JP7611859B2 - 光によりトリガーされる核酸構築物および分子検出のための方法 - Google Patents

光によりトリガーされる核酸構築物および分子検出のための方法 Download PDF

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JP7611859B2
JP7611859B2 JP2021569060A JP2021569060A JP7611859B2 JP 7611859 B2 JP7611859 B2 JP 7611859B2 JP 2021569060 A JP2021569060 A JP 2021569060A JP 2021569060 A JP2021569060 A JP 2021569060A JP 7611859 B2 JP7611859 B2 JP 7611859B2
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nucleic acid
light
primer pair
primer
construct
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JP2022533228A (ja
JPWO2020236992A5 (enExample
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アルジャン ハッシビ，
ロバート ジー． クイメリス，
レイ ペイ，
カーステン エー． ジョンソン，
ジェシカ シー． イーバート，
アルン マニカム，
トラン ティー． ヴァン，
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インシリクサ， インコーポレイテッド
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021569060A 2019-05-20 2020-05-20 光によりトリガーされる核酸構築物および分子検出のための方法 Active JP7611859B2 (ja)

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US201962850239P 2019-05-20 2019-05-20
US62/850,239 2019-05-20
PCT/US2020/033884 WO2020236992A1 (en) 2019-05-20 2020-05-20 Photo-triggered nucleic acid constructs and methods for molecular detection

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JP2022533228A JP2022533228A (ja) 2022-07-21
JPWO2020236992A5 JPWO2020236992A5 (enExample) 2023-05-29
JP7611859B2 true JP7611859B2 (ja) 2025-01-10

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US (1) US20220282320A1 (enExample)
EP (1) EP3973289A4 (enExample)
JP (1) JP7611859B2 (enExample)
CN (1) CN114467027B (enExample)
WO (1) WO2020236992A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7724632B2 (ja) * 2020-04-23 2025-08-18 東ソー株式会社 標的核酸の光制御増幅試薬、光制御増幅方法および検出方法
EP4271509A1 (en) * 2020-12-30 2023-11-08 10X Genomics, Inc. Molecular arrays and methods for generating and using the arrays
US20220314187A1 (en) * 2020-12-30 2022-10-06 10X Genomics, Inc. Methods and compositions for light-controlled surface patterning using a polymer
WO2024196728A2 (en) * 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Nucleic acid probes for combined sequencing and spatial analysis

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2013005803A (ja) 2000-05-19 2013-01-10 Luminex Corp 核酸の検出用物質及び検出方法
US20130052690A1 (en) 2011-08-22 2013-02-28 Samsung Electronics Co., Ltd. Pcr primer and method for reducing non-specific nucleic acid amplification using a photolabile protecting group
JP2017507656A (ja) 2014-02-26 2017-03-23 ヴェンタナ メディカル システムズ， インク． 生物学的試料分析のための光選択的方法

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Publication number Priority date Publication date Assignee Title
EP1481088B1 (en) * 2002-03-01 2008-09-17 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US9045522B2 (en) * 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US9371348B2 (en) * 2006-11-27 2016-06-21 The Trustees Of The University Of Pennsylvania Photocleavable oligonucleotide and uses thereof
EP2932270B1 (en) * 2012-12-11 2018-10-24 GE Healthcare Bio-Sciences Corp. Photoactivated chemical bleaching of dyes
US9745618B2 (en) * 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
WO2017214417A1 (en) * 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013005803A (ja) 2000-05-19 2013-01-10 Luminex Corp 核酸の検出用物質及び検出方法
US20130052690A1 (en) 2011-08-22 2013-02-28 Samsung Electronics Co., Ltd. Pcr primer and method for reducing non-specific nucleic acid amplification using a photolabile protecting group
JP2017507656A (ja) 2014-02-26 2017-03-23 ヴェンタナ メディカル システムズ， インク． 生物学的試料分析のための光選択的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Chem Commun(Camb)(2008)，Vol.28,No.4，p.462-464
J. AM. CHEM. SOC.(2010)，Vol.132，p.6183-6193

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EP3973289A1 (en) 2022-03-30
JP2022533228A (ja) 2022-07-21
US20220282320A1 (en) 2022-09-08
EP3973289A4 (en) 2023-05-03
CN114467027A (zh) 2022-05-10
WO2020236992A1 (en) 2020-11-26
CN114467027B (zh) 2025-07-15

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