CN114215395A - Animal isolated cell laboratory - Google Patents
Animal isolated cell laboratory Download PDFInfo
- Publication number
- CN114215395A CN114215395A CN202111526159.3A CN202111526159A CN114215395A CN 114215395 A CN114215395 A CN 114215395A CN 202111526159 A CN202111526159 A CN 202111526159A CN 114215395 A CN114215395 A CN 114215395A
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- Prior art keywords
- room
- corridor
- air outlet
- inlet
- animal
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- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
- E04H1/00—Buildings or groups of buildings for dwelling or office purposes; General layout, e.g. modular co-ordination or staggered storeys
- E04H1/12—Small buildings or other erections for limited occupation, erected in the open air or arranged in buildings, e.g. kiosks, waiting shelters for bus stops or for filling stations, roofs for railway platforms, watchmen's huts or dressing cubicles
- E04H1/1277—Shelters for decontamination
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F3/00—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems
- F24F3/12—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling
- F24F3/16—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling by purification, e.g. by filtering; by sterilisation; by ozonisation
- F24F3/167—Clean rooms, i.e. enclosed spaces in which a uniform flow of filtered air is distributed
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F7/00—Ventilation
- F24F7/003—Ventilation in combination with air cleaning
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F7/00—Ventilation
- F24F7/04—Ventilation with ducting systems, e.g. by double walls; with natural circulation
- F24F7/06—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit
- F24F7/08—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit with separate ducts for supplied and exhausted air with provisions for reversal of the input and output systems
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
- F24F8/10—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering
- F24F8/108—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering using dry filter elements
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
- F24F8/20—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation
- F24F8/22—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation using UV light
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Combustion & Propulsion (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Architecture (AREA)
- Civil Engineering (AREA)
- Structural Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an animal in-vitro cell laboratory, which relates to the technical field of biological laboratories and comprises a changing room, a preparation room and a pedestrian corridor, wherein the changing room is provided with a first inlet, the preparation room is communicated with the changing room through a second inlet, the pedestrian corridor is arranged perpendicular to the preparation room and is communicated with the middle part of the preparation room through a third inlet, two sides of the pedestrian corridor are respectively provided with an operation room, one end of the pedestrian corridor far away from the preparation room is provided with two fourth inlets, the fourth inlets are used for communicating the pedestrian corridor with the operation room, and the first inlet, the second inlet, the third inlet and the fourth inlets are respectively provided with a first sealing door. The animal in vitro cell laboratory structure dynamic line is reasonable in design, can effectively keep the interior of the laboratory clean, and can greatly improve the efficiency of animal in vitro cell culture.
Description
Technical Field
The invention belongs to the technical field of biological laboratories, and particularly relates to an animal in-vitro cell laboratory.
Background
Animal ex vivo cell culture (animal cell culture) is a process in which the relevant tissue is removed from the animal body, dispersed into individual cells (using trypsin or collagenase), and then placed in a suitable medium to allow these cells to grow and proliferate. The main difference between tissue cell culture techniques and other common laboratory work is the requirement to maintain sterile operation, avoiding the effects of microorganisms and other harmful factors.
At present, the design structure of a commonly used animal cell in-vitro culture laboratory is complex, the moving line design is unreasonable, and the requirement of animal cell in-vitro culture cannot be met.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide an isolated cell laboratory which has a reasonable structure moving line design and can ensure the culture efficiency of isolated cells of animals.
In order to achieve the technical effect, the invention adopts the following technical scheme:
animal separation cell laboratory, including dressing room, preparation room and people's line corridor, be equipped with first entry on the dressing room, prepare room through the second entry with dressing room intercommunication, people's line corridor perpendicular to set up between the preparation and through the third entry with middle part intercommunication between the preparation, people's line corridor's both sides respectively are provided with an operation room, people's line corridor is keeping away from one end between the preparation is equipped with two fourth entries, the fourth entry is used for the intercommunication people's line corridor and operation room, first entry, second entry, third entry and fourth entry all are equipped with a first sealing door.
Furthermore, a linear material channel is arranged between each preparation room and each operation room, two ends of each material channel are provided with a second sealing door, and material conveying equipment is arranged in each material channel.
Further, material conveying equipment including slidable mounting in the material shelf in the material passageway, be equipped with the material shallow on the material shelf, still be equipped with in the material passageway and be used for driving the material shelf is twice the linear driving piece that the second closed door removed between.
Further, still include the malleation blast pipe, be equipped with first air outlet, second air outlet, third air outlet, fourth air outlet and fifth air outlet in dressing room, the room of preparing, pedestrian's corridor, operation room and the material passageway respectively, the air-out end of malleation blast pipe respectively through first tuber pipe, second tuber pipe, third tuber pipe, fourth tuber pipe and fifth tuber pipe with first air outlet, second air outlet, third air outlet, fourth air outlet and fifth air outlet intercommunication.
Furthermore, the air inlet end of the positive pressure air supply pipe is at least provided with a group of particle filtering components, and the downstream of the particle filtering components is provided with an ultraviolet sterilization component.
Further, the wall body bottom of dressing room, preparation room, pedestrian corridor, operation room and material passageway all is equipped with the air exit, the air exit passes through exhaust duct and external environment intercommunication, exhaust duct is pre-buried in the wall body just be equipped with the exhaust fan in the exhaust duct.
Further, the air exit in the people's line corridor is located people's line corridor is close to the one end between preparing and is located the wall body bottom, the third air outlet is located people's line corridor is kept away from one end between preparing and is located people's line corridor's top.
The invention has the beneficial effects that: according to the isolated animal cell laboratory provided by the invention, the dressing room, the preparation room, the pedestrian corridor and the operation room are arranged, and the dressing room, the preparation room, the pedestrian corridor and the operation room are reasonably distributed, so that the isolated animal cell laboratory is compact in structure and reasonable in dynamic line, can meet the requirement of isolated animal cell culture, and improves the efficiency of isolated animal cell culture. In addition, the material passageway through setting up and the separation of pedestrian corridor is used for carrying out the automation to the material for get into the operating personnel and the material of operating room and shunt, further reduced the difficulty of killing of this animal isolated cell laboratory, reduced the pollution probability of this operating room.
Drawings
FIG. 1 is a schematic diagram of the overall structure of an animal in vitro cell laboratory according to an embodiment of the present invention;
FIG. 2 is a schematic plan view of an isolated animal cell laboratory according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a linear driving member of an animal ex-vivo cell laboratory according to an embodiment of the invention.
The notation in the figure is: 10-positive pressure blast pipe, 20-dressing room, 21-first inlet, 22-first air pipe, 30-preparation room, 31-second inlet, 32-second air pipe, 40-pedestrian corridor, 41-third inlet, 42-fourth inlet, 43-third air pipe, 50-operation room, 51-preparation room, 52-cell room, 53-fourth air pipe, 60-material channel, 61-fifth air pipe, 70-exhaust duct, 81-material shelf, 82-material cart, 83-driving wheel, 84-driven wheel, 85-synchronous belt, 86-positive and negative rotation motor and 87-guide rail.
Detailed Description
In order to facilitate understanding of those skilled in the art, the present invention will be further described with reference to the following examples and drawings, which are not intended to limit the present invention.
As shown in fig. 1 to 3, the laboratory for in vitro animal cells provided in this embodiment includes a dressing room 20, a preparation room 30, a pedestrian corridor 40, and an operation room 50, wherein the dressing room 20 is provided with a first entrance 21. The preparation room 30 is communicated with the dressing room 20 through the second inlet 31, the people corridor 40 is perpendicular to the preparation room 30 and is communicated with the middle of the preparation room 30 through the third inlet 41, the operation room 50 is provided with two inlets which are respectively positioned at two sides of the people corridor 40, one end of the people corridor 40 far away from the preparation room 30 is provided with two fourth inlets 42, the fourth inlets 42 are used for communicating the people corridor 40 with the operation room 50, and the first inlet 21, the second inlet 31, the third inlet 41 and the fourth inlets 42 are all provided with a first sealing door. In order to convey materials, equipment and the like conveniently, a linear material channel 60 is arranged between the preparation room 30 and each operation room 50, two ends of the material channel 60 are provided with a second closing door, and the material channel 60 is internally provided with material conveying equipment. In the animal in-vitro cell laboratory, the route for an operator to enter the operating room 50 is the changing room 20-the preparation room 30-the pedestrian corridor 40-the operating room 50, and the route for a material to enter the operating room 50 is the changing room 20-the preparation room 30-the material channel 60-the operating room 50, so that the animal in-vitro cell laboratory can shunt the operator and the material, the sterilization difficulty can be reduced, and the cleanliness of the operating room 50 can be improved.
In this embodiment, to facilitate the material transportation, the material transportation device includes a material shelf 81 slidably mounted in the material passage 60, a material cart 82 is disposed on the material shelf 81, and the material cart 82 is preferably a multi-layer cart for placing materials, instruments or devices. A linear drive for driving the material shelf 81 between the two second closing doors is also provided in the material channel 60. Specifically, the linear driving member includes a driving wheel 83 and a driven wheel 84, the driving wheel 83 and the driven wheel 84 are rotatably mounted at the bottom of the material channel 60, a synchronous belt 85 is arranged between the driving wheel 83 and the driven wheel 84, the material shelf 81 is fixedly connected with the synchronous belt 85 through a connecting seat, and the driving assembly further includes a forward and reverse rotation motor 86 for driving the driving wheel 83 to rotate. The material channel 60 is provided with guide rails 87 at both sides of the synchronous belt 85, and the material shelf 81 is slidably mounted on the guide rails 87. In order to control the forward and reverse rotation motor 86, a control switch for controlling the operation of the forward and reverse rotation motor 86 is arranged in the preparation room 30 and the operation part, so that the materials can be conveyed in the material channel 60 conveniently. In addition, in order to further avoid the pollution generated in the conveying process of the materials, at least one group of ultraviolet sterilizing lamps are arranged at the top of each material channel 60, and when the materials are specifically implemented, the materials channels 60 can be irradiated by the ultraviolet sterilizing lamps to kill the mixed bacteria and cut off a pollution source, so that the sterility of the operating room 50 is ensured.
In this embodiment, the animal in vitro cell laboratory further includes a positive pressure air supply pipe 10, the positive pressure air supply pipe 10 is disposed at the top of the animal in vitro cell laboratory, the dressing room 20, the preparation room 30, the pedestrian corridor 40, the top of the operation room 50 and the material channel 60 are respectively provided with a first air outlet, a second air outlet, a third air outlet, a fourth air outlet and a fifth air outlet, the air outlet end of the positive pressure air supply pipe 10 is respectively communicated with the first air outlet, the second air outlet, the third air outlet, the fourth air outlet and the fifth air outlet through a first air pipe 22, a second air pipe 32, a third air pipe 43, a fourth air pipe 53 and a fifth air pipe 61, so as to respectively convey fresh air into the dressing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60. In order to facilitate the air treatment to ensure that the operating room is in an aseptic environment, the air inlet end of the positive pressure air supply pipe 10 is also provided with at least one group of particle filtering components, and an ultraviolet sterilization component is arranged downstream of the particle filtering components. Specifically, particulate matter filtering component is including filtering cotton filltering section and active carbon filltering section, has filled respectively in filtering cotton filltering section and the active carbon filltering section and has filtered surface and active carbon, and filters the upstream that the active carbon filltering section was located to the cotton filltering section. The ultraviolet sterilization component comprises a plurality of ultraviolet sterilization lamp tubes arranged in the positive pressure blast pipe 10. In specific implementation, air is filtered through filter cotton and active carbon to intercept particulate matters in the air, and then the air is irradiated and sterilized through an ultraviolet sterilization lamp tube.
In this embodiment, in order to exhaust the polluted air, the bottom of the wall of the changing room 20, the preparation room 30, the people's corridor 40, the operation room 50 and the material channel 60 is provided with an air outlet, the air outlet is communicated with the external environment through an air exhaust pipeline 70, the air exhaust pipeline 70 is pre-embedded inside the wall, and an air exhaust fan is arranged in the air exhaust pipeline 70, so that the clean air in the changing room 20, the preparation room 30, the people's corridor 40, the operation room 50 and the material channel 60 flows from top to bottom and the pollutants are exhausted from the air outlet. Meanwhile, in order to further bring the pollutants into the operating room 50, the air outlet in the people corridor 40 is arranged at one end, close to the preparation room 30, of the people corridor 40 and is positioned at the bottom of the wall body, the third air outlet is arranged at one end, far away from the preparation room 30, of the people corridor 40 and is positioned at the top of the people corridor 40, so that the time of the operator passing through the people corridor 40 is prolonged, the air in the people corridor 40 always flows to one side of the preparation room 30, and when the operator enters the operating room 50 through the people corridor 40, the operator can be further cleaned, and the introduction of the pollutants is reduced. In practical applications, the air intake rate of the changing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 should be controlled to be greater than the air outlet rate, so as to ensure that the changing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 are always in a micro-positive pressure state, thereby improving the proximity thereof.
In this embodiment, to facilitate the culture of the isolated animal cells, the operating room 50 is further divided into a preparation chamber 51 and a cell chamber 52, the preparation chamber 51 is communicated with the outlet of the material passage 60 and directly communicated with the pedestrian passageway 40 through the fourth inlet 42, and the cell chamber 52 is disposed on the side of the operating room 50 away from the pedestrian passageway 40 to reduce the invasion of bacteria.
The animal isolated cell laboratory provided by the application is described in detail above. The description of the specific embodiments is only intended to facilitate an understanding of the methods of the present application and their core concepts. It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present application without departing from the principle of the present application, and such improvements and modifications also fall within the scope of the claims of the present application.
Claims (7)
1. Animal isolated cell laboratory, characterized in that: comprises a dressing room (20), a preparation room (30) and a pedestrian corridor (40), the dressing room (20) is provided with a first inlet (21), the preparation room (30) is communicated with the dressing room (20) through a second inlet (31), the corridor (40) is arranged perpendicular to the preparation room (30) and is communicated with the middle part of the preparation room (30) through a third inlet (41), two sides of the pedestrian corridor (40) are respectively provided with an operating room (50), the walking corridor (40) is provided with two fourth inlets (42) at one end far away from the preparation room (30), the fourth inlet (42) is used for communicating the pedestrian corridor (40) with an operation room (50), and a first sealing door is arranged at each of the first inlet (21), the second inlet (31), the third inlet (41) and the fourth inlet (42).
2. The animal ex vivo cell laboratory of claim 1, wherein: a linear material channel (60) is arranged between the preparation room (30) and each operation room (50), two ends of the material channel (60) are provided with a second sealing door, and material conveying equipment is arranged in the material channel (60).
3. The animal ex vivo cell laboratory of claim 2, wherein: the material conveying equipment comprises a material shelf (81) which is arranged in the material channel (60) in a sliding mode, a material trolley (82) is arranged on the material shelf (81), and a linear driving piece which is used for driving the material shelf (81) to move between the second closed doors is further arranged in the material channel (60).
4. The animal ex vivo cell laboratory of claim 2, wherein: still include malleation blast pipe (10), be equipped with first air outlet, second air outlet, third air outlet, fourth air outlet and fifth air outlet in dressing room (20), preparation room (30), pedestrian's corridor (40), operation room (50) and material passageway (60) respectively, the air-out end of malleation blast pipe (10) respectively through first tuber pipe (22), second tuber pipe (32), third tuber pipe (43), fourth tuber pipe (53) and fifth tuber pipe (61) with first air outlet, second air outlet, third air outlet, fourth air outlet and fifth air outlet intercommunication.
5. The animal ex vivo cell laboratory of claim 4, wherein: the air inlet end of the positive pressure air supply pipe (10) is also at least provided with a group of particle filtering components, and the downstream of the particle filtering components is provided with an ultraviolet sterilization component.
6. The animal ex vivo cell laboratory of claim 4, wherein: the wall body bottom of dressing room (20), prepare room (30), pedestrian corridor (40), operation room (50) and material passageway (60) all is equipped with the air exit, the air exit passes through exhaust duct (70) and external environment intercommunication, exhaust duct (70) are pre-buried inside the wall body just be equipped with the exhaust fan in exhaust duct (70).
7. The animal ex vivo cell laboratory of claim 6, wherein: the air outlet in people's line corridor (40) is located people's line corridor (40) is close to the one end of preparing room (30) and is located the wall body bottom, the third air outlet is located people's line corridor (40) is kept away from the one end of preparing room (30) and is located the top of people's line corridor (40).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111526159.3A CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111526159.3A CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114215395A true CN114215395A (en) | 2022-03-22 |
| CN114215395B CN114215395B (en) | 2023-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111526159.3A Active CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1587601A (en) * | 2004-08-02 | 2005-03-02 | 杭州博日科技有限公司 | Movable safety laboratory |
| JP2013240358A (en) * | 2013-08-26 | 2013-12-05 | Regene Pharm:Kk | Unit type cell culture facility |
| CN104847139A (en) * | 2015-04-13 | 2015-08-19 | 上海安集协康生物技术有限公司 | Clean cell production workshop |
| CN207266565U (en) * | 2017-09-08 | 2018-04-24 | 湖北天勤生物科技有限公司 | Animal Lab. barrier system |
| CN207686352U (en) * | 2017-12-19 | 2018-08-03 | 吉林和泽生物科技有限公司 | Stem cell aseptic workshop |
| CN209023616U (en) * | 2018-11-19 | 2019-06-25 | 上海原能细胞生物低温设备有限公司 | A kind of wall-penetrating type transferring device |
| CN209214025U (en) * | 2018-11-14 | 2019-08-06 | 广州皓康净化科技有限公司 | Laboratory air purification system |
-
2021
- 2021-12-14 CN CN202111526159.3A patent/CN114215395B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1587601A (en) * | 2004-08-02 | 2005-03-02 | 杭州博日科技有限公司 | Movable safety laboratory |
| JP2013240358A (en) * | 2013-08-26 | 2013-12-05 | Regene Pharm:Kk | Unit type cell culture facility |
| CN104847139A (en) * | 2015-04-13 | 2015-08-19 | 上海安集协康生物技术有限公司 | Clean cell production workshop |
| CN207266565U (en) * | 2017-09-08 | 2018-04-24 | 湖北天勤生物科技有限公司 | Animal Lab. barrier system |
| CN207686352U (en) * | 2017-12-19 | 2018-08-03 | 吉林和泽生物科技有限公司 | Stem cell aseptic workshop |
| CN209214025U (en) * | 2018-11-14 | 2019-08-06 | 广州皓康净化科技有限公司 | Laboratory air purification system |
| CN209023616U (en) * | 2018-11-19 | 2019-06-25 | 上海原能细胞生物低温设备有限公司 | A kind of wall-penetrating type transferring device |
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| Publication number | Publication date |
|---|---|
| CN114215395B (en) | 2023-09-19 |
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