CN114436711A - Microbial organic fertilizer for preventing and treating cotton wilt and preparation method thereof - Google Patents
Microbial organic fertilizer for preventing and treating cotton wilt and preparation method thereof Download PDFInfo
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- CN114436711A CN114436711A CN202210041121.5A CN202210041121A CN114436711A CN 114436711 A CN114436711 A CN 114436711A CN 202210041121 A CN202210041121 A CN 202210041121A CN 114436711 A CN114436711 A CN 114436711A
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 76
- 230000000813 microbial effect Effects 0.000 title claims abstract description 56
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 110
- 230000004151 fermentation Effects 0.000 claims abstract description 110
- 239000000843 powder Substances 0.000 claims abstract description 103
- 210000003608 fece Anatomy 0.000 claims abstract description 49
- 239000010871 livestock manure Substances 0.000 claims abstract description 49
- 241000287828 Gallus gallus Species 0.000 claims abstract description 46
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000003337 fertilizer Substances 0.000 claims abstract description 33
- 239000004575 stone Substances 0.000 claims abstract description 30
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 22
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000006040 Prunus persica var persica Nutrition 0.000 claims abstract description 22
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000661 sodium alginate Substances 0.000 claims abstract description 22
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 22
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 22
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 21
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 21
- 210000003278 egg shell Anatomy 0.000 claims abstract description 21
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000004327 boric acid Substances 0.000 claims abstract description 20
- DSSYKIVIOFKYAU-XVKPBYJWSA-N (1s,4r)-4,7,7-trimethylbicyclo[2.2.1]heptan-3-one Chemical compound C1C[C@@]2(C)C(=O)C[C@H]1C2(C)C DSSYKIVIOFKYAU-XVKPBYJWSA-N 0.000 claims abstract description 18
- 229920002752 Konjac Polymers 0.000 claims abstract description 17
- 235000010485 konjac Nutrition 0.000 claims abstract description 17
- 235000013312 flour Nutrition 0.000 claims abstract description 16
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 15
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 15
- 244000247812 Amorphophallus rivieri Species 0.000 claims abstract description 14
- 235000001206 Amorphophallus rivieri Nutrition 0.000 claims abstract description 14
- 239000000252 konjac Substances 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 13
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 66
- 239000000243 solution Substances 0.000 claims description 59
- 239000011248 coating agent Substances 0.000 claims description 57
- 238000000576 coating method Methods 0.000 claims description 57
- 238000003756 stirring Methods 0.000 claims description 30
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 28
- 241000193747 Bacillus firmus Species 0.000 claims description 28
- 241000194103 Bacillus pumilus Species 0.000 claims description 28
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 28
- 229940005348 bacillus firmus Drugs 0.000 claims description 28
- 244000144730 Amygdalus persica Species 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 239000011247 coating layer Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000007771 core particle Substances 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 241001671204 Stemona Species 0.000 claims description 10
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 claims description 10
- 201000010099 disease Diseases 0.000 claims description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000001125 extrusion Methods 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000003595 mist Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 20
- 230000002265 prevention Effects 0.000 abstract description 9
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 240000006413 Prunus persica var. persica Species 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 description 59
- 230000000052 comparative effect Effects 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000012792 core layer Substances 0.000 description 8
- 239000002131 composite material Substances 0.000 description 6
- 239000007921 spray Substances 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 239000011565 manganese chloride Substances 0.000 description 3
- 229940099607 manganese chloride Drugs 0.000 description 3
- 235000002867 manganese chloride Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000002792 vascular Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010040201 Polymyxins Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 230000005200 bud stage Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 150000003216 pyrazines Chemical class 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a microbial organic fertilizer for preventing and treating cotton wilt, which comprises the following raw materials in parts by weight: 80-120 parts of fresh chicken manure; 4-6 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 14-16 parts of egg shell powder; 9-11 parts of modified medical stone powder; 12-14 parts of konjac flour; 24-26 parts of compound microbial agent fermentation liquor; 12-13 parts of guanidine gum; 12-13 parts of sodium alginate; 12-13 parts of boric acid and 15-17 parts of calcium superphosphate; the microbial organic fertilizer can effectively prevent and treat blight in the growth cycle of cotton, and has the effect of long-acting prevention and treatment of the blight of the cotton; meanwhile, the fertilizer also contains nutrient components for promoting the growth of cotton, has the effect of promoting the growth of cotton, and can obviously improve the yield of cotton.
Description
Technical Field
The invention belongs to the field of microbial organic fertilizers, and particularly relates to a microbial organic fertilizer for preventing and treating cotton wilt and a preparation method thereof.
Background
The cotton wilt is a disease caused by fusarium oxysporum, belongs to a typical vascular bundle disease, has a risk of infection in the whole growth and development period of cotton, can cause dead seedlings of the cotton in the early period, and can cause the cotton leaves and the cotton buds to fall off or even die in the later period, and the occurrence of the wilt obviously influences the growth of the cotton, thereby causing the reduction of the yield of the cotton;
the commonly used method for preventing and treating cotton wilt at present mainly adopts chemical agents, and the chemical agents need to be frequently applied aiming at the wilt occurring in different periods, so that on one hand, a large amount of applied chemical agents have toxicity and cause environmental pollution, and on the other hand, the chemical agents can only temporarily solve a temporary disease and cannot achieve a long-term effective prevention and treatment effect;
biological control utilizes the mutual relationship among biological species, one or one type of organisms inhibits the other or another type of organisms to achieve the effect of controlling diseases, and the biological control has the characteristic of environmental friendliness, but the simple biological control microbial agent has limited control effect on plant diseases and insect pests and limited growth promotion effect on plants, and is often matched with organic fertilizers for use; and the problem of poor storage stability exists, so that research and development of a microbial organic fertilizer for preventing and treating cotton wilt for a long time are urgently needed; can not only prevent and control diseases, but also has the effect of promoting the growth of crops and improving the yield.
Disclosure of Invention
In order to solve the technical problems, the invention provides a microbial organic fertilizer for preventing and treating cotton wilt and a preparation method thereof;
the microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 80-120 parts of fresh chicken manure; 4-6 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 14-16 parts of egg shell powder; 9-11 parts of modified medical stone powder; 12-14 parts of konjac flour; 24-26 parts of compound microbial agent fermentation liquor; 12-13 parts of guanidine gum; 12-13 parts of sodium alginate; 11-13 parts of boric acid and 15-17 parts of calcium superphosphate.
Preferably, the microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 100 parts of fresh chicken manure; 5 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 15 parts of egg shell powder; 10 parts of modified medical stone powder; 13 parts of konjak powder; 25 parts of compound microbial agent fermentation liquor; 12.5 parts of guanidine gum; 12.5 parts of sodium alginate; 12 parts of boric acid and 16 parts of calcium superphosphate.
Further, the composite microorganism fermentation liquid comprises a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, a bacillus pumilus and bacillus firmus mixed fermentation liquid B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1.
Preferably, the weight ratio of the peach leaf powder to the stemona powder to the camphor powder is 2:1: 1.
Preferably, the preparation method of the modified medical stone comprises the following steps: putting medical stone into an acid solution, performing ultrasonic treatment, and washing to be neutral to obtain activated medical stone; dispersing activated Maifanitum in water solution, adding ethanol solution of gamma-glycidoxypropyltrimethoxysilane under stirring, stirring at 50 deg.C for 3 hr, filtering, washing, and drying; the modified medical stone can be obtained.
Preferably, the weight ratio of the gamma-glycidoxypropyltrimethoxysilane to the medical stone is 1: 5; the weight ratio of the gamma-glycidoxypropyltrimethoxysilane to the ethanol in the ethanol solution of the gamma-glycidoxypropyltrimethoxysilane is 1: 1.
Further, the preparation method of the composite microbial fermentation liquid specifically comprises the following steps:
1) respectively inoculating paenibacillus polymyxa, bacillus amyloliquefaciens, bacillus pumilus and bacillus firmus into a slant culture medium to culture so as to obtain activated seed liquid for later use;
2) mixing and transferring seed liquid of the paenibacillus polymyxa and the bacillus amyloliquefaciens into a fermentation tank, and performing fermentation culture to obtain mixed fermentation bacteria liquid of the paenibacillus polymyxa and the bacillus amyloliquefaciens, wherein the inoculation amount of the seed liquid is 5% of the total fermentation volume; the weight ratio of the paenibacillus polymyxa to the bacillus amyloliquefaciens in the mixed seed liquid is 1: 1;
3) mixing seed solutions of the bacillus firmus and the bacillus pumilus, transferring the seed solutions into a fermentation tank, and performing fermentation culture to obtain a bacillus firmus and bacillus pumilus fermented solution; the inoculation amount of the seed liquid is 5% of the total fermentation volume; the weight ratio of the bacillus firmus to the bacillus pumilus seed liquid is 1: 1;
the culture medium of the bacillus amyloliquefaciens and the paenibacillus polymyxa is as follows: 18g/L of corn flour, 15g/L of peptone, 15g/L of yeast powder, 1g/L of sodium chloride, 0.03g/L of manganese chloride and 1g/L of monopotassium phosphate, wherein the pH value is 7.0; culturing at 31-37 deg.C for 12-24 hr;
the culture medium of the bacillus pumilus and the bacillus firmus is as follows: 18g/L of corn flour, 15g/L of peptone, 15g/L of yeast powder, 1g/L of sodium chloride, 0.03g/L of manganese chloride and 1g/L of monopotassium phosphate, wherein the pH value is 8.0, and the culture temperature is 28-30 ℃; culturing for 12-24 h.
Further, the preparation method of the microbial organic fertilizer for preventing and treating cotton wilt comprises the following steps:
(1) chicken manure pretreatment: adding peach leaf powder and stemona powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and carrying out freeze drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding half of the egg shell powder in part by weight into the chicken manure powder in the step (1), uniformly stirring, adding modified medical stone powder and konjac powder, uniformly mixing, adding a bacillus firmus and bacillus pumilus mixed fermentation liquor, adding water to adjust the water content of a system to be 60-70%, fermenting for 2 days, adding the rest egg shell powder, uniformly stirring, adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquor, and fermenting for 5 days to obtain a fermentation product;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) and (4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying the mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product.
Preferably, the coating solution comprises a coating solution 1 and a coating solution 2, wherein the coating solution 1 is a mixed solution of guanidine gum and sodium alginate, the coating solution 2 is a boric acid solution, and the coating treatment is to spray the coating solution 1 and the coating solution 2 simultaneously.
Preferably, the mass concentration of the guanidine gum in the coating liquid is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the mass concentration of the boric acid solution is 1 wt%.
Advantageous effects
The microbial organic fertilizer for preventing and treating cotton wilt can effectively prevent and treat cotton wilt in the growth cycle, and has the effect of long-acting prevention and treatment of cotton wilt; meanwhile, the fertilizer also contains nutrient components for promoting the growth of cotton, has the effect of promoting the growth of cotton, and can obviously improve the yield of cotton. The bio-organic fertilizer has the double effects of preventing and treating cotton wilt and improving cotton yield, and comprises a core layer and a coating layer; the core layer and the coating layer both contain effective components for preventing and treating cotton wilt, and the effective components achieve the effect of slow release and can prevent and treat diseases of cotton in different growth stages.
The microbial organic fertilizer comprises a core layer and a coating layer, wherein the core layer comprises a fermentation product obtained by sequentially fermenting a mixture of chicken manure, konjac flour and egg shell powder by using a bacillus firmus and bacillus pumilus mixed strain fermentation liquid and fermenting a paenibacillus polymyxa and bacillus amyloliquefaciens mixed strain fermentation liquid; before fermentation, peach leaf powder, stemona powder and camphor powder are added into fresh chicken manure, so that the effect of preliminarily killing protobacteria and worm eggs in the chicken manure can be achieved; the later-stage compound microorganism does not need to be subjected to a high-temperature fermentation process; according to the invention, bacillus firmus and bacillus pumilus are firstly used for fermentation, and then paenibacillus polymyxa and bacillus amyloliquefaciens are added for fermentation treatment, so that the decomposition effect of the chicken manure is obviously improved; the konjac flour is added into the fermentation system, so that the viscosity of the fermentation product is improved, and the later-period fertilizer core particle forming is facilitated; the coating layer is formed by molding guanidine gum and sodium alginate under boric acid as a cross-linking agent, and meanwhile, calcium ions in the core layer are easy to combine with the coating components, so that the stability of the core layer and the coating layer in the fertilizer is obviously improved, and the yield of the fertilizer is improved;
boric acid is selected as a cross-linking agent for the coating layer of the bacterial fertilizer, and the outer coating layer is degraded in the early growth stage of cotton, wherein boron is beneficial to the growth of cotton vascular bundle tissues, so that the conveying of other nutrient elements is promoted, and the blight caused by vascular bundle erosion caused by pathogenic bacteria in the early stage is reduced; along with the degradation of the outer coating layer, nutrient elements required for the growth of microorganisms in the core layer are provided, the microorganisms in the core layer are gradually propagated at a fixed value at the root of the cotton, and can secrete extracellular polysaccharide, polymyxin, peptides, protease, pyrazines, phenols and other antibacterial substances in the microbial propagation process, and meanwhile, a biological film can be formed at the root of the cotton; the microorganisms protect cotton from being invaded by pathogens through the action modes of site competition, induced resistance and the like.
Experiments prove that the chicken manure has better prevention and treatment effect on cotton wilt compared with the biological organic fertilizer of other animal manure.
Detailed Description
The technical solution of the present invention is described in detail with reference to the specific embodiments, but it should be emphasized that the present invention is not limited to the specific embodiments; the microbial species used in the examples below can be isolated by themselves or obtained by purchase; the starting materials described in the examples below are, without specific indication, either prepared by conventional methods or commercially available.
Example 1
Preparation of modified medical stone
(1) Pretreatment of medical stone: 200-mesh and 300-mesh medical stone powder is placed in a hydrochloric acid solution for ultrasonic treatment for 30-60min at 300-mesh and 400W, and washed until washing liquid is neutral and dried to obtain activated medical stone;
(2) dispersing 10kg of activated medical stone powder in an aqueous solution, dropwise adding ammonia water to adjust the pH of the system to be 8-9, adding 4kg of an ethanol solution of gamma-glycidoxypropyltrimethoxysilane (the weight ratio of the gamma-glycidoxypropyltrimethoxysilane to the ethanol is 1:1) under the condition of stirring, stirring at 50 ℃ for 3 hours, filtering, washing and drying; the modified medical stone can be obtained.
Example 2
Preparation of microbial fermentation broth
Respectively inoculating paenibacillus polymyxa, bacillus amyloliquefaciens, bacillus pumilus and bacillus firmus into a culture medium to be cultured to obtain activated seed liquid for later use;
mixing and transferring seed liquid of paenibacillus polymyxa and bacillus amyloliquefaciens into a fermentation tank, and performing fermentation culture to obtain mixed fermentation bacterium liquid A of the paenibacillus polymyxa and the bacillus amyloliquefaciens, wherein the inoculation amount of the seed liquid is 5% of the total volume of the fermentation tank; the weight ratio of the paenibacillus polymyxa seed liquid to the bacillus amyloliquefaciens seed liquid in the mixed seed liquid is 1: 1;
mixing seed solutions of the bacillus firmus and the bacillus pumilus, transferring the seed solutions into a fermentation tank, and performing fermentation culture to obtain a bacillus firmus and bacillus pumilus fermented solution B; the inoculation amount of the seed liquid is 5 percent of the total volume of the fermentation tank; the weight ratio of the bacillus firmus to the bacillus pumilus seed liquid is 1: 1;
the culture medium of the bacillus amyloliquefaciens and the paenibacillus polymyxa is as follows: corn flour, peptone, yeast powder, sodium chloride and potassium dihydrogen phosphate, wherein the pH value is 7.0; culturing at 31-37 deg.C for 12-24 hr;
the culture medium of the bacillus pumilus and the bacillus firmus is as follows: corn flour, peptone, yeast powder, sodium chloride, manganese chloride, potassium dihydrogen phosphate and calcium chloride, wherein the pH value is 8.0, and the culture temperature is 28-30 ℃; culturing for 12-24 h.
Example 3
A microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 80 parts of fresh chicken manure; 4 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 14 parts of egg shell powder; 9 parts of modified medical stone powder; 12 parts of konjak powder; 24 parts of compound microorganism fermentation liquor; 12 parts of guanidine gum; 12 parts of sodium alginate; 11 parts of boric acid and 15 parts of calcium superphosphate;
the composite microorganism fermentation liquid comprises a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, a bacillus pumilus and bacillus firmus mixed fermentation liquid B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1;
the weight ratio of the peach leaf powder to the stemona powder to the camphor powder is 2:1: 1;
the preparation method of the microbial organic fertilizer for preventing and treating cotton wilt comprises the following steps:
(1) chicken manure pretreatment: adding peach leaf powder and stemona powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and carrying out freeze drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding half of the egg shell powder in the step (1) by weight into the chicken manure powder, uniformly stirring, adding modified medical stone powder (prepared in example 1) and konjac flour, uniformly mixing, adding a bacillus firmus and bacillus pumilus mixed fermentation liquor B, adding water to adjust the water content of the system to be 60-70%, fermenting for 2 days, adding the rest egg shell powder, uniformly stirring, adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquor A, and fermenting for 5 days to obtain a fermentation product; wherein, the mixed fermentation liquor A and the mixed fermentation liquor B are both obtained in the embodiment 2;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying a mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product;
the coating solution comprises a coating solution 1 and a coating solution 2, wherein the coating solution 1 is a mixed solution of guanidine gum and sodium alginate, and the mass concentration of the guanidine gum is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the coating liquid 2 is boric acid solution with the mass concentration of 1wt%, and the coating treatment is to spray the coating liquid 1 and the coating liquid 2 at the same time.
Example 4
A microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 100 parts of fresh chicken manure; 5 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 15 parts of egg shell powder; 10 parts of modified medical stone powder; 13 parts of konjac flour and 25 parts of compound microbial agent fermentation liquor; 16 parts of calcium superphosphate; 12.5 parts of guanidine gum; 12.5 parts of sodium alginate; 12 parts of boric acid; the composite microorganism fermentation liquid comprises a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, a bacillus pumilus and bacillus firmus mixed fermentation liquid B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1;
the weight ratio of the peach leaf powder to the stemona powder to the camphor powder is 2:1: 1;
the preparation method of the microbial organic fertilizer for preventing and treating cotton wilt comprises the following steps:
(1) chicken manure pretreatment: adding peach leaf powder and radix stemonae powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and performing freeze drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding half of the egg shell powder in part by weight into the chicken manure powder in the step (1), uniformly stirring, adding modified medical stone powder (prepared in example 1) and konjac flour, uniformly mixing, adding a bacillus firmus and bacillus pumilus mixed fermentation liquid B, adding water to adjust the water content of the system to be 60-70%, fermenting for 2 days, adding the rest egg shell powder, uniformly stirring, adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, and fermenting for 5 days to obtain a fermentation product; wherein, the mixed fermentation liquor A and the mixed fermentation liquor B are both obtained in the embodiment 2;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying a mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product;
the coating solution comprises a coating solution 1 and a coating solution 2, wherein the coating solution 1 is a mixed solution of guanidine gum and sodium alginate, and the mass concentration of the guanidine gum is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the coating liquid 2 is boric acid solution with the mass concentration of 1wt%, and the coating treatment is to spray the coating liquid 1 and the coating liquid 2 at the same time.
Example 5
A microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 120 parts of fresh chicken manure; 6 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 16 parts of egg shell powder; 11 parts of modified medical stone powder; 14 parts of konjac flour and 26 parts of compound microbial agent fermentation liquor; 17 parts of calcium superphosphate; 13 parts of guanidine gum; 13 parts of sodium alginate; 13 parts of boric acid;
the composite microorganism fermentation liquid comprises a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, a bacillus pumilus and bacillus firmus mixed fermentation liquid B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1;
the weight ratio of the peach leaf powder to the radix stemonae powder is 2: 1;
the preparation method of the microbial organic fertilizer for preventing and treating cotton wilt comprises the following steps:
(1) chicken manure pretreatment: adding the mixture of peach leaf powder, stemona powder and camphor powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and freeze-drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding half of the egg shell powder in the step (1) by weight into the chicken manure powder, uniformly stirring, adding modified medical stone powder (prepared in example 1) and konjac flour, uniformly mixing, adding a bacillus firmus and bacillus pumilus mixed fermentation liquor B, adding water to adjust the water content of the system to be 60-70%, fermenting for 2 days, adding the rest egg shell powder, uniformly stirring, adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquor A, and fermenting for 5 days to obtain a fermentation product; wherein, the mixed fermentation liquor A and the mixed fermentation liquor B are both obtained in the embodiment 2;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying a mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product;
the coating solution comprises a coating solution 1 and a coating solution 2, wherein the coating solution 1 is a mixed solution of guanidine gum and sodium alginate, and the mass concentration of the guanidine gum is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the coating liquid 2 is boric acid solution with the mass concentration of 1wt%, and the coating treatment is to spray the coating liquid 1 and the coating liquid 2 at the same time.
Comparative example 1
The preparation method of the microbial organic fertilizer in the comparative example 1 is basically the same as that of the microbial organic fertilizer in the example 4, except that the enveloping treatment in the step (4) is carried out by only adopting the enveloping liquid 1.
Comparative example 2
Comparative example 2 the microbial organic fertilizer prepared in example 4 was prepared in substantially the same manner except that fresh chicken manure was replaced with fresh pig manure in the same amount.
Comparative example 3
A microbial organic fertilizer for preventing and treating cotton wilt comprises the following raw materials in parts by weight: 100 parts of fresh chicken manure; 5 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 15 parts of egg shell powder; 10 parts of modified medical stone powder; 13 parts of konjac flour and 25 parts of compound microbial agent fermentation liquor; 16 parts of calcium superphosphate; 12.5 parts of guanidine gum; 12.5 parts of sodium alginate; 12 parts of boric acid; the composite microorganism fermentation liquid comprises a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquid A, a bacillus pumilus and bacillus firmus mixed fermentation liquid B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1;
the weight ratio of the peach leaf powder to the stemona powder to the camphor powder is 2:1: 1;
the preparation method of the microbial organic fertilizer for preventing and treating cotton wilt comprises the following steps:
(1) chicken manure pretreatment: adding the mixture of peach leaf powder, stemona powder and camphor powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and freeze-drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding the egg shell powder in the weight part into the chicken manure powder in the step (1), uniformly stirring, then adding the modified medical stone powder (prepared in example 1) and the konjac flour, uniformly mixing, then adding a bacillus firmus and bacillus pumilus mixed fermentation liquor B, then adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquor A, uniformly stirring, then adding water to adjust the water content of the system to be 60-70%, and fermenting for 7 days to obtain a fermentation product; wherein the mixed fermentation broth a and the mixed fermentation broth B were both obtained in example 2;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying a mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product;
the coating solution comprises a coating solution 1 and a coating solution 2, wherein the coating solution 1 is a mixed solution of guanidine gum and sodium alginate, and the mass concentration of the guanidine gum is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the coating liquid 2 is boric acid solution with the mass concentration of 1wt%, and the coating treatment is to spray the coating liquid 1 and the coating liquid 2 at the same time.
Evaluation of prevention and treatment effect of microbial organic fertilizer on cotton wilt
The microbial organic fertilizer prepared in the embodiment and the proportion of the invention is used as a base fertilizer for cotton planting, and the influence of the organic fertilizer on the cotton growth condition is examined. Wherein the cotton variety is medium cotton 63;
the test field is selected from a cotton planting field with high blight in Shangqiu city, and the test base fertilizer is selected from microbial organic fertilizers prepared in the embodiment 4 and the comparative examples 1-3 of the invention;
the treatment method comprises the following steps of dividing the treatment method into an experimental group and a control group, wherein the control group adopts a local conventional base fertilizer; the experimental components are 4 groups, and the microbial organic fertilizer of the embodiment 4 and the microbial organic fertilizers of the comparative examples 1-3 are respectively applied as base fertilizers; the control group and the test group adopt the same field management during the cotton growth period, the growth condition of the cotton is regularly observed until the cotton is harvested, and the cotton grows for 60 days under the seed; the cotton grows into the third stage, namely the bud stage, which is the first incidence peak stage of blight of the cotton; at the moment, the morbidity of the cotton in each group of plots is counted, after the cotton is harvested, the yield of the cotton in each group is counted, and the result is recorded in table 1; wherein, observation shows that the cotton in the plot applied with the organic fertilizer in the comparative example 3 has seedling burning phenomenon; thus, the group of comparative example 3 was rejected when the incidence and yield were statistically recorded.
Table 1.
As can be seen from the data in Table 1, the microbial organic fertilizer obtained in the embodiment 4 of the invention can effectively prevent and treat cotton wilt, reduce the incidence rate of the cotton wilt and simultaneously can obviously improve the yield of cotton, while the microbial organic fertilizers in the comparative examples 1 and 2 also have certain prevention and treatment effects on the wilt, but the prevention and treatment effects on the wilt are lower than those of the embodiment 4, which shows that boric acid as a cross-linking agent of a coating layer can improve the prevention and treatment effects on the wilt of the microbial organic fertilizer; also indicates that the prevention and treatment effect of the organic fertilizer prepared by using the pig manure as a microbial carrier on cotton wilt is weaker than that of chicken manure.
The above experimental examples are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any simple changes or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention are within the scope of the present invention.
Claims (10)
1. A microbial organic fertilizer for preventing and treating cotton wilt is characterized by comprising the following raw materials in parts by weight: 80-120 parts of fresh chicken manure; 4-6 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 14-16 parts of egg shell powder; 9-11 parts of modified medical stone powder; 12-14 parts of konjac flour; 24-26 parts of compound microorganism fermentation liquor; 12-13 parts of guanidine gum; 11-13 parts of sodium alginate; 11-13 parts of boric acid and 15-17 parts of calcium superphosphate.
2. The microbial organic fertilizer for preventing and treating cotton wilt as claimed in claim 1, which comprises the following raw materials in parts by weight: 100 parts of fresh chicken manure; 5 parts of a mixture of peach leaf powder, radix stemonae powder and camphor powder; 15 parts of egg shell powder; 10 parts of modified medical stone powder; 13 parts of konjak powder; 25 parts of compound microbial agent fermentation liquor; 12.5 parts of guanidine gum; 12.5 parts of sodium alginate; 12 parts of boric acid and 16 parts of calcium superphosphate.
3. The microbial organic fertilizer for controlling cotton wilt according to claim 1, wherein said complex microbial fermentation broth comprises Paenibacillus polymyxa and Bacillus amyloliquefaciens mixed fermentation broth A, Bacillus pumilus and Bacillus firmus mixed fermentation broth B; the weight ratio of the mixed fermentation liquor A to the mixed fermentation liquor B is 1: 1.
4. The microbial organic fertilizer for preventing and treating cotton wilt as claimed in claim 1, wherein the weight ratio of peach leaf powder, radix stemonae powder and camphor powder is 2:1: 1.
5. The microbial organic fertilizer for preventing and treating cotton wilt as claimed in claim 1, wherein the preparation method of the modified medical stone comprises the following steps: putting medical stone into an acid solution, performing ultrasonic treatment, and washing to be neutral to obtain activated medical stone; dispersing activated Maifanitum in water solution, adding ethanol solution of gamma-glycidoxypropyltrimethoxysilane under stirring, stirring at 50 deg.C for 3 hr, filtering, washing, and drying; the modified medical stone can be obtained.
6. The microbial organic fertilizer for preventing and treating cotton wilt as claimed in claim 5, wherein the weight ratio of gamma-glycidoxypropyltrimethoxysilane to Maifanitum is 1: 5; the weight ratio of the gamma-glycidoxypropyltrimethoxysilane to the ethanol in the ethanol solution of the gamma-glycidoxypropyltrimethoxysilane is 1: 1.
7. The microbial organic fertilizer for preventing and treating cotton wilt disease of claim 3, wherein the preparation method of the compound microbial fermentation liquid comprises the following steps:
1) respectively inoculating paenibacillus polymyxa, bacillus amyloliquefaciens, bacillus pumilus and bacillus firmus into a slant culture medium to culture so as to obtain activated seed liquid for later use;
2) mixing and transferring seed liquid of the paenibacillus polymyxa and the bacillus amyloliquefaciens into a fermentation tank, and performing fermentation culture to obtain a mixed fermentation bacterium liquid A of the paenibacillus polymyxa and the bacillus amyloliquefaciens, wherein the inoculation amount of the seed liquid is 5% of the total fermentation volume; the weight ratio of the paenibacillus polymyxa to the bacillus amyloliquefaciens in the mixed seed liquid is 1: 1;
3) mixing seed solutions of the bacillus firmus and the bacillus pumilus, transferring the seed solutions into a fermentation tank, and performing fermentation culture to obtain a bacillus firmus and bacillus pumilus fermented solution B; the inoculation amount of the seed liquid is 5% of the total fermentation volume; the weight ratio of the bacillus firmus to the bacillus pumilus seed liquid is 1: 1.
8. The preparation method of the microbial organic fertilizer for preventing and treating cotton wilt as claimed in claim 1, which comprises the following steps:
(1) chicken manure pretreatment: adding the mixture of peach leaf powder, stemona powder and camphor powder into the fresh chicken manure in parts by weight, uniformly stirring, standing for 2 days, and freeze-drying and crushing to obtain chicken manure powder;
(2) fermentation of chicken manure: adding half of the egg shell powder in the weight part into the chicken manure powder in the step (1), uniformly stirring, adding modified medical stone powder and konjac powder, uniformly mixing, adding a bacillus firmus and bacillus pumilus mixed fermentation liquor, adding water to adjust the water content of a system to be 60-70%, fermenting for 2 days, adding the rest egg shell powder, uniformly stirring, adding a paenibacillus polymyxa and bacillus amyloliquefaciens mixed fermentation liquor, and fermenting for 5 days to obtain a fermentation product;
(3) adding calcium superphosphate into the fermentation product obtained in the step (2), uniformly stirring, feeding into an extrusion granulator, and granulating to obtain fertilizer core particles;
(4) and (4) pouring the fertilizer core particles obtained in the step (3) into a horizontal roller, and spraying the mist coating liquid into the horizontal roller at the same time until the fertilizer core is completely and uniformly coated by the coating layer to obtain a finished product.
9. The preparation method of the microbial organic fertilizer for preventing and treating cotton wilt according to claim 8, wherein said coating solution comprises coating solution 1 and coating solution 2, said coating solution 1 is a mixed solution of guanidine gum and sodium alginate, said coating solution 2 is a boric acid solution, and said coating treatment is spraying coating solution 1 and coating solution 2 simultaneously.
10. The preparation method of the microbial organic fertilizer for preventing and treating cotton wilt according to claim 9, wherein the mass concentration of guanidine gum in the coating liquid 1 is 1 wt%; the mass concentration of the sodium alginate is 1 wt%; the mass concentration of the boric acid solution is 1 wt%.
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