CN111214612A - Green extraction method of flavonoid components in polygonatum odoratum - Google Patents
Green extraction method of flavonoid components in polygonatum odoratum Download PDFInfo
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- CN111214612A CN111214612A CN201811414779.6A CN201811414779A CN111214612A CN 111214612 A CN111214612 A CN 111214612A CN 201811414779 A CN201811414779 A CN 201811414779A CN 111214612 A CN111214612 A CN 111214612A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a green extraction method of flavonoid components in polygonatum odoratum, which comprises the following steps: drying a polygonatum sample, crushing and sieving the polygonatum sample by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 65-75 ℃ until uniform liquid is formed to obtain NADESs; placing rhizoma Polygonati Odorati powder in round bottom flask, adding NADESs, mixing, soaking, placing in constant temperature water bath, ultrasonic extracting for 2-4 times (each time for 30-40 min), cooling to room temperature, transferring the solution into a centrifuge tube, centrifuging, removing precipitate to obtain supernatant, and concentrating under reduced pressure. The NADESs are used as an extraction medium, so that the method has the advantages of high efficiency, environmental protection, reusability and the like, can comprehensively and effectively extract the flavonoid compounds in the polygonatum odoratum, has the activities of oxidation resistance, aging resistance and tumor resistance, and can be prepared in a large scale.
Description
Technical Field
The invention relates to a green extraction method of flavonoid components in polygonatum odoratum.
Background
Rhizoma Polygonati Odorati is Liliaceae plant rhizoma Polygonati OdoratiPolygonatum odoratumDried rhizome of Druce contains various active substances such as flavone, polysaccharide, alkaloid, etc. Wherein the flavonoid is yellow pigment generated by photosynthesis, and has effects of enhancing immunity, resisting oxidation, resisting aging, and resisting tumor. Therefore, the method for extracting flavone from polygonatum odoratum has important significance in the fields of food and medicines. At present, the flavonoid components of the traditional Chinese medicine mainly take an organic solvent as an extraction medium, and have the problems of low extraction efficiency, high cost investment, serious organic solvent pollution and the like. Therefore, the search of novel extraction solvents for extracting flavonoid components in traditional Chinese medicines is highly valued.
The development of green solvents has prompted the development of modern green chemistry that achieves high utilization and low toxicity by replacing volatile solvents. Deep Eutectic Solvents (DESs) are considered as a novel green solvent due to their excellent physicochemical properties and low synthesis cost, and have been used in the fields of electrochemistry, nanomaterials and the like. For the advantages of DESs, researchers have applied them to the fields of food, medicine, etc., as a green extraction medium for extracting active ingredients such as phenolic acids, alkaloids, polysaccharides, etc. Research on flavonoid components has been reported abroad that researchers have successfully extracted flavonoid components from traditional Chinese medicines such as epimedium, sophora japonica, ginkgo biloba leaves and the like by utilizing DESs. With the progress of research, natural deep eutectic solvents (nadees) further reduce toxicity on the basis of the ees, and become a research hotspot. Nadees are mainly composed of cellular primary metabolites, and by heating, Hydrogen Bond Acceptors (HBAs) and Hydrogen Bond Donors (HBDs) of solid halide salts combine to form a eutectic mixture. At present, researchers have successfully applied the NADESs to the extraction of traditional Chinese medicine flavonoids such as medlar and dalbergia wood, but compared with the traditional organic solvent and DESS, the research on the extraction of traditional Chinese medicine flavonoids by NADESs is still limited, and domestic reports are few.
Disclosure of Invention
The invention aims to provide a green extraction method of flavonoid components in polygonatum odoratum
The invention is realized by the following technical scheme:
a green extraction method of flavonoid components in polygonatum odoratum comprises the following steps: drying a polygonatum sample at 35-45 ℃ to constant mass, crushing and sieving by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 65-75 ℃ until uniform liquid is formed to obtain NADESs; placing 10-20 parts of radix polygonati officinalis powder into a round-bottom flask, adding 75-85 parts of NADESs, uniformly mixing, soaking for 23-25h, placing into a constant-temperature water bath, performing ultrasonic extraction at 45-55 ℃ for 2-4 times, extracting for 30-40min each time, cooling to room temperature, transferring the solution into a centrifugal tube, centrifuging at 2500r/min for 25-35min, removing the precipitate to obtain a supernatant, and concentrating under reduced pressure to obtain the polygonatum odoratum powder; the raw materials are in parts by weight.
Preferably, in the extraction method, the polygonatum sample is dried at 40 ℃ to a constant mass.
Preferably, in the extraction method, the heating and stirring are carried out at 70 ℃.
Preferably, in the extraction method, the soaking is carried out for 24 hours.
Preferably, in the extraction method, the ultrasonic extraction is carried out for 3 times at 50 ℃.
Preferably, in the extraction method, each extraction time is 35 min.
Preferably, in the extraction method, centrifugation is carried out for 30min at 2500 r/min.
The invention has the technical effects that:
the NADESs are used as an extraction medium, so that the method has the advantages of high efficiency, environmental protection, reusability and the like, can comprehensively and effectively extract the flavonoid compounds in the polygonatum odoratum, has the activities of oxidation resistance, aging resistance and tumor resistance, and can be prepared in a large scale.
Detailed Description
The following describes the substance of the present invention with reference to the examples.
Example 1
A green extraction method of flavonoid components in polygonatum odoratum comprises the following steps: drying a polygonatum sample at 40 ℃ to constant mass, crushing and sieving by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 70 ℃ until uniform liquid is formed to obtain NADESs; placing 15 parts of radix polygonati officinalis powder in a round-bottom flask, adding 80 parts of NADESs, uniformly mixing, soaking for 24h, placing in a constant-temperature water bath kettle, performing ultrasonic extraction at 50 ℃ for 3 times, extracting for 35min each time, cooling to room temperature, transferring the solution into a centrifugal tube, centrifuging at 2500r/min for 30min, removing precipitate to obtain a supernatant, and concentrating under reduced pressure to obtain the final product; the raw materials are in parts by weight.
Example 2
A green extraction method of flavonoid components in polygonatum odoratum comprises the following steps: drying a polygonatum sample at 35 ℃ to constant mass, crushing and sieving by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 65 ℃ until uniform liquid is formed to obtain NADESs; placing 10 parts of rhizoma Polygonati Odorati powder in a round-bottom flask, adding 75 parts of NADESs, mixing, soaking for 23h, placing in a constant temperature water bath, performing ultrasonic extraction at 45 deg.C for 2 times, each time for 30min, cooling to room temperature, transferring the solution into a centrifugal tube, centrifuging at 2500r/min for 25min, removing precipitate to obtain supernatant, and concentrating under reduced pressure; the raw materials are in parts by weight.
Example 3
A green extraction method of flavonoid components in polygonatum odoratum comprises the following steps: drying a polygonatum sample at 45 ℃ to constant mass, crushing and sieving by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 75 ℃ until uniform liquid is formed to obtain NADESs; placing 20 parts of rhizoma Polygonati Odorati powder in a round-bottom flask, adding 85 parts of NADESs, mixing, soaking for 25 hr, placing in a constant temperature water bath, performing ultrasonic extraction at 55 deg.C for 4 times, each time for 40min, cooling to room temperature, transferring the solution into a centrifuge tube, centrifuging at 2500r/min for 35min, removing precipitate to obtain supernatant, and concentrating under reduced pressure; the raw materials are in parts by weight.
The NADESs are used as an extraction medium, so that the method has the advantages of high efficiency, environmental protection, reusability and the like, can comprehensively and effectively extract the flavonoid compounds in the polygonatum odoratum, has the activities of oxidation resistance, aging resistance and tumor resistance, and can be prepared in a large scale.
Claims (7)
1. A green extraction method of flavonoid components in polygonatum odoratum is characterized by comprising the following steps: drying a polygonatum sample at 35-45 ℃ to constant mass, crushing and sieving by a 80-mesh sieve; uniformly mixing sodium acetate, lactic acid and distilled water according to the mass ratio of 1:3:7, heating and stirring at 65-75 ℃ until uniform liquid is formed to obtain NADESs; placing 10-20 parts of radix polygonati officinalis powder into a round-bottom flask, adding 75-85 parts of NADESs, uniformly mixing, soaking for 23-25h, placing into a constant-temperature water bath, performing ultrasonic extraction at 45-55 ℃ for 2-4 times, extracting for 30-40min each time, cooling to room temperature, transferring the solution into a centrifugal tube, centrifuging at 2500r/min for 25-35min, removing the precipitate to obtain a supernatant, and concentrating under reduced pressure to obtain the polygonatum odoratum powder; the raw materials are in parts by weight.
2. The extraction method according to claim 1, characterized in that: drying the polygonatum sample at 40 ℃ to constant mass.
3. The extraction method according to claim 1, characterized in that: heated and stirred at 70 ℃.
4. The extraction method according to claim 1, characterized in that: soaking for 24 h.
5. The extraction method according to claim 1, characterized in that: extracting with ultrasound at 50 deg.C for 3 times.
6. The extraction method according to claim 5, characterized in that: extracting for 35min each time.
7. The extraction method according to claim 1, characterized in that: centrifuging at 2500r/min for 30 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811414779.6A CN111214612A (en) | 2018-11-26 | 2018-11-26 | Green extraction method of flavonoid components in polygonatum odoratum |
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| CN201811414779.6A CN111214612A (en) | 2018-11-26 | 2018-11-26 | Green extraction method of flavonoid components in polygonatum odoratum |
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| CN111214612A true CN111214612A (en) | 2020-06-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114377428A (en) * | 2021-12-23 | 2022-04-22 | 太谷养膳大健康食业有限公司 | Method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by applying eutectic solvent |
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- 2018-11-26 CN CN201811414779.6A patent/CN111214612A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114377428A (en) * | 2021-12-23 | 2022-04-22 | 太谷养膳大健康食业有限公司 | Method for green and efficient extraction of flavonoid compounds in rhizoma polygonati by applying eutectic solvent |
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