CN114450403A - 用于在枫糖尿症(msud)的治疗中使用的酶的生物合成 - Google Patents

用于在枫糖尿症(msud)的治疗中使用的酶的生物合成 Download PDF

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CN114450403A
CN114450403A CN202080058809.3A CN202080058809A CN114450403A CN 114450403 A CN114450403 A CN 114450403A CN 202080058809 A CN202080058809 A CN 202080058809A CN 114450403 A CN114450403 A CN 114450403A
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residue
seq
residue corresponding
host cell
enzyme
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Chinese (zh)
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R·杰恩
R·普特曼
K·齐默曼
P·鲍尔
D·A·卡林
L·斯通
A·C·塔克
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Synchronic Operation Co
Ginkgo Bioworks Inc
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Synchronic Operation Co
Ginkgo Bioworks Inc
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CN202080058809.3A 2019-06-21 2020-06-19 用于在枫糖尿症(msud)的治疗中使用的酶的生物合成 Pending CN114450403A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962865129P 2019-06-21 2019-06-21
US201962864875P 2019-06-21 2019-06-21
US62/864,875 2019-06-21
US62/865,129 2019-06-21
PCT/US2020/038813 WO2020257707A1 (en) 2019-06-21 2020-06-19 Biosynthesis of enzymes for use in treatment of maple syrup urine disease (msud)

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CN114450403A true CN114450403A (zh) 2022-05-06

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US (2) US20220348933A1 (enExample)
EP (2) EP3986432A4 (enExample)
JP (1) JP2022537214A (enExample)
KR (1) KR20220042350A (enExample)
CN (1) CN114450403A (enExample)
AU (1) AU2020297586A1 (enExample)
CA (1) CA3144416A1 (enExample)
IL (1) IL289123A (enExample)
WO (2) WO2020257610A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US20220031771A1 (en) * 2020-07-31 2022-02-03 Iowa State University Research Foundation, Inc. Microencapsulated and chromosome integrated compositions for l-dopa microbiome therapy
CN113484435B (zh) * 2021-07-05 2023-04-07 中国人民解放军空军军医大学 检测血浆支链氨基酸和支链a酮酸水平的物质的应用及产品
WO2024064918A2 (en) * 2022-09-23 2024-03-28 Novome Biotechnologies, Inc. Recombinant fusion polypeptides for secreting soluble, heterologous cargo
WO2025046062A1 (en) 2023-08-31 2025-03-06 Snipr Biome Aps A novel type of crispr/cas system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017123676A1 (en) * 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
US20170232043A1 (en) * 2015-06-10 2017-08-17 Synlogic, Inc. Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid
CN108559735A (zh) * 2018-05-10 2018-09-21 江南大学 一种亮氨酸脱氢酶突变体的构建及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6119738B2 (ja) * 2012-03-30 2017-04-26 味の素株式会社 改変ロイシン脱水素酵素
US10233467B2 (en) * 2012-08-17 2019-03-19 Easel Biotechnologies, Llc Two-stage production of higher alcohols
US10059969B1 (en) * 2014-10-03 2018-08-28 Abbvie Inc. Process for the preparation of (S)-2-amino-non-8-enoic acid
US10610546B2 (en) * 2015-05-13 2020-04-07 Synlogic Operating Company Inc. Bacteria engineered to reduce hyperphenylalaninemia
CN104774813B (zh) * 2015-04-20 2019-02-26 南京工业大学 一种亮氨酸脱氢酶及其制备方法和应用
CN108103038B (zh) * 2017-12-15 2021-03-02 江南大学 一种合成l-苯甘氨酸的单细胞工厂及其构建与应用
CN109321538A (zh) * 2018-09-07 2019-02-12 江南大学 一种基于数据库基因挖掘方法获得的亮氨酸脱氢酶
CN109679978B (zh) * 2018-12-12 2020-12-29 浙江工业大学 一种用于制备l-2-氨基丁酸的重组共表达体系及其应用
WO2021146394A1 (en) * 2020-01-14 2021-07-22 Synlogic Operating Company, Inc. Optimized bacteria engineered to treat disorders involving the catabolism of leucine, isoleucine, and/or valine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170232043A1 (en) * 2015-06-10 2017-08-17 Synlogic, Inc. Bacteria engineered to treat disorders involving the catabolism of a branched chain amino acid
WO2017123676A1 (en) * 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
CN108559735A (zh) * 2018-05-10 2018-09-21 江南大学 一种亮氨酸脱氢酶突变体的构建及其应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GENBANK: "leucine dehydrogenase，GenBank：WP_078694365.1", GENBANK, 10 March 2017 (2017-03-10) *

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