CN217868799U

CN217868799U - A extraction element for stem cell

Info

Publication number: CN217868799U
Application number: CN202221968730.7U
Authority: CN
Inventors: 章万古; 王冉冉
Original assignee: Guanhao Precision Hangzhou Biotechnology Co ltd
Current assignee: Guanhao Precision Hangzhou Biotechnology Co ltd
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2022-11-22
Anticipated expiration: 2032-07-28

Abstract

The utility model relates to a technical field is mentioned to the cell, especially relates to an extraction element for stem cell, comprising a base plate, the top one end of bottom plate is fixed with fixed frame, and fixed frame internal rotation is connected with the threaded rod, the threaded rod spiro union has the mount, be provided with adjustment mechanism on the mount, and adjustment mechanism clamp has the mechanism of drawing, adjustment mechanism includes the I-shaped piece, is fixed in the pipe strap of I-shaped piece one side outer wall, pegs graft the movable rod in the I-shaped piece. The utility model discloses a rotation threaded rod drives the mount and reciprocates, drives by the mount again and draws the mechanism and reciprocate, can keep stronger stability at the in-process that removes, simultaneously, drives the piston through rotating thin screw rod and reciprocates in drawing a section of thick bamboo, accomplishes the extraction to the stem cell separation layer, can keep stronger stability equally, can avoid the problem appearance of handheld shake, is convenient for carry out the work of drawing of stem cell.

Description

A extraction element for stem cell

Technical Field

The utility model relates to a cell extraction technical field especially relates to an extraction element for stem cell.

Background

At present, the methods for extracting and separating the mesenchymal stem cells in the laboratory mainly comprise a density gradient centrifugation method, an adherence screening method (whole bone marrow adherence method), a cell surface molecular marker sorting method, a cell screening method and the like, and the most common method is to combine the density gradient centrifugation method and the adherence screening method. In the process of density gradient centrifugation, a liquid column in a test tube is divided into 4 layers after the marrow liquid is centrifuged in the test tube, the first layer is a culture liquid layer, the second layer is a mononuclear cell layer (containing bone marrow mesenchymal stem cells needed by people), the third layer is a lymphocyte separation liquid layer, and the fourth layer is a red blood cell and polynuclear cell layer.

In the prior art, an extraction syringe is usually inserted into a cell layer to be extracted for extraction, but the following defects exist in the actual operation process:

in the process of extraction, the hand-held extraction syringe is difficult to keep stable, shaking occurs, and extraction of the cell layer and the solution layer close to each other is difficult.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing an extraction device for stem cells.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides an extraction element for stem cell, includes the bottom plate, the top one end of bottom plate is fixed with fixed frame, and the fixed frame internal rotation is connected with the threaded rod, the threaded rod spiro union has the mount, be provided with adjustment mechanism on the mount, and adjustment mechanism clamp has the mechanism of drawing, adjustment mechanism includes the I-shaped piece, be fixed in the pipe strap of I-shaped piece one side outer wall, peg graft the movable rod in the I-shaped piece and be fixed in the stopper at movable rod top, draw the mechanism including draw a section of thick bamboo, peg graft and be fixed in the needle tubing that draws a section of thick bamboo bottom, peg graft in the cover that draws a section of thick bamboo top, the thin screw rod of spiro union in cover top and rotate the piston of connecting in thin screw rod bottom.

Preferably, the top of fixed frame is provided with the runner handle, and the runner handle is connected fixedly with the top of threaded rod.

Preferably, one end of the outer wall of each of the two sides of the fixing frame is provided with a sliding groove, and the I-shaped block and the sliding groove form sliding fit.

Preferably, the top of the I-shaped block is provided with a socket, and the movable rod and the socket form sliding fit.

Preferably, the both sides of mount top one end all are equipped with the rack, and the bottom both ends of stopper seted up with rack intermeshing's tooth's socket.

Preferably, the extraction cylinder forms an interference fit with the pipe clamp, and the extraction cylinder forms an interference fit with the cylinder cover.

The utility model has the advantages that:

drive the mount through rotating the threaded rod and reciprocate, drive by the mount again and draw the mechanism and reciprocate, can keep stronger stability at the in-process that removes, simultaneously, drive the piston through rotating thin screw rod and reciprocate in drawing a section of thick bamboo, accomplish the extraction to the stem cell separation layer, can keep stronger stability equally, can avoid the problem of handheld shake to appear, be convenient for carry out the work of drawing of stem cell.

Drawings

Fig. 1 is a schematic diagram of the overall structure of an extraction device for stem cells according to the present invention;

fig. 2 is a schematic structural diagram of an adjusting mechanism of an extraction device for stem cells according to the present invention;

fig. 3 is a schematic structural diagram of an extraction mechanism of the extraction device for stem cells according to the present invention.

In the figure: 1. a base plate; 2. a fixing frame; 3. a threaded rod; 4. a fixed mount; 5. an adjustment mechanism; 6. an extraction mechanism; 7. a shaping block; 8. a pipe clamp; 9. a movable rod; 10. a limiting block; 11. an extraction cylinder; 12. a needle tube; 13. a cylinder cover; 14. a fine screw; 15. a piston.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Example (b):

referring to fig. 1, an extraction device for stem cells comprises a bottom plate 1, wherein a fixed frame 2 is fixed at one end of the top of the bottom plate 1, a threaded rod 3 is connected in the fixed frame 2 in a rotating mode, a rotating wheel handle is arranged at the top of the fixed frame 2 and is fixedly connected with the top end of the threaded rod 3, the threaded rod 3 is in threaded connection with a fixed frame 4, an adjusting mechanism 5 is arranged on the fixed frame 4, the threaded rod 3 is rotated by rotating the rotating wheel handle, the height of the fixed frame 4 is lifted, the lifting speed is stable, the control is convenient, and the separated cell layer can be extracted conveniently;

referring to fig. 2, the adjusting mechanism 5 clamps the extracting mechanism 6, and the adjusting mechanism 5 comprises a die block 7, a pipe clamp 8 fixed on the outer wall of one side of the die block 7, a movable rod 9 inserted in the die block 7, and a limit block 10 fixed on the top of the movable rod 9;

the device comprises a fixing frame 4, a rack, a limiting block 10, a sliding groove, a sliding rod 9, racks, a tooth socket, a limiting block 10, a regulating mechanism 5 and a regulating mechanism 5, wherein the sliding groove is formed in one end of the outer wall of the two sides of the fixing frame 4, the I-shaped block 7 is in sliding fit with the sliding groove, the socket is formed in the top of the I-shaped block 7, the sliding rod 9 is in sliding fit with the socket, the racks are arranged on the two sides of one end of the top of the fixing frame 4, the tooth sockets meshed with the racks are formed in the two ends of the bottom of the limiting block 10, the tooth sockets at the bottom of the limiting block 10 are separated from the racks by lifting the limiting block 10 upwards, the I-shaped block 7 can slide freely, and the device can be suitable for separating bottles with different calibers by regulating the position of the regulating mechanism 5;

referring to fig. 3, the extraction mechanism 6 includes an extraction barrel 11, a needle tube 12 inserted and fixed at the bottom end of the extraction barrel 11, a barrel cover 13 inserted and fixed at the top end of the extraction barrel 11, a fine screw 14 screwed on the top of the barrel cover 13, and a piston 15 rotatably connected at the bottom end of the fine screw 14;

wherein, draw a section of thick bamboo 11 and pipe strap 8 and form interference fit, draw a section of thick bamboo 11 and cover 13 and form interference fit, through rotating thin screw 14, by the screw-thread fit between thin screw 14 and the cover 13, make thin screw 14 reciprocate, and then drive piston 15 and reciprocate in drawing a section of thick bamboo 11, when needle tubing 12 is located the cell layer that needs to draw, can draw out the stem cell through needle tubing 12.

The working principle is as follows:

during the use, mention stopper 10 upwards, make the tooth's socket and the rack of stopper 10 bottom break away from, make I type piece 7 slide in the spout, make needle tubing 12 can be located directly over the bottleneck, then fall stopper 10, stopper 10 and rack toothing, I type piece 7 can not slide freely this moment, then rotate the runner handle, make threaded rod 3 rotate, descend the height of mount 4, make needle tubing 12 insert the stem cell separation layer that needs to draw, rotate thin screw 14 subsequently, by the screw-thread fit between thin screw 14 and cover 13, make thin screw 14 draw a section of thick bamboo 11 again and move from bottom to top, and then drive piston 15 and carry out the rebound in drawing a section of thick bamboo 11, draw out the stem cell through needle tubing 12.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides an extraction element for stem cell, includes bottom plate (1), its characterized in that, the top one end of bottom plate (1) is fixed with fixed frame (2), and fixed frame (2) internal rotation is connected with threaded rod (3), threaded rod (3) spiro union has mount (4), be provided with adjustment mechanism (5) on mount (4), and adjustment mechanism (5) centre gripping has the mechanism (6) of drawing, adjustment mechanism (5) include worker's type piece (7), be fixed in pipe strap (8) of worker's type piece (7) one side outer wall, peg graft movable rod (9) in worker's type piece (7) and be fixed in stopper (10) at movable rod (9) top, draw mechanism (6) including drawing a section of thick bamboo (11), peg graft and be fixed in syringe (12) of drawing a section of thick bamboo (11) bottom, peg graft in section of thick bamboo lid (13) top of drawing a section of thick bamboo (11), thin screw rod (14) and the piston (15) of spiro union connection in thin screw rod (14) bottom of thin screw rod (14) of thin screw rod (11) top.

2. The extraction device for stem cells according to claim 1, characterized in that the top of the fixed frame (2) is provided with a turning wheel handle, and the turning wheel handle is fixedly connected with the top end of the threaded rod (3).

3. The extraction device for stem cells according to claim 1, wherein the fixing frame (4) has a sliding groove at one end of the outer wall at both sides, and the I-shaped block (7) forms a sliding fit with the sliding groove.

4. The extraction device for stem cells according to claim 1, wherein the top of the I-shaped block (7) is provided with a socket, and the movable rod (9) forms a sliding fit with the socket.

5. The extraction device for the stem cells, according to claim 1, characterized in that racks are arranged on both sides of one end of the top of the fixing frame (4), and tooth sockets meshed with the racks are arranged on both ends of the bottom of the limiting block (10).

6. An extraction device for stem cells according to claim 1, wherein the extraction cartridge (11) forms an interference fit with the cartridge (8) and the extraction cartridge (11) forms an interference fit with the cartridge cover (13).