CN102168014B - Cell culture drying test box - Google Patents
Cell culture drying test box Download PDFInfo
- Publication number
- CN102168014B CN102168014B CN 201110020198 CN201110020198A CN102168014B CN 102168014 B CN102168014 B CN 102168014B CN 201110020198 CN201110020198 CN 201110020198 CN 201110020198 A CN201110020198 A CN 201110020198A CN 102168014 B CN102168014 B CN 102168014B
- Authority
- CN
- China
- Prior art keywords
- space
- test
- gas flow
- flow modulation
- cell cultures
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000012360 testing method Methods 0.000 title claims abstract description 65
- 238000004113 cell culture Methods 0.000 title claims abstract description 18
- 238000001035 drying Methods 0.000 title claims abstract description 16
- 230000001105 regulatory effect Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000005057 refrigeration Methods 0.000 claims description 11
- 235000004443 Ricinus communis Nutrition 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 29
- 239000011521 glass Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
Abstract
The invention discloses a cell culture drying test box. The cell culture drying test box comprises a box body, a heater, a refrigerating system and a steam generator, wherein the box body is divided into a test space and an air flow regulating space by a baffle plate and the test space and the air flow regulating space are communicated with each other by air holes; a lighter and a test frame are arranged in the test space, and radiating fins of the heater and the refrigerating system are arranged in the air flow regulating space; and an air outlet of the steam generator is communicated with the air flow regulating space, and a circulation blower is arranged in the air flow regulating space. The test box can control the ambient temperature and humidity in the test space, the temperature and humidity are convenient and easy to regulate, and the test box is economical and practical. The test box can provide an environment with the most suitably controlled temperature and humidity for achieving a perfect chromosome dispersing effect. Under different combinations of temperature and humidity, the most suitable area can be obtained by using the test box.
Description
Technical field
The present invention relates to a kind of proofing box, particularly a kind of cell cultures drying test case.
Background technology
Original place and non-original place growth property cultivation results cell in period generate the slide glass oven dry need carry out under specific temperature and humidity; At present; The cell cultures drying test is to carry out in the conventional tests chamber; Temperature in the testing laboratory and temperature are difficult for accurately control, influences cell cultures drying test effect, are difficult to obtain highly consistent, ideal karyomit(e) distribution.
Summary of the invention
The problem that the present invention will solve is: the cell cultures drying test case that a kind of may command cell culture environment temperature and humidity is provided.
For addressing the above problem; The present invention includes casing, well heater, refrigeration system and vapour generator, casing is isolated test space and gas flow modulation space through dividing plate, and test space and gas flow modulation space are communicated with through pore; Be provided with head light and testing jig in the test space; The radiator element of well heater, refrigeration system is located in the gas flow modulation space, and the air outlet of vapour generator communicates with the gas flow modulation space, and gas flow modulation is provided with recirculation blower in the space.
For the ease of viewing test in the cell cultivation process, described casing is provided with the viewing window that communicates with test space, and viewing window and casing flexibly connect, and viewing window is provided with handle hole.
In order under the prerequisite that does not influence test operation, better casing outside atmosphere and test space to be isolated, described handle hole is provided with the rubber film of sandwich tape cross stitch.
In order in test space, to form convection current up and down; Make in the test space and can reach design temperature and humidity fast; Described gas flow modulation spatial loop is outside test space, and the gas flow modulation space is positioned at test space and communicates through breather hole with test space at the position of both sides up and down.
For the ease of the discharging water of condensation, described gas flow modulation spatial bottom is provided with the water shoot of band water discharge valve.
For the ease of adding water to vapour generator, described casing is provided with water tank, and water tank links to each other with the water pipe of vapour generator passband valve.
For the ease of regulating gas flow modulation spatial gas flow, described gas flow modulation is provided with blast fence in the space, and blast fence is connected with the barometric damper that the control blast fence rotates.
For the ease of ventilation inside and outside the casing, eliminating peculiar smell, described casing is provided with the vapor pipe that communicates with the gas flow modulation space, is provided with exhaust flap and extraction fan in the vapor pipe, and exhaust flap is connected with the exhaust regulating valve that the control exhaust flap rotates.
For conveniently moving, described bottom half is provided with castor.
For the ease of satisfying in-line power or other temporary line demand, the sidewall of said casing is provided with the cable entry port that communicates with test space.
Beneficial effect of the present invention: envrionment temperature and the humidity of proofing box of the present invention in can the control test space, and temperature and humidity of the present invention is easy to adjust, simple, economical and practical.The present invention scatters the temperature and humidity environment that effect provides optimum control for obtaining perfect karyomit(e).Under different temperature and humidity combination, can obtain the optimum term area that transfers to.But use in the proofing box routine work of the present invention to obtain highly consistent, ideal karyomit(e) and scatter, comprise that PHA stimulates lymphocyte, marrow, the original place of amino cell and fibroblast and the distribution of non-original place optimum dyeing body.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Among the figure: 1, exhaust regulating valve, 2, exhaust flap, 3, head light, 4, water tank, 5, recirculation blower; 6, well heater, 7, radiator element, 8, steam regulating valve, 9, level control valve, 10, vapour generator; 11, castor, 12, refrigeration system, 13, water discharge valve, 14, viewing window, 15, handle hole; 16, testing jig, 17, cable entry port, 18, barometric damper, 19, blast fence, 20, extraction fan.
Embodiment
A kind of specific embodiment as shown in Figure 1, it comprises casing, well heater 6, refrigeration system 12 and vapour generator 10, and bottom half is provided with castor 11, and the top of casing is provided with water tank 4.Casing is isolated test space, is looped around the outer gas flow modulation space of test space and is positioned at test space and the installation space of below, gas flow modulation space through dividing plate, and the gas flow modulation space is positioned at test space and communicates through breather hole with test space at the position of both sides up and down.Be provided with head light 3 and testing jig 16 in the test space, casing is provided with the viewing window 14 that communicates with test space, and viewing window 14 flexibly connects with casing, makes viewing window to open, and viewing window 14 is provided with handle hole 15.Handle hole 15 is provided with the rubber film of sandwich tape cross stitch.
The main frame of refrigeration system 12 and vapour generator 10 are installed in the installation space, and the air outlet of vapour generator 10 communicates through steam-pipe with the gas flow modulation space, and steam-pipe is provided with steam regulating valve 8.The water-in of vapour generator 10 links to each other with water tank 4 through water pipe, and water pipe is provided with level control valve 9.
The radiator element 7 of well heater 6 and refrigeration system 12 is installed in the gas flow modulation space, regulates gas flow modulation spatial temperature through well heater 6 and refrigeration system 12, regulates gas flow modulation space humidity through vapour generator 10.
Two recirculation blowers 5 also are installed in the gas flow modulation space, make air in the gas flow modulation space, to flow counterclockwise.Air exchanges through the gas in breather hole and the test space in flow process, makes that gas temperature and the humidity in the test space can be passed through well heater 6, refrigeration system 12 and vapour generator 10 controls.
Gas flow modulation spatial bottom is provided with the water shoot of band water discharge valve 13, and steam condensate can be discharged through water discharge valve 13 in the gas flow modulation space.
Be provided with blast fence 19 in the gas flow modulation space, blast fence 19 is connected with the barometric damper 18 that the control blast fence rotates.Flow through barometric damper 18 control gas flow modulation space empty gases.
Casing is provided with the vapor pipe that communicates with the gas flow modulation space, is provided with exhaust flap 2 and extraction fan 20 in the vapor pipe, and exhaust flap 2 is connected with the exhaust regulating valve 1 that control exhaust flap 2 rotates.
The sidewall of casing is provided with the cable entry port 17 that communicates with test space, is convenient to satisfy in-line power or other temporary line demand.Clog with stopper when not using.
This proofing box can be put a shelf at the half the height place of casing, puts to put the plate that is ready to slide glass is housed, and uses with convenient.In addition, proofing box can increase some other optional accessory, for example: vacuum pump, erlenmeyer flask, air extractor, refrigeration system tensimeter, disk recorder and computer interface.Operation window bottom convex part can be used as a storage zone easily, deposits the article that need use in the slide glass set-up procedure.
This proofing box also can be equipped with electronic regulator.Sort controller is simplified design to environmental test chamber.Unit is made up of tactile keyboard, four lines display screen, has made things convenient for the carrying out of programming and monitoring work.It has networking interface, and when starting the Web server function, long-range computingmachine can carry out PC control and record test data easily.
Claims (8)
1. cell cultures drying test case, it is characterized in that: comprise casing, well heater, refrigeration system and vapour generator, casing is isolated test space and gas flow modulation space through dividing plate; Test space and gas flow modulation space are communicated with through pore; Be provided with head light and testing jig in the test space, the radiator element of well heater, refrigeration system is located in the gas flow modulation space, and the air outlet of vapour generator communicates with the gas flow modulation space; Gas flow modulation is provided with recirculation blower in the space; Described gas flow modulation is provided with blast fence in the space, and blast fence is connected with the barometric damper that the control blast fence rotates, and described casing is provided with the vapor pipe that communicates with the gas flow modulation space; Be provided with exhaust flap and extraction fan in the vapor pipe, exhaust flap is connected with the exhaust regulating valve that the control exhaust flap rotates.
2. cell cultures drying test case according to claim 1, it is characterized in that: described casing is provided with the viewing window that communicates with test space, and viewing window and casing flexibly connect, and viewing window is provided with handle hole.
3. cell cultures drying test case according to claim 2, it is characterized in that: described handle hole is provided with the rubber film of sandwich tape cross stitch.
4. according to claim 1,2 or 3 described cell cultures drying test casees, it is characterized in that: described gas flow modulation spatial loop is outside test space, and the gas flow modulation space is positioned at test space and communicates through breather hole with test space at the position of both sides up and down.
5. cell cultures drying test case according to claim 4 is characterized in that: described gas flow modulation spatial bottom is provided with the water shoot of band water discharge valve.
6. cell cultures drying test case according to claim 4, it is characterized in that: described casing is provided with water tank, and water tank links to each other with the water pipe of vapour generator through the band valve.
7. cell cultures drying test case according to claim 4, it is characterized in that: described bottom half is provided with castor.
8. cell cultures drying test case according to claim 4, it is characterized in that: the sidewall of said casing is provided with the cable entry port that communicates with test space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110020198 CN102168014B (en) | 2011-01-18 | 2011-01-18 | Cell culture drying test box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110020198 CN102168014B (en) | 2011-01-18 | 2011-01-18 | Cell culture drying test box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102168014A CN102168014A (en) | 2011-08-31 |
| CN102168014B true CN102168014B (en) | 2012-12-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110020198 Active CN102168014B (en) | 2011-01-18 | 2011-01-18 | Cell culture drying test box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102168014B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102589948B (en) * | 2012-02-29 | 2014-07-09 | 缪应江 | Biological sample processing box |
| CN105333760A (en) * | 2015-11-17 | 2016-02-17 | 王丽 | Heat transmission rod of high-low-temperature alternating temperature humidity test chamber |
| CN106701574A (en) * | 2016-12-31 | 2017-05-24 | 宁波华仪宁创智能科技有限公司 | Cell culture operation integrated system and working method thereof |
| CN111176353A (en) * | 2020-01-06 | 2020-05-19 | 宁波送变电建设有限公司永耀科技分公司 | Temperature and humidity adjusting device for salt spray experiment machine |
| CN111690535A (en) * | 2020-06-24 | 2020-09-22 | 重庆智领医创科技成果转化服务有限责任公司 | Cell test observation box |
| JP2022168955A (en) * | 2021-04-27 | 2022-11-09 | エスペック株式会社 | incubator |
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| CN100999709A (en) * | 2006-12-27 | 2007-07-18 | 甘宜梧 | Biological safety incubator |
| CN101342499A (en) * | 2008-08-18 | 2009-01-14 | 济南鑫贝西生物技术有限公司 | Two-sided low-temperature biological operation bench |
| CN101342500A (en) * | 2008-08-20 | 2009-01-14 | 济南鑫贝西生物技术有限公司 | Low-temperature biological operation bench |
| CN201244484Y (en) * | 2008-08-20 | 2009-05-27 | 济南鑫贝西生物技术有限公司 | Low-temperature biological operation bench |
| CN201493120U (en) * | 2009-03-05 | 2010-06-02 | 山东博科生物产业有限公司 | Air-makeup biological safety cabinet |
| WO2011048985A1 (en) * | 2009-10-23 | 2011-04-28 | 株式会社日立ハイテクノロジーズ | Gas temperature/humidity regulation method and gas supply device |
| CN201981203U (en) * | 2011-01-18 | 2011-09-21 | 山东博科生物产业有限公司 | Cell culture drying test box |
-
2011
- 2011-01-18 CN CN 201110020198 patent/CN102168014B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999709A (en) * | 2006-12-27 | 2007-07-18 | 甘宜梧 | Biological safety incubator |
| CN101342499A (en) * | 2008-08-18 | 2009-01-14 | 济南鑫贝西生物技术有限公司 | Two-sided low-temperature biological operation bench |
| CN101342500A (en) * | 2008-08-20 | 2009-01-14 | 济南鑫贝西生物技术有限公司 | Low-temperature biological operation bench |
| CN201244484Y (en) * | 2008-08-20 | 2009-05-27 | 济南鑫贝西生物技术有限公司 | Low-temperature biological operation bench |
| CN201493120U (en) * | 2009-03-05 | 2010-06-02 | 山东博科生物产业有限公司 | Air-makeup biological safety cabinet |
| WO2011048985A1 (en) * | 2009-10-23 | 2011-04-28 | 株式会社日立ハイテクノロジーズ | Gas temperature/humidity regulation method and gas supply device |
| CN201981203U (en) * | 2011-01-18 | 2011-09-21 | 山东博科生物产业有限公司 | Cell culture drying test box |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102168014A (en) | 2011-08-31 |
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| PB01 | Publication | ||
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Cell culture drying test chamber Effective date of registration: 20211101 Granted publication date: 20121205 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
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Date of cancellation: 20220624 Granted publication date: 20121205 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Cell culture drying test chamber Effective date of registration: 20230206 Granted publication date: 20121205 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
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Date of cancellation: 20230531 Granted publication date: 20121205 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
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