CN114215395B - Animal ex vivo cell laboratory - Google Patents
Animal ex vivo cell laboratory Download PDFInfo
- Publication number
- CN114215395B CN114215395B CN202111526159.3A CN202111526159A CN114215395B CN 114215395 B CN114215395 B CN 114215395B CN 202111526159 A CN202111526159 A CN 202111526159A CN 114215395 B CN114215395 B CN 114215395B
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- Prior art keywords
- room
- air
- air outlet
- inlet
- corridor
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Classifications
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- E—FIXED CONSTRUCTIONS
- E04—BUILDING
- E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
- E04H1/00—Buildings or groups of buildings for dwelling or office purposes; General layout, e.g. modular co-ordination or staggered storeys
- E04H1/12—Small buildings or other erections for limited occupation, erected in the open air or arranged in buildings, e.g. kiosks, waiting shelters for bus stops or for filling stations, roofs for railway platforms, watchmen's huts or dressing cubicles
- E04H1/1277—Shelters for decontamination
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F3/00—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems
- F24F3/12—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling
- F24F3/16—Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatment; Apparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling by purification, e.g. by filtering; by sterilisation; by ozonisation
- F24F3/167—Clean rooms, i.e. enclosed spaces in which a uniform flow of filtered air is distributed
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F7/00—Ventilation
- F24F7/003—Ventilation in combination with air cleaning
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F7/00—Ventilation
- F24F7/04—Ventilation with ducting systems, e.g. by double walls; with natural circulation
- F24F7/06—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit
- F24F7/08—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit with separate ducts for supplied and exhausted air with provisions for reversal of the input and output systems
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
- F24F8/10—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering
- F24F8/108—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by separation, e.g. by filtering using dry filter elements
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F24—HEATING; RANGES; VENTILATING
- F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
- F24F8/00—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying
- F24F8/20—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation
- F24F8/22—Treatment, e.g. purification, of air supplied to human living or working spaces otherwise than by heating, cooling, humidifying or drying by sterilisation using UV light
Abstract
The application discloses an animal in-vitro cell laboratory, which relates to the technical field of biological laboratories and comprises a dressing room, a preparation room and a pedestrian corridor, wherein a first inlet is formed in the dressing room, the preparation room is communicated with the dressing room through a second inlet, the pedestrian corridor is perpendicular to the preparation room and is communicated with the middle part of the preparation room through a third inlet, two operation rooms are respectively arranged on two sides of the pedestrian corridor, two fourth inlets are formed in one end of the pedestrian corridor far away from the preparation room, the fourth inlets are used for communicating the pedestrian corridor with the operation rooms, and first sealing doors are arranged at the first inlet, the second inlet, the third inlet and the fourth inlet. The animal in-vitro cell laboratory structure line is reasonable in design, can effectively keep clean in a laboratory, and can greatly improve the efficiency of animal in-vitro cell culture.
Description
Technical Field
The application belongs to the technical field of biological laboratories, and particularly relates to an animal in-vitro cell laboratory.
Background
The isolated cell culture (animal cell culture) is carried out by taking the relevant tissue from the animal body, dispersing it into individual cells (using trypsin or collagenase) and then placing them in a suitable medium to allow the cells to grow and proliferate. Tissue cell culture techniques are primarily distinguished from other general laboratory work by the need to maintain aseptic operation, avoiding the effects of microorganisms and other deleterious factors.
At present, the design structure of a common animal cell in-vitro culture laboratory is complex, the design of a line is unreasonable, and the requirement of animal cell in-vitro culture cannot be met.
Disclosure of Invention
In order to solve the technical problems, the application aims to provide an in-vitro cell laboratory which is reasonable in structural line design and can ensure the in-vitro cell culture efficiency of animals.
In order to achieve the technical effects, the application adopts the following technical scheme:
animal cell laboratory in vitro, including changing room, readiness room and people's corridor, be equipped with first entry on the changing room, prepare the room through the second entry with change room intercommunication, people's corridor perpendicular to prepare the room setting and through the third entry with the middle part intercommunication between the readiness, the both sides of people's corridor respectively are provided with an operation room, people's corridor is keeping away from one end between the readiness is equipped with two fourth entrances, the fourth entrance is used for the intercommunication people's corridor and operation room, first entry, second entry, third entry and fourth entrance all are equipped with a first sealing door.
Further, a straight-line-shaped material channel is arranged between the preparation room and each operation room, two ends of the material channel are respectively provided with a second sealing door, and material conveying equipment is arranged in the material channel.
Further, the material conveying device comprises a material shelf which is slidably arranged in the material channel, a material trolley is arranged on the material shelf, and a linear driving piece which is used for driving the material shelf to move between the two second sealing doors is further arranged in the material channel.
Further, the clothes changing room is provided with a first air outlet, a second air outlet, a third air outlet, a fourth air outlet and a fifth air outlet in the clothes changing room, the preparation room, the pedestrian corridor, the operation room and the material channel respectively, and the air outlet end of the positive pressure air supplying pipe is communicated with the first air outlet, the second air outlet, the third air outlet, the fourth air outlet and the fifth air outlet through the first air pipe, the second air pipe, the third air pipe, the fourth air pipe and the fifth air pipe respectively.
Further, the air inlet end of the positive pressure air supply pipe is also provided with at least one group of particle filtering components, and the downstream of the particle filtering components is provided with an ultraviolet sterilization component.
Further, the wall body bottoms of the changing room, the preparation room, the pedestrian corridor, the operation room and the material channel are all provided with air outlets, the air outlets are communicated with the external environment through air exhaust pipelines, and the air exhaust pipelines are embedded in the wall body and are internally provided with air exhaust fans.
Further, the air outlet in the pedestrian corridor is arranged at one end of the pedestrian corridor, which is close to the preparation room, and is arranged at the bottom of the wall body, and the third air outlet is arranged at one end of the pedestrian corridor, which is far away from the preparation room, and is arranged at the top of the pedestrian corridor.
The beneficial effects of the application are as follows: according to the animal in-vitro cell laboratory, the dressing room, the preparation room, the pedestrian corridor and the operation room are arranged, and reasonable layout is carried out on the dressing room, the preparation room, the pedestrian corridor and the operation room, so that the in-vitro cell laboratory is compact in structure and reasonable in line, the requirement of animal in-vitro cell culture can be met, and the efficiency of animal in-vitro cell culture is improved. In addition, through setting up the material passageway that separates with the people's bank of china corridor and be used for carrying out automatic transport to the material for operating personnel and the material that get into the operation room divide the stream, have further reduced the difficulty of killing of this animal in vitro cell laboratory, have reduced the pollution probability between this operation room.
Drawings
FIG. 1 is a schematic diagram of the whole structure of an animal ex vivo cell laboratory according to an embodiment of the present application;
FIG. 2 is a schematic plan view of an in vitro animal cell laboratory according to an embodiment of the present application;
fig. 3 is a schematic structural diagram of a linear driving member of an animal ex vivo cell laboratory according to an embodiment of the application.
The figure indicates: 10-positive pressure blast pipes, 20-dressing rooms, 21-first inlets, 22-first blast pipes, 30-preparation rooms, 31-second inlets, 32-second blast pipes, 40-pedestrian corridors, 41-third inlets, 42-fourth inlets, 43-third blast pipes, 50-operation rooms, 51-preparation rooms, 52-cell rooms, 53-fourth blast pipes, 60-material passages, 61-fifth blast pipes, 70-exhaust pipes, 81-material shelves, 82-material trolleys, 83-driving wheels, 84-driven wheels, 85-synchronous belts, 86-reversible motors and 87-guide rails.
Detailed Description
The application will be further described with reference to examples and drawings, to which reference is made, but which are not intended to limit the scope of the application.
As shown in fig. 1-3, the present embodiment provides an animal ex vivo cell laboratory, which includes a dressing room 20, a preparation room 30, a pedestrian corridor 40, and an operation room 50, wherein the dressing room 20 is provided with a first inlet 21. The preparation room 30 is communicated with the dressing room 20 through the second inlet 31, the pedestrian corridor 40 is arranged perpendicular to the preparation room 30 and is communicated with the middle of the preparation room 30 through the third inlet 41, the operation room 50 is provided with two and is respectively positioned at two sides of the pedestrian corridor 40, the pedestrian corridor 40 is provided with two fourth inlets 42 at one end far away from the preparation room 30, the fourth inlets 42 are used for communicating the pedestrian corridor 40 with the operation room 50, and the first inlets 21, the second inlets 31, the third inlets 41 and the fourth inlets 42 are all provided with a first sealing door. In order to facilitate the transportation of materials, equipment and the like, a linear material channel 60 is arranged between the preparation room 30 and each operation room 50, two ends of the material channel 60 are respectively provided with a second sealing door, and the material channel 60 is internally provided with material transportation equipment. In the animal in-vitro cell laboratory, the route of an operator entering the operation room 50 is a dressing room 20-a preparation room 30-a pedestrian corridor 40-the operation room 50, and the route of a material entering the operation room 50 is a dressing room 20-a preparation room 30-a material channel 60-the operation room 50, so that the animal in-vitro cell laboratory can split the operator and the material, the disinfection difficulty can be further reduced, and the cleanliness of the operation room 50 is improved.
In this embodiment, in order to facilitate the delivery of materials, the material delivery apparatus includes a material shelf 81 slidably mounted in the material passage 60, and a material cart 82 is provided on the material shelf 81, and the material cart 82 is preferably a cart of a multi-layered structure for placing materials, instruments or equipment. A linear drive is also provided in the material passageway 60 for driving the material shelf 81 between the two second closure doors. Specifically, the linear driving member includes a driving wheel 83 and a driven wheel 84, the driving wheel 83 and the driven wheel 84 are rotatably mounted at the bottom of the material channel 60, a synchronous belt 85 is disposed between the driving wheel 83 and the driven wheel 84, the material shelf 81 is fixedly connected with the synchronous belt 85 through a connecting seat, and the driving assembly further includes a forward and reverse rotation motor 86 for driving the driving wheel 83 to rotate. The material channel 60 is provided with guide rails 87 on both sides of the timing belt 85, and the material shelf 81 is slidably mounted on the guide rails 87. To facilitate control of the reversible motor 86, control switches for controlling operation of the reversible motor 86 are provided in the preparation room 30 and the operating member to facilitate control of the material transport in the material passage 60. In addition, in order to further avoid pollution of the materials in the conveying process, at least one group of ultraviolet sterilizing lamps are arranged at the top of each material channel 60, and when the method is implemented, the ultraviolet sterilizing lamps can be used for irradiating the material channels 60 to kill miscellaneous bacteria and cut off pollution sources, so that the sterility of the operation room 50 is ensured.
In this embodiment, the animal ex-vivo cell laboratory further includes a positive pressure air supply pipe 10, where the positive pressure air supply pipe 10 is disposed at the top of the animal ex-vivo cell laboratory, and the tops of the dressing room 20, the preparation room 30, the man-made corridor 40, the operation room 50 and the material channel 60 are respectively provided with a first air outlet, a second air outlet, a third air outlet, a fourth air outlet and a fifth air outlet, and the air outlet end of the positive pressure air supply pipe 10 is respectively communicated with the first air outlet, the second air outlet, the third air outlet, the fourth air outlet and the fifth air outlet through the first air pipe 22, the second air pipe 32, the third air pipe 43, the fourth air pipe 53 and the fifth air pipe 61, so as to convey fresh air into the dressing room 20, the preparation room 30, the man-made corridor 40, the operation room 50 and the material channel 60. In order to facilitate the treatment of air to ensure that the operation room is in a sterile environment, the air inlet end of the positive pressure air supply pipe 10 is further provided with at least one group of particulate matter filtering components, and the downstream of the particulate matter filtering components is provided with an ultraviolet sterilization component. Specifically, particulate matter filter subassembly includes filter cotton filter segment and active carbon filter segment, and filter cotton filter segment and active carbon filter segment are filled with filtering face and active carbon respectively, and the filter cotton filter segment locates the upper reaches of active carbon filter segment. The ultraviolet sterilization component comprises a plurality of ultraviolet sterilization lamp tubes arranged in the positive pressure blast pipe 10. In the specific implementation, firstly, air is filtered through filter cotton and active carbon to intercept particulate matters in the air, and then the air is subjected to irradiation sterilization by an ultraviolet sterilization lamp tube.
In this embodiment, in order to facilitate the discharge of the polluted air, the bottoms of the walls of the dressing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 are all provided with air outlets, the air outlets are communicated with the external environment through the air exhaust pipes 70, the air exhaust pipes 70 are embedded in the walls and the air exhaust pipes 70 are provided with air blowers, so that the clean air in the dressing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 flows from top to bottom and discharges the pollutants from the air outlets. Meanwhile, in order to further bring the pollutants into the operation room 50, the air outlet in the pedestrian corridor 40 is arranged at one end of the pedestrian corridor 40 close to the preparation room 30 and is positioned at the bottom of the wall body, and the third air outlet is arranged at one end of the pedestrian corridor 40 far away from the preparation room 30 and is positioned at the top of the pedestrian corridor 40, so that the time for the operator to pass through the pedestrian corridor 40 is prolonged, the air in the pedestrian corridor 40 always flows to one side of the preparation room 30, and when the operator enters the operation room 50 through the pedestrian corridor 40, the air can be further cleaned to reduce the bringing of the pollutants. In practice, the air intake rates of the dressing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 should be controlled to be greater than the air outlet rate, so as to ensure that the dressing room 20, the preparation room 30, the pedestrian corridor 40, the operation room 50 and the material channel 60 are always in a micro-positive pressure state, thereby improving the proximity degree thereof.
In this embodiment, to facilitate the culture of isolated animal cells, the operation room 50 is further divided into a preparation room 51 and a cell room 52, wherein the preparation room 51 is communicated with the outlet of the material passage 60 and is directly communicated with the pedestrian corridor 40 through the fourth inlet 42, and the cell room 52 is disposed on the side of the operation room 50 away from the pedestrian corridor 40 to reduce bacterial invasion.
The animal ex vivo cell laboratory provided by the application is described in detail above. The description of the specific embodiments is only intended to aid in understanding the method of the present application and its core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the application can be made without departing from the principles of the application and these modifications and adaptations are intended to be within the scope of the application as defined in the following claims.
Claims (3)
1. Animal isolated cell laboratory, its characterized in that: the automatic clothes changing machine comprises a clothes changing room (20), a preparation room (30) and a pedestrian corridor (40), wherein a first inlet (21) is formed in the clothes changing room (20), the preparation room (30) is communicated with the clothes changing room (20) through a second inlet (31), the pedestrian corridor (40) is perpendicular to the preparation room (30) and is communicated with the middle of the preparation room (30) through a third inlet (41), two operation rooms (50) are respectively formed in two sides of the pedestrian corridor (40), two fourth inlets (42) are formed in one ends, far away from the preparation room (30), of the pedestrian corridor (40), the fourth inlets (42) are used for communicating the pedestrian corridor (40) with the operation rooms (50), and a first sealing door is arranged at each of the first inlet (21), the second inlet (31), the third inlet (41) and the fourth inlet (42);
a linear material channel (60) is arranged between the preparation room (30) and each operation room (50), two ends of the material channel (60) are respectively provided with a second sealing door, and material conveying equipment is arranged in the material channel (60);
the material conveying equipment comprises a material shelf (81) which is slidably arranged in the material channel (60), a material trolley (82) is arranged on the material shelf (81), and a linear driving piece which is used for driving the material shelf (81) to move between the two second closed doors is also arranged in the material channel (60);
the clothes changing room is characterized by further comprising a positive pressure air supply pipe (10), wherein a first air outlet, a second air outlet, a third air outlet, a fourth air outlet and a fifth air outlet are respectively arranged in the clothes changing room (20), the preparation room (30), the pedestrian corridor (40), the operation room (50) and the material channel (60), and the air outlet end of the positive pressure air supply pipe (10) is communicated with the first air outlet, the second air outlet, the third air outlet, the fourth air outlet and the fifth air outlet through a first air pipe (22), a second air pipe (32), a third air pipe (43), a fourth air pipe (53) and a fifth air pipe (61) respectively;
the clothes changing room is characterized in that air outlets are formed in the bottoms of the walls of the clothes changing room (20), the preparation room (30), the pedestrian corridor (40), the operation room (50) and the material channel (60), the air outlets are communicated with the external environment through air exhaust pipelines (70), and the air exhaust pipelines (70) are embedded in the walls and are internally provided with air exhaust fans.
2. The animal ex vivo cell laboratory of claim 1, wherein: the air inlet end of the positive pressure air supply pipe (10) is also provided with at least one group of particle filtering components, and the downstream of the particle filtering components is provided with an ultraviolet sterilization component.
3. The animal ex vivo cell laboratory of claim 1, wherein: the air outlet in the pedestrian corridor (40) is arranged at one end of the pedestrian corridor (40) close to the preparation room (30) and is arranged at the bottom of the wall body, and the third air outlet is arranged at one end of the pedestrian corridor (40) away from the preparation room (30) and is arranged at the top of the pedestrian corridor (40).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111526159.3A CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111526159.3A CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114215395A CN114215395A (en) | 2022-03-22 |
| CN114215395B true CN114215395B (en) | 2023-09-19 |
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ID=80701739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111526159.3A Active CN114215395B (en) | 2021-12-14 | 2021-12-14 | Animal ex vivo cell laboratory |
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| Country | Link |
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| CN (1) | CN114215395B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1587601A (en) * | 2004-08-02 | 2005-03-02 | 杭州博日科技有限公司 | Movable safety laboratory |
| JP2013240358A (en) * | 2013-08-26 | 2013-12-05 | Regene Pharm:Kk | Unit type cell culture facility |
| CN104847139A (en) * | 2015-04-13 | 2015-08-19 | 上海安集协康生物技术有限公司 | Clean cell production workshop |
| CN207266565U (en) * | 2017-09-08 | 2018-04-24 | 湖北天勤生物科技有限公司 | Animal Lab. barrier system |
| CN207686352U (en) * | 2017-12-19 | 2018-08-03 | 吉林和泽生物科技有限公司 | Stem cell aseptic workshop |
| CN209023616U (en) * | 2018-11-19 | 2019-06-25 | 上海原能细胞生物低温设备有限公司 | A kind of wall-penetrating type transferring device |
| CN209214025U (en) * | 2018-11-14 | 2019-08-06 | 广州皓康净化科技有限公司 | Laboratory air purification system |
-
2021
- 2021-12-14 CN CN202111526159.3A patent/CN114215395B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1587601A (en) * | 2004-08-02 | 2005-03-02 | 杭州博日科技有限公司 | Movable safety laboratory |
| JP2013240358A (en) * | 2013-08-26 | 2013-12-05 | Regene Pharm:Kk | Unit type cell culture facility |
| CN104847139A (en) * | 2015-04-13 | 2015-08-19 | 上海安集协康生物技术有限公司 | Clean cell production workshop |
| CN207266565U (en) * | 2017-09-08 | 2018-04-24 | 湖北天勤生物科技有限公司 | Animal Lab. barrier system |
| CN207686352U (en) * | 2017-12-19 | 2018-08-03 | 吉林和泽生物科技有限公司 | Stem cell aseptic workshop |
| CN209214025U (en) * | 2018-11-14 | 2019-08-06 | 广州皓康净化科技有限公司 | Laboratory air purification system |
| CN209023616U (en) * | 2018-11-19 | 2019-06-25 | 上海原能细胞生物低温设备有限公司 | A kind of wall-penetrating type transferring device |
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| Publication number | Publication date |
|---|---|
| CN114215395A (en) | 2022-03-22 |
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