CN114377428A - Method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by applying eutectic solvent - Google Patents
Method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by applying eutectic solvent Download PDFInfo
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Abstract
A method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by using a eutectic solvent belongs to the field of extraction research of flavonoid compounds in rhizoma polygonati, and can solve the problems of high toxicity, poor biodegradability, low extraction efficiency and the like of the existing traditional organic solvent as an extractant. According to the method, a eutectic solvent is combined with an ultrasonic-assisted extraction method to carry out green and efficient extraction on flavonoid compounds in rhizoma polygonati, the extraction conditions are optimized firstly, then the antioxidant activity of the flavonoid compounds in the extracting solution is evaluated, and finally the eutectic solvent in the eutectic solvent extracting solution is recycled. The method adopts the environment-friendly eutectic solvent as the extracting agent, has high extraction efficiency, good oxidation resistance of the extracting solution and good recoverability, can be repeatedly used, and provides a new idea for extracting flavonoid compounds in the sealwort by taking the eutectic solvent as the extracting medium.
Description
Technical Field
The invention belongs to the field of extraction research of flavonoid compounds in rhizoma polygonati, and particularly relates to a method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by using a eutectic solvent.
Background
Rhizoma Polygonati (Polygonatumsibiricum) Is a perennial herb of the liliaceae family, which is a medicinal plant widely distributed in china, japan and korea. Rhizoma Polygonati is rich in flavone, polysaccharide, saponin, and various amino acids essential to human body. Wherein, the flavonoid compound is the main bioactive component in the rhizoma polygonati. The flavonoid compound is a natural compound, is a plant secondary metabolite and is the most abundant pigment in plants. The flavonoid compounds have various biological activities of resisting oxidation, inflammation, bacteria, radiation, cancer, virus, blood pressure and the like. The research on the extraction of the flavonoid compounds in the rhizoma polygonati is of great significance.
The method for effectively extracting the flavonoid compounds in the sealwort from the natural products mainly adopts a solid-liquid extraction process. In the extraction process, it is very important to select a suitable solvent, which greatly affects the extraction yield and composition of the extract. In recent years, conventional organic solvents such as methanol, ethanol, acetone, ethyl acetate, and the like have been widely used to extract bioactive compounds from natural sources. Although the organic solvent has important characteristics of extracting natural products, the organic solvent also has inherent limitations, such as toxicity, flammability, high volatility and poor biodegradability, and the problems that the use of a large amount of the organic solvent causes environmental pollution and the residual organic solvent in the extract cannot be treated are solved. Therefore, great efforts are being made to develop an extraction solvent for green and sustainable extraction of bioactive components from natural products.
Eutectic solvents have attracted wide attention in many fields with their unique advantages as emerging solvents replacing conventional organic solvents. The method has attracted extensive attention in the fields of extraction separation, organic reaction, nano materials and the like. The eutectic solvent has the important characteristics of easy availability of components, simple preparation, low cost, low toxicity, biocompatibility, easy storage, biodegradability, sustainability and the like, is a novel substitute of the traditional organic solvent, and has wide application prospect in the green extraction technology. The eutectic solvent is a transparent and clear mixture prepared by heating and stirring hydrogen bond acceptor and hydrogen bond donor in a certain molar ratio. Common eutectic solvents are the use of choline chloride as a hydrogen bond acceptor and alcohols, acids, sugars, amines as hydrogen bond donors. Nowadays, most of the literatures still mainly use choline chloride as a hydrogen bond receptor to extract bioactive compounds such as active ingredients in flowers, fruits and rhizomes, but the application of various targets is limited due to the single selection of the hydrogen bond receptor. And the choline chloride has the defects of high viscosity, easy crystallization and the like, so that the extraction efficiency is low. In order to further expand the application of the eutectic solvent in the extraction of bioactive compounds and improve the extraction efficiency, the development of a new hydrogen bond receptor for synthesizing the eutectic solvent has important significance. The optimal extraction conditions required by different flavonoid compounds are different, so that the research of carrying out a series of optimization experiments on the extraction conditions of the flavonoid compounds in the rhizoma polygonati is of great significance.
The solvent can be continuously utilized by recycling the solvent, resources are effectively saved, and the development of green chemistry is promoted. The eutectic solvent is introduced as a new solvent and is effective for the extraction of various types of bioactive compounds. Although the eutectic solvent is green, it cannot be effectively recovered by the conventional reduced pressure concentration method due to its low vapor pressure. There is a need for a suitable method of recovering the eutectic solvent. The carbon adsorption material has small particle size, large specific surface area, van der Waals interaction in the structure, good stability and large adsorption capacity. The adsorption method of the carbon adsorption material has high adsorption efficiency, is simple and feasible, and is expected to realize effective recovery of the extractant. Different carbon adsorption materials have different polarities, particle sizes, intermolecular forces and the like, and have different adsorption effects on flavonoid compounds in rhizoma polygonati. Meanwhile, the further green extraction is realized by recycling the extracting agent.
Disclosure of Invention
The invention provides a method for green and high-efficiency extraction of flavonoid compounds in polygonatum sibiricum by using a eutectic solvent, aiming at the problems that the toxicity of the traditional organic solvent as an extractant is high, the biodegradability is poor, the extraction efficiency is low, and different flavonoid compounds need different polarity, intermolecular force, surface tension and other conditions.
The invention adopts the following technical scheme:
a method for green and high-efficiency extraction of flavonoid compounds in rhizoma polygonati by using a eutectic solvent comprises the following steps:
firstly, preparing a eutectic solvent as an extracting agent, taking any one of betaine, L-proline, glucose and choline chloride as a hydrogen bond receptor, taking acetic acid as a hydrogen bond donor, mixing the materials in proportion, and heating and stirring the mixture at 80 ℃ until the mixture becomes uniform, transparent and clear liquid to form the eutectic solvent;
secondly, crushing a rhizoma polygonati sample to obtain rhizoma polygonati powder, weighing the rhizoma polygonati powder in a test tube, adding a prepared eutectic solvent, carrying out ultrasonic extraction, then centrifuging to obtain a flavonoid compound extracting solution in the rhizoma polygonati, establishing a rutin standard curve by taking rutin as a standard substance, and measuring the extraction rate of flavonoid compounds in the rhizoma polygonati;
and thirdly, evaluating the antioxidant activity of the flavonoid compounds in the extracting solution, adsorbing the flavonoid compounds in the extracting solution by adopting a carbon adsorbing material, and performing vortex, centrifugation and filtration to realize the recovery and reutilization of the eutectic solvent of the extracting agent.
Further, in the first step, the hydrogen bond acceptor is betaine, and the molar ratio of the betaine to acetic acid is 1: 4.
Further, the grain size of the sealwort powder in the second step is 0.425 mm.
Further, the ratio of the polygonatum sibiricum powder to the eutectic solvent in the second step is 1:100 g/mL.
Further, the ultrasonic extraction temperature in the second step is 50 ℃, and the extraction time is 30 min.
Further, the rotation speed of the centrifugation in the second step is 4000 rpm, and the centrifugation time is 5 min.
Further, the third step is to evaluate the antioxidant activity of the flavonoid compounds in the extracting solution, including the measurement of DPPH free radical and hydroxyl free radical scavenging capacity.
Further, the carbon adsorbing material in the third step is 100mg of ultra-high capacitance porous carbon.
Further, in the third step, the vortex time is 10 min, the centrifugal speed is 4000 rpm, the centrifugal time is 10 min, and a membrane filter with the pore size of 0.22 μm is adopted for filtration.
The extracted sample rhizoma polygonati is a plant with homology of medicine and food.
The invention has the following beneficial effects:
1. the method for green and efficient extraction of flavonoid compounds in rhizoma polygonati provided by the invention adopts the eutectic solvent as the extracting agent. Betaine, L-proline, glucose and choline chloride are used as hydrogen bond receptors, acetic acid is used as a hydrogen bond donor, the synthesized eutectic solvent can replace traditional toxic extracting agents such as methanol, ethanol, acetone and ethyl acetate, and the extracting agents have toxicity and volatility and are harmful to operators and the environment. The eutectic solvent has the advantages of green color, easy synthesis, low cost, low toxicity and high biodegradability, and meets the requirements of green chemical experiments.
2. Different flavonoid extraction conditions such as different viscosity, polarity and solubility are required, so that experiments for optimizing the types, molar ratios, water contents and the like of eutectic solvents and the types of carbon materials used for recovering flavonoid compounds in rhizoma polygonati are required for the flavonoid compounds in rhizoma polygonati. According to the method, the eutectic solvent synthesized by betaine and acetic acid is finally used as the extracting agent to extract the flavonoid compounds in the sealwort, probably because the eutectic solvent is more similar to the polarity of most flavonoid compounds in the sealwort, forms hydrogen bonds with most flavonoid compounds in the sealwort, has stronger hydrophobic interaction, is closer to the pH value of most flavonoid compounds in the sealwort, and is more beneficial to the extraction of the flavonoid compounds in the sealwort.
3. An environment-friendly eutectic solvent is utilized to extract flavonoid compounds from medicinal and edible plant rhizoma polygonati.
4. The extraction rate of flavonoid compounds in sealwort by using eutectic solvent reaches 25.40 mgg-1Is about eight times of the traditional ethanol extraction method.
5. The oxidation resistance of the eutectic solvent extracting solution obtained by an oxidation resistance test is higher than that of an ethanol extracting solution.
6. In the recovery test of the extracting agent, the ultrahigh-capacitance porous carbon has large specific surface area, small particle size, polarity similar to that of flavonoid compounds in rhizoma polygonati, van der Waals interaction in the structure, good stability, large adsorption capacity and strong adsorption capacity. Therefore, the eutectic solvent can be efficiently recovered by the ultrahigh-capacitance porous carbon, the recovery rate is 95%, and after the recovered eutectic solvent is subjected to ten-time circulating extraction, the extraction rate can reach 93% of the original extraction rate, so that the selected eutectic solvent has good recoverability and reusability.
Drawings
FIG. 1 shows the effect of the eutectic solvent on the extraction rate of flavonoid compounds from Polygonatum sibiricum Red.
FIG. 2 is the effect of the molar ratio of the eutectic solvent on the extraction rate of flavonoid compounds from Polygonatum sibiricum Red.
FIG. 3 is the effect of the water content of the eutectic solvent on the extraction rate of flavonoid compounds from Polygonatum sibiricum Red.
FIG. 4 shows the effect of feed liquid ratio on the extraction rate of flavonoid compounds from Polygonatum sibiricum Red.
FIG. 5 is the effect of extraction temperature on the extraction rate of flavonoids from Polygonati officinalis rhizoma.
FIG. 6 is the effect of extraction time on the extraction rate of flavonoids from Polygonatum sibiricum Red.
FIG. 7 shows the radical scavenging ability of eutectic solvent flavone extract and ethanol flavone extract with different contents.
Fig. 8 shows adsorption ratios of different carbon adsorbents.
FIG. 9 shows the extraction yield of the extractant recycling.
Detailed Description
The invention synthesizes a green high-efficiency eutectic solvent as an extracting agent, firstly optimizes extraction conditions including the type, the molar ratio, the water content, the material-liquid ratio, the extraction temperature and the extraction time of the eutectic solvent, then evaluates the oxidation resistance of an extracting solution, and finally recycles the extracting solvent, aiming at establishing a green high-efficiency method for extracting flavonoid compounds in polygonatum sibiricum by using the eutectic solvent.
For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.
Example 1
The method for extracting flavonoid compounds from rhizoma polygonati by using the eutectic solvent and optimizing the extraction conditions comprises the following steps:
the invention has more influence factors on the extraction rate of the flavonoid compounds in the rhizoma polygonati, and a series of optimization experiments are needed. Therefore, parameters of the type, the molar ratio, the water content, the feed-liquid ratio, the extraction temperature and the extraction time of the eutectic solvent are optimized. The extraction effect of the flavonoid compounds in the rhizoma polygonati is evaluated by taking the extraction rate of the flavonoid compounds in the rhizoma polygonati as an index.
1. Screening of optimal eutectic solvent species
The screening of the optimal eutectic solvent species is the first step of extraction condition optimization, the eutectic solvents are directly used for screening the performance of extracting flavonoid compounds from rhizoma polygonati powder in detail, the research takes betaine, L-proline, glucose and choline chloride as hydrogen bond receptors, acetic acid as hydrogen bond donors, four eutectic solvents (betaine-acetic acid, L-proline-acetic acid, glucose-acetic acid and choline chloride-acetic acid) are prepared as extracting agents, and other extraction conditions are as follows: the water content of the eutectic solvent is 30 percent, and the feed-liquid ratio is 1:100g mL-1The extraction temperature is 50 ℃, and the extraction time is 30 min.The results obtained are shown in FIG. 1. It can be seen that the extraction yield obtained using the eutectic solvent extraction method is generally much greater than that obtained using the ethanol extraction method. In addition, the betaine-acetic acid has the strongest capacity of extracting the flavonoid compounds in the sealwort. This is probably due to the lowest viscosity of betaine-acetic acid, which is advantageous for the extraction of flavonoids from polygonatum sibiricum. The viscosities of four different eutectic solvents of betaine-acetic acid, L-proline-acetic acid, glucose-acetic acid and choline chloride-acetic acid were measured with a rotational viscometer and were 4.90, 5.96, 8.52 and 6.28 mPa.s, respectively. In order to be extracted, the target compound must diffuse from the solid phase to the liquid phase. If the viscosity of the liquids is lower, their rate of diffusion will obviously be greater. That is, the greater the viscosity of the eutectic solvent, the poorer its ability to extract flavonoid compounds from polygonatum sibiricum. Meanwhile, when different molecular structures are used as hydrogen bond acceptors of the eutectic solvent, the properties of the solvent (viscosity, density, polarity, surface tension, and pH) may be affected, thereby adjusting the extraction effect. Compared with other three eutectic solvents, the eutectic solvent synthesized by betaine and acetic acid is probably more similar to most of flavonoids compounds in rhizoma polygonati in polarity, forms hydrogen bonds with most of flavonoids compounds in rhizoma polygonati, has stronger hydrophobic interaction, is closer to the pH value of most of flavonoids compounds in rhizoma polygonati, and is more beneficial to extraction of the flavonoids compounds in rhizoma polygonati. Therefore, betaine-acetic acid was chosen as the best extraction solvent for subsequent experiments.
2. Screening for optimum molar ratios
The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor affects the physicochemical properties of the eutectic solvent, and has a significant effect on the extraction rate. Different molar ratios (1: 1, 1:2, 1:3, 1:4, 1:5, 1: 6) of betaine and acetic acid were investigated in this experiment, and other extraction conditions were betaine-acetic acid, water content 30%, feed-to-liquid ratio 1:100g mL-1The extraction temperature is 50 ℃, and the extraction time is 30 min. The results in fig. 2 show that the extraction yield is highest at a 1:4 molar ratio of betaine to acetic acid. Therefore, the experiment selects the mol ratio of 1:4 to extract the flavonoid compounds in the rhizoma polygonati.
3. Screening for optimum moisture content
Experiments were performed at different water contents (20%, 30%, 40%, 60%, 80%). As shown in fig. 3, it can be seen that the extraction rate gradually increases, reaches a peak at a water content of 30%, and then gradually decreases as the water content increases. The initial increase in extraction yield may be due to the addition of water which reduces the viscosity of the eutectic solvent, which facilitates the extraction of flavonoids from polygonatum sibiricum. However, the addition of too much water may disrupt the hydrogen bonding interaction between the hydrogen bond acceptor and the hydrogen bond donor, reducing the extractability of the eutectic solvent. So in subsequent experiments we chose a moisture content of 30%.
4. Screening of optimum feed-to-liquid ratio
The experiment investigated the feed-to-liquid ratio (1: 10, 1:30, 1:50, 1:70, 1:90, 1:100, 1:120 g mL)-1) Influence on the extraction rate of flavonoid compounds in rhizoma Polygonati, and other extraction conditions comprise betaine-acetic acid with a molar ratio of 1:4, a water content of 30%, an extraction temperature of 50 deg.C, and an extraction time of 30 min. As can be seen from FIG. 4, the extraction rate increased first with increasing ratio, at 1:100 gmL-1The extraction rate is highest when the ratio is more than 1:100g mL-1The post extraction rate is slightly reduced. Therefore, the material-liquid ratio is selected to be 1:100 gmL-1Extracting flavonoid compounds from rhizoma Polygonati.
5. Screening for optimum extraction temperature
The experiment examines the influence of extraction temperature (20, 30, 40, 50, 60 ℃) on the extraction rate of the flavonoid compounds in the sealwort, and other extraction conditions are betaine-acetic acid with the molar ratio of 1:4, the water content of 30 percent and the feed-liquid ratio of 1:100g mL-1The extraction time is 30 min. As can be seen from fig. 5, the extraction rate gradually increased with the increase in temperature, and the extraction rate was the highest when the temperature reached 50 ℃ and decreased when the temperature exceeded 50 ℃. The reason may be that mass transfer is enhanced with the increase of extraction temperature, and molecular diffusion can be accelerated, so that more flavonoid compounds in polygonatum are extracted, but the structure of flavonoid compounds in part of polygonatum is damaged at higher temperature, and the biological activity of flavonoid compounds is influenced. In summary, we chose an extraction temperature of 50 ℃.
6. Screening for optimal extraction time
The experiment researches the influence of extraction time (10, 20, 30, 40, 60 min) on the extraction rate of flavonoid compounds in rhizoma Polygonati, and other extraction conditions are betaine-acetic acid with a molar ratio of 1:4, a water content of 30% and a feed-liquid ratio of 1:100g mL-1The extraction temperature was 50 ℃. As can be seen from the results of FIG. 6, the extraction rate was in an upward trend in the time range of 10-30min, and the extraction rate was in a downward trend as the extraction time was extended. The reason may be that the extraction rate of the flavonoid compounds in the polygonatum sibiricum can be improved within a certain time, but the structure of the flavonoid compounds in the polygonatum sibiricum can be damaged by too long extraction time, so that the extraction rate is reduced. Therefore, the extraction time is selected to be 30 min.
Example 2
The method comprises the following steps of (1) measuring the free radical scavenging capacity of eutectic solvent flavone extract and ethanol flavone extract with different extract contents, wherein the method comprises the following specific steps:
the capacity of eliminating DPPH free radicals and hydroxyl free radicals of eutectic solvent extract and ethanol extract with different extract contents is measured. Free radicals have certain functions, such as immunity and signaling processes. However, excessive free radicals have destructive actions, resulting in the destruction of normal cells and tissues of the human body, thereby causing various diseases such as heart disease, senile dementia, parkinson's disease and tumor. In addition, sunlight radiation, air pollution, smoking, pesticides and the like in the external environment can cause the human body to generate more free radicals, which are the root cause of human aging and diseases. Therefore, the removal of excessive free radicals in the body is of great significance for preventing various diseases of the human body.
The results of the activity of the extract in scavenging DPPH free radicals and hydroxyl free radicals show that (fig. 7 (a) (b)), the flavonoids compounds in the rhizoma polygonati have strong capability in scavenging DPPH free radicals and hydroxyl free radicals, and the activity of the eutectic solvent extract in scavenging DPPH free radicals and hydroxyl free radicals is better than that of the ethanol extract under the same extraction conditions. The reason is probably that the types and the contents of the flavonoid compounds of the sealwort in the eutectic solvent extracting solution have higher antioxidant activity than the flavonoid compounds in the ethanol extracting solution, and are more favorable for preventing various diseases of human bodies.
Example 3
The eutectic solvent is recycled, different carbon adsorption materials have different polarities, particle sizes, intermolecular forces and the like, and the adsorption effect on flavonoid compounds in the sealwort is different, so that an optimization experiment needs to be performed on the types of carbon materials by the adsorption of the flavonoid compounds in the sealwort, and the operation is as follows:
the adsorption behavior of the adsorbent material may not only be related to polarity, but also be influenced by the specific surface area and pore size. That is, when the polarity of the adsorbent is similar to that of the adsorbate, it exhibits a higher adsorption capacity. Meanwhile, the larger specific surface area and the larger pore diameter are beneficial to improving the adsorption performance. In the research, the recovery of the eutectic solvent is realized by adsorbing flavonoid compounds in the eutectic solvent extracting solution by adopting four different carbon adsorbing materials with small particle size, large specific surface area, good stability and large adsorption capacity, ultrahigh-capacitance porous carbon, graphene, multi-wall carbon nanotubes and carbon black, so that the solvent can be recycled. The results show that the four carbon adsorption materials have strong adsorption capacity (fig. 8), wherein the adsorption capacity of the ultrahigh-capacitance porous carbon is the strongest and can reach 94%. The reason may be that the ultrahigh-capacitance porous carbon has a large specific surface area, a small particle size, a polarity similar to that of flavonoids in polygonatum, and van der Waals interaction force between molecules of the ultrahigh-capacitance porous carbon and the flavonoids in polygonatum, and is good in stability, large in adsorption capacity and strong in adsorption capacity.
The recovered eutectic solvent was used for a new repeated extraction (fig. 9), and after ten times of the cycle extraction, it was found that the extraction rate was not decreased, and the extraction rate was 93% in the tenth cycle extraction, indicating that the recovered eutectic solvent could be reused more than ten times. Therefore, the selected eutectic solvent is not only green, but also has good recoverability and can be repeatedly used.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art can change or modify the technical content disclosed above into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (10)
1. A method for green and high-efficiency extraction of flavonoid compounds in rhizoma polygonati by using a eutectic solvent is characterized by comprising the following steps: the method comprises the following steps:
firstly, preparing a eutectic solvent as an extracting agent, taking any one of betaine, L-proline, glucose and choline chloride as a hydrogen bond receptor, taking acetic acid as a hydrogen bond donor, mixing the materials in proportion, and heating and stirring the mixture at 80 ℃ until the mixture becomes uniform, transparent and clear liquid to form the eutectic solvent;
secondly, crushing a rhizoma polygonati sample to obtain rhizoma polygonati powder, weighing the rhizoma polygonati powder in a test tube, adding a prepared eutectic solvent, carrying out ultrasonic extraction, then centrifuging to obtain a flavonoid compound extracting solution in the rhizoma polygonati, establishing a rutin standard curve by taking rutin as a standard substance, and measuring the extraction rate of flavonoid compounds in the rhizoma polygonati;
and thirdly, evaluating the antioxidant activity of the flavonoid compounds in the extracting solution, adsorbing the flavonoid compounds in the extracting solution by adopting a carbon adsorbing material, and performing vortex, centrifugation and filtration to realize the recovery and reutilization of the eutectic solvent of the extracting agent.
2. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: in the first step, the hydrogen bond acceptor is betaine, and the molar ratio of the betaine to acetic acid is 1: 4.
3. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: in the second step, the grain size of the rhizoma polygonati powder is 0.425 mm.
4. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: in the second step, the ratio of the sealwort powder to the eutectic solvent is 1:100 g/mL.
5. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: in the second step, the ultrasonic extraction temperature is 50 ℃, and the extraction time is 30 min.
6. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: the rotation speed of the centrifugation in the second step is 4000 rpm, and the centrifugation time is 5 min.
7. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: and thirdly, evaluating the antioxidant activity of the flavonoid compounds in the extracting solution, including measuring the DPPH free radical and hydroxyl free radical scavenging capacity.
8. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: in the third step, the carbon adsorption material is 100mg of ultrahigh-capacitance porous carbon.
9. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: and in the third step, the vortex time is 10 min, the centrifugal rotation speed is 4000 rpm, the centrifugal time is 10 min, and a membrane filter with the pore diameter of 0.22 mu m is adopted for filtration.
10. The method for green and efficient extraction of flavonoids from polygonatum sibiricum by using the eutectic solvent according to claim 1, wherein the method comprises the following steps: the extracted sample rhizoma polygonati is a plant with homology of medicine and food.
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| CN115154555A (en) * | 2022-07-13 | 2022-10-11 | 山西农业大学 | Green and sustainable millet polyphenol extraction method and application |
| CN115531490A (en) * | 2022-10-17 | 2022-12-30 | 浙江工业大学 | Method for quickly separating eutectic solvent from dendrobium officinale flavone extracting solution |
| CN115531490B (en) * | 2022-10-17 | 2023-09-01 | 浙江工业大学 | Method for rapidly separating eutectic solvent from dendrobium candidum flavone extract |
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| CN116350722A (en) * | 2023-04-10 | 2023-06-30 | 河北科技师范学院 | Method for extracting flavone from asparagus by using eutectic solvent |
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