CN114364785A - 对数字微流控设备的温度控制 - Google Patents

对数字微流控设备的温度控制 Download PDF

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Publication number
CN114364785A
CN114364785A CN202080061054.2A CN202080061054A CN114364785A CN 114364785 A CN114364785 A CN 114364785A CN 202080061054 A CN202080061054 A CN 202080061054A CN 114364785 A CN114364785 A CN 114364785A
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China
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substrate
heating element
microfluidic device
temperature
substrate structure
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Pending
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CN202080061054.2A
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English (en)
Chinese (zh)
Inventor
林彦佑
龚剑
弗兰克·钟
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Shenzhen Mgi Holdings Co ltd
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BGI Shenzhen Co Ltd
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Publication of CN114364785A publication Critical patent/CN114364785A/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K1/00—Details of thermometers not specially adapted for particular types of thermometer
    • G01K1/02—Means for indicating or recording specially adapted for thermometers
    • G01K1/026—Means for indicating or recording specially adapted for thermometers arrangements for monitoring a plurality of temperatures, e.g. by multiplexing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B2203/00—Integrated processes for the production of hydrogen or synthesis gas
    • C01B2203/16—Controlling the process
    • C01B2203/1614—Controlling the temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K2217/00—Temperature measurement using electric or magnetic components already present in the system to be measured

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080061054.2A 2019-08-28 2020-08-27 对数字微流控设备的温度控制 Pending CN114364785A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962893091P 2019-08-28 2019-08-28
US62/893,091 2019-08-28
PCT/CN2020/111570 WO2021037094A1 (en) 2019-08-28 2020-08-27 Temperature control on digital microfluidics device

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Publication Number Publication Date
CN114364785A true CN114364785A (zh) 2022-04-15

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US (3) US11660602B2 (https=)
EP (1) EP4022031A4 (https=)
JP (1) JP7595648B2 (https=)
CN (1) CN114364785A (https=)
WO (1) WO2021037094A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117742410A (zh) * 2023-12-26 2024-03-22 首都医科大学附属北京儿童医院 一种温控系统及微流控芯片检测系统

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114364785A (zh) 2019-08-28 2022-04-15 深圳华大基因科技有限公司 对数字微流控设备的温度控制
CN112791753B (zh) * 2019-11-13 2022-05-24 京东方科技集团股份有限公司 微流控芯片及其制造方法、微流控器件
CN113751089A (zh) * 2021-09-02 2021-12-07 厦门大学 一种集成有加热模块的数字微流控芯片
CN114100716B (zh) * 2021-12-01 2023-04-21 上海天马微电子有限公司 微流控装置及其驱动方法
TWI810990B (zh) * 2022-06-27 2023-08-01 友達光電股份有限公司 微流體晶片及其使用方法
CN116731841A (zh) * 2022-11-10 2023-09-12 南方科技大学 微针加热扩增结构、快速核酸检测芯片、装置与方法

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WO2009003184A1 (en) * 2007-06-27 2008-12-31 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
CN104248997A (zh) * 2013-06-27 2014-12-31 李木 一种数字式微流控芯片及其控制方法
CA2846963A1 (en) * 2014-03-14 2015-09-14 Karan Kaler Droplet-based microfluidic device and methods for producing and using the same
CN107532128A (zh) * 2015-04-24 2018-01-02 豪夫迈·罗氏有限公司 使用数字微流体的数字pcr系统和方法

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US8735103B2 (en) * 2006-12-05 2014-05-27 Electronics And Telecommunications Research Institute Natural convection-driven PCR apparatus and method using disposable polymer chip
WO2011066185A1 (en) * 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8940147B1 (en) * 2011-04-25 2015-01-27 Sandia Corporation Microfluidic hubs, systems, and methods for interface fluidic modules
US9797007B2 (en) * 2012-11-30 2017-10-24 Suzhou Institute Of Nano-Tech And Nano-Bionics Of Chinese Academy Of Science Micro-liquid phase reaction method based on substrate with hydrophilic-hydrophobic patterned surface
KR20160081896A (ko) * 2013-08-30 2016-07-08 일루미나, 인코포레이티드 친수성 또는 다양한-친수성 표면 상에서의 액적의 조작
WO2016197106A1 (en) * 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
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WO2018138993A1 (ja) * 2017-01-30 2018-08-02 株式会社村田製作所 温度センサ
EP3615219A4 (en) * 2017-04-26 2021-04-28 Berkeley Lights, Inc. Biological process systems and methods using microfluidic apparatus having an optimized electrowetting surface
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WO2009003184A1 (en) * 2007-06-27 2008-12-31 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
CN101679932A (zh) * 2007-06-27 2010-03-24 数字化生物系统 用于热交换化学过程的基于数字微流体的装置
CN104248997A (zh) * 2013-06-27 2014-12-31 李木 一种数字式微流控芯片及其控制方法
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117742410A (zh) * 2023-12-26 2024-03-22 首都医科大学附属北京儿童医院 一种温控系统及微流控芯片检测系统
CN117742410B (zh) * 2023-12-26 2024-11-15 首都医科大学附属北京儿童医院 一种温控系统及微流控芯片检测系统

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US20230249186A1 (en) 2023-08-10
US11980885B2 (en) 2024-05-14
EP4022031A1 (en) 2022-07-06
US11660602B2 (en) 2023-05-30
EP4022031A4 (en) 2023-09-27
US20240253046A1 (en) 2024-08-01
WO2021037094A1 (en) 2021-03-04
JP2022547439A (ja) 2022-11-14
JP7595648B2 (ja) 2024-12-06
US20210114034A1 (en) 2021-04-22
US12269038B2 (en) 2025-04-08

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