CN114252422B - Multimode fluorescence immunoassay appearance - Google Patents

Multimode fluorescence immunoassay appearance Download PDF

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Publication number
CN114252422B
CN114252422B CN202111570710.4A CN202111570710A CN114252422B CN 114252422 B CN114252422 B CN 114252422B CN 202111570710 A CN202111570710 A CN 202111570710A CN 114252422 B CN114252422 B CN 114252422B
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Prior art keywords
dropper
linear module
shell
reagent
detection strip
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CN202111570710.4A
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CN114252422A (en
Inventor
贾俊玉
周洁
李刚
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Nanjing Changjian Bio Tech Co ltd
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Nanjing Changjian Bio Tech Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention discloses a multimode fluorescence immunoassay instrument which comprises a shell, wherein an operation panel is obliquely arranged at the front end of the shell, a detection strip socket is arranged on the front end face of the shell at the lower side of the operation panel, a detection strip conveying device is arranged at the inner side of the detection strip socket, a reagent conveying device is arranged at the inner side of the detection strip conveying device and positioned in the shell, an electric rotating disc is arranged at the top of the shell, a plurality of reagent bottle holes are formed in the electric rotating disc, reagent bottles are placed in the reagent bottle holes, and a dropper is inserted at the lower end of each reagent bottle. This multimode fluorescence immunoassay appearance through setting up detection strip conveyor and reagent conveyor, can be with the reagent on the electric turntable carousel automatic feeding in proper order to wait to detect the detection strip on, need not artifical the interpolation, very convenient, practical, a plurality of detection strips detect simultaneously moreover, and detection efficiency is high.

Description

Multimode fluorescence immunoassay appearance
Technical Field
The invention belongs to the technical field of fluorescence immunoassay, and particularly relates to a multimode fluorescence immunoassay instrument.
Background
The fluorescent immunodetection technology has the advantages of strong specificity, high sensitivity, good practicability and the like, so that the fluorescent immunodetection technology is used for measuring bioactive compounds with very low content, such as proteins (enzymes, acceptors and antibodies), hormones (steroid compounds, thyroid hormone and phthalein hormone), medicines, microorganisms and the like. Therefore, the method has wide application prospect in the detection fields of microorganism, virus antigen or antibody detection, hormone detection, tumor marker detection, drug heroin, morphine, shaking pills, ketamine and the like;
However, the existing fluorescence immunoassay equipment only can detect one sample at one side, has low detection efficiency, high manual participation and inconvenient use.
Disclosure of Invention
The present invention is directed to a multimode fluorescence immunoassay apparatus, which solves the problems set forth in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a multimode fluorescence immunoassay appearance, includes the shell, the front end slope of shell sets up operating panel, set up the test strip socket on the shell front end face of operating panel downside, the test strip socket inboard is equipped with test strip conveyor, test strip conveyor's inboard and the inside that is located the shell are equipped with reagent conveyor, the top of shell is equipped with electric turntable, set up a plurality of reagent bottle holes on the electric turntable, place the reagent bottle in the reagent bottle hole, the drip tube is pegged graft to reagent bottle lower extreme.
Preferably, the operation panel is provided with a display screen, keys and a printing paper outlet, and the printer is arranged inside the shell and positioned at the inner side of the printing paper outlet.
Preferably, a rubber plug is arranged at the lower end of the reagent bottle, and the dropper is inserted into the rubber plug.
Preferably, a check ring is arranged in the hole of the reagent bottle, and the inner diameter of the check ring is larger than the diameter of the largest part of the dropper.
Preferably, the detection strip conveying device comprises a track groove, a movable socket, a driving belt and a driving motor, wherein the movable socket is arranged on the track groove in a sliding mode, an opening is formed in the side wall of the track groove, the driving belt is arranged in the opening, the driving motor is driven to be connected with the driving belt, and the side face of the movable socket is fixedly connected with the driving belt.
Preferably, the reagent conveying device comprises a transverse linear module, a supporting slide block, a supporting disc, a longitudinal support and a longitudinal linear module, wherein the supporting slide block is arranged on the transverse linear module and is driven to transversely move by the transverse linear module, the transverse linear module is rotatably arranged on the supporting slide block, the supporting disc is rotatably arranged on the supporting slide block and is internally provided with a rotating motor, the rotating motor is driven to rotate by the rotating motor, the longitudinal linear module is arranged on the side face of the longitudinal support, a swivel is rotatably arranged on the longitudinal support, the swivel is connected with a rotation control motor outside the longitudinal support, a connecting rod is arranged on the curved surface of the swivel, and the tail end of the connecting rod is connected with a dropper clamping jaw and a dropper extrusion clamping jaw.
Preferably, the output shaft of the rotation control motor penetrates through the swivel and is rotationally connected with the output end of the longitudinal linear module, and the output shaft of the rotation control motor is fixedly connected with the swivel.
Preferably, a dropper conveying linear module is arranged at one side of the reagent conveying device and at the bottom of the inner cavity of the shell, and a dropper accommodating box is arranged on the dropper conveying linear module.
Preferably, a dropper accommodating box outlet is formed in one end of the dropper conveying linear module and located on the side wall of the shell, and the dropper accommodating box is clamped on the output end of the dropper conveying linear module.
The invention has the technical effects and advantages that: this multimode fluorescence immunoassay appearance through setting up detection strip conveyor and reagent conveyor, can be with the reagent on the electric turntable carousel automatic feeding in proper order to wait to detect the detection strip on, need not artifical the interpolation, very convenient, practical, a plurality of detection strips detect simultaneously moreover, and detection efficiency is high.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of a partial cross-sectional structure of the present invention;
FIG. 3 is a schematic diagram of a test strip transport apparatus according to the present invention;
FIG. 4 is a schematic view of the structure of the reagent delivering apparatus of the present invention;
FIG. 5 is an enlarged schematic view of the structure of the present invention at A;
FIG. 6 is a schematic view of the structure of the reagent bottle and the dropper of the present invention.
In the figure: 1. a housing; 2. an operation panel; 3. an electric turntable; 4. a test strip transport device; 41. a track groove; 42. a mobile socket; 43. a transmission belt; 44. a drive motor; 5. a reagent delivery device; 51. a transverse linear module; 52. a support slider; 53. a support disc; 54. a longitudinal support; 55. a swivel; 56. a rotation control motor; 57. a connecting rod; 58. a dropper clamping jaw; 59. the dropper extrudes the clamping jaw; 510. a longitudinal linear module; 6. a dropper conveying linear module; 7. a dropper accommodating box; 8. a reagent bottle; 9. a rubber stopper; 10. a dropper; 11. a test strip socket.
Detailed Description
The following describes the embodiments of the present invention further with reference to the drawings. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
The invention provides a multimode fluorescence immunoassay instrument as shown in figures 1 and 2, which comprises a shell 1, wherein an operation panel 2 is obliquely arranged at the front end of the shell 1, a detection strip jack 11 is arranged on the front end surface of the shell 1 at the lower side of the operation panel 2, a detection strip is inserted from the detection strip jack 11, a detection strip conveying device 4 is arranged at the inner side of the detection strip jack 11, a reagent conveying device 5 is arranged at the inner side of the detection strip conveying device 4 and positioned in the shell 1, an electric rotary disc 3 is arranged at the top of the shell 1, a plurality of reagent bottle holes are formed in the electric rotary disc 3, and reagent bottles 8 are placed in the reagent bottle holes;
Referring to fig. 6, a dropper 10 is inserted into the lower end of a reagent bottle 8, a rubber plug 9 is disposed at the lower end of the reagent bottle 8, the dropper 10 is inserted into the rubber plug 9, a reagent or diluent is filled into the reagent bottle 8, then the dropper 10 is inserted into the rubber plug 9, the reagent or diluent in the reagent bottle 8 is introduced into the dropper 10, and then the reagent bottle is placed into a reagent bottle hole of an electric turntable 3 for use, a retainer ring is disposed in the reagent bottle hole, the retainer ring can support the reagent bottle 8, and the inner diameter of the retainer ring is larger than the maximum diameter of the dropper 10, so that the dropper 10 can pass through the retainer ring.
As shown in fig. 1, the operation panel 2 is provided with a display screen, keys and a printing paper outlet, a printer is arranged inside the casing 1 and inside the printing paper outlet, and the printer prints the detection result and spits out through the printing paper outlet.
Referring to fig. 3, the test strip conveying device 4 includes a track groove 41, a moving socket 42, a driving belt 43 and a driving motor 44, the moving socket 42 is slidably disposed on the track groove 41, an opening is formed in a side wall of the track groove 41, the driving belt 43 is disposed in the opening, rollers are disposed at inner sides of two ends of the driving belt 43 and rotatably mounted in the opening, the driving motor 44 is connected with the driving belt 43, a rotating shaft of the rollers on one side extends to an upper side of the track groove 41 and is connected with a driving box, the other end of the driving box is connected with the driving motor 44, a side surface of the moving socket 42 is fixedly connected with the driving belt 43, the driving motor 44 drives the driving belt 43 to operate, so that the moving socket 42 is driven to move, and the test strip is inserted into the moving socket 42 from the test strip jack 11 and then sent to a working position inside the casing 1.
As shown in fig. 4 and 5, the reagent conveying device 5 comprises a transverse linear module 51, a supporting slide block 52, a supporting disc 53, a longitudinal bracket 54 and a longitudinal linear module 510, wherein the supporting slide block 52 is arranged on the transverse linear module 51, the transverse linear module 51 is driven to transversely move by the transverse linear module 51, the transverse linear module 51 is rotatably arranged on the supporting slide block 52, the supporting disc 53 is rotatably arranged on the supporting slide block 52, a rotating motor is arranged in the supporting slide block 52, the rotating motor is driven to rotate by the supporting disc 53, the longitudinal linear module 510 is arranged on the side face of the longitudinal bracket 54, a rotating ring 55 is rotatably arranged on the longitudinal bracket 54, a rotating control motor 56 is arranged on the rotating ring 55 and is connected with the outer side of the longitudinal bracket 54, an output shaft of the rotating control motor 56 penetrates through the rotating ring 55 and is rotatably connected with the output end of the longitudinal linear module 510, an output shaft of the rotating control motor 56 is fixedly connected with the rotating ring 55, a connecting rod 57 is arranged on a curved surface of the rotating ring 55, and the tail end of the connecting rod 57 is connected with a dropper 58 and a dropper extrusion clamping jaw 59.
A dropper conveying linear module 6 is arranged at one side of the reagent conveying device 5 and positioned at the bottom of the inner cavity of the shell 1, and a dropper accommodating box 7 is arranged on the dropper conveying linear module 6; one end of the dropper conveying linear module 6 is provided with a dropper accommodating box taking-out port positioned on the side wall of the shell 1, and the dropper accommodating box 7 is clamped on the output end of the dropper conveying linear module 6. The used dropper is placed in the dropper holding box 7 and is transported out through the dropper transport linear module 6.
In the using process of the multi-mode fluorescence immunoassay instrument, firstly, reagents, diluents and the like which are required to be used are filled into reagent bottles 8 according to detection requirements and placed on an electric turntable 3 in sequence, then, a detection strip is inserted into a detection strip socket 11, a transmission motor 44 drives a transmission belt 43 to operate so as to drive a movable socket 42 to move, the detection strip is sent to a working position inside a shell 1, at the moment, a reagent conveying device 5 operates, a transverse linear module 51 drives a longitudinal bracket 54 and other structures to move to the lower side of the electric turntable 3, a longitudinal linear module 510 drives a rotary ring 55 and other structures to move upwards, at the moment, a connecting rod 57 and the transverse linear module 51 are in the same plane, a dropper clamping jaw 58 clamps a dropper 10 and is taken down, then, the transverse linear module 51 drives the structures such as the longitudinal bracket 54 and the like to move towards the detection strip, a rotary ring 55 is driven to rotate by rotating a control motor 56, so that a drop mouth of the clamped dropper 10 is downward, a clamping jaw 59 is pressed to press the tail end of the dropper 59, the reagent is dropped into the detection mouth of the detection strip, and the dropper 10 is placed into a storage box 7 after being used.
The detection strip conveying device 4 and the reagent conveying device 5 are arranged at the bottom of the shell 1 and at the rear side of the inner cavity, so that the design is reasonable, the available space inside the shell 1 is larger, and the layout and the installation of other equipment are facilitated.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.

Claims (5)

1. Multimode fluorescence immunoassay appearance, including shell (1), its characterized in that: the front end of the shell (1) is obliquely provided with an operation panel (2), the front end face of the shell (1) at the lower side of the operation panel (2) is provided with a detection strip insertion opening (11), the inner side of the detection strip insertion opening (11) is provided with a detection strip conveying device (4), the inner side of the detection strip conveying device (4) is provided with a reagent conveying device (5) and positioned in the shell (1), the top of the shell (1) is provided with an electric rotary table (3), a plurality of reagent bottle holes are formed in the electric rotary table (3), reagent bottles (8) are placed in the reagent bottle holes, and the lower end of each reagent bottle (8) is connected with a dropper (10) in an inserting mode;
the detection strip conveying device (4) comprises a track groove (41), a movable socket (42), a transmission belt (43) and a transmission motor (44), wherein the movable socket (42) is arranged on the track groove (41) in a sliding mode, an opening is formed in the side wall of the track groove (41), the transmission belt (43) is arranged in the opening, the transmission motor (44) is used for driving and connecting the transmission belt (43), and the side face of the movable socket (42) is fixedly connected with the transmission belt (43);
The reagent conveying device (5) comprises a transverse linear module (51), a supporting sliding block (52), a supporting disc (53), a longitudinal bracket (54) and a longitudinal linear module (510), wherein the supporting sliding block (52) is arranged on the transverse linear module (51) and is driven to transversely move through the transverse linear module (51), the transverse linear module (51) is rotatably arranged on the supporting sliding block (52), the supporting disc (53) is rotatably arranged on the supporting sliding block (52) and is internally provided with a rotating motor, the rotating motor drives the supporting disc (53) to rotate, the longitudinal linear module (510) is arranged on the side face of the longitudinal bracket (54), a rotating ring (55) is rotatably arranged on the longitudinal bracket (54), a connecting rod (57) is arranged on the curved surface of the rotating ring (55), and the tail end of the connecting rod (57) is connected with a dropper clamping jaw (58) and a dropper extrusion clamping jaw (59);
An output shaft of the rotation control motor (56) penetrates through the swivel (55) and is rotationally connected with an output end of the longitudinal linear module (510), and the output shaft of the rotation control motor (56) is fixedly connected with the swivel (55);
A dropper conveying linear module (6) is arranged at one side of the reagent conveying device (5) and positioned at the bottom of the inner cavity of the shell (1), and a dropper accommodating box (7) is arranged on the dropper conveying linear module (6);
The multimode fluorescence immunoassay instrument is characterized in that in the using process, firstly reagents and diluent to be used are filled into a reagent bottle (8) according to detection requirements and are sequentially placed on an electric rotary table (3), then a detection strip is inserted into a detection strip socket (11), a transmission motor (44) drives a transmission belt (43) to operate, so that a movable socket (42) is driven to move, the detection strip is sent to a working position inside a shell (1), a reagent conveying device (5) operates at the moment, a longitudinal support (54) structure is driven to move to the lower side of the electric rotary table (3) by a transverse linear module (51), a longitudinal linear module (510) is driven upwards, a rotating ring (55) structure is driven to move upwards, a connecting rod (57) and the transverse linear module (51) are in the same plane at the moment, a dropper clamping jaw (58) clamps and takes down the dropper (10), then the transverse linear module (51) drives the longitudinal support (54) structure to move towards the detection strip, a rotary control motor (56) is driven to rotate, a dropper clamping jaw (10) is enabled to move downwards, and the dropper clamping jaw (10) is pressed to press a dropper clamping jaw (59) to press the detection strip (10) to place the detection box (7).
2. The multimode fluoroimmunoassay instrument of claim 1, wherein: the operation panel (2) is provided with a display screen, keys and a printing paper outlet, and a printer is arranged inside the shell (1) and positioned at the inner side of the printing paper outlet.
3. The multimode fluoroimmunoassay instrument of claim 1, wherein: the lower end of the reagent bottle (8) is provided with a rubber plug (9), and the dropper (10) is inserted into the rubber plug (9).
4. The multimode fluoroimmunoassay instrument of claim 1, wherein: a check ring is arranged in the hole of the reagent bottle, and the inner diameter of the check ring is larger than the diameter of the largest part of the dropper (10).
5. The multimode fluoroimmunoassay instrument of claim 1, wherein: one end of the dropper conveying linear module (6) is provided with a dropper accommodating box taking-out port located on the side wall of the shell (1), and the dropper accommodating box (7) is clamped on the output end of the dropper conveying linear module (6).
CN202111570710.4A 2021-12-21 2021-12-21 Multimode fluorescence immunoassay appearance Active CN114252422B (en)

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CN114252422B true CN114252422B (en) 2024-04-30

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105929184A (en) * 2016-06-30 2016-09-07 嘉兴凯实生物科技有限公司 Full-automatic luminescence immunity analyzer
CN109900892A (en) * 2019-04-22 2019-06-18 广西埃韦迪生物科技有限公司 A kind of medical rotary table formula entry fluorescence immunity analyzer
CN110118878A (en) * 2019-06-19 2019-08-13 丹娜(天津)生物科技有限公司 A kind of Biochemical Analyzer
CN210128976U (en) * 2019-06-29 2020-03-06 重庆市璧山区人民医院 Urine sugar detection device that physical examination doctor used
WO2021180108A1 (en) * 2020-03-11 2021-09-16 Guizhou Meixinda Medical Technologies Co., Ltd. Devices and components for automated tissue processing and staining and uses thereof
CN113804674A (en) * 2021-09-18 2021-12-17 广州蓝勃生物科技有限公司 Reagent frame and chemiluminescence immunoassay appearance

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105929184A (en) * 2016-06-30 2016-09-07 嘉兴凯实生物科技有限公司 Full-automatic luminescence immunity analyzer
CN109900892A (en) * 2019-04-22 2019-06-18 广西埃韦迪生物科技有限公司 A kind of medical rotary table formula entry fluorescence immunity analyzer
CN110118878A (en) * 2019-06-19 2019-08-13 丹娜(天津)生物科技有限公司 A kind of Biochemical Analyzer
CN210128976U (en) * 2019-06-29 2020-03-06 重庆市璧山区人民医院 Urine sugar detection device that physical examination doctor used
WO2021180108A1 (en) * 2020-03-11 2021-09-16 Guizhou Meixinda Medical Technologies Co., Ltd. Devices and components for automated tissue processing and staining and uses thereof
CN113804674A (en) * 2021-09-18 2021-12-17 广州蓝勃生物科技有限公司 Reagent frame and chemiluminescence immunoassay appearance

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