CN114304162B - Bactericidal composition and preparation and application thereof - Google Patents
Bactericidal composition and preparation and application thereof Download PDFInfo
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Abstract
The invention relates to the field of agricultural prevention and treatment, and particularly relates to a bactericidal composition, a preparation and an application thereof, wherein active ingredients of the bactericidal composition comprise an active ingredient A and an active ingredient B, the active ingredient A is a compound shown in a formula I, and the active ingredient B is any one of bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin; the structural formula of the bactericidal compound is shown as a formula I. The bactericidal composition or the preparation thereof can increase the bactericidal effect, reduce the dosage, delay the generation of drug resistance, have obvious effect of controlling plant diseases and can improve the yield and the quality of crops.
Description
Technical Field
The invention relates to the field of pesticides, in particular to a bactericidal composition, and a preparation and application thereof.
Background
The existing plant diseases are one of the important reasons influencing the health of crops, and the drug resistance of pathogenic fungi/bacteria is enhanced year by year due to long-term use of the same medicament, so that the control difficulty is increased continuously. In recent years, with the increasing requirements and importance of people on environmental ecology, food safety and the like, some traditional sterilization varieties are forbidden and limited in many countries and regions due to the limitation and influence of toxicity, residues, environmental factors, resistance and the like. The problems of pesticide residue, environmental toxicity, pest resistance and the like caused by long-term unscientific use of the bactericide developed and marketed in the eighties and ninety years of the twentieth century are increasingly serious, and some medicaments even generate cross resistance. Continuous, large-amount and single-use of the bactericide is a main reason causing rapid generation of drug resistance of germs.
Compared with developed bactericides, newly developed bactericidal compounds have unique bactericidal mechanisms due to brand new chemical structures, often have higher activity and better control effect, are not easy to generate drug resistance, have no cross resistance with other bactericides, and can play a role in synergism, so the bactericide has wide application prospect.
Compounding pesticides and preparing the pesticides into a compound preparation are effective and quick ways for solving the problem. The bactericide with a new action mechanism is substituted or used alternatively. In order to overcome and make up for the above deficiencies, research departments at home and abroad often mix a pesticide with other pesticides to improve the pesticide effect, avoid the generation of resistance, prolong the control duration, expand the bactericidal spectrum and reduce the cost.
Disclosure of Invention
Based on the situation, the invention aims to provide a pesticide sterilization composition containing a brand-new compound and a preparation thereof, which are mainly used for preventing and treating plant pathogenic fungi and bacteria.
In order to achieve the above object, the present invention provides a fungicidal compound, wherein the fungicidal compound has a structural formula shown in formula I:
the chemical formula of the compound shown in the formula I is as follows: c 12 H 12 N 5 F。
The active ingredients of the bactericidal composition comprise an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound shown in a formula I, and the active ingredient B is any one of bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin.
Further, the mass ratio of the active ingredient A to the active ingredient B is 50;
further, the mass ratio of the active ingredient A to the bronopol is 20;
further, the mass ratio of the active ingredient A to the amino-oligosaccharin is 10;
further, the mass ratio of the active ingredient A to the zhongshengmycin is 20 to 1, preferably 16;
further, the mass ratio of the active ingredient A to the pyraclostrobin is 20-1, preferably 15;
further, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1-95 wt%, preferably 3-60 wt%, based on the total weight of the bactericidal composition being 100 wt%;
preferably, the bactericidal composition further comprises an auxiliary material, wherein the auxiliary material is at least one of an emulsifier, a dispersant, a wetting agent, a spreader, a stabilizer, a defoaming agent, a synergist, a penetrating agent, a sticking agent, a safener, a carrier, a thickener, a disintegrant, an antifreezing agent, a solvent, a preservative and a filler.
The application of the bactericidal composition in preventing and treating crop pathogenic fungi and/or bacteria.
The wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignosulfonate, sodium dodecyl sulfate, dioctyl sodium sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether, silkworm excrement, chinese honeylocust powder, soapberry powder, SOPA, detergent, emulsifier 2000 series polycarboxylate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkyl naphthalene sulfonate, alkyl benzene sulfonate, polyoxyethylene ether phosphate, styrene-based phenol polyoxyethylene ether phosphate, polyoxyethylene polyoxypropylene block copolymer, vegetable oil epoxy ethane addition compound and derivative, styrene maleic anhydride copolymer, polyvinyl alcohol and acrylic acid copolymer;
the dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol, ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or
The emulsifier is selected from one or more of alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, castor oil polyoxyethylene ether, alkylphenol ether phosphate, oleic acid ester emulsifier and alkylbenzene sulfonate;
the thickener is one or more selected from xanthan gum, starch, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black;
disintegrating agent the disintegrating agent is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid;
the antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorohydrocarbons and inorganic salts;
the defoaming agent is selected from one or more of C10-C20 saturated fatty acid compounds, silicone oil, silicone compounds and C8-C10 fatty alcohol;
the solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil derivative and deionized water;
the preservative is selected from one or more of propionic acid, propionic acid sodium salt, sorbic acid sodium salt, sorbic acid potassium salt, benzoic acid sodium salt, p-hydroxybenzoic acid methyl ester, carbazone and 1, 2-benzisothiazoline 3-one;
the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite and starch;
the synergist is selected from synergistic phosphorus and synergistic ether;
the carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil and vegetable oil derivative.
The preparation prepared from the bactericidal composition has a dosage form selected from any one of wettable powder, granules, soluble powder, soluble granules, soluble tablets, water dispersible granules, wettable powder, microcapsule granules, powder, water dispersible tablets, microcapsule suspending agents, dispersible agents, emulsifiable agents, aqueous emulsion, microemulsion, suspending agents, dispersible oil suspending agents, soluble agents and ultra-low volume liquid agents.
The method of forming the above dosage forms is not particularly limited, and those skilled in the art can form various dosage forms provided by the present invention according to the common general knowledge. The present invention will be described in detail below by way of examples. In the following examples, unless otherwise specified, all the raw materials used are commercially available products.
The preparation is applied to control crop pathogenic fungi and/or bacteria.
Has the advantages that: by compounding the compound shown in the formula I with any one of bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin, the composition obtains an excellent disease control effect, can effectively improve the control effect on target organisms, reduce the use amount of pesticides, reduce agricultural cost, is friendly to crops, non-target organisms and environment, increases the quick-acting property of the pesticide on pathogenic bacteria, delays the generation of drug resistance of the pathogenic bacteria, and prolongs the drug duration.
The bactericidal compound, the bactericidal composition or the preparation thereof can increase the bactericidal effect, reduce the dosage, delay the generation of drug resistance, have obvious effect on preventing and treating plant diseases and can improve the yield and the quality of crops.
Detailed Description
To make the technical solutions, objects, and advantages of the present invention more apparent, the present invention is described with the following specific examples, but the present invention may be implemented in various forms and should not be limited by the embodiments set forth herein.
The synthesis method of the compound shown in the formula I is as follows:
under the catalysis of ascorbic acid, mixing compound 1-1, compound 1-2 and a catalyst in a molar ratio of 1: dissolving 0.1 in acetone, and stirring at constant temperature of 70 ℃ for 24h to obtain the compound shown in the formula I.
Preparation example 1:10% wettable powder of the compound of formula I-bronopol (1
5g of compound raw drug of formula I, 5g of bronopol raw drug, 5g of calcium dodecyl sulfate, 2g of sodium lignosulfonate, 1g of ammonium sulfate and 100g of bentonite are added, pre-crushed and mixed uniformly, and then crushed by an airflow crusher until the fineness of at least 98wt% meets the requirement that the mixture passes through a 45 mu m test sieve, so as to prepare the wettable powder with the mass content of the active ingredients of 10 wt%.
Preparation example 2:15% of a compound of formula I. Zhongshengmycin suspension (4
12g of a compound original drug shown in the formula I, 3g of a zhongshengmycin original drug, 1g of fatty alcohol-polyoxyethylene ether, 1g of an EO-PO block copolymer, 2g of lauryl sodium sulfate, 0.5g of an organic silicon defoamer, 1g of a thickening agent xanthan gum and 2.5g of an antifreeze glycol are added, deionized water is finally used for supplementing to 100g of the mixture, after the mixture is uniformly mixed, a sanding medium is added, a sand mill is used for high-speed grinding for 2 hours until D90 (the particle size of 90 percent of particles) is less than 10 mu m, and the obtained ground slurry is filtered out of the sanding medium to obtain the suspending agent with the mass content of the effective components of 15 percent.
Preparation example 3:4% Compound of formula I amino-oligosaccharin microemulsion (3
3g of original compound of the formula I, 1g of amino-oligosaccharin, 10g of dimethyl sulfoxide, 5g of calcium dodecyl benzene sulfonate and 2g of ethanol are fully dissolved and mixed to form a uniform and transparent oil phase, deionized water is added to complement 100g, and the uniform and transparent solution is formed by slight stirring, so that the microemulsion with the mass content of the effective components being 4wt% is prepared.
Preparation example 4:30% compound of formula I pyraclostrobin emulsifiable concentrate (1
10g of compound original drug of the formula I, 20g of pyraclostrobin, 2g of calcium dodecyl benzene sulfonate, 8g of castor oil polyoxyethylene ether and 100g of 200# solvent oil are added, and the materials are mixed, stirred and completely dissolved.
Preparation example 5:60% of the compound of formula I-bronopol water dispersible granule (2
40g of a compound raw material medicine of a formula I, 20g of a bronopol raw material medicine, 2g of lauryl sodium sulfate, 3g of ethoxylate phosphate, 2g of sodium lignosulfonate, 5g of white carbon black and 100g of filler bentonite are added. The white carbon black is firstly used for adsorbing the technical material shown in the formula I, and the materials are uniformly mixed, pulverized and uniformly mixed through airflow. And placing the crushed material in a beaker, and dropwise adding a binder aqueous solution containing 5g of soluble starch and 1g of urea while stirring until the mixture can be primarily kneaded into mud. And (4) putting the kneaded wet material into an extrusion granulator to extrude and granulate. And (3) drying the extruded wet granules in an oven at 54 ℃ to obtain the water dispersible granules with the effective component mass content of 60 wt%.
Preparation example 6:25% wettable powder of a compound of formula I zhongshengmycin (4
20g of a compound raw drug of a formula I, 5g of a zhongshengmycin raw drug, 5g of sodium lignosulphonate, 3g of sodium dodecyl sulfate, 1g of sodium chloride and 100g of attapulgite are complemented, pre-crushed and mixed uniformly, and then crushed to the fineness meeting the requirement that at least 98wt% passes through a 45-micron test sieve by a jet mill to prepare wettable powder with the mass content of an effective component of 25 wt%.
Preparation example 7:9% Compound-amino-oligosaccharin aqueous emulsion of formula I (2
6g of a compound of a formula I, 802.7g of castor oil polyoxyethylene ether EL-802, 0.3g of calcium dodecyl benzene sulfonate, 1g of glycerol, 10g of 200# solvent oil, 3g of amino-oligosaccharin and distilled water are added to make up to 100g, and the mixture is uniformly stirred and then sheared for 5min at 5000r/min to prepare an aqueous emulsion with the mass content of active ingredients of 9%.
Preparation example 8:30% dispersible oil suspension of the compound of formula I pyraclostrobin (5
25g of a compound shown in the formula I, 5g of pyraclostrobin, 4g of calcium dodecyl benzene sulfonate, 10g of nonylphenol polyether phosphate, 5g of phenethylphenol polyoxyethylene polyoxypropylene ether, 2g of magnesium aluminum silicate and 100g of methyl esterified soybean oil. Putting the materials into a mixing kettle, stirring and mixing, then passing through a colloid mill, then entering a sand mill for three-stage sanding, and finally uniformly shearing in a shearing machine to obtain the dispersible oil suspending agent with the mass content of the effective components of 30 wt%.
In order to enhance the dispersibility, emulsibility, wettability, stability, permeability and the like of the composition, the composition is added with solvents or cosolvents, emulsifiers, dispersants, coemulsifiers, antifreezes, antifoaming agents, thickeners, stabilizers or binders with different weights and processed into different formulations according to the processing method known by the technical personnel. The preparation can be diluted into a water dispersible solution by adding water, and is applied to different crops and occasions to prevent and control agricultural diseases by adopting modes of spraying, atomizing, powder spraying, broadcasting, irrigating or root irrigation and the like.
Indoor toxicity test
Experiment of bacterial inhibition Effect
The experiment adopts an indoor toxicity determination method. Indoor toxicity determination shows that the Synergistic Ratio (SR) of the two medicaments compounded according to a certain proportion is definite, the SR < 0.5 is antagonism, the SR < 0.5 is not less than 1.5 is additive action, and the SR > 1.5 is synergistic action.
The method for measuring indoor virulence of bacteria comprises the following steps: according to the agricultural industry standard of the people's republic of China (NY/T1156.16-2008), the bacteria growth inhibition amount test turbidity method is adopted for determination. Adding appropriate amount of medicinal liquid into cooled NB culture medium according to experimental design concentration, repeating for 4 times, and diluting the strain grown on NA culture medium to 1 × 10 with sterilized water 7 And (3) inoculating 100uL of bacterial liquid into each treatment culture medium for each spore/mL suspension, and shaking at 28-30 ℃ (120 r/min).
The turbidity of each treatment was measured before the start of the incubation, and when the control treatment reached the logarithmic phase, the turbidity of each treatment was measured and recorded. According to the survey data, the calculation formula is shown as formula (1) to calculate the bacterial growth inhibition rate.
Carrying out regression analysis on the logarithm value and the control effect rate value of the concentration of the medicament by using SPSS (Spassmex Sublimata), and counting EC (effective dose) of each treatment 50 And calculating the EC of each agent therefrom 50 The value is obtained. Meanwhile, the combined Synergistic Ratio (SR) of the two medicaments in different proportions is calculated according to a Wadley method, and the calculation formulas are shown as formula (2) and formula (3): SR = EC 50 (theoretical value)/EC 50 (found value, formula (2), EC 50 (theoretical value) = (a + b)/[ (a/(EC of A)) 50 ) + (EC of B/B 50 )]Formula (3), wherein: a. b is the proportion of active components A and B in the composition, A is a compound I, and B is selected from one of bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin.
Example 1
Test germs: ralstonia solanacearum (ralstonia solanacearum, guangdong collection of microorganisms); and (3) experimental design: the effective inhibition concentration range of the compound A (the compound of the formula I) and the compound B (the bronopol, the zhongshengmycin, the amino-oligosaccharin and the pyraclostrobin) and the mixture of the compound A and the compound B in different proportions is determined through a preliminary test. The virulence determination results are shown in tables 1-4.
TABLE 1 Joint virulence determination of tomato ralstonia solanacearum by different proportions of compounds of formula I and bronopol
The indoor activity test result shows that the compound of the formula IAnd bronopol as single agents EC 50 The content ratio of the effective components of the two medicaments is 10, 1, 5, 1,2 and 1, and the synergistic coefficients are 1.75, 1.84, 2.90 and 3.09: the compound ralstonia solanacearum under 1 has better inhibition effect.
TABLE 2 Combined virulence determination of L.solani with different ratios of compounds of formula I and Zhongshengmycin
The indoor activity measurement result shows that the compound of the formula I and the zhongshengmycin are EC single agents 50 16.57mg/L and 29.46mg/L respectively, the synergistic coefficients of the two medicaments are respectively 2.05, 2.73, 1.69 and 1.60, and the content ratio of the effective components of the two medicaments is 8: the compound ralstonia solanacearum under 1 has better inhibition effect.
TABLE 3 measurement of the Combined virulence of L.solani for compounds of formula I and amino-oligosaccharins in different ratios
The indoor activity measurement result shows that the compound of the formula I and the amino-oligosaccharin have two single agents EC 50 The content ratio of the effective components of the two medicaments is respectively 16.57mg/L and 47.41mg/L, the content ratio of the effective components of the two medicaments is 5:1, the composite paraquat Laurella has better inhibition effect.
TABLE 4 determination of the Combined virulence of the Compound of formula I and pyraclostrobin at different ratios for L.solani
The indoor activity test result shows that the compound shown in the formula I and the pyraclostrobin have two single-dose EC 50 The effective component content ratios of the two medicaments are respectively 16.57mg/L and 36.26mg/L, and the effective component content ratios of the two medicaments are respectively 1.76, 2.23 and 1.87, wherein the two medicaments have synergistic effects, and the compound in the formula I and the pyraclostrobin have better inhibition effect on the bacterial wilt bacterium under the condition of 1.
Experiment of fungus inhibitory Effect
The method for measuring the indoor toxicity of the fungi adopts a hypha growth rate method to measure the control effect of the medicament according to the agricultural industry standard (NY/T1156.2-2008) of the people's republic of China. The pharmaceutical composition provided by the invention is used for testing the inhibition effect on fusarium oxysporum. The cultured fusarium oxysporum is cultured by using a sterilization puncher with the diameter of 7mm under the aseptic operation condition, bacterial colonies with consistent growth vigor are selected, bacterial cakes are cut from the edges of the bacterial colonies, the bacterial cakes are inoculated to the center of a drug-containing flat plate by using an inoculator, hypha faces downwards, a dish cover is covered, and the medicine-containing flat plate is placed in an incubator at 25 ℃ for dark culture. And (3) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the bacterial colony in the blank control culture dish, measuring the diameter of each treated bacterial colony by a cross method after the bacterial colony in the blank control grows sufficiently, calculating the growth diameter of the bacterial colony by adopting a formula (4), and taking the average value of the growth diameters.
Colony growth diameter = colony diameter-cake diameter (4)
The inhibition rate is calculated by formula (5), and the inhibition rate of each medicament treatment on the growth of fusarium oxysporum hyphae is calculated according to the growth diameter of the blank control colony and the growth diameter of the medicament treatment colony.
Carrying out regression analysis on the logarithm value and the control effect rate value of the concentration of the medicament by using SPSS (Spassmex Sublimata), and counting EC (effective dose) of each treatment 50 And is composed ofThis calculates the EC50 value for each agent. The calculation of the synergistic effect is the same as the experiment of the bacteria inhibition effect.
Example 2
Test germs: fusarium oxysporum (Fusarium oxysporum XY-WSW-1230, shanghai Xuan Yay Biotech Co., ltd.); and (3) experimental design: the effective inhibition concentration range of the compound A (compound shown in formula I) and the compound B (bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin) and the mixture of the compound A and the compound B in different proportions is determined through a preliminary test. The virulence determination results are shown in tables 5-8.
TABLE 5 Joint virulence determination of Fusarium oxysporum with different ratios of the compound of formula I to bronopol
The indoor activity measurement result shows that the compound of the formula I and the bronopol have two single agents EC 50 The compound is 125.23mg/L and 2568.65mg/L respectively, the content ratio of the effective components of the two medicaments is 2.36, 1,2, the coefficient of synergy is 2.36, 2.01 and 1.57, and the two medicaments both have synergy, wherein the compound of the formula I and the bronopol have better inhibition effect on fusarium oxysporum under the condition that the ratio is 2.
TABLE 6 Combined virulence determination of Fusarium oxysporum for different ratios of compounds of formula I to zhongshengmycin
The indoor activity test result shows that the compound of the formula I and the zhongshengmycin are two single agents EC 50 The two medicaments are 125.23mg/L and 512.61mg/L respectively, the content ratios of the effective components of the two medicaments are respectively 16, 1, 8, 1, 4 and 1, the synergistic coefficients are respectively 1.69, 2.35, 2.66 and 2.07, and the two medicaments have synergistic effects, wherein the compound of the formula I and the bronopol have the synergistic effect on the fusarium oxysporum under the condition of 4Better inhibiting effect.
TABLE 7 Joint virulence determination of Fusarium oxysporum with different ratios of compounds of formula I to amino-oligosaccharins
The indoor activity measurement result shows that the compound of the formula I and the amino-oligosaccharin have two single agents EC 50 The content ratio of the effective components of the two medicaments is respectively 125.23mg/L and 509.70mg/L, the synergistic coefficients of 1, 3 and 1 are respectively 2.25, 2.77 and 1.71, and the two medicaments both have synergistic effect, wherein the compound of the formula I and the amino-oligosaccharin have better inhibiting effect on fusarium oxysporum under the condition of 3.
TABLE 8 Joint virulence determination of Fusarium oxysporum by different ratios of the compound of formula I to pyraclostrobin
The indoor activity measurement result shows that the compound of the formula I and the pyraclostrobin have two single agents EC 50 The compound is 125.23mg/L and 376.04mg/L respectively, the content ratio of the effective components of the two medicaments is 15, 1, 7, 1,2 and 1, the following coefficients are 1.67, 1.88, 2.55, 2.35 and 1.79 respectively, and the two medicaments both have synergistic action, wherein the compound of the formula I and the pyraclostrobin have better inhibiting effect on fusarium oxysporum under the condition of 5.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the relevant art can make various changes and modifications without departing from the spirit and scope of the invention, and therefore all equivalent technical solutions also belong to the scope of the invention.
Those not described in detail in this specification are within the skill of the art.
Claims (8)
1. A sterilization composition is characterized in that active ingredients of the sterilization composition consist of an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound shown in a formula I, and the active ingredient B is any one of bronopol, zhongshengmycin, amino-oligosaccharin and pyraclostrobin;
the mass ratio of the active ingredient A to the bronopol is (10);
the mass ratio of the active ingredient A to the zhongshengmycin is 16;
the mass ratio of the active component A to the amino-oligosaccharin is (5);
the mass ratio of the active ingredient A to the pyraclostrobin is 15-1;
the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 3-60 wt% based on the total weight of the bactericidal composition being 100 wt%;
the bactericidal composition also comprises auxiliary materials, wherein the auxiliary materials are at least one of emulsifying agents, dispersing agents, wetting agents, spreading agents, stabilizing agents, defoaming agents, synergists, sticking agents, safety agents, thickening agents, disintegrating agents, antifreezing agents, solvents, preservatives or fillers.
2. The use of the fungicidal composition of claim 1 for inhibiting ralstonia solanacearum, characterized in that:
the mass ratio of the active ingredient A to the bronopol is 10-1;
the mass ratio of the active component A to the zhongshengmycin is (8);
the mass ratio of the active component A to the amino-oligosaccharin is 5;
the mass ratio of the active ingredient A to the pyraclostrobin is 5.
3. The use of the fungicidal composition of claim 1 for the inhibition of fusarium oxysporum, wherein:
the mass ratio of the active ingredient A to the bronopol is 2;
the mass ratio of the active ingredient A to the zhongshengmycin is 16-1;
the mass ratio of the active component A to the amino-oligosaccharin is (5);
the mass ratio of the active ingredient A to the pyraclostrobin is 15-1.
4. A formulation prepared from the germicidal composition of claim 1, wherein: the preparation formulation is selected from any one of wettable powder, granules, soluble powder, soluble tablets, powder, water dispersible tablets, dispersible liquid, emulsifiable concentrate, aqueous emulsion, microemulsion, suspending agent, dispersible oil suspending agent, soluble liquid and ultra-low volume liquid.
5. A formulation prepared from the germicidal composition of claim 1, wherein: the preparation formulation is water dispersible granule or microcapsule granule.
6. A formulation prepared from the germicidal composition of claim 1, wherein: the preparation is in the form of microcapsule suspension.
7. Use of a formulation according to any one of claims 4 to 6 for inhibiting Lawsonia solani, characterized in that:
the mass ratio of the active ingredient A to the bronopol is 10-1;
the mass ratio of the active component A to the zhongshengmycin is (8);
the mass ratio of the active component A to the amino-oligosaccharin is 5;
the mass ratio of the active ingredient A to the pyraclostrobin is 5.
8. Use of a formulation according to any one of claims 4 to 6 for inhibiting fusarium oxysporum, wherein:
the mass ratio of the active ingredient A to the bronopol is (2);
the mass ratio of the active component A to the zhongshengmycin is (16);
the mass ratio of the active ingredient A to the amino-oligosaccharin is 5-1;
the mass ratio of the active ingredient A to the pyraclostrobin is 15-1.
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