CN114308405A - Interleukin is extracted inclusion body separator that can ration ratio - Google Patents
Interleukin is extracted inclusion body separator that can ration ratio Download PDFInfo
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- CN114308405A CN114308405A CN202111680972.6A CN202111680972A CN114308405A CN 114308405 A CN114308405 A CN 114308405A CN 202111680972 A CN202111680972 A CN 202111680972A CN 114308405 A CN114308405 A CN 114308405A
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Abstract
The invention relates to the technical field of interleukin extraction, in particular to an inclusion body separation device capable of quantitatively proportioning interleukin extraction, which comprises a barrel, wherein an annular groove is formed in the inner wall of the barrel close to the middle, limiting grooves are formed in the inner wall of the barrel close to the top end and the bottom end of the annular groove, four groups of sliding blocks are arranged on the inner wall of the barrel close to the top end, a motor is arranged on the inner wall of the bottom end of the barrel close to the middle, a placing table is connected to the output end of the motor in a transmission manner, extension rods are arranged on two sides of the placing table, balls are arranged at one ends of two groups of extension rods, limiting rods are arranged at the top ends and the bottom ends of the two groups of extension rods, a placing cylinder is arranged at the top end of the placing table, and two groups of annular sliding rails are arranged on the outer surface of the placing cylinder. The problem that the separation barrel and the centrifugal barrel are influenced to normally extract due to the fact that the barrel is easily shaken in the centrifugal extraction process can be effectively solved under the setting action of parts such as a limiting groove, a sliding block, an extension rod, a ball, a limiting rod and an annular sliding rail.
Description
Technical Field
The invention relates to the technical field of interleukin extraction, in particular to an inclusion body separation device capable of quantitatively proportioning interleukin extraction.
Background
Interleukins, abbreviated as interleukins, refer to lymphokines which interact between leukocytes or immune cells and which belong to the same cytokine as blood cell growth factors. The two are mutually coordinated and interacted to jointly complete the functions of hematopoiesis and immunoregulation. Interleukins play an important role in transmitting information, activating and regulating immune cells, mediating T, B cell activation, proliferation and differentiation and in inflammatory responses;
the inclusion body is a high-density insoluble protein particle which is formed by being wrapped by a membrane when an exogenous gene is expressed in a prokaryotic cell, particularly when the exogenous gene is efficiently expressed in escherichia coli, and is a high-refraction area when observed under a microscope, and is obviously different from other components in cytoplasm;
the inclusion body needs to be quantitatively proportioned in the process of extracting the interleukin, and a special separating device capable of quantitatively proportioning is needed at the moment, but the existing separating device has a plurality of problems and needs to be further improved.
The existing inclusion body separating device capable of quantitatively proportioning for extracting interleukin has the problem that a placing cylinder is easy to shake in the centrifugal extraction process so as to influence the normal extraction of the separating cylinder and a centrifugal cylinder, and inconvenience is brought to the actual operation; the invention provides an inclusion body separating device capable of quantitatively proportioning interleukin, which is used for solving the problem that a separating cylinder and a centrifugal cylinder cannot be conveniently and quickly disassembled and culture solution cannot be extracted after the extraction operation is finished.
Disclosure of Invention
The present invention aims to provide an inclusion body separation device capable of quantitatively proportioning for interleukin extraction, so as to solve the problems proposed in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: an inclusion body separation device capable of quantitatively proportioning for extracting interleukin comprises a barrel body, wherein an annular groove is formed in the inner wall of the barrel body by the middle position, limiting grooves are formed in the inner wall of the barrel body by the top end and the bottom end of the annular groove, four groups of sliding blocks are arranged on the inner wall of the barrel body by the top end, a motor is arranged on the inner wall of the bottom end of the barrel body by the middle position, an output end of the motor is in transmission connection with a placing table, extending rods are arranged on two sides of the placing table, balls are arranged at one ends of two groups of the extending rods, the limiting rods are arranged on the top ends and the bottom ends of the two groups of the extending rods, a placing barrel is arranged at the top end of the placing table, two groups of annular sliding rails are arranged on the outer surface of the placing barrel, a mounting seat is arranged on the inner wall of the bottom end of the placing barrel, a mounting groove is formed in the mounting seat, and a magnet block is arranged on the inner wall of the bottom end of the mounting groove, the novel centrifugal separation device is characterized in that a baffle is mounted in the position, close to the bottom, of the interior of the placing barrel, a through groove is formed in the position, close to the middle, of the interior of the baffle, a sealing ring is arranged in the through groove, an annular rubber strip is arranged at the top end of the through groove, the top surface of the magnet block is fixedly attached to the bottom end of the iron rod, the top end of the iron rod is fixedly connected with the bottom end of the separating barrel, four groups of fixing plates are mounted on the outer surface of the separating barrel, close to the top end, of the separating barrel, fixing bolts are movably screwed in the four groups of fixing plates, threaded columns are mounted on the inner wall of the bottom end of the separating barrel, close to the middle, of the threaded columns, are movably screwed in the outer surface of the threaded columns, a centrifugal barrel is arranged on the inner wall of the centrifugal barrel, and a water permeable net is arranged on the position, close to the bottom end, of the inner wall of the centrifugal barrel.
Preferably, the sizes of the two groups of balls are equal, and the outer surfaces of the two groups of balls are attached to the inner wall of the corresponding side of the annular groove.
Preferably, the number of the limiting rods is four, the four limiting rods are equal in size, and one ends of the four limiting rods are respectively located in the corresponding limiting grooves.
Preferably, the four groups of sliding blocks are equal in size, the four groups of sliding blocks are arranged in a centrosymmetric mode by taking the center point of the cylinder as a central point, and the outer surfaces of the corresponding sides of the four groups of sliding blocks are in sliding connection with the annular sliding rail.
Preferably, the four groups of fixing plates are equal in size, and are arranged in a central symmetry manner by taking the central point of the separating cylinder as a central point.
Preferably, the top end of the placing cylinder is provided with thread grooves close to the positions corresponding to the fixing bolts, and the bottom ends of the four groups of fixing bolts are movably connected with the corresponding thread grooves in a threaded manner.
Preferably, the internal diameter size of sealing washer and the external diameter size looks adaptation of iron system pole, the internal diameter size of mounting groove and the external diameter size looks adaptation strip of iron system pole.
Preferably, the two groups of extension rods are equal in size, and the thickness of the two groups of extension rods is matched with the distance between the inner wall of the top end of the annular groove and the inner wall of the bottom end of the annular groove.
Compared with the prior art, the invention has the beneficial effects that:
1. the problem that the separation barrel and the centrifugal barrel are influenced to normally extract due to the fact that the barrel is easily shaken in the centrifugal extraction process can be effectively solved under the setting action of parts such as a limiting groove, a sliding block, an extension rod, a ball, a limiting rod and an annular sliding rail.
2. Through the setting of spare parts such as mount pad, mounting groove, magnet piece, fixed plate, fixing bolt, iron system pole and screw thread post, can effectual solution draw the operation and finish the back, can not convenient, swift dismantle separating cylinder and centrifuge tube and carry out the problem of drawing the culture solution.
Drawings
FIG. 1 is a schematic structural view of the main body of the present invention;
FIG. 2 is a schematic structural section of the barrel of the present invention;
FIG. 3 is an enlarged view of the point A in FIG. 2;
FIG. 4 is an enlarged view of the point B in FIG. 2;
FIG. 5 is a perspective view of the placement platform of the present invention;
fig. 6 is a schematic perspective view of the slider according to the present invention.
In the figure: 1. a barrel; 11. an annular groove; 12. a limiting groove; 13. a slider; 2. a motor; 21. a placing table; 22. an extension rod; 23. a ball bearing; 24. a limiting rod; 3. placing the cylinder; 31. an annular slide rail; 32. a mounting seat; 33. mounting grooves; 34. a magnet block; 35. a baffle plate; 36. a through groove; 37. a seal ring; 38. an annular rubber strip; 4. a separation cylinder; 41. a fixing plate; 42. fixing the bolt; 43. an iron rod; 44. a threaded post; 5. a centrifugal cylinder; 51. a water permeable net.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 6, the present invention provides a technical solution: an inclusion body separating device for interleukin extraction and quantitative proportioning comprises a cylinder body 1, wherein an annular groove 11 is arranged at the position close to the middle of the inner wall of the cylinder body 1, limiting grooves 12 are respectively arranged at the positions close to the top end and the bottom end of the annular groove 11 on the inner wall of the cylinder body 1, four groups of sliding blocks 13 are arranged at the position close to the top end on the inner wall of the cylinder body 1, the sizes of the four groups of sliding blocks 13 are equal, the four groups of sliding blocks 13 are arranged in central symmetry by taking the central point of the cylinder body 1 as the central point, the outer surfaces of the corresponding sides of the four groups of sliding blocks 13 are in sliding connection with an annular sliding rail 31, a motor 2 is arranged at the position close to the middle of the inner wall of the bottom end of the cylinder body 1, the output end of the motor 2 is in transmission connection with a placing table 21, extension rods 22 are arranged at the two sides of the placing table 21, the sizes of the two groups of extension rods 22 are equal, and the thicknesses of the two groups of the extension rods 22 are matched with the distance between the inner wall of the top end and the inner wall of the annular groove 11, the ball 23 is installed at one end of two groups of extension rods 22, the two groups of balls 23 are equal in size, the outer surfaces of the two groups of balls 23 are attached to the inner wall of the corresponding side of the annular groove 11, the limiting rods 24 are installed at the top ends and the bottom ends of the two groups of extension rods 22, the limiting rods 24 are four groups, the four groups of limiting rods 24 are equal in size, one ends of the four groups of limiting rods 24 are respectively located in the corresponding limiting grooves 12, the placing barrel 3 is installed at the top end of the placing table 21, two groups of annular slide rails 31 are installed on the outer surface of the placing barrel 3, the installing seat 32 is installed on the inner wall of the bottom end of the placing barrel 3, the installing seat 32 is internally provided with an installing groove 33, the magnet block 34 is installed on the inner wall of the bottom end of the placing barrel 3, the baffle 35 is installed at the position close to the bottom end of the placing barrel 3, the penetrating groove 36 is formed at the position close to the middle of the inside of the baffle 35, the sealing ring 37 is arranged inside of the penetrating groove 36, the inner diameter of the sealing ring 37 is matched with the outer diameter of the iron rod 43, the inner diameter of the mounting groove 33 is matched with the outer diameter of the iron rod 43, the top end of the through groove 36 is provided with an annular rubber strip 38, the top surface of the magnet block 34 is fixedly adsorbed with the bottom end of the iron rod 43, the top end of the iron rod 43 is fixed with the bottom end of the separating cylinder 4, four groups of fixing plates 41 are arranged on the outer surface of the separating cylinder 4 near the top end, the sizes of the four groups of fixing plates 41 are equal, the four groups of fixing plates 41 are arranged in central symmetry by taking the central point of the separating cylinder 4 as the central point, the fixing bolts 42 are movably screwed in the four groups of fixing plates 41, threaded grooves are formed in the top end of the placing cylinder 3 near the corresponding fixing bolts 42, the bottom ends of the four groups of fixing bolts 42 are respectively movably screwed with the corresponding threaded grooves, threaded columns 44 are arranged on the inner wall of the bottom end of the separating cylinder 4 near the middle position, the outer surface of the threaded column 44 is movably screwed with a centrifugal cylinder 5, and a water permeable net 51 is arranged at the position close to the bottom end of the inner wall of the centrifugal cylinder 5;
the working principle is as follows: in the using process, a user can place the culture solution containing interleukin in the centrifugal cylinder 5, the motor 2 can be started at the moment, the placing table 21 can correspondingly rotate under the action of the motor 2, the placing cylinder 3 is further driven to correspondingly rotate, the separating cylinder 4 and the centrifugal cylinder 5 are further driven to correspondingly rotate, the centrifugal cylinder 5 can perform centrifugal operation, the culture solution containing interleukin can be separated through the water permeable net 51, the culture solution can flow into the separating cylinder 4 through the water permeable net 51, the interleukin solution is still in the centrifugal cylinder 5, after the extraction and separation operation is finished, the user can turn off the motor 2, the centrifugal cylinder 5 can be rotated at the moment, the bottom end of the centrifugal cylinder 5 is separated from the threaded column 44, and the centrifugal cylinder 5 is disassembled, the interleukin solution can be conveniently taken out when the separation is finished, and then a user can rotate the four groups of fixing bolts 42, so that the bottom ends of the corresponding fixing bolts 42 are separated from the corresponding thread grooves, the separation cylinder 4 can be moved upwards at the moment, the iron rods 43 can be separated from the magnet blocks 34 when the separation cylinder 4 moves upwards, the separation cylinder 4 can be disassembled when the separation cylinder 4 completely moves out of the placing cylinder 3, so that the culture solution can be cleaned, otherwise, the separation cylinder 4 can be installed, the annular rubber strips 38 can play a role in buffering and supporting the separation cylinder 4, the sealing rings 37 can seal the inside of the through grooves 36 on one hand, and the iron rods 43 can be limited on the other hand;
when placing platform 21 and rotating, two sets of extension rods 22 can carry out corresponding rotation in the inside of ring channel 11, two sets of balls 23 can carry out corresponding rotation in the corresponding one side inner wall of ring channel 11, two sets of spacing grooves 12 are the annular setting, the event corresponds gag lever post 24 and can carry out corresponding rotation in the inside that corresponds spacing groove 12 respectively, thereby it is more stable when making to place platform 21 and rotate, when placing a section of thick bamboo 3 and rotate, annular slide rail 31 can carry out corresponding slip in the corresponding side surface of four group's sliders 13 thereupon, can make more stable and difficult emergence when placing a section of thick bamboo 3's rotation through the setting up of four group's sliders 13 and annular slide rail 31 and rock.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides an interleukin draws inclusion body separator that can ration, includes barrel (1), its characterized in that: the inner wall of the barrel body (1) is provided with an annular groove (11) by the middle position, the inner wall of the barrel body (1) is provided with limit grooves (12) by the top end and the bottom end of the annular groove (11), the inner wall of the barrel body (1) is provided with four groups of sliders (13) by the top end position, the inner wall of the bottom end of the barrel body (1) is provided with a motor (2) by the middle position, the output end of the motor (2) is connected with a placing table (21) in a transmission manner, extension rods (22) are arranged on two sides of the placing table (21), balls (23) are arranged at one ends of the two groups of extension rods (22), limit rods (24) are arranged on the top end and the bottom end of the two groups of extension rods (22), the top end of the placing table (21) is provided with a placing barrel (3), and two groups of annular slide rails (31) are arranged on the outer surface of the placing barrel (3), the bottom end inner wall of the placing barrel (3) is provided with a mounting seat (32), the inside of the mounting seat (32) is provided with a mounting groove (33), the bottom end inner wall of the mounting groove (33) is provided with a magnet block (34), the inside of the placing barrel (3) is provided with a baffle plate (35) by the bottom end position, the inside of the baffle plate (35) is provided with a through groove (36) by the middle position, the inside of the through groove (36) is provided with a sealing ring (37), the top end of the through groove (36) is provided with an annular rubber strip (38), the top surface of the magnet block (34) is fixedly attached to the bottom end of an iron rod (43), the top end of the iron rod (43) is fixed to the bottom end of the separating barrel (4), the outer surface of the separating barrel (4) is provided with four groups of fixing plates (41) by the top end position, the four groups of fixing plates (41) are movably screwed with fixing bolts (42), the bottom inner wall of the separating cylinder (4) is provided with a threaded column (44) near the middle, the outer surface of the threaded column (44) is movably screwed with a centrifugal cylinder (5), and the inner wall of the centrifugal cylinder (5) is provided with a water permeable net (51) near the bottom.
2. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the sizes of the two groups of balls (23) are equal, and the outer surfaces of the two groups of balls (23) are attached to the inner wall of the corresponding side of the annular groove (11).
3. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the number of the limiting rods (24) is four, the four limiting rods (24) are equal in size, and one ends of the four limiting rods (24) are respectively located in the corresponding limiting grooves (12).
4. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the four groups of sliding blocks (13) are equal in size, the four groups of sliding blocks (13) are arranged in a central symmetry mode by taking the center point of the cylinder body (1) as a central point, and the outer surfaces of the corresponding sides of the four groups of sliding blocks (13) are in sliding connection with the annular sliding rail (31).
5. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the four groups of fixing plates (41) are equal in size, and the four groups of fixing plates (41) are arranged in a central symmetry mode by taking the central point of the separating cylinder (4) as a central point.
6. The device for separating inclusion bodies according to claim 1, wherein the device comprises: threaded grooves are formed in the top end of the placing barrel (3) close to the positions corresponding to the fixing bolts (42), and the bottom ends of the four groups of fixing bolts (42) are movably connected with the corresponding threaded grooves in a threaded mode respectively.
7. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the internal diameter size of sealing washer (37) and the external diameter size looks adaptation of iron system pole (43), the internal diameter size of mounting groove (33) and the external diameter size looks adaptation of iron system pole (43).
8. The device for separating inclusion bodies according to claim 1, wherein the device comprises: the two groups of extension rods (22) are equal in size, and the thickness of the two groups of extension rods (22) is matched with the distance between the inner wall of the top end of the annular groove (11) and the inner wall of the bottom end of the annular groove.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111680972.6A CN114308405A (en) | 2021-12-31 | 2021-12-31 | Interleukin is extracted inclusion body separator that can ration ratio |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111680972.6A CN114308405A (en) | 2021-12-31 | 2021-12-31 | Interleukin is extracted inclusion body separator that can ration ratio |
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| CN114308405A true CN114308405A (en) | 2022-04-12 |
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| CN202111680972.6A Pending CN114308405A (en) | 2021-12-31 | 2021-12-31 | Interleukin is extracted inclusion body separator that can ration ratio |
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| CN213650752U (en) * | 2020-09-21 | 2021-07-09 | 陆杏 | Peculiar smell centrifugal processing device is prevented to kitchen garbage |
| CN214390996U (en) * | 2020-12-30 | 2021-10-15 | 四川瑞达新材料科技有限公司 | Nanometer material screening equipment |
| CN215023060U (en) * | 2020-11-10 | 2021-12-07 | 王珊 | A drainage device is used in nursing for ICU severe medical science branch of academic or vocational study |
| CN215313166U (en) * | 2021-06-28 | 2021-12-28 | 高邮市宇航化工机械厂 | Chemical waste residue solid-liquid separation device |
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2021
- 2021-12-31 CN CN202111680972.6A patent/CN114308405A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181698A (en) * | 2006-11-17 | 2008-05-21 | 岛产业株式会社 | Garbage treatment device and container for garbage treatment |
| CN204680102U (en) * | 2015-04-23 | 2015-09-30 | 赵清浩 | A kind of intelligence sells milk machine |
| CN210473253U (en) * | 2019-07-25 | 2020-05-08 | 淮安市匹克斯生物科技有限公司 | Separation and purification device for alkaloid extraction |
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| CN213650752U (en) * | 2020-09-21 | 2021-07-09 | 陆杏 | Peculiar smell centrifugal processing device is prevented to kitchen garbage |
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| CN215313166U (en) * | 2021-06-28 | 2021-12-28 | 高邮市宇航化工机械厂 | Chemical waste residue solid-liquid separation device |
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