, cultivates 24 h under the conditions of 29 DEG C, obtain containing L- Ah
Draw the fermentation culture of uncle's sugar；
5) by step 4) fermentation culture that obtains adds water stirring, 100 DEG C of heating 120 min, is cooled to 45 DEG C, with step
2) resulting solution merges, and rotating speed 6000 rpm/min centrifugal filtration 5min obtains one-level filtrate；Centrifugation gained precipitation plus 2 times amount
Water centrifugal filtration again, obtain two grades of filtrates and precipitation；Gained two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains
Three-level filtrate；Merge three centrifugation gained filtrates, reduced pressure concentration, prepared disintoxicating product；
By step 5）Prepared disintoxicating product is prepared according to following prescription：
Disintoxicating product 10% of the present invention
White granulated sugar 6%
Protein sugar 0.03 %
Honey element 0.06 %
Citric acid 0.20 %
Malic acid 0.05 %
Sodium citrate 0.02 %
Salt 0.03 %
C.M.C（FH9） 0.06 %
Xanthans 0.06 %
Sodium Benzoate 0.02 %
Preparation method：According to the above ratio disintoxicating product of the present invention and auxiliary material are dissolved in pure water, are quantitatively adding in material-compound tank and stir
Mix uniformly, filter, carry out through sterilization machine being cooled to after flash-sterilization 98 DEG C filling；Sterilized through 2 minutes after sealing, spray
It is cooled to less than 40 DEG C, be incubated 7 days at 30 DEG C about, inspection, vanning warehouse-in.
The present invention can also add any composition with effects of dispelling effects of alcohol and protecting liver and/auxiliary material, makes bromatology, health food
Learn acceptable any type of product.
Below by mouse experiment, the present invention is described further, and in below testing, selected mouse is purchased from China
Academy of Medical Sciences institute of lab animals.
Take mouse 70（NIH）, male and female half and half, it is divided into 7 groups at random, I group is to gavage physiological saline group merely, gives for II group
Physiological saline tests previous evening fasting for solids and liquids with wine group, III group to the VII group present invention to Different adding amount with wine group.Start I
Group and II group of gavage physiological saline 0.3 ml/10 g, III group to VII group mouse with Isodose containing Different adding amount this
The physiological saline of bright disintoxicating product（10 mg/mL、100 mg/mL、300 mg/mL、600 mg/mL、900 mg/mL）Gavage, 30
I group of still gavage physiological saline 0.2 ml/10 g, II group to VII group equal gavage white wine after min（54°）0.2 ml/10 g, then through 30
After min, each group mouse orbit is taken with blood, and measures the concentration of alcohol in blood with gas chromatography.
The mensure of ethanol in blood concentration：
1. sample preparation
（1）Prepare to draw whole blood 0.2 ml to be measured, internal standard solution（Containing normal propyl alcohol 0.8 mg/ml）0.3 ml in 10 ml empty bottles,
Seal.
（2）The making of ethanol calibration curve.Blank blood 0.2 ml, internal standard solution（Containing normal propyl alcohol 0.8 mg/ml）0.3 ml in
10 ml
In empty bottle, add 0.2,0.4,1.0,2.0,3.0,4.0,5.0,7.0,8.0,9.0,10.0 μ l respectively, seal.Second
Alcohol peak area compares ethanol addition with normal propyl alcohol peak area（μl）Make calibration curve.Result is one and passes through the straight of initial point
Line, existing relation is good, and its linear equation is Y=2.5X；R=0.993
2. instrument and analysis condition
GC conditions：Enter sample temperature：200 ℃；Detector temperature：300 ℃；Furnace temperature：80 ℃；Chromatographic column：HP-
FFAP 25×0.32×0.17μm ；Stigma pressure 8 Psi；Shunting outlet：30 ml/min；Tail blows：20 ml/min；Air：44
Psi；H2：30 Psi；Input mode：Split sampling；Head space instrument condition：Oily melting temperatur：80 ℃；Pipe/valve temperature：85℃；Sample
Equilibration time：10 min；N2 flow：20 ml/min；Tail blows：15 ml/min；Input mode：Pressurize 10 S；Prevention and control：2 S；Enter
Sample 60 s.
Table 1：The test experience of ethanol in mouse blood
| Group | I group | II group | III group | IV group | V group | VI group | VII group |
| Ethanol in blood concentration（mg/ml） | 0 | 10.81±1.05 | 9.31±2.21 | 8.55±1.32 | 6.68±2.02 | 5.41±1.98 | 4.56±1.61 |
As can be seen from Table 1, II group of significant difference with mouse ethanol in blood concentration in other groups（P ＜ 0.05）, this
Bright disintoxicating product can significantly reduce concentration of alcohol in mouse blood, illustrates that disintoxicating product of the present invention has antialcoholism function.
Disintoxicating product of the present invention can use before drinking or during drinking, described disintoxicating product can with appoint
What functional components such as has that hepatoprotective effect, liver protection effect, strengthen immunity effect etc. are compound to become alcohol-neutralize healthy product.
Obviously, above-described embodiment is only intended to clearly illustrate example, rather than the restriction to embodiment.For
Change or the change of other multi-forms for those of ordinary skill in the art, can also be made on the basis of the above description
Dynamic.And the obvious change thus amplified out or variation are still in the protection domain of the invention.
Claims (6)
1. a kind of preparation method of disintoxicating product is it is characterised in that comprise the following steps：
1) Spawn incubation：Saccharomyces cerevisiae bacterial classification is inoculated in equipped with the fermentation tank of bacterium culture medium, liquid amount 10 %, inoculation
Measure 7 %, air quantity is 1：0.6, pH 7, rotating speed 200 rpm/min, 28 ~ 30 DEG C of cultivation temperature, cultivate 16 h, obtain bacterial classification training
Nutrient solution；
2) by step 1) centrifugation of the bacteria culture fluid prepared, abandoning supernatant is precipitated, with 0.066 mol/L phosphoric acid hydrogen two
Sodium buffer solution 2 ~ 3 times, retains somatic cells, adds the Tris-cl containing 5 % 50 mM（pH 8.0）、0.2 % 1 mM
EDTA, the buffer solution of the NaCl of 2 % 100 mM, carry out clasmatosis with high pressure crusher, obtain containing alcohol dehydrogenase
Solution with acetaldehyde dehydrogenase；
3) maize peel enzymolysis liquid preparation：Maize peel crosses 60 mesh sieves after crushed, according to 1：3~1：12 part by weight adds purifying
Water, conciliation pH value to 5.0 ~ 6.0, agitation revolution is 50 ~ 120 rpm/min, and 0.3 ~ 6.0 % according to maize peel dry adds half
Cellulose degradation complex enzyme, controlled enzymatic hydrolysis temperature is 45 ~ 80 DEG C, enzymolysis time 8 ~ 40 h, then filters through plate and frame filter press,
Remove waste residue, in the enzymolysis liquid obtaining, the weight percent concentration of sugar is 10 ~ 30 %；
4) fermented and cultured：By step 3) to be concentrated into weight percent concentration be 20 ~ 40 % for the maize peel enzymolysis liquid prepared, with this
For substrate, add equipped with the fermentation tank of fermentation medium, and by 5 % inoculum concentrations access steps 1) bacteria culture fluid prepared,
Fermentation tank liquid amount 60 %, mixing speed 400 r/min, throughput is 1.2 ~ 2 L/min, cultivates 24 h, obtain under the conditions of 29 DEG C
To the fermentation culture containing L-arabinose；
5) by step 4) fermentation culture that obtains adds water stirring, 100 DEG C of heating 30 ~ 120 min, is cooled to 45 DEG C, with step
Rapid 2) resulting solution merges, and centrifugal filtration obtains one-level filtrate；Centrifugation gained precipitation plus the water centrifugal filtration again of 2 times amount, obtain
To two grades of filtrates and precipitation；Gained two-stage precipitation adds the water centrifugal filtration of 2 times amount again, obtains three-level filtrate；Merge three times from
Heart gained filtrate, reduced pressure concentration, prepared disintoxicating product.
2. a kind of preparation method of disintoxicating product according to claim 1 is it is characterised in that step 1) described Spawn incubation
The group of base is divided into (mass percent)：Dusty yeast 1 %, glucose 2 %, peptone 2 %, pH are natural, sterilize under the conditions of 121 DEG C
20 min.
3. a kind of preparation method of disintoxicating product according to claim 1 is it is characterised in that step 4) described fermented and cultured
The group of base is divided into (mass percent)：Yeast extract 0.2 %, peptone 0.3 %, KH2PO40.1 %, (NH4)2SO40.5 %,
MgSO4·7H2O 0.05 %, sterilize under the conditions of 121 DEG C 20 min.
4. a kind of preparation method of disintoxicating product according to claim 1 is it is characterised in that by step 5）Prepared relieves the effect of alcohol
Product separates through centrifuge, and the solid phase obtaining is spray-dried, and makes powder, adds auxiliary material, makes bromatology, health care food
The acceptable any type of product of conduct and learning.
5. a kind of preparation method of disintoxicating product according to claim 1 is it is characterised in that by step 5）Prepared relieves the effect of alcohol
Product separates through centrifuge, and the solid phase obtaining is spray-dried, and makes powder, adds auxiliary material, makes piece for oral administration, chewing
Piece, buccal tablets, granule, capsule, tea in bag, pressed candy, soft sweets, hard candy.
6. a kind of preparation method of disintoxicating product according to claim 1 is it is characterised in that by step 5）Prepared relieves the effect of alcohol
Product adds flavouring and drinking water, makes beverage class product.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853532A (en) * | 2017-12-14 | 2018-03-30 | 江西颐迪科技有限公司 | A kind of Antialcoholic liver-protecting protect liver solid beverage and preparation method thereof |
| CN109329941A (en) * | 2018-12-19 | 2019-02-15 | 九仙尊霍山石斛股份有限公司 | A kind of Dendrobidium huoshanness relieves the effect of alcohol particle and preparation method thereof |
| CN114032256A (en) * | 2021-10-15 | 2022-02-11 | 山东寿光巨能金玉米开发有限公司 | Method for co-producing ethanol and L-arabinose by using corn bran |
| US20240216479A1 (en) * | 2021-05-05 | 2024-07-04 | Bing Biotech Limited | Encapsulation of dual-enzyme composition for preventing, treating and/or alleviating veisalgia and symptoms associated therewith |
Citations (1)
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| CN105815788A (en) * | 2016-03-31 | 2016-08-03 | 天津市中英保健食品有限公司 | Healthy substitute sugar and method for preparing healthy substitute sugar through liquid state fermentation technology |
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| CN105815788A (en) * | 2016-03-31 | 2016-08-03 | 天津市中英保健食品有限公司 | Healthy substitute sugar and method for preparing healthy substitute sugar through liquid state fermentation technology |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853532A (en) * | 2017-12-14 | 2018-03-30 | 江西颐迪科技有限公司 | A kind of Antialcoholic liver-protecting protect liver solid beverage and preparation method thereof |
| CN109329941A (en) * | 2018-12-19 | 2019-02-15 | 九仙尊霍山石斛股份有限公司 | A kind of Dendrobidium huoshanness relieves the effect of alcohol particle and preparation method thereof |
| US20240216479A1 (en) * | 2021-05-05 | 2024-07-04 | Bing Biotech Limited | Encapsulation of dual-enzyme composition for preventing, treating and/or alleviating veisalgia and symptoms associated therewith |
| CN114032256A (en) * | 2021-10-15 | 2022-02-11 | 山东寿光巨能金玉米开发有限公司 | Method for co-producing ethanol and L-arabinose by using corn bran |
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Application publication date: 20170215 |