CN210683808U

CN210683808U - Stem cell incubator

Info

Publication number: CN210683808U
Application number: CN201921572222.5U
Authority: CN
Inventors: 任建华
Original assignee: Zhengzhou Huayan Hezhong Biotechnology Co ltd
Current assignee: Zhengzhou Huayan Hezhong Biotechnology Co ltd
Priority date: 2019-09-20
Filing date: 2019-09-20
Publication date: 2020-06-05
Anticipated expiration: 2029-09-20

Abstract

The utility model relates to the technical field of cell culture, in particular to a stem cell incubator, which comprises a case body, wherein a case door is movably connected on the case body; the box body comprises an outer box body and an inner box body, a culture inner cavity is arranged in the inner box body, air outlet areas are arranged at the peripheral edges of the rear wall of the inner box body, a boss protruding towards the culture inner cavity is arranged in the middle of the rear wall of the inner box body, an air inlet is formed in the boss, and an HEPA filter screen is detachably connected to the air inlet; the air-conditioning device is characterized in that a hot air cavity is arranged between the rear wall of the inner box body and the rear wall of the outer box body, a fan is arranged in the hot air cavity, a heating pipe is arranged at an air outlet of the fan, the air outlet of the fan is communicated with the culture inner cavity through an air inlet, and the air outlet area is communicated with the hot air cavity. The utility model discloses stem cell incubator simple structure, reasonable in design, the convection current of hot-air in the culture inner chamber is effectual, and the temperature of culture inner chamber is even, stable, and the survival rate of cell is high.

Description

Stem cell incubator

Technical Field

The utility model belongs to the technical field of cell culture technique and specifically relates to a stem cell incubator.

Background

Stem cells are a class of cells with proliferative and differentiative potential, have the ability to self-renew and replicate, and under certain conditions, can differentiate into a variety of highly differentiated functional cells. The humidity and temperature of the culture environment during the in vitro culture of stem cells are strict, so that the culture is usually carried out in a cell culture box. The existing stem cell incubator comprises a water jacket type incubator and a gas jacket type incubator. When the temperature of the water jacket type incubator is kept constant, the independent water jacket layer surrounds the inner box body, and the water in the water jacket is heated through the electric heating wire, so that the temperature in the incubator is constant at the set temperature. The water jacket type incubator has good temperature uniformity, can well slow down the reduction of the temperature in the incubator after power failure, but has slow heating and long temperature rise process, and needs a long time to raise the temperature of the incubator to the set temperature after being restarted after power failure; moreover, frequent opening and closing of the box door has a large influence on the temperature inside the incubator. The heating of gas jacket formula carbon dioxide incubator is directly heated the box through spreading over the intraformational heater of box gas jacket, has the heating fast, and the intensification is rapid, and temperature regulation resumes fast characteristics, can reach the cultivation temperature of settlement fast after the incubator is opened. However, most of the existing air-jacketed carbon dioxide incubators have poor convection effect of hot air in the incubator body, the temperature near the air jacket layer is high, the temperature far away from the air jacket layer is low, the temperature in the incubator is uneven, and the temperature difference exists at different parts, thereby affecting the cell culture effect.

In order to solve the problem of uneven temperature in the incubator due to poor convection effect of hot air in the incubator, some incubators improve the convection effect of hot air circulation in the incubator by additionally arranging a fan, an air outlet hole and an air inlet hole, but the existing incubator provided with the fan, the air outlet hole and the air inlet hole, the air outlet and the air inlet are usually arranged in the middle of the rear wall of the box body in the box body, the air inlet and the air outlet are distributed on the same plane, the air outlet is arranged at the periphery of the air inlet and is arranged at intervals with the air inlet, this setting leads to the fresh air inlet closer with the interval of exhaust vent, leads to easily blowing out from the exhaust vent to the hot-blast chamber that just is inhaled immediately by the fresh air inlet and circulates from the exhaust vent to blow off, causes hot-blast circulation path in the incubator inner chamber short, and incubator inner chamber middle part temperature is even, and the temperature and the middle part difference in temperature of edge and corner are big.

SUMMERY OF THE UTILITY MODEL

To the problem that prior art exists with not enough, the utility model aims at providing a stem cell incubator.

The utility model adopts the technical proposal that:

a stem cell incubator comprises a incubator body, wherein a chamber door is movably connected to the incubator body; the box body comprises an outer box body and an inner box body, a culture inner cavity is arranged in the inner box body, air outlet areas are arranged at the peripheral edges of the rear wall of the inner box body, a boss protruding towards the culture inner cavity is arranged in the middle of the rear wall of the inner box body, an air inlet is formed in the boss, and an HEPA filter screen is detachably connected to the air inlet; the air-conditioning device is characterized in that a hot air cavity is arranged between the rear wall of the inner box body and the rear wall of the outer box body, a fan is arranged in the hot air cavity, a heating pipe is arranged at an air outlet of the fan, the air outlet of the fan is communicated with the culture inner cavity through an air inlet, and the air outlet area is communicated with the hot air cavity.

According to the above stem cell incubator, preferably, the boss at the edge of the air inlet is provided with an annular clamping groove surrounding the air inlet, and the HEPA filter network card is arranged in the annular clamping groove.

According to foretell stem cell incubator, preferably, HEPA filter screen outside can dismantle and be connected with hot-blast cover, evenly distributed has a plurality of fresh air inlets on the hot-blast cover.

According to foretell stem cell incubator, preferably, evenly distributed has a plurality of exhaust vents in the exhaust zone, the exhaust vent communicates with hot-blast cavity.

According to the stem cell incubator, preferably, a humidifying disc is arranged on the bottom wall of the inner box body.

According to the stem cell incubator, preferably, the left side wall and the right side wall of the inner box body are both provided with fixed blocks, the fixed blocks are movably connected with bearing plates, and the bearing plates are of a screen plate structure.

According to the stem cell incubator, preferably, the side wall of the inner box body is provided with an ultraviolet sterilizing lamp.

According to the stem cell incubator, preferably, the outer box body is provided with an asbestos heat insulation layer.

According to the above stem cell incubator, preferably, the chamber door has a double-layer door structure, and includes an inner layer door and an outer layer door, and an air heat insulation layer is provided between the inner layer door and the outer layer door.

According to the stem cell incubator, preferably, the rear wall of the outer box body is provided with heat dissipation holes corresponding to the fan for heat dissipation of the fan.

According to the stem cell incubator, preferably, transparent visual windows are formed on the inner layer door and the outer layer door.

The utility model discloses the beneficial effect who gains does:

(1) the utility model discloses in incubator on the box back wall air intake district and exhaust zone not on a plane, the air intake district sets up on the boss at inner box back wall middle part, the exhaust zone spreads all over the edge all around at inner box back wall, this setting has increased the spacing distance between air intake district and the exhaust zone, the hot-blast that blows into in the cultivation inner chamber from the air intake district reaches incubator chamber door and turns back, then is inhaled in the hot-blast cavity through each exhaust vent that establishes around inner box back wall, heats once more, thereby accomplishes a heated air circulation, therefore, the utility model discloses a dry cell incubator who protects has increased the interval between air intake district and the exhaust zone, has prolonged the circulation path of hot-blast in the cultivation inner chamber, makes the air intake blow into the hot-blast that cultivates the inner chamber can spread all over each corner of cultivation inner chamber, has improved the convection effect of hot-air in the incubator, has improved thermal distribution homogeneity and equilibrium in the cultivation inner chamber, the temperature of the culture cavity is ensured to be uniform and stable, and the survival rate of stem cells is improved.

(2) The utility model discloses be equipped with the HEPA filter screen on the air intake of stem cell incubator, pollutants such as smog, dust, bacterium in the HEPA filter screen can the filter-removal air play to getting into filtration, purification, the degerming effect of incubator air, guarantee the aseptic culture environment in the incubator, reduced the probability that the cell received the pollution among the process of cultivateing, improve the cultivation quality and the survival rate of cell. And the HEPA filter screen card is arranged on the air inlet, so that the HEPA filter screen can be cleaned and replaced conveniently.

(3) The utility model discloses the outside of HEPA filter screen is equipped with hot-blast cover in the stem cell incubator, and hot-blast cover plays the effect of protection HEPA filter screen, prevents to cultivate in-process culture container and HEPA filter screen direct contact or collision and leads to the HEPA filter screen to damage.

(4) The utility model discloses stem cell incubator's outer box is equipped with the asbestos heat preservation, and the asbestos heat preservation can have the outer box of reduction and takes place the heat exchange with external environment, reduces calorific loss, also reduces external environment temperature simultaneously and changes the influence to incubator internal culture temperature, maintains incubator internal culture temperature invariant.

(5) The utility model discloses stem cell incubator's chamber door is double-layer door structure, including inlayer door and outer door, be equipped with the air insulation layer between inlayer door and the outer door, should set up and to reduce chamber door and external environment's heat exchange, play thermal-insulated heat preservation effect, prevent that the heat from scattering and disappearing, be favorable to maintaining the incubator in the cultivation constancy of temperature.

(6) The utility model discloses the louvre has been seted up with the corresponding position of fan on the outer box back wall of stem cell incubator, and this louvre can be used to the heat dissipation of motor in the fan for at the in-process of incubator work, the heat of the production of motor can in time be discharged through the louvre, realizes the cooling of motor, and the motor of protection fan normally works, prolongs the life of incubator.

(7 the utility model discloses stem cell incubator simple structure, reasonable in design cultivates that the convection current of hot-air is effectual in the inner chamber, cultivates that the temperature of inner chamber is even, stable, and the survival rate of cell is high.

Drawings

FIG. 1 is a schematic structural view of a stem cell incubator according to embodiment 1 of the present invention;

FIG. 2 is a schematic structural diagram of a box body of a stem cell incubator according to embodiment 1 of the present invention (the box door is not shown in the figure);

FIG. 3 is a cross-sectional view taken along A-A of the stem cell incubator body shown in FIG. 2 (the fixing block is not shown);

FIG. 4 is a schematic structural diagram of a stem cell incubator according to embodiment 2 of the present invention (the chamber door is not shown);

FIG. 5 is a cross-sectional view taken along A-A of the stem cell incubator body shown in FIG. 4 (the fixing block is not shown);

in the figure: the box is characterized in that the box body is 1, the box door is 2, the handle is 3, the control panel is 4, the outer box body is 5, the inner box body is 6, the air outlet hole is 7, the boss is 8, the hot air cover is 9, the air inlet hole is 10, the fixing lug is 11, the ultraviolet germicidal lamp is 12, the humidifying disc is 13, the HEPA filter screen is 14, the fan is 15, the heating pipe is 16, and the fixing block is 17.

Detailed Description

The present invention is further described in detail below with reference to specific examples, but the scope of the present invention is not limited thereto.

Example 1:

a stem cell incubator, see fig. 1-3, comprises an incubator body 1, wherein a chamber door 2 is hinged on the incubator body 1; the box body 1 comprises an outer box body 5 and an inner box body 6, the outer box body 5 is provided with an asbestos heat insulation layer, the upper part of the outer box body 5 is provided with a control panel 4, a culture inner cavity is arranged inside the inner box body 6, air outlet areas are arranged at the peripheral edges of the rear wall of the inner box body 6, and a plurality of air outlet holes 7 are uniformly distributed in the air outlet areas; 6 back wall middle parts of interior box are equipped with to cultivateing the bellied boss 8 of inner chamber, be equipped with the air intake on the boss 8, set up the ring groove who encircles the air intake on the boss 8 at air intake edge, the card is equipped with HEPA filter screen 14 in the draw-in groove, and HEPA filter screen 14 card is established on the air intake, the HEPA filter screen 14's of being convenient for washing, change. The bottom wall of the inner box body 6 is provided with a humidifying disc 13, the humidifying disc 13 can humidify the culture inner cavity, and the humidity of the culture inner cavity is ensured to meet the humidity requirement of stem cell culture; all be equipped with fixed block 17 on the left side wall and the right side wall of interior box 6, swing joint has the dish that bears (not drawn in the picture) that is used for placing the cell culture container on fixed block 17, bear the grid plate structure that the dish is the fretwork, be favorable to the diffusion of moisture and hot-air in cultivateing the inner chamber, moreover, all be equipped with sterilamp 12 on the both sides wall of interior box 6, sterilamp 12 can carry out germicidal treatment to incubator inside, ensure that incubator inside is in sterile condition, can effectively prevent that the cell of cultivateing from receiving the pollution.

A hot air cavity is arranged between the rear wall of the inner box body 6 and the rear wall of the outer box body 5, a fan 15 is arranged in the hot air cavity, a heating pipe 16 is arranged at an air outlet of the fan 15, the air outlet of the fan 15 is communicated with the culture inner cavity through an air inlet, and the air outlet 7 is communicated with the hot air cavity. The rear wall of the outer box body 5 is provided with heat dissipation holes corresponding to the fan 15 for heat dissipation of the fan 15.

The box door 2 is of a double-layer door structure and comprises an inner layer door and an outer layer door, an air heat insulation layer is arranged between the inner layer door and the outer layer door, and the double-layer door structure can reduce heat exchange between the box door 2 and the external environment, plays a role in heat insulation and preservation, prevents heat from dissipating and is beneficial to maintaining constant culture temperature in the incubator; be equipped with handle 3 on the outer door, be convenient for switch chamber door 2, all begin to have transparent visual window on inlayer door and the outer door, be convenient for observe the cultivation condition of stem cell in the incubator.

Example 2:

the content of example 2 is substantially the same as that of example 1, except that: 14 outsides of HEPA filter screen are equipped with hot-blast cover 9, evenly laid a plurality of fresh air inlets 10 on the hot-blast cover 9, be equipped with fixed ear 11 on the hot-blast cover 9, fixed ear 11 and the boss 8 of hot-blast cover 9 pass through the bolt can dismantle the connection (refer to figure 3 and figure 4), hot-blast cover 9 can prevent to cultivate in-process culture container and HEPA filter screen 14 direct contact or collision and lead to HEPA filter screen 14 to damage, thereby play the effect of protection HEPA filter screen 14, moreover, fixed ear 11 and the boss 8 of hot-blast cover 9 pass through the bolt can dismantle the connection, the washing of the hot-blast cover 9 of being convenient for, the washing and the change of HEPA filter screen 14 of also being convenient.

The hot air circulation process in the stem cell incubator of the utility model is as follows:

air in the hot air cavity is sucked by the fan 15, hot air is formed after the air is heated by the heating pipe 16, the hot air is blown out from an air outlet of the fan 15 and enters the culture cavity through an air inlet of the rear wall of the inner box body 6 and the HEPA filter screen 14, meanwhile, a negative pressure state is formed in the hot air cavity due to outflow of the hot air from the air inlet, and the air in the culture cavity is favorably sucked into the hot air cavity from air outlet holes 7 at the peripheral edge of the rear wall of the inner box body 6; the hot air blown into the culture cavity from the air inlet reaches the door 2 of the incubator and then turns back, and is sucked into the hot air cavity through the air outlet holes 7 arranged at the peripheral edges of the rear wall of the inner box body 6, and then is heated again, so that a hot air circulation is completed. The utility model discloses a stem cell incubator has increased the interval between intake zone and the exhaust zone, has prolonged hot-blast circulation route in cultivateing the inner chamber for the hot-blast each corner of cultivateing the inner chamber that can spread all over of air intake blowing, improved the convection current effect of hot-air in the incubator, improved the heat distribution homogeneity and the equilibrium of cultivateing in the inner chamber, the cultivation temperature of ensureing to cultivate the inner chamber is even, stable, has improved the survival rate of stem cell.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and is not to be construed as limiting the invention to the above embodiments, but is intended to cover all modifications, equivalents, and improvements made within the spirit and principles of the present invention.

Claims

1. A stem cell incubator is characterized by comprising an incubator body, wherein a chamber door is movably connected to the incubator body; the box body comprises an outer box body and an inner box body, a culture inner cavity is arranged in the inner box body, air outlet areas are arranged at the peripheral edges of the rear wall of the inner box body, a boss protruding towards the culture inner cavity is arranged in the middle of the rear wall of the inner box body, an air inlet is formed in the boss, and an HEPA filter screen is detachably connected to the air inlet; the air-conditioning device is characterized in that a hot air cavity is arranged between the rear wall of the inner box body and the rear wall of the outer box body, a fan is arranged in the hot air cavity, a heating pipe is arranged at an air outlet of the fan, the air outlet of the fan is communicated with the culture inner cavity through an air inlet, and the air outlet area is communicated with the hot air cavity.

2. The stem cell incubator according to claim 1, wherein the boss on the edge of the air inlet is provided with an annular clamping groove surrounding the air inlet, and the HEPA filter network card is arranged in the annular clamping groove.

3. The stem cell incubator according to claim 2, wherein a hot air hood is detachably connected to the outer side of the HEPA filter screen, and a plurality of air inlet holes are uniformly distributed on the hot air hood.

4. The stem cell incubator according to any one of claims 1 to 3, wherein a plurality of air outlet holes are uniformly arranged in the air outlet region, and the air outlet holes are communicated with the hot air chamber.

5. The stem cell incubator of claim 4 wherein the inner chamber body has a humidifying disc on a bottom wall thereof.

6. The stem cell incubator according to claim 5, wherein the left side wall and the right side wall of the inner box body are both provided with fixed blocks, the fixed blocks are movably connected with bearing plates, and the bearing plates are in a screen plate structure.

7. The stem cell incubator of claim 6 wherein the inner chamber sidewall is provided with a UV germicidal lamp.

8. The stem cell incubator of claim 7 wherein the outer housing is provided with an asbestos insulation.

9. The stem cell incubator of claim 8, wherein the chamber door is a double-layer door structure comprising an inner layer door and an outer layer door, and an air heat insulation layer is arranged between the inner layer door and the outer layer door.

10. The stem cell incubator of claim 9, wherein the rear wall of the outer housing is provided with heat dissipation holes at positions corresponding to the fans.