CN107064472A - The experimental rig and test method of a kind of microorganism grouting and reinforcing cylinder sand sample - Google Patents
The experimental rig and test method of a kind of microorganism grouting and reinforcing cylinder sand sample Download PDFInfo
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- CN107064472A CN107064472A CN201710196531.6A CN201710196531A CN107064472A CN 107064472 A CN107064472 A CN 107064472A CN 201710196531 A CN201710196531 A CN 201710196531A CN 107064472 A CN107064472 A CN 107064472A
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Abstract
The invention discloses a kind of grouting device, including cylinder groove, Grouting Pipe, sleeve pipe, liquid outlet, the Grouting Pipe is arranged on cylinder groove center, and sleeve pipe is provided with outside Grouting Pipe, described sleeve pipe tube wall is provided with multiple apertures, and the liquid outlet is arranged on cylinder trench bottom.The invention also discloses a kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample and test method.The present invention utilizes microorganism induction precipitation of calcium carbonate（MICP）The cementing loose sand grains of technology, and in view of the stress and sand wash density in actual sand base with the increase of depth increased situation, employ the plastic bushing for having special distribution hole on a kind of wall, elevated Grouting Pipe and become flow velocity grouting mode so that the cylindrical sand sample of successively cementing formation has good uniformity and integrality along short transverse.
Description
Technical field
The invention belongs to the foundation treatment technology that the cross disciplines such as Geotechnical Engineering subject, biology and environmentology are combined
Field, is related to the experimental rig and test method of a kind of microorganism grouting and reinforcing cylinder sand sample.
Background technology
The research and application of microorganism geo-technology focus mostly on to be subtracted in the reparation of cultural relics, the burn into reduction gutter
The migration of slow heavy metal and organic pollution, prevent the piping of embankment and sill may in terms of, and consider to be used to microorganism reinforce
Sand base is then that last decade just comes into vogue, and is increasingly becoming focus.The research for reinforcing sand body on microorganism at present has some
Progress, but most of research still has the critical problems such as reinforcement depth is limited, consolidation effect is uneven, this causes micro- life
Thing reinforces the practical application of sand base and popularization receives obstruction.
The uniform curing sand sample preparation device of microorganism and its method for making sample that pin enters formula, phase are had been disclosed for before the present invention
The specimen size that it is reinforced for the present invention is smaller and device construction is complicated；A kind of microorganism cement that is used for has been also disclosed laterally to fill
The experimental rig and test method of slurry, using sand, the horizontal infiltration coefficient under stacking states is higher than its longtitudinal dispersion coefficient for it,
Reduced and blocked using laterally grouting, but aperture is also distributed with sample barrel can increase the cost of device making, and when examination
When sample is highly larger, under gravity, slurries transverse flow speed can be uneven along specimen height directional spreding, on the ground that flow velocity is slow
Side can cause the blocking of aperture in Grouting Pipe, in turn result in the uneven of sand sample entirety.
The content of the invention
Goal of the invention：The technical problems to be solved by the invention there is provided a kind of grouting device.
The technical problem of the invention also to be solved there is provided a kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample.
The technical problem of the invention also to be solved there is provided the test method of microorganism grouting and reinforcing cylinder sand sample.
Technical scheme：The present invention is achieved through the following technical solutions：A kind of grouting device, the grouting device bag
Cylinder groove, Grouting Pipe, sleeve pipe, liquid outlet are included, the Grouting Pipe is arranged on cylinder groove center, provided with set outside Grouting Pipe
Pipe, described sleeve pipe tube wall is provided with multiple apertures, and the liquid outlet is arranged on cylinder trench bottom.Pass through described sleeve pipe and cylinder groove
Between successively slip casting form cylindrical cementing sand body.
Preferably, the external diameter of described sleeve pipe is 1.2~12cm, and it is highly 0.5~10m, 1~20mm of thickness.
Preferably, the aperture on described sleeve pipe tube wall is special distribution, a diameter of 5~20mm of aperture, along pipe range
2~3cm of direction interval is arranged, arranges three kinds of upper, middle and lower of quantity point by it, every kind of aperture distributed areas respectively account for slip casting
/ 3rd of pipe height, top aperture is evenly arranged 3~4 apertures along around caliber direction, and middle part aperture is along around caliber
Direction is evenly arranged 5~6 apertures, and aperture edge in bottom is evenly arranged 7~8 apertures around caliber direction.
Preferably, described sleeve pipe is plastic bushing, its lower end is to carry cone-shaped sealing, and upper end is opening.
Preferably, the cylinder groove is made up of lucite, cylinder groove external diameter is 12~60cm, highly for 0.5~
10m, thickness is 5~10mm, and upper end is opening, and gully, liquid outlet external diameter are simulated in lower end provided with 4 cylindrical type liquid outlets
1.2~12cm, 1~20mm of thickness.
A kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample, includes described grouting device, the first peristaltic pump,
Two peristaltic pumps, microorganism slurry pool, cementing liquid pool, the first catheter, the second catheter and fixed support, the grouting device
It is located on fixed support, the first catheter is flexibly connected by peristaltic pump by microorganism slurry pool and with Grouting Pipe, second leads
Cementing liquid pool and peristaltic pump are flexibly connected by liquid pipe with Grouting Pipe.
Preferably, the grouting device is divided into first layer slip casting area, first layer slip casting area and experiment layer of sand from the bottom up.
Present invention also includes the test method of the microorganism grouting and reinforcing cylinder sand sample using described experimental rig,
Comprise the following steps：
1) first cylinder groove is vertically mounted on fixed support, in bottom, one layer of geotextiles of place mat (are no more than 200g/m2),
And it is close to one layer of glass-film, the height 2~3cm higher than sleeve pipe of glass-film on its inwall；
2) sleeve pipe is first vertically disposed in the center of cylinder groove, experiment sand is scattered between sleeve pipe and cylinder groove
(can also be layered and hit reality), makes up to required dry density (1.5~1.7g/cm3), sand wetting will be tested by then crossing water；
3) successively bacterium solution and consolidating fluid are injected by way of the first peristaltic pump and the second peristaltic pump use variable Rate；
4) during slip casting by the way of Grouting Pipe is stepped up, cylinder sand sample is successively reinforced so that cementing formation
Cylindrical sand sample there is good uniformity and integrality along short transverse.
Preferably, the strain in the bacterium solution is Bacillus pasteurii Sporosarcina pasteurii (states of the U.S.
Family strain library numbering ATCC11859).
Preferably, the consolidating fluid is CaCl2Water is dissolved in urea to obtain.
The aperture of special distribution is reserved with the tailormadepiston set tube wall of the present invention.Sleeve pipe is vertically put into cylinder groove
Center, then will experiment sand scatter between sleeve pipe and cylinder groove (can also hit it is real to a certain required dry density 1.5~
1.7g/cm3), it is allowed to be in a certain height, bacterium solution is then added and cementing into Grouting Pipe by peristaltic pump drain pipe successively
Liquid, and change during slip casting the height of Grouting Pipe, experiment sand is progressively cemented to more uniform cylindrical sand sample up and down.
Preferably, plastics Grouting Pipe is close to internal surface of sleeve pipe built in described sleeve pipe, it is possible to freely rise, thickness is 1
~10mm.
Even if the aperture on middle sleeve of the present invention is blocked, also sand can be reinforced by the layering of elevated Grouting Pipe, so that
Keep overall uniformity.
The present invention consider stress and sand wash density in actual sand base with the increase of depth increased situation, institute
Increased with the quantity of collar aperture with depth and increased, even if while sleeve pipe bottom areole is plugged, still can utilize to rise
Grouting Pipe successively reinforces sand sample to realize built in formula, reaches overall more uniform effect.
Beneficial effect：Compared with prior art, it is an advantage of the invention that：(1) it is simple in construction, it is easy to make, it is reasonable in design；
(2) stress and bulk density considered in actual sand base increases and increased situation with depth, has invented a kind of unit volume
Hole count increases and increased tailormadepiston sleeve pipe with depth, while Grouting Pipe can be risen by coordinating, is conducive to the layering reinforcing of cylinder sand sample,
Improve the overall uniformity of sand sample；(3) it is more similar to the construction gimmick of hidden pipe pile, contribute to microorganism grouting and reinforcing sand pile
Push practical application to.
Brief description of the drawings
Fig. 1 is the experimental rig structural representation of the microorganism grouting and reinforcing cylinder sand sample of the present invention；
Fig. 2 is lucite cylinder trench bottom liquid outlet schematic diagram of the invention；
Fig. 3 is built-in with the tailormadepiston sleeve pipe schematic diagram of plastics Grouting Pipe for the present invention；
Fig. 4 is grouting device structural representation of the invention；
Fig. 5 is made cylinder sand sample pictorial diagram of the invention；
Wherein, 1 it is fixed support, 2 be cylinder groove, 3 be Grouting Pipe, 4 be sleeve pipe, 5 be the first peristaltic pump, 6 is second compacted
Dynamic pump, 7 be microorganism slurry pool, 8 be cementing liquid pool, 9 be test layer of sand, 10 be liquid outlet, 11 be sleeve pipe top distribution hole,
12 it is distribution hole in the middle part of sleeve pipe, 13 be the distribution hole of casing part, 14 be second layer slip casting area, 15 is first layer slip casting area.
Embodiment
In order that technological means, character of innovation, reached purpose and the effect that the present invention is realized are of greater clarity, tie below
Conjunction is specifically illustrating, and the present invention is expanded on further.
The grouting device of embodiment 1
As shown in figure 4, the invention provides a kind of grouting device, the grouting device includes cylinder groove 2, Grouting Pipe 3, sleeve pipe
4th, liquid outlet 10, Grouting Pipe 3 is arranged on the center of cylinder groove 2, is provided with sleeve pipe 4, the tube wall of sleeve pipe 4 and sets outside Grouting Pipe 3
There are multiple apertures, liquid outlet 10 is arranged on the bottom of cylinder groove 2.The external diameter of sleeve pipe 4 is 1.2cm, is highly 52cm, thickness 1mm.Set
Aperture on the tube wall of pipe 4 is special distribution, and a diameter of 5mm of aperture is arranged along pipe range direction interval 2cm, and number is arranged by it
Three kinds of upper, middle and lower of amount point, every kind of aperture distributed areas respectively account for 1/3rd of Grouting Pipe height, and aperture edge in top is enclosed
3 apertures are evenly arranged around caliber direction, middle part aperture is evenly arranged 5 apertures along around caliber direction, and aperture edge in bottom is enclosed
7 apertures are evenly arranged around caliber direction.Sleeve pipe 4 is plastic bushing, and its lower end is to carry cone-shaped sealing, and upper end is out
Mouthful.The cylinder groove 2 is made up of lucite, and the external diameter of cylinder groove 2 is 13cm, is highly 0.5m, thickness is 5mm, upper end is
Opening, gully, liquid outlet 10 external diameter 1.2cm, thickness 1mm are simulated in lower end provided with 4 cylindrical type liquid outlets 10.
The experimental rig of the microorganism grouting and reinforcing cylinder sand sample of embodiment 2
A kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample, such as Fig. 1 includes the grouting device of embodiment 1, also wrapped
Include the first peristaltic pump 5, the second peristaltic pump 6, microorganism slurry pool 7, cementing liquid pool 8, the first catheter, the second catheter and consolidate
Fixed rack 1, grouting device is located on fixed support 1, the first catheter by peristaltic pump 5 by microorganism slurry pool 7 and with note
Slurry pipe 3 is flexibly connected, and cementing liquid pool 8 and peristaltic pump 6 are flexibly connected by the second catheter with Grouting Pipe 3.Grouting device is from lower past
On be divided into first layer slip casting area 15, first layer slip casting area 14 and experiment layer of sand 9.
The grouting device of embodiment 3
The grouting device includes cylinder groove 2, Grouting Pipe 3, sleeve pipe 4, liquid outlet 10, and Grouting Pipe 3 is arranged on the center of cylinder groove 2
Position, is provided with sleeve pipe 4 outside Grouting Pipe 3, and the tube wall of sleeve pipe 4 is provided with multiple apertures, and liquid outlet 10 is arranged on the bottom of cylinder groove 2
Portion.The external diameter of sleeve pipe 4 is 12cm, is highly 10m, thickness 20mm.Aperture on the tube wall of sleeve pipe 4 is special distribution, aperture
A diameter of 20mm, is arranged along pipe range direction interval 3cm, and three kinds of upper, middle and lower of quantity point, every kind of aperture point are arranged by it
Cloth region respectively accounts for 1/3rd of Grouting Pipe height, and aperture edge in top is evenly arranged 3 apertures, middle part aperture around caliber direction
5 apertures are evenly arranged along around caliber direction, aperture edge in bottom is evenly arranged 7 apertures around caliber direction.Sleeve pipe 4 is modeling
Expect sleeve pipe, its lower end is to carry cone-shaped sealing, and upper end is opening.The cylinder groove 2 is made up of lucite, cylinder
The external diameter of groove 2 is 60cm, is highly 10m, and thickness is 20mm, and upper end is opening, and mould is carried out in lower end provided with 4 cylindrical type liquid outlets 10
Intend gully, liquid outlet 10 external diameter 12cm, thickness 20mm.
The experimental rig of the microorganism grouting and reinforcing cylinder sand sample of embodiment 4
A kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample, includes the grouting device of embodiment 3, in addition to first
Peristaltic pump 5, the second peristaltic pump 6, microorganism slurry pool 7, cementing liquid pool 8, the first catheter, the second catheter and fixed support
1, grouting device is located on fixed support 1, and the first catheter is lived by peristaltic pump 5 by microorganism slurry pool 7 and with Grouting Pipe 3
Cementing liquid pool 8 and peristaltic pump 6 are flexibly connected by dynamic connection, the second catheter with Grouting Pipe 3.Grouting device is divided into from the bottom up
One floor slip casting area 15, first layer slip casting area 14 and experiment layer of sand 9.
The grouting device of embodiment 5
The grouting device includes cylinder groove 2, Grouting Pipe 3, sleeve pipe 4, liquid outlet 10, and Grouting Pipe 3 is arranged on the center of cylinder groove 2
Position, is provided with sleeve pipe 4 outside Grouting Pipe 3, and the tube wall of sleeve pipe 4 is provided with multiple apertures, and liquid outlet 10 is arranged on the bottom of cylinder groove 2
Portion.The external diameter of sleeve pipe 4 is 6cm, is highly 5m, thickness 10mm.Aperture on the tube wall of sleeve pipe 4 is special distribution, aperture it is straight
Footpath is 12mm, is arranged along pipe range direction interval 3cm, and three kinds of upper, middle and lower of quantity point, every kind of small pore size distribution are arranged by it
Region respectively accounts for 1/3rd of Grouting Pipe height, and aperture edge in top is evenly arranged 3 apertures, middle part aperture edge around caliber direction
5 apertures are evenly arranged around caliber direction, aperture edge in bottom is evenly arranged 7 apertures around caliber direction.Sleeve pipe 4 is plastics
Sleeve pipe, its lower end is to carry cone-shaped sealing, and upper end is opening.The cylinder groove 2 is made up of lucite, cylinder groove
2 external diameters are 36cm, are highly 5m, and thickness is 12mm, and upper end is opening, and lower end is simulated provided with 4 cylindrical type liquid outlets 10
Gully, liquid outlet 10 external diameter 6cm, thickness 10mm.
The experimental rig of the microorganism grouting and reinforcing cylinder sand sample of embodiment 6
A kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample, includes the grouting device of embodiment 5, in addition to first
Peristaltic pump 5, the second peristaltic pump 6, microorganism slurry pool 7, cementing liquid pool 8, the first catheter, the second catheter and fixed support
1, grouting device is located on fixed support 1, and the first catheter is lived by peristaltic pump 5 by microorganism slurry pool 7 and with Grouting Pipe 3
Cementing liquid pool 8 and peristaltic pump 6 are flexibly connected by dynamic connection, the second catheter with Grouting Pipe 3.Grouting device is divided into from the bottom up
One floor slip casting area 15, first layer slip casting area 14 and experiment layer of sand 9.
Embodiment 7
The experimental rig and test method of the microorganism grouting and reinforcing cylinder sand sample of embodiment 2 are real by following steps
It is existing：
Step 1: the device before slip casting is installed
1) first lucite cylinder groove 2 is vertically mounted on fixed support 1, and in cylinder groove 2 bottom place mat, one layer of soil
Work cloth (is no more than 200g/m2) prevent experiment sand from being flowed out from cylinder groove liquid outlet 10, then for convenience sand sample inside sleeve pipe 4
Take out, one layer of glass-film, the height 2~3cm higher than sleeve pipe of glass-film or so are close on the inwall of sleeve pipe 4；
2) plastic bushing 4 is first vertically disposed in the center of lucite cylinder groove 2, and experiment sand is scattered in set
Manage between cylinder groove, or reality is hit with small tool layering, make up to required density, experiment sand is highly high no more than sleeve pipe
Degree；
3) with catheter respectively by the peristaltic pump 5 of microorganism slurry pool 7 and first, the cementing peristaltic pump 6 of liquid pool 8 and second is connected
Get up, and ensure that peristaltic pump drain pipe has enough length.
4) the peristaltic pump drain pipe of microbial inoculum is first connected into built-in Grouting Pipe 3.
Step 2: configuration bacterium solution and consolidating fluid
1) bacterium solution is configured：Bacillus pasteurii Sporosarcina pasteurii (the American National strain libraries just bought
Numbering ATCC11859) peace is preserved in the vacuum drying of the state of freeze-dried powder fallen bottle, good some (enough) liquid are configured first
Culture medium, Liquid Culture based component is dusty yeast 20g/L, NH4Cl 10g/L, MnSO4·H2O 10mg/L, NiCl6H2O
24mg/L, and adjusted with 1M NaOH to pH=9.0.By fluid nutrient medium through 121 DEG C, high-temp steam sterilizing under the conditions of 30min
Afterwards, it is put into aseptic operating platform cooling stand-by, is fallen a bottle top with alcolhol burner heating peace, then drip several drip and be allowed to rupture, use tweezers
Inner tube is taken out, tampon is opened.Drawn with sterile pipette in 1mL fluid nutrient mediums injection inner tube, dissolve freeze-dried powder, will dissolve
Bacillus pasteurii Sporosarcina pasteurii afterwards are poured into the culture tube of 6mL fluid nutrient mediums, are well mixed
To bacterium solution.Then 3~4mL bacterium solutions in culture tube are seeded on 300~800mL fluid nutrient mediums, then Liquid Culture
Base places 30 DEG C, is added in microorganism slurry pool 7 after 200rpm about 30 hours of constant-temperature shaking incubator culture, and with compacted
Dynamic pump carries out slip casting.
2) consolidating fluid is configured：By CaCl2Water is dissolved in urea, 0.5M CaCl is configured to2With 1M urea mixed solution,
And be stored in standby to do in cementing liquid pool 8.
Step 3: slip casting flow
1) injection of bacterium solution：After the bacterium solution culture in step 2 is good, bacterium solution is inserted in microorganism slurry pool 7, with one
Fixed speed (10mL/min) injects about 1 times of first layer slip casting area 15 sand (high 10cm) hole by the first peristaltic pump 5 to Grouting Pipe 3
The bacterium solution of gap volume, while slowly improving 10cm Grouting Pipes 3 (in 30 seconds), makes bacterium solution flow through first layer slip casting area 15.In addition, can
To collect the bacterium solution of the outflow of sleeve pipe liquid outlet 10.And ultraviolet-uisible spectrophotometer can be utilized, test outflow bacterium solution is in wavelength
For the absorptance under 600nm spectrum, the absorptance means that bacterial concentration.It can also be created at ambient temperature with Shanghai thunder magnetic
The DDS-11A digital display conductivity meters of beneficial instrument and meter company manufacture, 9mL 1.5mol/L urea is added to by 1mL outflows bacterium solution
In, the conductivity variations within five minutes are observed, so that average minute clock conductivity variations value is obtained, by average minute clock conductance
Rate changing value is multiplied by 10 activity that can obtain bacterium solution.
2) injection of consolidating fluid：6~8 hours are stood after bacterium solution injection is finished, Grouting Pipe 3 is connected into the compacted of consolidating fluid
It is dynamic to pump out liquid pipe, consolidating fluid is injected by the second peristaltic pump 6, with the speed (9mL/min) than injecting the small 1mL/min of bacterium solution speed
Injection and the consolidating fluid of bacterium solution same volume, after 6~8 hours of interval, speed is injected and bacterium solution same volume gum deposit according to this again
Tie liquid.Furthermore it is possible to collect the consolidating fluid of the outflow of sleeve pipe liquid outlet 10.And ISO 6058-1984 can be used《Water quality calcium content
Measure EDTA titrations》Determine the calcium ion concentration in outflow consolidating fluid.HJ535-2009 can also be used《Water quality ammonia nitrogen
Determine Berthelot spectrophotometry》Determine the ammonia nitrogen concentration in outflow consolidating fluid.
3) adjustment of filling rate：One circulation is injected to the injection of bacterium solution and consolidating fluid, because every time after circulation
The sand hole gap of first layer slip casting area 15 all diminishes, therefore premised on ensureing that slurries can inject slip casting area, when following bad every time bacterium solution and
The speed of consolidating fluid will be turned down accordingly, but all keep the small 1mL/min of consolidating fluid speed ratio bacterium solution speed.
4) repeat 1), 2) He 3) step and repeatedly reinforce first layer slip casting area 15, and with slip casting hole plug, slurries are difficult to flow
Enter slip casting area for end mark.Then sand sample is successively reinforced in this approach, can obtain along the more uniform cylinder sand of height distribution
Sample, gently extracts glass-film together with sand sample during modulus.
Experimental example
By above-mentioned specific embodiment 7, Fujian standard sand compaction in layers to dry density is 1.67g/cm by the present invention3, by
Layer grouting and reinforcing produces an a diameter of 12cm, highly the cylinder sand sample for 50cm, and Fig. 5 is made cylinder sand sample pictorial diagram.
By the sand sample reinforced along the cuttings such as short transverse be 7 parts (number consecutively is A, B, C, D, E, F, G from top to bottom) carry out without side
Compression test is limited, determines and ensures that specimen surface is smooth before unconfined compressive strength, unconfined compression test, it is highly identical.Experiment
As a result it is as shown in the table.It can be seen from the table the unconfined compressive strength of sand sample be not along short transverse by increasing or by subtracting,
I.e. whole sand sample unconfined compressive strength without especially low place, be distributed along short transverse it is more uniform, this also with the present invention
Desired consolidation effect is consistent.
| Numbering | Intensity (kPa) |
| A | 1493 |
| B | 1240 |
| C | 1380 |
| D | 1390 |
| E | 1836 |
| F | 1310 |
| G | 1050 |
Claims (10)
1. a kind of grouting device, it is characterised in that the grouting device includes cylinder groove（2）, Grouting Pipe（3）, sleeve pipe（4）, go out
Liquid mouthful（10）, the Grouting Pipe（3）It is arranged on cylinder groove（2）Center, in Grouting Pipe（3）Outside is provided with sleeve pipe（4）, it is described
Sleeve pipe（4）Tube wall is provided with multiple apertures, the liquid outlet（10）It is arranged on cylinder groove（2）Bottom.
2. grouting device according to claim 1, it is characterised in that described sleeve pipe（4）External diameter be 1.2 ~ 12cm, height
For 0.5 ~ 10m, 1 ~ 20mm of thickness.
3. grouting device according to claim 1, it is characterised in that described sleeve pipe（4）Aperture on tube wall is special point
Cloth, a diameter of 5 ~ 20mm of aperture is arranged along pipe range direction 2 ~ 3cm of interval, and quantity point upper, middle and lower is arranged by it
Three kinds, every kind of aperture distributed areas respectively account for Grouting Pipe（3）/ 3rd of height, top aperture is along around the uniform cloth in caliber direction
3 ~ 4 apertures are put, middle part aperture is evenly arranged 5 ~ 6 apertures along around caliber direction, and bottom aperture is equal along caliber direction is surrounded
7 ~ 8 apertures of even arrangement.
4. grouting device according to claim 1, it is characterised in that described sleeve pipe（4）For plastic bushing, its lower end is
With cone-shaped sealing, upper end is opening.
5. grouting device according to claim 1, it is characterised in that the cylinder groove（2）It is made up of lucite, cylinder
Groove（2）External diameter is 12 ~ 60cm, is highly 0.5 ~ 10m, and thickness is 5 ~ 20mm, and upper end is opening, and lower end is provided with 4 cylindrical types
Liquid outlet（10）To simulate gully, liquid outlet（10）1.2 ~ 12cm of external diameter, 1 ~ 20mm of thickness.
6. a kind of experimental rig of microorganism grouting and reinforcing cylinder sand sample, it is characterised in that include any one of claim 1 ~ 5 institute
The grouting device stated, the first peristaltic pump（5）, the second peristaltic pump（6）, microorganism slurry pool（7）, cementing liquid pool（8）, the first drain
Pipe, the second catheter and fixed support（1）, the grouting device is located in fixed support（1）On, the first catheter passes through compacted
Dynamic pump（5）By microorganism slurry pool（7）With with Grouting Pipe（3）It is flexibly connected, the second catheter is by cementing liquid pool（8）And peristaltic pump
（6）With Grouting Pipe（3）It is flexibly connected.
7. the experimental rig of a kind of microorganism grouting and reinforcing cylinder sand sample according to claim 6, it is characterised in that described
Grouting device is divided into first layer slip casting area from the bottom up（15）, first layer slip casting area（14）With experiment layer of sand（9）.
8. the test method of the microorganism grouting and reinforcing cylinder sand sample using the experimental rig described in claim 6 or 7, its feature
It is, comprises the following steps：
1）First by cylinder groove（2）It is vertically mounted on fixed support（1）On, one layer of geotextiles of place mat in bottom, and on its inwall
It is close to one layer of glass-film, the height of glass-film compares sleeve pipe（4）High 2 ~ 3cm；
2）First by sleeve pipe（4）It is vertically disposed in cylinder groove（2）Center, will experiment sand scatter in sleeve pipe（4）And cylinder groove
（2）Between, required dry density is made up to, sand wetting will be tested by then crossing water；
3）Successively pass through the first peristaltic pump（5）With the second peristaltic pump（6）Bacterium solution and consolidating fluid are injected by the way of variable Rate；
4）Used during slip casting and step up Grouting Pipe（3）Mode, successively reinforce cylinder sand sample.
9. the test method of microorganism grouting and reinforcing cylinder sand sample according to claim 8, it is characterised in that the bacterium solution
In strain be Bacillus pasteurii.
10. the test method of microorganism grouting and reinforcing cylinder sand sample according to claim 8, it is characterised in that the glue
Knot liquid is CaCl2Water is dissolved in urea to obtain.
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| CN201710196531.6A CN107064472B (en) | 2017-03-29 | 2017-03-29 | A kind of experimental rig and test method of microorganism grouting and reinforcing cylinder sand sample |
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|---|---|---|---|
| CN201710196531.6A CN107064472B (en) | 2017-03-29 | 2017-03-29 | A kind of experimental rig and test method of microorganism grouting and reinforcing cylinder sand sample |
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| CN107064472B CN107064472B (en) | 2019-11-12 |
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| CN109629570A (en) * | 2019-01-15 | 2019-04-16 | 三峡大学 | A kind of device and method of microorganism substep consolidation by grouting sand |
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| CN110438975B (en) * | 2019-07-23 | 2021-09-17 | 河海大学 | Microbial grouting device for reinforcing deep soft clay and construction method |
| CN110438975A (en) * | 2019-07-23 | 2019-11-12 | 河海大学 | A kind of microorganism grouting device and construction method for reinforcing deep soft clay |
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| CN111672900A (en) * | 2020-06-01 | 2020-09-18 | 合肥工业大学 | Method for restoring heavy metal pollution of soil by microorganism-induced calcium carbonate precipitation |
| CN111855353A (en) * | 2020-07-28 | 2020-10-30 | 浙江科技学院 | MICP solidification calcareous sand cylinder sample preparation appearance |
| CN113155567A (en) * | 2021-04-20 | 2021-07-23 | 浙江科技学院 | Hollow cylinder sample MICP curing sample preparation instrument and sample preparation method |
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| CN113295845A (en) * | 2021-05-08 | 2021-08-24 | 广东工业大学 | Indoor simulation microorganism sand pile test method |
| CN113295846A (en) * | 2021-05-08 | 2021-08-24 | 合肥工业大学 | Test device and test method for detecting MICP (micro-emulsified asphalt) solidified polluted soil effect |
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| CN115450198A (en) * | 2022-10-31 | 2022-12-09 | 西安建筑科技大学 | Device for solidifying loess foundation through microorganism pressurized grouting and method for solidifying loess foundation |
| CN115450198B (en) * | 2022-10-31 | 2024-05-14 | 西安建筑科技大学 | Device for solidifying loess foundation through microorganism pressurization grouting and method for solidifying loess foundation |
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