CN116539865A - ELISA analyzer and ELISA system - Google Patents

Abstract

The application discloses an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system, which relate to the technical field of biochemical experimental instruments and are used for solving the technical problems that the enzyme-linked immunoassay analyzer in the prior art is too large in size and difficult to carry. The ELISA analyzer comprises an optical detection component. The optical detection component comprises a light guide module and a detection module; the light guide module is configured to change the propagation direction of the detection light, the detection module is arranged at one end of the light guide module, which outputs the detection light, the detection module is provided with a detection vacancy, and the detection vacancy is configured to accommodate the micro-pore plate to be detected; the light guide module and the detection module can move relative to the micro-pore plate to be detected so as to switch the irradiation position of the detection light on the micro-pore plate to be detected. Therefore, the device has the advantages of compact structure and simple and convenient operation.

Description

ELISA analyzer and ELISA system

Technical Field

The application relates to the technical field of biochemical experimental instruments, in particular to an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system.

Background

The microplate detection analyzer (also referred to as microplate reader) is a professional instrument for biochemical experiments commonly used in laboratories, and generally refers to an instrument for detecting specific solution absorbance, fluorescence intensity, luminosity and other detection methods in microplates (6-1536-well plates). An enzyme-linked immunoassay (also called an enzyme-labeled instrument) is a commonly used microplate reader, and the detection principle is enzyme-linked immune reaction. The ELISA reaction is carried out by coupling an enzyme-catalyzed chromogenic substrate on an antigen or an antibody, the reaction result is displayed in color, and the magnitude of an absorbance value can be determined by the color development depth, so that the concentration of the antibody or the antigen to be detected in a sample can be determined.

In order to make the light intensity led into each sample channel as uniform as possible, the existing enzyme-linked immunoassay analyzer generally adopts a light source as a main light source, and achieves the above effects by means of light path beam splitting, light source movement and the like. However, in order to guide light with uniform intensity out to the microplate in a specific direction and to ensure light conduction stability, the conventional enzyme-linked immunoassay analyzer on the market is generally large in size and many in parts to be connected in a matched manner. The existing enzyme-labeled instrument is inconvenient for technicians to carry and carry due to the excessively heavy appearance, has higher requirements on the size of a workbench accommodating space, and is not suitable for being placed in relatively narrow environments such as an incubator or a workstation.

Disclosure of Invention

The application aims to provide an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system, which detect absorbance of each sample in a microwell plate to be detected, which is accommodated in a detection vacancy, through a movable optical detection component. The volume of the ELISA analyzer is effectively reduced, the whole structure is simplified, and the ELISA analyzer has the advantages of being compact in structure and convenient to operate.

Embodiments of the present application are implemented as follows:

An embodiment of the present application provides an enzyme-linked immunoassay analyzer in a first aspect, including an optical detection component. The optical detection component comprises a light guide module and a detection module; the light guide module is configured to change the propagation direction of the detection light, the detection module is arranged at one end of the light guide module, which outputs the detection light, the detection module is provided with a detection vacancy, and the detection vacancy is configured to accommodate the micro-pore plate to be detected; the light guide module and the detection module can move relative to the micro-pore plate to be detected so as to switch the irradiation position of the detection light on the micro-pore plate to be detected.

In the technical scheme, the ELISA analyzer shortens the displacement stroke required by the detection module for detecting the whole plate of the micro-pore plate to be detected by the optical detection component which can move relative to the micro-pore plate to be detected, so that the whole volume of the ELISA analyzer is reduced; in addition, the detection vacancy configured to accommodate the micro-pore plate to be detected, the light guide module and the detection module which are connected with each other, effectively improve the structural compactness of the ELISA; the relative rest of the micro-pore plate to be detected ensures that the sample solution contained in the micro-pore plate can not shake due to movement, thereby effectively improving the accuracy and reliability of the detection result.

In one embodiment, the light guide module includes: the optical fiber bundle and the optical fiber accommodating shell. The optical fiber bundle is provided with a beam combining end and a plurality of branch ends; the beam combining end and each branch end are arranged on the first end face of the optical fiber accommodating shell.

In the above technical scheme, the light guide module is used for fixing the optical fiber bundle and reversing the transmission direction of the detection light by 180 degrees through the beam combining end and the plurality of branch ends which are arranged on the same end face of the optical fiber accommodating shell. The optical fiber can be bent in a short distance to change the light path, and the volume of the light guide module is effectively reduced.

In one embodiment, the height of each branch end relative to the first end surface is uniform.

In the technical scheme, the heights of the branch ends in the light guide module are consistent relative to the first end face, so that polishing of the optical fiber end face during manufacturing and assembling of the light guide module is facilitated, and the consistency of detection light paths corresponding to the branch ends is improved.

In one embodiment, each branch end is connected to the bundle end by a bent optical fiber, and all the optical fibers are solidified in the optical fiber accommodating case.

In the technical scheme, the light guide module improves the stability of light for light transmission detection by solidifying the optical fiber wire and placing the optical fiber wire in the shell; the optical fiber wires do not need to be assembled independently, and are all solidified in the structure in the optical fiber accommodating shell, so that the assembly efficiency of the ELISA analyzer is improved, the optical fibers are effectively prevented from being broken in the transportation and assembly processes of the ELISA analyzer, and the light guide efficiency of the light guide module is higher.

In one embodiment, the detection module includes a sample irradiation portion having a detection void; the end face of one end of the detection vacancy is provided with a plurality of light input holes, and the end face of the other end of the detection vacancy is provided with a plurality of light output holes; the branch end comprises a plurality of sample detection branch ends, one sample detection branch end is accommodated at one end of one light input hole, and the central axis of one light input hole and the central axis of one light output hole coincide.

In the technical scheme, the detection module realizes the irradiation and the derivation of each sample of the micro-pore plate in the detection vacancy after the sample light is derived from the sample detection branch end through the light input holes and the light output holes at the two ends of the detection vacancy. The detection module in the scheme reasonably arranges space, so that the whole structure is more compact, and the independent sample channel detection of a plurality of sample light rays with consistent light intensity is satisfied.

In an embodiment, the detection module further includes a sample detection plate, and the sample detection plate is disposed at one end of the sample irradiation portion and covers all the light output holes.

In the technical scheme, the detection module detects the intensity of the light which passes through the sample solution in the micro-pore plate and is output by the sample detection plate covering all the light output holes.

In an embodiment, the branch end further comprises a reference detection branch end, and the reference detection branch end is arranged at one side of the sample detection branch end; the detection module further includes a reference detection plate coupled to the first end face, the reference detection plate configured to: the light intensity of the detection light output from the reference detection branch end is detected.

In the technical scheme, the detection module detects the light intensity which is actually output and evenly divided by the light source module through the reference detection plate, so that the light intensity fluctuation of the detection light is verified as a reference group, and the accuracy and the reliability of the sample absorbance detection result are improved.

In an embodiment, the detecting module further includes a plano-convex lens, the plano-convex lens is disposed at the other end of the light input hole, and a convex surface of the plano-convex lens faces the detecting vacancy.

In the above technical scheme, the detection module converges the light outputted by the sample detection branch end through the plano-convex lens, so that most of the equally divided detection light irradiates each sample channel in the micro-pore plate.

In an embodiment, the detection module further includes a biconvex lens, and the biconvex lens is disposed at one end of the light output hole near the detection vacancy.

In the above technical scheme, the detection module groups converge the light led out through the sample channels through the biconvex lens, so that each path of converged light irradiates the sample detection plate, and the light intensity detection is carried out on each path of light, so that the absorbance of each sample channel is determined.

In one embodiment, the enzyme-linked immunosorbent assay further comprises: a shell and a transmission part. The micropore plate to be measured is fixed in the shell; the transmission part is arranged in the shell and is configured to pull the detection module or the light guide module to move.

In the above technical scheme, the detection module or the light guide module arranged in the shell is pulled and moved by the transmission component to displace relative to the fixed to-be-detected micro-pore plate and the shell, so that the irradiation position of the detection light on the to-be-detected micro-pore plate is switched to detect the absorbance of the sample solution in other micro-pores on the to-be-detected micro-pore plate.

In one embodiment, the transmission member includes: a synchronous belt and a motor. The synchronous belt is connected with the detection module or the light guide module; the output end of the motor is connected with the synchronous belt.

In the above technical solution, the detection module or the light guide module disposed in the housing moves in the housing by driving the synchronous belt to operate by a motor.

In one embodiment, the transmission member further comprises: at least one guide rail and at least one slider. Wherein the guide rail is connected with the shell; the sliding block is movably arranged on the guide rail and is connected with the light guide module or the detection module.

In the technical scheme, the detection module or the light guide module which is arranged in the shell and connected with the sliding block is matched with the sliding block through the guide rail, so that the support of the optical detection part and the guide during movement are realized, and the stability and the reliability of the optical detection part during movement are improved.

In one embodiment, the housing includes a microplate holder having a microplate receiving recess and at least one clip; one clamping piece is arranged at one end of the accommodating groove of the micro-pore plate, and the micro-pore plate to be tested is detachably arranged in the accommodating groove of the micro-pore plate through the clamping piece.

In the technical scheme, the micro-pore plate to be measured is fixed on the micro-pore plate bracket, and the detachable connection is realized through the cooperation of the clamping piece and the micro-pore plate accommodating groove.

In an embodiment, the optical detection component further includes a light source module, the light source module is disposed at one side of the light guide module, and the detection light output by the light source module irradiates on the light receiving end surface of the light guide module.

In the technical scheme, the ELISA makes reasonable use of the side space of the light guide module, and the light source module is arranged on one side of the light guide module, so that the output detection light can irradiate and cover the light receiving end face of the light guide module. The arrangement of the light source module ensures the light output utilization rate and simultaneously efficiently utilizes the side space of the light guide module, so that the structure of the optical detection component is more compact and reasonable.

In one embodiment, the light source module includes: at least one light emitting element and at least one optical filter. Wherein, a light filter is located the light output of a light emitting component, and the light filter is configured as: light rays with specified wavelengths are screened out as detection light.

In the above technical scheme, the light source module screens out the detection light meeting the detection requirement of the absorbance of the sample solution through the optical filter arranged at the light output end of the light emitting element.

In one embodiment, the light emitting elements are provided in plurality, and the light outputted by each light emitting element is filtered by the optical filter and converged at the light crossing point; the center point of the light receiving end surface coincides with the light crossing point.

In the above technical scheme, the light source module is provided with a plurality of light emitting elements, and the installation angles of the plurality of light emitting elements are reasonably arranged, so that the light rays output by the light emitting elements can be converged at the same light ray intersection point after being screened by the optical filter, and then the light ray receiving end face of the light guide module can receive the detection light with approximately equal intensity output by the light source module without changing the position, thereby improving the stability of absorbance detection and the accuracy of the detection result.

In one embodiment, the light emitting element is a monochromatic diode LED lamp and the filter is a narrowband filter.

In an embodiment, the enzyme-linked immunosorbent assay further comprises a control component, wherein the control component comprises a control module and a communication module, the control module is electrically connected with the detection module and the communication module, and the communication module is configured to: wired and/or wireless connection with the user terminal.

The second aspect of the embodiments of the present application provides an enzyme-linked immunosorbent assay system, which comprises at least one enzyme-linked immunosorbent assay device and a user terminal provided in the first aspect and any embodiment of the present application. Wherein, the user terminal is connected with each ELISA analyzer through a communication module.

In the technical scheme, the control part controls the optical detection part and the communication module through the control module so as to receive the instruction and execute the absorbance detection work of the micro-pore plate to be detected or send the detection data to the user terminal through the communication module, thereby realizing data interaction and data uploading. The ELISA analyzer improves the operation convenience and the automation degree of detection work through the control part, and effectively improves the use experience of users.

Compared with the prior art, the beneficial effects of this application are:

the application provides an enzyme-linked immunoassay analyzer and an enzyme-linked immunoassay system, which detect absorbance of each micropore sample channel of a micropore plate to be detected contained in a detection vacancy through a movable optical detection component and gradually switch detection positions. The optical detection component moves relative to the micropore plate to be detected, so that the displacement size required by the detection module to switch the micropore detection position is effectively shortened, and the whole volume of the ELISA analyzer is reduced; the position of the micro-pore plate to be detected is fixed, so that liquid in a sample channel is static and does not shake, the reliability of an ELISA experiment result is improved due to the reduction of interference factors, and the experiment operation is simpler and more convenient; the arrangement of the detection gaps in the optical detection component enables the whole structure of the ELISA analyzer to be more compact; the light guide module is connected with the detection module, so that the change of a light path and the light conduction are more stable, and the reliability and the accuracy of the detection result of the ELISA are improved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered limiting the scope, and that other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of an ELISA system according to an embodiment of the application;

FIG. 2 is a schematic diagram of an ELISA analyzer according to an embodiment of the disclosure;

FIG. 3 is a schematic diagram of an ELISA analyzer with an upper housing closed according to an embodiment of the present disclosure;

FIG. 4 is a schematic diagram of an ELISA analyzer with an upper housing in an open state according to an embodiment of the disclosure;

FIG. 5 is a schematic diagram of a partial structure of an ELISA analyzer according to an embodiment of the application;

FIG. 6 is a schematic diagram showing the overall structure of an optical detection component according to an embodiment of the present application;

FIG. 7 is a schematic diagram of an overall structure of a light guide module according to an embodiment of the disclosure;

FIG. 8 is a schematic cross-sectional view of a light guide module according to an embodiment of the disclosure;

FIG. 9 is a schematic cross-sectional view of a detection module according to an embodiment of the disclosure;

FIG. 10 is a schematic cross-sectional view of a light source module according to an embodiment of the disclosure;

fig. 11 is a schematic view of an optical path within an optical detection component according to an embodiment of the present application.

Icon: 100-microplates; 1-enzyme linked immunoassay system; 2-a user terminal; 3-enzyme linked immunoassay analyzer; 4-an optical detection component; 4101—a first end face; 4102—a receiving cavity; 410-an optical fiber containment case; 4110-branching end; 4111-sample detection branch end; 4112-reference detection branch end; 411-fiber optic bundle; 4120-a beam combining end; 4121-a light receiving end face; 4130-fiber optic; 41-a light guide module; 420-detecting a vacancy; 421-mounting void; 422-sample illumination section; 423-a light output aperture; 424-light input hole; 425-a connector; 42-a detection module; 430-a light source mounting bracket; 431-a light emitting element; 432-an optical filter; 433-ray intersection; 43-a light source module; 441-a photosensor; 44-sample assay plate; 45-a reference assay plate; 461-lens press ring; 46-a biconvex lens; 47-plano-convex lens; a 5-housing assembly; 51-a bottom plate; 520-microplate accommodating bin; 521-clamping pieces; 522-silk screen; 52-microplate holders; 53-an upper housing; 54-a first side plate; 550-guiding groove; 551-switch slide; 552-a stopper; 553 a housing sensor; 554-magnets; 55-a second side panel; 6-a transmission part; 61-an electric motor; 62-synchronous belt; 63-a guide rail; 64-slide block; 71-a control module; 721-a first data interface; 722-a power interface; 723-a second data interface; 72-a communication module; 73-a Bluetooth module; 74-a switch button; 7-control means.

Detailed Description

The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc. are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use for the product of the application, are merely for convenience of description and simplification of the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and therefore should not be construed as limiting the present application.

In the description of the present application, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.

The technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings.

Referring to fig. 1, fig. 1 is a schematic structural diagram of an enzyme-linked immunoassay system 1 according to an embodiment of the present disclosure. The enzyme-linked immunoassay system 1 comprises at least one enzyme-linked immunoassay instrument 3 and a user terminal 2. The enzyme-linked immunoassay analyzer 3 may be provided with one (as shown in fig. 1) or a plurality of enzyme-linked immunoassay analyzers based on actual detection requirements. Each enzyme-linked immunoassay analyzer 3 establishes communication connection with the user terminal 2 through the communication module 72, and the enzyme-linked immunoassay analyzer 3 sends the detected experimental data to the user terminal 2 through the communication module 72 after detection is completed, so that technicians can check the experimental data. In addition, the technician can also control the work of the enzyme-linked immunoassay analyzer 3 through the user terminal 2, including controlling the enzyme-linked immunoassay analyzer 3 to be opened, suspended, ended, opened or closed, and the like.

The user terminals 2 include wired terminals and wireless terminals. The user terminal 2 may be a mobile phone, a tablet computer or a notebook computer provided with control software to control the operation of the enzyme-linked immunosorbent assay device 3. The wireless terminal is connected with the communication module 72 of the ELISA analyzer 3 through a wireless communication module so as to transmit and interact data; the wired terminal is connected with the ELISA analyzer 3 by a data line connection mode so as to transmit and exchange data. Different types of user terminals 2 may meet different application scenarios or different usage requirements. The resulting detection report may also be transmitted or backed up by the technician to a laboratory terminal or laboratory server via the user terminal 2 networking.

Referring to fig. 2, fig. 2 is a schematic structural diagram of an enzyme-linked immunoassay analyzer 3 according to an embodiment of the present disclosure. As shown in fig. 2, the present application provides an enzyme-linked immunoassay analyzer 3, which includes a housing, an optical detection member 4, a transmission member 6, and a control member 7. Wherein, the micro-porous plate 100 to be measured is fixed in the casing, the optical detection component 4 includes a light source module 43, a light guide module 41 and a detection module 42, the detection module 42 is provided with a detection vacancy 420 for accommodating the micro-porous plate 100 to be measured, the detection module 42 is arranged at one end of the light guide module 41 for outputting detection light, and the light source module 43 is arranged at one side of the detection module 42.

The light guide module 41 is configured to change the propagation direction of the detection light, wherein the light guide module 41 and the detection module 42 can move relative to the microplate 100 to be detected to switch the irradiation position of the detection light on the microplate 100 to be detected. The transmission member 6 is disposed in the housing and the transmission member 6 is configured to move the traction detection module 42 or the light guide module 41. The control part 7 is arranged on the shell and is electrically connected with the detection module 42, the light source module 43 and the transmission part 6 to control the enzyme linked immunosorbent assay instrument 3 to work or stop working, and generates experimental data based on the data detected by the detection module 42, and exchanges information with the user terminal 2, etc.

In an operation process, an operator presets operation parameters of the enzyme-linked immunoassay analyzer 3 on the user terminal 2 through relevant control software of the enzyme-linked immunoassay analyzer 3. Then, the top case (i.e., the upper case 53) of the enzyme-linked immunoassay analyzer 3 is opened, and after the operator mounts and fixes the microwell plate 100 to be measured to a designated position, the top case (i.e., the upper case 53) is closed. The operator sends a detection start instruction through the user terminal 2, and after receiving the detection start instruction, the control part 7 controls the light source module 43 and the detection module 42 to be started, and simultaneously controls the transmission part 6 to act so as to drive the detection module 42 or the light guide module 41 connected with the transmission part to move relatively with the micro-pore plate 100 to be detected until the detection light can irradiate to the micro-pore sample channel.

The control module 71 controls the light source module 43 to light and output detection light with a specified wavelength based on detection configuration information carried by a detection start instruction, the light guide module 41 divides the detection light into multiple paths of uniform light with consistent light intensity and irradiates the light into part of the micro-pore sample channels of the micro-pore plate 100 to be detected, and the detection module 42 detects the light intensity finally output after the light passes through the sample so as to determine the absorbance of the sample in each micro-pore sample channel.

After the absorbance detection of one group of sample channels is completed, the control part 7 drives the light guide module 41 or the detection module 42 to move relative to the to-be-detected micro-pore plate 100 again so as to switch the irradiation position of the detection light to other micro-pore sample channels of the to-be-detected micro-pore plate 100, and then the enzyme-linked immunoassay analyzer 3 detects the absorbance of samples at other positions again through the detection module 42. The enzyme linked immunoassay analyzer 3 is repeatedly executed according to the steps until all the micropore sample channels on the micropore plate 100 to be detected are detected, namely, the full plate detection of the micropore plate 100 to be detected is completed.

After the detection is completed, the transmission part 6 drives the optical detection part 4 to move to the original point, and the enzyme-linked immunoassay analyzer 3 generates an experiment report or an experiment log based on the detection data and sends the experiment report or the experiment log to the user terminal 2. Then, the upper case 53 of the enzyme-linked immunoassay analyzer 3 is opened again, and the operator takes out the detected microplate 100 from the case, and then the upper case 53 is closed again, so that the detection of one microplate 100 by the enzyme-linked immunoassay analyzer 3 is completed.

Referring to fig. 3 to 5, fig. 3 is a schematic structural diagram of an enzyme-linked immunoassay analyzer 3 in a closed state of an upper housing 53 according to an embodiment of the present disclosure; fig. 4 is a schematic structural diagram of an enzyme-linked immunoassay analyzer 3 in an opened state of an upper housing 53 according to an embodiment of the present disclosure; fig. 5 is a schematic diagram showing a partial structure of an enzyme-linked immunoassay analyzer 3 according to an embodiment of the present application. Referring to fig. 2 to 5, the housing includes a bottom plate 51, an upper housing 53, a first side plate 54, a second side plate 55 and a microplate holder 52. Wherein, the first side plates 54 are two and are arranged at two opposite ends of the bottom plate 51 along the first straight line direction; the second side plates 55 are provided at two opposite ends of the bottom plate 51 in the second linear direction. The first linear direction is the moving direction of the optical detection component 4 relative to the microwell plate 100 to be detected, and the first linear direction is perpendicular to the second linear direction and is horizontal.

The upper housing 53 is slidably provided on top of the second side plate 55. When the upper housing 53 completely covers the top of the bottom plate 51, the upper housing 53, the first side plate 54, the second side plate 55 and the bottom plate 51 form a detection space for accommodating the optical detection component 4, the transmission component 6 and the micro-porous plate 100 to be detected, and at this time, the upper housing 53 is in a closed state, and the two first side plates 54 just close the openings at two ends of the upper housing 53. The top end of the second side plate 55 is provided with a guide groove 550 for accommodating the switch slide block 551 and allowing the switch slide block 551 to move, and one ends of two side walls of the upper shell 53 are respectively connected with the second side plate 55 through the switch slide block 551. The upper housing 53 is movably disposed on the second side plate 55 by the engagement of the switch slider 551 with the guide groove 550 to achieve an opened state and a closed state of the upper housing 53.

One end of the guide groove 550 of the second housing is provided with a limiting member 552 for limiting the displacement travel of the switch slider 551, so as to prevent the upper housing 53 from sliding off the second side plate 55 in the opened state. A housing sensor 553 is further provided at the edge of the first side plate 54 or the edge of the second side plate 55 near the side of the stopper 552. The upper shell 53 is in a closed state, a baffle arranged in the upper shell 53 triggers the shell sensor 553, the control part 7 receives a trigger signal of the shell sensor 553, and the ELISA analyzer 3 is determined to be in a closed state and can continue to detect; when the upper housing 53 is in an unclosed state (the upper housing 53 is not tightly closed or opened), the baffle arranged in the upper housing 53 does not trigger the housing sensor 553, the control part 7 does not receive the trigger signal of the housing sensor 553, and cannot respond to the detection start instruction to detect, at this time, the enzyme-linked immunoassay analyzer 3 can send a prompt message to the user terminal 2 through the control part 7 to prompt the user that the upper housing 53 is not closed.

In one embodiment, the upper housing 53 is made of iron, and the first side plate 54 near the side of the limiting member 552 is further provided with at least one magnet 554. When the upper housing 53 moves and is about to be covered, the magnet 554 of the first side plate 54 attracts the back plate on one side of the upper housing 53, thereby assisting the upper housing 53 to complete the covering action and close the door Yan Fengxi.

In one embodiment, the microplate holder 52 is fixed on the bottom plate 51, the microplate holder 52 has a microplate accommodating recess 520 and at least one clamping member 521, and the clamping member 521 is configured to: limiting and fixing the to-be-measured micro-pore plate 100 in the micro-pore plate accommodating groove 520 along the linear direction. In an embodiment, one clamping member 521 is disposed at one end of the microplate accommodating groove 520 along the first linear direction, and one clamping member 521 is disposed at one end of the microplate accommodating groove 520 along the second linear direction. The microplate 100 to be measured is detachably disposed in the microplate accommodating recess 520 by the fastening member 521. The clamping member 521 may be a spring plate, a spring, etc.

The clamping member 521 is fixed to the edge of the microplate accommodating recess 520 by means of screws or glue. The microplate 100 to be measured is abutted between the microplate accommodating groove 520 of the microplate bracket 52 and the clamping piece 521 under the opposite acting force of the clamping piece 521, so that the limiting and fixing of the microplate bracket 52 to be measured on the microplate 100 are realized.

In an operation process, a technician places the microplate 100 to be measured in the microplate accommodating groove 520 of the microplate support 52 according to the direction indicated by the silk screen 522 on the microplate support 52 (i.e. the mark on the microplate 100 is aligned with the silk screen 522), and the microplate 100 to be measured can be firmly fixed on the microplate support 52 under the reaction force of the clamping member 521. After the microplate 100 to be measured is placed, the upper housing 53 moves along the guide groove 550 through the switch slider 551 toward the first side plate 54 provided with the switch button 74, and because the upper housing 53 is machined by using an iron plate, when the upper housing 53 moves to the position to be closed, the magnet 554 on the first side plate 54 at the other end of the bottom plate 51 will attract the back plate of the upper housing 53 under the action of magnetic force, so as to complete the closing action.

The shell sensor 553 is shielded by the baffle to generate a corresponding trigger signal, and the enzyme-linked immunoassay analyzer 3 can continuously detect after receiving the trigger signal; if the housing sensor 553 is not blocked by the blocking sheet and does not generate a corresponding trigger signal, the enzyme-linked immunosorbent assay analyzer 3, in the uncapped state, if receiving a detection start instruction sent by the user terminal 2, will alarm or send a prompt message to the user terminal 2 to prompt the user: the instrument is not closed, the next detection can not be carried out, the user is requested to check the closing of the cover, and the normal detection can be carried out after the closing of the cover. The shell component 5 can effectively avoid damage to the detection module 42 caused by incomplete closing of the upper shell 53 and exposure of the detector, improve the operation reliability of the ELISA analyzer 3, prolong the service life and improve the use experience of a user.

Referring to fig. 6, fig. 6 is a schematic diagram illustrating an overall structure of the optical detection component 4 according to an embodiment of the present application. As shown in fig. 6, the optical detection component 4 includes a light source module 43, a light guide module 41, and a detection module 42. Wherein, a light source module 43 is disposed at one side of the detection module 42, and the light guide module 41 is disposed at one end of the detection module 42 and one end of the light source module 43. The detection module 42 includes a sample irradiation portion 422 and a sample detection plate 44, the sample detection plate 44 being disposed at the other end of the sample irradiation portion 422 (the sample detection plate 44 and the light guide module 41 being disposed at the opposite ends of the sample irradiation portion 422). A detection void 420 is provided in the center of the sample irradiation portion 422, and the detection void 420 is configured to accommodate the microplate 100 to be measured. One end of the detection space 420 of the sample irradiation part 422 is provided with a plurality of light input holes 424 for the detection light to irradiate the sample channel, and the other end of the detection space 420 of the sample irradiation part 422 is provided with a plurality of light output holes 423 for absorbing light through the sample, outputting the light and conducting the light to the sample detection plate 44.

When the optical detection unit 4 is operated, the control unit 7 controls the light source module 43 to output detection light of a predetermined wavelength. The detection light is conducted to the light guide module 41, the light path propagation direction is changed through the light guide module 41, the light intensity is equally divided, the detection light is output to the sample irradiation part 422, the multipath detection light is output through the light guide module 41 and is irradiated to a plurality of sample channels of the micro-pore plate 100 to be detected through the light input hole 424, after the detection light is absorbed by the sample to be detected in the sample channels, the residual light is irradiated to the sample detection plate 44 through the sample channels and the light output hole 423, a plurality of photoelectric sensors 441 for detecting the light intensity are arranged on one surface of the sample detection plate 44 close to the light output hole 423, the photoelectric sensors 441 detect the light intensity of the light output through the light output hole 423 and convert the light intensity into corresponding electric signals, and the control part 7 receives the electric signals and calculates the absorbance corresponding to each sample channel.

Referring to fig. 7 to fig. 8, fig. 7 is a schematic diagram illustrating an overall structure of a light guide module 41 according to an embodiment of the disclosure; fig. 8 is a schematic cross-sectional view of a light guide module 41 according to an embodiment of the disclosure. Referring to fig. 6 to 8, the light guide module 41 includes a plurality of optical fiber bundles 411 and an optical fiber accommodating case 410 for accommodating the optical fiber bundles 411. Both ends of the optical fiber bundle 411 are disposed at a first end surface 4101 of the optical fiber accommodating case 410, and a middle portion of the optical fiber bundle 411 is cured in the accommodating cavity 4102 of the optical fiber accommodating case 410.

The optical fiber bundle 411 has a bundle end 4120 and a plurality of branch ends 4110, wherein the bundle end 4120 and each branch end 4110 are disposed on the first end surface 4101 of the optical fiber housing shell 410, and the heights of each branch end 4110 relative to the first end surface 4101 are consistent. Each branch end 4110 is connected to the bundle end 4120 by a bent fiber wire 4130, and all the fiber wires 4130 are cured in the fiber accommodating case 410 by a glue filling process. The branch end 4110 includes a plurality of sample detecting branch ends 4111 and a reference detecting branch end 4112, wherein the reference detecting branch end 4112 is disposed at one side of the sample detecting branch end 4111. In one embodiment, each of the branch ends 4110 and the combiner end 4120 is disposed on the top surface of the optical fiber accommodating housing 410.

In one embodiment, all the sample detecting branches 4111 may be uniformly arranged in one, two or more rows along the first linear direction to detect the corresponding rows of micro-porous sample channels on the micro-porous plate 100. The arrangement of the sample detecting branch ends 4111 is specifically determined according to the arrangement of the plurality of micro holes on the micro hole plate 100 to be detected.

In one embodiment, the fiber bundle 411 is of a nine-segment design, and the sum of the fiber bending angles is 180 ° (180 ° reversal of the propagation direction of the detection light is achieved). The lengths of the 9 optical fiber filaments 4130 are each inconsistent depending on the bending curvature of the optical fiber filaments 4130 and the position of each branch end 4110 relative to the combiner end 4120. Wherein, the size of the 9-in-1 quartz optical fiber can be controlled within the range of 12.5 mm.

The optical fiber 4130 of the light guide module 41 provided in the application does not use the traditional sheathing manner, and directly uses the optical fiber accommodating case 410 as a protective case for the optical fiber 4130, and the optical fiber 4130 of the optical fiber bundle 411 is placed in the accommodating cavity 4102 of the optical fiber accommodating case 410, so that the external dimension of the optical fiber can be effectively reduced, and the bending curvature radius of the optical fiber and the volume of the light guide module 41 can be further reduced. The integrated design of the light guide module 41 can further reduce the volume of the ELISA 3, has compact space and improves the consistency of detection light in each sample channel. The optical fiber bundle 411 is used as a modularized part, and can be directly solidified into the optical fiber accommodating shell 410 according to the positioning requirement and the light path bending direction in the assembly process, so that the optical fiber 4130 of each branch is not required to be assembled independently, and the assembly efficiency of the enzyme-linked immunosorbent assay instrument 3 is improved.

The light guide module 41 fixes the optical fiber 4130 in the accommodating cavity 4102 of the optical fiber accommodating case 410 by adopting a glue filling process, so that the light guide module 41 becomes a solidified module, and the specific method is as follows: black silica gel is injected into the accommodating cavity 4102 where the optical fibers are fixed, so as to cure each optical fiber 4130 and improve the stability of the optical fibers. The black silica gel can reduce the optical path crosstalk caused by the broken fiber of the optical fiber bundle 411. The optical fiber 4130 is solidified by adopting liquid black silica gel, so that the optical fiber bundle 411 and the light guide module 41 can be effectively prevented from being broken in the transportation and assembly processes of the enzyme-linked immunoassay analyzer 3, and the stability of the optical fiber is improved. In addition, the light guide module 41, in which the optical fiber 4130 is bent to change the direction of light transmission for detection and the optical fiber 4130 is cured by the potting process, has higher light guide efficiency than the light guide column.

The light guide module 41 provided by the application can effectively reduce the length and the volume of the optical fiber, so that the structural space of the instrument is more compact, the structure is small, the protection level of the optical fiber is improved, and the protection performance of the optical fiber is obviously enhanced compared with that of the traditional optical fiber.

The plurality of branch ends 4110 and the beam combining end 4120 of the optical fiber bundle 411 are fixed on the top surface of the optical fiber accommodating housing 410 in the form of metal heads, and the exposed end surfaces of the plurality of branch ends 4110 and the beam combining end 4120 are the same in height (h) relative to the top surface of the optical fiber accommodating housing 410. The exposed end surfaces with the same height are convenient for polishing the end surfaces of the optical fibers during the manufacturing and assembly of the ELISA 3, and the consistency of detection light paths corresponding to the branch ends 4110 is improved.

Referring to fig. 9, fig. 9 is a schematic cross-sectional view of a detection module 42 according to an embodiment of the disclosure. Referring to fig. 6 and 9, the detecting module 42 includes a sample irradiating portion 422, a sample detecting plate 44, a reference detecting plate 45, a biconvex lens 46, a plano-convex lens 47, and a lens pressing ring 461.

In one embodiment, the sample illumination portion 422 has a detection void 420; the end face of one end of the detection vacancy 420 is provided with a plurality of light input holes 424, and the end face of the other end of the detection vacancy 420 is provided with a plurality of light output holes 423; one sample detection branch end 4111 is accommodated at one end of one light input hole 424, and the central axis of one light input hole 424 coincides with the central axis of one light output hole 423. The sample detection plate 44 is provided at one end of the sample irradiation portion 422 and covers all the light output holes 423, the reference detection plate 45 is connected to the first end surface 4101 of the optical fiber accommodating case 410, and the reference detection plate 45 is configured to: the light intensity of the detection light output from the reference detection branch terminal 4112 is detected.

In one embodiment, the plano-convex lens 47 is disposed at the other end of the light input hole 424, and the convex surface of the plano-convex lens 47 faces the detection space 420. The lenticular lens 46 is disposed at one end of the light output aperture 423 near the detection void 420. A plurality of photosensors 441 are provided on an end face of the sample detection plate 44 adjacent to the light output hole 423, and one photosensor 441 is positioned corresponding to one sample output hole, the photosensors 441 being configured to detect the light intensity output by the light output hole 423.

In one embodiment, each light input hole 424 is disposed at the bottom end of the detection space 420, and each light output hole 423 is disposed at the top end of the detection space 420 and is located directly above each light input hole 424. The sample detection plate is fixed to the sample irradiation section 422 by means of a screw or the like. The sample detection plate is provided with 8 photoelectric sensors 441 to respectively test the light intensity outputted from the light output hole 423 after light absorption through each sample channel in the microplate 100 to be detected, and then used for absorbance calculation.

The 8 photoelectric sensors 441 on the sample detection board 44 respectively correspond to the 8 sample detection branch ends 4111 of the light guide module 41, the detection light received by the combination Shu Duan 4120 of the optical fiber bundles 411 in the light guide module 41 is conducted by the optical fiber 4130 and changed in the light path direction, and then is uniformly divided into 9 light rays with consistent and stable light intensity, 8 sample light rays are output from the sample detection branch end 4111, and 1 reference light ray is output from the reference detection branch end 4112. The light outputted from the sample detecting branch end 4111 is focused by the plano-convex lens 47 in the light input hole 424, then irradiates onto a plurality of micro-pore sample channels on the micro-pore plate 100 to be detected, which are accommodated in the detecting vacancy 420, is focused again by the biconvex lens 46 in the light output hole 423 after being absorbed by the sample solution, and is outputted onto the photoelectric sensor 441 to be converted into an electric signal for processing and analyzing the absorbance of the sample solution in each micro-pore.

The reference detection plate 45 is arranged at the top of the reference detection branch end 4112 of the light guide module 41, the reference light output by the reference detection branch end 4112 directly irradiates on the reference detection plate 45 through a small hole (the reference detection plate 45 is also provided with a photoelectric sensor 441), and is converted into an electric signal through the photoelectric sensor 441 on the reference detection plate 45 for processing and analyzing the light intensity fluctuation of the reference light. The reference detection branch end 4112 and the reference detection plate 45 are used for adjusting the absorbance difference caused by the unstable light source.

During the detection process, the intensity of the light emitted by the light source may be attenuated or increased with the detection of each line of samples, and thus the absorbance calculation result may be higher or lower. The light intensity detection of the reference control group can effectively reduce the absorbance calculation deviation. Since the reference light is directly irradiated onto the photosensor 441 of the reference detection plate 45, when the light of the light source fluctuates, the intensity of the reference light detected by the reference detection plate 45 also changes. Therefore, the configuration of the reference detecting branch end 4112 and the reference detecting board 45 can effectively improve the accuracy and stability of the test result of the enzyme-linked immunoassay analyzer 3, and the testing process is more reliable.

Referring to fig. 10, fig. 10 is a schematic cross-sectional view of a light source module 43 according to an embodiment of the disclosure. As shown in fig. 10, the light source module 43 includes a light source mounting bracket 430, at least one light emitting element 431 and at least one filter 432. The optical filter 432 is disposed at the light output end of the light emitting element 431, and the optical filter 432 and the light emitting element 431 are disposed in the light source mounting bracket 430. The filter 432 is configured to: light rays with specified wavelengths are screened out as detection light. The light emitting elements 431 may be provided in plurality, and the installation angles of the light emitting elements 431 and the corresponding optical filters 432 are consistent, so that the included angles formed by the light output by each optical filter 432 and the light receiving end face 4121 are equal, and are all alpha. The light beams output by the light emitting elements 431 are filtered by the optical filters 432 and are collected at the light intersecting points 433, and the center point of the light receiving end face 4121 (i.e., the end face of the beam combining end 4120 of the optical fiber bundle 411 in the light guiding module 41) coincides with the light intersecting points 433.

In an embodiment, a mounting space 421 is further provided on one side of the sample irradiation portion 422, and the mounting space 421 is configured to mount the light source module 43 and provide a light transmission space. The light source module 43 is disposed in the mounting space 421 on the side of the sample irradiation portion 422 and is located at one end of the mounting space 421, and the splice Shu Duan 4120 of the optical fiber bundle 411 in the light guide module 41 is disposed in the mounting space 421 on the side of the sample irradiation portion 422 and is located at the other end of the mounting space 421. The detection light output from the light source module 43 irradiates the light receiving end face 4121 of the light guide module 41, i.e., the end face of the beam combining end 4120 of the optical fiber bundle 411.

In an embodiment, two light emitting elements 431 and filters 432 are respectively disposed, the light emitting elements 431 are monochromatic diode LED lamps, and the filters 432 are narrow-band filters 432. Each light source module 43 can realize the light output of detection of two wavelengths, and two light emitting elements 431 form a certain included angle. When the control part 7 controls the different light emitting elements 431 to be turned on, the light receiving end face 4121 of the light guiding module 41 can receive the corresponding detection light, and transmit the detection light to the micro-porous plate 100 to be detected through the sample detection branch end 4111, and irradiate the sample detection plate 44 through the micro-porous plate 100 to be detected.

The light source module 43 is fixed at one end of the installation space 421 through a magnet 554, and the control part 7 supplies power and controls the monochromatic diode LED lamp in the light source module 43 through the quick-plug connector to obtain monochromatic light with required wavelength. The application provides a light source module 43 can be quick plug change, simple to operate is swift. When a new light source module 43 needs to be replaced, the upper housing 53 of the enzyme-linked immunosorbent assay device 3 is switched to an open state, and the detection module 42 moves along the first linear direction to expose the light source module 43. The technician pulls out the old light source module 43, inserts the new light source module 43 again, updates the information of the light source module 43 on the user terminal 2, and performs the related test to complete the replacement of the light source module 43. The light source module 43 provided by the application has the advantages of small volume, low power, small heating value, long service life and low cost compared with the form of the conventional halogen lamp or xenon lamp matched with the light filtering device.

Referring to fig. 11, fig. 11 is a schematic view of an optical path in the optical detection component 4 according to an embodiment of the present disclosure. As shown in fig. 11, in an operation process, the enzyme-linked immunosorbent assay 3 transmits light during detection: the light-emitting element 431 of the light source module 43 emits light, and the light passes through the corresponding filter 432 to filter the emitted light once, so as to obtain monochromatic light (i.e. light for detection) meeting the test requirement. The beam of detection light irradiates the incident end face (i.e., the light receiving end face 4121) of the light guide module 41 at a certain angle, and the light spot of the detection light is large enough to cover all the optical fibers 4130 on the incident end face of the light guide module 41.

The light guide module 41 equally divides the light and changes the transmission direction through the optical fiber bundle 411, and then transmits the light to each branch end 4110, so as to complete the light emission. The light of the detection light completes 180-degree direction change and 1-9 transmission in a narrow space. Each sample detecting branch end 4111 transmits the sample light upward to the plano-convex lens 47, the sample light upward penetrates through the substance to be detected in the micro-porous plate 100 through the plano-convex lens 47, and then the light is converged on the photoelectric sensor 441 through the above double-convex lens 46, so as to complete the detection of the light intensity. The reference light is emitted from the reference detection branch end 4112, and the reference light is directly irradiated onto the photoelectric sensor 441 of the reference detection plate 45, so as to monitor the fluctuation of the intensity of the detection light, and improve the accuracy of the absorbance test result.

During the detection process, the control unit 7 of the enzyme-linked immunosorbent assay 3 controls the corresponding light emitting element 431 to emit light according to the preset wavelength of the detection light. At each detection, only one light emitting element 431 is lighted to emit light; in response to the need for a dual wavelength detection light test, the enzyme-linked immunoassay device 3 performs detection light detection at the second wavelength after detection light detection at the first wavelength is completed.

The light source module 43 that this application provided locates the top one side of sample irradiation portion 422, and the light that two light emitting components 431 sent becomes certain contained angle, and all can shine the incident end face of fiber bundle 411, and light source module 43 make full use of detection module 42's side space has effectively reduced the light source and has assembled the required space that occupies, has improved the holistic compact of instrument. The detection light outputted from the light source module 43 is subjected to 180 DEG inversion and uniform derivation of 1:9 by the modularized light guide module 41, thereby achieving the purposes of folding and scattered transmission of the detection light in space.

In the optical detection component 4, the light guide module 41 and the detection module 42 are fixed together, the light source module 43 is also arranged on one side of the detection module 42 and moves along with the detection module, the light source, the optical fiber and the photoelectric sensor 441 do not have relative displacement in the whole detection process, the relative positions of all optical paths equally divided by the optical fiber do not deviate, the light intensity transmission efficiency basically does not change, and the stability of the optical paths can be ensured.

The enzyme-linked immunoassay appearance 3 that this application provided space utilization is high, under the condition that satisfies detection light ray conduction and conveniently changes light source module 43, can the at utmost compress and utilize the interior space size of analysis appearance, accomplishes the transmission of light and the change of light conduction direction in narrow and small space. The whole volume of the ELISA 3 is effectively reduced, parts are more compact, and the assembly and manufacturing cost of the ELISA 3 are relatively low.

Referring to fig. 2 to 5, the transmission member 6 is configured to pull the optical detection member 4 to move, and the transmission member 6 includes a timing belt 62 and a motor 61. The motor 61 is disposed on the bottom plate 51 of the housing, one end of the synchronous belt 62 is connected with an output shaft of the motor 61, and after the motor 61 is turned on, the synchronous belt 62 is driven by the motor 61 to start to operate, and drives the detection module 42 or the light guide module 41 connected with the synchronous belt 62 to move. In one embodiment, a connector 425 configured to connect with the timing belt 62 is disposed at one end of the light irradiation portion of the detection module 42.

In other embodiments of the present application, the driving member 6 may be driven by a motor 61 and a screw rod and a nut are matched to drive the optical detection member 4 to move. The screw rod is connected with an output shaft of the motor 61, the output shaft drives the screw rod to rotate, the nut converts rotary motion into linear motion through cooperation with the screw rod, displacement occurs on the screw rod along the linear direction, and the light guide module 41 or the detection module 42 connected with the nut moves along the linear direction along with the nut, so that the optical detection component 4 moves relative to the micro-pore plate 100 to be detected.

In one embodiment, the transmission member 6 further comprises at least one guide rail 63 and at least one slider 64. The guide rail 63 is disposed on the bottom plate 51 of the housing, the slider 64 is movably disposed on the guide rail 63, and the slider 64 is connected to the bottom end of the optical fiber accommodating case 410 of the light guiding module 41. The optical fiber accommodating case 410 of the light guide module 41 plays a role of switching support. As shown in fig. 2, the guide rail 63 is provided in two and parallel to the linear movement direction of the timing belt 62. The support of the double guide rail 63 and the plurality of sliding blocks 64 can ensure that the detection module 42 slides smoothly and moves stably when the ELISA 3 detects. The synchronous belt 62 drives the optical detection component 4 to move along the first linear direction so as to realize detection of the whole plate sample of the microplate 100 to be detected, and the microplate 100 to be detected is stationary relative to the bottom plate 51 of the housing. When the ELISA analyzer 3 detects a sample in the microplate 100 to be detected, the sample liquid is static and has no shaking, the test efficiency is higher, the result is more accurate, and the repeatability is better.

In the motion detection mode that the to-be-detected micro-pore plate 100 is fixed and the optical detection component 4 moves relative to the to-be-detected micro-pore plate 100, the corresponding displacement stroke of the optical detection component 4 is approximately equal to the length of one to-be-detected micro-pore plate 100, the overall size of the ELISA analyzer 3 can be effectively reduced, the structure of the instrument is compact, and the total length of the instrument is smaller than the sum of the lengths of the two to-be-detected micro-pore plates 100.

Referring to fig. 2 to 5, the control unit 7 includes a control module 71 and a communication module 72, the control module 71 is electrically connected with the detection module 42 and the communication module 72, and the communication module 72 is configured to: wired and/or wireless with the user terminal 2. The communication module 72 includes: a bluetooth module 73, a first data interface 721, a second data interface 723. The first datase:Sub>A interface 721 may be ase:Sub>A USB-ase:Sub>A type datase:Sub>A interface, and the second datase:Sub>A interface 723 may be ase:Sub>A USB-B type datase:Sub>A interface.

The control module 71 and the communication module 72 may be integrated on an electronic control motherboard, where the control module 71 includes a core controller and a control driver, and the electronic control motherboard is fixed on the bottom board 51. In one embodiment, the control unit 7 further includes a switch button 74 and a power supply interface 722, and the power supply interface 722 is Type-C. The switch button 74 and the power supply interface 722 are electrically connected to the control module 71.

The user terminal 2 can be divided into a wired terminal and a wireless terminal, wherein the wireless terminal is a mobile phone or a tablet computer with instrument control software, and communication connection is established between the wireless terminal and a Bluetooth module 73 in the control part 7 through the Bluetooth module; the wired terminal such as a PC computer can control the ELISA analyzer 3 through instrument control software and a mode that a data wire is connected with a data interface. Different user terminals 2 can meet the use requirements in different scenes or the use habits of different technicians. The user terminal 2 can transmit or back up the obtained test data or test report to a server of a corresponding company or laboratory system through networking, so that the data can be conveniently stored and interacted.

If the ELISA 3 is in a relatively small space, such as an incubator or a workstation requiring a closed environment, a technician may use a wireless connection. The user terminal 2 and the ELISA analyzer 3 are connected through Bluetooth to set and modify the operation parameters of the instrument and send the execution instructions related to the test. After the detection is completed, the enzyme-linked immunoassay analyzer 3 returns the test result to the user terminal 2 connected wirelessly. After the user terminal 2 sends the execution command, the enzyme-linked immunoassay analyzer 3 detects and temporarily backs up the detection data in its own memory, the user terminal 2 can be far away from the enzyme-linked immunoassay analyzer 3 or is disconnected with the instrument at this time, and the user terminal 2 re-returns to the Bluetooth transmission range of the instrument and re-establishes connection with the Bluetooth of the instrument. After bluetooth pairing, the enzyme linked immunosorbent assay 3 will transmit the measured data to the user terminal 2 without interruption.

The optical detection component 4 of the enzyme-linked immunoassay analyzer 3 provided by the application comprises a light source module 43, a light guide module 41 and a detection module 42 which are assembled into a whole, and the detection module is displaced relative to synchronous movement of the microplate 100 to be detected so as to switch the detection position. In the whole detection process, no relative motion exists among the light source module 43, the optical fiber 4130 and the photoelectric sensor 441, and the light intensity transmission efficiency of the detection light cannot be changed. Therefore, the whole ELISA analyzer 3 has the advantages of small volume, compact light path structure, relatively low cost and convenient operation. In the absorbance detection process, the sample liquid in the fixed microplate 100 is relatively static, so that the test result is more accurate and reliable, and the stability of the detection process is high.

The ELISA analyzer 3 provided by the application supports wireless and wired control, and can meet the use requirements of different user terminals 2 in different scenes. The ELISA analyzer 3 does not need to additionally set interactive hardware such as a display screen, a key module and the like, so that the volume of the ELISA analyzer 3 is effectively reduced, the whole structure of the ELISA analyzer 3 is more compact, a user can realize checking of a detection report and configuration of detection parameters through a user terminal 2 provided with instrument control software, and the assembly cost and the manufacturing cost of the ELISA analyzer 3 are effectively reduced on the premise that the basic function is unchanged. The user terminal 2 may transmit or back up the acquired test report to the server through networking.

The application provides an enzyme-linked immunoassay analyzer 3 and an enzyme-linked immunoassay system 1, which detect absorbance of each microwell sample channel of a microwell plate 100 to be detected accommodated in a detection vacancy 420 through a movable optical detection component 4 and gradually switch detection positions. The optical detection component 4 moves relative to the microwell plate 100 to be detected, so that the displacement size required by the detection module 42 for switching the microwell detection position is effectively shortened, and the whole volume of the enzyme-linked immunoassay analyzer 3 is reduced; the position of the micro-pore plate 100 to be detected is fixed, so that liquid in a sample channel is static and does not shake, the reliability of an ELISA test result is improved due to the reduction of interference factors, and the test operation is simpler and more convenient; the arrangement of the detection vacancy 420 in the optical detection part 4 enables the whole structure of the ELISA 3 to be more compact; the light guide module 41 is connected with the detection module 42, so that the change of the light path and the light conduction are more stable, and the reliability and the accuracy of the detection result of the ELISA 3 are improved.

The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims (19)

1. An enzyme-linked immunoassay analyzer, comprising:

the optical detection component comprises a light guide module and a detection module;

the light guide module is configured to change the propagation direction of detection light, the detection module is arranged at one end of the light guide module outputting the detection light, the detection module is provided with a detection vacancy, and the detection vacancy is configured to accommodate a micro-pore plate to be detected;

the light guide module and the detection module can move relative to the micro-pore plate to be detected so as to switch the irradiation position of the detection light on the micro-pore plate to be detected.

2. The enzyme-linked immunoassay analyzer of claim 1, wherein the light guide module comprises:

an optical fiber bundle having a bundle end and a plurality of branch ends;

the optical fiber accommodating shell, the beam combining end and the branch ends are arranged on the first end face of the optical fiber accommodating shell.

3. The enzyme-linked immunoassay analyzer of claim 2, wherein each of the branched ends is of uniform height relative to the first end face.

4. The enzyme-linked immunoassay analyzer of claim 2, wherein each of the branch ends is connected to the bundle end by a bent optical fiber wire, all of which are solidified in the optical fiber housing.

5. The enzyme-linked immunoassay analyzer of claim 2, wherein the detection module comprises a sample illumination portion having the detection void; the end face of one end of the detection vacancy is provided with a plurality of light input holes, and the end face of the other end of the detection vacancy is provided with a plurality of light output holes;

the branch ends comprise a plurality of sample detection branch ends, one sample detection branch end is accommodated at one end of one light input hole, and one light input hole coincides with the central axis of one light output hole.

6. The enzyme-linked immunoassay analyzer of claim 5, wherein the detection module further comprises a sample detection plate disposed at one end of the sample illumination portion and covering all of the light output apertures.

7. The enzyme-linked immunoassay analyzer of claim 5, wherein the branch end further comprises a reference detection branch end, the reference detection branch end being disposed on one side of the sample detection branch end;

the detection module further includes a reference detection plate coupled to the first end face, the reference detection plate configured to: and detecting the light intensity of the detection light output by the reference detection branch end.

8. The enzyme-linked immunoassay analyzer of claim 5, wherein the detection module further comprises a plano-convex lens, the plano-convex lens is disposed at the other end of the light input hole, and the convex surface of the plano-convex lens faces the detection vacancy.

9. The enzyme-linked immunoassay analyzer of claim 5, wherein the detection module further comprises a lenticular lens disposed at an end of the light output aperture proximate to the detection aperture.

10. The enzyme-linked immunoassay analyzer of claim 1, further comprising:

the micropore plate to be tested is fixed in the shell;

and the transmission part is arranged in the shell and is configured to pull the detection module or the light guide module to move.

11. The enzyme-linked immunoassay analyzer of claim 10, wherein the transmission member comprises:

the synchronous belt is connected with the detection module or the light guide module;

and the motor output end is connected with the synchronous belt.

12. The enzyme-linked immunoassay analyzer of claim 11, wherein the transmission member further comprises:

at least one guide rail connected with the housing;

the sliding block is movably arranged on the guide rail and is connected with the light guide module or the detection module.

13. The enzyme-linked immunoassay analyzer of claim 10, wherein the housing comprises a microplate holder having a microplate receiving well and at least one clip;

one clamping piece is arranged at one end of the micropore plate accommodating groove, and the micropore plate to be tested is detachably arranged in the micropore plate accommodating groove through the clamping piece.

14. The enzyme-linked immunoassay analyzer of claim 1, wherein the optical detection assembly further comprises:

the light source module is arranged on one side of the light guide module, and the detection light output by the light source module irradiates on the light receiving end face of the light guide module.

15. The enzyme-linked immunoassay analyzer of claim 14, wherein the light source module comprises:

at least one light emitting element;

at least one optical filter, one optical filter is arranged at the light output end of one light emitting element, and the optical filter is configured to: and screening out light rays with specified wavelengths as the detection light.

16. The enzyme-linked immunoassay analyzer of claim 15, wherein the plurality of light emitting elements are provided, and the light outputted from each light emitting element is collected at a light crossing point after being filtered by the optical filter; the center point of the light receiving end face coincides with the light crossing point.

17. The enzyme-linked immunoassay analyzer of claim 15, wherein the light emitting element is a monochromatic diode LED lamp and the filter is a narrowband filter.

18. The enzyme-linked immunoassay analyzer according to any of claims 1-17, further comprising:

the control part, the control part includes control module and communication module, control module with detection module communication module electric connection, communication module is configured as: wired and/or wireless connection with the user terminal.

19. An enzyme-linked immunoassay system, wherein the enzyme-linked reaction assay system comprises:

at least one enzyme linked immunoassay according to claim 18;

and the user terminal is connected with each ELISA analyzer through the communication module.