CN114350513A - Cell subculture batch operation device and method - Google Patents
Cell subculture batch operation device and method Download PDFInfo
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- CN114350513A CN114350513A CN202111626707.XA CN202111626707A CN114350513A CN 114350513 A CN114350513 A CN 114350513A CN 202111626707 A CN202111626707 A CN 202111626707A CN 114350513 A CN114350513 A CN 114350513A
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Abstract
The invention relates to a cell subculture batch operation device and a cell subculture batch operation method, wherein a culture dish is placed in a placing groove by opening a switch door, then the switch door is closed to enable the interior of a box body to form a sealed state, the temperature in the box body is detected by a temperature detector, so that the temperature in the box body is monitored in real time according to the temperature detector, when the temperature is too low, an air pump II can be opened to draw external air into a heater, then the air is heated and sprayed out through a nozzle, the temperature in the box body is kept at a set temperature, when the temperature in the box body is too high, the air pump I is started to draw the air into a semiconductor cooler, so that the constant temperature in the box body is kept, and meanwhile, a motor is started to drive a rotating rod to drive fan blades to rotate.
Description
Technical Field
The invention relates to the technical field of cell batch culture, in particular to an operating device and an operating method for cell subculture batch culture.
Background
Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. A large number of cells or their metabolites are obtained by cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology.
When the existing cell culture device is used, most of the culture devices do not have a constant temperature effect, so that the cell culture cannot grow in the culture device for a long time, the use efficiency is greatly reduced, in addition, even if constant temperature equipment is designed, because air circulation does not exist, many parts of the space cannot be fully heated uniformly, and then vibration is large in the transportation process, so that a cell passage batch culture operation device and a cell passage batch culture operation method are provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an operating device and a method for cell subculture batch culture, wherein a culture dish is placed in a placing groove by opening a switch door, then the switch door is closed to enable the interior of a box body to form a sealed state, then the temperature inside the box body is detected by a temperature detector, so that the temperature inside the box body is monitored in real time according to the temperature detector, when the temperature is too low, a second air pump can be opened to pump external air into a heater, then the air is heated and sprayed out through a nozzle, the temperature inside the box body is kept at a set temperature, when the temperature inside the box body is too high, a first air pump is started to pump the air into a semiconductor cooler, so that the constant temperature is kept in the box body, meanwhile, a motor is started to drive a rotating rod to drive fan blades to rotate, so that the air inside the box body continuously circulates and flows, and can pass through dry filter screen at the in-process that flows, moisture in the air behind the dry filter screen can constantly absorb to guarantee the degree of drying in the box, remove through the universal wheel when needs box removes, and cushion through the cooperation of last sliding barrel, outer sliding barrel and buffer spring, cushion the spacing of culture dish also through side buffering sponge and bottom buffering sponge simultaneously.
In order to achieve the purpose, the invention provides the following technical scheme: an operation device and method for cell subculture in batch comprises a box body, wherein a controller is fixedly arranged on the outer surface of the box body, a switch door is movably arranged on the outer surface of the box body, a circulation box is fixedly arranged on the outer surface of the right side of the box body, a circulation structure is fixedly arranged at the top of the circulation box, a first air pump is fixedly arranged at the top of the box body, a transmission pipe extending into the box body is fixedly arranged on the outer surface of the right side of the first air pump, the circulation structure comprises a motor positioned at the top of the circulation box, two drying filter screens are fixedly arranged in the circulation box, a second air pump is fixedly arranged on the inner bottom wall of the box body, a partition plate is fixedly arranged in the box body, a heater is fixedly arranged on the outer surface of the partition plate, a gas pipe is fixedly arranged at the bottom of the heater, two support plates are fixedly arranged in the box body, two the equal fixed mounting in top of backup pad has the cultivation to place the frame, the interior roof fixed mounting of box has the semiconductor cooler, the output shaft department fixed mounting of motor has the bull stick that extends to the circulation incasement portion, the fixed surface mounting of bull stick has quantity to be three flabellum.
A plurality of groups of buffer placing cylinder assemblies are arranged at the inner bottom of the culture placing frame at equal intervals, and culture dishes are placed on the inner sides of the buffer placing cylinder assemblies;
the bottom of box is equidistant to be equipped with and removes buffer section of thick bamboo subassembly, and the base is installed to the bottom of this removal buffer section of thick bamboo subassembly, the universal wheel is installed to the bottom four corners department of base.
Preferably, the top of circulation case is seted up with the through hole of bull stick surface looks adaptation, the left side surface of circulation case is seted up quantity and is two and the communicating vent of box inside.
Preferably, two the ventilation hole that quantity is six is all seted up to the inside of backup pad, two the frame is placed in the cultivation is the spill.
Preferably, the inside fixed mounting in left side of box has the quantity to be two light, the gas-supply pipe is connected with the right side of No. two air pumps with heater bottom looks detached one end.
Preferably, the top of the semiconductor cooler is connected with the delivery pipe, and the central point of the box body and the central point of the support plate are on the same vertical line.
Preferably, a temperature detector is fixedly mounted on the inner wall of the right side of the box body, and at least ten nozzles are fixedly mounted at the top of the heater and the bottom of the semiconductor cooler.
Preferably, the buffer placing cylinder assembly comprises a placing groove, a bottom buffer sponge and a side buffer sponge, wherein the placing groove is arranged at the inner bottom, the bottom buffer sponge is arranged at the bottom of the inner wall of the placing groove, the side buffer sponge is arranged on two sides of the inner wall of the placing groove, and a culture dish is clamped on the inner side of the side buffer sponge and the top of the bottom buffer sponge.
Preferably, the movable buffer cylinder component comprises an upper sliding cylinder, a limiting block, a buffer spring and an outer sliding cylinder, the upper sliding cylinder is installed at the bottom of the box body, the buffer spring is installed at the inner top of the upper sliding cylinder, the outer sliding cylinder is installed at the bottom of the buffer spring, the outer sliding cylinder is sleeved on the outer side, and the limiting block is installed at the position, close to the outer sliding cylinder, of the top of the base.
A cell subculture batch operation method comprises the following steps:
step 1: firstly, placing the culture dish in the placing groove by opening the switch door, and then closing the switch door to enable the interior of the box body to form a sealed state;
step 2: then the temperature inside the box body is detected through the temperature detector, so that the temperature inside the box body is monitored in real time according to the temperature detector;
and step 3: when the temperature is too low, the second air pump can be opened to pump external air into the heater, and then the air is heated and sprayed out through the nozzle, so that the temperature in the box body is kept at the set temperature;
and 4, step 4: when the temperature in the box body is too high, air is pumped into the semiconductor cooler by starting the first air pump, so that the constant temperature in the box body is kept;
and 5: meanwhile, the motor is started to drive the rotating rod to drive the fan blades to rotate, so that air in the box body continuously and circularly flows;
step 6: the water in the air passing through the drying filter screen can be continuously absorbed by the drying filter screen in the flowing process, so that the drying degree in the box body is ensured;
and 7: when the box body needs to move, the box body moves through the universal wheels and is buffered through the matching of the upper sliding barrel, the outer sliding barrel and the buffer spring;
and 8: simultaneously, the limit of the culture dish is also buffered by the side buffer sponge and the bottom buffer sponge.
Compared with the prior art, the technical scheme of the application has the following beneficial effects:
firstly, the culture dish is placed in the placing groove by opening the switch door, then the switch door is closed to enable the interior of the box body to form a sealing state, then the temperature inside the box body is detected by the temperature detector, thereby the temperature inside the box body is monitored in real time according to the temperature detector, when the temperature is too low, the second air pump can be opened to pump the external air into the heater, then the air is heated and sprayed out by the nozzle, the temperature inside the box body is kept at the set temperature, when the temperature inside the box body is too high, the first air pump is started to pump the air into the semiconductor cooler, so that the constant temperature is kept in the box body, meanwhile, the motor is started to drive the rotating rod to drive the fan blades to rotate, the air inside the box body continuously and circularly flows, and passes through the drying filter screen in the flowing process, and the moisture in the air passing through the drying filter screen can be continuously absorbed, thereby guarantee the dry degree in the box, remove through the universal wheel when needs the box to remove to cushion through the cooperation of last slide cartridge, outer slide cartridge and buffer spring, cushion the spacing of culture dish also through side buffering sponge and bottom buffering sponge simultaneously.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of a circulation structure of the present invention;
FIG. 3 is an enlarged view of the circulation structure of the present invention at a;
FIG. 4 is a schematic view of the assembly of the culture dish holder of the present invention;
FIG. 5 is an enlarged view of the structure of the present invention at b.
In the figure: the device comprises a box body 1, a controller 2, a door 3, a circulation box 4, a circulation structure 5, a motor 501, a drying filter screen 502, an air pump 503 II, a partition 504, a heater 505, air pipes 506, a supporting plate 507, a cultivation placing frame 508, a semiconductor cooler 509, a rotating rod 510, fan blades 511, a lighting lamp 512, a temperature detector 513, a nozzle 514, a vent 515, a ventilation opening 516, a ventilation opening 517, an air pump 6I, a transmission pipe 7, a base 8, a culture dish 9, a buffer sponge 10 on the side, a placing groove 11, a buffer sponge 12 on the bottom, a sliding barrel 13, a limiting block 14, a buffer spring 15, an outer sliding barrel 16 and a universal wheel 18.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the cell subculture batch operation device in this embodiment includes a box body 1, a controller 2 is fixedly installed on an outer surface of the box body 1, a switch door 3 is movably installed on an outer surface of the box body 1, a circulation box 4 is fixedly installed on an outer surface of a right side of the box body 1, a circulation structure 5 is fixedly installed on a top of the circulation box 4, a first air pump 6 is fixedly installed on the top of the box body 1, a delivery pipe 7 extending into the box body 1 is fixedly installed on an outer surface of a right side of the first air pump 6, the circulation structure 5 includes a motor 501 located on the top of the circulation box 4, two drying screens 502 are fixedly installed in the circulation box 4, a second air pump 503 is fixedly installed on an inner bottom wall of the box body 1, a partition 504 is fixedly installed in the box body 1, a heater 505 is fixedly installed on an outer surface of the partition 504, and an air pipe 506 is fixedly installed at a bottom of the heater 505, two support plates 507 are fixedly arranged in the box body 1, culture placing frames 508 are fixedly arranged at the tops of the two support plates 507, a semiconductor cooler 509 is fixedly arranged on the inner top wall of the box body 1, a rotating rod 510 extending into the circulating box 4 is fixedly arranged at an output shaft of the motor 501, three fan blades 511 are fixedly arranged on the outer surface of the rotating rod 510, a plurality of groups of buffer placing cylinder assemblies are arranged at equal intervals at the inner bottom of the culture placing frames 508, and culture dishes 9 are placed on the inner sides of the buffer placing cylinder assemblies;
the bottom of box 1 is equidistant to be equipped with and removes buffer section of thick bamboo subassembly, and base 8 is installed to this bottom of removing buffer section of thick bamboo subassembly, and universal wheel 18 is installed to the bottom four corners department of base 8.
Firstly, the culture dish is placed in the placing groove 11 by opening the switch door 3, then the switch door 3 is closed to enable the interior of the box body 1 to form a sealing state, then the temperature inside the box body 1 is detected by the temperature detector 513, the temperature inside the box body 1 is monitored in real time according to the temperature detector 513, when the temperature is too low, the second air pump 503 can be opened to pump the external air into the heater 505, then the air is heated and sprayed out through the nozzle 514, the temperature inside the box body 1 is kept at the set temperature, when the temperature inside the box body 1 is too high, the first air pump 6 is started to pump the air into the semiconductor cooler 6, so that the constant temperature is kept in the box body 1, meanwhile, the motor 501 is started to drive the rotating rod 510 to drive the fan blades 511 to rotate, and the air inside the box body 1 continuously and circularly flows, and can pass through dry filter screen 502 at the in-process that flows, moisture in the air behind dry filter screen 502 can constantly absorb to guarantee the dry degree in the box 1, remove through universal wheel 18 when needing box 1 to remove, and cushion through the cooperation of last slide cartridge 13, outer slide cartridge 16 and buffer spring 15, cushion through the spacing of side buffering sponge 10 and bottom buffering sponge 12 to culture dish 9 simultaneously also.
In this embodiment, the top of the circulation box 4 is provided with a through hole 517 matched with the outer surface of the rotating rod 510, and the outer surface of the left side of the circulation box 4 is provided with two ventilation holes 516 communicated with the inside of the box body 1.
In this embodiment, the inside of the two supporting plates 507 are opened with six ventilation holes 515, and the two cultivation placing frames 508 are both concave.
In this embodiment, two illuminating lamps 512 are fixedly installed inside the left side of the box 1, and the end of the air pipe 506 away from the bottom of the heater 505 is connected to the right side of the second air pump 503.
In this embodiment, the top of the semiconductor cooler 509 is connected to the duct 7, and the center point of the case 1 and the center point of the support plate 507 are on the same vertical line.
In this embodiment, a temperature detector 513 is fixedly installed on the right inner wall of the case 1, and not less than ten nozzles 514 are fixedly installed on the top of the heater 505 and the bottom of the semiconductor cooler 509.
In this embodiment, the buffer placing cylinder assembly includes a placing groove 11, a bottom buffer sponge 12 and a side buffer sponge 10, the placing groove 11 is installed at the inner bottom of 508, the bottom of the inner wall of the placing groove 11 is provided with the bottom buffer sponge 12, the two sides of the inner wall of the placing groove 11 are provided with the side buffer sponge 10, and the culture dish 9 is clamped at the inner side of the side buffer sponge 10 and the top of the bottom buffer sponge 12.
In this embodiment, the movable buffer cylinder assembly includes an upper sliding cylinder 13, a limiting block 14, a buffer spring 15 and an outer sliding cylinder 16, the upper sliding cylinder 13 is installed at the bottom of the box body 1, the buffer spring 15 is installed at the inner top of the upper sliding cylinder 13, the outer sliding cylinder 16 is installed at the bottom of the buffer spring 15, the outer sliding cylinder 16 is sleeved on the outer side of the upper sliding cylinder 13, and the limiting block 14 is installed at the position, close to the outer sliding cylinder 16, of the top of the base 8.
A cell subculture batch operation method comprises the following steps:
step 1: firstly, placing the culture dish in the placing groove 11 by opening the switch door 3, and then closing the switch door 3 to enable the interior of the box body 1 to form a sealed state;
step 2: then, the temperature inside the cabinet 1 is detected by the temperature detector 513, so that the temperature inside the cabinet 1 is monitored in real time according to the temperature detector 513;
and step 3: when the temperature is too low, the second air pump 503 can be turned on to draw the external air into the heater 505, and then the air is heated and sprayed out through the nozzle 514, so that the temperature inside the box body 1 is kept at the set temperature;
and 4, step 4: when the temperature in the box body 1 is too high, the air pump 6 is started to pump air into the semiconductor cooler 6, so that the constant temperature in the box body 1 is kept;
and 5: meanwhile, the motor 501 is started to drive the rotating rod 510 to drive the fan blades 511 to rotate, so that the air in the box body 1 continuously and circularly flows;
step 6: the air passes through the drying filter screen 502 in the flowing process, and the moisture in the air passing through the drying filter screen 502 is continuously absorbed, so that the drying degree in the box body 1 is ensured;
and 7: when the box body 1 needs to move, the box body is moved through the universal wheels 18, and buffering is performed through the matching of the upper sliding barrel 13, the outer sliding barrel 16 and the buffering spring 15;
and 8: meanwhile, the limit of the culture dish 9 is buffered by the side buffer sponge 10 and the bottom buffer sponge 12.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The utility model provides a cell subculture batch culture operating means, includes box (1), its characterized in that: the outer surface of the box body (1) is fixedly provided with a controller (2), the outer surface of the box body (1) is movably provided with a switch door (3), the outer surface of the right side of the box body (1) is fixedly provided with a circulating box (4), the top of the circulating box (4) is fixedly provided with a circulating structure (5), the top of the box body (1) is fixedly provided with a first air pump (6), and the outer surface of the right side of the first air pump (6) is fixedly provided with a transmission pipe (7) extending into the box body (1);
the circulating structure (5) comprises a motor (501) positioned at the top of a circulating box (4), the inside fixed mounting of the circulating box (4) is provided with two drying filter screens (502), the inner bottom wall of the box body (1) is fixedly provided with two air pumps (503), the inside fixed mounting of the box body (1) is provided with a partition board (504), the outer surface fixed mounting of the partition board (504) is provided with a heater (505), the bottom fixed mounting of the heater (505) is provided with an air pipe (506), the inside fixed mounting of the box body (1) is provided with two support plates (507), the top parts of the two support plates (507) are fixedly provided with a culture placement frame (508), the inner top wall fixed mounting of the box body (1) is provided with a semiconductor cooler (509), the output shaft of the motor (501) is fixedly provided with a rotating rod (510) extending to the inside of the circulating box (4), the outer surface of the rotating rod (510) is fixedly provided with three fan blades (511);
a plurality of groups of buffer placing cylinder assemblies are arranged at the inner bottom of the culture placing frame (508) at equal intervals, and culture dishes (9) are placed on the inner sides of the buffer placing cylinder assemblies;
the bottom of box (1) is equidistant to be equipped with and removes buffer cylinder subassembly, and base (8) are installed to the bottom of this removal buffer cylinder subassembly, universal wheel (18) are installed to the bottom four corners department of base (8).
2. A cell subculture batch operation apparatus according to claim 1, wherein: the top of circulation case (4) is seted up with through-hole (517) of bull stick (510) surface looks adaptation, the left side surface of circulation case (4) is seted up quantity and is two and with the inside communicating vent (516) of box (1).
3. A cell subculture batch operation apparatus according to claim 2, wherein: two ventilation hole (515) that quantity is six are all seted up to the inside of backup pad (507), two frame (508) are placed in the cultivation is the spill.
4. A cell subculture batch operation apparatus according to claim 3, wherein: the left side of the box body (1) is internally and fixedly provided with two illuminating lamps (512), and the end, away from the bottom of the heater (505), of the air pipe (506) is connected with the right side of the second air pump (503).
5. A cell subculture batch operation device according to claim 4, wherein: the top of the semiconductor cooler (509) is connected with the conveying pipe (7), and the central point of the box body (1) and the central point of the supporting plate (507) are on the same vertical line.
6. A cell subculture batch operation device according to claim 5, wherein: the temperature detector (513) is fixedly mounted on the inner wall of the right side of the box body (1), and the nozzles (514) with the number not less than ten are fixedly mounted at the top of the heater (505) and the bottom of the semiconductor cooler (509).
7. A cell subculture batch operation device according to claim 6, wherein: the buffer placing cylinder assembly comprises a placing groove (11), bottom buffer sponges (12) and side buffer sponges (10), the placing groove (11) is installed at the inner bottom of the placing groove (508), the bottom of the inner wall of the placing groove (11) is provided with the bottom buffer sponges (12), the side buffer sponges (10) are arranged on two sides of the inner wall of the placing groove (11), and culture dishes (9) are clamped on the inner sides of the side buffer sponges (10) and the tops of the bottom buffer sponges (12).
8. A cell subculture batch operation device according to claim 7, wherein: remove a buffer cylinder subassembly and include a sliding barrel (13), stopper (14), buffer spring (15) and an outer sliding barrel (16), a sliding barrel (13) is installed to the bottom of box (1), buffer spring (15) are installed at the interior top of a sliding barrel (13) on, an outer sliding barrel (16) is installed to the bottom of buffer spring (15), and an outer sliding barrel (16) cover is established the outside of (13), the top of base (8) is close to outer sliding barrel (16) department and installs stopper (14).
9. A method of performing a cell subculture batch culture according to claim 8, wherein: the method comprises the following steps:
step 1: firstly, placing the culture dish in the placing groove (11) by opening the switch door (3), and then closing the switch door (3) to enable the interior of the box body (1) to form a sealed state;
step 2: then, the temperature inside the box body (1) is detected through the temperature detector (513), so that the temperature inside the box body (1) is monitored in real time according to the temperature detector (513);
and step 3: when the temperature is too low, the second air pump (503) can be opened to pump the external air into the heater (505), then the air is heated and sprayed out through the nozzle (514), and the temperature in the box body (1) is kept at the set temperature;
and 4, step 4: when the temperature in the box body (1) is too high, air is pumped into the semiconductor cooler (6) by starting the air pump (6) so that the temperature in the box body (1) is kept constant;
and 5: meanwhile, the motor (501) is started to drive the rotating rod (510) to drive the fan blades (511) to rotate, so that air in the box body (1) continuously flows in a circulating mode;
step 6: the air passes through the drying filter screen (502) in the flowing process, and the moisture in the air passing through the drying filter screen (502) can be continuously absorbed, so that the drying degree in the box body (1) is ensured;
and 7: when the box body (1) needs to move, the box body moves through the universal wheels (18), and buffering is performed through the matching of the upper sliding barrel (13), the outer sliding barrel (16) and the buffering spring (15);
and 8: meanwhile, the limit of the culture dish (9) is buffered by the side buffer sponge (10) and the bottom buffer sponge (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111626707.XA CN114350513A (en) | 2021-12-28 | 2021-12-28 | Cell subculture batch operation device and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111626707.XA CN114350513A (en) | 2021-12-28 | 2021-12-28 | Cell subculture batch operation device and method |
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| CN114350513A true CN114350513A (en) | 2022-04-15 |
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| CN202111626707.XA Pending CN114350513A (en) | 2021-12-28 | 2021-12-28 | Cell subculture batch operation device and method |
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