CN114424775A - Compound microorganism wettable powder for preventing and treating nematodes and preparation method and application thereof - Google Patents
Compound microorganism wettable powder for preventing and treating nematodes and preparation method and application thereof Download PDFInfo
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- CN114424775A CN114424775A CN202210011877.5A CN202210011877A CN114424775A CN 114424775 A CN114424775 A CN 114424775A CN 202210011877 A CN202210011877 A CN 202210011877A CN 114424775 A CN114424775 A CN 114424775A
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- paecilomyces lilacinus
- penicillium oxalicum
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- 239000004563 wettable powder Substances 0.000 title claims abstract description 35
- 150000001875 compounds Chemical class 0.000 title claims abstract description 28
- 241000244206 Nematoda Species 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 244000005700 microbiome Species 0.000 title claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 40
- 241001465752 Purpureocillium lilacinum Species 0.000 claims abstract description 36
- 241000985513 Penicillium oxalicum Species 0.000 claims abstract description 34
- 230000000813 microbial effect Effects 0.000 claims abstract description 32
- 238000004321 preservation Methods 0.000 claims abstract description 23
- 239000005995 Aluminium silicate Substances 0.000 claims abstract description 8
- 235000012211 aluminium silicate Nutrition 0.000 claims abstract description 8
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 8
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229920005552 sodium lignosulfonate Polymers 0.000 claims abstract description 8
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 230000008635 plant growth Effects 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 18
- 230000004151 fermentation Effects 0.000 claims description 18
- 239000001963 growth medium Substances 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 239000002131 composite material Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002270 dispersing agent Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 230000009471 action Effects 0.000 abstract description 4
- 230000000295 complement effect Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000011282 treatment Methods 0.000 description 14
- 239000002689 soil Substances 0.000 description 7
- 235000002566 Capsicum Nutrition 0.000 description 6
- 229920000742 Cotton Polymers 0.000 description 6
- 241000219146 Gossypium Species 0.000 description 6
- 239000006002 Pepper Substances 0.000 description 6
- 241000722363 Piper Species 0.000 description 6
- 235000016761 Piper aduncum Nutrition 0.000 description 6
- 235000017804 Piper guineense Nutrition 0.000 description 6
- 235000008184 Piper nigrum Nutrition 0.000 description 6
- 230000012010 growth Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 241000813090 Rhizoctonia solani Species 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 235000000832 Ayote Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000015136 pumpkin Nutrition 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000219122 Cucurbita Species 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000001069 nematicidal effect Effects 0.000 description 1
- 239000005645 nematicide Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/36—Penicillium
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a compound microorganism wettable powder for preventing and treating nematodes and a preparation method and application thereof, wherein the powder consists of effective components and an auxiliary agent, and the effective components are as follows: the paecilomyces lilacinus powder is obtained by fermenting paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761, and the penicillium oxalicum powder is obtained by fermenting penicillium oxalicum WZ006 with the preservation number of CGMCC No. 13763; the auxiliary agent is: kaolin, a diffusant NNO and sodium lignosulfonate. The invention has the advantages that: (1) the two microbial inocula complement each other in action, so that the nematode can be effectively prevented and treated; (2) the affinity is good, and the survival and the stability of spores are ensured; (3) can prevent and treat soil-borne diseases; (4) can promote plant growth.
Description
The application is a divisional application with the application date of 04.05.2017 and the application number of 201710306254.X, and the invention name of composite microbial wettable powder for preventing and treating nematodes and a preparation method thereof.
Technical Field
The invention relates to a microbial preparation and a preparation method thereof, in particular to a compound microbial wettable powder for preventing and treating nematodes as well as a preparation method and application thereof, belonging to the technical field of agricultural microorganisms.
Background
In recent years, due to continuous cropping area expansion and unscientific application of medicines, nematode development and spread speed is high, resistance is enhanced, and crop seedling death rate is increased, so that crop yield is reduced or even dead crop is produced, quality is reduced, and crop production is seriously influenced.
Various nematocides for preventing and treating nematodes exist in the market, but the problems of difficult transportation and storage, short shelf life and the like are caused by single strain, incompatibility of auxiliary agents and the like.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the compound microorganism wettable powder for preventing and treating the nematodes as well as the preparation method and the application thereof.
In order to achieve the above object, the present invention adopts the following technical solutions:
the invention provides a compound microorganism wettable powder for preventing and treating nematode, which consists of effective components and an auxiliary agent,
the effective components are as follows: the paecilomyces lilacinus powder is obtained by fermenting paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761, and the penicillium oxalicum powder is obtained by fermenting penicillium oxalicum WZ006 with the preservation number of CGMCC No. 13763;
the auxiliary agent is as follows: kaolin, a diffusant NNO and sodium lignosulfonate.
Preferably, the spore content of the paecilomyces lilacinus powder is more than or equal to 120 hundred million/g, and the spore content of the penicillium oxalicum powder is more than or equal to 120 hundred million/g.
Preferably, the content of each component is as follows by mass percent:
preferably, the preparation method is as follows:
step 1: respectively inoculating paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761 and penicillium oxalicum WZ006 with the preservation number of CGMCC No.13763 on a PDA plate medium, culturing at 27 ℃ in the dark for 7 days, and taking the cultured plate as an original strain;
step 2: respectively inoculating the stock seeds cultured by Step1 on a PDA liquid culture medium, culturing at 27 ℃ in the dark at 121rpm for 4d, and taking the fermented liquid as a seed liquid;
step 3: adding water into testa Tritici, controlling humidity at 20%, and sterilizing at 121 deg.C for 30min to obtain fermentation culture medium;
step 4: respectively adding the seed liquid cultured in Step2 into the fermentation culture medium prepared in Step3, and standing and dark culturing at 27 ℃ for 14 d;
step 5: respectively drying and crushing fermentation liquor prepared by Step4, and sieving the fermentation liquor with a 100-mesh sieve to obtain paecilomyces lilacinus powder with spore content of more than or equal to 120 hundred million/g and penicillium oxalicum powder with spore content of more than or equal to 120 hundred million/g;
step 6: mixing the paecilomyces lilacinus powder and the penicillium oxalicum powder prepared by Step5 with kaolin, a dispersing agent NNO and sodium lignosulphonate uniformly to obtain the paecilomyces lilacinus powder.
The invention provides application of the compound microbial wettable powder in the technical scheme in prevention and treatment of nematodes.
The invention provides application of the compound microbial wettable powder in the technical scheme in prevention and treatment of soil-borne diseases.
The invention provides application of the composite microbial wettable powder in the technical scheme in phosphate and potassium dissolution.
The invention provides application of the compound microbial wettable powder in the technical scheme in promoting plant growth.
The invention has the advantages that:
(1) the compound microbial wettable powder contains paecilomyces lilacinus and penicillium oxalicum at the same time, and the two bactericides complement each other in action, so that nematodes can be effectively prevented and treated; kaolin, a dispersing agent NNO and sodium lignosulfonate are selected as auxiliary agents, so that the compatibility is good, the survival and the stability of spores are guaranteed, the storage time is prolonged, and the transportation and storage cost is reduced;
(2) according to the compound microorganism wettable powder, paecilomyces lilacinus can parasitize nematode eggs, secondary metabolites are secreted to inhibit the growth of the nematodes, and the penicillium oxalicum can prevent and control soil-borne diseases while preventing and controlling the nematodes;
(3) the penicillium oxalicum has the functions of dissolving phosphorus and potassium, and can promote plant growth.
Biological preservation information
Paecilomyces lilacinus (Paecilomyces lilacinus) WZ004 is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of Xilu No.1 of Beijing, Chaoyang, the preservation date is 03 and 14 days in 2017, and the preservation number is CGMCC No. 13761.
The Penicillium oxalicum (Penicillium oxalicum) WZ006 is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of West Lu No.1 of Beijing, Chaoyang, the preservation date is 2017, 03 and 14 days, and the preservation number is CGMCC No. 13763.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
A first part: preparation of Paecilomyces lilacinus powder
1. Screening of isolated strains
Diluting 10 the soil collected by Shandong Shouguang greenhouse-5Uniformly spreading on PDA culture medium containing ampicillin, separating and culturing, and selecting single fungus colony。
2. Identification of strains
And (3) carrying out molecular identification by using the ITS sequence, and selecting a paecilomyces lilacinus strain.
3. Strain preservation
The screened and separated paecilomyces lilacinus WZ004 is preserved in China general microbiological culture Collection center (No. 3 of Xilu No.1 of Beijing Kogyo Chaoyang district), with the preservation number of CGMCC No.13761, in 2017, 03 and 14 months.
4. Culturing stock
The paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761 is inoculated on a PDA plate culture medium, dark culture is carried out for 7d at the temperature of 27 ℃, and the cultured plate is used as an original strain.
5. Seed culture solution
Inoculating the cultured stock seed on PDA liquid culture medium, culturing at 27 deg.C under dark condition at 121rpm for 4d, and collecting the fermented liquid as seed liquid.
6. Preparation of fermentation Medium
Adding water into testa Tritici, controlling humidity at 20%, and sterilizing at 121 deg.C for 30min to obtain fermentation culture medium.
7. Fermentation of
Adding the seed liquid into the above obtained testa Tritici fermentation medium, and standing at 27 deg.C for 14 d.
8. Making into powder
Drying the fermentation liquor, crushing and sieving by a 100-mesh sieve to obtain the paecilomyces lilacinus powder.
The detection proves that the content of spores in the paecilomyces lilacinus powder is 300 hundred million/g.
A second part: preparation of Penicillium oxalicum powder
1. Screening of isolated strains
Diluting 10 the soil collected by Shandong Shouguang greenhouse-5The culture medium is evenly spread on a PDA culture medium containing ampicillin for isolated culture, and a single fungus colony is selected.
2. Identification of strains
And (4) performing molecular identification by using the ITS sequence, and selecting the penicillium oxalicum strain.
3. Strain preservation
The screened and separated penicillium oxalicum WZ006 is preserved in China general microbiological culture Collection center (No. 3 of Xilu-1. Beijing, Chaoyang) in 2017, 03 and 14 days, and the preservation number is CGMCC No. 13763.
4. Culturing stock
The penicillium oxalicum WZ006 with the preservation number of CGMCC No.13763 is inoculated on a PDA plate culture medium, and is cultured in dark at 27 ℃ for 7d, and the cultured plate is used as an original strain.
5. Seed culture solution
Inoculating the cultured stock onto PDA liquid culture medium, culturing at 27 deg.C under dark condition at 121rpm for 4d, and collecting the fermented liquid as seed liquid.
6. Preparation of fermentation Medium
Adding water into testa Tritici, controlling humidity at 20%, and sterilizing at 121 deg.C for 30min to obtain fermentation culture medium.
7. Fermentation of
Adding the seed liquid into the above obtained testa Tritici fermentation medium, and standing at 27 deg.C for 14 d.
8. Making into powder
Drying the fermentation liquor, crushing and sieving by a 100-mesh sieve to obtain the penicillium oxalicum powder.
Through detection, the content of spores in the penicillium oxalicum powder is 400 hundred million/g.
And a third part: preparation of composite microbial wettable powder
And uniformly mixing the paecilomyces lilacinus powder prepared by the first part and the penicillium oxalicum powder prepared by the second part with kaolin, a diffusant NNO and sodium lignosulphonate to obtain the compound microbial wettable powder.
The content of each component is as follows by mass percent:
the fourth part: performance evaluation of Compound microbial wettable powder
1. Nematode control effect
In a pumpkin greenhouse of Changle in Shandong, four acres of land are selected, 1kg of the compound microbial agent is used for one acre of land, 1kg of paecilomyces lilacinus powder is used for one acre of land, and 1kg of the penicillium oxalicum powder is used for one acre of land, the three groups are used as experimental groups, the application method is to perform flushing application on root soil, no treatment is performed on one acre of land, and the group is used as a control group. The comparison results are shown in table 1:
TABLE 1 greenhouse nematode control of certain pumpkins from Shandong Changle
In a certain cucumber greenhouse of Shandong Shouguang, four acres of land are selected, 1kg of the compound microbial agent is used for one acre of land, 1kg of the paecilomyces lilacinus powder is used for one acre of land, and 1kg of the penicillium oxalicum powder is used for one acre of land, the three groups are used as experimental groups, the application method is to mix soil with the root soil for application, no treatment is used for one acre of land, and the group is used as a control group. The comparison results are shown in table 2:
TABLE 2 control of nematodes in greenhouse of Shandong Shouguang cucumber
It can be seen from this that: in the composite microbial wettable powder, the action of the paecilomyces lilacinus and the action of the penicillium oxalicum are complementary, so that the nematode can be effectively prevented and treated.
2. Compatibility of the powder
Mixing the fermented paecilomyces lilacinus and the fermented penicillium oxalicum in proportion to obtain the compound microbial wettable powder with the spore content of 150 hundred million/g paecilomyces lilacinus and 160 hundred million/g penicillium oxalicum. The obtained compound microbial wettable powder is placed in a shade place for 12 months and 10 days in 2015 to 10 months and 12 months in 2016 for 10 days. The microbial inoculum placed in 2016, 12, 10 days is re-diluted and plated, the number of viable bacteria is detected, and the detection result is shown in table 3:
TABLE 3 viable count of Paecilomyces lilacinus and Paecilomyces oxalicum
| Number of viable bacteria | |
| Paecilomyces lilacinus | 130 hundred million/gram |
| Penicillium oxalicum | 140 hundred million/gram |
It can be seen from this that: the compound microbial wettable powder has good affinity, ensures the survival and stability of spores and prolongs the storage time.
3. Other effects
(1) Prevention and control of soil-borne diseases
The test was carried out under greenhouse conditions (25 ℃) and the pots were filled with sterilized soil for use.
The experiment was set up with 5 treatments:
treatment 1: firstly, cotton seedlings are sown, the soil is irrigated by rhizoctonia solani suspension, and then the hydrosolvent of the composite microbial wettable powder is irrigated.
And (3) treatment 2: cotton seedlings are sown firstly, the hydrosolvent of the composite microbial wettable powder is irrigated, and then the rhizoctonia solani suspension is irrigated.
And (3) treatment: firstly, cotton seedlings are sown and watered with the rhizoctonia solani suspension.
And (4) treatment: firstly, cotton seedlings are sown and watered with the hydrosolvent of the composite microbial wettable powder.
And (4) treatment 5: cotton seedlings were sown first and watered with water as a control.
Each 10 pots were treated, each pot was sown with one seedling, and the test was repeated four times.
The morbidity is detected, and the detection result is shown in a table 4:
TABLE 4 Cotton seedling morbidity
| Incidence of disease | |
| Process 1 | 2％ |
| Treatment 2 | 1％ |
| Treatment 3 | 76％ |
| Treatment 4 | 0 |
| Treatment 5 | 0 |
It can be seen from this that: the composite microbial wettable powder can prevent and treat nematode and soil-borne diseases.
(2) Promoting plant growth
The compound microbial wettable powder is uniformly mixed with water, pepper seedlings in one mu of land are subjected to flushing application, and the growth vigor and the yield of the pepper seedlings are investigated.
And (4) setting a control group, irrigating one mu of pepper seedlings with clear water, and investigating the growth vigor and yield of the pepper seedlings.
The growth vigor and yield of pepper seedlings were investigated as shown in table 5:
TABLE 5 growth vigor and yield of pepper seedlings
| Experimental group (average) | Control group (average) | |
| Plant height | 48cm | 36cm |
| Yield of the product | 0.80 kg/strain | 0.38 kg/strain |
It can be seen from this that: the compound microbial wettable powder of the invention has the function of promoting plant growth while preventing and treating nematodes.
It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the protection scope of the present invention.
Claims (9)
1. A compound microorganism wettable powder for preventing and treating nematode is characterized in that the effective components comprise paecilomyces lilacinus powder and penicillium oxalicum powder;
the paecilomyces lilacinus powder is obtained by fermenting paecilomyces lilacinus WZ004 with the preservation number of CGMCC No. 13761;
the penicillium oxalicum powder is obtained by fermenting penicillium oxalicum WZ006 with the preservation number of CGMCC No. 13763.
2. The compound microbial wettable powder for controlling nematodes according to claim 1, wherein the spore content of the paecilomyces lilacinus powder is not less than 120 hundred million/g, and the spore content of the penicillium oxalicum powder is not less than 120 hundred million/g.
3. The compound microbial wettable powder of claim 1, which further comprises an auxiliary agent; the auxiliary agent comprises kaolin, a diffusant NNO and sodium lignosulfonate.
4. The compound microbial wettable powder of claim 3, which is prepared from the following components in percentage by mass: 40% of paecilomyces lilacinus powder, 30% of penicillium oxalicum powder, 10% of kaolin, 10% of dispersing agent NNO and 10% of sodium lignosulphonate.
5. A preparation method of the compound microbial wettable powder of any one of claims 1 to 4, which is characterized by comprising the following steps:
step 1: respectively inoculating paecilomyces lilacinus WZ004 with the preservation number of CGMCC No.13761 and penicillium oxalicum WZ006 with the preservation number of CGMCC No.13763 on a PDA plate medium, culturing at 27 ℃ in the dark for 7 days, and taking the cultured plate as an original strain;
step 2: respectively inoculating the stock seeds cultured by Step1 on a PDA liquid culture medium, culturing at 27 ℃ in the dark at 121rpm for 4d, and taking the fermented liquid as a seed liquid;
step 3: adding water into testa Tritici, controlling humidity at 20%, and sterilizing at 121 deg.C for 30min to obtain fermentation culture medium;
step 4: adding the seed solution cultured in Step2 into the fermentation medium prepared in Step3, and standing and dark culturing at 27 deg.C for 14 d.
Step 5: respectively drying and crushing fermentation liquor prepared by Step4, and sieving the fermentation liquor with a 100-mesh sieve to obtain paecilomyces lilacinus powder with spore content of more than or equal to 120 hundred million/g and penicillium oxalicum powder with spore content of more than or equal to 120 hundred million/g;
step 6: mixing the paecilomyces lilacinus powder and the penicillium oxalicum powder prepared by Step5 with kaolin, a dispersing agent NNO and sodium lignosulphonate uniformly to obtain the paecilomyces lilacinus powder.
6. Use of the compound microbial wettable powder of any one of claims 1 to 4 for controlling nematodes.
7. The use of the compound microbial wettable powder of any one of claims 1 to 4 for controlling soil-borne diseases.
8. The use of the compound microbial wettable powder of any one of claims 1 to 4 in phosphate and potassium dissolution.
9. The use of a composite microbial wettable powder of any one of claims 1 to 4 in the promotion of plant growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210011877.5A CN114424775A (en) | 2017-05-04 | 2017-05-04 | Compound microorganism wettable powder for preventing and treating nematodes and preparation method and application thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210011877.5A CN114424775A (en) | 2017-05-04 | 2017-05-04 | Compound microorganism wettable powder for preventing and treating nematodes and preparation method and application thereof |
| CN201710306254.XA CN107027813A (en) | 2017-05-04 | 2017-05-04 | A kind of complex microorganism wettable powder for preventing and treating nematode and preparation method thereof |
Related Parent Applications (1)
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| CN102894012A (en) * | 2012-05-29 | 2013-01-30 | 中国林业科学研究院森林生态环境与保护研究所 | Paecilomyces lilacinus wettable powder, preparation method and application thereof |
| CN105523835A (en) * | 2015-12-24 | 2016-04-27 | 安徽省司尔特肥业股份有限公司 | Bioorganic fertilizer for adsorbing heavy metals and improving greenhouse soil, and preparation method thereof |
| CN105861336A (en) * | 2016-05-19 | 2016-08-17 | 中国农业科学院植物保护研究所 | Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof |
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| CN1252247C (en) * | 2003-02-24 | 2006-04-19 | 唐文华 | Oxalic acid penicillium, bacterial preparation thereof and its preparation method, and the application of the preparation in dephosphorization, prophylaxis and soil fertility enhancement |
| US20120021906A1 (en) * | 2009-03-23 | 2012-01-26 | John Clifford Sutton | Fungal inoculant compositions |
| CN106282033A (en) * | 2016-08-15 | 2017-01-04 | 郑毅男 | The one new penicillium of strain and metabolite thereof are pacified him and are intended acid A |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102894012A (en) * | 2012-05-29 | 2013-01-30 | 中国林业科学研究院森林生态环境与保护研究所 | Paecilomyces lilacinus wettable powder, preparation method and application thereof |
| CN105523835A (en) * | 2015-12-24 | 2016-04-27 | 安徽省司尔特肥业股份有限公司 | Bioorganic fertilizer for adsorbing heavy metals and improving greenhouse soil, and preparation method thereof |
| CN105861336A (en) * | 2016-05-19 | 2016-08-17 | 中国农业科学院植物保护研究所 | Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof |
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