CN109540614A - A kind of method and device preparing drift-sand mechanical test test specimen based on microorganism - Google Patents
A kind of method and device preparing drift-sand mechanical test test specimen based on microorganism Download PDFInfo
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- CN109540614A CN109540614A CN201811260696.6A CN201811260696A CN109540614A CN 109540614 A CN109540614 A CN 109540614A CN 201811260696 A CN201811260696 A CN 201811260696A CN 109540614 A CN109540614 A CN 109540614A
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- 238000012360 testing method Methods 0.000 title claims abstract description 92
- 239000004576 sand Substances 0.000 title claims abstract description 81
- 244000005700 microbiome Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 39
- 239000012530 fluid Substances 0.000 claims abstract description 29
- 238000007873 sieving Methods 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 41
- 239000007788 liquid Substances 0.000 claims description 26
- 238000002347 injection Methods 0.000 claims description 18
- 239000007924 injection Substances 0.000 claims description 18
- 239000002002 slurry Substances 0.000 claims description 11
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical group [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 239000004575 stone Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 241000193395 Sporosarcina pasteurii Species 0.000 claims description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 3
- 108010009004 proteose-peptone Proteins 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 230000000813 microbial effect Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 238000011156 evaluation Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 6
- 239000011148 porous material Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000007596 consolidation process Methods 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 230000033558 biomineral tissue development Effects 0.000 description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 239000004035 construction material Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000736262 Microbiota Species 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- NGDUODKDUZDMOI-UHFFFAOYSA-M sodium;urea;chloride Chemical compound [Na+].[Cl-].NC(N)=O NGDUODKDUZDMOI-UHFFFAOYSA-M 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of method for preparing drift-sand mechanical test test specimen based on microorganism, comprising the following steps: configuration bacterium solution；Drift-sand is sieved；It is implanted sequentially fixer, bacterium solution and consolidating fluid from bottom to top into the drift-sand after sieving, drift-sand mechanical test test specimen can be obtained.The present invention also provides a kind of devices that drift-sand mechanical test test specimen is prepared based on microorganism, prepare drift-sand mechanical test test specimen by the device.Method provided by the invention is simple and feasible, can effectively prepare drift-sand mechanical test test specimen, and can be further used for microorganism and reinforce the evaluation of drift-sand effect quantitatively；Method provided by the invention is initially injected fixer during test material preparation can provide mass efficient attachment site for microorganism, it is injected separately into bacterium solution again, consolidating fluid ensures and plays microbial activity to the maximum extent, it ensure that microorganism sufficiently reacts with surrounding chemical ingredient simultaneously, cementing effect is promoted, cementing required time is shortened.
Description
Technical field
The present invention relates to Rock And Soil strength test sample making technology fields, more particularly to one kind to prepare drift-sand based on microorganism
The method and device of mechanical test test specimen.
Background technique
Drift-sand is distributed widely in northwest China desert area, and because having, particle is tiny, intergranular is without cohesive strength, disproportionately graded
The features such as, not only it is difficult to directly provide help, or even the chief-criminal as local atmosphere polluting problem for desert area engineering construction
Chief culprit.Currently, there is larger demand for construction material in the development process of western China, transports Building wood from eastern region
Expect of a high price.It takes certain means to reinforce drift-sand, and is applied to engineering construction as architecture engineering material,
Both can alleviate problem of environmental pollution to a certain extent, at the same can be substantially improved Development of the western regions progress and save largely at
This.Therefore, drift-sand overall mechanical properties are promoted to solve the problems, such as that desert area architecture engineering material lacks, to quickening China
Construction of the West Region progress is of great significance.
It is mostly to first pass through chemical mode for its curing molding, the method that drift-sand is used at present as the research of construction material
Have many advantages, such as good forming effect, quick, but is used for a long time and can have an adverse effect to ambient enviroment.Microbial Biomineralization is cementing
It is the characteristics of can growing, migrate and breed in porous media based on microorganism, to be induced or controlled by microbiota metabolic activity
Series of chemical is in the soil body processed to improve soil mass property.Existing research shows can effective cementing wind using microbial process
Sand particle, while there are easy to operate, the advantages such as eco-compatibility is good.
Patent CN101644047 discloses a kind of method for acting on cementing loose sand grains using microbial mineralization, this method
The cementing loose sand grains by way of first injecting bacterium solution from top to bottom, injecting afenil mixed solution from bottom to top again, makes
The uniformity that the distribution of microbial mineralization product can be effectively promoted with the method, avoids the generation of injection end clogging.But this side
Method does not add urea and is difficult to sufficiently excite the deficiency of microorganism urease-producing ability when there is culture microorganism, this will be to cementing effect
Fruit has an important influence on；Meanwhile using injection bacterium solution from top to bottom, the again filling of injection afenil mixed solution from bottom to top
Slurry mode inevitably generates blocking in the middle part of sand body, this is equally unfavorable for sand body consolidation.
Summary of the invention
In view of this, the present invention provides a kind of method for preparing drift-sand mechanical test test specimen based on microorganism, it can
Effectively shorten the cementing time, drift-sand mechanical test test specimen obtained can be effective for being directed to the microorganism consolidation effect of drift-sand
Quantitative assessment additionally provides the device that a kind of structure simply prepares drift-sand mechanical test test specimen based on microorganism.
The present invention provides a kind of method for preparing drift-sand mechanical test test specimen based on microorganism, the following steps are included:
S1 configures bacterium solution；
Drift-sand is sieved by S2；
S3 is implanted sequentially fixer, bacterium solution and consolidating fluid from bottom to top into the drift-sand after sieving to get drift-sand is arrived
Mechanical test test specimen.
Further, in step S1, the bacterium solution is that strain is seeded to the bacterium cultivated in standard medium and obtained for 24 hours
Liquid.
Further, the strain is Bacillus pasteurii.
Further, the component of the standard medium includes casein peptone, soy peptone, sodium chloride, urea and goes
Ionized water.
Further, the fixer is calcium chloride solution.
Further, the consolidating fluid is the mixed solution of calcium chloride and urea.
Further, in step S3, the fixer and bacterium solution are without injection length interval, the note of the bacterium solution and consolidating fluid
4h is divided between the angle of incidence.
Further, in step S3, the fixer, bacterium solution, consolidating fluid injection number be daily injection 1 time, every time
Interval for 24 hours, is continuously injected into 1 week.
Further, in step S3, the injected slurry volume of the fixer, the injected slurry volume of bacterium solution, consolidating fluid injection body
Product is equal to the pore volume of drift-sand.
The present invention also provides a kind of device for preparing drift-sand mechanical test test specimen based on microorganism, including device for storing liquid,
Store up husky device and waste liquid tank, successively add fixer, bacterium solution and consolidating fluid in the device for storing liquid, the storage sand device from lower and
On be sequentially filled permeable stone, drift-sand and permeable stone, peristaltic pump, the wriggling are set between the device for storing liquid and Chu Sha device
One end of the pump line of pump is placed in device for storing liquid, and the other end of the peristaltic pump pump line is placed in the husky device of storage, by compacted
Fixer, bacterium solution and consolidating fluid in device for storing liquid is injected separately into the husky device of storage by dynamic pump, the upper end for storing up husky device with
Waste liquid tank connection, the waste liquid tank are collected from the solution for storing up husky device outflow.
Further, be implanted sequentially from bottom to top using above-mentioned apparatus into the drift-sand after sieving fixer, bacterium solution and
Consolidating fluid.
Technical solution provided by the invention has the benefit that
1. the method that invention provides is simple and feasible, drift-sand mechanical test test specimen can be effectively prepared, and can be further used for
Microorganism reinforces the evaluation of drift-sand effect quantitatively；
2. method provided by the invention, which is initially injected fixer during test material preparation and can provide for microorganism, largely to be had
Imitate attachment site, then be injected separately into bacterium solution, consolidating fluid ensures and plays microbial activity to the maximum extent, while ensure that micro- life
Object is sufficiently reacted with surrounding chemical ingredient, promotes cementing effect, shortens cementing required time；
3. method provided by the invention, which injects fixer, bacterium solution, consolidating fluid into drift-sand body by substep, effectively to keep away
Exempt from a large amount of calcium carbonate crystal obstruction grouting ports, while grouting quantity can be adjusted in real time according to grouting effect, saves reagent dosage；
4. apparatus structure provided by the invention is simple, easy to operate.
Detailed description of the invention
Fig. 1 is the stress-strain diagram of drift-sand mechanical test test specimen of the present invention.
Fig. 2 is the schematic device that drift-sand mechanical test test specimen and strength test use obtained by the present invention.
Fig. 3 be before microorganism of the present invention consolidation aeolian accumulation sand grain SEM (scanning electron microscope,
Scanning electron microscope) figure.
Fig. 4 is the SEM figure of the drift-sand mechanical test test specimen after present invention drying.
Fig. 5 is a kind of structural schematic diagram for the device that drift-sand mechanical test test specimen is prepared based on microorganism of the present invention.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention
Formula is further described.
The embodiment provides a kind of methods for preparing drift-sand mechanical test test specimen based on microorganism, including with
Lower step:
Bacillus pasteurii is seeded in standard medium and cultivates in 30 ± 2 DEG C, the environment of 150r/min by step S1
Grouting bacterium solution is obtained for 24 hours；The solvent of standard medium is deionized water, and the other components of standard medium are as shown in table 1:
The component of 1 standard medium of table
| Nutrient media components | Casein peptone | Soy peptone | Sodium chloride | Urea |
| Content | 15g/L | 5g/L | 5g/L | 20g/L |
Said components in table 1 are put into conical flask and are cultivated.
Drift-sand is sieved to remove the impurity in drift-sand, improves the purity of drift-sand by step S2.
Step S3 is implanted sequentially fixer, bacterium solution and consolidating fluid into the drift-sand after step S2 sieving, i.e., from bottom to top
Obtain drift-sand mechanical test test specimen.
In step S3, fixer is the calcium chloride solution that concentration is 0.01mol/L.
Consolidating fluid is the mixed solution of calcium chloride and urea, concentration 1mol/L, wherein the substance of calcium chloride and urea
The ratio between amount be 1:1.
Without injection length interval between fixer and bacterium solution, it is divided into 4h between bacterium solution and the injection length of consolidating fluid, fixer,
Bacterium solution, consolidating fluid injection number be daily injection 1 time, every minor tick for 24 hours, is continuously injected into 1 week, the injected slurry volume of fixer,
The injected slurry volume of bacterium solution, the injected slurry volume of consolidating fluid are equal to the pore volume of drift-sand, and the pore volume of drift-sand is by each
The husky amount of weighed dress is corresponding before dress is husky determines, specifically, the pore volume of drift-sand is done close according to drift-sand quality and drift-sand
Degree is calculated, and drift-sand dry density is fixed value, determines the injection body of fixer by calculating the pore volume of drift-sand
Product, the injected slurry volume of bacterium solution, consolidating fluid injected slurry volume can effectively avoid wasting.
After the completion of injection, drift-sand mechanical test test specimen top bottom surface of polishing.The test specimen polished is placed in 60 DEG C of baking ovens
To weigh twice it is of poor quality be less than 0.05g, the drift-sand mechanical test test specimen dried makes a strength test to it and SEM
(scanning electron microscope, scanning electron microscope) test.
Strength test:
The drift-sand mechanical test test specimen of drying is taken to carry out unconfined compression strength test, test gained stress-strain diagram
As shown in Figure 1, drift-sand mechanical test test specimen and strength test equipment therefor are as shown in Figure 2.As shown in Figure 1, prepared by the present invention
Drift-sand test specimen compression strength maximum up to 0.60MPa.
SEM test:
Aeolian accumulation sand grain SEM figure (x2500) before microorganism consolidation is as shown in figure 3, take the drift-sand mechanics examination after drying
It tests test specimen and carries out SEM test (x4000), test results are shown in figure 4 by SEM, and microstructure is shown, drift-sand particle surface package
A large amount of calcium carbonate crystals, it is mutually chimeric between calcium carbonate crystal, play the role of cementing aeolian accumulation sand grain, macroscopically shows as trying
Part intensity improves.
With reference to Fig. 5, the present invention also provides a kind of devices for preparing drift-sand mechanical test test specimen based on microorganism to be used for
Substep grouting test is completed, which is homemade one-dimensional provisions for grouting comprising device for storing liquid 1 stores up husky device 2 and waste liquid tank
3, device for storing liquid 1 is interior successively to add fixer, bacterium solution and consolidating fluid, storage according to the injection sequence of fixer, bacterium solution and consolidating fluid
Drift-sand 5, permeable stone 4, device for storing liquid 1 and Chu Sha device 2 after being sequentially filled permeable stone 4, sieving in husky device 2 from bottom to top
Between peristaltic pump 6 is set, one end of the pump line 61 of peristaltic pump 6 is placed in device for storing liquid 1, the pump line 61 of peristaltic pump 6 it is another
End is placed in the husky device 2 of storage, and fixer, bacterium solution and the consolidating fluid in device for storing liquid 1 are injected separately into Chu Sha by peristaltic pump 6
In device 2, fixer, bacterium solution, consolidating fluid is made to cooperate the cementing drift-sand 5 for being located at and storing up in husky device 2, is storing up the upper of husky device 2
The solution flowed out from the husky device 2 of storage is collected into waste liquid tank 3 by insertion outlet tube 21 in end by outlet tube 21；In one embodiment,
Device for storing liquid 1 and waste liquid tank 3 are beaker, store up husky device 2 and are made of organic glass, internal diameter 50mm is highly
110mm, the both ends for storing up husky device 2 are open, and fill in the first rubber stopper 7 and the second rubber stopper 8 is sealed, and are located at the husky dress of storage
Set second rubber stopper 8 aperture of 7 aperture of the first rubber stopper of 2 bottom end for connecting pump line 61, positioned at the upper end for storing up husky device 2
For connecting outlet tube 21, the rate of peristaltic pump 6 is preferably arranged to 20mL/min.
Herein, the nouns of locality such as related front, rear, top, and bottom are to be located in figure with components in attached drawing and zero
Part mutual position defines, only for the purpose of expressing the technical solution clearly and conveniently.It should be appreciated that the noun of locality
Use should not limit the claimed range of the application.
In the absence of conflict, the feature in embodiment and embodiment herein-above set forth can be combined with each other.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of method for preparing drift-sand mechanical test test specimen based on microorganism, which comprises the following steps:
S1 configures bacterium solution；
Drift-sand is sieved by S2；
S3 is implanted sequentially fixer, bacterium solution and consolidating fluid from bottom to top into the drift-sand after sieving to get drift-sand mechanics is arrived
Test test specimen.
2. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that step
In S1, the bacterium solution is that strain is seeded to the bacterium solution cultivated in standard medium and obtained for 24 hours.
3. the method for preparing drift-sand mechanical test test specimen based on microorganism as claimed in claim 2, which is characterized in that described
Strain is Bacillus pasteurii.
4. the method for preparing drift-sand mechanical test test specimen based on microorganism as claimed in claim 2, which is characterized in that described
The component of standard medium includes casein peptone, soy peptone, sodium chloride, urea and deionized water.
5. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that described
Fixer is calcium chloride solution.
6. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that described
Consolidating fluid is the mixed solution of calcium chloride and urea.
7. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that step
In S3, the fixer and bacterium solution are divided into 4h between the bacterium solution and the injection length of consolidating fluid without injection length interval.
8. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that step
In S3, the fixer, bacterium solution, consolidating fluid injection number be daily injection 1 time, every minor tick for 24 hours, is continuously injected into 1 week.
9. the method for preparing drift-sand mechanical test test specimen based on microorganism as described in claim 1, which is characterized in that step
In S3, the injected slurry volume of the fixer, the injected slurry volume of bacterium solution, consolidating fluid injected slurry volume be equal to the pore-body of drift-sand
Product.
10. a kind of device for preparing drift-sand mechanical test test specimen based on microorganism, which is characterized in that including device for storing liquid, storage
Husky device and waste liquid tank successively add fixer, bacterium solution and consolidating fluid in the device for storing liquid, and the husky device of the storage is from bottom to top
It is sequentially filled permeable stone, drift-sand and permeable stone, peristaltic pump, the peristaltic pump are set between the device for storing liquid and Chu Sha device
One end of pump line be placed in device for storing liquid, the other end of the pump line of the peristaltic pump is placed in the husky device of storage, by compacted
Fixer, bacterium solution and consolidating fluid in device for storing liquid is injected separately into the husky device of storage by dynamic pump, the upper end for storing up husky device with
Waste liquid tank connection, the waste liquid tank are collected from the solution for storing up husky device outflow.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110685266A (en) * | 2019-09-12 | 2020-01-14 | 武汉科技大学 | Method for improving mechanical property of calcareous sand by solidifying fiber reinforced microorganisms |
| CN110907328A (en) * | 2019-11-29 | 2020-03-24 | 中国海洋石油集团有限公司 | Experimental device and method for plugging and seepage-proofing rock fractures based on MICP technology |
| CN111855353A (en) * | 2020-07-28 | 2020-10-30 | 浙江科技学院 | MICP solidification calcareous sand cylinder sample preparation appearance |
| CN111912684A (en) * | 2020-08-10 | 2020-11-10 | 中国矿业大学 | Preparation system of mine microbial mineralization filling material |
| CN112198024A (en) * | 2020-09-29 | 2021-01-08 | 中国海洋大学 | Tailing sand solidification test method based on microbial grouting technology |
| CN113029816A (en) * | 2020-12-18 | 2021-06-25 | 浙江理工大学 | Real-time monitoring device and method for curing contaminated soil process based on MICP |
| CN114230253A (en) * | 2021-12-29 | 2022-03-25 | 北京建工环境修复股份有限公司 | Sea sand solidified body and preparation method and application thereof |
| CN115266300A (en) * | 2022-06-23 | 2022-11-01 | 重庆大学 | Microorganism reinforced one-dimensional compression consolidation box and test method |
| CN116081983A (en) * | 2023-01-03 | 2023-05-09 | 西京学院 | Basalt fiber reinforced aeolian sand based on MICP technology and preparation method thereof |
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| CN110907328A (en) * | 2019-11-29 | 2020-03-24 | 中国海洋石油集团有限公司 | Experimental device and method for plugging and seepage-proofing rock fractures based on MICP technology |
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| CN111912684A (en) * | 2020-08-10 | 2020-11-10 | 中国矿业大学 | Preparation system of mine microbial mineralization filling material |
| CN112198024A (en) * | 2020-09-29 | 2021-01-08 | 中国海洋大学 | Tailing sand solidification test method based on microbial grouting technology |
| CN113029816A (en) * | 2020-12-18 | 2021-06-25 | 浙江理工大学 | Real-time monitoring device and method for curing contaminated soil process based on MICP |
| CN113029816B (en) * | 2020-12-18 | 2024-05-31 | 浙江理工大学 | Real-time monitoring device and method for polluted soil curing process based on MICP |
| CN114230253A (en) * | 2021-12-29 | 2022-03-25 | 北京建工环境修复股份有限公司 | Sea sand solidified body and preparation method and application thereof |
| CN115266300A (en) * | 2022-06-23 | 2022-11-01 | 重庆大学 | Microorganism reinforced one-dimensional compression consolidation box and test method |
| CN116081983A (en) * | 2023-01-03 | 2023-05-09 | 西京学院 | Basalt fiber reinforced aeolian sand based on MICP technology and preparation method thereof |
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