CN114223892A - Microecological extract with function of reducing uric acid and preparation method thereof - Google Patents
Microecological extract with function of reducing uric acid and preparation method thereof Download PDFInfo
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- CN114223892A CN114223892A CN202111402161.XA CN202111402161A CN114223892A CN 114223892 A CN114223892 A CN 114223892A CN 202111402161 A CN202111402161 A CN 202111402161A CN 114223892 A CN114223892 A CN 114223892A
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- microecological
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- lactobacillus
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- 239000000284 extract Substances 0.000 title claims abstract description 70
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims abstract description 21
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 229940116269 uric acid Drugs 0.000 title claims abstract description 20
- 230000001603 reducing effect Effects 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title abstract description 11
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Classifications
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23V2400/00—Lactic or propionic acid bacteria
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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Abstract
The invention discloses a microecological extract with a function of reducing uric acid, which is prepared from the following components in parts by weight: 4-6 parts of apple, 12-18 parts of lemon, 4-6 parts of cherry, 2.5-3.5 parts of waxberry, 12-18 parts of pawpaw, 12-18 parts of liquorice, 0.8-1.2 parts of peach kernel powder, 3-5 parts of mint, 4-6 parts of celery, 2.5-3.5 parts of sophora flower, 3-5 parts of Chinese toon leaf, 0.8-1.2 parts of chicory, 0.8-1.2 parts of poria cocos, 0.8-1.2 parts of radish seed, 0.8-1.2 parts of sharpleaf galangal fruit, 0.8-1.2 parts of burdock root, 4-6 parts of pineapple, 12-16 parts of fructo-oligosaccharide, 0.8-1.2 parts of oat beta-glucan, 0.08-0.12 part of algin and 0.007-0.012 part of sucralose. The invention also provides a preparation method of the microecological extract. The microecological extract has the efficacy of reducing uric acid and the like.
Description
Technical Field
The invention relates to the field of health-care food, in particular to a microecological extract with a function of reducing uric acid, and also relates to a preparation method of the microecological extract.
Background
With the improvement of living standard of people, the ingestion of high protein foods such as meat, dairy products and the like is more and more, and the purine metabolism ingested by human bodies is caused to be problematic due to the lack of factors such as exercise, irregular work and rest time and the like, so that the symptoms such as overhigh uric acid in the bodies and even gout appear, and great harm is brought to the health of the human bodies. Therefore, people can treat the diseases through chemical medicines, biological medicines, Chinese herbal medicines and other medicines, but the diseases can be treated after the diseases occur, and firstly, the treatment effect is difficult to ensure; secondly, medicines such as chemical medicines can also cause certain influences on the health of human bodies, such as sequelae, adverse reactions and the like, and although the traditional Chinese herbal medicines have small side effects, the traditional Chinese herbal medicines have more impurities, are inconvenient to take and have low extraction efficiency, so that the curative effect and the application are influenced.
For this reason, microbial extraction of Chinese herbal medicines, edible animal and plant foods, has been started to improve the active ingredients and effects:
for example, the chinese patent publication No. CN107854634A discloses a fermented traditional Chinese medicine composition for treating gout and hyperuricemia, belonging to the field of traditional Chinese medicine. The fermented traditional Chinese medicine composition for treating gout and hyperuricemia comprises the following components: cordyceps militaris, radix puerariae, poria cocos, lophatherum gracile, coix seed, rhizoma drynariae, kadsura coccinea and ligusticum wallichii. The invention is based on the traditional Chinese medicine formula, combines the modern microbiology technology, and uses lactobacillus to ferment the traditional Chinese medicine to obtain the fermented medicament; the medicament has good treatment effect on hyperuricemia and gout, and has the characteristics of quick response and no toxic or side effect;
however, people find that the existing microecological extraction has some defects through research:
1) the extraction effect and efficiency are not ideal, and the existing microorganism extraction is limited by the differences of fermentation ways and metabolites of different strains, so that the selection and proportion of microorganism strains can greatly influence the extraction efficiency;
2) the absorption efficiency of the extracted product for human body is not high, and the absorption degree of the produced extract for the gastrointestinal environment of human body still has certain deficiency due to the influence of the whole fermentation environment of the existing microbial extract;
3) harmful substances exist, and the existing Chinese herbal medicines and foods inevitably contain some harmful chemical substances such as pesticide ingredients and the like, which bring certain damage to human bodies.
Therefore, there is a need for a microecological extract which has a higher extraction efficiency, a low toxic content, a high absorption by the human body, and a reduced uric acid content in the human body.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, provides a microecological extract with the function of reducing uric acid, and also provides a preparation method of the microecological extract.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microecological extract with the function of reducing uric acid is prepared from the following components in parts by weight: 4-6 parts of apple, 12-18 parts of lemon, 4-6 parts of cherry, 2.5-3.5 parts of waxberry, 12-18 parts of pawpaw, 12-18 parts of liquorice, 0.8-1.2 parts of peach kernel powder, 3-5 parts of mint, 4-6 parts of celery, 2.5-3.5 parts of sophora flower, 3-5 parts of Chinese toon leaf, 0.8-1.2 parts of chicory, 0.8-1.2 parts of poria cocos, 0.8-1.2 parts of radish seed, 0.8-1.2 parts of sharpleaf galangal fruit, 0.8-1.2 parts of burdock root, 4-6 parts of pineapple, 12-16 parts of fructo-oligosaccharide, 0.8-1.2 parts of oat beta-glucan, 0.08-0.12 part of algin and 0.007-0.012 part of sucralose.
In the invention, a further preferable scheme is that the microecological extract is prepared from the following components in parts by weight: 5 parts of apple, 15 parts of lemon, 5 parts of cherry, 3 parts of waxberry, 15 parts of pawpaw, 15 parts of liquorice, 1 part of peach kernel powder, 4 parts of mint, 5 parts of celery, 3 parts of sophora flower, 4 parts of Chinese toon leaf, 1 part of chicory, 1 part of poria cocos, 1 part of radish seed, 1 part of sharpleaf galangal fruit, 1 part of burdock root, 5 parts of pineapple, 14 parts of fructo-oligosaccharide, 0.89 part of oat beta-glucan, 0.1 part of algin and 0.01 part of sucralose.
The invention also provides a preparation method of the microecological extract, which comprises the following steps:
step 1: taking the components according to a formula, mixing, sterilizing, and performing enzymolysis to obtain an enzymolysis raw material mixture;
step 2: carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microbes, then filtering the mixture obtained after the microbial ecological extraction, removing filter residues, and collecting a microbial ecological extracting solution;
and step 3: and (3) sterilizing the microecological extracting solution obtained in the step (2), and then curing to obtain the microecological extract.
In the present invention, it is further preferable that the method further comprises, after the step 3, a step 4: adding food acceptable auxiliary materials into the microecological extract obtained in the step 3.
In the present invention, it is further preferable that step 4 is followed by step 5: heating the microecological extract treated in the step 4 until the auxiliary materials are dissolved, and then sterilizing; the adjuvant is soluble in water.
In the present invention, it is further preferable that the enzymatic hydrolysis is performed using a C1 enzyme, a Cx enzyme, and a protease.
In the invention, a further preferable scheme is that the extraction temperature in the step 2 is 35-39 ℃, and the extraction time is 2-4 years.
In the present invention, it is further preferable that the aging treatment in the step 3 specifically comprises: and (3) placing the microecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature.
In the present invention, it is further preferred that the microorganism in step 2 comprises the following strains in percentage by weight of the total amount of each component (the percentage by weight herein refers to the ratio of the amount of the strains to the total weight of each component of the microecological extract x 100%): lactobacillus acidophilus 0.0133%, lactobacillus casei 0.0133%, lactobacillus rhamnosus 0.0133%, lactobacillus gasseri 0.01%, lactobacillus johnsonii 0.01%, lactobacillus salivarius 0.01%, bifidobacterium breve 0.015%, and bifidobacterium infantis 0.015%.
In the present invention, it is further preferable that the microorganism in step 2 further includes a microorganism for degrading an organophosphorus pesticide.
Compared with the prior art, the invention has the beneficial effects that the microecological extract has the following effects by screening and proportioning optimization of fermentation microbial strains:
1) the probiotic composition has high content of active ingredients, and can secrete protease, cellulase, hemicellulase, pectinase, amylase and other extracellular enzymes in the process of growing, reproducing and metabolizing by using each component as a nutrient substance, so that cells of the traditional Chinese medicine are cracked, the active ingredients of each component can be released from the cells, and the active ingredients in each component can be extracted and utilized to the maximum extent; the obtained microecological extract has uric acid reducing effect;
2) the beneficial component content is high, and the probiotic composition can generate substances such as polysaccharide beneficial to a human body in the secondary metabolism process, and finally enter the extracting solution to improve the content of the beneficial substances in the extracting solution;
3) the probiotic composition disclosed by the invention has high human body absorptivity, macromolecular substances which cannot be absorbed by a human body are degraded into active micromolecules which can be absorbed by the human body, and the micromolecular substances can be combined with cell proteins of an organism through a blood brain barrier, so that the absorption and utilization of the organism to effective components are improved;
4) the probiotics extracting method has the advantages that the variety of active ingredients is various, in the process of extracting the probiotics through combination, part of the ingredients in each component have inhibition/promotion effects on the growth and metabolism of microorganisms and the generation of the active ingredients, and the microorganisms can change own metabolic pathways in the special environment of each component, so that richer primary or secondary metabolites are generated, and more active ingredients are formed;
5) the probiotic composition disclosed by the invention can degrade toxic components in the traditional Chinese medicine components, such as alkaloids, glycosides, lactones, protein heavy metals and the like, or structurally modify toxic active ingredients of the traditional Chinese medicine, so that the toxic and side effects are reduced;
6) active ingredients are prevented from being damaged, and the damage to the active ingredients in the traditional Chinese medicine decocting, boiling, steaming, refining and other processes is avoided by adopting a microecological extraction process;
in addition, the preparation method is easy to operate and convenient for industrial production.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment. Except as specifically noted, the materials and equipment used in this example are commercially available. The specific embodiments are merely illustrative and are not to be construed as limiting the scope of the invention.
A microecological extract with the function of reducing uric acid is prepared from the following components in parts by weight: 4-6 parts of apple, 12-18 parts of lemon, 4-6 parts of cherry, 2.5-3.5 parts of waxberry, 12-18 parts of pawpaw, 12-18 parts of liquorice, 0.8-1.2 parts of peach kernel powder, 3-5 parts of mint, 4-6 parts of celery, 2.5-3.5 parts of sophora flower, 3-5 parts of Chinese toon leaf, 0.8-1.2 parts of chicory, 0.8-1.2 parts of poria cocos, 0.8-1.2 parts of radish seed, 0.8-1.2 parts of sharpleaf galangal fruit, 0.8-1.2 parts of burdock root, 4-6 parts of pineapple, 12-16 parts of fructo-oligosaccharide, 0.8-1.2 parts of oat beta-glucan, 0.08-0.12 part of algin and 0.007-0.012 part of sucralose.
Through the screening and the proportioning optimization of the fermentation microbial strains, the microecological extract has the following effects:
1) the probiotic composition has high content of active ingredients, and can secrete protease, cellulase, hemicellulase, pectinase, amylase and other extracellular enzymes in the process of growing, reproducing and metabolizing by using each component as a nutrient substance, so that cells of the traditional Chinese medicine are cracked, the active ingredients of each component can be released from the cells, and the active ingredients in each component can be extracted and utilized to the maximum extent; the obtained microecological extract has uric acid reducing effect;
2) the beneficial component content is high, and the probiotic composition can generate substances such as polysaccharide beneficial to a human body in the secondary metabolism process, and finally enter the extracting solution to improve the content of the beneficial substances in the extracting solution;
3) the probiotic composition disclosed by the invention has high human body absorptivity, macromolecular substances which cannot be absorbed by a human body are degraded into active micromolecules which can be absorbed by the human body, and the micromolecular substances can be combined with cell proteins of an organism through a blood brain barrier, so that the absorption and utilization of the organism to effective components are improved;
4) the probiotics extracting method has the advantages that the variety of active ingredients is various, in the process of extracting the probiotics through combination, part of the ingredients in each component have inhibition/promotion effects on the growth and metabolism of microorganisms and the generation of the active ingredients, and the microorganisms can change own metabolic pathways in the special environment of each component, so that richer primary or secondary metabolites are generated, and more active ingredients are formed;
5) the probiotic composition disclosed by the invention can degrade toxic components in the traditional Chinese medicine components, such as alkaloids, glycosides, lactones, protein heavy metals and the like, or structurally modify toxic active ingredients of the traditional Chinese medicine, so that the toxic and side effects are reduced;
6) avoids the damage of active ingredients, adopts a microecological extraction process, and avoids the damage of the active ingredients in the traditional Chinese medicine processes of decocting, boiling, steaming, refining and the like.
The preparation method of the microecological extract comprises the following steps:
step 1: taking the components according to the formula, mixing, sterilizing, and performing enzymolysis to obtain an enzymolysis raw material mixture
Step 2: carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microbes, then filtering the mixture obtained after the microbial ecological extraction, removing filter residues, and collecting a microbial ecological extracting solution;
and step 3: and (3) sterilizing the microecological extracting solution obtained in the step (2), and then curing to obtain the microecological extract.
In order to be more convenient for consumers to eat, auxiliary materials acceptable for food can be added into the microecological extract obtained in the step 3 to prepare different product dosage forms so as to enrich the mouthfeel of the product and facilitate storage and transportation, and specifically, a sweetening agent and the like can be added according to the needs or the preference of consumers, or the microecological extract is further prepared into oral liquid, tablets and the like; for example, step 4 is followed by step 5: heating the microecological extract treated in the step 4 until the auxiliary materials are dissolved, and then sterilizing; the adjuvants are soluble in water, and can be sweetener (such as granulated sugar, sucralose, mogroside), etc.
In order to improve the quality of the microecological extract, the fermentation temperature in the step 2 is 35-39 ℃, and the fermentation time is 2-4 years.
For enzymolysis, in order to facilitate subsequent microecological treatment, the enzymolysis is carried out by using C1 enzyme, Cx enzyme and protease; wherein, the C1 enzyme destroys the crystal structure of the cellulose chain, the Cx enzyme acts on the cellulose activated by the C1 enzyme to decompose the cellulose with beta-1, 4-glycosidic bond, the protease breaks the peptide bond of the connecting amino acid, the three enzymes cooperate to decompose 99 percent of the substances in the raw materials into micromolecules, thus the microbial fermentation in the follow-up micro-ecology is more convenient, and the effect is better.
The sterilization treatment is not particularly limited, and for the sake of facilitating industrial operation, ozone sterilization treatment may be employed for 4 to 8 min.
For filtering the microorganism extract, a 100-mesh sieve can be selected for filtering; for the sterilization treatment of the microorganism extract in step 3, pasteurization can be carried out, such as sterilization at 85 + -5 deg.C for 30-35 min; after the microbial extracting solution is treated in the steps 4 and 5, the treatment such as sterilization, filling, packaging and the like can be continuously carried out for facilitating the storage and transportation of the product; in order to inspect and monitor the quality of the product, the physical and chemical indexes of the product, such as total acid, crude polysaccharide, free amino acid, short-chain fatty acid, microorganism and the like, can be detected.
In order to enhance the flavor of the microbial extract of the present invention, a curing treatment may be performed, and the curing treatment in step 3 is specifically: placing the micro-ecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature; by aging, fructo-oligosaccharide in the microorganism extractive solution can be aged to generate aromatic hydrocarbon, which can improve flavor and aromatic hydrocarbon effect.
In order to improve the active ingredient content and the human body availability of the microbial extract and reduce the content of harmful substances, the microbes in the step 2 comprise the following strains in percentage by weight according to the total amount of the components: lactobacillus acidophilus 0.0133%, lactobacillus casei 0.0133%, lactobacillus rhamnosus 0.0133%, lactobacillus gasseri 0.01%, lactobacillus johnsonii 0.01%, lactobacillus salivarius 0.01%, bifidobacterium breve 0.015%, and bifidobacterium infantis 0.015%.
In order to further reduce the content of harmful substances in the microecological extract of the present invention, the microorganisms in step 2 further include microorganisms for degrading organophosphorus pesticides; as the microorganism for degrading the organophosphorus pesticide, bacteria, fungi and algae can be selected, and correspondingly such as:
chlorpyrifos is degraded, and the following bacteria can be selected: bacillus subtilis inaquosorium strain KCTC13429, Bacillus cereus ATCC 14579Bacillus safensis F0-36b, Sphingomonasp, Pseudomonas nitroreducens PS-2, Pseudomonas fluorescens, Brucella melitensis, Bacillus subtilis, Bacillus cereus, Klebsiella sp, Serratia sp, Pseudomonas aeruginosa, Pseudomonas sp, Agrobacterium sp, Bacillus pumilus C2A1, the following fungi can be selected: aspergillus terreus, Trichoderma harzianum, Penicillium brevicompactum, Trichoderma sp, optionally algae Synechocystis PUPCCC 64;
the phoxim can be degraded by the following bacteria: plouomonas sp.X-1, Delftia sp.XSP-1, Bacillus subtilis Bs-15, Stenotrophoromonas sp.G1, Ochrobactrum sp.DDV-1; the following fungi may be selected: paecilomyces larlicinus NH-PL-03, Trichoderma atroviride;
degrading trichlorfon by using Rhodobacter sphaeroides, and degrading triazophos by using Stenotrophormonas sp.G1 and Bacillus sp.TAP-1;
specifically, in the microecological extraction process of the present invention, the above-listed microorganisms may be selected based on the need, one or a combination of two or more of them may be selected, or other microorganisms not listed may be selected based on the need to be combined with the above-listed microorganisms.
Example 1
A microecological extract with the function of reducing uric acid is prepared from the following components in parts by weight: 5 parts of apple, 15 parts of lemon, 5 parts of cherry, 3 parts of waxberry, 15 parts of pawpaw, 15 parts of liquorice, 1 part of peach kernel powder, 4 parts of mint, 5 parts of celery, 3 parts of sophora flower, 4 parts of Chinese toon leaf, 1 part of chicory, 1 part of poria cocos, 1 part of radish seed, 1 part of sharpleaf galangal fruit, 1 part of burdock root, 5 parts of pineapple, 14 parts of fructo-oligosaccharide, 0.89 part of oat beta-glucan, 0.1 part of algin and 0.01 part of sucralose;
the preparation method of the ferment comprises the following steps:
step 1: taking the components according to a formula, mixing, sterilizing, and performing enzymolysis to obtain an enzymolysis raw material mixture; c1 enzyme, Cx enzyme and protease are selected for enzymolysis;
step 2: carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 for 150 days by using microbes, then filtering (sieving by a 100-mesh sieve) the mixture obtained after the microbial ecological extraction, removing filter residues, and collecting a microbial ecological extracting solution;
and step 3: sterilizing the microecological extracting solution obtained in the step 2, and then curing to obtain a microecological extract; the curing treatment in the step 3 specifically comprises the following steps: placing the micro-ecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature; obtaining the micro-ecological extract;
the microorganism in the step 2 comprises the following strains in percentage by weight of the total amount of the components: lactobacillus acidophilus 0.0133%, lactobacillus casei 0.0133%, lactobacillus rhamnosus 0.0133%, lactobacillus gasseri 0.01%, lactobacillus johnsonii 0.01%, lactobacillus salivarius 0.01%, bifidobacterium breve 0.015%, and bifidobacterium infantis 0.015%.
Example 2
A microecological extract with the function of reducing uric acid is prepared from the following components in parts by weight: 6 parts of apple, 18 parts of lemon, 4 parts of cherry, 2.5 parts of waxberry, 12 parts of pawpaw, 18 parts of liquorice, 0.8 part of peach kernel powder, 5 parts of mint, 4 parts of celery, 2.5 parts of sophora flower, 3 parts of Chinese toon leaf, 1.2 parts of chicory, 0.8 part of tuckahoe, 1.2 parts of radish seed, 0.8 part of sharpleaf galangal fruit, 0.8 part of burdock root, 4 parts of pineapple, 16 parts of fructo-oligosaccharide, 1.2 parts of oat beta-glucan, 0.08 part of algin and 0.012 part of sucralose;
the microbial strains and the proportion in the step 2 are the same as those in the example 1; the preparation method is the same as that of example 1.
Example 3
A microecological extract with the function of reducing uric acid is prepared from the following components in parts by weight: 4 parts of apple, 12 parts of lemon, 6 parts of cherry, 3.5 parts of waxberry, 18 parts of pawpaw, 12 parts of liquorice, 1.2 parts of peach kernel powder, 3 parts of mint, 6 parts of celery, 3.5 parts of sophora flower, 5 parts of Chinese toon leaf, 0.8 part of chicory, 1.2 parts of tuckahoe, 0.8 part of radish seed, 1.2 parts of sharpleaf galangal fruit, 1.2 parts of burdock root, 6 parts of pineapple, 12 parts of fructo-oligosaccharide, 0.8 part of oat beta-glucan, 0.12 part of algin, 0.007 part of sucralose
The microbial strains and the proportion in the step 2 are the same as those in the example 1; the preparation method is the same as that of example 1.
Experimental example 1
Taking the microecological extract with the function of reducing uric acid in example 1, and then detecting the contents of polyphenol, flavone, saponin, amino acid and harmful substances in the microecological extract, wherein the specific detection results are shown in the following tables 1-3:
TABLE 1 active ingredient content test Table in microecological extracts
As can be seen from the data in table 1 above: the microecological extract with the function of reducing uric acid has high contents of polyphenol, total flavone, proanthocyanidin, catechin and the like, and the obtained extract has the function of reducing uric acid.
TABLE 2 test chart of amino acid content in microecological extract
As can be seen from the data in table 2 above: the microecological extract with the function of reducing uric acid has complete types and high content of essential amino acids for human bodies.
TABLE 3 content test Table for part of colonies, lead and saccharin sodium in the microecological extract
From the detection data in table 3 above, it can be seen that each harmful bacterium and saccharin sodium in the microecological extract of the present invention all meet the national standards, and the microecological extract of the present invention has high safety.
Comparative example 1
Taking the components according to the microecological components and the proportion thereof in the embodiment 1, mixing, sterilizing, and then carrying out superfine grinding to obtain a ground raw material mixture; and adding water, decocting for 3 times, decocting for 30min each time, collecting the filtrate obtained by 3 times of decoction through filtration, and combining the filtrates to obtain the filtrate of comparative example 1 (namely the extract of comparative example 1).
Respectively taking 2 parts of the same weight of the microecological formulation according to the example 1, then respectively adopting the methods of the example 1 and the comparative example 1 to obtain the microecological extract of the example 1 and the extract of the comparative example 1, concentrating the 2 parts of the extract to the same weight (concentrating the extract of the comparative example 1 to the same weight based on the microecological extract of the example 1), then respectively testing the content of each nutrient component and the total amount of hydrolyzed amino acid in the extract, wherein the test result data are shown in the following table:
table 4: data table for active nutrient ingredient content test of example 1 and comparative example 1
As can be seen from the data in Table 4, compared with the traditional boiling process of comparative example 1, the microecological extraction of the invention can better release active nutritional ingredients, so that the microecological extract of the invention has higher nutritional active ingredients and better promotion and improvement effects on reducing the uric acid function of a human body.
Finally, it should be noted that: the above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention should not be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (10)
1. A microecological extract with the function of reducing uric acid is characterized by being prepared from the following components in parts by weight: 4-6 parts of apple, 12-18 parts of lemon, 4-6 parts of cherry, 2.5-3.5 parts of waxberry, 12-18 parts of pawpaw, 12-18 parts of liquorice, 0.8-1.2 parts of peach kernel powder, 3-5 parts of mint, 4-6 parts of celery, 2.5-3.5 parts of sophora flower, 3-5 parts of Chinese toon leaf, 0.8-1.2 parts of chicory, 0.8-1.2 parts of poria cocos, 0.8-1.2 parts of radish seed, 0.8-1.2 parts of sharpleaf galangal fruit, 0.8-1.2 parts of burdock root, 4-6 parts of pineapple, 12-16 parts of fructo-oligosaccharide, 0.8-1.2 parts of oat beta-glucan, 0.08-0.12 part of algin and 0.007-0.012 part of sucralose.
2. The microecological extract according to claim 1, which is prepared from the following components in parts by weight: 5 parts of apple, 15 parts of lemon, 5 parts of cherry, 3 parts of waxberry, 15 parts of pawpaw, 15 parts of liquorice, 1 part of peach kernel powder, 4 parts of mint, 5 parts of celery, 3 parts of sophora flower, 4 parts of Chinese toon leaf, 1 part of chicory, 1 part of poria cocos, 1 part of radish seed, 1 part of sharpleaf galangal fruit, 1 part of burdock root, 5 parts of pineapple, 14 parts of fructo-oligosaccharide, 0.89 part of oat beta-glucan, 0.1 part of algin and 0.01 part of sucralose.
3. A method for preparing the microecological extract according to claim 1, characterized in that it comprises the steps of:
step 1: taking the components according to a formula, mixing, sterilizing, and performing enzymolysis to obtain an enzymolysis raw material mixture;
step 2: carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microbes, then filtering the mixture obtained after the microbial ecological extraction, removing filter residues, and collecting a microbial ecological extracting solution;
and step 3: and (3) sterilizing the microecological extracting solution obtained in the step (2), and then curing to obtain the microecological extract.
4. The method for preparing a microecological extract according to claim 3, further comprising the step 4 after the step 3: adding food acceptable auxiliary materials into the microecological extract obtained in the step 3.
5. The method for preparing a microecological extract according to claim 4, further comprising the step 5 after the step 4: heating the microecological extract treated in the step 4 until the auxiliary materials are dissolved, and then sterilizing; the adjuvant is soluble in water.
6. The method of claim 3, wherein the enzymatic hydrolysis is performed using C1 enzyme, Cx enzyme, and protease.
7. The method for preparing a microecological extract according to claim 3, wherein: the extraction temperature in the step 2 is 35-39 ℃, and the extraction time is 2-4 years.
8. The method for preparing a microecological extract according to claim 3, wherein the aging treatment in the step 3 is specifically: and (3) placing the microecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature.
9. The method for preparing a microecological extract according to claim 3, wherein the microorganisms of step 2 comprise the following species in percentage by weight based on the total amount of each component: lactobacillus acidophilus 0.0133%, lactobacillus casei 0.0133%, lactobacillus rhamnosus 0.0133%, lactobacillus gasseri 0.01%, lactobacillus johnsonii 0.01%, lactobacillus salivarius 0.01%, bifidobacterium breve 0.015%, and bifidobacterium infantis 0.015%.
10. The method for preparing a microecological extract according to claim 3, wherein the microorganisms of step 2 further comprise microorganisms for degrading organophosphorus pesticides.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20130330299A1 (en) * | 2002-03-13 | 2013-12-12 | Kibow Biotech, Inc. | Composition and method for preventing or treating gout or hyperuricemia |
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| CN109172770A (en) * | 2018-11-13 | 2019-01-11 | 张洪搏 | Facilitate the herbal composite and preparation method thereof of uric acid adjusting |
| CN109224007A (en) * | 2018-11-14 | 2019-01-18 | 厦门元之道生物科技有限公司 | A kind of fermented tcm agent and preparation method thereof with auxiliary anti-trioxypurine |
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2021
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| US20130330299A1 (en) * | 2002-03-13 | 2013-12-12 | Kibow Biotech, Inc. | Composition and method for preventing or treating gout or hyperuricemia |
| CN105996002A (en) * | 2016-06-08 | 2016-10-12 | 江西百禾生物科技有限公司 | Plant fermentation enzyme powder and making technology |
| CN109172770A (en) * | 2018-11-13 | 2019-01-11 | 张洪搏 | Facilitate the herbal composite and preparation method thereof of uric acid adjusting |
| CN109224007A (en) * | 2018-11-14 | 2019-01-18 | 厦门元之道生物科技有限公司 | A kind of fermented tcm agent and preparation method thereof with auxiliary anti-trioxypurine |
| CN111248382A (en) * | 2020-02-28 | 2020-06-09 | 山东理工大学 | Gardenia and poria cocos compound beverage and preparation method thereof |
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