WO2023088400A1

WO2023088400A1 - Liquid sample collection bottle, liquid sample collection device, and detection device

Info

Publication number: WO2023088400A1
Application number: PCT/CN2022/132719
Authority: WO
Inventors: 王中平
Original assignee: 艾康生物技术(杭州)有限公司
Priority date: 2021-11-19
Filing date: 2022-11-18
Publication date: 2023-05-25
Also published as: CN114160219A

Abstract

A liquid sample collection bottle (10), a liquid sample collection device (100), and a detection device (200). The liquid sample collection device (100) comprises the liquid sample collection bottle (10) and a sampler (50), wherein an inner wall (14) of the liquid sample collection bottle (10) is provided with a guide rail (20) inclined from top to bottom and descending spirally, and a rod part lower end (54) of the sampler (50) is provided with a guide groove (58). After the rod part lower end (54) of the sampler (50) is inserted into the liquid sample collection bottle (10), the guide groove (58) at the rod part lower end (54) and the guide rail (20) on the inner wall (14) of the bottle body work in conjunction to guide the rod part lower end (54) of the sampler (50) to spirally descend in the liquid sample collection bottle (10), and then a liquid drawing part (56) of the sampler (50) is squeezed.

Description

A liquid sample collection bottle, liquid sample collection device and detection device

technical field

The invention belongs to the technical field of medical devices, and further belongs to the field of medical testing, and in particular relates to a liquid sample collection bottle, a liquid sample collection device and a detection device.

Background technique

There are a variety of clinical lateral flow immunoassay devices that can be used to detect the presence of analytes in samples, such as hormones, sugars, adulterants, drugs of abuse (including injecting or smoking drugs), etc. Usually, a liquid sample is collected and added to a lateral flow immunoassay device to detect a certain or certain components in the liquid sample.

Wherein, the liquid sample can be any volume of liquid for testing, inspection or research. The liquid sample can come from any liquid, such as body fluid, such as saliva or blood or urine or other liquids to be tested, such as water samples from pollution sources, liquid samples from beverages, etc.

Liquid samples are typically collected and absorbed using a sampling stick having a collecting element formed of absorbent material such as a pad or sponge, which is attached to one end of a plunger. The collection element allows a sufficient volume of sample liquid to be absorbed from the liquid source, the presence of sample liquid in the collection element causing the interior of the collection element to expand. The collection element typically retains the sample until operation of the collection element releases the sample. During detection, the prior art usually squeezes the liquid sample out of the collection element of the sampling stick, and then adds it to the detection device (such as a lateral flow immunoassay device) for detection; or in some detections , It is also necessary to pretreat the extruded liquid sample with a buffer solution such as mixing/lysing, and then add it to the detection device for detection.

In the existing operation, the collection bottle is used to collect the liquid sample on the collection element, that is, the collection element end of the sampling rod is inserted into the collection bottle, and the collection element is pressed against the side wall or bottom of the collection bottle to make the liquid sample flow out into a collection bottle for sample collection. The problem with existing collection bottles and sampling sticks is that the liquid sample on the collection element of the sampling stick cannot be fully squeezed out, that is, after extrusion, there are still more liquid samples remaining on the collection element, so only There are fewer liquid samples for subsequent detection, so it is easy to cause insufficient liquid sample volume for detection, resulting in test failure or inaccurate test results (especially when the liquid sample is saliva, generally, people or The amount of saliva an animal can release in a short period of time is very limited, which can easily lead to insufficient fluid sample volume). In addition, when there is a buffer in the collection bottle, the collection element will also absorb and take away part of the buffer or the mixture of the liquid sample and the buffer, thereby affecting the accuracy of the detection result.

Contents of the invention

One of the objectives of the present invention is to provide a liquid sample collection bottle for collecting liquid samples of people or animals in view of the deficiencies in the prior art.

Above-mentioned purpose of the present invention is achieved through the following technical solutions:

A liquid sample collection bottle includes a bottle body, the bottle body includes an inner wall and an outer wall, and the inner wall includes a cylindrical part, wherein: the cylindrical part of the inner wall of the bottle is provided with guide rails that incline and spirally descend from top to bottom.

As a further improvement of the present invention, the cross-section of the guide rail is rectangular, triangular, trapezoidal or arc-shaped, and the part with a larger cross-sectional size of the guide rail is located on the inner wall of the bottle body.

As a further improvement of the present invention, after the inner wall of the bottle body is unfolded, the guide rail is a straight line inclined from top to bottom.

As a further improvement of the present invention, after the inner wall of the bottle body is unfolded, the guide rail is a smooth curve inclined from top to bottom.

As a further improvement of the present invention, the slope of the smooth curve decreases sequentially from top to bottom.

As a further improvement of the present invention, it also includes a backing plate placed in the bottle, and the backing plate is provided with through holes for the passage of liquid samples.

As a further improvement of the present invention, the upper surface of the backing plate is provided with protrusions or depressions.

As a further improvement of the present invention, the guide rail spirally descends one-sixth to one week of the inner wall of the bottle.

The second object of the present invention is to provide a liquid sample collection device for collecting human or animal liquid samples in view of the deficiencies in the prior art.

A liquid sample collection device, comprising a liquid sample collection bottle and a sampler, wherein the liquid sample collection bottle includes a bottle body, the bottle body includes an inner wall and an outer wall, the inner wall includes a cylindrical part, the sampler includes a rod part and a liquid extraction part, the The liquid-absorbing part is connected to the lower end of the rod part, wherein: the cylindrical part of the inner wall of the bottle is provided with a guide rail inclined from top to bottom and spirally descending, and the lower end of the rod part is provided with a guide groove, and the lower end of the rod part of the sampler is inserted into the liquid sample After collecting in the bottle, the guide groove at the lower end of the rod cooperates with the guide rail on the inner wall of the bottle to guide the lower end of the rod of the sampler to descend spirally in the liquid sample collection bottle.

As a further improvement of the present invention, the cross-section of the guide rail on the inner wall of the bottle is rectangular, triangular, trapezoidal or arc-shaped, and the larger part of the guide rail cross-section is located on the inner wall of the bottle body.

As a further improvement of the present invention, after the inner wall of the bottle body of the liquid sample collection bottle is unfolded, the guide rail is a straight line inclined from top to bottom.

As a further improvement of the present invention, after the inner wall of the bottle body of the liquid sample collection bottle is expanded, the guide rail is a smooth curve inclined from top to bottom.

As a further improvement of the present invention, it also includes a backing plate placed in the liquid sample collection bottle, the backing plate is provided with a through hole to facilitate the passage of the liquid sample, and the lower end of the rod portion of the sampler spirally descends in the liquid sample collection bottle During the process, the backing plate wrings or squeezes the suction part of the sampler.

As a further improvement of the present invention, the backing plate and the dipping part of the sampler are provided with protrusions and depressions that cooperate with each other.

As a further improvement of the present invention, the bottom of the dipping part is hardened so that it has a higher hardness compared to other parts of the dipping part, which is more conducive to reducing the bottom of the dipping part and the upper surface of the backing plate. slippage between.

As a further improvement of the present invention, the guide rail spirally descends one-sixth to one week of the inner wall of the liquid sample collection bottle.

As a further improvement of the present invention, the cooperating guide rails and guide grooves are a pair, or two, three, four, five or six pairs evenly distributed.

As a further improvement of the present invention, the distance between the bottom of the guide rail and the upper surface of the backing plate is greater than or equal to 2.5 mm and less than or equal to 35.0 mm, and the lower end of the rod portion of the sampler can be snapped on the bottom of the guide rail.

As a further improvement of the present invention, the lower surface of the bottom of the guide rail and the upper surface of the lower end of the rod of the sampler each include a slope parallel to each other. During the process of rotating the sampler in the forward direction, the pair of slopes further guides the The bottom presses down on the lower end of the stem of the sampler.

The third object of the present invention is to provide a method for collecting liquid samples for collecting human or animal liquid samples in view of the deficiencies in the prior art.

A method for collecting a liquid sample. A liquid sample collecting device is provided. The liquid sample collecting device includes a sampler and a liquid sample collecting part. An inclined and spirally descending guide mechanism is provided between the sampler and the liquid sample collecting part. In order to guide the sampler to descend spirally in the liquid sample collecting part, the method includes the following steps:

A. Make the liquid sampling part of the sampler draw a predetermined amount of liquid samples;

B. Insert the suction part of the sampler into the liquid sample collection part, so that the inclined guide groove of the sampler corresponds to the inclined and spiral descending guide rail on the inner wall of the liquid sample collection part;

C. Push the sampler down, and under the guidance of the mutual cooperation between the guide groove and the guide rail, the sampler is spirally descended in the liquid sample collection part, so as to wring or squeeze the liquid-absorbing part of the sampler until the liquid-absorbing part is fully twisted squeeze or squeeze

D. The sampler is reversely spiraled up, the sampler is taken out, and the liquid sample collection part is sealed.

As a further improvement of the present invention, the liquid sample collection device is as disclosed in the second invention.

As a further improvement of the present invention, the distance between the bottom of the guide rail on the inner wall of the liquid sample collecting part and the upper surface of the backing plate is greater than or equal to 2.5 mm and less than or equal to 35.0 mm; between the above steps C and D, when After the upper surface of the lower end of the rod portion of the sampler is lower than the bottom of the guide rail, the step of further rotating the sampler forwardly until the lower end of the rod portion of the sampler is buckled on the bottom of the guide rail is also included.

The fourth object of the present invention is to provide a detection device for collecting liquid samples of humans or animals in view of the deficiencies of the prior art.

A detection device, comprising a liquid sample collection device and a detection plate, the liquid sample collection device includes a liquid sample collection part and a sampler, wherein the liquid sample collection part includes a cylindrical inner wall, the sampler includes a rod part and a liquid suction part, the The liquid-absorbing part is connected to the lower end of the rod part, wherein: the inner wall of the cylinder is provided with a guide rail inclined and spirally descending from top to bottom, and the lower end of the rod part is provided with a guide groove, and the lower end of the rod part of the sampler is inserted into the liquid sample collecting part Finally, the guide groove at the lower end of the rod cooperates with the guide rail on the cylindrical inner wall to guide the lower end of the rod of the sampler to spirally descend in the liquid sample collection part, and then twist or squeeze the liquid-absorbing part of the sampler. The detection board includes a detection plate. Component, the detection component is used to give the detection result information.

As a further improvement of the present invention, the liquid sample collecting part and the detection plate or parts of the detection plate are independent components separated from each other.

As a further improvement of the present invention, the liquid sample collection component is mounted on the detection board or a component of the detection board.

Beneficial effect

In the present invention, a guide rail inclined from top to bottom and spirally descending is arranged on the inner wall of the liquid sample collection bottle, and a guide groove is arranged at the lower end of the rod part of the sample. , under the mutual cooperation of the guide rail and the guide groove, the lower end of the rod part of the guide sampler drives the liquid extraction part to perform a spiral downward movement in the sampling bottle, and then produces a squeezing effect similar to wringing a wet towel on the liquid absorption part, thereby fully Squeeze out the liquid sample drawn by the liquid absorbing part. Compared with the prior art, the present invention has a higher liquid sample extrusion rate, and can squeeze out more liquid samples for detection when the same liquid sample is drawn by the suction part, so that it is easier to meet the detection requirements. The minimum amount of liquid samples, thereby improving the success rate of detection and the accuracy of detection results.

Description of drawings

Fig. 1 is a schematic plan view of a liquid sample collection bottle of the present invention.

FIG. 2 is a schematic cross-sectional view along line A-A of FIG. 1 .

Fig. 3 is a schematic plan view of the backing plate in the liquid sample collection bottle of the present invention.

FIG. 4 is a top view of FIG. 1 .

Fig. 5 is a schematic plan view of the liquid sample collection device of the present invention (the sampler is not inserted into the liquid sample collection bottle).

FIG. 6 is a schematic partial cross-sectional view along the line A-A of FIG. 5 .

Figure 7 is similar to Figure 5, but with the sampler inserted into the liquid sample collection bottle in its initial position (the dip has not been squeezed).

FIG. 8 is a schematic cross-sectional view along line A-A of FIG. 7 .

FIG. 9 is a partially enlarged view of part "A" in FIG. 8 .

10-12 are schematic diagrams of modified designs of FIG. 9 .

Fig. 13 is a partially developed plan view of the cylindrical part of the inner wall of the liquid sample collection bottle of the present invention.

FIG. 14 is a schematic diagram of a modified design of FIG. 13 .

Figure 15 is similar to Figure 7, but with the sampler inserted into the final position in the liquid sample collection bottle (with the dip fully squeezed).

Fig. 16 is a schematic cross-sectional view along the line A-A of Fig. 15 .

FIG. 17 is a schematic diagram of a modified design of FIG. 16 .

FIG. 18 is an enlarged schematic view of part “B” in FIG. 17 .

Fig. 19 is another partial angular sectional view of the cooperation between the guide rail on the liquid sample collection bottle and the lower part of the sampler.

Figure 20 is similar to Figure 19, but the sampler continues to twist a certain angle on the basis of Figure 19.

Fig. 21 is similar to Fig. 8, showing another modified design of the present invention.

FIG. 22 is an enlarged schematic view of part “C” in FIG. 21 .

FIG. 23 is a modified design diagram of FIG. 22 .

24-29 are six specific embodiments of the cooperation between the guide rail and the guide groove of the liquid sample collection device of the present invention.

Fig. 30 is a schematic diagram of the use state of the detection device of the present invention.

FIG. 31 is a schematic diagram of the detection results of the detection board shown in FIG. 30 .

Fig. 32 is a schematic diagram of the use state of another embodiment of the detection device of the present invention.

FIG. 33 is a schematic diagram of the detection results of the detection board shown in FIG. 32 .

Detailed ways

The present invention is described in further detail with reference to the accompanying drawings and specific embodiments.

The above specific embodiments are used to explain the present invention, and are only preferred embodiments of the present invention, rather than limiting the present invention. Within the spirit of the present invention and the protection scope of the claims, any modification, equivalent replacement, Improvements and the like all fall within the protection scope of the present invention.

Terminology Explanation

If the patent specification (including claims and abstract) of the present invention explains the terms used, the interpretation of this patent specification shall prevail, and if there is no explanation, it shall be the general meaning in the technical field.

Cylinder: The cylinder of the present invention refers to a hollow cylinder or hollow barrel whose cross-section is circular or approximately circular, and the areas of each cross-section are equal or approximately equal; the cylinder of the present invention also includes cut Hollow cone with truncated apex, that is, the longitudinal section through its axis is an isosceles trapezoid or approximately isosceles trapezoid, but the angle between the hypotenuse and the height of the trapezoid is preferably not more than fifteen degrees, but Nor is it limited to fifteen degrees. In a word, as long as those skilled in the art think that the purpose of the present invention can be achieved and the technical problems to be solved by the present invention are solved, the hollow column or hollow cone that achieves the beneficial effects of the present invention are all included in the cylinder of the present invention. In the range.

Cylindrical part: The part whose shape or structure is a cylinder of the present invention.

Liquid sample collection bottle: also known as a liquid sample collection part, refers to a bottle-shaped object, a cup-shaped object, a cylindrical object, and any shape or part of an object with a cylindrical inner wall for collecting liquid samples.

Spiral descent: also known as spiral descent, that is, on the inner wall of the cylinder, it gradually descends in the form of a spiral from a high place to a low place, forming a three-dimensional smooth curve trajectory. The spiral can be from a fraction of a turn to a turn or several turns.

Rectangle, triangle, trapezoid: including not only the rectangle, triangle, and trapezoid in mathematical concepts, but also the approximate rectangle, triangle, and trapezoid.

Forward: refers to the direction opposite to the reverse, but forward does not specifically refer to the clockwise direction, but refers to one of the clockwise or counterclockwise directions. When the forward direction refers to the clockwise direction, the reverse direction refers to the counterclockwise direction; otherwise, when the forward direction refers to the counterclockwise direction, the reverse direction refers to the clockwise direction.

Squeeze: Squeeze while twisting, that is, the two ends of the object rotate in opposite directions when they are close to each other, producing an effect similar to wringing a wet towel.

The liquid-absorbing part is fully squeezed: it means that under normal manpower, the liquid-absorbing part is squeezed to achieve the effect of basically draining the liquid in the liquid-absorbing part (the liquid discharge rate is obviously higher than that of the prior art) .

Please refer to Fig. 1 and Fig. 2, the present invention provides a liquid sample collection bottle 10, which is used to collect liquid samples such as saliva, blood, urine, sweat, etc. (including immunological detection methods and non-immunological detection methods) to detect the content of a certain substance or substances in these liquid samples, so as to obtain the physiological parameter indicators of the tested human body or animal. Taking the detection of new coronavirus pneumonia as an example, scientists have developed products and detection methods that use human or animal saliva (including processed) as samples to determine whether the human body or animal that provided the saliva sample is infected with the new coronavirus. Coronavirus pneumonia. One of the important links of the detection method is how to collect the saliva of the human body or animal to be tested. The object of the present invention is to provide a novel liquid sample collection bottle for collecting human or animal saliva, a liquid sample collection device, a detection device, and a method for collecting liquid samples using the liquid sample collection device. The invention can be used for the detection of novel coronavirus pneumonia.

Invention 1: Liquid sample collection bottle

Referring to Fig. 1 and shown in Fig. 2, the liquid sample collection bottle 10 of the present invention comprises a bottle body 12, and the bottle body 12 includes an inner wall 14 and an outer wall 16, and the inner wall 14 includes a cylindrical portion 18 (that is, at least a part of the inner wall 14 is a cylinder) structure), the cylindrical part 18 is provided with a guide rail 20 that inclines and descends spirally from top to bottom. The guide rail 20 protrudes from the inner wall 14 to the centerline of the cylinder for an appropriate distance, and is attached to the inner wall 14 as a helical disc. Please refer to Fig. 9-12, the cross-section of the guide rail 20 is rectangular (including square), triangular, trapezoidal or arc-shaped (including part circular or part oval), and the larger part of the cross-section of the guide rail 20 (As shown by the dotted lines in FIGS. 9-12 ) located on the inner wall 14 of the bottle body 12 (that is, in the direction from the inner wall 14 to the centerline of the cylinder, the cross-sectional dimensions of the guide rails 20 are equal or gradually smaller).

Please refer to FIG. 13 , in one embodiment, after the cylindrical portion 18 of the inner wall 14 of the bottle body 12 is unfolded, the guide rail 20 on the inner wall 14 is in an inclined straight line from top to bottom. The included angle "A" between the inclined straight line and the horizontal plane is greater than or equal to 30 degrees and less than or equal to 75 degrees, and the preferred included angle "A" is 60 degrees and 45 degrees. As shown in FIG. 14 , in another embodiment, after the inner wall 14 of the bottle body 12 is unfolded, the guide rail 20 on the inner wall 14 is in the shape of an inclined smooth curve from top to bottom. The slope of the smooth curve decreases successively (that is, the angle between the tangent of the smooth curve and the horizontal plane decreases gradually). In this embodiment, preferably, the included angle "C" between the tangent line at the top of the guide rail 20 and the horizontal plane is 75 degrees, and the included angle "B" between the tangent line at the bottom of the guide rail 20 and the horizontal plane is 30 degrees.

As shown in FIG. 2 , the liquid sample collection bottle 10 further includes a backing plate 22 placed in the bottle body 12 . The backing plate 22 is provided with several through holes 24 to facilitate the flow of liquid samples. The backing plate 22 is in the shape of a disc with a thickness of about 2.0-5.0 mm and is usually made of hard plastic. In the embodiment shown in FIGS. 1 and 2 , the bottle body 12 includes an upper part and a lower part. The size of the upper part is obviously larger than that of the lower part. A shoulder 26 is formed at the junction of the two parts. The backing plate 22 is placed on and supported by the shoulder 26 . In one embodiment, when the area of the shoulder 26 is twice or more than the cross-sectional area of the inner diameter of the lower part of the bottle body, the backing plate 22 can also be omitted, that is, the backing plate 22 is not necessary. FIG. 3 is a top plan view of the backing plate 22 , and FIG. 4 is a top plan view of the backing plate 22 after being placed in the bottle body 12 . In one embodiment, a depression 30 (as shown in FIG. 22 , the depression may also be a through hole 24 as shown in FIG. 3 ) or a protrusion 28 (as shown in FIG. 23 ) is provided on the upper surface of the backing plate 22 . The guide rail 20 is at least inclined and spirally descends one-sixth of the circle of the inner wall 18 of the bottle, but preferably no more than one circle (not necessarily no more than one circle, in some cases, it can also be several circles). One guide rail 20 (as shown in Figure 24) can be set on the inner wall 18 of the cylinder part, and two, three, four, five and six guide rails 20 can also be evenly arranged (as shown in Figures 25-29) , but the number of guide rails 20 should not limit the protection scope of the present invention.

Invention 2. Liquid sample collection device

5-8, the present invention also provides a liquid sample collection device 100, which includes the liquid sample collection bottle 10 as described in the first invention (in order to avoid repetition, the content in the first invention All are incorporated into the second invention, and the specific structure of the liquid sample collection bottle 10) and the sampler 50 will not be described in detail in the second invention. The sampler 50 includes a stem 52 and a dip 56 connected to a lower end 54 of the stem. The liquid-absorbing part 56 is made of a material with excellent water absorption and drainage properties, such as sponge, absorbent cotton, gauze, special fiber and the like. Rod 52 is made of hard material, and the lower end 54 of the rod is disc-shaped, and its periphery is provided with guide groove 58, and the shape and size of this guide groove 58 are corresponding to guide rail 20 on the bottle inner wall 14 (Fig. 5 and The guide groove 58 shown in Figure 6 is inclined, and the direction of inclination is consistent with the guide rail 20), the size of the bar lower end 54 is adapted to the space surrounded by the bottle inner wall 14, so that even the guide rail 20 can move in the guide groove 58, the guide rail 20 and the guide groove 58 can cooperate with each other to play the role of guiding the lower end 54 of the rod portion to spiral down. After the rod lower end 54 of the sampler 50 is inserted in the liquid sample collection bottle 10, the guide groove 58 of the rod lower end 54 cooperates with the guide rail 20 on the bottle inner wall 14, and the rod lower end 54 of the guide sampler 50 is in the Spiral descending inside liquid sample collection bottle. When the bottom of the dipping portion 56 presses against the upper surface of the pad portion 22, the surface friction between the two reduces the relative sliding of the bottom of the dipping portion 56 relative to the pad portion 22, while the guide rail 20 guides the lower end 54 of the stem portion. Drive the upper part of the liquid-absorbing part 56 to continue to twist and descend, so that the lower end 54 of the rod part twists the liquid-absorbing part 56 while squeezing downward, and produces the effect of squeezing the liquid-absorbing part 56 (similar to wringing a wet towel), and then more thoroughly The liquid sample is discharged from the drawing part 56 into the liquid sample collection bottle 10, and mixed with the lysate 60 in the collection bottle (as shown in FIG. 8 ) to form a mixed solution 62 of the lysate and the liquid sample (as shown in FIG. 16 ). .

As described in the first invention, after the cylinder portion 18 of the inner wall 14 of the bottle body 12 is unfolded, the guide rail 20 on the inner wall 14 is in the shape of an inclined straight line or a smooth curve from top to bottom. Please refer to FIG. 14 , the smooth curved guide rail is beneficial to the guide rail 20 and the guide groove 58 to guide the lower end 54 of the rod part to accelerate the descending speed at the beginning of the descending stage, thereby exerting a stronger downward extrusion on the liquid extraction part, while in the When approaching the end of the descent, the guide rail 20 and the guide groove 58 guide the lower end 54 of the rod part to produce a stronger twist, thereby producing a better wringing effect on the liquid drawing part 56, and squeezing out more liquid samples from the liquid drawing part 56 .

As shown in Figures 21-23, in order to reduce the relative sliding between the bottom of the liquid absorbing portion 56 and the upper surface of the backing plate 22, the upper surface of the backing plate 22 and the bottom of the liquid absorbing portion 56 of the sampler are provided with mutual cooperation. The protrusions 28 and the depressions 30 cooperate with each other to reduce the relative slippage between the bottom of the dipping part 56 and the upper surface of the backing plate 22, and enhance the wringing effect on the dipping part 56. Both can be provided with depression 30 (this depression also can be through hole 24) on the upper surface of backing plate 22 and be provided with protrusion 28 (as shown in Figure 22) at the bottom of the dipping part 56 of sampler, also can be on backing plate The upper surface of the sampler 22 is provided with a protrusion 28 and a depression 30 (as shown in Figure 23 ) at the bottom of the dipping portion 56 of the sampler, or the bottom of the dipping portion 56 and the upper surface of the backing plate 22 are made rough. , to increase the friction between the two. Preferably, the bottom of the dipping portion 56 is hardened so that it has a higher hardness compared to other parts of the dipping portion, so that it is more conducive to reducing the gap between the bottom of the dipping portion 56 and the upper surface of the backing plate 22. The sliding between them improves the squeezing effect on the liquid-absorbing part 56, and further improves the discharge rate of the liquid sample from the liquid-absorbing part 56.

As shown in FIG. 24 , the guide rail 20 and the guide groove 58 that cooperate with each other form a pair. As shown in Figures 25-29, in order to improve the stability of the guide, the guide rails 20 and the guide grooves 58 that cooperate with each other can also be two pairs, three pairs, four pairs, five pairs or six pairs that are evenly distributed, so that the pairing can be improved. The wringing effect of the liquid drawing part 56 further improves the efficiency of wringing out the liquid sample from the liquid drawing part 56 .

As shown in Fig. 2 and Fig. 6, the distance "L" between the bottom of the guide rail 20 and the upper surface of the backing plate 22 is greater than or equal to 2.5 millimeters and less than or equal to 35.0 millimeters, and this distance "L" can make the rod portion of the sampler 50 The lower end 54 is buckled on the bottom of the guide rail 20 (as shown in FIGS. 17 and 18 ). The advantage of such a design is that the liquid-absorbing part 56 can still be kept in a twisted and compressed state after the operator lets go.

As shown in FIGS. 19 and 20 , the lower surface of the bottom of the guide rail 20 and the upper surface of part of the lower end 56 of the rod portion of the sampler 50 are a pair of

inclined surfaces

32 and 34 that cooperate with each other. In the process of rotating the sampler in the forward direction (as shown by the arrows in Figures 19 and 20), the pair of inclined surfaces have a guiding effect, and make the bottom of the guide rail 20 further press the lower end 54 of the rod portion of the sampler 50 downward. , still make the lower end of the rod portion of the sampler be squeezed by the bottom of the guide rail 20 after the operator lets go.

Invention 3: A method for collecting liquid samples

The present invention also provides a method for collecting a liquid sample. The method provides the liquid sample collecting part 10 and the sampler 50 as disclosed in the first and second inventions above. The guide mechanism for spiral descent is used to guide the sampler 50 to descend spirally in the liquid sample collection part 10 . The method comprises the steps of:

A. Make the liquid sampling part 56 of the sampler 50 draw a predetermined amount of liquid samples;

B. Insert the lower end 54 of the rod part of the sampler into the liquid sample collecting part 10, so that the guiding mechanism is formed between the two;

C. Push the sampler 50 down, and under the guidance of the guide mechanism, make the liquid-absorbing part 56 of the sampler spirally descend in the liquid sample collection bottle 10, thereby twisting or squeezing the liquid-absorbing part 56 of the sampler, until the dipping part 56 is fully twisted or squeezed;

D. The sampler 50 is spiraled upward in reverse, the sampler 50 is taken out, and the liquid sample collection bottle 10 is sealed.

As a further improvement, the guide mechanism includes guide rails 20 and guide grooves 58 (or guide grooves 20 and guide rails 58 ) respectively arranged at the lower ends of the rods of the liquid sample collecting part 10 and the sampler 50 .

As a further improvement, the distance between the bottom of the guide rail 20 on the inner wall of the liquid sample collection bottle 10 and the upper surface of the backing plate 22 is greater than or equal to 2.5 millimeters and less than or equal to 35.0 millimeters; between the above steps C and D, when the sampler After the upper surface of the lower end 54 of the rod portion is lower than the bottom of the guide rail 20, the method further includes the step of further rotating the sampler 50 clockwise until the lower end 54 of the rod portion of the sampler is buckled on the bottom of the guide rail 20. In this way, the lower end 54 of the rod part of the sampler can be kept at the bottom of the guide rail 20, thereby more fully wringing or squeezing the liquid-absorbing part 56, and improving the discharge rate of the liquid sample.

Invention 4: A detection device

Figures 30 and 31 show the use status and detection results of a detection device of the present invention. The detection device 200 includes a liquid sample collection device 100 (the structure is as described in "Invention 2", the difference is that a cap 11 is added) and a detection plate 210 . The liquid sample collection device 100 comprises a liquid sample collection part 10 and a sampler 50 (the sampler 50 is omitted in FIG. , the dipping portion 56 is connected to the lower end 54 of the rod portion. The cylindrical inner wall 14 is provided with a guide rail 20 inclined from top to bottom and spirally descending, and the lower end 54 of the rod part is provided with a guide groove 58 inclined in the same direction as the guide rail 20 . After the lower end 54 of the rod portion of the sampler is inserted into the liquid sample collecting part 10, the guide groove 58 at the lower end of the rod part cooperates with the guide rail 20 on the inner wall of the cylinder to guide the lower end 54 of the rod part of the sampler to descend spirally in the liquid sample collecting part 10. , and then wring or extrude the dipping portion 56 of the sampler. The detection board 210 includes a detection component 220, and the detection component 220 is used for giving detection result information. The test board 210 is provided with a sample loading area 212 and a test result display area 214 for observing the test results. The detection part 220 has a T line and a C line in the detection result display area 214, and the T line and the C line are invisible before detection. Within a period of time after the detection, the following situations generally occur: A. If the C line is not displayed (whether the T line is displayed or not), it means that the detection has failed; B. If the C line is displayed but the T line is not displayed, it means the detection The result is negative; C, showing both the C line and the T line, it means that the test result is positive, and the T line is thick, it is strong positive, and the T line is weak, it is weak positive. In the embodiment shown in Figures 30 and 31, the liquid sample collection part 10 and the detection plate 210 or parts of the detection plate 210 are independent components separated from each other, and the structures of the liquid sample collection part 10 and the sampler 50 are similar to those of "Invention 2" "basically the same.

32 and 33 show the usage status and detection results of another detection device 200 of the present invention. The difference from the embodiment shown in FIGS. 30 and 31 is that the liquid sample collecting part 10 is mounted on the detection board 210 or a certain part of the detection board 210 . The liquid sample collection part 10 and the detection board 210 or a certain part of the detection board 210 can be manufactured separately and then assembled together, or can be integrally formed with the detection board 210 or a certain part of the detection board 210 and then assembled together , forming a whole. The cylindrical inner wall 14 of the liquid sample collecting part 10 is provided with a guide rail 20 inclined and spirally descending from top to bottom. The lower end 54 of the rod portion is provided with a guide groove 58 inclined in the same direction as the guide rail 20 . After the lower end 54 of the rod portion of the sampler is inserted into the liquid sample collecting part 10, the guide groove 58 at the lower end of the rod part cooperates with the guide rail 20 on the inner wall of the cylinder to guide the lower end 54 of the rod part of the sampler to descend spirally in the liquid sample collecting part 10. , and then wring or extrude the dipping portion 56 of the sampler. The basic principle and specific structure are as disclosed in "Invention 2".

experiment

The present invention uses a sponge stick as an example of the sampler 50 to collect human saliva, and uses the method of comparative experiment to check the beneficial effects of the present invention.

experimental method:

Step 1: Use the sponge sticks of the 10 fundamental inventions to be numbered 1-10 in sequence, and use an electronic scale accurate to one-thousandth of a gram to weigh the weight of each sponge stick before drawing liquid, and record it in "A" in the table below " column (the unit of weight is grams, the same below);

Step 2: Use the above 10 sponge sticks to collect the saliva of 10 volunteers. After the sponge head of the sponge stick is absorbed and saturated, weigh the weight of each sponge stick after absorbing the liquid, and record it in the following table. Column "B";

Step 3: Insert the above 10 sponge sticks into the existing liquid sample collection bottles, squeeze the sponge head vertically, fully squeeze out the saliva on the sponge head, and weigh the sponge head respectively , recorded in column "C" in the table below;

Step 4: insert the 10 sponge sticks after performing step 3 into the liquid sample collection bottle of the present invention respectively, after fully extruding the saliva on the sponge head with the method of the present invention, weigh the weight of the sponge head respectively , recorded in column "D" in the table below;

Step 5: use the formula in the following table to calculate respectively the amount of liquid drawn (E) of these 10 sponge sticks, the sample extrusion amount (F) using the existing method, and the sample extrusion amount (G) using the method of the present invention. ), adopt the sample extrusion percentage (H) of existing method, adopt the sample extrusion percentage (I) of the method of the present invention and the present invention improve percentage (J) relative to present method.

Step 6: Calculate the average value of the percentage improvement of the present invention relative to the current method.

Table one, the comparative experimental data of existing sampling method and sampling method of the present invention

According to the experimental data in Table 1, the sampling amount of the sampling method of the present invention is improved by 31.30% on average compared with the current sampling method. This experiment shows that the beneficial effects of the sampling device and sampling method of the present invention are very prominent.

In the biological sample testing procedure, the sample collection bottle of the present invention cooperates with the sampling stick to fully collect the samples in the sampling stick, and can simultaneously complete the pretreatment steps such as sample cracking. In allergens, tumor markers, gonad markers, thyroid function markers, infectious disease markers, diabetes markers, liver fibrosis markers and other testing items that require sampling sticks to collect samples, improve the utilization rate of sampling specimens .

Claims

A liquid sample collection bottle, including a bottle body, the bottle body includes an inner wall and an outer wall, and the inner wall includes a cylindrical part, and is characterized in that: the cylindrical part of the inner wall of the bottle is provided with guide rails that incline and spiral down from top to bottom.
The liquid sample collection bottle according to claim 1, characterized in that: the cross-section of the guide rail is rectangular, triangular, trapezoidal or arc-shaped, and the part with a larger cross-sectional size of the guide rail is located on the inner wall of the bottle body.
The liquid sample collection bottle according to claim 1, wherein after the inner wall of the bottle body is unfolded, the guide rail is a straight line inclined from top to bottom.
The liquid sample collection bottle according to claim 1, wherein after the inner wall of the bottle body is unfolded, the guide rail is a smooth curve inclined from top to bottom.
The liquid sample collection bottle according to claim 4, wherein the slope of the smooth curve decreases successively from top to bottom.
The liquid sample collection bottle according to any one of claims 1-5, characterized in that it further comprises a backing plate placed in the bottle, and the backing plate is provided with a through hole for the passage of the liquid sample.
The liquid sample collection bottle according to claim 6, characterized in that: the upper surface of the backing plate is provided with protrusions or depressions.
The liquid sample collection bottle according to any one of claims 1-5, characterized in that: the guide rail is inclined and spirally descends one-sixth to one week of the inner wall of the bottle.
A liquid sample collection device, comprising a liquid sample collection bottle and a sampler, wherein the liquid sample collection bottle includes a bottle body, the bottle body includes an inner wall and an outer wall, the inner wall includes a cylindrical part, the sampler includes a rod part and a liquid extraction part, the The liquid-absorbing part is connected to the lower end of the rod part, and it is characterized in that: the cylinder part of the inner wall of the bottle is provided with a guide rail inclined from top to bottom and spirally descending, and the lower end of the rod part is provided with a guide groove, and the lower end of the rod part of the sampler is inserted into the After the liquid sample is collected in the bottle, the guide groove at the lower end of the rod cooperates with the guide rail on the inner wall of the bottle to guide the lower end of the rod of the sampler to descend spirally in the liquid sample collection bottle.
The liquid sample collection device according to claim 9, characterized in that: the cross-section of the guide rail is rectangular, triangular, trapezoidal or arc-shaped, and the part with a larger cross-sectional size of the guide rail is located on the inner wall of the bottle body.
The liquid sample collection device according to claim 9, characterized in that, after the inner wall of the bottle body of the liquid sample collection bottle is unfolded, the guide rail is an inclined straight line from top to bottom.
The liquid sample collection device according to claim 9, characterized in that: after the inner wall of the bottle body of the liquid sample collection bottle is unfolded, the guide rail is a smooth curve inclined from top to bottom.
The liquid sample collection device according to claim 12, wherein the slope of the smooth curve decreases sequentially from top to bottom.
The liquid sample collection device according to any one of claims 9-13, characterized in that: it also includes a backing plate placed in the liquid sample collection bottle, the backing plate is provided with a through hole for the liquid sample to pass through, and When the lower end of the rod part of the sampler is spirally descending in the liquid sample collection bottle, the pad part squeezes the liquid-absorbing part of the sampler.
The liquid sample collecting device according to claim 14, characterized in that: the backing plate and the suction part of the sampler are provided with protrusions and depressions that cooperate with each other.
The liquid sample collection device according to claim 15, wherein the bottom of the liquid-absorbing part is hardened so that it has a higher hardness than other parts of the liquid-absorbing part.
The liquid sample collection device according to claim 16, characterized in that: the distance between the bottom of the guide rail and the upper surface of the backing plate is greater than or equal to 2.5 mm and less than or equal to 35.0 mm, and the lower end of the rod part of the sampler can be stuck Snaps to the bottom of the rail.
The liquid sample collection device according to claim 17, wherein the lower surface of the bottom of the guide rail and the upper surface of the lower end of the rod of the sampler each include a slope parallel to each other, during the process of rotating the sampler in the forward direction , the pair of slopes further guides the bottom of the guide rail to press the lower end of the rod portion of the sampler downward.
A detection device, comprising a liquid sample collection device and a detection plate, the liquid sample collection device includes a liquid sample collection part and a sampler, wherein the liquid sample collection part includes a cylindrical inner wall, the sampler includes a rod part and a liquid suction part, the The liquid-absorbing part is connected to the lower end of the rod, and it is characterized in that: the inner wall of the cylinder is provided with a guide rail inclined from top to bottom and spirally descending, the lower end of the rod is provided with a guide groove, and the lower end of the rod of the sampler is inserted into the liquid sample to collect Inside the part, the guide groove at the lower end of the rod cooperates with the guide rail on the inner wall of the cylinder to guide the lower end of the rod of the sampler to spiral down in the liquid sample collection part. The detection board includes the detection part, which is used to give the test result information .
The detection device according to claim 19, characterized in that the liquid sample collecting part and the detection plate or components of the detection plate are independent components separated from each other.
The detection device according to claim 19, characterized in that the liquid sample collecting part is installed on the detection board or a part of the detection board.