CN114302731A - 用于治疗疾病的外泌体 - Google Patents
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120437174A (zh) * | 2025-02-28 | 2025-08-08 | 单核细胞治疗有限公司 | 脐带血外泌体在制备治疗衰老引发的眼科疾病的药物中的应用 |
| CN120927522A (zh) * | 2025-10-14 | 2025-11-11 | 亿航(苏州)生物医药有限公司 | 辅助诊断或鉴别血管性认知障碍的标志物、试剂盒及用途 |
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| EP3801028A4 (en) | 2018-05-30 | 2022-04-13 | Direct Biologics LLC | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
| US12569517B2 (en) | 2019-02-07 | 2026-03-10 | Direct Biologics, Llc | Method for treating osteoarthritis with mesenchymal stem cell exosomes |
| US12213995B2 (en) | 2019-07-18 | 2025-02-04 | Direct Biologics, Llc | Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use |
| US20210322483A1 (en) | 2020-04-16 | 2021-10-21 | Ichilov Tech Ltd. | Cell-derived particles presenting heterologous cd24 and use thereof in therapy |
| US20210324339A1 (en) | 2020-04-16 | 2021-10-21 | Ichilov Tech Ltd. | Cell-derived particles presenting heterologous cd24 and use thereof in therapy |
| KR20230004709A (ko) | 2020-04-22 | 2023-01-06 | 다이렉트 바이오로직스 엘엘씨 | 감염성 질환과 연관된 염증성 병태를 치료하기 위한 방법 및 조성물 |
| KR102317052B1 (ko) * | 2020-05-04 | 2021-10-25 | 주식회사 티에스셀바이오 | 태반 유래의 세포외 소포의 항염증 항바이러스 효과 조성물 |
| WO2022154149A1 (ko) * | 2021-01-14 | 2022-07-21 | (주)엑솔런스 | 세포외소포체를 이용한 백신 조성물 |
| WO2022183047A1 (en) * | 2021-02-26 | 2022-09-01 | The Methodist Hospital | Regulatory t cell (treg) extracellular vesicle compositions and methods |
| CN113171379A (zh) * | 2021-04-28 | 2021-07-27 | 奥启(深圳)创投科技有限公司 | 一种间充质干细胞外泌体在制备脂肪性肝病药物中的应用 |
| CN114085817B (zh) * | 2021-11-26 | 2025-04-15 | 三代康年(上海)医疗科技有限公司 | 一种包载microRNA的外泌体的制备方法及其产品和应用 |
| CN119013038A (zh) * | 2022-04-08 | 2024-11-22 | 创新外科再生控股有限公司 | 用于伤口愈合和促进毛发生长和/或再生的nampt |
| CN117414415B (zh) * | 2023-07-10 | 2024-10-18 | 中山大学·深圳 | 蛋白质精氨酸甲基转移酶7在促进毛发生长中的应用 |
| WO2025048567A1 (ko) * | 2023-08-31 | 2025-03-06 | 의료법인 성광의료재단 | 인간 태반 유래 중간엽 줄기 세포에서 분리된 엑소좀을 포함하는 시신경 질환 및 미토콘드리아 기능이상 질환 예방 또는 치료용 약학적 조성물 |
| KR20250139693A (ko) * | 2024-03-15 | 2025-09-23 | 가톨릭대학교 산학협력단 | Ssea3 및 cd105를 발현하는 신경능 유래 코 줄기세포 유효성분으로 포함하는 알츠하이머병 예방 또는 치료용 약학적 조성물 |
| US12502407B2 (en) | 2024-04-25 | 2025-12-23 | Direct Biologics, Llc | Treatment of fistula with bone marrow mesenchymal stem cell derived extracellular vesicles |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861430A (zh) * | 2016-04-29 | 2016-08-17 | 南京大学 | 一种外泌体、外泌体的制备方法及其在制备治疗脓毒症药物或者制剂中的应用 |
| WO2018083700A1 (en) * | 2016-11-03 | 2018-05-11 | Exostem Biotec Ltd. | Mesenchymal stem cells populations, their products, and use thereof |
| CN108366567A (zh) * | 2015-05-26 | 2018-08-03 | 细胞结构公司 | 使用受刺激的胎盘干细胞的血管生成 |
| WO2019099955A1 (en) * | 2017-11-16 | 2019-05-23 | Celularity, Inc. | Cultivation of placenta to isolate exosomes |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1969147B1 (en) * | 2006-01-05 | 2014-07-30 | The Ohio State University Research Foundation | microRNA-based methods for the diagnosis of stomach cancer |
| EP3115469B1 (en) * | 2007-11-19 | 2020-04-29 | Celera Corporation | Lung cancer markers and uses thereof |
| CN102307475A (zh) * | 2009-02-04 | 2012-01-04 | 彼帕科学公司 | 用与生长因子抑制剂组合的parp抑制剂治疗肺癌 |
| WO2016057755A1 (en) * | 2014-10-09 | 2016-04-14 | Anthrogenesis Corporation | Placenta-derived adherent cell exosomes and uses thereof |
-
2020
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2021
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108366567A (zh) * | 2015-05-26 | 2018-08-03 | 细胞结构公司 | 使用受刺激的胎盘干细胞的血管生成 |
| CN105861430A (zh) * | 2016-04-29 | 2016-08-17 | 南京大学 | 一种外泌体、外泌体的制备方法及其在制备治疗脓毒症药物或者制剂中的应用 |
| WO2018083700A1 (en) * | 2016-11-03 | 2018-05-11 | Exostem Biotec Ltd. | Mesenchymal stem cells populations, their products, and use thereof |
| WO2019099955A1 (en) * | 2017-11-16 | 2019-05-23 | Celularity, Inc. | Cultivation of placenta to isolate exosomes |
Non-Patent Citations (1)
| Title |
|---|
| 赵丽华 等: "exosome 及其在免疫耐受方面的作用", 生命科学, vol. 19, no. 2, 30 April 2007 (2007-04-30), pages 174 - 178 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120437174A (zh) * | 2025-02-28 | 2025-08-08 | 单核细胞治疗有限公司 | 脐带血外泌体在制备治疗衰老引发的眼科疾病的药物中的应用 |
| CN120927522A (zh) * | 2025-10-14 | 2025-11-11 | 亿航(苏州)生物医药有限公司 | 辅助诊断或鉴别血管性认知障碍的标志物、试剂盒及用途 |
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| ZA202109828B (en) | 2024-04-24 |
| KR20220024060A (ko) | 2022-03-03 |
| WO2020257720A1 (en) | 2020-12-24 |
| BR112021025512A2 (pt) | 2022-02-01 |
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