CN201060128Y - Chromosome preparation optimizer - Google Patents
Chromosome preparation optimizer Download PDFInfo
- Publication number
- CN201060128Y CN201060128Y CNU2007201497977U CN200720149797U CN201060128Y CN 201060128 Y CN201060128 Y CN 201060128Y CN U2007201497977 U CNU2007201497977 U CN U2007201497977U CN 200720149797 U CN200720149797 U CN 200720149797U CN 201060128 Y CN201060128 Y CN 201060128Y
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- China
- Prior art keywords
- chromosome
- fan
- internal box
- chromosome preparation
- instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000349 chromosome Anatomy 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 36
- 238000001914 filtration Methods 0.000 claims description 15
- 238000005273 aeration Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 23
- 210000004027 cell Anatomy 0.000 abstract description 19
- 239000006185 dispersion Substances 0.000 abstract description 11
- 238000013461 design Methods 0.000 abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 30
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 238000001035 drying Methods 0.000 description 7
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 230000002559 cytogenic effect Effects 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 210000004748 cultured cell Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 230000031864 metaphase Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 206010067477 Cytogenetic abnormality Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 210000001776 amniocyte Anatomy 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000011066 ex-situ storage Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000009871 tenuigenin Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a chromosome preparation optimizing instrument, which comprises an outer box body and an inner box body, wherein a shape returning chamber is formed between the outer box body and the inner box body; the square-shaped chamber is internally provided with a temperature control device, a humidity control device and a fan; a plurality of drawers are arranged in the inner box body; the side walls of the two sides of the inner box body are respectively provided with a fan, and the bottom plate is a ventilating filter plate; the temperature control device, the humidity control device, the fan and the fan are connected with the controller in a unified mode. The direction and the speed of the dry temperature of control cell, humidity and the air current that the structural design of chromosome preparation optimizer can be accurate, and then control chromosome decentralized process is showing and is improving chromosome figure in middle stage, guarantees the highly uniform and the repeatability of best chromosome decentralized degree and chromosome dispersion, just the utility model discloses it is easy and simple to handle, preparation low cost has fine market prospect.
Description
Technical field
The utility model relates to a kind of Chromosome Preparation and optimizes instrument, and specifically a kind of Chromosome Preparation that is used for the chromosome decentralised control is optimized instrument.
Background technology
According to classical human cytogenetics, the chromosome that people's cell preparation goes out can be analyzed at microscopically, and the defective that may exist is diagnosed.In the past few decades, Cytogenetic techniques has obtained many-sided important breakthrough, as: introduce the hypotonic treatment technology of pair cell, used PHA to stimulate lymphocytic merisis, the cell synchronization culture technique, chromosome high resolution technology and original position amniocyte culture technique etc.Although aspect Cytogenetic techniques, obtained these great breakthroughs, but reach the chromosome degree of scatter of the best and the consistance and the repeatability of height thereof and be still a very problem of difficulty, very difficult consistance and repeatability that guarantees that each its chromosome of sample of analyzing as cytogenetics disperses.
For solving chromosome dispersion degree problem, cytogenetics expert has adopted a lot of methods, as: the slide of titration cell suspension is crossed spirit lamp flame or dripped sheet with wet slide; Improve to drip the height of sheet or with methods such as steam are stifling.These methods are difficult to reach the accurate control for condition, therefore not only have randomness in operating process, and these methods are still undesirable aspect consistance that guarantees the chromosome dispersion and optimum dispersion degree.
Method of chromosome preparation need be carried out processing such as hypotonic, fixing, dry, aging, enzymic digestion and apparent band to the cell of results, after these processing, just can analyze at the chromosome of microscopically pair cell, to judge whether to exist chromosome abnormality.This classic methods uses now at the beginning of setting up always.But in the chromosome analysis process of routine, often there are problems such as serious tenuigenin interference and hypotonic overlong time.There is influence in these problems to analytic process and result.
Generally speaking, a kind of instrument that can control the chromosome dust dispersion quality is extremely important for the clinical chromosome analysis that carries out.
Summary of the invention
The purpose of this utility model is to provide a kind of mid-term of drying after fixing processing the and Chromosome Preparation optimization instrument of prometaphase cell of being used for.Optimize chromosome dispersion degree uniformity after instrument is handled through preparation described in the utility model, the metaphase chromosome number is more, and chromosome resolution is higher, is easier to carry out microscopic analysis, and the heredity that may exist as diagnosis lacks etc.
For achieving the above object, the utility model adopts following technical scheme:
A kind of Chromosome Preparation is optimized instrument, and it comprises an outer case and an internal box, forms back-shaped chamber between outer case and the internal box; This back-shaped indoor attemperating unit, wet control device and blower fan of being provided with; Be provided with a plurality of drawers in the described internal box; Described internal box both sides sidewall is respectively equipped with fan so that air-supply is even, and base plate is the filter of aeration; Described attemperating unit, wet control device, blower fan are connected controller with fan is unified, and are provided with touch-screen in the outer case panel.Described fan is provided with independent switch on outer case.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, described controller also is connected with a Temperature Humidity Sensor.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, wherein, also is provided with the dividing plate of a band through hole between described internal box fan and drawer, forms the buffering cavity between this dividing plate and the internal box roof.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, the number of described drawer is 4.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, the base plate of described drawer is provided with several through holes.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, described fan is unidirectional Air Blast fan.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, described blower fan is unidirectional breeze fan.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, described outer case is provided with opening in this filter position, and opening part is provided with baffle plate.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, the filter of described internal box is the activated carbon filtration plate.
Above-mentioned a kind of Chromosome Preparation is optimized instrument, and wherein, described filter one side is provided with handle.
Using the utility model Chromosome Preparation optimization instrument to carry out dry cell can be through the in-situ method cultured cells, also can be the suspension cell of cultivating through the ex situ method.The original position cultured cells is after immobile liquid is handled, and directly slide or the Tissue Culture Flask with its adherent growth places drawer to carry out drying; Suspension cultured cells is dripped on slide after immobile liquid is handled, and slide is lain against carry out drying in the drawer then.
Chromosome Preparation optimization instrument described in the utility model in use blower fan can be controlled back-shaped indoor air-flow direction and speed, so that one-way movement is done in back-shaped indoor air wraparound shape chamber.Attemperating unit and wet control device are used for regulating back-shaped indoor air temperature and humidity, and attemperating unit and wet control device are connected with controller, can configure temperature required and humidity earlier on controller.Back-shapedly indoorly can also establish Temperature Humidity Sensor, in case back-shaped indoor air temperature humidity changes to some extent, Temperature Humidity Sensor will perceive at once and information is sent to controller, and controller can further be controlled temperature and humidity.The back-shaped indoor fan of air on the internal box sidewall evenly enters in the internal box, and the air communication of the cushion chamber formation uniformity on process internal box top is crossed drawer, cell on the slide of placing on the dry drawer-bottom board, the evaporation rate of control immobile liquid, and then control chromosomal dispersion degree, immobile liquid in the cell on all slides and the same slide diverse location evaporates with identical speed, thereby has guaranteed the consistance and the repeatability of chromosome dispersion degree in the Chromosome Preparation process.Can regulate and control the gas velocity of process drawer by fan on the internal box roof.Drawer-bottom board in the internal box is provided with several through holes, and the gas of the drawer of flowing through can further flow into the internal box bottom by this through hole.Contain a large amount of methyl alcohol and acetate in the cell fixation liquid, in the process of internal box inner drying, methyl alcohol and acetate evaporate in the inner loop air-flow, and these gases that contain methyl alcohol and acetate have very big harmful effect to experimenter's health.It is the activated carbon filtration plate of aeration that the utility model Chromosome Preparation is optimized instrument internal box base plate, the air-flow that contains acetate and methanol vapor behind the porous of the flowing through drawer, behind the activated carbon filtration plate, adsorbable methyl alcohol and the acetate wherein of activated charcoal, the air-flow that filters the back cleaning further enters back-shaped chamber and circulates, and has reached the dry run of pair cell with this reciprocation cycle.Outer case also is provided with opening at the activated carbon filtration Board position, and the filter sidewall also is provided with handle, can conveniently regularly replace filter like this, can use baffle plate to block opening when not changing.
Advantage of the present utility model and benefit:
The structural design of Chromosome Preparation optimization instrument described in the utility model can be controlled the direction and the speed of temperature, humidity and the air-flow of cell drying accurately.Samples such as peripheral blood, amniotic fluid, marrow or chorion after immobile liquid is fixing are optimized in the instrument through stable air-flow (stationary temperature, humidity and flow velocity) drying in Chromosome Preparation described in the utility model, cell fixation liquid is with suitable speed volatilization, can control the chromosome dispersion process, significantly improve the metaphase chromosome number, guarantee height consistance and repeatability that best chromosome degree of scatter and chromosome disperse.The design of the utility model activated carbon filtration plate can purify the circulating current in the preparation optimization instrument, has not only saved the energy, and has greatly reduced the injury of objectionable impurities to operating personnel.The utility model is easy and simple to handle, and cost of manufacture is cheap, has good market outlook.
Below in conjunction with accompanying drawing and preferred forms the utility model being described further, so that the public has whole and understanding fully to summary of the invention, and is not the qualification to the utility model protection domain.Aforementioned part fully discloses the protection domain that the utility model can be implemented, and therefore allly any well known in the artly is equal to replacement according to what the utility model disclosure was carried out, all belongs to infringement of the present utility model.
Description of drawings
Fig. 1 is the utility model external structure synoptic diagram;
Fig. 2 is the utility model inner structure synoptic diagram;
Fig. 3 is the utility model activated carbon filtration plate structure synoptic diagram;
Fig. 4 places the slide synoptic diagram for the utility model drawer-bottom board.
Embodiment
Shown in Fig. 1,2,3 and 4, the direction of arrow is that Chromosome Preparation is optimized airflow direction in the instrument among the figure.A kind of Chromosome Preparation is optimized instrument, and it comprises an outer case 1 and an internal box 2, forms back-shaped chamber 3 between outer case 1 and the internal box 2; Be provided with attemperating unit 4, wet control device 5 and blower fan 6 in this back-shaped chamber 3, and described attemperating unit 4, wet control device 5 and the blower fan 6 unified controllers 7 that are connected, controller 7 is provided with touch-screen 71 in outer case 1 panel, and described controller 7 also is connected with a Temperature Humidity Sensor 8; Be provided with four drawers 21 in the described internal box 2; Described internal box 2 sidewalls are respectively equipped with fan 22, and fan 22 is connected with controller 7, and fan 22 is provided with the independent regulation switch on outer case 1.Base plate is the activated carbon filtration plate 23 of aeration.
Also be provided with the dividing plate 24 of a band through hole between the above internal box 2 fan 22 and the drawer 21, form buffering cavity 25 between this dividing plate 24 and internal box 2 roofs.
As shown in Figure 4, the base plate of above-mentioned four drawers 21 is equipped with several through holes 211, and also is provided with handle 212 on the drawer 21.Said fans 22 is unidirectional Air Blast fan, and blower fan 6 is unidirectional breeze fan.
Above-mentioned outer case 1 is provided with opening 11 at the activated carbon filtration Board position, and opening 11 places are provided with baffle plate.As shown in Figure 3, activated carbon filtration plate 23 is provided with activated charcoal strainer 232 in the framework 231, and activated carbon filtration plate 23 1 sides are provided with handle 233.
Chromosome Preparation described in the utility model optimize instrument in use blower fan 6 can control air-flow direction and speed in the back-shaped chamber 3 so that one-way movement is done in the air wraparound shape chambers in the back-shaped chamber 3.Attemperating unit 4 and wet control device 5 are used for regulating air themperature and humidity in the back-shaped chamber 3, and attemperating unit 4 and wet control device 5 are connected with controller 7, can configure temperature required and humidity on controller 7 earlier.Can also establish Temperature Humidity Sensor 8 in the back-shaped chamber 3, in case air themperature humidity changes to some extent in the back-shaped chamber 3, Temperature Humidity Sensor 8 will perceive at once and information is sent to controller 7, and controller 7 can further be controlled temperature and humidity.Two fans 22 of air on internal box 2 sidewalls in the back-shaped chamber 3 enter in the internal box 2, and the air communication that the cushion chamber 25 of process internal box 2 forms uniformities is crossed drawer 21, cell on the slide of placing on drawer 21 base plates 9, the evaporation rate of control immobile liquid, and then control chromosomal dispersion degree, immobile liquid in the cell on all slides and the same slide diverse location evaporates with identical speed, thereby has guaranteed the consistance and the repeatability of chromosome dispersion degree in the Chromosome Preparation process.Can regulate and control the gas velocity of process drawer 21 by fan 22 on internal box 2 sidewalls.Drawer 21 base plates in the internal box 2 are provided with several through holes 211, and the gas of the drawer 21 of flowing through can further flow into internal box 2 bottoms by this through hole 211.Contain a large amount of methyl alcohol and acetate in the cell fixation liquid, in the process of internal box 2 inner dryings, methyl alcohol and acetate evaporate in the inner loop air-flow, and these gases that contain methyl alcohol and acetate have very big harmful effect to experimenter's health.Internal box 2 base plates that the utility model Chromosome Preparation is optimized instrument are the activated carbon filtration plate 23 of aeration, the air-flow that contains acetate and methanol vapor behind the porous of the flowing through drawer 21, behind activated carbon filtration plate 23, adsorbable methyl alcohol and the acetate wherein of activated charcoal, the air-flow that filters the back cleaning further enters back-shaped chamber 3 and circulates, and has reached the dry run of pair cell with this reciprocation cycle.Outer case 1 also is provided with opening 11 in activated carbon filtration plate 23 positions, and activated carbon filtration plate 23 sidewalls also are provided with handle 233, can conveniently regularly replace filter like this, can use baffle plate to block opening when not changing.
Claims (10)
1. a Chromosome Preparation is optimized instrument, it is characterized in that it comprises an outer case and an internal box, forms back-shaped chamber between outer case and the internal box; This back-shaped indoor attemperating unit, wet control device and blower fan of being provided with; Be provided with a plurality of drawers in the described internal box; Described internal box both sides sidewall is respectively equipped with fan, and base plate is the filter of aeration; Described attemperating unit, wet control device, blower fan and the unified controller that is connected of fan.
2. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that described controller also is connected with a Temperature Humidity Sensor.
3. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that, also is provided with the dividing plate of a band through hole between described internal box fan and drawer, forms cushion chamber between this dividing plate and the internal box roof.
4. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that the number of described drawer is 4.
5. optimize instrument according to claim 1 or 4 described a kind of Chromosome Preparation, it is characterized in that the base plate of described drawer is provided with several through holes.
6. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that described fan is unidirectional Air Blast fan.
7. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that described blower fan is unidirectional breeze fan.
8. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that described outer case is provided with opening in this filter position, and opening part is provided with baffle plate.
9. a kind of Chromosome Preparation according to claim 1 is optimized instrument, it is characterized in that the filter of described internal box is the activated carbon filtration plate.
10. optimize instrument according to claim 1,8 or 9 described a kind of Chromosome Preparation, it is characterized in that described filter one side is provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201497977U CN201060128Y (en) | 2007-06-22 | 2007-06-22 | Chromosome preparation optimizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201497977U CN201060128Y (en) | 2007-06-22 | 2007-06-22 | Chromosome preparation optimizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201060128Y true CN201060128Y (en) | 2008-05-14 |
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ID=39408607
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007201497977U Expired - Fee Related CN201060128Y (en) | 2007-06-22 | 2007-06-22 | Chromosome preparation optimizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201060128Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102589948A (en) * | 2012-02-29 | 2012-07-18 | 缪应江 | Biological sample processing box |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN104849121A (en) * | 2015-02-06 | 2015-08-19 | 上海乐辰生物科技有限公司 | Biological sample treating instrument |
-
2007
- 2007-06-22 CN CNU2007201497977U patent/CN201060128Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102589948A (en) * | 2012-02-29 | 2012-07-18 | 缪应江 | Biological sample processing box |
| CN102589948B (en) * | 2012-02-29 | 2014-07-09 | 缪应江 | Biological sample processing box |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN104849121A (en) * | 2015-02-06 | 2015-08-19 | 上海乐辰生物科技有限公司 | Biological sample treating instrument |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080514 Termination date: 20110622 |