CN114392780A

CN114392780A - Collection device and detection device for collecting liquid samples

Info

Publication number: CN114392780A
Application number: CN202111398025.8A
Authority: CN
Inventors: 王中平
Original assignee: Acon Biotech Hangzhou Co Ltd
Current assignee: Acon Biotech Hangzhou Co Ltd
Priority date: 2021-11-19
Filing date: 2021-11-19
Publication date: 2022-04-26
Also published as: WO2023088405A1

Abstract

The invention provides a collecting device and a detecting device for collecting liquid samples, which comprise a liquid sample collecting bottle and a sampler, wherein the inner wall of the liquid sample collecting bottle is provided with a guide groove which inclines from top to bottom and spirally descends, and the lower end of the rod part of the sampler is provided with a guide rail. After the lower end of the rod part of the sampler is inserted into the liquid sample collecting bottle, the guide rail at the lower end of the rod part is matched with the guide groove on the inner wall of the bottle body to guide the lower end of the rod part of the sampler to spirally descend in the liquid sample collecting bottle, so that the liquid drawing part of the sampler is wrung. Compared with the prior art, the liquid sample squeezing device has higher liquid sample squeezing rate, so that the minimum amount of the liquid sample required by detection is more easily met, and the detection success rate and the detection result accuracy are further improved. The invention can be used for collecting and detecting biological samples such as infectious pathogens.

Description

Collection device and detection device for collecting liquid samples

Technical Field

The invention belongs to the technical field of medical instruments, further belongs to the field of medical examination, and particularly relates to a collecting device and a detection device for collecting a liquid sample.

Background

There are a variety of clinical lateral flow immunoassay devices that can be used to detect the presence of an analyte in a sample, such as hormones, sugars, adulterants, drugs of abuse (including injection or drug abuse), and the like. The detection of a certain component or components in a liquid sample is typically performed after the liquid sample has been collected and added to a lateral flow immunoassay device.

Wherein the liquid sample may be any volume of liquid that is tested, examined, or studied. The liquid sample may be derived from any liquid, for example a bodily fluid such as saliva or blood or urine or other liquid to be tested, such as a water sample from a source of contamination, a liquid sample from a beverage, etc.

Liquid samples are typically collected and absorbed using a sampling wand having a collection element formed of an absorbent material such as a pad or sponge attached to one end of a plunger. The collecting element is such that a sufficient volume of sample liquid can be absorbed from the liquid source, the presence of the sample liquid in the collecting element causing the interior of the collecting element to expand. The collection element typically retains the sample until the collection element is manipulated to release the sample. In the detection, the prior art generally adopts a squeezing mode to squeeze the liquid sample out of the collecting element of the sampling rod, and then the liquid sample is added to a detection device (such as a lateral flow immunoassay device) for detection; or in some detection, the squeezed liquid sample is firstly mixed with a buffer solution for pretreatment such as cracking and the like, and then is added to a detection device for detection.

In the prior art, a collection bottle is used to collect a liquid sample on a collection element, i.e., the collection element end of a sampling rod is inserted into the collection bottle, and the collection element is pressed against the sidewall or bottom of the collection bottle body to cause the liquid sample to flow out of the collection bottle for sample collection. The existing collection bottle and the existing sampling rod have the problems that the liquid sample on the collection element of the sampling rod cannot be sufficiently extruded, namely, more liquid sample still remains on the collection element after extrusion, so that only less liquid sample is used for subsequent detection, and thus the amount of the liquid sample used for detection is easily insufficient, and detection failure or inaccurate detection result is easily caused (especially when the liquid sample is saliva, the amount of saliva released by a human or an animal in a short time is generally very limited, and the amount of the liquid sample is easily insufficient). In addition, when buffer solution is in the collection bottle, the collection element can absorb and take away part of the buffer solution or the mixed solution of the liquid sample and the buffer solution, so that the accuracy of the detection result is affected.

Disclosure of Invention

One of the objects of the present invention is: in response to the deficiencies of the prior art, a liquid sample collection bottle is provided for collecting a liquid sample from a human or animal.

The above object of the present invention is achieved by the following technical solutions:

a liquid sample collection bottle comprising a body having an opening, the body comprising an inner wall and an outer wall, the inner wall comprising a cylindrical portion, wherein: the cylindrical part of the inner wall of the bottle body is provided with a guide groove extending downwards from the opening, and the guide groove comprises a part which is inclined from top to bottom and spirally descends.

As a further improvement of the invention, the cross section of the guide groove is rectangular, triangular, trapezoidal or circular arc, and the part of the guide rail with larger cross section dimension is positioned on the inner wall of the bottle body.

As a further improvement of the invention, after the inner wall of the bottle body is unfolded, the guide groove comprises a vertical part and an inclined part from top to bottom.

As a further improvement of the invention, after the inner wall of the bottle body is unfolded, the inclined part of the guide groove is a straight line or a smooth curve.

As a further improvement of the invention, the slope of the smooth curve decreases from top to bottom.

As a further improvement of the invention, the guide groove further comprises a horizontal surrounding part communicated with the bottom of the inclined part of the guide groove.

As a further improvement of the invention, the bottle also comprises a backing plate placed in the bottle body, and the backing plate is provided with a through hole for facilitating the liquid sample to pass through.

As a further improvement of the invention, the upper surface of the backing plate is provided with a projection or a recess.

As a further improvement of the invention, the guide rail spirally descends one sixth to one sixth of the inner wall of the bottle body.

The second purpose of the invention is that: in view of the shortcomings of the prior art, a collection device for collecting a liquid sample is provided for collecting a liquid sample of a human or an animal.

The above object of the present invention is achieved by the following technical solutions: a collecting device for collecting a liquid sample, comprising a liquid sample collecting bottle and a sampler, wherein the liquid sample collecting bottle comprises a bottle body with an opening, the bottle body comprises an inner wall and an outer wall, the inner wall comprises a cylindrical part, the sampler comprises a rod part and a liquid drawing part, the liquid drawing part is connected to the lower end of the rod part, wherein: the cylindrical part of the inner wall of the bottle body is provided with a guide groove which extends downwards from the opening, the guide groove comprises a part which inclines from top to bottom and spirally descends, the lower end of the rod part is provided with a guide rail, after the lower end of the rod part of the sampler is inserted into the liquid sample collecting bottle, the guide rail at the lower end of the rod part is matched with the guide groove of the inner wall of the bottle body, and the lower end of the rod part of the sampler is guided to spirally descend in the liquid sample collecting bottle.

As a further improvement of the invention, the cross section of the guide groove of the inner wall of the bottle body is rectangular, triangular, trapezoidal or circular arc, and the part of the guide rail with larger cross section dimension is positioned on the inner wall of the bottle body.

As a further improvement of the invention, after the inner wall of the bottle body of the liquid sample collecting bottle is unfolded, the guide groove comprises a vertical part and an inclined part from top to bottom.

As a further improvement of the invention, after the inner wall of the bottle body of the liquid sample collection bottle is unfolded, the inclined part of the guide groove is a straight line or a smooth curve.

As a further improvement of the invention, the liquid sampler further comprises a backing plate placed in the liquid sample collecting bottle, wherein the backing plate is provided with a through hole for the liquid sample to pass through, and further the liquid sucking part of the sampler is squeezed in the process that the lower end of the rod part of the sampler is spirally descended in the liquid sample collecting bottle.

As a further improvement of the invention, the base plate and the liquid drawing part of the sampler are provided with mutually matched bulges and depressions.

As a further improvement of the invention, the bottom of the liquid sucking part is hardened to have higher hardness relative to other parts of the liquid sucking part, thereby being more beneficial to reducing the slippage between the bottom of the liquid sucking part and the upper surface of the base plate.

As a further improvement of the invention, the guide groove spirally descends one sixth to one week of the inner wall of the bottle body of the liquid sample collection bottle.

As a further improvement of the invention, the matched guide rail and the guide groove are in one pair, or two pairs, three pairs, four pairs, five pairs or six pairs which are uniformly distributed.

The third purpose of the invention is: in view of the deficiencies of the prior art, a method of collecting a liquid sample is provided for collecting a liquid sample of a human or animal.

a method of collecting a liquid sample, providing a collecting device for collecting a liquid sample, the collecting device for collecting a liquid sample comprising a sampler and a liquid sample collecting member, an inclined and spirally descending guide mechanism being provided between the sampler and the liquid sample collecting member for guiding the sampler to spirally descend within the liquid sample collecting member, the method comprising the steps of:

A. making the liquid drawing part of the sampler draw a predetermined amount of liquid samples;

B. inserting the liquid drawing part of the sampler into the liquid sample collecting component to ensure that the inclined guide rail of the sampler corresponds to the inclined and spirally descending guide groove on the inner wall of the liquid sample collecting component;

C. pushing the sampler to move downwards, and leading the sampler to spirally descend in the liquid sample collecting part under the guiding of the mutual matching of the guide groove and the guide rail, so as to screw and squeeze the liquid sucking part of the sampler until the liquid sucking part is fully screwed and squeezed;

D. the sampler is reversely coiled and raised, and the sampler is taken out and the liquid sample collecting part is sealed.

As a further improvement, the collecting device for collecting the liquid sample is disclosed in the second invention.

The fourth purpose of the invention is: in response to the deficiencies of the prior art, a testing device is provided for collecting a fluid sample from a human or animal.

a detection device comprises a collection device for collecting liquid samples and a detection plate, wherein the collection device for collecting the liquid samples comprises a liquid sample collection part and a sampler, the liquid sample collection part comprises a cylindrical inner wall, the sampler comprises a rod part and a liquid drawing part, and the liquid drawing part is connected to the lower end of the rod part, wherein: the cylindrical part of the inner wall of the bottle body is provided with a guide groove extending downwards from the opening, the guide groove comprises a part which inclines from top to bottom and spirally descends, the lower end of the rod part is provided with a guide rail, after the lower end of the rod part of the sampler is inserted into the liquid sample collecting bottle, the guide rail at the lower end of the rod part is matched with the guide groove of the inner wall of the bottle body, the lower end of the rod part of the sampler is guided to spirally descend in the liquid sample collecting bottle, and then the liquid drawing part of the sampler is squeezed, the detection plate comprises a detection part, and the detection part is used for giving out detection result information.

As a further development of the invention, the liquid sample collection member and the detection panel or a part of the detection panel are separate and independent elements from each other.

As a further development of the invention, the liquid sample collection member is mounted on the detection plate or a component of the detection plate.

Advantageous effects

The inner wall of the liquid sample collecting bottle is provided with a guide groove extending downwards from the opening, the guide groove comprises a part which inclines from top to bottom and spirally descends, the lower end of the rod part of the sampler is provided with a guide rail, the liquid drawing part of the sampler is inserted into the sampling bottle after drawing enough liquid samples, and the lower end of the rod part of the sampler is guided to drive the liquid drawing part to spirally descend in the sampling bottle under the mutual matching of the guide rail and the guide groove, so that the liquid drawing part is twisted and squeezed, and the liquid samples drawn by the liquid drawing part are fully squeezed out. Compared with the prior art, the liquid sample squeezing device has higher liquid sample squeezing rate, and can squeeze more liquid samples for detection under the condition that the same liquid sample is drawn by the liquid drawing part, so that the minimum amount of the liquid sample required by detection is more easily met, and the success rate of the detection and the accuracy of the detection result are improved.

Drawings

FIG. 1 is a schematic plan view of a liquid sample collection vial of the present invention.

Fig. 2 is a schematic sectional view taken along line a-a of fig. 1.

FIG. 3 is a schematic plan view of a pad within a liquid sample collection bottle of the present invention.

Fig. 4 is a top view of fig. 1.

Fig. 5 is a schematic plan view of a collection device of the present invention for collecting a liquid sample (without a sampler inserted into the liquid sample collection vial).

Fig. 6 is a partial cross-sectional view taken along line a-a of fig. 5.

Fig. 7 is a view similar to fig. 5, but with the sampler inserted in the initial position in the liquid sample collection bottle (the scooping portion not yet squeezed).

Fig. 8 is a schematic sectional view taken along line a-a of fig. 7.

Fig. 9 is a partially enlarged schematic view of a portion "a" in the direction C-C of fig. 8.

Fig. 10-12 are schematic views of the modified design of fig. 9.

FIG. 13 is a partially expanded plan view of the cylindrical portion of the inner wall of the liquid sample collection vial of the present invention.

Fig. 14 is a schematic view of a modified design of fig. 13.

Fig. 15 is a view similar to fig. 7, but with the sampler inserted in the final position in the liquid sample collection vial (the swab fully squeezed).

Fig. 16 is a schematic sectional view taken along line a-a of fig. 15.

Fig. 17 is a schematic view of a modified design of fig. 2.

FIG. 18 is a view similar to FIG. 7, but showing the configuration of the fluid specimen collection bottle shown in FIG. 17.

Fig. 19 is an enlarged schematic view of a portion "B" in fig. 18.

Fig. 20 is a modified design diagram of fig. 8, showing another modified design of the present invention.

Fig. 21 is a partially enlarged view of fig. 20.

Fig. 22 is a modified design diagram of fig. 21.

FIGS. 23-28 illustrate six embodiments of the sampling wand of the present invention.

Fig. 29 and 30 are modified design diagrams of fig. 13.

Fig. 31 and 32 are modified design diagrams of fig. 14.

FIG. 33 is a schematic view of the detecting device of the present invention in use.

Fig. 34 is a schematic view showing the detection result of the detection plate shown in fig. 33.

FIG. 35 is a schematic view of another embodiment of the detecting device of the present invention.

Fig. 36 is a schematic view showing the detection result of the detection plate shown in fig. 35.

Detailed Description

The invention is described in further detail with reference to the figures and specific embodiments.

The above-described embodiments are intended to illustrate the present invention, but not to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit of the present invention and the scope of the claims fall within the scope of the present invention.

Interpretation of terms-patent specification (including claims and abstract) of this invention describes the terms used, and it is to be understood that this specification is to be interpreted accordingly, and not by way of limitation, as is the ordinary meaning in the art.

Cylinder: the cylinder of the invention is a cylinder with a circular or approximately circular cross section and equal or approximately equal area of each cross section; the cylinder of the invention also includes a truncated cone, i.e. the longitudinal section through its axis is isosceles or approximately isosceles, but preferably the angle between the sloping side of the trapezoid and its height does not exceed fifteen degrees. In summary, the cylindrical body for achieving the advantageous effects of the present invention is included in the scope of the cylindrical body of the present invention, as long as the person skilled in the art considers that the object of the present invention can be achieved and the technical problems to be solved by the present invention can be solved.

A cylindrical portion: the shape or configuration is that portion of the cylinder of the present invention.

Liquid sample collection bottle: also known as a liquid sample collection member, refers to a bottle-like object, a cup-like object, a cylindrical object, and any shape object or portion of an object having a cylindrical inner wall for collecting a liquid sample.

Spiral descending: also called spiral descending, namely, the inner wall of the cylindrical part gradually descends in a spiral form from high to low to form a track of a three-dimensional smooth curve. The spiral may be from a fraction of a week to a week or weeks.

Rectangle, triangle, trapezoid: the method not only comprises rectangles, triangles and trapezoids in mathematical concepts, but also comprises approximate rectangles, triangles and trapezoids.

Forward: the direction opposite to the reverse direction is referred to, but the forward direction does not specifically mean the clockwise direction, but means one of the clockwise direction and the counterclockwise direction. When the forward direction refers to the clockwise direction, the reverse direction refers to the counterclockwise direction; conversely, when forward refers to counterclockwise, reverse refers to clockwise.

Twisting and extruding: twisting while squeezing, i.e. the two ends of the object are brought closer together and at the same time are brought into a rotating movement in opposite directions, creating an effect similar to wringing out a towel.

The liquid-sucking part is sufficiently wrung: the method is characterized in that under the action of normal manpower, the liquid drawing part is squeezed to achieve the effect of basically discharging liquid in the liquid drawing part (the liquid discharging rate is obviously higher than that of the prior art).

Referring to fig. 1 and 2, the present invention provides a liquid sample collection bottle 10, which is used for collecting liquid samples such as saliva, blood, urine, sweat, etc. of a human or an animal, and after processing, detecting the content of one or some substances in the liquid samples by using a biological detection method (including an immunological detection method and a non-immunological detection method), so as to obtain physiological parameter indexes of the detected human or animal. Taking the detection of the novel coronavirus pneumonia as an example, scientists have developed products and detection methods for detecting saliva (including processed saliva) of human or animals as samples to determine whether the human or animal providing the saliva samples is infected with the novel coronavirus pneumonia. One of the important steps of the detection method is how to collect saliva of a human body or an animal to be detected. The invention aims to provide a novel liquid sample collecting bottle for collecting human or animal saliva, a collecting device for collecting liquid samples and a method for collecting the liquid samples by using the collecting device for collecting the liquid samples. The invention can be used for detecting the novel coronavirus pneumonia.

The invention comprises the following steps: liquid sample collection bottle

Referring to fig. 1 and 2, a liquid sample collection bottle 10 according to the present invention includes a bottle body 12 having an opening 11, the bottle body 12 includes an inner wall 14 and an outer wall 16, the inner wall 14 includes a cylindrical portion 18 (i.e., at least a portion of the inner wall 14 is cylindrical), and the cylindrical portion 18 is provided with a guide groove 20 that is inclined and spirally descending from the opening 11. The guide groove 20 which inclines from top to bottom and spirally descends at least comprises the following structures: A. the whole section from the starting point to the end point is a spiral descending part 20b (as shown in fig. 29 and 31); B. a vertically descending part 20a vertically extending downward from the opening 11 of the bottle body by a suitable distance and a spirally descending part 20b communicating with the vertically descending part 20a (as shown in FIGS. 2, 6, 13, 14); C. a vertically descending part 20a vertically extending downward from the opening 11 of the bottle body by a suitable distance, a spirally descending part 20b communicating with the vertically descending part 20a, and a horizontally surrounding part 20c communicating with the spirally descending part 20b (as shown in fig. 17); and D, a spiral descending portion 20b spirally descending from the opening 11 of the bottle body, and a horizontal surrounding portion 20c communicating with the spiral descending portion 20b (see FIGS. 30 and 32). The channel 20 is a groove recessed an appropriate distance into the inner wall 14 and attached to the inner wall 14 in a spiral-shaped disc. Referring to fig. 9-12, the cross-section of the channel 20 is rectangular (including square), triangular, trapezoidal, or circular (including partial circular or partial oval), and the larger cross-sectional dimension of the channel 20 (shown by the dashed lines in fig. 9-12) is located on the inner wall 14 of the bottle 12.

In one embodiment, after the cylindrical portion 18 of the inner wall 14 of the bottle body 12 is unfolded, the spiral descending portion 20b of the inner wall 14 is a straight line inclined from top to bottom (as shown in fig. 13, 29 and 31), and the included angle "a" between the straight line and the horizontal plane is greater than or equal to thirty degrees and less than or equal to seventy-five degrees, and the preferred included angle "a" is sixty degrees or forty-five degrees. In another embodiment, after the inner wall 14 of the bottle body 12 is unfolded, the spiral descending portion 20b of the inner wall 14 is a smooth curve inclined from top to bottom (as shown in fig. 14, 31 and 32), and the slope of the smooth curve decreases from top to bottom (i.e. the included angle between the tangent line of the smooth curve and the horizontal plane gradually decreases). Preferably, this embodiment includes an angle "C" of the tangent to the top of channel 20 to the horizontal of seventy-five degrees and an angle "B" of the tangent to the bottom of channel 20 to the horizontal of thirty degrees.

As shown in fig. 2, the fluid specimen collection bottle 10 also includes a pad 22 disposed within the body 12. The backing plate 22 is provided with a plurality of through holes 24 for facilitating the flow of the liquid sample therethrough. The backing plate 22 is in the form of a disc having a thickness of about 2.0-5.0 mm and is typically made of a rigid plastic. In the embodiment shown in fig. 1 and 2, the body 12 includes upper and lower portions, the upper portion being of a substantially larger size than the lower portion, a shoulder 26 being formed at the junction of the two portions. The backing plate 22 is placed over the shoulder 26 and supported by the shoulder 26. In one embodiment, the pad 22 may be omitted when the area of the shoulder 26 is twice or more the cross-sectional area of the inner diameter of the lower portion of the bottle, i.e., the pad 22 is not required. Fig. 3 is a plan view of the mat 22, and fig. 4 is a plan view of the mat 22 after it has been placed within the bottle 12. In one embodiment, the upper surface of the backing plate 22 is provided with a recess (the recess may also be a through hole 24, as shown in fig. 21) or a protrusion (as shown in fig. 22). The channels 20 are threaded down the interior wall 18 of the bottle at least one sixth of a revolution, but preferably no more than one revolution (not necessarily no more than one revolution, and in some cases, several revolutions). One channel 20 may be provided in the inner wall 18 of the cylindrical portion, or two, three, four, five and six channels 20 may be provided uniformly, but the number of channels 20 should not limit the scope of the present invention.

Invention two, collecting device for collecting liquid sample

Referring to fig. 5-8, the present invention further provides a collecting device 100 for collecting a liquid sample, wherein the collecting device 100 for collecting a liquid sample comprises a liquid sample collecting bottle 10 according to the first invention (which is incorporated in the second invention for avoiding repetition) and a sampler 50. Sampler 50 includes a stem portion 52 and a dip portion 56 connected to a lower end 54 of the stem portion. The liquid suction portion 56 is made of a material excellent in both water absorbing performance and water discharging performance, such as sponge, absorbent cotton, gauze, specialty fibers, and the like. The stem 52 is made of a hard material, and the lower end 54 of the stem is shaped like a pie, and has a raised guide 58 formed on its outer periphery, the guide 58 being shaped and dimensioned to correspond to the channel 20 in the interior wall 14 of the bottle (the guide 58 is inclined in a direction corresponding to the channel 20 as shown in fig. 5 and 6), so that the guide 58 can slide freely in the channel 20 and cooperate with each other to guide the lower end 54 of the stem to spirally descend. When the lower end 54 of the shaft portion of the sampler 50 is inserted into the liquid sample collection bottle 10, the guide rails 58 of the lower end 54 of the shaft portion are engaged with the guide grooves 20 on the inner wall 14 of the bottle body to guide the lower end 54 of the shaft portion of the sampler 50 to spirally descend in the liquid sample collection bottle. When the bottom of the liquid-drawing portion 56 presses the upper surface of the pad 22, the surface friction between the two reduces the relative sliding of the bottom of the liquid-drawing portion 56 with respect to the pad 22, but the guide groove 20 guides the rod lower end 54 to drive the upper portion of the liquid-drawing portion 56 to continue to twist and descend, so that the rod lower end 54 twists and twists the liquid-drawing portion 56 while pressing downward, thereby squeezing the liquid-drawing portion 56 (similar to squeezing a towel), and further discharging the liquid sample from the liquid-drawing portion 56 into the liquid sample collection bottle 10 more completely, and mixing with the lysate 60 (shown in fig. 8) in the collection bottle to form a mixture 62 (shown in fig. 16) of the lysate and the liquid sample.

As described in the first invention, after the cylindrical portion 18 of the inner wall 14 of the bottle body 12 is unfolded, the guiding groove 20 of the inner wall 14 is at least partially a straight line or a smooth curve 20b inclined from top to bottom. The smoothly curved channel facilitates the channel 20 and the guide rail 58 to guide the lower end 54 of the rod portion to increase the descending speed at the beginning of the descending stage, so as to generate stronger downward squeezing on the liquid drawing portion, and the channel 20 and the guide rail 58 to guide the lower end 54 of the rod portion to generate stronger torsion near the end stage of descending, so as to generate better squeezing effect on the liquid drawing portion 56, so as to squeeze more liquid samples from the liquid drawing portion 56.

As shown in fig. 20-22, in order to reduce the relative sliding between the bottom of liquid-drawing portion 56 and the upper surface of pad 22, there are protrusions 28 and recesses 30 on the upper surface of pad 22 and the bottom of liquid-drawing portion 56 of the sampler, which cooperate with each other to reduce the relative sliding between the bottom of liquid-drawing portion 56 and the upper surface of pad 22, and to enhance the squeezing effect of liquid-drawing portion 56. The depression 30 (the depression may be the through hole 24) may be provided on the upper surface of the backing plate 22 and the projection 28 may be provided on the bottom of the liquid-drawing portion 56 of the sampler (as shown in fig. 21), or the projection 28 may be provided on the upper surface of the backing plate 22 and the depression 30 may be provided on the bottom of the liquid-drawing portion 56 of the sampler (as shown in fig. 22). Preferably, the bottom of the liquid-drawing portion 56 is hardened to have higher hardness than other portions of the liquid-drawing portion, so as to be more beneficial to reducing the slippage between the bottom of the liquid-drawing portion 56 and the upper surface of the pad 22, improve the squeezing effect of the liquid-drawing portion 56, and further improve the liquid sample discharging rate of the liquid-drawing portion 56.

As shown in fig. 23, there is one guide rail 58 on the lower end 54 of the shaft of the sampler 50, and in this case, there is a pair of the guide groove 20 and the guide rail 58 which are engaged with each other. As shown in fig. 24-28, in order to improve the guiding smoothness, the guide rails 58 on the lower end 54 of the rod portion of the sampler 50 may be two, three, four, five, or six, which are uniformly distributed, and at this time, the guide grooves 20 and the guide rails 58 which are mutually matched are two pairs, three pairs, four pairs, five pairs, or six pairs, respectively, so that the squeezing effect on the liquid-drawing portion 56 can be improved, and the efficiency of squeezing the liquid sample from the liquid-drawing portion 56 can be further improved.

As shown in fig. 17-19, the distance "L" between the bottom of the horizontal surround 20c of the channel and the upper surface of the backing plate 22 is greater than or equal to 2.5 mm and less than or equal to 35.0 mm, which enables the rail 58 of the lower end 54 of the shaft of the sampler 50 to remain snapped onto the horizontal surround 20c (as shown in fig. 17 and 18). At this time, the liquid suction portion 56 is in a state of being torsionally compressed.

The liquid sample collection bottle 10 of the present invention may also be integrated with a detection device (not shown, the specific structure of the detection device is the same as that of the prior art, and the present invention is not described herein again) into a whole (including the two being integrally formed and the two being separately formed and then being assembled into a whole). In other words, the liquid sample collection bottle 10 of the present invention can be formed in a conventional detection apparatus, and the liquid sample collection bottle 10 of the present invention can be used as a liquid sample receiving portion of the detection apparatus. Alternatively, the guide groove 20 according to the present invention is provided on the cylindrical inner wall of the liquid sample receiving portion of the conventional detecting device, and the guide groove 20 is engaged with the guide rail 58 at the lower end of the sampler 10 to guide the liquid sucking portion 56 of the sampler 10 to be squeezed, thereby increasing the liquid sample removal rate of the liquid sucking portion 56. That is, the liquid sample collection bottle 10 of the present invention may be a bottle-shaped structure that is independent of the liquid sample collection bottle, or may be a structure that is a part of a sample receiving portion formed in the detection device.

The invention III: method for collecting liquid sample

The present invention also provides a method of collecting a liquid sample by providing a liquid sample collection member 10 and a sampler 50, with a tilt and screw down guide mechanism between the liquid sample collection member 10 and the sampler 50 for guiding the sampler 50 to screw down within the liquid sample collection member 10. The method comprises the following steps:

A. causing liquid drawing portion 56 of sampler 50 to draw a predetermined number of liquid samples;

B. inserting the lower end 54 of the shaft of the sampler into the liquid sample collection member 10 to form the guide mechanism therebetween;

C. pushing the sampler 50 to move downwards, and leading the liquid drawing part 56 of the sampler to spirally descend in the liquid sample collecting bottle 10 under the guidance of the guide mechanism, so as to screw and squeeze the liquid drawing part 56 of the sampler until the liquid drawing part 56 is fully screwed and squeezed;

D. the sampler 50 is reversely swirled up, the sampler 50 is removed, and the liquid sample collection bottle 10 is sealed.

As a further modification, the guide mechanism includes a guide rail 20 and a guide groove 58 (or a guide groove 20 and a guide rail 58) provided at the lower ends of the shaft portions of the liquid sample collecting member 10 and the sampler 50, respectively.

The invention four: detection device

FIGS. 33 and 34 show the usage status and the detection result of a detection device according to the present invention. The testing device 200 comprises a collecting device 100 (the structure is as described in the second invention, the difference is that a cap 11 is added) for collecting the liquid sample and a testing plate 210. The collection device 100 for collecting a liquid sample includes a liquid sample collection member 10 and a sampler 50, wherein the liquid sample collection member 10 includes a cylindrical inner wall 14, the sampler 50 includes a rod portion 52 and a liquid drawing portion 56, and the liquid drawing portion 56 is connected to a lower end 54 of the rod portion. The cylindrical inner wall 14 is provided with a guide groove 20 inclined from top to bottom and spirally descending, and the rod portion lower end 54 is provided with a guide rail 58. After the lower end 54 of the rod portion of the sampler is inserted into the liquid sample collection member 10, the guide rail 58 at the lower end of the rod portion is engaged with the guide groove 20 of the cylindrical inner wall, so as to guide the lower end 54 of the rod portion of the sampler to spirally descend in the liquid sample collection member 10, thereby squeezing the liquid-drawing portion 56 of the sampler. The detection board 210 includes a detection part 220, and the detection part 220 is used to give detection result information. Detection plate 210 is free of sample application area 212 and detection result display area 214 to facilitate viewing of the detection results. The detection section 220 has T and C lines in the detection result display area 214, which are not visible before detection. During a period of time after detection, the following generally occur: A. if the C line is not displayed (no matter whether the T line is displayed or not), the detection is failed; B. c lines are displayed, but T lines are not displayed, and the detection result is negative; C. when both the C line and the T line are displayed, the detection result is positive, the T line is strong positive when the T line is thick, and the T line is weak positive when the T line is light. In the embodiment shown in FIGS. 33 and 34, the liquid sample collecting member 10 and the test plate 210 or a part of the test plate 210 are separate independent elements, and the structure of the liquid sample collecting member 10 and the sampler 50 is substantially the same as that of "invention two".

Fig. 35 and 36 show the usage status and the detection result of another detection device 200 of the present invention. This embodiment differs from the embodiment shown in fig. 33 and 34 in that the liquid sample collection member 10 is attached to the detection plate 210 or some component of the detection plate 210. The liquid sample collection member 10 and the detection plate 210 or a member of the detection plate 210 may be separately manufactured and then assembled together, or may be integrally formed with the detection plate 210 or a member of the detection plate 210 and then assembled together to form a single unit. The cylindrical inner wall 14 of the liquid sample collecting member 10 is provided with a guide groove 20 which is inclined from top to bottom and spirally descends. The lower end 54 of the rod portion is provided with a guide rail 58. After the lower end 54 of the rod portion of the sampler is inserted into the liquid sample collection member 10, the guide rail 58 at the lower end of the rod portion is engaged with the guide groove 20 of the cylindrical inner wall, so as to guide the lower end 54 of the rod portion of the sampler to spirally descend in the liquid sample collection member 10, thereby squeezing the liquid-drawing portion 56 of the sampler. The basic principle and the specific structure are disclosed as 'invention two'.

Experiment of

The present invention collects saliva of a human using a sponge stick as an example of the sampler 50, and the advantageous effects of the present invention are examined by a comparative experiment.

The experimental method comprises the following steps:

the method comprises the following steps: the sponge sticks of 10 fundamental inventions are sequentially marked as 1-10, the weight of each sponge stick before liquid drawing is respectively weighed by an electronic scale accurate to one thousandth of gram, and the weight is recorded in a column A (the weight unit is gram, the same below) in the following table;

step two: collecting saliva of 10 volunteers with the above 10 sponge sticks, weighing the weight of each sponge stick after the sponge head of the sponge stick is saturated, and recording in column B of the following table;

step three: inserting the 10 sponge sticks into the existing liquid sample collecting bottle respectively, vertically pressing the sponge heads downwards, weighing the sponge heads after fully squeezing out saliva on the sponge heads, and recording the weight in column C in the following table;

step four: respectively inserting the 10 sponge sticks after the third step into the liquid sample collecting bottle, fully extruding saliva on the sponge heads by using the method of the invention, respectively weighing the sponge heads, and recording the weight in a column D in the following table;

step five: the liquid draw-up amount (E), the sample extrusion amount (F) by the conventional method, the sample extrusion amount (G) by the conventional method, the sample extrusion percentage (H) by the conventional method, the sample extrusion percentage (I) by the conventional method, and the percentage increase (J) of the present invention with respect to the conventional method were calculated by the following formulas.

Step six: the average of the percent improvement of the present invention over the current method was calculated.

TABLE I, comparative experimental data of existing sampling method and sampling method of the present invention

According to the experimental data in the table one, the sampling amount of the sampling method of the present invention is improved by 28.67% on average compared with the sampling amount of the current sampling method. The experiment shows that the sampling device and the sampling method have prominent beneficial effects.

In the biological sample inspection program, the sample collection bottle is matched with the sampling rod, so that samples in the sampling rod can be fully collected, and pretreatment steps such as sample cracking and the like can be synchronously completed. The utilization rate of the sampling specimen is improved in detection items such as allergens, tumor markers, gonad markers, alpha-functional markers, infectious disease markers, diabetes markers, liver fibrosis markers and the like which need to be sampled by a sampling rod.

Claims

1. The utility model provides a collection device of collection liquid sample, includes liquid sample collection bottle and sampler, and wherein liquid sample collection bottle is including having the open-ended bottle, and the bottle includes inner wall and outer wall, and the inner wall includes the cylinder portion, and the sampler includes pole portion and draws liquid portion, draw the lower extreme that liquid portion is connected to pole portion, its characterized in that: the cylindrical part of the inner wall of the bottle body is provided with a guide groove which extends downwards from the opening, the guide groove comprises a part which inclines from top to bottom and spirally descends, the lower end of the rod part is provided with a guide rail, after the lower end of the rod part of the sampler is inserted into the liquid sample collecting bottle, the guide rail at the lower end of the rod part is matched with the guide groove of the inner wall of the bottle body, and the lower end of the rod part of the sampler is guided to spirally descend in the liquid sample collecting bottle.

2. A collection device for collecting a liquid sample according to claim 9, wherein: after the inner wall of the bottle body of the liquid sample collecting bottle is unfolded, the guide groove comprises a vertical part and an inclined part from top to bottom.

3. A collection device for collecting a liquid sample according to claim 10, wherein: after the inner wall of the bottle body of the liquid sample collecting bottle is unfolded, the inclined part of the guide groove is a straight line or a smooth curve.

4. A collection device for collecting a liquid sample according to claim 11, wherein: the slope of the smooth curve decreases from top to bottom in sequence.

5. A collection device for collecting a liquid sample according to any of claims 10-12, wherein: the guide groove further includes a horizontal surrounding portion communicating with a bottom of the inclined portion of the guide groove.

6. A collection device for collecting a liquid sample according to any of claims 9-12, wherein: the liquid sampling device is characterized by further comprising a base plate placed in the liquid sample collecting bottle, wherein the base plate is provided with a through hole for the liquid sample to pass through, and the liquid drawing part of the sampler is further screwed and squeezed in the process that the lower end of the rod part of the sampler spirally descends in the liquid sample collecting bottle.

7. A collection device for collecting a liquid sample according to claim 14, wherein: the base plate and the liquid drawing part of the sampler are provided with bulges and depressions which are matched with each other.

8. A collection device for collecting a liquid sample according to claim 15, wherein: the bottom of the liquid-drawing portion is hardened to have higher hardness than other portions of the liquid-drawing portion, thereby being more favorable for reducing slippage between the bottom of the liquid-drawing portion and the upper surface of the pad.

9. A collection device for collecting a liquid sample according to any of claims 9-13, wherein: the guide groove spirally descends one sixth to one week of the inner wall of the bottle body of the liquid sample collecting bottle.

10. The detection device comprises a collection device for collecting the liquid sample and a detection plate, wherein the collection device for collecting the liquid sample comprises a liquid sample collection part and a sampler, the liquid sample collection part comprises a cylindrical inner wall, the sampler comprises a rod part and a liquid drawing part, and the liquid drawing part is connected to the lower end of the rod part, and the detection device is characterized in that: the cylindrical part of the inner wall of the bottle body is provided with a guide groove extending downwards from the opening, the guide groove comprises a part which inclines from top to bottom and spirally descends, the lower end of the rod part is provided with a guide rail, after the lower end of the rod part of the sampler is inserted into the liquid sample collecting bottle, the guide rail at the lower end of the rod part is matched with the guide groove of the inner wall of the bottle body, the lower end of the rod part of the sampler is guided to spirally descend in the liquid sample collecting bottle, the detection plate comprises a detection part, and the detection part is used for giving out detection result information.

11. The detection device of claim 18, wherein: the liquid sample collection member and the detection panel or a component of the detection panel are separate and distinct elements from one another.

12. The detection device of claim 18, wherein: the liquid sample collection member is mounted to the detection plate or a component of the detection plate.