CN114306167B - Whitening and moisturizing composition containing composite plant extract and preparation method thereof - Google Patents
Whitening and moisturizing composition containing composite plant extract and preparation method thereof Download PDFInfo
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Abstract
The application relates to a whitening and moisturizing composition containing a composite plant extract and a preparation method thereof, wherein the preparation method comprises the following steps: step one, uniformly mixing bearberry leaves, butcher's broom, strawberry fruits, shepherd's purse, glabrous licorice, aloe, butanediol and water, and carrying out dissolving and soaking for 30-35min to obtain a raw material mixture; step two, heating the raw material mixture to more than 100 ℃, performing reflux circulation, stopping heating after heating for 1.2-1.5h, and keeping the temperature for 30-35min; step three, cooling to 30-35 ℃, and filtering to obtain an intermediate mixture; and step four, adding the preservative and the auxiliary agent into the intermediate mixture, uniformly stirring at the constant temperature of 30-35 ℃, filtering, standing for 12 hours to allow the liquid to be layered, discharging supernatant liquid, and reserving lower-layer liquid to obtain the whitening and moisturizing composition containing the composite plant extract. The whitening and moisturizing composition containing the composite plant extract has a relatively outstanding whitening and moisturizing effect.
Description
Technical Field
The application relates to the field of cosmetics, in particular to a whitening and moisturizing composition containing a composite plant extract.
Background
With the severe changes of modern social environment, climate and the like, the harm of external environment to the skin of people is gradually enlarged, especially melasma, freckles, age spots and the like caused by excessive precipitation of melanin become one of the mainstream products of skin care cosmetics, but the common whitening products in the market at present often contain a lot of chemical substances which have certain damage to the skin. Although there are skin care products in the prior art which are added with plants or natural substances to improve the whitening effect, the skin care products have low natural substance content and only have weak auxiliary effect, so the whitening effect is also poor. In view of these problems, the inventors of the present invention considered that there is much room for improvement in the reduction of melanin and the improvement of whitening effects mainly by using natural substances such as plants.
Disclosure of Invention
The application provides a whitening and moisturizing composition containing a composite plant extract and a preparation method thereof, in order to improve the effect of combination of natural substances such as plants on whitening and obtaining a whitening composition mainly containing the plant extract.
In a first aspect, the preparation method of the whitening and moisturizing composition containing the composite plant extract adopts the following technical scheme:
a method for preparing a whitening and moisturizing composition containing compound plant extracts comprises the following steps:
step one, uniformly mixing bearberry leaves, butcher's broom, strawberry fruits, shepherd's purse, glabrous licorice, aloe, butanediol and water, and carrying out dissolving and soaking for 30-35min to obtain a raw material mixture;
step two, heating the raw material mixture to more than 100 ℃, performing reflux circulation, stopping heating after heating for 1.2-1.5h, and keeping the temperature for 30-35min;
step three, cooling to 30-35 ℃, and filtering to obtain an intermediate mixture;
adding a preservative and an auxiliary agent into the intermediate mixture, uniformly stirring at a constant temperature of 30-35 ℃, filtering, standing for 12 hours to allow liquid to be layered, discharging supernatant liquid, and reserving lower-layer liquid to obtain the whitening and moisturizing composition containing the composite plant extract;
the mass percentages of the components are as follows: 2-4wt% of bearberry leaves, 1-3wt% of Ruscus aculeatus, 1-3wt% of strawberry tree fruits, 1-3wt% of shepherd's purse, 2-4wt% of Glycyrrhiza glabra, 1-3wt% of aloe, 19-21wt% of butanediol, 0.5-1wt% of preservative, 0.02-0.04wt% of auxiliary agent and water which are added to 100wt%.
By adopting the technical scheme, under the auxiliary action of water and butanediol, active substances in the plant are dissolved in the water and the butanediol to obtain various plant extracts, wherein the arbutin leaf extract mainly contains arbutin which can effectively inhibit the activity of biological tyrosinase in the skin and block the formation of melanin; the camelina sativa extract has an effect of eliminating a plurality of free radicals, so that skin pigmentation is reduced, and the combined action of the two helps to improve the whitening and freckle removing effects of the composition; the Ruscus aculeatus extract can enhance skin cell metabolism, has activating effect on luciferase and aromatase, and has remarkable anti-aging effect; a large amount of phenolic compounds, especially flavone-3-ol, in the strawberry tree fruit extract have biological activities such as oxidation resistance, antibiosis and the like, and the two components act together to further contribute to improving the whitening and anti-aging effects; glycyrrhiza glabra extract can penetrate into skin and maintain high activity, and it can inhibit dopamine interconversion and DHICA oxidase activity, has oxygen free radical scavenging ability similar to SOD (superoxide dismutase), and has oxygen free radical resisting ability similar to vitamin E; the addition of the glycyrrhiza glabra extract is beneficial to relieving the inhibition effect between the bearberry leaf extract and the butcher's broom extract on the whitening effect, and further enhances the moisturizing and skin-brightening effects of the composition; the aloe extract in the composition further improves the effects of whitening, anti-wrinkle, moisturizing and brightening the skin of the composition.
The mixture prepared by the application is easier to be absorbed by the skin through the synergistic effect of natural components of all plants and the good moisturizing and permeability of butanediol, so that the skin is improved from multiple aspects, and the whitening and skin brightening effects are prominent.
Preferably, in the fourth step, the preparation method of the preservative comprises the following steps: 1,2 hexanediol and p-hydroxyacetophenone are mixed according to the mass ratio of (1-1.1): 1, mixing and heating to 60-85 ℃, and uniformly stirring to obtain the preservative.
1,2 hexanediol is a polyhydric alcohol with high antibacterial performance, and has extremely high hygroscopicity and water retention; the p-hydroxyacetophenone has safe and efficient preservative effect; the molecular weight of the 1,2 hexanediol is smaller, and the skin irritation effect is smaller, by adopting the technical scheme, the 1,2 hexanediol and the p-hydroxyacetophenone are mixed to prepare the preservative, and the addition of the preservative is not only favorable for improving the preservative and antibacterial effects of the composition, but also capable of improving the comfortable feeling and the absorption effect of the skin when the composition is used.
Preferably, 0.5wt% -2.1wt% of pawpaw is also added in the first step.
The papaya extract can be obtained by adopting the technical scheme, contains malic acid, tartaric acid, citric acid, saponin and flavonoid, has good compatibility with the strawberry tree fruit extract, can act together with the strawberry tree fruit extract, greatly improves the activity of skin cells, and further enhances the whitening and skin brightening effects of the composition.
Preferably, 0.8-3 wt% of hawthorn is also added in the first step.
The hawthorn extract can be obtained by adopting the technical scheme, contains hawthorn flavone, vitexin, crataegolic acid, hyperin, epicatechin and the like, has a good inhibition effect on tyrosinase and metalloprotease, has a clearing effect on hydroxyl free radicals and superoxide radicals, has a strong activation effect on luciferase, and has a promoting effect on the synthesis of ceramide, so that the hawthorn extract can be cooperatively matched with each plant extract component in the composition, and further plays a gain role in various aspects of whitening, wrinkle resistance, speckle reduction, antibiosis, moisture retention and the like of the composition, and the overall performance of the composition is further improved.
Preferably, 1 to 1.5 weight percent of pawpaw and 1.6 to 2.4 weight percent of hawthorn are also added in the first step.
By adopting the technical scheme, the prepared composition contains the papaya extract, the hawthorn extract and the Ruscus aculeatus extract, the papaya extract, the hawthorn extract and the Ruscus aculeatus extract are mixed according to a certain proportion, and a certain synergistic effect can be generated among the papaya extract, the hawthorn extract and the Ruscus aculeatus extract, so that the activity of skin cells is greatly improved, the absorption effect of skin is promoted, the water content of skin can be kept in a sufficient state for a long time, and the whitening and brightening effects of the composition on skin are further improved.
Preferably, 2-3wt% of chamomile flowers are also added in the first step.
The chamomile flower extract obtained by adopting the technical scheme contains flavonoid compounds, amino acid and other components, can act together with the aloe extract, and has a good moisturizing effect.
Preferably, the auxiliary agent is disodium EDTA.
Through adopting above-mentioned technical scheme, through adding EDTA disodium in the composition, EDTA disodium can play the effect of chelating agent, and then helps promoting associativity and compatibility between components such as plant extract and solvent in the composition, and then makes evenly to cooperate between the inside component of composition, and the effect of composition promotes better.
In a second aspect, the present application provides a whitening and moisturizing composition containing a composite plant extract, which adopts the following technical scheme:
a whitening and moisturizing composition containing compound plant extracts is prepared by adopting the preparation method of the whitening and moisturizing composition containing the compound plant extracts.
In summary, the present application includes at least one of the following beneficial technical effects:
1. the composition prepared in the application contains various plant extracts, and the bearberry leaf extract and the camelina sativa extract have combined action, so that the whitening and freckle removing effects of the composition are improved; the Ruscus aculeatus extract and the strawberry fruit extract act together, so that the whitening and anti-aging effects are improved; the glycyrrhiza glabra extract can penetrate into the skin and keep high activity, is beneficial to relieving the inhibiting effect between the bearberry leaf extract and the ruscus aculeatus extract on whitening effect, and further enhances the moisturizing effect of the composition; aloe extract has effects of keeping moisture and brightening skin; through various synergistic cooperation among the plant extracts, the composition has more outstanding effects of whitening, resisting aging, preserving moisture and brightening skin;
2. the composition also comprises a hawthorn extract, wherein the hawthorn extract contains hawthorn flavone, vitexin, crataegolic acid, hyperin, epicatechin and the like, has a good inhibition effect on tyrosinase and metalloproteinase, has a clearing effect on hydroxyl radicals and superoxide radicals, has a strong activation effect on luciferase, and has a promotion effect on synthesis of ceramide, so that the hawthorn extract can be cooperatively matched with each plant extract component in the composition, and further plays a gain role in various aspects of whitening, wrinkle resistance, speckle reduction, antibiosis, moisture preservation and the like of the composition, and the overall performance of the composition is further improved; 3. according to the composition, pawpaw extract, hawthorn extract and Ruscus aculeatus extract in the composition can possibly generate a certain synergistic effect after the pawpaw extract, the hawthorn extract and the Ruscus aculeatus extract are mixed according to a certain proportion, so that the activity of skin cells is greatly improved, the absorption effect of skin is promoted, the water content of the skin can be kept in a sufficient state for a long time, and the whitening and brightening effects of the composition on the skin are further improved.
Detailed Description
The present application will be described in further detail with reference to examples.
Examples
Example 1
The embodiment discloses a preparation method of a whitening and moisturizing composition containing compound plant extracts, which comprises the following steps:
step one, mixing 2kg of bearberry leaves, 1kg of butea frondosa, 1kg of strawberry tree fruits, 1kg of shepherd's purse, 2kg of glabrous licorice, 1kg of aloe, 19kg of butanediol and 72.48kg of water, adding the mixture into an emulsifying pot, stirring for 5min at normal temperature and at the rotating speed of 30r/min, and then dissolving and soaking for 30min to obtain a raw material mixture;
step two, carrying out reflux circulation at a constant temperature of 100 ℃, stopping heating after heating for 1.2h, and keeping the temperature for 30min;
step three, after cooling to 30 ℃, filtering plant residues to obtain an intermediate mixture;
adding 0.5kg of preservative and 0.02kg of auxiliary agent into the intermediate mixture, stirring at constant temperature of 30 ℃ for 30min, filtering by adopting a 100nm nano-film, standing for 12h after filtering to enable liquid to be layered, discharging supernatant liquor through a water suction pipe to obtain lower-layer liquid, namely the whitening and moisturizing composition containing the composite plant extract, canning and sealing the composition; in the embodiment, the preservative is benzyl alcohol and the auxiliary agent is propylene glycol.
Example 2
The embodiment is different from the embodiment 1 in that: the dosage of each raw material component and the temperature in the preparation process are different, and the method specifically comprises the following steps: step one, mixing 4kg of bearberry leaves, 3kg of butea frondosa, 3kg of strawberry tree fruits, 3kg of shepherd's purse, 4kg of glabrous licorice, 3kg of aloe, 21kg of butanediol and 57.96kg of water, adding into an emulsifying pot, stirring for 5min at normal temperature and under the condition that the rotating speed is 30r/min, and then dissolving and soaking for 35min; step two, stopping heating after heating for 1.5 hours; step three, cooling to 35 ℃; and step four, adding 1kg of benzyl alcohol and 0.04kg of propylene glycol, and stirring at the constant temperature of 35 ℃.
Example 3
The embodiment is different from the embodiment 1 in that: the dosage of each raw material component and the temperature in the preparation process are different, and the method specifically comprises the following steps: step one, mixing 3kg of bearberry leaves, 2kg of butea frondosa, 2kg of strawberry tree fruits, 2kg of shepherd's purse, 3kg of glabrous licorice, 2kg of aloe, 20kg of butanediol and 64.96kg of water and adding the mixture into an emulsifying pot; step two, stopping heating after heating for 1.5 hours; step three, cooling to 30 ℃; and step four, adding 1kg of benzyl alcohol and 0.04kg of propylene glycol, and stirring at the constant temperature of 35 ℃.
Example 4
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 1 in that: the preservative is prepared by mixing 0.262kg of 1,2 hexanediol and 0.238kg of p-hydroxyacetophenone, namely the mass ratio of the 1,2 hexanediol to the p-hydroxyacetophenone is 1.1. The specific steps of adding the preservative are as follows: in the fourth step, 1,2 hexanediol and p-hydroxyacetophenone are mixed and added into an emulsifying pot, the mixture is heated to 60 ℃, the stirring is carried out for 5min under the condition that the rotating speed is 60r/min, the preservative is obtained, and then the preservative is added into the material prepared in the third step.
Example 5
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: the preservative is prepared by mixing 0.5kg of 1,2 hexanediol and 0.5kg of p-hydroxyacetophenone, namely the mass ratio of the 1,2 hexanediol to the p-hydroxyacetophenone is 1. The specific steps of adding the preservative are as follows: in the fourth step, 1,2 hexanediol and p-hydroxyacetophenone are mixed and added into an emulsifying pot, the mixture is heated to 85 ℃, the stirring is carried out for 5min under the condition that the rotating speed is 60r/min, the preservative is obtained, and then the preservative is added into the material prepared in the third step.
Example 6
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 0.5kg of pawpaw is also added in the first step, and the amount of water in the first step is 64.46kg.
Example 7
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: in the first step, 2.1kg of pawpaw is also added, and the amount of water in the first step is 62.86kg.
Example 8
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 0.8kg of hawthorn is also added in the step one, and the amount of water used in the step one is 64.16kg.
Example 9
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 3kg of hawthorn is also added in the first step, and the amount of water used in the first step is 61.96kg.
Example 10
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 0.5kg of pawpaw and 0.8kg of hawthorn are also added in the first step, and the dosage of water in the first step is 63.66kg, namely the mass ratio of the pawpaw, the hawthorn and the butcher's broom in the whitening and moisturizing composition is 0.5.
Example 11
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 1kg of pawpaw and 1.6kg of hawthorn are also added in the first step, the amount of water in the first step is 62.36kg, namely the mass ratio of the pawpaw to the hawthorn to the butcher's broom in the whitening and moisturizing composition is 1.6.
Example 12
A method for preparing a whitening and moisturizing composition containing a composite plant extract, which is different from the method in example 11 in that: the dosage of the pawpaw is 1.5kg, the dosage of the hawthorn is 2.4kg, and the dosage of the water is 61.06kg, namely the mass ratio of the pawpaw, the hawthorn and the butcher's broom in the whitening and moisturizing composition is 1.5.
Example 13
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: in the first step, 2kg of chamomile flowers are also added, and the amount of water used in the first step is 62.96kg.
Example 14
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: 3kg of chamomile flowers are also added in the first step, and the amount of water used in the first step is 61.96kg.
Example 15
A method for preparing a whitening and moisturizing composition by compounding plant extracts, which is different from the method in example 3 in that: the auxiliary agent is EDTA disodium.
Example 16
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 5 in that: 1kg of pawpaw, 1.6kg of hawthorn and 2kg of chamomile are also added in the first step, and the amount of water used in the first step is 60.36kg; the auxiliary agent is EDTA disodium.
Comparative example
Comparative example 1
A method for preparing a whitening and moisturizing composition by compounding plant extracts, which is different from the method in example 3 in that: the Ruscus aculeatus and Glycyrrhiza glabra were replaced with equal amounts of water.
Comparative example 2
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: the leaf of bearberry and the licorice root are replaced by the same amount of water.
Comparative example 3
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: the leaf of bearberry and the leaf of Ruscus aculeatus are replaced with the same amount of water.
Comparative example 4
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: equal amount of water was substituted for Glycyrrhiza glabra.
Comparative example 5
A preparation method of a whitening and moisturizing composition containing composite plant extracts is different from that of example 3 in that: the leaf of bearberry, the Ruscus aculeatus and the licorice root are replaced by the same amount of water.
Performance test
1. Tyrosinase activity inhibition assay: firstly, preparing a reagent, specifically as follows:
1) Sodium phosphate buffer (1/15 mol/L, pH = 6.8)
Accurately weighing 1.000g of sodium dihydrogen phosphate and 1.186g of disodium hydrogen phosphate, adding a small amount of deionized water to dissolve, fixing the volume to 500mL, and storing in a refrigerator at 4 ℃ for later use;
2) L-tyrosine solution (7.5 mmol/L)
Accurately weighing 0.272g of L-tyrosine, firstly adding 3-4 drops of concentrated hydrochloric acid, adding about 50mL of deionized water, after the L-tyrosine is completely dissolved by slight heating, adjusting the pH value to about 7 by using a sodium hydroxide solution, and adding deionized water to a constant volume of 200mL;
3) Test solution
Each 0.1g of the compositions of examples 1 to 16 and comparative examples 1 to 5 was accurately weighed, dissolved in 20mL of deionized water to give 5mg/mL of test solutions, and then diluted in duplicate to 2.5mg/mL, 1.25mg/mL, 0.625mg/mL, and 0.3125mg/mL. Table 3 in examples 1 to 13 and comparative examples 1 to 5, each test solution had a concentration of 0.5mg/mL;
4) Positive control (+ CK)
Accurately weighing 0.1g of arbutin powder, dissolving in 20mL of deionized water to obtain 5mg/mL of positive control mother liquor, and diluting to 2.5mg/mL, 1.25mg/mL, 0.625mg/mL and 0.3125mg/mL;
5) The preparation of the tyrosinase liquid uses fresh and intact potatoes to prepare the tyrosinase liquid, and the specific operations are as follows: cleaning potato, pre-cooling at 4 deg.C for about 4 hr, peeling, and cutting into 1.0cm pieces 3 D-form, and freeze at-20 ℃ overnight. Weighing, adding a 4 ℃ precooled sodium phosphate buffer solution according to the mass and volume ratio of 1The obtained tyrosinase crude enzyme solution is stored at 4 ℃ and is used up after 2 hours;
the test is carried out, the total reaction system of each group of tests is 5mL, and the specific design is shown in Table 1:
TABLE 1 5mL test System design
In this system, the final concentration (mg/mL) gradient of the test solution, the positive control mother solution, and arbutin was 0.03125, 0.0625, 0.125, 0.25, and 0.5.
During the experiment, phosphate buffer solution, test solutions of each group, positive control mother solution and enzyme solution are sequentially added into a test tube, and water bath is carried out at 30 ℃ for 10min. The substrate L-tyrosine was then added and the timer was started immediately. The absorbance of each test solution was measured at a wavelength of 475nm at 20min of reaction. During the determination, the inhibition rate of each group of test solution (including negative control) on tyrosinase is calculated by using the following formula with the corresponding negative control as a reference.
Inhibition = [ (A-B)/A ]. Times.100%
Wherein "A" is the absorbance of the standard control and "B" is the absorbance of the test solution (or positive control). Each experiment was done in 3 replicates. The high inhibition rate indicates that the inhibition intensity of the compound on tyrosinase activity is high. Note: when the absorbance is measured by a spectrophotometer, the test solution, the standard control and the positive control are respectively zeroed by the negative control 1, the negative control 2 and the negative control 3.
2. DPPH-free radical scavenging test: the compositions prepared in examples 1 to 16 and comparative examples 1 to 5 were diluted with deionized water to give a test solution having a mass fraction of 1%. Taking 4mL of DPPH-absolute ethanol solution with the concentration of 0.1mmol/L into a container, adding 2mL of test sample, uniformly mixing, keeping out of the sun for 30min at room temperature, measuring the absorbance at the wavelength of 517nm, and recording the absorbance as C1; taking 4mL of absolute ethyl alcohol solution and 2mL of test sample solution, and recording the absorbance value as C2 under the same condition; the absorbance values of 4mL of the anhydrous ethanol solution and 2mL of the anhydrous ethanol solution measured under the same conditions were recorded as C3. DPPH radical clearance was calculated as follows: DPPH radical clearance (%) = [1- (C1-C2)/C3 ] × 100%, averaged over three measurements. DPPH free radical is a stable nitrogen center organic free radical, strong absorption is achieved at 517nm, an ethanol solution of DPPH is purple, when the DPPH free radical scavenger is encountered, single electrons of DPPH are paired to enable the color of the solution to become light, the fading degree of the DPPH free radical is in quantitative relation with the quantity of the received electrons, therefore, a spectrophotometer can be used for carrying out quantitative analysis to detect the condition of scavenging the free radicals, and the higher the scavenging rate is, the stronger the oxidation resistance and the anti-aging capacity of the DPPH free radical is.
3. Testing the water content of the stratum corneum of the skin: 21 test subjects with skin types and facial skin moisture content not obviously different, ages of 25-45 and basic values of 52-55 of upper arm test area capacitance skin moisture measuring instrument are selected.
Before the test, the testers need to clean the inner sides of the upper arms uniformly and clean the upper arms by using dry facial tissues; after cleaning, marking a measuring area on the inner side of the upper arm of the test person; the testers need to sit still for 30min in a room with the ambient temperature of 20-22 ℃ and the humidity of 40-60%, and no water is needed for eating; during the test, 2cm × 2cm test areas are marked on the inner side of the upper arm of the test person, and 2 areas are marked on the same arm at the same time and are spaced by 1cm. One area is only coated with water and is used as a blank control area, and the other area is coated with a sample to be tested; the compositions obtained in examples 1 to 16 and comparative examples 1 to 5 were prepared into 21 test specimens, one specimen for each test person. The measurement of the test zone and the blank control zone was carried out using a Callegari Soft5.5 skin stratum corneum Water content tester, and the average value was calculated for each zone according to 3 replicates.
The blank values of the test and blank control areas were measured and then (2.0. + -. 0.1) mg/cm was measured using a latex finger cuff 2 The amount of test sample applied was uniformly spread over the test area. After application, the skin of 21 subjects was measured in the test area and the blank control area after 30min and 60min, respectivelyThe water content increase was calculated from the water content of the horny layer.
The experimental data for examples 1-16 and comparative examples 1-5 are detailed in Table 2.
TABLE 2
According to the test data of the examples 1-3 and the blank control group in the table 2, the tyrosinase activity inhibition rate and the DPPH-free radical clearance rate of the composition prepared in the examples 1-3 are both more than 80%, which shows that the composition has good whitening and spot-fading effects; the water content of the horny layer is always in a growth state within 60min and has obvious increase compared with a blank control group, which indicates that the moisturizing effect of the composition is better.
The arbutin extract in the composition mainly contains arbutin, and the arbutin can effectively inhibit the activity of biological tyrosinase in the skin and block the formation of melanin; the camelina sativa extract has an effect of eliminating a plurality of free radicals, so that skin pigmentation is reduced, and the combined action of the two helps to improve the whitening and freckle-removing effects of the composition; the Ruscus aculeatus extract can enhance skin cell metabolism and has good antiaging effect; a large amount of phenolic compounds, especially flavone-3-ol, in the strawberry tree fruit extract have biological activities such as oxidation resistance, antibiosis and the like, and the two components act together to further contribute to improving the whitening and anti-aging effects; glycyrrhiza glabra L.extract can penetrate into skin and maintain high activity, has oxygen free radical scavenging ability similar to SOD (superoxide dismutase), and has oxygen free radical resisting ability similar to vitamin E; the chamomile extract in the composition has the effects of relieving, resisting bacteria and resisting oxidation and aging, and the moisture retention and permeation effects are further improved by adding the aloe extract.
The preservative prepared by mixing 1, 2-hexanediol and p-hydroxyacetophenone according to the mass ratio of 1.1 is selected in example 4, the preservative prepared by mixing 1, 2-hexanediol and p-hydroxyacetophenone according to the mass ratio of 1.1 is selected in example 5, and compared with the preservative prepared by using benzyl alcohol as in examples 1-3, the moisture content of the horny layer of the composition prepared in examples 4 and 5 is further improved compared with the moisture content of the composition prepared in examples 1-3, and according to the analysis of the inventor, 1, 2-hexanediol has extremely high hygroscopicity and water retention, and when 1, 2-hexanediol and p-hydroxyacetophenone are mixed and used, the synergistic effect of the 1, 2-hexanediol and the p-hydroxyacetophenone can fully exert the advantages of the 1, 2-hexanediol, so that the comfort feeling and the absorption effect of the skin when the composition is used are improved, and the moisture retention is enhanced.
Examples 6 and 7, in which papaya was added based on example 3, the compositions prepared in examples 6 and 7 had improved tyrosinase activity inhibition and DPPH-radical clearance and significantly improved moisture content in the stratum corneum compared to the composition prepared in example 3, which indicates that the compositions prepared in examples 6 and 7 had improved whitening, anti-aging, moisturizing and skin-lightening properties. According to the analysis of the inventor, the pawpaw is added, so that the composition contains a pawpaw extract, and the effective components of saponin and flavonoid in the pawpaw extract can act together with the effective components of flavone-3-alcohol and the like in the strawberry tree fruit extract, so that the activity of skin cells is greatly improved, the activity of the cells is increased, the effect of the skin cells for absorbing nutrient substances in the composition is better, the effects of fully moisturizing and whitening are achieved, and the whitening and skin brightening effects of the composition are further enhanced.
Examples 8 and 9, in which hawthorn is added to example 3, the tyrosinase activity inhibition rate, the DPPH-radical scavenging rate and the moisture content of the stratum corneum of the skin of the compositions prepared in examples 8 and 9 are significantly improved compared with those of the composition prepared in example 3, which indicates that the overall performance of the compositions prepared in examples 8 and 9 is improved to a certain extent. According to the analysis of the inventor, the hawthorn is added to enable the composition to contain the hawthorn extract, and the hawthorn extract contains various effective components such as hawthorn flavone, vitexin, crataegolic acid, hyperin and epicatechin, so that the hawthorn extract and each plant extract component in the composition can be matched synergistically, and further the composition has the gain effects on various aspects such as whitening, wrinkle resistance, speckle reduction, antibiosis, moisture preservation and the like, and the overall performance of the composition is further improved.
Example 10 is that papaya and hawthorn are added on the basis of example 3, so that the mass ratio of papaya, hawthorn and Ruscus aculeatus is 0.5.
Examples 13 and 14, in addition to example 3, chamomile flowers were added, and the moisture content of the stratum corneum of the skin was improved in the compositions prepared in examples 13 and 14 compared with that of the composition prepared in example 3, which indicates that the moisturizing performance of the compositions prepared in examples 13 and 14 is improved to a certain extent. According to the analysis of the inventor, the composition contains the chamomile extract, the chamomile extract contains flavonoid compounds, amino acids and other components, can act together with the aloe extract, has a good moisturizing effect, regulates the water-oil balance of the skin to a certain extent, effectively replenishes moisture for the skin, and enables the skin to be in a relatively stable state, so that the growth difference of the composition in three time periods of 10min, 30min and 60min is obviously shortened; in addition, the chamomile extract has an anti-inflammatory effect, can reduce the generation of acnes, can resist oxidation and slow down skin aging.
Example 15, in which disodium EDTA was used as an adjuvant, the compositions prepared in example 15 exhibited slightly improved tyrosinase activity inhibition and DPPH-free radical scavenging as well as skin stratum corneum moisture levels as compared to the compositions prepared in example 2, indicating that the overall performance of the compositions prepared in example 15 was improved. The inventor analyzes that the disodium EDTA can play a role of a chelating agent in a composition system, so that the combination and compatibility of plant extracts, solvents and other components in the composition are improved, the components in the composition are uniformly matched, and the effect of the composition is improved better.
Example 16 the compositions prepared in example 16 have better improved properties than the compositions prepared in example 3 by combining and formulating the components and processes with beneficial effects in the above examples, and the compositions prepared in example 16 have better whitening and moisturizing effects through the combination of various substances in the composition system and the selection of more appropriate additives.
Comparative example 5 the bearberry leaf, the ruscus aculeatus and the glycyrrhiza glabra were all replaced with the same amount of water, the composition prepared in comparative example 5 did not have the bearberry leaf extract, the ruscus aculeatus extract and the glycyrrhiza glabra extract, and the composition prepared in comparative example 5 was inferior in each performance. In the comparative example 1, the Ruscus aculeatus and the Glycyrrhiza glabra are replaced by the same amount of water, the bearberry leaf extract is added in the composition of the comparative example 1 compared with the composition of the comparative example 5, the tyrosinase activity inhibition rate and the free radical clearance rate of the composition prepared in the comparative example 1 are improved compared with those of the composition of the comparative example 5, and the bearberry leaf extract is helpful for improving the whitening and anti-aging effects; the Ruscus aculeatus extract is more in the comparative example 2 than in the comparative example 5, and the tyrosinase activity inhibition rate and the free radical clearance rate of the composition prepared in the comparative example 2 are improved compared with those of the composition prepared in the comparative example 5, so that the Ruscus aculeatus extract is also beneficial to improving the whitening and anti-aging effects; comparative example 3 has more glycyrrhiza glabra extract than the composition of comparative example 5, and the composition prepared in comparative example 3 has improved tyrosinase activity inhibition rate and free radical scavenging rate compared with the composition of comparative example 5, which indicates that glycyrrhiza glabra extract is helpful for improving whitening anti-aging effect, but the improvement effect is not as good as that of bearberry leaf extract and Ruscus aculeatus extract.
The composition prepared in the comparative example 4 has a significantly weaker effect of improving the tyrosinase activity inhibition rate than the composition prepared in the comparative example 5 than the composition prepared in the comparative example 1 and the composition prepared in the comparative example 2, which shows that the combined use of the bearberry extract and the butcher's broom extract can generate a certain inhibition effect on the whitening effect of the composition; example 3 in comparison with comparative example 4, the glycyrrhiza glabra extract is added, and the tyrosinase activity inhibition rate and the increase amount of the skin moisture content of the composition prepared in example 3 are obviously improved in comparison with those of the composition prepared in comparative example 5, which shows that the addition of the glycyrrhiza glabra extract helps to relieve the inhibition effect between the bearberry leaf extract and the ruscus aculeatus extract, and further improves the moisturizing effect of the composition. The inventor analyzes that certain synergistic effect can be generated among the bearberry leaf extract, the Ruscus aculeatus extract and the Glycyrrhiza glabra extract, so that effective components playing a role in whitening in the bearberry leaf extract, the Ruscus aculeatus extract and the Glycyrrhiza glabra extract are converted, the inhibition problem is solved, and the moisturizing effect is further improved.
The above are preferred embodiments of the present application, and the scope of protection of the present application is not limited thereto, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (8)
1. A preparation method of a whitening and moisturizing composition containing compound plant extracts is characterized by comprising the following steps: the method comprises the following steps:
step one, uniformly mixing bearberry leaves, butcher's broom, strawberry fruits, shepherd's purse, glabrous licorice, aloe, butanediol and water, and carrying out dissolving and soaking for 30-35min to obtain a raw material mixture;
step two, heating the raw material mixture to more than 100 ℃, performing reflux circulation, stopping heating after heating for 1.2-1.5h, and keeping the temperature for 30-35min;
step three, cooling to 30-35 ℃, and filtering to obtain an intermediate mixture;
adding a preservative and an auxiliary agent into the intermediate mixture, uniformly stirring at a constant temperature of 30-35 ℃, filtering, standing for 12 hours to allow liquid to be layered, discharging supernatant liquid, and reserving lower layer liquid to obtain the whitening and moisturizing composition containing the composite plant extract;
the mass percentages of the components are as follows: 2-4wt% of bearberry leaves, 1-3wt% of Ruscus aculeatus, 1-3wt% of strawberry tree fruits, 1-3wt% of shepherd's purse, 2-4wt% of Glycyrrhiza glabra, 1-3wt% of aloe, 19-21wt% of butanediol, 0.5-1wt% of preservative, 0.02-0.04wt% of auxiliary agent and water which are added to 100wt%.
2. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1, wherein the method comprises the steps of: in the fourth step, the preparation method of the preservative comprises the following steps: 1,2 hexanediol and p-hydroxyacetophenone are mixed according to the mass ratio of (1-1.1): 1, mixing and heating to 60-85 ℃, and uniformly stirring to obtain the preservative.
3. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1 or 2, wherein: 0.5wt% -2.1wt% of pawpaw is also added in the first step.
4. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1 or 2, wherein: 0.8 to 3 weight percent of hawthorn is also added in the first step.
5. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1 or 2, wherein: 1 to 1.5 weight percent of pawpaw and 1.6 to 2.4 weight percent of hawthorn are also added in the first step.
6. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1 or 2, wherein: in the first step, 2-3wt% of chamomile flowers are also added.
7. The method for preparing a whitening and moisturizing composition containing composite plant extracts as claimed in claim 1, wherein the method comprises the steps of: the auxiliary agent is EDTA disodium.
8. A whitening and moisturizing composition containing compound plant extracts is characterized in that: the whitening and moisturizing composition containing the composite plant extract as set forth in any one of claims 1 to 7.
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| CN112494406A (en) * | 2020-12-25 | 2021-03-16 | 云南科妍医药生物科技有限公司 | Whitening and freckle-removing cosmetic and preparation method thereof |
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