CN114230253B - Sea sand solidified body and preparation method and application thereof - Google Patents

Sea sand solidified body and preparation method and application thereof Download PDF

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CN114230253B
CN114230253B CN202111636735.XA CN202111636735A CN114230253B CN 114230253 B CN114230253 B CN 114230253B CN 202111636735 A CN202111636735 A CN 202111636735A CN 114230253 B CN114230253 B CN 114230253B
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刘亚茹
李书鹏
郭丽莉
熊静
王祺
丛欣江
汪福旺
张家铭
李嘉晨
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BCEG Environmental Remediation Co Ltd
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Abstract

The invention discloses a preparation method of a sea sand solidified body, which comprises the steps of filling sea sand into a mould with openings at two ends, and sealing two ends of the mould by using a filter disc; sequentially pouring the bacterial liquid, the stationary liquid and the cementing liquid into a mould in batches, and demoulding and maintaining to obtain sand columns; the bacterial liquid comprises a strain capable of producing urease, wherein the strain is domesticated by urea and sodium chloride; the filling speed of the bacterial liquid is 0.24-0.5mL/min; the injection speed of the stationary liquid is 0.24-0.5mL/min; the filling speed of the cementing liquid is 0.04-0.2mL/min. The sea sand solidified body is prepared by the preparation method of the sea sand solidified body and is directly applied to engineering construction as a building material. The invention can realize the solidification of the sea sand without using a high-pressure reaction kettle for heating, and does not need to consume higher energy, thereby greatly reducing the difficulty of the solidification of the sea sand and simultaneously improving the strength of a sea sand solidification body.

Description

Sea sand solidified body and preparation method and application thereof
Technical Field
The invention relates to the technical field of sea sand solidification, in particular to a sea sand solidification body and a preparation method and application thereof.
Background
A large amount of building materials are needed in the island construction process, and the building materials for island construction in China mainly depend on inland transportation, so that the island construction cost is very high, and the construction time cost is greatly prolonged. China is rich in sea sand resources, and the storage capacity of only offshore sea sand is very large.
Because the content of chloride ions in the sea sand is high, and the chloride ions can erode steel bars to bring potential safety hazards to engineering, the sea sand is difficult to be applied to engineering construction such as island construction, coastline restoration and the like. Researchers find that sea sand can be directly applied to engineering construction as a building material by using a hydrothermal curing technology to cure the sea sand into rock. However, the hydrothermal method for sea sand solidification needs to use a high-pressure reaction kettle, and hydrothermal solidification is carried out at a higher temperature, so that higher energy is consumed for sea sand solidification, and the difficulty in sea sand solidification is greatly increased.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the defects that in the prior art, a high-pressure reaction kettle is needed to be used for hydrothermal solidification of sea sand by a hydrothermal method, and the solidified sea sand needs to consume higher energy at higher temperature, so that the sea sand solidification cost is increased, and the sea sand solidification difficulty is greatly increased.
Therefore, the invention provides a preparation method of a sea sand solidified body, which comprises the following steps:
filling sea sand into the mold with openings at two ends, and sealing two ends of the mold by using a filter sheet; sequentially pouring the bacterial liquid, the stationary liquid and the cementing liquid into a mould filled with sea sand in batches, and demoulding and maintaining to obtain sand columns; filling the bacterial liquid, the stationary liquid and the cementing liquid once per cycle into one batch;
the bacterial liquid comprises a strain capable of producing urease, wherein the strain is domesticated by urea and sodium chloride; the filling speed of the bacterial liquid is 0.24-0.5mL/min; the injection speed of the stationary liquid is 0.24-0.5mL/min; the pouring speed of the cementing liquid is 0.04-0.2mL/min;
OD of the bacterial liquid 600 =0.8-1.2; the concentration of the stationary liquid is 0.03-0.08mol/L; the concentration of the cementing liquid is 0.5-1mol/L.
Optionally, the volume ratio of the bacterial liquid poured into each batch to the sea sand in the mould is (5-8) to 12; the volume ratio of the fixing liquid filled in each batch to the sea sand in the mould is (1-2) to 12; the volume ratio of the cementing liquid to the sea sand in the mould poured in each batch is (25-50): 12.
Optionally, the fixing solution is a calcium chloride solution.
Optionally, the cementing liquid is a mixed liquid of calcium chloride and urea, and the molar ratio of the calcium chloride to the urea is 1.
Optionally, the strain is carbonate mineralization strain.
Optionally, the bacteria liquid obtaining method includes:
inoculating the strain into a liquid culture medium, and culturing at 28-32 deg.C for 24-48 h.
Optionally, the liquid culture medium comprises 18-25g/L of yeast extract, 8-12g/L of ammonium sulfate and 13-16g/L of Tris (Tris).
Optionally, after the strain is domesticated, the salinity tolerance is improved to 30-35g/L, and the urea decomposition capacity is improved to 75-85g/L.
Optionally, after the bacterial liquid is poured, standing for 2-3h, and then pouring the stationary liquid.
The invention provides a sea sand solidified body which is prepared by the preparation method of the sea sand solidified body.
The sea sand solidified body is directly applied to engineering construction as a building material.
The technical scheme of the invention has the following advantages:
1. the invention provides a preparation method of a sea sand solidified body, which improves the salinity resistance of a bacterial liquid through domestication, so that the bacterial liquid can survive in sea sand with higher chloride ion content, and a bacterial strain capable of decomposing urease can decompose urea in the sea sand, generates calcium carbonate deposition with calcium ions in the sea sand, and solidifies the sea sand into hard rock under the combined action of a fixing liquid and a cementing liquid. The sea sand can be cured without heating a high-pressure reaction kettle, so that the sea sand is cured without consuming higher energy, and the difficulty of sea sand curing is greatly reduced. In addition, the strength of the sand column obtained after sea sand solidification is greatly improved through the combined action of the bacterial liquid, the fixing liquid and the cementing liquid.
2. According to the preparation method of the sea sand solidified body, provided by the invention, the salinity resistance of the bacterial strain and the decomposition capability of urea are controlled by controlling the volume ratio of the bacterial liquid, the stationary liquid and the cementing liquid to the sea sand, and the grouting batch is controlled, so that the strength of the solidified body after the sea sand is solidified is improved to 34.7MPa, and the strength of the sea sand solidified body is greatly improved.
3. The preparation method of the sea sand solidified body provided by the invention has the advantages that the operation process is simple, the relatively complex solidification condition is not required to be provided, the strength of the sea sand solidified body obtained by solidification is relatively high, the sea sand solidified body can be applied to the fields of coastline restoration, military defense engineering, artificial island reef construction and the like, and the utilization cost of the sea sand is greatly reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a flow chart of a method of solidifying sea sand in an embodiment of the present invention;
fig. 2 is a schematic structural view of a mold in an embodiment of the present invention.
Reference numerals: 1. an acrylic tube; 2. sea sand; 3. a filter disc; 4. an organic glass plate; 5. a rubber plug; 6. a first reducer pipe; 7. a second reducer pipe.
Detailed Description
The following examples are provided to better understand the present invention, not to limit the best mode, and not to limit the content and protection scope of the present invention, and any product that is the same or similar to the present invention and is obtained by combining the present invention with other features of the prior art and the present invention falls within the protection scope of the present invention.
The examples do not show the specific experimental steps or conditions, and can be performed according to the conventional experimental steps described in the literature in the field. The reagents or instruments used are not indicated by manufacturers, and are all conventional reagent products which can be obtained commercially.
The salinity tolerance of the strain in this example means that the strain is cultured in a medium with a certain concentration of sodium chloride, and if the number of viable bacteria in the medium is more than 10 8 cfu/mL, then a certain value is the salinity tolerance of the strain;
the urea decomposition ability of the strain in this example means that the strain is cultured in a medium containing urea at a certain concentration, and if the number of viable bacteria in the medium is greater than 10 8 cfu/mL, the "certain value" is the urea decomposition ability of the strain.
The carbonate mineralization bacteria is Bacillus pasteurii with a deposit number ATCC 11895 and purchased from American type culture Collection.
The yeast extract was purchased from Oboxing Biotechnology, inc., beijing.
The sea sand is from Hainan area, and has particle size of 0.25-0.5 mm.
Example 1
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: domesticating Bacillus pasteurii in a culture medium with urea concentration of 20g/L and sodium chloride concentration of 10g/L for 24h, sequentially increasing the sodium chloride concentration in the culture medium to 20g/L, 25g/L, 32g/L and 35g/L, and continuing domestication until the viable count is more than 10 when the sodium chloride concentration in the culture medium is 35g/L 8 Sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L, 75g/L and 80g/L for continuous domestication until the viable count is more than 10 when the concentration of urea in the culture medium is 80g/L 8 Stopping domestication of cfu/mL;
obtaining a bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 30 ℃ for 36 h; the liquid culture medium comprises agar 20g/L, yeast extract 20g/L, ammonium sulfate 10g/L, tris 15.748g/L;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =1.0, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 2 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1), pouring a calcium chloride solution (stationary liquid) with the concentration of 0.05mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 2;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1:1;
(1) the grouting process of (1) - (3) is a batch grouting process, and after the first batch grouting process is carried out, two batches of grouting processes are carried out according to the steps (1) - (3);
and (5) maintenance: and (3) demolding the acrylic tube after grouting to obtain sand columns, soaking the sand columns in deionized water for 36 hours, and drying the soaked sand columns at 65 ℃ for 36 hours to realize the solidification of the sea sand.
Example 2
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: bacillus pasteurii in a medium with urea concentration of 20g/L and sodium chloride concentration of 10g/LDomesticating for 24h, sequentially increasing the concentration of sodium chloride in the culture medium to 20g/L, 25g/L, 32g/L and 35g/L, and continuing domestication until the viable count is more than 10 when the concentration of sodium chloride in the culture medium is 35g/L 8 cfu/mL, sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L, 75g/L and 80g/L, and continuing to acclimatize until the viable count is more than 10 when the concentration of urea in the culture medium is 80g/L 8 Stopping domestication of cfu/mL;
obtaining bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 30 ℃ for 36 h; the liquid culture medium comprises agar 20g/L, yeast extract 20g/L, ammonium sulfate 10g/L, tris 15.748g/L;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =1.0, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 2 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1) is finished, filling a calcium chloride solution (stationary liquid) with the concentration of 0.05mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 2;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1:1;
(1) the grouting process of (1) - (3) is a batch grouting process, and after the first batch grouting process is performed, three batches of grouting processes are performed according to the steps (1) - (3), which are respectively a second batch grouting, a third batch grouting and a fourth batch grouting, wherein the second batch grouting and the third batch grouting are completely the same as the first batch grouting, and the difference between the fourth batch grouting and the first batch grouting is that:
injecting bacterial liquid at the speed of 0.24mL/min, wherein the volume ratio of the injected bacterial liquid to the sea sand in the mold is 5;
the stationary liquid is poured in at the speed of 0.24 mL/min;
pouring the cementing liquid at the speed of 0.1 mL/min; the concentration of the cementing liquid is 0.5mol/L.
Example 3
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: domesticating Bacillus pasteurii in a culture medium with urea concentration of 20g/L and sodium chloride concentration of 10g/L for 24h, sequentially increasing the sodium chloride concentration in the culture medium to 20g/L, 25g/L, 32g/L and 35g/L, and continuing domestication until the viable count is more than 10 when the sodium chloride concentration in the culture medium is 35g/L 8 Sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L, 75g/L and 80g/L for continuous domestication until the viable count is more than 10 when the concentration of urea in the culture medium is 80g/L 8 Stopping domestication of cfu/mL;
obtaining a bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 30 ℃ for 36 h; the liquid culture medium comprises agar 20g/L, yeast extract 20g/L, ammonium sulfate 10g/L, tris 15.748g/L;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =1.0, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 2 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1), pouring a calcium chloride solution (stationary liquid) with the concentration of 0.05mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 2;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1:1;
(1) the grouting process of (3) is a batch grouting process, after the first batch grouting process is carried out, four batches of grouting processes are carried out according to the steps (1) to (3), and respectively comprise a second batch grouting, a third batch grouting, a fourth batch grouting and a fifth batch grouting, wherein the second batch grouting and the third batch grouting are completely the same as the first batch grouting, the fourth batch grouting and the fifth batch grouting are completely the same as each other, and the difference between the fourth batch grouting and the first batch grouting is that:
injecting bacterial liquid at the speed of 0.24mL/min, wherein the volume ratio of the injected bacterial liquid to the sea sand in the mold is 5;
the stationary liquid is poured in at the speed of 0.24 mL/min;
pouring the cementing liquid at the speed of 0.1 mL/min; the concentration of the cementing liquid is 0.5mol/L.
And (5) maintenance: and (3) demolding the acrylic tube after grouting to obtain sand columns, soaking the sand columns in deionized water for 36 hours, and drying the soaked sand columns at 65 ℃ for 36 hours to realize the solidification of the sea sand.
Example 4
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: domesticating Bacillus pasteurii in a culture medium with urea concentration of 20g/L and sodium chloride concentration of 10g/L for 24h, sequentially increasing the sodium chloride concentration in the culture medium to 20g/L, 25g/L, 32g/L and 35g/L, and continuing domestication until the viable count is more than 10 when the sodium chloride concentration in the culture medium is 35g/L 8 cfu/mL, sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L, 75g/L and 80g/L, and continuing to acclimatize until the viable count is more than 10 when the concentration of urea in the culture medium is 80g/L 8 Stopping domestication of cfu/mL;
obtaining a bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 30 ℃ for 36 h; the liquid culture medium comprises agar 20g/L, yeast extract 20g/L, ammonium sulfate 10g/L, tris 15.748g/L;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =1.0, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 2 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1), pouring a calcium chloride solution (stationary liquid) with the concentration of 0.05mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 2;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1:1;
(1) the grouting process of (1) - (3) is a batch grouting process, and after the first batch grouting process is performed, five batches of grouting processes are performed according to the steps (1) - (3), which are respectively a second batch grouting, a third batch grouting, a fourth batch grouting, a fifth batch grouting and a sixth batch grouting, wherein the second batch grouting and the third batch grouting are completely the same as the first batch grouting, the fourth batch grouting and the fifth batch grouting are completely the same, and the difference between the fourth batch grouting and the first batch grouting is that:
injecting bacterial liquid at the speed of 0.24mL/min, wherein the volume ratio of the injected bacterial liquid to the sea sand in the mold is 5;
the stationary liquid is poured in at the speed of 0.24 mL/min;
pouring the cementing liquid at the speed of 0.1 mL/min; the concentration of the cementing liquid is 0.5mol/L.
The difference between the sixth batch of grouting and the first batch of grouting is that:
injecting bacterial liquid at the speed of 0.24mL/min, wherein the volume ratio of the injected bacterial liquid to the sea sand in the mold is 5;
the stationary liquid is poured in at the speed of 0.24 mL/min;
pouring the cementing liquid at the speed of 0.04 mL/min; the concentration of the cementing liquid is 0.5mol/L.
And (5) maintenance: and (3) demolding the acrylic tube after grouting to obtain sand columns, soaking the sand columns in deionized water for 36 hours, and drying the soaked sand columns at 65 ℃ for 36 hours to realize the solidification of the sea sand.
Example 5
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: domesticating Bacillus pasteuri in culture medium with urea concentration of 20g/L and sodium chloride concentration of 10g/L for 24 hr, sequentially increasing sodium chloride concentration in the culture medium to 20g/L, 25g/L and 30g/L, and continuing domestication until viable count is greater than 10 when sodium chloride concentration in the culture medium is 30g/L 8 cfu/mL, sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L, 75g/L, 80g/L and 85g/L, and continuing to acclimate until the viable count is more than 10 when the concentration of urea in the culture medium is 85g/L 8 Stopping domestication of cfu/mL;
obtaining a bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 28 ℃ for 48 h; the liquid culture medium comprises 20g/L of agar, 18g/L of yeast extract, 12g/L of ammonium sulfate and 13g/L of Tris;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =0.8, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 3 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1) is finished, filling a calcium chloride solution (stationary liquid) with the concentration of 0.03mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 1;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1;
(1) the grouting process of (1) - (3) is a batch grouting process, and after the first batch grouting process is carried out, two batches of grouting processes are carried out according to the steps (1) - (3);
and (5) maintenance: and (3) demolding the acrylic tube after grouting to obtain sand columns, soaking the sand columns in deionized water for 36 hours, and drying the soaked sand columns at 65 ℃ for 36 hours to realize the solidification of the sea sand.
Example 6
The preparation method of the sea sand solidified body provided by the embodiment, which is directly applied to engineering construction as a building material, comprises the following steps:
domesticating strains: domesticating Bacillus pasteurii in a culture medium with urea concentration of 20g/L and sodium chloride concentration of 10g/L for 24h, sequentially increasing the sodium chloride concentration in the culture medium to 20g/L, 25g/L, 32g/L and 35g/L, and continuing domestication until the viable count is more than 10 when the sodium chloride concentration in the culture medium is 35g/L 8 cfu/mL, sequentially increasing the concentration of urea in the culture medium to 30g/L, 50g/L, 70g/L and 75g/L, and continuing to domesticate until the viable count is more than 10 when the concentration of urea in the culture medium is 75g/L 8 Stopping domestication of cfu/mL;
obtaining a bacterial liquid: inoculating the domesticated strain into a liquid culture medium, and culturing at 32 ℃ for 24 hours; the liquid culture medium comprises 20g/L of agar, 25g/L of yeast extract, 8g/L of ammonium sulfate and 16g/L of Tris;
filling a sand column: as shown in fig. 2, an acrylic tube with an inner diameter of 50mm is used as a mold, one end of the mold is sealed by using two layers of gauze as a filter, sea sand is filled into the mold, the filling height of the sea sand is 120mm, then the other end of the mold is sealed by using two layers of gauze as a filter, and the mold filled with the sea sand is vertically placed; sealing the bottom of the mold by using an organic glass plate with a round hole with the diameter of 3mm, and connecting a first reducer pipe at the round hole of the organic glass plate to communicate the reducer pipe into the mold; then, a rubber seal insert with a round hole with the diameter of 6mm is plugged into an opening at the upper end of the die, and a second reducer pipe is connected into the round hole of the rubber plug and communicated into the die;
grouting:
(1) diluting the obtained bacterial liquid to OD 600 =1.2, then the bacterial liquid is poured into a sand column at the speed of 0.5mL/min, and is kept stand for 2 hours; the volume ratio of the poured bacterial liquid to the sea sand in the mould is 8;
(2) after the step (1), pouring a calcium chloride solution (stationary liquid) with the concentration of 0.08mol/L into a mould at the speed of 0.5mL/min; the volume ratio of the poured fixing liquid to the sea sand in the mould is 2;
(3) after the fixing liquid is poured, pouring the cementing liquid with the concentration of 1mol/L into a mould at the speed of 0.2mL/min; the volume ratio of the poured cementing liquid to the sea sand in the mould is 50; the cementing liquid is a mixed liquid of calcium chloride and urea, wherein the molar ratio of the calcium chloride to the urea is 1:1;
(1) the grouting process of (1) - (3) is a batch grouting process, and after a first batch grouting process is performed, two batches of grouting processes are performed according to the steps (1) - (3), wherein the two batches of grouting processes are respectively a second batch grouting process and a third batch grouting process, the grouting speeds of the second batch grouting process and the first batch grouting process are completely the same, and the difference between the third batch grouting process and the first batch grouting process is as follows:
the volume ratio of the poured cementing liquid to the sea sand in the mould is 25;
and (5) maintenance: and (3) demolding the acrylic tube after grouting to obtain sand columns, soaking the sand columns in deionized water for 36 hours, and drying the soaked sand columns at 65 ℃ for 36 hours to realize the solidification of the sea sand.
Comparative example 1
This comparative example provides a method for producing a sea sand cured body, which differs from example 1 only in that: in the grouting step, the fixing liquid is not poured into each batch of grouting.
Comparative example 2
This comparative example provides a method for producing a sea sand cured body, which differs from example 1 only in that: in the grouting step, no cementing liquid is injected into each batch of grouting.
Test examples
A sand column was manufactured according to the methods of examples 1 to 7 and comparative examples 1 to 2, the manufactured sand column was cut into a standard sample having a length of 5cm, uniaxial compressive strength of the sample was measured using an electronic dynamic and static universal tester, a displacement control method was used during the test, a loading speed was 0.1mm/min, and the test results are shown in Table 1:
TABLE 1 compressive Strength test results
Test of Compressive strength (MPa)
Example 1 10.2
Example 2 20.8
Example 3 25.3
Example 4 35.6
Example 5 9.2
Example 6 10.4
Comparative example 1 3.7
Comparative example 2 6.6
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims (8)

1. A preparation method of a sea sand solidified body is characterized by comprising the following steps:
filling sea sand into the mold with openings at two ends, and sealing two ends of the mold by using a filter sheet; sequentially pouring the bacterial liquid, the stationary liquid and the cementing liquid into a mould filled with sea sand in batches, and demoulding and maintaining to obtain sand columns; filling the bacterial liquid, the stationary liquid and the cementing liquid once per cycle into one batch; co-circulating 4-6 batches of perfusion bacterial liquid, stationary liquid and cementing liquid;
the bacterial liquid comprises strains capable of producing urease, wherein the strains are strains domesticated by urea and sodium chloride; the filling speed of the bacterial liquid is 0.24-0.5mL/min; the injection speed of the stationary liquid is 0.24-0.5mL/min; the pouring speed of the cementing liquid is 0.04-0.2mL/min;
OD of the bacterial liquid 600 =0.8-1.2; the concentration of the stationary liquid is 0.03-0.08mol/L; the concentration of the cementing liquid is 0.5-1mol/L;
after the strains are domesticated, the salinity tolerance is improved to 30-35g/L, and the urea decomposition capacity is improved to 75-85g/L;
the volume ratio of the bacterial liquid filled in each batch to the sea sand in the mould is (5-8) to 12; the volume ratio of the fixing liquid filled in each batch to the sea sand in the mould is (1-2) to 12; the volume ratio of the cementing liquid filled in each batch to the sea sand in the mould is (25-50) to 12.
2. The method for preparing a solidified body of sea sand as claimed in claim 1, wherein said fixing liquid is a calcium chloride solution.
3. The method according to claim 1, wherein the cementing liquid is a mixed solution of calcium chloride and urea, and the molar ratio of the calcium chloride to the urea is 1.
4. The method according to claim 1, wherein the bacterial species is carbonate mineralization bacteria.
5. The method for preparing a sea sand solidified body according to claim 1, wherein the method for obtaining the bacterial liquid comprises:
inoculating the strain into liquid culture medium, and culturing at 28-32 deg.C for 24-48 h.
6. The method according to claim 5, wherein the liquid medium comprises yeast extract 18-25g/L, ammonium sulfate 8-12g/L, tris13-16g/L.
7. A solidified body of sea sand produced by the method for producing a solidified body of sea sand according to any one of claims 1 to 6.
8. The cured body of sea sand prepared by the method for preparing a cured body of sea sand according to any one of claims 1 to 6 is used directly in engineering construction.
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