CN114467027B - 用于分子检测的光触发核酸构建体和方法 - Google Patents

用于分子检测的光触发核酸构建体和方法

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Publication number
CN114467027B
CN114467027B CN202080052424.6A CN202080052424A CN114467027B CN 114467027 B CN114467027 B CN 114467027B CN 202080052424 A CN202080052424 A CN 202080052424A CN 114467027 B CN114467027 B CN 114467027B
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China
Prior art keywords
nucleic acid
photo
primer pair
primer
light
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CN202080052424.6A
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English (en)
Chinese (zh)
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CN114467027A (zh
Inventor
奥尔延·哈斯比
罗伯特·G·库梅里斯
裴雷
柯尔斯顿·A·约翰逊
杰西卡·C·艾伯特
阿伦·马尼卡姆
特朗·T·范
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Insilixa Inc
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Insilixa Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080052424.6A 2019-05-20 2020-05-20 用于分子检测的光触发核酸构建体和方法 Active CN114467027B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962850239P 2019-05-20 2019-05-20
US62/850,239 2019-05-20
PCT/US2020/033884 WO2020236992A1 (en) 2019-05-20 2020-05-20 Photo-triggered nucleic acid constructs and methods for molecular detection

Publications (2)

Publication Number Publication Date
CN114467027A CN114467027A (zh) 2022-05-10
CN114467027B true CN114467027B (zh) 2025-07-15

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Country Status (5)

Country Link
US (1) US20220282320A1 (enExample)
EP (1) EP3973289A4 (enExample)
JP (1) JP7611859B2 (enExample)
CN (1) CN114467027B (enExample)
WO (1) WO2020236992A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7724632B2 (ja) * 2020-04-23 2025-08-18 東ソー株式会社 標的核酸の光制御増幅試薬、光制御増幅方法および検出方法
WO2022147134A1 (en) * 2020-12-30 2022-07-07 10X Genomics, Inc. Molecular arrays and methods for generating and using the arrays
WO2022147139A1 (en) * 2020-12-30 2022-07-07 10X Genomics, Inc. Methods and compositions for light-controlled surface patterning using a polymer
WO2024196728A2 (en) * 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Nucleic acid probes for combined sequencing and spatial analysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015128272A2 (en) * 2014-02-26 2015-09-03 Ventana Medical Systems, Inc. Photo-selective method for biological sample analysis field

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
AU2003213652A1 (en) * 2002-03-01 2003-09-16 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US9045522B2 (en) * 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US9371348B2 (en) * 2006-11-27 2016-06-21 The Trustees Of The University Of Pennsylvania Photocleavable oligonucleotide and uses thereof
KR20130022437A (ko) * 2011-08-22 2013-03-07 삼성전자주식회사 광분해성 화합물을 사용한, 비특이적 증폭의 감소를 위한 신규한 ｐｃｒ 방법
EP2932270B1 (en) * 2012-12-11 2018-10-24 GE Healthcare Bio-Sciences Corp. Photoactivated chemical bleaching of dyes
US9745618B2 (en) * 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
WO2017214417A1 (en) * 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015128272A2 (en) * 2014-02-26 2015-09-03 Ventana Medical Systems, Inc. Photo-selective method for biological sample analysis field

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Light-triggered polymerase chain reaction;YOUNG DOUGLAS D. 等;CHEMICAL COMMUNICATIONS;20071114;第462-464页 *

Also Published As

Publication number Publication date
EP3973289A4 (en) 2023-05-03
EP3973289A1 (en) 2022-03-30
WO2020236992A1 (en) 2020-11-26
CN114467027A (zh) 2022-05-10
US20220282320A1 (en) 2022-09-08
JP2022533228A (ja) 2022-07-21
JP7611859B2 (ja) 2025-01-10

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