CN114304169A - A kind of bactericidal composition containing selemenine analog and small molecular phenolic compound and its application in preventing and controlling plant diseases - Google Patents
A kind of bactericidal composition containing selemenine analog and small molecular phenolic compound and its application in preventing and controlling plant diseases Download PDFInfo
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- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 claims description 3
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- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 claims 1
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Abstract
本发明公开了一种含有白叶藤碱或新白叶藤碱和小分子酚类化合物的杀菌组合物，有效成分为白叶藤碱，新白叶藤碱和百里酚，香芹酚，6‑叔丁基间甲酚，2‑异丙基苯酚，2‑叔丁基苯酚，2,4‑二叔丁基苯酚，白叶藤碱或新白叶藤碱和小分子酚类化合物的质量份数比为1:1，1:10，1:20，1:50，1:100；本发明杀菌组合物可用于防治立枯丝核菌、油菜菌核病、番茄灰霉病、小麦赤霉病、稻瘟病和棉花枯萎病中的应用。The invention discloses a bactericidal composition containing selemenine or selemenine and small-molecule phenolic compounds. 6-tert-butyl-m-cresol, 2-isopropylphenol, 2-tert-butylphenol, 2,4-di-tert-butylphenol, selenium or neoselenium and small molecule phenolic compounds The mass-to-number ratios are 1:1, 1:10, 1:20, 1:50, and 1:100; the bactericidal composition of the present invention can be used for the prevention and treatment of Rhizoctonia solani, Sclerotinia sclerotiorum, tomato gray mold, wheat Applications in head blight, rice blast and cotton wilt.
Description
技术领域technical field
本发明涉及的杀菌组合物，是以白叶藤碱或新白叶藤碱和小分子酚类化合物为有效成分，适用于防治立枯丝核菌、油菜菌核病、番茄灰霉病、小麦赤霉病、稻瘟病和棉花枯萎病引起的植物病害上的应用。The bactericidal composition involved in the present invention is based on selenium or neoselenium and small molecular phenolic compounds as active ingredients, and is suitable for preventing and treating Rhizoctonia solani, Sclerotinia sclerotiorum, tomato botrytis, and wheat. Application to plant diseases caused by head blight, rice blast and cotton wilt.
背景技术Background technique
在防治植物病原真菌的诸多措施中，化学防治仍是目前主流的防治手段，在防治初期一般都能取得高效的防治效果。但是由于长期的不合理使用，细菌和真菌对这些化学杀菌剂产生了不同程度的抗性，导致加大用药量才能达到理想的杀菌效果，进而使药物残留增多，毒副作用增强。为了应对这种耐药性，减少化学杀菌剂的用量，联合用药或复配显得尤为重要，其不仅可以减少药物的用量，增加病菌对杀菌剂的敏感性，扩大抗菌谱，减少环境的污染，更重要的是可以减缓杀菌剂耐药性的出现。Among the many measures to control phytopathogenic fungi, chemical control is still the mainstream control method at present, and generally effective control effects can be achieved in the early stage of control. However, due to long-term irrational use, bacteria and fungi have developed different degrees of resistance to these chemical fungicides, resulting in increased drug dosage to achieve the desired bactericidal effect, which in turn increases drug residues and increases toxic and side effects. In order to cope with this drug resistance and reduce the dosage of chemical fungicides, it is particularly important to combine medication or compounding, which can not only reduce the dosage of drugs, increase the sensitivity of bacteria to fungicides, expand the antibacterial spectrum, and reduce environmental pollution. More importantly, the emergence of fungicide resistance can be slowed down.
白叶藤碱和新白叶藤碱是西非国家药用植物白叶藤(C.sanguinolenta)中的生物碱。其具有多种生物活性，包括细胞毒性，抗细菌，抗真菌和抗寄生虫等活性，因其结构新颖，活性多样，成为目前天然药物研究的热点之一。Selmenine and Selmenine are alkaloids in C. sanguinolenta, a medicinal plant in West Africa. It has a variety of biological activities, including cytotoxicity, antibacterial, antifungal and antiparasitic activities. Because of its novel structure and diverse activities, it has become one of the hot spots in natural medicine research.
酚类化合物是一类比较常见的生物活性小分子化合物，在自然界中酚类化合物是植物体内重要的次生代谢产物，据报道酚类化合物中的简单酚，黄酮及醌类等在植物体外对植物病原真菌菌丝生长与孢子萌发具有抑制作用，在植物体内对植物生长发育有一定的调节作用，同时在植物抗病，基因的诱导表达，信号传导等方面也有重要作用。此外，研究发现丁香酚(Eugenol)对烟草疫霉(Phytophthora)的MIC＝200μg/mL；紫檀芪(Pterostilbene)在50mg/L时能够完全抑制灰霉菌B.cinerea的孢子萌发；香芹酚(carvacrol)在25μg/mL时，对匍枝根霉(Rhizopus Stolonifer)的孢子萌发抑制率达到了100％。值得关注的是尽管它们具有很高的抗菌和杀虫功效，但他们对恒温动物的毒性却很低，因此酚类化合物在抗菌方面具有较好的应用前景。Phenolic compounds are a relatively common class of biologically active small molecule compounds. In nature, phenolic compounds are important secondary metabolites in plants. Plant pathogenic fungi have an inhibitory effect on mycelial growth and spore germination, and have a certain regulatory effect on plant growth and development in plants. In addition, the study found that the MIC of Eugenol against Phytophthora = 200 μg/mL; Pterostilbene at 50 mg/L can completely inhibit the spore germination of Botrytis cinerea; carvacrol (carvacrol) ) at 25 μg/mL, the spore germination inhibition rate of Rhizopus Stolonifer reached 100%. It is worth noting that although they have high antibacterial and insecticidal efficacy, their toxicity to warm-blooded animals is very low, so phenolic compounds have good application prospects in antibacterial.
通过大量试验发现，将白叶藤碱或新白叶藤碱和小分子酚类化合物组合应用，在防治立枯丝核菌、油菜菌核病、番茄灰霉病、小麦赤霉病、稻瘟病和棉花枯萎病具有明显的协同增效作用。其防治效果提高的同时，还可减少用药量，符合“农药零增长”的需求。且目前，尚无关于白叶藤碱或新白叶藤碱和小分子酚类化合物复配的相关报道。Through a large number of experiments, it has been found that the combination application of selenium or neoselenium and small molecular phenolic compounds is effective in the control of Rhizoctonia solani, Sclerotinia sclerotiorum, tomato gray mold, wheat scab, rice blast It has obvious synergistic effect with cotton fusarium wilt. While improving the control effect, it can also reduce the dosage of pesticides, which is in line with the demand of "zero growth of pesticides". And at present, there is no relevant report on the compounding of selemenine or new selemenine and small molecular phenolic compounds.
发明内容SUMMARY OF THE INVENTION
本发明的目的在于提供一种含白叶藤碱或新白叶藤碱和小分子酚类化合物的杀菌剂组合物，用于防治立枯丝核菌、油菜菌核病、番茄灰霉病、小麦赤霉病、稻瘟病和棉花枯萎病应用。The purpose of the present invention is to provide a kind of fungicide composition containing selenium or neoselenium and small molecular phenolic compounds, for preventing and treating Rhizoctonia solani, Sclerotinia sclerotiorum, tomato botrytis, Wheat scab, rice blast and cotton wilt applications.
为实现上述目的，本发明的技术方案如下：For achieving the above object, technical scheme of the present invention is as follows:
一种含白叶藤碱或新白叶藤碱和小分子酚类化合物的杀菌剂组合物，包括活性组分A与活性组分B，所述活性组分A为白叶藤碱或新白叶藤碱，所述活性组分B为小分子酚类化合物，活性组分A与活性组分B的重量份数比为1:1，1:10，1:20，1:50，1:100。A fungicide composition containing selemenine or selenium and small molecular phenolic compounds, comprising active component A and active component B, and the active component A is selenium or selenium Yemenine, the active component B is a small-molecule phenolic compound, and the weight ratio of the active component A to the active component B is 1:1, 1:10, 1:20, 1:50, 1:1 100.
本发明提供的杀菌剂组合物有如下有益效果：The bactericide composition provided by the invention has the following beneficial effects:
1)本发明提供的杀菌组合物，在一定配比范围内表现出明显的增效作用，组合物的防治效果比单剂有了明显提高。1) The bactericidal composition provided by the present invention exhibits an obvious synergistic effect within a certain proportioning range, and the control effect of the composition is obviously improved compared with that of a single agent.
2)本发明提供的杀菌组合物的有效成分作用机制各不相同，组合物的可望能延缓或克服病害抗药性的产生，延长药剂的使用寿命，有利于病害的综合治理。2) The action mechanisms of the active ingredients of the bactericidal composition provided by the present invention are different, and the composition is expected to delay or overcome the generation of disease resistance, prolong the service life of the medicine, and be beneficial to the comprehensive treatment of diseases.
具体实施方式Detailed ways
为了更好的理解本发明的实质，通过以下实施例进一步说明，但本发明不限于这些实施例。在这些实施例中，除另有说明外，所有百分比均为重量百分比。In order to better understand the essence of the present invention, the following examples are used to further illustrate, but the present invention is not limited to these examples. In these examples, all percentages are by weight unless otherwise indicated.
实施例1：一种含白叶藤碱或新白叶藤碱和小分子酚类化合物的杀菌剂组合物，包括活性组分A与活性组分B(如结构式1所示)，所述活性组分A为白叶藤碱或新白叶藤碱，所述活性组分B为小分子酚类化合物。Embodiment 1: a fungicide composition containing selemenine or selemenine and a small molecule phenolic compound, comprising active component A and active component B (as shown in structural formula 1), the active Component A is phyllostachys or selemenine, and the active component B is a small-molecule phenolic compound.
实施例2：白叶藤碱和活性组分B混配对油菜菌核病的室内活性测定Example 2: Indoor activity determination of sclerotinia sclerotiorum by mixing selemenine and active component B
本实验中所用的植物病原菌为实验室4℃保存的菌种，采用的培养基为马铃薯琼脂葡萄糖培养基(简称PDA)。PDA培养基配方：马铃薯(去皮)200g，葡萄糖20g，琼脂15g，蒸馏水1000mL，自然PH。The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato agar-dextrose medium (PDA for short). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.
杀菌剂的混配增效作用依照Wadley(1967)公式计算不同药剂混合后的协同作用系数：The synergistic effect of the mixing of fungicides is calculated according to the formula of Wadley (1967) to calculate the synergy coefficient after mixing of different agents:
式中a,b代表白叶藤碱或新白叶藤碱(A)，小分子酚类化合物(B)两种药剂在混合物中所占比率EC50(A)，EC50(B)分别表示实际观测EC50值，EC50(Exp)表示A，B两种药剂按a:b混合后的理论EC50值，EC50(Obs)为A，B两种药剂按a:b混合后的实际观测EC50值。SR>1.5为增效；SR<0.5为拮抗；SR介于0.5和1.5之间表示相加作用。In the formula, a and b represent selenium or selenium (A), and the ratios of the two agents in the mixture of small molecular phenolic compounds (B) EC 50 (A), EC 50 (B) respectively represent The actual observed EC 50 value, EC 50 (Exp) represents the theoretical EC 50 value of the two agents A and B mixed according to a:b, and EC 50 (Obs) is the actual value of the two agents A and B mixed according to a:b Observe EC50 values. SR>1.5 is synergistic; SR<0.5 is antagonistic; SR between 0.5 and 1.5 indicates additive effect.
白叶藤碱和活性组分B混配对油菜菌核病的室内毒力增效作用如表1所示。Table 1 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on Sclerotinia sclerotiorum.
表1.白叶藤碱和活性组分B混配对油菜菌核病的室内毒力增效作用Table 1. Indoor virulence synergistic effects of sclerophylline and active component B mixture on Sclerotinia sclerotiorum
由表1可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对油菜菌核病具有明显的增效作用，尤其是白叶藤碱和P4在质量比1:50下，SR高达39.75。It can be seen from Table 1 that the mixing mass ratio of selemenine and active component B at 1:1, 1:10, 1:20, 1:50, 1:100 has obvious synergistic effect on rape sclerotinia , especially at the mass ratio of selemenine and P4, the SR was as high as 39.75.
实施例3：白叶藤碱和活性组分B混配对立枯丝核菌的室内活性测定Example 3: Indoor activity assay of selemenine and active component B mixed against Rhizoctonia solani
白叶藤碱和活性组分B混配对立枯丝核菌的室内毒力增效作用如表2所示。Table 2 shows the indoor virulence synergistic effect of selemenine and active component B mixture on Rhizoctonia solani.
表2.白叶藤碱和活性组分B混配对立枯丝核菌的室内毒力增效作用Table 2. Indoor virulence synergistic effect of selemenine and active component B mixture on Rhizoctonia solani
由表2可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对立枯丝核菌具有明显的增效作用，尤其是白叶藤碱和P1在质量比1:1下，SR高达9.75。It can be seen from Table 2 that the mixed mass ratio of selemenine and active component B has obvious synergistic effect on Rhizoctonia solani , especially at the mass ratio of selemenine and P1, the SR was as high as 9.75.
实施例4：白叶藤碱和活性组分B混配对小麦赤霉病的室内活性测定Example 4: Indoor activity assay of selemenine and active component B mixture on wheat scab
白叶藤碱和活性组分B混配对小麦赤霉病的室内毒力增效作用如表3所示。Table 3 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on wheat scab.
表3.白叶藤碱和活性组分B混配对小麦赤霉病的室内毒力增效作用Table 3. Indoor virulence synergistic effect of selemenine and active component B mixture on wheat scab
由表3可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对小麦赤霉病的增效作用不是很明显，只有白叶藤碱和P2在质量比为1:50时，SR＝1.53；白叶藤碱和P5在质量比为1:100时，SR＝1.52。其余组合物更多的表现为相加作用，其次是拮抗作用。It can be seen from Table 3 that the synergistic effect on wheat scab is not very good when the mass ratio of selemenine and active component B is 1:1, 1:10, 1:20, 1:50 and 1:100. Obviously, only when the mass ratio of selemenine and P2 is 1:50, SR=1.53; when the mass ratio of selemenine and P5 is 1:100, SR=1.52. The remaining compositions exhibited more additive effects, followed by antagonistic effects.
实施例5：白叶藤碱和活性组分B混配对番茄灰霉病的室内活性测定Example 5: Determination of indoor activity of selemenine and active component B mixture on tomato botrytis cinerea
白叶藤碱和活性组分B混配对番茄灰霉病的室内毒力增效作用如表4所示。Table 4 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on tomato botrytis cinerea.
表4.白叶藤碱和活性组分B混配对番茄灰霉病的室内毒力增效作用Table 4. Indoor virulence synergistic effect of selemenine and active component B on tomato botrytis cinerea
由表4可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对番茄灰霉病具有明显的增效作用，尤其是白叶藤碱和P6在质量比1:10时，SR高达14.44。It can be seen from Table 4 that when the mass ratio of selemenine and active component B is 1:1, 1:10, 1:20, 1:50 and 1:100, it has obvious synergistic effect on tomato botrytis cinerea , especially when the mass ratio of selemenine and P6 was 1:10, the SR was as high as 14.44.
实施例6：白叶藤碱和活性组分B混配对稻瘟病的室内活性测定Example 6: Indoor activity determination of the mixture of selemenine and active component B on rice blast
白叶藤碱和活性组分B混配对稻瘟病的室内毒力增效作用如表5所示。Table 5 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on rice blast.
表5.白叶藤碱和活性组分B混配对稻瘟病的室内毒力增效作用Table 5. Indoor virulence synergistic effect of selemenine and active component B mixture on rice blast
由表5可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对番茄灰霉病具有明显的增效作用，尤其是白叶藤碱和P1在质量比1:1时，SR高达2.52。It can be seen from Table 5 that when the mass ratio of selemenine and active component B is 1:1, 1:10, 1:20, 1:50 and 1:100, it has obvious synergistic effect on tomato botrytis cinerea , especially when the mass ratio of selemenine and P1 was 1:1, the SR was as high as 2.52.
实施例7：白叶藤碱和活性组分B混配对棉花枯萎病的室内活性测定Example 7: Indoor activity determination of selemenine and active component B mixture on cotton fusarium wilt
白叶藤碱和活性组分B混配对棉花枯萎病的室内毒力增效作用如表6所示。Table 6 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on cotton wilt.
表6.白叶藤碱和活性组分B混配对棉花枯萎病的室内毒力增效作用Table 6. Indoor virulence synergism of selemenine and active component B mixture on cotton wilt
由表6可知，白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20，1:50，1:100时对棉花枯萎病的增效作用不是很明显，只有白叶藤碱和P2在质量比为1:1，1:10时，SR＝2.01，1.86。其余组合物更多的表现为相加作用，其次是拮抗作用。It can be seen from Table 6 that the synergistic effect on cotton fusarium wilt is not obvious when the mass ratio of selemenine and active component B is 1:1, 1:10, 1:20, 1:50, and 1:100. , only when the mass ratio of selemenine and P2 is 1:1, 1:10, SR=2.01, 1.86. The remaining compositions exhibited more additive effects, followed by antagonistic effects.
实施例8：新白叶藤碱和活性组分B混配对油菜菌核病的室内活性测定Example 8: Indoor activity determination of Sclerotinia sclerotiorum mixed with active component B on Sclerotinia sclerotiorum
新白叶藤碱和活性组分B混配对油菜菌核病的室内毒力增效作用如表7所示。Table 7 shows the indoor virulence synergistic effect of the mixture of sclerophylline and active component B on Sclerotinia sclerotiorum.
表7.新白叶藤碱和活性组分B混配对油菜菌核病的室内毒力增效作用Table 7. Indoor virulence synergistic effect of sclerotin and active component B mixture on Sclerotinia sclerotiorum
由表7可知，新白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20时对油菜菌核病的增效作用明显，尤其是新白叶藤碱和P4在质量比1:1时，SR高达3.93。It can be seen from Table 7 that the synergistic effect of sclerotinia sclerotiorum on rapeseed sclerotinia is obvious when the mass ratio of selenium and active component B is 1:1, 1:10, and 1:20, especially sclerophylline. When the mass ratio is 1:1 with P4, the SR is as high as 3.93.
实施例9：新白叶藤碱和活性组分B混配对立枯丝核菌的室内活性测定Example 9: Determination of indoor activity of rhizoctonia solani mixed with selemenine and active component B
新白叶藤碱和活性组分B混配对立枯丝核菌的室内毒力增效作用如表8所示。Table 8 shows the indoor virulence synergistic effect of the mixture of neophyllaline and active component B on Rhizoctonia solani.
表8.新白叶藤碱和活性组分B混配对立枯丝核菌的室内毒力增效作用Table 8. Indoor virulence synergism of the mixture of serophylline and active component B on Rhizoctonia solani
由表8可知，新白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20时对立枯丝核菌的增效作用不是很明显，只有新白叶藤碱和P3在质量比为1:20时，SR＝1.65。其余组合物更多的表现为相加作用，其次是拮抗作用。It can be seen from Table 8 that the synergistic effect on Rhizoctonia solani is not very obvious when the mass ratio of selenium and active component B is 1:1, 1:10, and 1:20. When the mass ratio of alkali and P3 is 1:20, SR=1.65. The remaining compositions exhibited more additive effects, followed by antagonistic effects.
实施例10：新白叶藤碱和活性组分B混配对小麦赤霉病的室内活性测定Example 10: Determination of indoor activity of the mixture of selemenine and active component B on wheat scab
新白叶藤碱和活性组分B混配对小麦赤霉病的室内毒力增效作用如表9所示。Table 9 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on wheat scab.
表9.新白叶藤碱和活性组分B混配对小麦赤霉病的室内毒力增效作用Table 9. Indoor virulence synergism of the mixture of selemenine and active component B on wheat scab
由表9可以看出，新白叶藤碱和活性组分B在1:1，1:10，1:20质量比时对小麦赤霉病的防治并没有协同作用，大部分为相加作用，同时也存在几组拮抗作用。It can be seen from Table 9 that when the mass ratios of selemenine and active component B are 1:1, 1:10, and 1:20, there is no synergistic effect on the control of wheat scab, and most of them are additive effects. , and there are also several groups of antagonistic effects.
实施例11：新白叶藤碱和活性组分B混配对番茄灰霉病的室内活性测定Example 11: Determination of indoor activity of the mixture of selemenine and active component B on tomato botrytis cinerea
新白叶藤碱和活性组分B混配对番茄灰霉病的室内毒力增效作用如表10所示。Table 10 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on tomato botrytis cinerea.
表10.新白叶藤碱和活性组分B混配对番茄灰霉病的室内毒力增效作用Table 10. Indoor virulence synergistic effect of the mixture of selemenine and active component B on tomato botrytis cinerea
由表10可知，新白叶藤碱和活性组分B混配质量比在1:1，1:10，1:20时对番茄灰霉病的增效作用明显，尤其是新白叶藤碱和P1在质量比1:1时，SR高达5.61。It can be seen from Table 10 that the synergistic effect on tomato botrytis cinerea is obvious when the mass ratio of selemenine and active component B is 1:1, 1:10 and 1:20, especially selemenine. When the mass ratio is 1:1 with P1, the SR is as high as 5.61.
实施例12：新白叶藤碱和活性组分B混配对稻瘟病的室内活性测定Example 12: Determination of indoor activity of the mixture of neophyllaine and active component B on rice blast
新白叶藤碱和活性组分B混配对稻瘟病的室内毒力增效作用如表11所示。Table 11 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on rice blast.
表11.新白叶藤碱和活性组分B混配对稻瘟病的室内毒力增效作用Table 11. Indoor virulence synergistic effect of the mixture of selemenine and active component B on rice blast
由表11可以看出，新白叶藤碱和活性组分B在1:1，1:10，1:20质量比时对稻瘟病的防治并没有协同作用，大部分为拮抗作用，同时也存在几组相加作用。It can be seen from Table 11 that when the mass ratio of selemenine and active component B is 1:1, 1:10, and 1:20, there is no synergistic effect on the control of rice blast, and most of them are antagonistic. There are several sets of additive effects.
实施例13：新白叶藤碱和活性组分B混配对棉花枯萎病的室内活性测定Example 13: Determination of indoor activity of the mixture of selemenine and active component B on cotton fusarium wilt
新白叶藤碱和活性组分B混配对棉花枯萎病的室内毒力增效作用如表12所示。Table 12 shows the indoor virulence synergistic effect of the mixture of selemenine and active component B on cotton wilt.
表12.新白叶藤碱和活性组分B混配对棉花枯萎病的室内毒力增效作用Table 12. Indoor virulence synergism of the mixture of Neoselemenine and active component B on cotton wilt
由表12可以看出，新白叶藤碱和活性组分B在1:1，1:10，1:20质量比时对棉花枯萎病的防治并没有协同作用，大部分为相加作用，同时也存在几组拮抗作用。As can be seen from Table 12, selemenine and active component B in the mass ratio of 1:1, 1:10, 1:20 have no synergistic effect on the control of cotton fusarium wilt, and most of them are additive effects, There are also several groups of antagonistic effects.
综上所述，可以看出白叶藤碱和小分子酚类化合物组合的抗菌活性较新白叶藤碱组合物要好，尤其是白叶藤碱：P4＝1:50，抗油菜菌核病时，SR高达39.75。这比产生协同增效的条件(SR>1.5)高很多。在防治油菜菌核病时，出现了很多协同增效的组合，例如在白叶藤碱：P1，白叶藤碱：P2和白叶藤碱：P3均为1:1时，其SR分别为12.42，11.74和13.09。To sum up, it can be seen that the antibacterial activity of the combination of phyllostachys and small molecular phenolic compounds is better than that of the new phyllostachys, especially the phyllostachys: P4=1:50, resistant to Sclerotinia sclerotiorum , the SR is as high as 39.75. This is much higher than the condition that produces synergy (SR>1.5). In the control of rape sclerotinia, there are many synergistic combinations. For example, when the selenium: P1, the selenium: P2 and the sclerotin: P3 are all 1:1, the SRs are respectively 12.42, 11.74 and 13.09.
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