Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, which will enable those skilled in the art to practice the present invention with reference to the accompanying specification. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, and the like are used based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the dimension from top to bottom, "width" corresponds to the dimension from left to right, and "depth" corresponds to the dimension from front to back. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms concerning attachments, coupling and the like (e.g., "connected" and "attached") refer to a relationship wherein structures are secured or attached, either directly or indirectly, to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
Example 1
The present invention provides a chemiluminescent immunoassay analyzer, as shown in fig. 1, 4, 5, 6, 13, comprising an analysis device 100, the analysis device 100 comprising:
a bracket 11 having a light-shielding structure and provided with a plurality of mounting grooves 111 for mounting the reaction cups 12;
a heating device 13 including a heating base 131 which is a light shielding structure; the heating seat 131 is provided with a plurality of cavities 1311; the cavity 1311 corresponds to the mounting groove 111; one side of the cavity 1311 is opened to form a light outlet 1313;
a light shielding shell 14 having a hollow cavity 141 for enclosing the sample transfer tube 70 during filling;
the photon counting device 16 is provided with a light inlet and is used for detecting a light-emitting signal of a sample to be detected in the reaction cup 12;
wherein, the shading shell 14 covers the periphery of the contour of the installation groove 111 to form a shading sample adding channel together; the bracket 11 is installed on the heating seat 131 and covers a plurality of the cavities 1311 to form a light-shielding structure of the mouth of the reaction cup 12; the light outlet 1313 is matched with the light inlet to form a light-shading light outlet channel.
In this embodiment, a light-shielding sample feeding channel is formed by matching the light-shielding shell 14 with the bracket 11; a light-shielding structure surrounding the reaction cup 12 is formed by the mounting groove 111 of the bracket 11 and the cavity 1311 of the heating seat 131; light outlet 1313 through heating seat 131 and the income light mouth of photon counting assembly 16 cooperate and form the light-emitting channel of light-tight, and then realize from the application of sample to the light-tight environment that detects whole process, replace the tradition and additionally set up the scheme of the light-tight room of big cover, realize strictly avoiding light and be favorable to whole analytical equipment 100's miniaturized design when improving and detect the accuracy, simple structure, small and exquisite, easy and simple to handle, improvement detection speed.
In one embodiment, as shown in fig. 4, the cavities 1311 are uniformly arranged in a row; the light outlets of the cavities 1311 are oriented in the same direction, so that the light outlets 1313 at different positions on the heating seat 131 can be aligned with the light inlets of the photon counting device 16.
In one embodiment, as shown in fig. 10, the photon counting device 16 comprises a photon counter 161, a first driving component 163; the first driving component 163 is used for driving the photon counter 161 to reciprocate along the Y direction to approach or move away from the light outlet 1313;
as shown in fig. 4, the heating device 13 further includes a second driving assembly 132 connected to the heating base 131 for driving the heating base 131 to reciprocate along the X direction to switch different light outlets 1313 to align with the light inlets. Specifically, the second driving assembly 132 is arranged, on one hand, the second driving assembly 132 drives the heating base 131 to reciprocate so as to agitate and mix the substance in the reaction cup 12 in which the heating base 131 is arranged; on the other hand, the movement of the heating base 131 is matched with the movement of the photon counter 161 driven by the second driving assembly 132, so as to align the light outlet 1313 and the light inlet corresponding to different reaction cups 12.
Further, as shown in fig. 1, the cleaning device 17 is further included, and includes a plurality of cleaning needle groups 171, a plurality of magnets 172; the cleaning needle group 171 comprises a waste extracting needle 1711 and a cleaning needle 1712; wherein,
the cleaning needle group 171 corresponds to the magnets 172 in position one by one;
the plurality of cleaning needle sets 171 are uniformly arranged in a row along the moving direction of the heating device 13 to correspond to one of the reaction cups 12, respectively. Specifically, a washing device 17 is provided to improve the automation of the immunoassay. The cleaning needle 1712 is used to fill the reaction cuvette 12 with a cleaning solution to clean the reaction cuvette 12. The waste extraction needle 1711 is used to extract waste liquid from the reaction cup 12. The magnet 172 is used to attract the magnetic particles in the reaction cup 12 before the waste liquid is pumped out, so as to prevent the magnetic particles from being attracted away, on one hand, the magnetic particles still need to be left in the reaction cup 12 to participate in the subsequent reaction step, and on the other hand, the magnetic particles are prevented from blocking the waste needle. In addition, the cleaning needle sets 171 are arranged in a structural design, and the reaction cup 12 moves along the Y direction along with the heating base 131 under the driving of the second driving assembly 132, so as to be away from or aligned with the corresponding cleaning needle set 171, thereby simplifying the structural design of the cleaning device 17.
Further, the plurality of magnets 172 are fixed to the photon counting device 16, so as to omit an additional fixing structure for installing the magnets 172, simplify the structure, reduce the occupied space, and facilitate the miniaturization design of the analysis apparatus 100. Further, when the magnet 172 is attracted, the second driving component 132 drives the heating base 131 to move, so that after the magnet 172 faces the reaction cup 12 to be cleaned, the first driving component 163 drives the magnet 172 to be inserted into the light outlet 1313 of the heating base 131 to be close to the body of the reaction cup 12 in the cavity 1311, thereby achieving attraction. That is, the light outlet 1313 forms a light outlet channel for the substance in the reaction cup 12, and forms a magnet attraction channel.
Further, as shown in fig. 1, the cleaning device 17 further includes a third driving assembly 173 connected to the plurality of cleaning needle sets 171 for driving the cleaning needle sets 171 to reciprocate along the Z direction. Specifically, in order to facilitate the extraction of waste liquid and the filling of the cleaning liquid, the third driving assembly 173 is provided so that the waste extraction needle 1711 and the cleaning needle 1712 can be inserted into the reaction cup 12. Cleaning device 17, heating seat 131, photon counter 161 only make reciprocating motion along one direction respectively, mutually support to simplify the overall structure's of each part complexity, be convenient for assemble and arrange regularly, effectively utilize the space in the analytical equipment 100.
In one embodiment, as shown in FIG. 1, the cleaning device 17 includes a first mounting seat 174; the third driving assembly 173 includes a synchronous belt transmission mechanism 1731, a second slide rail 1732, a third adapting block 1733, and a needle set fixing plate 1734; the cleaning pin sets 171 are fixed to the pin set fixing plate 1734, and the pin set fixing plate 1734 is fixed to the third adapting block 1733; one side of the third adapting block 1733 is slidably connected to the second sliding rail 1732, and the other side is connected to the synchronous belt transmission mechanism 1731. The synchronous belt transmission mechanism 1731 moves along the Z direction to drive the third adapting block 1733 to drive the needle set fixing plate 1734 to move, so that the cleaning needle set 171 moves along the Z direction. The second rail 1732 serves as a guide and support to stabilize the movement of the cleaning pin assembly 171.
In one embodiment, the number of the cleaning needles 1712 is two, and the cleaning needles are used for filling with an acidic cleaning solution and an alkaline cleaning solution.
In one embodiment, as shown in fig. 2, 3 and 5, the apparatus further comprises a sampling device 18, wherein the sampling device 18 comprises a light shield 181, an assembling head 182; the assembling head 182 is arranged at the bottom of the light shield 181 and used for assembling and disassembling the sample transferring tube 70; the sample transfer tube 70 extends into the hollow cavity 141, and the light shield 181 covers the opening above the hollow cavity 141 to close the sample adding channel. Specifically, the light shield 181 has a cavity therein for accommodating mechanical components for forming sampling power and sample-adding power; the bottom of the light shield 181 is provided with a yielding notch for yielding the assembling head 182 of the internal sampling and sample adding component thereof to stretch out. The light shield 181 is matched with the light shielding shell 14 to close the upper opening of the hollow cavity 141, so as to form a closed sample feeding channel.
Further, the sampling device 18 includes an X-direction driving component, a Y-direction driving component, and a Z-direction driving component, so as to enable the light shield 181 and the assembling head 182 to realize three-dimensional movement.
In an embodiment, as shown in fig. 3, 5, 6, 7, and 9, the light shielding shell 14 further includes a cylinder 142, a cover 143, and a spring 144; the top end and the bottom end of the cylinder 142 are respectively used for abutting against the light shield 181 and the periphery of the contour of the mounting groove 111; the cover 143 is sleeved on the end of the cylinder 142 facing the reaction cup 12;
the top of the housing 143 is fixed on the fixing plate 151 in the analysis device, and the bottom end of the housing is provided with a first abutting part 1431; a second abutting part 1421 is convexly arranged on the periphery of the cylinder 142; the first abutting portion 1431 and the second abutting portion 1421 form a clamping area 145 together; the spring 144 is disposed in the clamping region 145, and two ends of the spring are respectively abutted against the first abutting portion 1431 and the second abutting portion 1421; a gap is left between the upper surface of the second abutting portion 1421 and the fixing plate 151. Specifically, the top of the housing 143 is fixed by the fixing plate 151, and the housing 143 is fixed. Since the spring 144 is disposed between the first abutting portion 1431 and the second abutting portion 1421, when the top end and the bottom end of the light shielding shell 14 abut against the light shielding cover 181 and the contour outer periphery of the mounting groove 111, respectively, the second abutting portion 1421 presses the spring 144 downward due to the light shielding cover 181 pressing the light shielding shell 14 downward, and the spring 144 is compressed and deformed. When one cuvette 12 is loaded and tested, the sampling device moves the sample transfer tube 70 away, the light shield 181 removes the pressing on the top end of the cylinder 142, the spring 144 rebounds to push the second abutting part 1421 upwards, so that the bottom end of the cylinder 142 moves away from the opening of the cuvette 12, i.e., the bottom end of the cylinder 142 is separated from the abutting contact with the contour of the mounting groove 111, i.e., the cylinder 142 is separated from the abutting contact with the bracket 11, and the bracket 11 moves along with the heating seat 131 to switch the next cuvette 12 for loading and testing.
Further, the spring 144 is sleeved outside the cylinder 142, so that the assembly is simple, and the spring 144 surrounds the cylinder 142 to uniformly distribute the acting force of the spring 144 on the first abutting portion 1431 and the second abutting portion 1421.
In one embodiment, as shown in fig. 6, the housing 143 has a plurality of first mounting holes 1432 for engaging fasteners to be fixed to the fixing plate 151, so that the installation is convenient.
In one embodiment, as shown in fig. 5 and 6, the top peripheral side surface of the housing 143 abuts against the peripheral side surface of the third groove 1511 on the lower surface of the fixing plate 151; the cylinder 142 passes through the through groove 1512 of the fixing plate 151 from below the fixing plate 151. Specifically, a third groove 1511 is formed by recessing the lower surface of the fixing plate 151, the third groove 1511 is provided with a through groove 1512, and the cylinder 142 is inserted from below the through groove 1512 until the top end of the housing 143 abuts against the third groove 1511. The circumferential surface of the third groove 1511 abuts against the circumferential surface of the housing 143, so that the housing 143 can be positioned and mounted relative to the fixing plate 151, and the mounting is facilitated.
Further, the outer peripheral surface of the cylinder 142 abuts against the inner surface of the through slot 1512. The barrel 142 is retained and guided by the inner surface of the channel 1512, i.e., the barrel 142 moves along the channel 1512 as the barrel 142 moves upward under the spring 144 rebounds.
In one embodiment, as shown in fig. 3, 6 and 9, the cylinder 142 includes, from top to bottom, a first cylinder 1422 and a second cylinder 1423; the second abutting portion 1421 is disposed on the first cylinder 1422; the second cylinder 1423 is disposed on the bottom wall of the second abutting portion 1421. Specifically, through the structural design of the cylinder 142, a structure with a narrow top and a wide bottom is formed, and the wide bottom structure is used for facilitating the bottom end of the cylinder 142 to cover the contour periphery of the installation groove 111. The narrow-top structure is used to reduce the space occupied by the cylinder 142, which is beneficial to the miniaturization design of the analysis apparatus 100.
In one embodiment, as shown in fig. 10-13, the photon counting apparatus 16 further comprises a shutter assembly 162; the shutter assembly 162 includes a light blocking housing, a baffle 1624, a fourth drive assembly 1625; the light shielding shell is provided with a cavity 1621, a first light through port 16221 and a second light through port 16231 which are communicated; the first light passing port 16221 corresponds to the second light passing port 16231 and the position of the detection head 1611 of the photon counter 161 respectively; the second light-passing port 16231 is matched with the light-emitting port 1313 to form a light-emitting channel of the reaction cup 12;
the baffle 1624 is rotatably disposed in the cavity 1621; the fourth driving component 1625 drives the baffle 1624 to rotate so as to connect or disconnect the optical path formed by the first light passing port 16221 and the second light passing port 16231. Specifically, the shutter member 162 is provided at the detection end face of the photon counter 161, forming a good light-shielding environment for the detection head 1611 of the photon counter 161. The shutter assembly 162 includes a light shielding case, the light shielding case covers the contour periphery of the detection head 1611, when the photon counting device 16 is in an undetected state, the baffle 1624 in the light shielding case covers the first light passing port 16221 and/or the second light passing port 16231 to disconnect the light path formed by the first light passing port 16221 and the second light passing port 16231, at this time, light outside the photon counting device 16 is blocked by the baffle 1624, and the light is prevented from contacting the detection head 1611, so that the performance of the electronic element in the photon counter 161 is prevented from being influenced by ambient light. During detection, the second light passing port 16231 is aligned with the light outlet 1313, the baffle 1624 is rotated, a light path formed by the first light passing port 16221 and the second light passing port 16231 is conducted, and a light emitting signal of a sample to be detected is received, so that detection is realized. The shutter assembly 162 is simple and small in structure and easy and convenient to operate, provides a light-shielding environment for the photon counter 161, and improves the detection accuracy of the photon counter 161.
In one embodiment, the light-shielding housing includes a first housing 1622, a second housing 1623; the first housing 1622 and the second housing 1623 are fixed to form the cavity 1621; the first light-passing port 16221 and the second light-passing port 16231 are disposed on the first housing 1622 and the second housing 1623, respectively. Specifically, through the split structure design of the first housing 1622 and the second housing 1623, on the one hand, the processing and the forming are facilitated, and on the other hand, the assembling and disassembling of the baffle 1624 are facilitated. When the baffle 1624 has a position error due to a large number of rotations, the second housing 1623 may be detached, and the baffle 1624 may be adjusted or replaced.
Further, as shown in fig. 11 and 12, the first housing 1622 is provided with a protruding portion 16222, and the second housing 1623 is provided with a fifth card slot 16232; the protrusion 16222 is snapped into the fifth snap groove 16232 to form a closed structure around the cavity 1621. Specifically, the protruding portion 16222 and the fifth card slot 16232 are assembled around the cavity 1621, and the card assembly structure improves the light shielding property at the assembly position to prevent the photon counter 161 from being affected by ambient light and improve the detection accuracy.
Further, the first housing 1622 and the second housing 1623 are fixed by a fastener to secure the assembly of the first housing 1622 and the second housing 1623.
In one embodiment, as shown in fig. 10 to 13, the baffle 1624 is provided with a third light-passing port 16241, a first rib 16242, and a second rib 16243; the first rib 16242 and the second rib 16243 respectively surround the outer contours of two sides of the third light-passing port 16241 to form a first groove 16244 and a second groove 16245; the third light passing port 16241 is used for communicating the first light passing port 16221 with the second light passing port 16231;
the first light passing port 16221 is formed by extending towards the baffle 1624 to form a first flange 16223, and the second light passing port 16231 is formed by extending towards the baffle 1624 to form a second flange 16233; during movement of the baffle 1624, the first groove 16244 moves around the first rim 16223 and the second groove 16245 moves around the second rim 16233. Specifically, because the light transmission in-process can take place the scattering, baffle 1624 sets up third light passing port 16241 for the light path of first light passing port 16221, second light passing port 16231 is restricted by third light passing port 16241, and third light passing port 16241 plays the effect of guide light trend promptly, and the light that the detection sample that second light passing port 16231 accepted is concentrated and is transmitted to first light passing port 16221 through third light passing port 16241, improves and detects the accuracy. Further, a first rib 16242 is provided on a surface of the baffle 1624 facing the photon counter 161, and a second rib 16243 is provided on a surface thereof facing the second housing 1623. The second rib 16243 is used to reduce the space of the second flange 16233, i.e., the baffle 1624 is provided with the second rib 16243 for collecting the light emitted from the second light-passing port 16231 to the third light-passing port 16241; the first rib 16242 is used to reduce the size of the space where the first flange 16223 is located, i.e., the baffle 1624 is provided with the first rib 16242 to collect the light emitted from the third light-passing port 16241 to the first light-passing port 16221, so as to improve the detection accuracy.
Further, the first light passing port 16221, the second light passing port 16231 and the third light passing port 16241 are all circular, so that the influence of the profile of the light passing ports on the light propagation path is reduced.
Further, the first light passing port 16221, the second light passing port 16231, and the third light passing port 16241 are the same in size so as to form a straight-through light propagation channel.
Further, the profile size of the detection head 1611 of the photon counter is larger than the first light passing port 16221, so as to reduce the loss of light transmitted from the first light passing port 16221 to the detection head 1611.
In one embodiment, as shown in fig. 10 to 13, the cavity 1621 is provided with a rotating shaft 1626, and the baffle 1624 is provided with a shaft hole 16246 to be rotatably connected to the rotating shaft 1626;
the fourth drive assembly 1625 includes:
a driving member 16251 fixed on the outer surface of the light shielding shell, wherein a driving shaft of the driving member extends into the cavity 1621 to form a rotary driving force;
a cam 16252 sleeved on the driving shaft of the driving member 16251;
and a shifting fork, one end of which is fixed to the cam 16252 and the other end of which is fixed to the baffle 1624 and is disposed near the rotating shaft 1626. Specifically, when the driving member 16251 operates, the driving shaft thereof drives the cam 16252 to move, the cam 16252 drives the shifting fork to move, and the shifting fork drives the baffle 1624 to rotate around the rotating shaft 1626. Through the mutual cooperation of cam 16252, shift fork, baffle 1624, pivot 1626, simple structure is small and exquisite, the running noise is little, the motion friction is little, the operation is stable, improves baffle 1624 and rotates the life-span, reduces the influence of rotation to baffle 1624 position.
Furthermore, the driving member 16251 is a rotary electromagnet, and is electrified to rotate to provide a rotary driving force, so that the structure is simple and small, and the miniaturization design of the photon counting device 16 is facilitated.
In one embodiment, as shown in fig. 10 and 11, the baffle 1624 has a fan shape; a second mounting hole 16247 is formed at the center of the circle of the baffle 1624 and used for mounting a shifting fork; the shaft hole 16246 of the baffle 1624 is arranged near the center angle of the circle; the connection line of the center of the shaft hole 16246 and the second mounting hole 16247 coincides with the center line of the baffle 1624; the third light passing opening 16241 is disposed eccentrically and away from the central angle. Specifically, through the structural design of the baffle 1624, the size of the baffle 1624 is reduced, the motion track of the third light passing port 16241 can be extended, and the baffle area on the peripheral side of the third light passing port 16241 is increased to improve the shielding effect on the first light passing port 16221 and/or the second light passing port 16231. That is, the overall size of the shutter assembly 162 is controlled while the shutter assembly 162 is made to provide the photon counter 161 with good light shielding performance.
In one embodiment, as shown in fig. 12, the shutter assembly 162 further includes a fixing seat 1627 embedded in the light shielding housing; the driving member 16251 is embedded in the fixing seat 1627; the rotating shaft 1626 sequentially penetrates through the shaft hole of the baffle 1624 and the first yielding hole 16224 of the shading shell and then is fixed on the fixed seat 1627. Specifically, a fixed mount 1627 is provided to facilitate installation of drive member 16251. The driving shaft of the driving member 16251 passes through the second escape hole 16225 of the light shielding housing and is coupled to the cam 16252. Design driving piece 16251 inlays locates fixing base 1627, and fixing base 1627 inlays locates the shading casing to increase the printing opacity degree of difficulty of fixing base 1627 and driving piece 16251 assembly department and increase the printing opacity degree of difficulty of fixing base 1627 and shading casing assembly department, further improve shutter subassembly 162's light shielding performance.
In one embodiment, as shown in fig. 10 to 13, the shutter assembly 162 further includes a light passing nozzle 1628 having a light passing channel 16281, the light passing channel 16281 being configured to form a light inlet of the photon counting device 16; one end of the light passage 16281 is connected to the second light passage 16231, and the other end of the light passage abuts against the outer peripheral surface of the profile on one side of the detection window. Specifically, the extending end 16282 formed by protruding the light passing nozzle 1628 abuts against the outer peripheral surface of the profile on one side of the detection window to form a closed light inlet channel together with the light outlet 1313 and the second light passing port 16231. Through setting up logical light mouth 1628, simplify the structural design of shading casing to reduce the accurate requirement of processing of shading casing.
Further, the light passing nozzle 1628 is embedded in the light shielding shell, so that the light transmitting difficulty at the assembling gap between the light passing nozzle 1628 and the light shielding shell is increased, and the light shielding performance is improved. In one embodiment, the light passing nozzle 1628 is embedded in the front surface of the second housing 1623.
In one embodiment, as shown in FIG. 10, the first drive assembly 163 is secured to the shutter housing of the shutter assembly 162.
In one embodiment, as shown in fig. 5 and 10, the first driving assembly 163 includes:
a first motor 1631 for providing a rotational driving force;
a screw 1632, one end of which is coaxially connected to the output shaft of the first motor 1631, and the other end of which is rotatably connected to the light-shielding housing;
a nut 1633 screwed to the screw rod 1632; the photon counter 161 is fixed to the nut 1633. Specifically, the first motor 1631 drives the lead screw 1632 to rotate, so that the nut 1633 makes a linear motion along the lead screw, and further drives the photon counter 161 to move along the extension direction of the lead screw to be far away from or close to the light outlet 1313. Further, the first driving assembly 163 further includes a second mounting seat 1634 for fixing the first motor 1631; the second mount 1634 is attached to the light shield housing.
Further, the first driving component 163 further includes a first transfer block 1635 for connecting the nut 1633 and the light shielding shell respectively, so as to fix the first driving component 163 to the light shielding shell. Furthermore, the first transfer block 1635 is L-shaped, and has a simple structure and is convenient to assemble.
In one embodiment, as shown in fig. 1 and 10, the photon counting device 16 further includes a magnet holder 164 for mounting a plurality of magnets 172 of the cleaning device 17.
In an embodiment, as shown in fig. 4 and 6, the bracket 11 is provided with a plurality of first engaging grooves 113 and a plurality of engaging protrusions 112; the first clamping groove 113 and the clamping protrusion 112 are respectively arranged around the upper outer contour and the lower outer contour of the mounting groove 111;
the heating base 131 is provided with a plurality of second card slots 1316 and a plurality of third card slots 1312; the second card slot 1316 is arranged around the upper opening of the cavity 1311, and the third card slot 1312 is arranged around the light outlet 1313;
the first engaging groove 113 is used for engaging with the lower end profile of the light shielding shell 14, and the engaging protrusion 112 is used for engaging with the second engaging groove 1316; the third clamping groove 1312 is used for clamping the outer contour of the light inlet to form a light-shielding sample adding and detecting environment. Specifically, the upper surface and the lower surface of the bracket 11 are provided with a plurality of first clamping grooves 113 and a plurality of clamping protrusions 112, namely, the periphery of the upper contour and the lower contour of each mounting groove 111 are respectively matched with one first clamping groove 113 and one clamping protrusion 112, the heating seat 131 is provided with a plurality of second clamping grooves 1316 so as to be respectively matched with the plurality of clamping protrusions 112, and therefore the airtight assembly of the bracket 11 and the shading shell 14 and the heating seat 131 can be formed. The heating base 131 is provided with a plurality of third locking grooves 1312 for locking the outline of the light inlet of the photon counting device 16, so that a light-shielding light-emitting structure can be realized. By optimizing the structures of the bracket 11 and the heating seat 131, the light-shielding structure of the analysis device 100 can be realized, and the structure is simple, small and small, and occupies a small space.
In one embodiment, the bracket 11 and the reaction cup 12 are replaced simultaneously, so that the operation is simple and convenient, the equipment speed is increased, and the pollution is avoided.
In one embodiment, as shown in FIG. 4, the number of the mounting slots 111 is six, and the carrier 11 is loaded with six cuvettes 12 at a time. It should be understood that the number of mounting slots 111 may also be two, three, four, five, seven or even more to match the configuration of the analytical device 100 for different detection flux requirements.
In one embodiment, as shown in fig. 4, 6 and 8, the mounting groove 111 is provided with a supporting rib 1111 on an inner surface thereof for supporting the third flange 121 of the mouth of the reaction cup 12 to load the reaction cup 12.
In one embodiment, as shown in fig. 4 and 6, the reaction cup 12 has a plurality of contact ribs 122 disposed around the rim for contacting the inner surface of the mounting groove 111. The setting of butt convex rib 122 reduces the contact of reaction cup 12 rim of a cup week side surface and mounting groove 111 to avoid leading to reaction cup 12 assembly insecure because of processing error leads to reaction cup 12 rim of a cup week side surface and mounting groove 111 internal surface mismatch, reduce the requirement to the machining precision.
In one embodiment, as shown in fig. 4 and 6, the bracket 11 extends downward from the periphery to form a sidewall structure 114 for wrapping the outer peripheral surface of the heating seat 131. Specifically, the wrapping structure of the sidewall structure 114 on the outer peripheral surface of the heating base 131 is used to limit the lateral movement of the bracket 11 relative to the heating base 131, and improve the sealing performance of the assembling position of the bracket 11 and the heating base 131, so as to prevent light from entering the reaction cup 12 from the assembling position.
Further, as shown in fig. 4, the sidewall structure 114 is provided with at least two third mounting holes 1141 for being fixed to the sidewall of the heating base 131 by fasteners, so as to increase the mounting firmness between the bracket 11 and the heating base 131.
In one embodiment, as shown in fig. 6, the mounting groove 111 has a through-groove structure. After the reaction cup 12 is mounted in the mounting groove 111, the lower part of the reaction cup 12 is placed outside the bracket 11. After the bracket 11 is installed on the heating base 131, the lower cup body of the reaction cup 12 exposed out of the bracket 11 is directly placed in the cavity 1311 of the heating base 131, and the reaction cup 12 directly contacts the heating environment of the cavity 1311, so that the heating is fast.
In one embodiment, as shown in fig. 4, the second driving assembly 132 includes a ball screw 1321, a slider 1322, a first rail 1323, a base 1324, and a second motor 1325, wherein the first rail 1323 is mounted on the base 1324; the slide block 1322 is connected to the heating base 131; the sliding block 1322 is slidably connected to the first slide rail 1323, and the ball screw 1321 and the sliding block 1322 cooperate to form a transmission structure; one end of the ball screw 1321 is coaxially connected to an output shaft of the second motor 1325, and the other end is rotatably connected to the base 1324. The second motor 1325 rotates to drive the ball screw 1321 to rotate, and the ball screw 1321 rotates to drive the slider 1322 to move linearly along the ball screw 1321, so as to drive the heating seat 131 to move linearly. The first slide rail 1323 is used for guiding and supporting to stabilize the operation of the slide 1322.
In one embodiment, as shown in fig. 4 and 6, the heating base 131 has a fourth recess 1314 for receiving a heating plate. Specifically, the heating plate is attached to the fourth groove 1314, and the arrangement of the heating plate is hidden, so that the appearance is improved, the heating plate is protected, and the heating plate is prevented from being damaged in the assembling process of the heating seat 131. Further, in order to ensure the thermal conductivity of the heating base 131 and reduce the weight of the heating base 131, the heating base 131 is an aluminum alloy member.
Further, the fourth recess 1314 is disposed at the bottom of the heating base 131 for inserting a second adapter block 133 to connect the second driving assembly 132 through the second adapter block 133; the fourth groove 134 and the second transfer block 133 form a space for accommodating the heater chip. Specifically, the fourth recess 1314 is used to accommodate the heating plate, and the second adaptor 133 is inserted into the fourth recess 1314, so that the space of the fourth recess 1314 is effectively utilized, which is beneficial to the miniaturization design of the heating base 131.
In one embodiment, as shown in fig. 6, the light outlet 1313 is convexly contoured to form an extension 1315 for extending into the light inlet of the photon counting device 16. Specifically, the light inlet is formed by the light passage channel 16281 of the light passage nozzle 1628. The outline periphery of the light outlet 1313 realizes a first light blocking structure by clamping the outline of the light passage 16281 and the third clamping groove 1312, and the extension part 1315 extends into the light passage 16281 to form a second light blocking structure. Through two light blocking structures, the light transmitting difficulty of the assembly position of the light outlet 1313 and the light passing channel 16281 is increased, and the light avoiding performance is improved.
In one embodiment, the light shielding shell 14 has a cylindrical structure, and the structure is simple.
In one embodiment, as shown in fig. 7, the bottom of the light shield 181 is provided with a fourth locking groove 1811, and the fourth locking groove 1811 is disposed around the mounting head 182; the top end of the light shielding shell 14 is clamped with the fourth clamping groove 1811 at the bottom of the light shielding cover 181, so that the operation is simple and convenient. In an embodiment, the top profile of the light shielding shell 14 is matched with the fourth engaging groove 1811, and the top profile of the light shielding shell 14 is engaged with the fourth engaging groove 1811 to achieve the engaging. On the one hand, the fourth locking groove 1811 limits the movement of the top end of the light shielding shell 14, and on the other hand, the fourth locking groove 1811 is locked with the top end profile of the light shielding shell 14 to seal the top end opening of the hollow cavity 141, so as to prevent light from entering from the top end opening of the hollow cavity 141.
In an embodiment, the bottom end profile of the light shielding shell 14 is matched with the first slot 113, and the bottom end profile of the light shielding shell 14 is clamped into the first slot 113 to realize clamping. On the one hand, the first engaging groove 113 is used to limit the movement of the bottom end of the light shielding shell 14, and on the other hand, the first engaging groove 113 is used to engage with the bottom end profile of the light shielding shell 14 to seal the bottom opening of the hollow cavity 141, so as to block the light from entering from the bottom opening of the hollow cavity 141 through the bracket 11.
The light shield 181 and the bracket 11 respectively seal the top end and the bottom end of the hollow cavity 141, so as to create a darkroom environment in the hollow cavity 141, and prevent the position of the sample transfer tube 70 exposed outside the reaction cup 12 from being exposed to light to influence the detection accuracy. Further, in the immunoassay, the luminescence reaction is initiated only after the last luminescence excitation substrate is added, in order to simplify the detection operation procedure of the analysis device 100, the light shielding shell 14 is used when the sampling device fills the last luminescence excitation substrate into the reaction cup 12, the detection is started after the last luminescence excitation substrate is filled, and the sampling device is moved away from the sample moving tube 70 after the detection is finished. Other substances such as a sample to be detected, a reagent and magnetic particles are added into the sampling device, and the sample transfer tube 70 is directly inserted into the corresponding reaction cup 12, so that the process of mutually aligning and assembling the light shield 181, the light shield shell 14 and the bracket 11 is omitted.
In an embodiment, as shown in fig. 1, the first driving assembly 163, the second driving assembly 132, the third driving assembly 173, and the fourth driving assembly 1625 are all provided with a position detecting device, such as a light-coupled blocking structure, to detect the movement position information, so as to cooperate with the control module to control the movement path.
Taking the example that the bracket 11 is provided with six mounting grooves 111 for specific explanation, the immunoassay comprises the following specific steps:
s1, the second driving assembly 132 drives the heating base 131 to move to the loading position, and the operator mounts the carrier 11 loaded with a group of six reaction cups 12 on the heating base 131;
s2, the second driving component 132 drives the heating base 131 to move to the sample loading position, and the sampling device 18 sequentially adds the sample to be tested, the reagent, and the magnetic particles into the reaction cup 12;
s3, repeating the step S2, and adding the samples to be detected, the reagents and the magnetic particles into the six reaction cups 12;
s4, the second driving component 132 drives the heating base 131 to reciprocate to oscillate the temperature bath;
s5, after the warm bath is completed, the second driving component 132 drives the heating base 131 to move to the cleaning device 17 for cleaning, the first driving component 163 drives the magnet 172 to align with the corresponding reaction cup 12 to attract the magnetic particles in the reaction cup 12, the third driving component 173 drives the cleaning needle set 171 to extend into the corresponding reaction cup 12, the cleaning needle 1712 is filled with a cleaning solution for cleaning, and the waste liquid is extracted by the waste extraction needle 1711, and the cleaning is completed;
s6, the second driving component 132 drives the heating seat 131 to move to the sample adding position, and the sampling device 18 adds the first substrate; the first driving assembly 163 drives the photon counter 161 and the shutter assembly 162 to move together, so that the light inlet of the shutter assembly 162 is clamped in the third clamping groove 1312 on the periphery of the light outlet 1313 of the heating base 131;
s7, the shading shell 14 is clamped in the first clamping groove 113 corresponding to the reaction cup 12 to be loaded with sample; the sampling device 18 sucks the second substrate, clamps the light shield 181 at the upper end of the light shielding shell 14, rotates the baffle 1624 of the shutter assembly 162 to open the shutter, and conducts the light path for detection;
s8, after the detection is finished, the shutter is closed, and the steps S4 to S7 or the steps S5 to S7 are repeated in sequence to finish the detection of the samples in the rest reaction cups 12.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; those skilled in the art can readily practice the invention as shown and described in the drawings and detailed description herein; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.