CN114403442A - 一种芥菜提取物合生元及其制备方法 - Google Patents
一种芥菜提取物合生元及其制备方法 Download PDFInfo
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- CN114403442A CN114403442A CN202210086322.7A CN202210086322A CN114403442A CN 114403442 A CN114403442 A CN 114403442A CN 202210086322 A CN202210086322 A CN 202210086322A CN 114403442 A CN114403442 A CN 114403442A
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- bifidobacterium
- mustard
- probiotics
- synbiotics
- mustard extract
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Abstract
本发明公开了一种芥菜提取物合生元及其制备方法，涉及天然物质的提取以及微生物发酵领域；所述合生元包括益生元和益生菌，益生元以芥菜为主要原料，提取芥菜中多糖、萝卜硫素、硫苷、维生素等成分，通过与益生菌(长双歧杆菌婴儿亚种ATCC 15697、双歧杆菌M‑63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB 12、动物双歧杆菌BB 536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌)混合发酵等操作从而得到一种合生元产品。该制备方法制得的合生元通过益生菌与芥菜天然组分的交互作用实现调节肠道消化吸收功能、提神醒脑、缓解疲劳等效果，具有重要的应用价值。
Description
技术领域
本发明涉及合生元技术领域，特别是涉及调节肠道消化吸收功能，维持肠道健康及提高机体免疫力的芥菜提取物合生元及其制备方法。
背景技术
随着经济的飞速发展，人们越来越重视保健，而肠道菌群失调引发的肠道疾病在我们身边已经很常见，在此背景下，发明一种具有调节肠道消化吸收功能、维持肠道健康及提高机体免疫力的合生元具有现实意义。
芥菜含有维生素A、维生素C、维生素E和大量的抗坏血酸等，抗坏血酸参与机体重要的氧化还原过程，能增加大脑中氧含量，有提神醒脑，解除疲劳的作用。此外还有多酚，葡萄糖醛，含硫化合物和类胡萝卜素(β-胡萝卜素，叶黄素，玉米黄质)等物质，研究发现其对人体健康能产生积极影响。
肠道菌群失调可能会引发肠道疾病，我们通过加入益生菌，调节肠道内菌群平衡，促进营养吸收，保持肠道健康。本专利涉及的长双歧杆菌婴儿亚种ATCC15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB12、动物双歧杆菌BB536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌均为肠道益生菌，对机体有益。
我们研究发现益生菌可以发酵芥菜提取物产生有利于人体健康的物质，如多种维生素、多酚、萝卜硫素等。我们测定了有益物质的含量，同时进行了体外发酵实验和相关功能性的研究，探讨肠道菌群发酵芥菜提取物的方法及价值，为未来的合生元产品制备及工厂化生产提供参考。
发明内容
提供了一种芥菜提取物合生元及其制备方法。
发明目的：本发明所要解决的技术问题是针对现有技术的不足提高芥菜利用率及价值，提供一种芥菜提取物合生元及其制备方法。本发明另一个目的是提供芥菜提取物合生元在制备肠道微生态调节、改善肠道屏障及消化能力和增强免疫力的保健品中的应用。
技术方案：为了实现以上目的，本发明采取的技术方案为：一种芥菜提取物合生元，它是由多种菌发酵芥菜提取物制备得到。
一种合生元的制备方法，包括以下步骤：
(1)益生元的制备
称取芥菜，洗净后去皮切块或切丝，粉碎后置于无菌水中漂烫，用10倍体积的95％乙醇浸提，超声波辅助提取，过滤提取液，旋转蒸发浓缩，制成芥菜提取物溶液，4℃保存备用。
(2)益生菌的培养
配制MRSC培养基，其中含0-1％L-CysteineHCl，初始pH值控制在6.4～6.6之间，并经高压灭菌、冷却、静置保存；
取等量培养基分别接种长双歧杆菌婴儿亚种ATCC15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB12、动物双歧杆菌BB536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌，于37℃24h活化并增殖。
(3)合生元的制备
将增殖后的菌按体积比1％～5％的接种量接种于益生元中，静置于厌氧培养箱中发酵，冷冻干燥。
经上述操作后即制得合生元冻干粉。
作为优选方案，所述芥菜在提取前进行切块，并无菌操作。
作为优选方案，以上所述的合生元的制备方法，MRSC培养基为MRS培养基加入0.05％L-CysteineHCl，初始pH值为6.5±0.1。
作为优选方案，发酵条件为厌氧，37℃，48h。
作为优选方案，以上所述的合生元的制备方法，微孔滤膜孔径为0.22μm。
作为优选方案，所制得合生元为粉状物，真空密封包装进行保存。
有益效果：
1.本发明首先创造性的提取得到芥菜提取物，包括多种营养成分，并添加优选的多种益生菌，芥菜提取物与多种益生菌共同发酵生产制备合生元，不仅可以有效利用东北芥菜资源，还可以得到优良的合生元制剂，该发明对东北特色资源的综合高效利用具有积极的推动作用。
2.实验结果表明，本发明提供的芥菜提取物作为发酵来源，能很好的促进益生菌的生长。表明本发明提供的芥菜提取物是多种益生菌繁殖的优良营养源。
3.本发明制备得到的芥菜提取物合生元可提高改善肠道屏障及消化能力和增强免疫力，具有重要的应用价值。
附图说明
图1为芥菜提取物合生元产品制备工艺流程图。
具体实施方式
以下结合具体实施例对本发明的芥菜提取物合生元及其制备方法作进一步详细的说明。
实施例1，制备一种以芥菜块为原料的合生元
称取芥菜，洗净后去皮切块，切块大小为1cm×1cm，。将芥菜块置于90-100℃无菌蒸馏水中漂烫10-15s，使用10倍体积的95％乙醇作为溶剂，浸提24h，超声波辅助提取2h，过滤提取液，旋转蒸发(40～50℃)浓缩，制成浓度为1-20g/L的芥菜提取物，4℃保存备用。
配制无菌MRSC培养基，为MRS培养基加入0.05％L-CysteineHCl。分别接种长双歧杆菌婴儿亚种ATCC15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB12、动物双歧杆菌BB536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌，37℃24h传3代。分别接种于含1-20g/L芥菜提取物的MRSC培养液中培养24h，连续传3代，获得适应芥菜提取物的益生菌，保存备用。
以2％-5％接种量接种适应芥菜提取物的益生菌于1-20g/L芥菜提取物中，置于厌氧培养箱中发酵，发酵温度为37-42.5℃，发酵时间为24-48h，冷冻干燥，即得合生元。该合生元为粉状物，将其铝箔真空包装保存。
实施例2，制备一种以芥菜丝为原料的合生元
称取芥菜，洗净后去皮切丝，切丝长宽为2cm×0.5cm。将芥菜丝置于90-100℃无菌蒸馏水中漂烫10-15s，使用10倍体积的95％乙醇作为溶剂，浸提24h，超声波辅助提取2.0h，过滤提取液，旋转蒸发(40～50℃)浓缩，制成浓度为1-20g/L的芥菜提取物，4℃保存备用。
配制无菌MRSC培养基，为MRS培养基加入0.05％L-CysteineHCl。分别接种长双歧杆菌婴儿亚种ATCC15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB12、动物双歧杆菌BB536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌，37℃24h传3代。分别接种于含1-20g/L芥菜提取物的MRSC培养液中培养24h，连续传3代，获得适应芥菜提取物的益生菌，保存备用。
以2％-5％接种量接种适应芥菜提取物的益生菌于1-20g/L芥菜提取物中，置于厌氧培养箱中发酵，发酵温度为37-42.5℃，发酵时间为24-48h，冷冻干燥，即得合生元。该合生元为粉状物，将其铝箔真空包装保存。
对照实施例
将实施例1、实施例2培养的益生菌以体积比2％的接种量接种于用孔径为0.22μm微孔滤膜过滤的无菌MRSC培养液中，于37℃培养24h，进行冷冻干燥，即得对照物。
实施例中各性能指标的测试标准或方法：
(1)AlicanBlue法测定多糖浓度
(2)紫外分光光度计法测总酚类化合物含量
(3)通过乙醇溶液变色来评估芥菜提取物的自由基清除活性DPPH自由基的合成。
(4)通过金属催化氧化反应吸光度变化测定金属螯合活性。
(5)用β-胡萝卜素漂白试验测定抗氧化活性。
(6)超氧化物自由基清除活性。
(7)还原力的测定。
(8)萝卜硫素的丙酮提取及HPLC分析测定。
(9)高效液相色谱法测定维生素含量。
(10)平板菌落计数
从上述实施例可知，在本发明中,芥菜提取物能促进益生菌的生长，本发明芥菜提取物合生元可以有效调节肠道消化吸收功能，维持肠道健康及提高机体免疫力，且通过益生菌与芥菜天然组分的配合使用来达到提神醒脑、缓解疲劳、抗氧化抗衰老等效果，具有重要的应用价值。应当理解的是,对本领域技术人员来说，可以根据上述说明加以改进或变换，而所有这些改进和变换都应属于本发明所附权利要求的保护范围。
Claims (10)
1.一种芥菜提取物合生元，包括益生元和益生菌，其特征在于：
所述益生元为芥菜提取物，包括但不限于多糖、维生素A、维生素C、维生素E、抗坏血酸、多酚、含硫化合物萝卜硫素和类胡萝卜素(β-胡萝卜素，叶黄素，玉米黄质)等物质；所述益生菌包括长双歧杆菌婴儿亚种ATCC 15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB 12、动物双歧杆菌BB 536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌；利用益生菌发酵益生元得到芥菜提取物合生元。
2.根据权利要求1所述的益生元，其特征在于：
益生元的制备方法为：称取芥菜，洗净后去皮切块或切丝，粉碎后置于无菌水中漂烫10-30秒，使用10倍体积的95％乙醇作为溶剂，浸提24h，超声波辅助提取2.0h，过滤提取液，旋转蒸发(40～50℃)浓缩，制成浓度为1-20g/L的芥菜提取物，4℃保存备用。
3.根据权利要求1所述的合生元，其特征在于：
所述芥菜块大小为1cm×1cm，切丝长宽为2cm×0.5cm，粉碎至糊状，漂烫温度为90-100℃，漂烫时间为6-20s；更优选地，所述提取的条件为95-100℃，10-15s。所得芥菜提取物为1-20g/L的液体。
4.根据权利要求1所述的合生元，其特征在于：
合生元中所述益生菌液的制备过程如下：配制无菌MRS/MRSC培养基，分别接种长双歧杆菌婴儿亚种ATCC 15697、双歧杆菌M-63、罗氏乳杆菌、植物乳杆菌、副干酪乳杆菌、鼠李糖乳杆菌、动物双歧杆菌BB 12、动物双歧杆菌BB 536、青春双歧杆菌、两歧双歧杆菌、短双歧杆菌、乳双歧杆菌，37℃24h活化2次。
5.根据权利要求1所述的合生元，其特征在于：
所述益生菌的接种量为1％-10％；然后分别接种到含芥菜提取物的MRS/MRSC培养液中培养24h，连续传3代，获得适合生长于芥菜提取物的益生菌，保存备用。优选地，接种量为1％-5％，所述接种适应芥菜提取物的活菌数在107-8cfu/mL以上。
6.根据权利要求1所述的合生元，其特征在于：
益生菌离心洗涤后，以1％-5％接种量接种适应芥菜提取物的益生菌于1-20g/L芥菜提取物中，置于厌氧培养箱中发酵，冷冻干燥，即得合生元。微生物发酵条件为35-45℃，24-72h；优选地，所述发酵条件为37-42.5℃，厌氧发酵24-48h。
7.根据权利要求1所述合生元，其特征在于：
总酚类化合物的含量介于16.50-41.40mg/g(没食子酸浓度)。自由基清除活性随浓度提高而增强，在750和1000μg/mL浓度下，1,1-二苯基-2-三硝基苯肼(DPPH)活性几乎达到最高水平，活性为70.69％。亚铁离子螯合力活性为80％-90％。芥菜提取物的β-胡萝卜素漂白活性在100-1000μg/mL之间。芥菜提取物超氧化物自由基清除活性为40-45％。萝卜硫素含量为12-20μg/g。维生素A0-17μg/g，维生素C 0.37-0.74mg/g，维生素E 0.03-0.06mg/g。
8.根据权利要求1所述合生元，其特征在于：
发酵后将其冻干为粉状物，最后铝箔真空包装保存。
9.如权利要求1-8任一项所述合生元在医疗保健中的应用。
10.根据权利要求9所述的应用，所述医疗保健用于有效调节肠道消化吸收功能，维持肠道健康及提高机体免疫力。所述合生元通过益生菌与芥菜天然组分的配合使用可达到提神醒脑、缓解疲劳等效果，同时具有抗氧化抗衰老等功效。
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| US20080292751A1 (en) * | 2005-05-27 | 2008-11-27 | Kabushiki Kaisha Yakult Honsha | Lactic Acid Bacteria Fermented Substance and Fermented Milk Food Product Containing the Same |
| KR20160080431A (ko) * | 2014-12-29 | 2016-07-08 | 더나눔협동조합 | 채소류 복합 발효 추출물, 이의 제조방법 및 이를 포함하는 항산화용 식품 조성물 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116904346A (zh) * | 2023-06-06 | 2023-10-20 | 石河子大学 | 动物双歧杆菌乳亚种hn-3在沙枣果汁发酵中的应用 |
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