CN208234818U - Novel antibodies purification devices - Google Patents
Novel antibodies purification devices Download PDFInfo
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- CN208234818U CN208234818U CN201820519158.3U CN201820519158U CN208234818U CN 208234818 U CN208234818 U CN 208234818U CN 201820519158 U CN201820519158 U CN 201820519158U CN 208234818 U CN208234818 U CN 208234818U
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Abstract
The utility model discloses a kind of novel antibodies purification devices, it include: fixing seat, the upper end is fixedly installed Miniature liquid pump, the fixing seat lower end is provided with multiple-way valve, bearing is provided at the fixing seat center, it is connected between the Miniature liquid pump and the multiple-way valve by drainage tube, the drainage tube and the bearing are rotatablely connected；Purification column, it is arranged in the multiple-way valve lower end, the purification column upper end is connected to by catheter with the multiple-way valve, the purification column includes purification column ontology and is from top to bottom successively set on the intrinsic albumin A of the purification column/G affinity column, ion exchange column and molecular sieve chromatography, the ion exchange column is connected to the albumin A/G affinity column and the molecular sieve chromatography respectively by pipeline, filter membrane is respectively arranged in the pipeline, the aperture of the filter membrane is sequentially reduced from top to bottom.
Description
Technical field
The utility model relates to field of biotechnology, in particular to a kind of novel antibodies purification devices.
Background technique
Antibody is a kind of special protein molecule, by the drug of reagent, treatment disease as in-vitro diagnosis, immune parent
With the aglucon of chromatography etc..It has a wide range of applications in life science, biotechnology and medical domain.Especially antibody is made
For the core reagent of various immunoassays, the sensitivity of immunoassay results, specificity are played a crucial role.Preparation
High specific, high-titer antibody be the basis of immunological technique, but no matter using the antibody of which kind of technology of preparing require into
Row purifying.
Antibody product is mainly derived from plasma composition or animal cell culture supernatant of donor and all kinds of animals etc.
In biological raw material.The purification devices mainly taken at present have column chromatography device, ammonium sulfate precipitation device, these devices are all deposited
In some defects: column chromatography specificity is good, but device and cumbersome, it is difficult to carry out the high throughput of research and development property scale quickly
Purifying；Ammonium sulfate precipitation purification specificity is poor, and purification process is cumbersome and time-consuming.Therefore, it is necessary to a kind of not only simple, but also can be high
The novel antibodies purification devices of antibody purification are rapidly completed in flux.
Utility model content
One purpose of the utility model is to solve at least the above problems, and provide the advantages of at least will be described later.
The utility model is to provide a kind of novel antibodies purification devices there are one purpose, can to avoid external environment and
Artificial disturbing factor can be improved antibody purification efficiency, and purification effect is good, practical.
In order to realize these purposes and other advantages according to the present utility model, a kind of novel antibodies purifying dress is provided
It sets, comprising:
Fixing seat, the upper end are fixedly installed Miniature liquid pump, and the fixing seat lower end is provided with multiple-way valve, the fixation
Seat center at be provided with bearing, be connected between the Miniature liquid pump and the multiple-way valve by drainage tube, the drainage tube and
The bearing rotation connection；
Purification column, setting are connected in the multiple-way valve lower end, the purification column upper end by catheter and the multiple-way valve
Logical, the purification column includes purification column ontology and is from top to bottom successively set on the intrinsic albumin A of the purification column/G parent
With chromatographic column, ion exchange column and molecular sieve chromatography, it is close with the albumin A/G respectively that the ion exchange column passes through pipeline
Be connected to chromatographic column with the molecular sieve chromatography, be respectively arranged with filter membrane in the pipeline, the aperture of the filter membrane from
It is sequentially reduced under.
Preferably, purification column ontology lower end is provided with liquid outlet, is provided with flow control valve on the liquid outlet,
The purification column body bottom portion is taper.
It preferably, further include grillage, the grillage includes bottom plate and the baffle for being fixed at the bottom plate two sides,
20-30 group is provided with for placing the first jack of the purification column, first jack and institute along the bottom plate uniform intervals
Purification column body bottom portion size fit is stated, the lower surface of base plate is provided with multiple support columns, and the support column includes telescopic rod
And screw rod, the screw rod connect the telescopic rod and the bottom plate, the lower surface of base plate is equipped with the spiral shell being adapted to the screw rod
Line bottom outlet.
Preferably, at least two rows of sockets being arranged above and below are provided on the baffle inner sidewall, in two baffles
Partition is set between the slot of same row, be respectively arranged at left and right sides of the partition be adapted to the slot it is convex
Portion, and the second jack being adapted to the purification column cylinder sizes is provided on the partition, the first jack diameter dimension
Less than the second jack diameter dimension, so that the purification column can be fixed on the grillage.
Preferably, vibrating motor is additionally provided on the grillage.
Preferably, purification column lower end side is equipped with backwash tube, and the purification column includes internal layer and outer layer, institute
It states and forms hollow interlayer between internal layer and the outer layer, interlayer side lower end is equipped with water inlet, the interlayer other side
Upper end is equipped with water outlet.
Preferably, degreasing sliver is provided between the purification column.
The utility model is include at least the following beneficial effects:
Firstly, being connected to purification column with multiple-way valve by setting Miniature liquid pump, human factor and environment can not only be avoided
Pollution of the factor to purification process, and can be realized disposable injection of antibodies and purified to multiple groups purification column, it being capable of high pass
Amount completes antibody purification procedures, substantially increases purification efficiency.
Secondly, the albumin A being from top to bottom successively set in purification column/G affinity column, ion exchange column and molecule
Sieve chromatography column can effectively antagonize body and carry out separating-purifying, and albumin A/G affinity column is to utilize albumin A and the affine layer of Protein G
Analysis is purified；Ion exchange column is to have isoelectric point according to protein, protein is different to the separating-purifying of antibody
Under the conditions of pH, charged state is also different, thus to antibody separating-purifying；Molecular sieve chromatography is to utilize to have certain pore size range
Porous gel as stationary phase, each component in mixture is separated by molecular size, reaches the mesh of isolated antibody
's.
Finally, by being connected to the albumin A/G affinity column and the molecular sieve chromatography in ion exchange column
Filter membrane is set in pipeline, can further impurity screening, antibody purification ensures antibody quality, stabilization of antibodies purification process.This reality
It is simple with novel antibodies purification devices structure, it is easy to operate, it can be to avoid environment and artificial disturbing factor, Neng Gouti
High antibody purification efficiency, and purification effect is good, it is practical.
The further advantage, target and feature of the utility model will be partially reflected by the following instructions, and part will also pass through
Research and practice to the utility model and be understood by the person skilled in the art.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of novel antibodies purification devices described in the utility model；
Fig. 2 is the purification column structural schematic diagram of novel antibodies purification devices described in the utility model；
Fig. 3 is the framed plate structure schematic diagram of novel antibodies purification devices described in the utility model.
Specific embodiment
The following describes the utility model in further detail with reference to the accompanying drawings, to enable those skilled in the art referring to explanation
Book text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
As shown in Figure 1, Figure 2 and Figure 3, the utility model provides a kind of novel antibodies purification devices, comprising:
Fixing seat 10, the upper end are fixedly installed Miniature liquid pump 11, and 10 lower end of fixing seat is provided with multiple-way valve 12,
It is provided with bearing 13 at 10 center of fixing seat, passes through drainage tube 14 between the Miniature liquid pump 11 and the multiple-way valve 12
Connection, the drainage tube 14 are rotatablely connected with the bearing 13；
Purification column, setting pass through catheter 25 and the multi-pass in 12 lower end of multiple-way valve, the purification column upper end
Valve 12 is connected to, and the purification column includes purification column ontology 15 and is from top to bottom successively set in the purification column ontology 15
Albumin A/G affinity column 16, ion exchange column 17 and molecular sieve chromatography 18, the ion exchange column 17 pass through pipeline point
It is not connected to the albumin A/G affinity column 16 and the molecular sieve chromatography 18, filtering is respectively arranged in the pipeline
The aperture of film, the filter membrane is sequentially reduced from top to bottom.
In the above scheme, Miniature liquid pump 11 refer to have one-in-and-one-out pump mouth, discharge outlet each one, and into
Vacuum or negative pressure can be persistently formed at mouthful；Larger output pressure is formed at discharge outlet；Working media is water or liquid；It is small in size
A kind of skilful instrument.With multiple-way valve 12 be connected to purification column by setting Miniature liquid pump 11, can not only avoid human factor with
Pollution of the environmental factor to purification process, and can be realized disposable injection of antibodies and purified to multiple groups purification column, it can
High throughput completes antibody purification procedures, substantially increases purification efficiency.
Albumin A/G affinity column 16, ion exchange column 17 and molecular sieve chromatography 18 are from top to bottom successively set on
In purification column ontology 15, albumin A/G affinity column 16 is purified using albumin A and protein g affinity chromatography, golden yellow Portugal
Grape pneumococcal proteins A is currently the main medium of monoclonal antibody purifying, in antibody purification procedures due to its outstanding antibody binding capacity
In, using the staphylococcus aureus protein A being crosslinked with inertia chromatography media as aglucon, the Fc of albumin A and immunoglobulin G
Structural domain, which combines, carries out affinity chromatography, to get to the purpose of antibody purification, Protein G passes through the nonimmune machine similar to albumin A
System is combined with the Fc of antibody section, and albumin A and Protein G are different to the antibody compatibility of separate sources, mixed using albumin A and Protein G
The chromatographic column of conjunction can reach better broad spectrum activity；Ion exchange column 17 to the separating-purifying of antibody, is had according to protein
Isoelectric point, protein are under the conditions of different pH, and charged state is also different, and anion exchange matrix, which combines, has negative electrical charge
Protein, cation exchange matrix combine with positive charge protein, to reach the separating-purifying to antibody；Molecular sieve
Chromatographic column 18 be using having the porous gel of certain pore size range as stationary phase, to each component in mixture by molecular size into
Row separation, achievees the purpose that isolated antibody；By in ion exchange column 17 and the albumin A/G affinity column 16 and institute
State molecular sieve chromatography 18 connection pipeline in be arranged filter membrane, can further impurity screening, antibody purification, ensure antibody product
Matter, stabilization of antibodies purification process.The utility model antibody purification apparatus structure is simple, easy to operate, can be to avoid environment
And artificial disturbing factor, it can be improved antibody purification efficiency, and purification effect is good, it is practical.
In one preferred embodiment, 15 lower end of purification column ontology is provided with liquid outlet, and stream is provided on the liquid outlet
Adjustable valve 24,15 bottom of purification column ontology are taper.
In the above scheme, the antibody in 15 lower end of purification column ontology setting liquid outlet for extraction purification is going out
Flow control valve 24 is arranged on liquid mouth to be avoided causing due to taking out misoperation convenient for adjusting the flow that antibody flows out liquid outlet
Antibody waste, the purification column body bottom portion, which is set as taper, can facilitate the extraction of antibody.
It further include grillage 19 in one preferred embodiment, the grillage 19 is including bottom plate 26 and is fixed at the bottom
The baffle 27 of 26 two sides of plate is provided with 20-30 group along 26 uniform intervals of bottom plate and inserts for placing the first of the purification column
Hole 31, first jack 31 are adapted to 15 bottom size of purification column ontology, and 26 lower surface of bottom plate is provided with multiple
Support column 28, the support column 28 include telescopic rod and screw rod, and the screw rod connects the telescopic rod and the bottom plate 26, described
26 lower surface of bottom plate is equipped with the thread Pilot hole being adapted to the screw rod.
In the above scheme, it is equidistantly positioned 20-30 group jack along the bottom plate 26, for fixing purifying described in multiple groups
Column realizes Isolation multiple groups antibody.28 bottom of support column is the screw rod to match with spiral bottom outlet, utilizes screw rod and screw thread bottom
The screw thread in hole is connected, and the connection of support column 28 Yu bottom plate 26 is realized with this, and under dismantling support column 28 can from bottom plate 26
Come, convenient for being stored to experimental apparatus.Setting support column 28 is used to support the grillage 19, and support column 28 is set as telescopic rod
Conveniently adjusted 28 height of support column, convenient for protection grillage 19 and purification column.
In one preferred embodiment, at least two rows of sockets 30 being arranged above and below are provided on 27 inner sidewall of baffle, two
A baffle 27, which is located between the slot 30 with row, is arranged partition 29, be respectively arranged at left and right sides of the partition 29 with
The protrusion that the slot 30 is adapted to, and the second jack being adapted to the purification column cylinder sizes is provided on the partition 29
32,31 diameter dimension of the first jack is less than the second jack diameter dimension 32, so that the purification column can be fixed on
On the grillage 19.
In the above scheme, the height of partition 29 can accordingly be adjusted according to the length of purification column, therefore adapt to put
The purification column for setting a variety of different sizes improves the practicability of utility model device.The protrusion insertion of 29 two sides of partition is inserted
In slot 30, to realize that partition 29 is fixed between two baffles 27.32 quantity of the second jack and bottom plate 26 on partition 29
On the quantity of the first jack 31 be consistent, the diameter of the first jack 31 can make purification column less than the diameter of the second jack 32
It is inserted into partition 29 and is fixed on bottom plate 26.
In one preferred embodiment, vibrating motor is additionally provided on the grillage 19.
In the above scheme, by the way that vibrating motor is arranged on the grillage 19, vibrating motor is opened, purification column can be made
At ringing effect, purification column is set to reach more effectively analysis purification effect by vibrating effect.
In one preferred embodiment, purification column lower end side is equipped with backwash tube 21, and the purification column includes internal layer
And outer layer, hollow interlayer is formed between the internal layer and the outer layer, interlayer side lower end is equipped with water inlet 22, described
Interlayer other side upper end is equipped with water outlet 23.
In the above scheme, by that can be backwashed to filter membrane in purification column lower end side installation backwash tube 21,
Achieve the purpose that remove the impurity being trapped on filter membrane, makes filter membrane that can restore filtering function in a short time, extend pure
The service life for changing column, enhances the practicability of purification column.
In one preferred embodiment, degreasing sliver is provided between the purification column.
In the above scheme, by between the purification column be arranged degreasing sliver, can effectively prevent the purification column it
Between cross-infection, ensured the quality of antibody purification.
It is not only in the description and the implementation although the embodiments of the present invention have been disclosed as above
Listed utilization, it can be applied to various fields suitable for the present invention completely, for those skilled in the art,
Other modifications may be easily implemented, therefore without departing from the general concept defined in the claims and the equivalent scope, this reality
It is not limited to specific details and legend shown and described herein with novel.
Claims (7)
1. a kind of novel antibodies purification devices characterized by comprising
Fixing seat, the upper end are fixedly installed Miniature liquid pump, and the fixing seat lower end is provided with multiple-way valve, in the fixing seat
Be provided with bearing at the heart, be connected between the Miniature liquid pump and the multiple-way valve by drainage tube, the drainage tube with it is described
Bearing rotation connection；
Purification column, setting are connected to by catheter with the multiple-way valve in the multiple-way valve lower end, the purification column upper end, institute
Purification column is stated to include purification column ontology and be from top to bottom successively set on the intrinsic albumin A of the purification column/G affinity chromatography
Column, ion exchange column and molecular sieve chromatography, the ion exchange column by pipeline respectively with the albumin A/G affinity chromatography
Column is connected to the molecular sieve chromatography, filter membrane is respectively arranged in the pipeline, the aperture of the filter membrane is from top to bottom
It is sequentially reduced.
2. novel antibodies purification devices as described in claim 1, which is characterized in that purification column ontology lower end is provided with out
Liquid mouth, flow control valve is provided on the liquid outlet, and the purification column body bottom portion is taper.
3. novel antibodies purification devices as described in claim 1, which is characterized in that further include grillage, the grillage includes bottom
Plate and the baffle for being fixed at the bottom plate two sides are provided with 20-30 group for placing along the bottom plate uniform intervals
The first jack of purification column, first jack and the purification column body bottom portion size fit are stated, the lower surface of base plate is set
Multiple support columns are equipped with, the support column includes telescopic rod and screw rod, and the screw rod connects the telescopic rod and the bottom plate, institute
It states lower surface of base plate and is equipped with the thread Pilot hole being adapted to the screw rod.
4. novel antibodies purification devices as claimed in claim 3, which is characterized in that be provided on the baffle inner sidewall up and down
At least two rows of sockets of arrangement are located between the slot with row in two baffles and partition, the partition or so are arranged
Two sides are respectively arranged with the protrusion being adapted to the slot, and are provided on the partition and are adapted to the purification column cylinder sizes
The second jack, the first jack diameter dimension be less than the second jack diameter dimension so that the purification column can be consolidated
It is scheduled on the grillage.
5. novel antibodies purification devices as claimed in claim 3, which is characterized in that be additionally provided with vibration electricity on the grillage
Machine.
6. novel antibodies purification devices as described in claim 1, which is characterized in that purification column lower end side is equipped with instead
Flushing pipe, the purification column include internal layer and outer layer, and hollow interlayer, the interlayer are formed between the internal layer and the outer layer
Side lower end is equipped with water inlet, and interlayer other side upper end is equipped with water outlet.
7. novel antibodies purification devices as described in claim 1, which is characterized in that be provided with absorbent cotton between the purification column
Item.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820519158.3U CN208234818U (en) | 2018-04-12 | 2018-04-12 | Novel antibodies purification devices |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820519158.3U CN208234818U (en) | 2018-04-12 | 2018-04-12 | Novel antibodies purification devices |
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| CN208234818U true CN208234818U (en) | 2018-12-14 |
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| CN201820519158.3U Active CN208234818U (en) | 2018-04-12 | 2018-04-12 | Novel antibodies purification devices |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109320603A (en) * | 2018-12-17 | 2019-02-12 | 杭州奕安济世生物药业有限公司 | A kind of system and method for serialization purification antibody |
| CN110981954A (en) * | 2020-01-23 | 2020-04-10 | 中科宝承生物医学科技有限公司 | Antibody purification equipment and operation method thereof |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
-
2018
- 2018-04-12 CN CN201820519158.3U patent/CN208234818U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109320603A (en) * | 2018-12-17 | 2019-02-12 | 杭州奕安济世生物药业有限公司 | A kind of system and method for serialization purification antibody |
| CN110981954A (en) * | 2020-01-23 | 2020-04-10 | 中科宝承生物医学科技有限公司 | Antibody purification equipment and operation method thereof |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
| CN113717837B (en) * | 2021-08-13 | 2023-10-03 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification column |
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