CN114258757B - Germination accelerating method for rose seeds - Google Patents
Germination accelerating method for rose seeds Download PDFInfo
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- CN114258757B CN114258757B CN202111678909.9A CN202111678909A CN114258757B CN 114258757 B CN114258757 B CN 114258757B CN 202111678909 A CN202111678909 A CN 202111678909A CN 114258757 B CN114258757 B CN 114258757B
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Abstract
The invention discloses a germination accelerating method of rose seeds. The germination accelerating method comprises the following steps: drying rose seeds at the temperature of 1 for 3-5 hours; soaking the seeds in water at the temperature of 2 for 1 to 1.5 hours after drying; soaking the seeds in water at the temperature of 3 for 17.5-21 hours; taking out the seeds, and drying at the temperature of 3-5 h; soaking the seeds in water at the temperature of 3 for 17.5-21 hours; repeating the step S2 for 3-4 times; the temperature 1 is 24-28 ℃; the temperature 2 is 38-42 ℃; the temperature 3 is 24-28 ℃. The invention carries out systematic research and design aiming at the germination conditions of rose seeds, and utilizes a temperature changing method and a dry-wet alternating method to carry out germination accelerating, thereby not only remarkably improving the germination rate and shortening the germination time, but also ensuring that the seeds are not damaged in the whole process, and the treated seeds can be sowed to normally emerge and normally grow and develop. The germination accelerating method provided by the invention has the advantages of mild condition, simple steps, easiness in operation and good germination accelerating effect, and is suitable for the scale propagation of roses.
Description
Technical Field
The invention relates to the field of seed germination, in particular to a germination accelerating method of rose seeds.
Background
The cherokee rose root is a plurality of medicinal materials commonly used by minority families such as southwest Yao nationality, miao nationality, zhuang medicine, tujia and the like in China, has the effects of clearing heat and promoting diuresis, removing toxin and detumescence, promoting blood circulation and stopping bleeding, and astringing and inducing astringency, and has excellent ornamental value and application value besides medicinal value. The product is dried root and stem of Rosa cymosatratt and Rosa multiflora var. Cathayensis Rehder & E.H.Wilson, rosa Rosa roxburghii of Rosa of Rosaceae, and Rosa Rosa is climbing shrub, and as the demand of Rosa plant resource in the market increases, the wild Rosa plant resource is in demand, and the development of artificial planting technology is forced.
At present, in actual production, asexual propagation modes such as cuttage, layering or grafting are mainly adopted to propagate roses. Although the cultivation and growth period of asexual propagation seedlings is short, germplasm degeneration can be caused for a long time. The seedlings of the seeds have the advantages of strong adaptability and easy domestication, and have important value for fine variety breeding. However, the rose seeds generally have the problems of difficult germination, weak seedlings and the like, and restrict the production of seedlings. Therefore, research on germination characteristics of rose seeds is needed, seed germination obstacle factors are ascertained, obstacle of seed germination is relieved, and germination rate and seedling rate of the rose seeds are promoted and improved.
Disclosure of Invention
In order to solve the problems of difficult rose cultivation and difficult seed germination, the invention provides a germination accelerating method for rose seeds.
The invention aims to provide a germination accelerating method of rose seeds.
In order to achieve the above object, the present invention is realized by the following means:
a germination accelerating method of rose seeds, comprising the following steps:
s1, drying rose seeds at a temperature of 1 for 3-5 hours; soaking the seeds in water at the temperature of 2 for 0 to 1.5 hours after drying; soaking the seeds in water at the temperature of 3 for 17.5-21 hours;
s2, taking out the seeds, and drying at the temperature of 3-5 hours; soaking the seeds in water at the temperature of 3 for 17.5-21 hours;
s3, repeating the step S2 for 3-4 times;
the temperature 1 is 24-28 ℃; the temperature 2 is 38-42 ℃; the temperature 3 is 24-28 ℃.
Preferably, in step S1, the rose-seeds are dried for 5 hours at a temperature 1, said temperature 1 being 26 ℃.
Preferably, in step S1, the seeds are immersed for 1h in water at a temperature of 2 after drying, said temperature 2 being 40 ℃.
Preferably, in step S1, the seeds are immersed in water at a temperature 3 for 18 hours, said temperature 3 being 26 ℃.
Preferably, in step S2, the seeds are removed and dried at a temperature of 3 for 5 hours, said temperature 3 being 26 ℃.
Preferably, in step S2, the seeds are immersed in water at a temperature 3 for 19h, said temperature 3 being 26 ℃.
Preferably, in step S3, step S2 is repeated 3 times.
Preferably, the germination accelerating method further comprises the step S4 of soaking the seeds in gibberellin for 24-48 hours.
Preferably, in step S4, the seeds are placed in gibberellin for 48 hours.
Preferably, in the step S4, the concentration of the gibberellin is 1000mg/L to 2000mg/L.
More preferably, in step S4, the gibberellin concentration is 2000mg/L.
After the rose seeds are treated by the germination accelerating method provided by the invention, the rose seeds can be sown in a matrix for continuous growth.
Preferably, the seeding mode is on-demand.
Preferably, the matrix is vermiculite.
Preferably, the rose seeds comprise one or more of cherokee rose seeds, roselle rose seeds and/or small fruit rose seeds.
Compared with the prior art, the invention has the following beneficial effects:
the invention carries out systematic research and design aiming at the germination conditions of rose seeds, and utilizes a temperature changing method and a dry-wet alternating method to carry out germination accelerating, thereby not only remarkably improving the germination rate and shortening the germination time, but also ensuring that the seeds are not damaged in the whole process, and the treated seeds can be sowed to normally emerge and normally grow and develop. The germination accelerating method provided by the invention has the advantages of mild condition, simple steps, easiness in operation and good germination accelerating effect, and is suitable for the scale propagation of roses.
Detailed Description
The invention will be further described in detail in connection with the following description and specific examples, which are intended to be illustrative of the invention and are not intended to limit the scope of the invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials, reagents and the like used, unless otherwise specified, are those commercially available.
The seed collection and preservation method used in the invention comprises the following steps: in 12 months 2019, cherokee rose seed, roselle rose seed and small fruit rose seed with seed coat are collected from the Hunan Ji, fruits are broken manually, the seeds are taken out and soaked in clear water, the floating seeds are removed, the sinking full seeds are collected, and the seeds are dried in the shade and stored at 4 ℃.
Example 1 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) Drying in an oven at 40 ℃ for 5 hours, and soaking in clean water at 26 ℃ for 19 hours. The drying and soaking operations were repeated 4 times in total.
(2) The rose seeds were soaked with 2000mg/mL gibberellin (GA 3) for 48h.
Example 2 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And the mixture is placed in an indoor environment at 26 ℃ for blowing and drying for 5 hours. After drying, the seeds are soaked in clear water at 40 ℃ for 1h, and then soaked in clear water at 26 ℃ for 18h. After soaking, the seeds are placed in indoor environment at 26 ℃ for drying for 5 hours by blowing, and then placed in clean water at 26 ℃ for soaking for 19 hours. The operations of drying at 26℃for 5 hours and immersing at 26℃for 19 hours were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Example 3 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) Drying in an oven at 40 ℃ for 5 hours. After drying, the seeds were immersed in clear water at 26℃for 19h. After soaking, the seeds are placed in indoor environment at 26 ℃ for drying for 5 hours by blowing, and then placed in clean water at 26 ℃ for soaking for 19 hours. The operations of drying at 26℃for 5 hours and immersing at 26℃for 19 hours were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Example 4 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And (5) drying the mixture by blowing in an indoor environment at 24 ℃ for 5 hours. After drying, the seeds are soaked in clear water at 38 ℃ for 1h, and then soaked in clear water at 24 ℃ for 18h. After soaking, the seeds are placed in indoor environment at 24 ℃ for drying for 5 hours by blowing, and then placed in clean water at 24 ℃ for soaking for 19 hours. The operations of drying at 24℃for 5h and soaking at 24℃for 19h were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Example 5 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And the mixture is placed in an indoor environment at 28 ℃ for drying for 5 hours by blowing. After drying, the seeds are soaked in clear water at 42 ℃ for 1h, and then soaked in clear water at 28 ℃ for 18h. After soaking, the seeds are placed in an indoor environment at 28 ℃ for drying for 5 hours by blowing, and then placed in clean water at 28 ℃ for soaking for 19 hours. The drying at 28℃for 5h and the soaking at 28℃for 19h were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Comparative example 1 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) Soaking in clear water at 40deg.C for 5 hr, and soaking in clear water at 26deg.C for 19 hr. The soaking operation at 40℃for 5 hours and the soaking operation at 26℃for 19 hours were repeated 4 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Comparative example 2 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) Drying in an oven at 40 ℃ for 5 hours, and then drying in an indoor environment at 26 ℃ for 19 hours. The operations of drying at 40℃for 5 hours and at 26℃for 19 hours were repeated 4 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Comparative example 3 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And the mixture is placed in an indoor environment at 26 ℃ for blowing and drying for 5 hours. After drying, the seeds are soaked in clear water at 45 ℃ for 1h, and then soaked in clear water at 26 ℃ for 18h. After soaking, the seeds are placed in indoor environment at 26 ℃ for drying for 5 hours by blowing, and then placed in clean water at 26 ℃ for soaking for 19 hours. The operations of drying at 26℃for 5 hours and immersing at 26℃for 19 hours were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Comparative example 4 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) Firstly, placing the mixture in an indoor environment at 26 ℃ to be blown and dried for 5 hours, and then placing the mixture in clean water at 26 ℃ to be soaked for 19 hours. The drying and soaking operations were repeated 4 times in total.
(2) The rose seeds were soaked with 2000mg/mLGA3 for 48 hours.
Comparative example 5 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And (5) drying the mixture by blowing in an indoor environment at 30 ℃ for 5 hours. After drying, the seeds are soaked in clear water at 36 ℃ for 1h, and then soaked in clear water at 30 ℃ for 18h. After soaking, the seeds are placed in an indoor environment at 30 ℃ to be blown and dried for 5 hours, and then placed in clear water at 30 ℃ to be soaked for 19 hours. The drying at 30℃for 5h and the soaking at 30℃for 19h were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Comparative example 6 method for accelerating germination of rose seeds
Taking 1000 cherokee rose seeds, 1000 rosette rose seeds and 1000 small fruit rose seeds, and treating the seeds by the following method:
(1) And the mixture is placed in an indoor environment at 36 ℃ for blowing and drying for 5 hours. After drying, the seeds are soaked in clear water at 30 ℃ for 1h, and then soaked in clear water at 36 ℃ for 18h. After soaking, the seeds are placed in 36 ℃ indoor environment for drying for 5 hours by blowing, and then placed in 36 ℃ clean water for soaking for 19 hours. The operations of drying at 36℃for 5 hours and immersing at 36℃for 19 hours were repeated 3 times in total.
(2) The rose seeds were soaked with 2000mg/mL GA3 for 48h.
Influence of different methods of application on Rosa seed germination
Cherokee rose seed, roselle rose seed and small fruit rose seed were treated by the methods of examples 1 to 5 and comparative examples 1 to 7, respectively.
Taking out the treated cherokee rose seed, roselle rose seed and small fruit rose seed from GA3, washing with clear water, respectively spreading in vermiculite, and culturing at 26 deg.C.
Counting germination rate (G) and Germination Vigor (GV) of cherokee rose seed, roselle rose seed, wherein the specific calculation formula is as follows:
germination rate g=ga/gn×100% (GA is the number of seeds that sprout normally, GN is the total number of seeds);
the number of days in which the number of germinated seeds reached the highest peak period during germination was recorded, and the vigour gv=gt/gn×100% (GT is the number of germinated seeds at the time when germination reached the peak period, GN is the total number of seeds).
2. Experimental results
The cherokee rose seed, the rosette rose seed and the small fruit rose seed are treated by the methods of examples 1 to 5 and comparative examples 1 to 6, and three roses can normally emerge and grow after sowing.
The statistical results of germination percentage (G) and Germination Index (GI) of cherokee rose seed, roselle rose seed, and rose seed are shown in tables 1 to 3.
TABLE 1 Effect of different methods on cherokee rose seed germination
TABLE 2 Effect of different methods on germination of rosemary seed
TABLE 3 Effect of different methods on seed germination of Rosa canina
As can be seen from Table 1, the germination rate of rose seeds was significantly improved by treating the rose seeds in the methods of examples 1 to 5, as compared with comparative examples 1 to 6.
Wherein, in examples 1 to 5, the germination rate of rose seeds treated by the method of example 2 was the highest, the germination rate of cherokee rose seeds was 53.67% + -0.81%, the germination rate of rosette rose seeds was 24.10% + -0.96%, and the germination rate of rose seeds was 20.97% + -0.90%.
In comparative examples 1 to 7, the treatment with the highest germination rate of cherokee rose seed was comparative example 6, the germination rate was 38.93% + -0.51%, and 5.27% lower than that of example 1 with the lowest germination rate of cherokee rose seed; the treatment with the highest germination rate of the roselle seed is comparative example 5, the germination rate is 12.77% ± 0.71%, which is 0.6% lower than the germination rate of example 1 with the lowest germination rate of the roselle seed; the treatment with the highest germination rate of the rose-hip seeds was comparative example 5, the germination rate was 14.10% ± 0.60%, and 3.63% lower than that of example 1 with the lowest germination rate of the rose-hip seeds.
The results show that the method provided by the invention can obviously promote the germination of rose seeds, shorten the cultivation time and improve the efficiency of artificially cultivating cherokee rose, roses with powder clusters and small-fruit roses.
It should be noted that the above embodiments are merely for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and that other various changes and modifications can be made by one skilled in the art based on the above description and the idea, and it is not necessary or exhaustive to all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.
Claims (1)
1. A method for accelerating germination of rose seeds, comprising the steps of:
s1, placing rose seeds in an indoor environment at 26 ℃ for blowing and drying for 5 hours; soaking the dried seeds in water at 40 ℃ for 1h; soaking the seeds in water at 26 ℃ for 18 hours;
s2, taking out seeds, and drying the seeds for 5 hours by blowing air in an indoor environment at 26 ℃; soaking the seeds in water at 26 ℃ for 19h;
s3, repeating the step S2 for 3 times;
s4, soaking the seeds in 2000mg/L gibberellin for 48 hours;
the rose seed comprises one or more of cherokee rose seed, roselle rose seed and/or small fruit rose seed.
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| CH639824A5 (en) * | 1979-02-13 | 1983-12-15 | Jelitto Klaus R Fa | Method for improving the germination of seeds where germination is a problem |
| JP4404691B2 (en) * | 2004-05-27 | 2010-01-27 | 株式会社小島組 | Seeding seed formation method and germination apparatus |
| CN101816228B (en) * | 2010-04-15 | 2011-07-27 | 浙江大学 | Method for improving vital indexes of gourd seeds |
| KR101246135B1 (en) * | 2010-11-09 | 2013-03-21 | 전남대학교산학협력단 | Method for germination improvement of genus rosa spp. |
| CN103988608B (en) * | 2014-04-21 | 2016-02-17 | 中国科学院成都生物研究所 | A kind ofly promote the method that how luxuriant Seeds of Rosa Spp germinates |
| KR101608344B1 (en) * | 2015-07-15 | 2016-04-01 | 김영강 | Method for cultivating White Korean rosebay seedling |
| CN107182501B (en) * | 2017-06-07 | 2020-12-22 | 河北省农林科学院旱作农业研究所 | Cultivation method for improving yield of strong gluten wheat |
| CN111543137B (en) * | 2020-05-30 | 2022-07-05 | 江苏嘉润生态苗木有限公司 | Rose seed seedling raising method |
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