CN114175966A - Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method - Google Patents
Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method Download PDFInfo
- Publication number
- CN114175966A CN114175966A CN202111568684.1A CN202111568684A CN114175966A CN 114175966 A CN114175966 A CN 114175966A CN 202111568684 A CN202111568684 A CN 202111568684A CN 114175966 A CN114175966 A CN 114175966A
- Authority
- CN
- China
- Prior art keywords
- parts
- culture medium
- pleurotus eryngii
- water
- bran
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 43
- 244000252132 Pleurotus eryngii Species 0.000 title claims abstract description 26
- 235000001681 Pleurotus eryngii Nutrition 0.000 title claims abstract description 26
- 238000012364 cultivation method Methods 0.000 title claims abstract description 10
- 241001474374 Blennius Species 0.000 claims description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 26
- 241000233866 Fungi Species 0.000 claims description 19
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 13
- 239000004571 lime Substances 0.000 claims description 13
- 239000002023 wood Substances 0.000 claims description 13
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 12
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 244000061456 Solanum tuberosum Species 0.000 claims description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 3
- 229920000615 alginic acid Polymers 0.000 claims description 3
- 235000010443 alginic acid Nutrition 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000206607 Porphyra umbilicalis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000008570 general process Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000012204 lemonade/lime carbonate Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to the technical field of edible mushroom cultivation, in particular to a culture medium for a pleurotus eryngii cultivation seed and a cultivation method for the pleurotus eryngii.
Description
Technical Field
The invention relates to the technical field of edible mushroom cultivation, in particular to a culture medium for pleurotus eryngii cultivars and a pleurotus eryngii cultivation method.
Background
Edible fungi are large fungi for human consumption, are food rich in nutrition and have food therapy value, are rich in protein, vitamins and various amino acids essential to human bodies, and are increasingly favored by consumers. Pleurotus eryngii, also called Pleurotus eryngii, has fleshy mass, long storage time and transportation resistance, and is a high-quality edible mushroom with great development potential. At present, the large-scale and annual production is carried out in Fujian, Shanghai, Jiangsu, Shandong and other places in China, and the method becomes one of the most-productive industrial cultivation types in China.
The main cultivation materials of the pleurotus eryngii generally take sawdust, corncobs, corn flour, bran and bean pulp as main materials. Along with the increasing expansion of domestic edible fungus production scale, the problem of shortage of the cultivation main materials is increasingly prominent, particularly the price of the cultivation main materials is also increased, the gradual increase of the production cost of the edible fungi is promoted, and great production pressure is caused to enterprises.
The sea area of China is wide, and the seaweed has abundant seaweed resources, such as kelp, laver and the like. A large amount of seaweed residues are left after seaweed processing, and the main components of the seaweed residues comprise seaweed crude fibers, proteins, seaweed polysaccharides and the like. The seaweed residues are used for cultivating the edible fungi, so that the problem of shortage of the main cultivation materials can be relieved, the environmental pollution can be reduced, the comprehensive utilization of resources is improved, and the like.
Disclosure of Invention
The invention aims to provide a culture medium for pleurotus eryngii cultivars and a pleurotus eryngii cultivation method. The invention starts from the seaweed residues, aims to utilize the seaweed residues, solves the problem of environmental pollution caused by the seaweed residues, changes waste into valuable, and is applied to the cultivation of edible fungi to improve the yield of the edible fungi.
In order to realize the purpose of the invention, the following technical means are specifically adopted:
a culture medium for Pleurotus eryngii cultivars contains 3-5% of seaweed residues by mass ratio.
Preferably, the seaweed residue is kelp residue obtained by removing algin.
Preferably, the formula of the culture medium for the cultivars comprises the following components in parts by mass: 38-40 parts of miscellaneous wood chips, 28-32 parts of corncobs, 23-27 parts of bran, 3-5 parts of seaweed residues, 0.8-1.2 parts of lime, 0.8-1.2 parts of calcium carbonate and water; the water content of the culture medium for the cultivars is 60-65%.
Preferably, the formula of the culture medium for the cultivars comprises the following components in parts by mass: 38-40 parts of miscellaneous wood chips, 30 parts of corncobs, 25 parts of bran, 3-5 parts of seaweed residues, 1 part of lime, 1 part of calcium carbonate and water; the water content of the culture medium for the cultivars is 60-65%.
A cultivation method of pleurotus eryngii comprises the following steps:
(1) taking pleurotus eryngii strains, inoculating the pleurotus eryngii strains into a mother strain culture medium, and culturing at 25-30 ℃;
(2) inoculating the cultured mother strain into a stock culture medium, and then culturing at 20-23 ℃;
(3) selecting an original seed with white hyphae and excellent growth vigor, inoculating the original seed into a fungus bag prepared from the culture medium of any one of claims 1 to 4, and then culturing the original seed in a dark place at the temperature of 20 to 23 ℃;
(4) and (4) fruiting the physiologically mature fungus.
Preferably, the mother culture medium formula is as follows: 180-220 parts of potato, 18-22 parts of glucose, 13-17 parts of agar and 900-1100 parts of water.
Preferably, the mother culture medium formula is as follows: 200 parts of potato, 20 parts of glucose, 15 parts of agar and 1000 parts of water.
Preferably, the stock culture medium configuration method comprises the following steps: boiling the branches in 1-1.2% white sugar water for 30 minutes, taking out, uniformly mixing with the soaked bran, filling the mixture into a container, filling the space in the container with the mixed wood chips with the water content of 60-63% and the bran with the water content of 60-63%, and sterilizing by high-pressure steam.
Advantageous effects
The seaweed residues are added into the culture medium of the cultivated species of the edible fungi, so that the formation of sporocarp and the improvement of yield are facilitated, the problem of environmental pollution caused by the seaweed residues is solved, and the cultivation cost is reduced. And by adopting the culture medium of the cultivar, the fruiting is normal, the yield is stable, and the method is very suitable for large-scale production.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The water content in the following examples refers to the mass of water contained in a substance/the total mass of the substance. For example, the moisture content of the soaked bran refers to the mass of moisture contained in the soaked bran/the mass of the soaked bran.
First, culture medium and preparation process
1. Mother culture medium
The formula is as follows: potato 200g, glucose 20g, agar 15g, water 1000ml, pH natural.
The manufacturing process comprises the following steps: accurately weighing various nutrients, preparing slant culture medium according to general process, and sterilizing.
The preparation process of the slant culture medium comprises the following steps: peeling potato 200g, cutting into small pieces, mixing with water 1000ml, boiling for 9-11 min until potato is soft but not rotten, filtering to obtain filtrate, adding glucose 20g and agar 15g into the filtrate, boiling until agar is completely dissolved, adding water to culture solution 1000ml, then 1000ml of culture liquid is subpackaged into test tubes while the culture liquid is hot, the volume of each subpackaged culture liquid is 1/4-1/3 of the length of each test tube, then sealing the opening of the test tube with a cotton plug, sterilizing the test tube filled with the culture liquid in a sterilizing pot with the pressure of 1.0kg/cm and the temperature of 121 ℃ for 30min, sterilizing, obliquely placing and taking out the test tubes, transferring into an inoculation box after the culture medium is solidified and cooled, placing 3-5 test tubes into an incubator at 29-31 ℃ for culturing for 3 days, and forming a mother culture medium for inoculation if no foreign bacteria appear.
2. Stock culture medium
The formula is as follows: 6-8 kg of branches, 250-500 g of white sugar, 4-5 kg of miscellaneous wood chips, 2-3 kg of bran and water.
The branches in the embodiment are peeled branches of poplar trees.
The manufacturing process comprises the following steps: boiling the branches in 1% white sugar water for 30 minutes, taking out the branches, uniformly mixing the branches with the soaked bran, wherein the water content of the soaked bran is 60% -63%, bottling the mixture, and finally filling the gaps in the bottle with the mixed sawdust with the water content of 60% -63% and the bran with the water content of 60% -63%. Sterilizing with high pressure steam for use.
3. Culture medium for cultivation
The formula is as follows: 34-43% of sawdust, 30% of corncobs, 25% of bran, 0-9% of seaweed residues, 1% of lime and 1% of calcium carbonate.
The specific implementation mode is as follows:
formula 1: 43% of sawdust, 30% of corncobs, 25% of bran, 0% of seaweed residues, 1% of lime and 1% of calcium carbonate.
And (2) formula: 40% of miscellaneous wood chips, 30% of corncobs, 25% of bran, 3% of seaweed residues, 1% of lime and 1% of calcium carbonate.
And (3) formula: 38% of miscellaneous wood chips, 30% of corncobs, 25% of bran, 5% of seaweed residues, 1% of lime and 1% of calcium carbonate.
And (4) formula: 36% of miscellaneous wood chips, 30% of corncobs, 25% of bran, 7% of seaweed residues, 1% of lime and 1% of calcium carbonate.
And (5) formula: 34% of sawdust, 30% of corncobs, 25% of bran, 9% of seaweed residues, 1% of lime and 1% of calcium carbonate.
The residue of seaweed used in this example is the residue of seaweed from which algin was removed.
The manufacturing process comprises the following steps: accurately weighing various ingredients of the formula 1-5. Firstly, uniformly stirring the mixed wood chips, the corncobs, the bran, the seaweed residues (if any) and the bran, then adding lime and calcium carbonate, adding water into the materials for multiple times, and fully and uniformly stirring to ensure that the water content of the culture material is 60-65%. And (3) using polypropylene plastic bags, wherein each bag is filled with 1.1-1.2 kg of materials, and sterilizing for 2 hours by using high-pressure steam.
Second, production process
1. Inoculating Pleurotus eryngii strain (fruiting body of Pleurotus eryngii strain provided by edible fungus Co., Ltd. of five trees in Futian province, Zhannan county, with drumstick shape) in the mother culture medium, and culturing at 28 deg.C until mycelium grows over the whole inclined plane.
2. Inoculating the cultured mother strain into an original strain culture medium, and then placing a strain bottle at 20-23 ℃ for culturing until the mycelium grows over the whole bottle body, wherein the culture period is 25-28 days.
3. Selecting stock with white hypha and excellent growth vigor, opening the sealing cover of the fungus bottle, clamping 1 branch, and inoculating in culture medium. Then, the fungus bag is placed at the temperature of 20-23 ℃ and is cultured in the dark, and generally 28-30 days are needed.
4. And (3) transferring the physiologically mature fungus bags into a mushroom growing room, placing the five fungus bags in the same mushroom growing room, and adjusting external conditions such as temperature, humidity, carbon dioxide concentration, illumination and the like according to a conventional mode to enable the fungus bags to grow mushrooms normally.
Production application
The fungus bags are moved into the mushroom house for about 20 days, and normal harvest can be realized when the length of the fungus stalks is 10-12 cm. And counting the first tide of pleurotus eryngii. Each formula was randomly selected from 3 groups of 10 bags each, and the statistical average yield and biological efficiency index are shown in table 1.
TABLE 1 average yield of Pleurotus eryngii
As can be seen from Table 1, the yield of fruiting bodies of formula 2 (the addition amount of the seaweed residues is 3%) and formula 3 (the addition amount of the seaweed residues is 5%) is significantly improved compared with formula 1, which indicates that the addition of a proper amount of the seaweed residues in the culture medium is beneficial to the formation of the fruiting bodies and the improvement of the yield.
Compared with the formula 1, the fruiting body yield of the formula 4 (the addition amount of the seaweed residues is 7%) and the formula 5 (the addition amount of the seaweed residues is 9%) is slightly reduced, which indicates that the excessive seaweed residues are not beneficial to the growth of the fruiting body.
The addition of 3% and 5% of seaweed residues in the culture material is beneficial to the formation of pleurotus eryngii fruiting bodies and the improvement of yield, so that the more suitable formulas are formula 2 and formula 3. And (2) formula: 40% of miscellaneous wood chips, 30% of corncobs, 25% of bran, 3% of seaweed residues, 1% of lime and 1% of calcium carbonate. And (3) formula: 38% of miscellaneous wood chips, 30% of corncobs, 25% of bran, 5% of seaweed residues, 1% of lime and 1% of calcium carbonate.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. The pleurotus eryngii cultivar culture medium is characterized by comprising 3-5% of seaweed residues by mass.
2. The cultivar medium of claim 1, wherein the seaweed residue is kelp residue obtained by removing algin.
3. The cultivar culture medium according to claim 1, wherein the cultivar culture medium is formulated in the following parts by mass: 38-40 parts of miscellaneous wood chips, 28-32 parts of corncobs, 23-27 parts of bran, 3-5 parts of seaweed residues, 0.8-1.2 parts of lime, 0.8-1.2 parts of calcium carbonate and water; the water content of the culture medium for the cultivars is 60-65%.
4. The cultivar culture medium according to claim 2, wherein the cultivar culture medium is formulated in the following parts by mass: 38-40 parts of miscellaneous wood chips, 30 parts of corncobs, 25 parts of bran, 3-5 parts of seaweed residues, 1 part of lime, 1 part of calcium carbonate and water; the water content of the culture medium for the cultivars is 60-65%.
5. A cultivation method of pleurotus eryngii is characterized by comprising the following steps:
(1) taking pleurotus eryngii strains, inoculating the pleurotus eryngii strains into a mother strain culture medium, and culturing at 25-30 ℃;
(2) inoculating the cultured mother strain into a stock culture medium, and then culturing at 20-23 ℃;
(3) selecting an original seed with white hyphae and excellent growth vigor, inoculating the original seed into a fungus bag prepared from the culture medium of any one of claims 1 to 4, and then culturing the original seed in a dark place at the temperature of 20 to 23 ℃;
(4) and (4) fruiting the physiologically mature fungus.
6. The cultivation method according to claim 5, wherein the mother culture medium formula is: 180-220 parts of potato, 18-22 parts of glucose, 13-17 parts of agar and 900-1100 parts of water.
7. The cultivation method according to claim 6, wherein the mother culture medium formula is: 200 parts of potato, 20 parts of glucose, 15 parts of agar and 1000 parts of water.
8. The cultivation method according to claim 5, wherein the stock culture medium is prepared by: boiling the branches in 1-1.2% white sugar water for 30 minutes, taking out, uniformly mixing with the soaked bran, filling the mixture into a container, filling the space in the container with the mixed wood chips with the water content of 60-63% and the bran with the water content of 60-63%, and sterilizing by high-pressure steam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111568684.1A CN114175966A (en) | 2021-12-21 | 2021-12-21 | Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111568684.1A CN114175966A (en) | 2021-12-21 | 2021-12-21 | Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114175966A true CN114175966A (en) | 2022-03-15 |
Family
ID=80544605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111568684.1A Pending CN114175966A (en) | 2021-12-21 | 2021-12-21 | Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114175966A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115005009A (en) * | 2022-04-25 | 2022-09-06 | 南京吾悦农业科技有限公司 | Pleurotus eryngii solid strain and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061777A (en) * | 2006-04-28 | 2007-10-31 | 刘魁 | Pleurotus eryngii cultivating material |
| CN106938944A (en) * | 2017-02-28 | 2017-07-11 | 山东七河生物科技股份有限公司 | Pleurotus eryngii industrial high yield cultivating method |
| CN111543252A (en) * | 2020-05-08 | 2020-08-18 | 福建环海生物科技股份有限公司 | Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method |
-
2021
- 2021-12-21 CN CN202111568684.1A patent/CN114175966A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101061777A (en) * | 2006-04-28 | 2007-10-31 | 刘魁 | Pleurotus eryngii cultivating material |
| CN106938944A (en) * | 2017-02-28 | 2017-07-11 | 山东七河生物科技股份有限公司 | Pleurotus eryngii industrial high yield cultivating method |
| CN111543252A (en) * | 2020-05-08 | 2020-08-18 | 福建环海生物科技股份有限公司 | Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method |
Non-Patent Citations (1)
| Title |
|---|
| 朱梦霞等: "利用海藻渣栽培食用菌的研究", 《现代食品》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115005009A (en) * | 2022-04-25 | 2022-09-06 | 南京吾悦农业科技有限公司 | Pleurotus eryngii solid strain and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101611767B (en) | Method for producing microbial fermentation bait for sea cucumbers | |
| CN106316693A (en) | Biological humic acid fertilizer and preparing method thereof | |
| CN104641942A (en) | Method for cultivating oyster mushroom on mulberry twigs | |
| CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
| CN103621316A (en) | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material | |
| CN106718021A (en) | A kind of yield Volvaria volvacea cultivation method high | |
| CN102726210B (en) | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus | |
| CN107047061B (en) | Efficient method for cultivating lucid ganoderma and culture medium thereof | |
| CN111492895A (en) | Poplar sawdust composite culture medium suitable for hypsizigus marmoreus planting and preparation method thereof | |
| CN111066574A (en) | Method for preparing Lepista sordida cultivars by using mushroom dregs | |
| CN102786334A (en) | Culture medium for culturing edible fungus production mother seeds | |
| CN114175966A (en) | Pleurotus eryngii cultivar culture medium and pleurotus eryngii cultivation method | |
| CN101658102A (en) | Cultivating method of pleurotus ferulae | |
| CN103704018A (en) | Method for preparing pleurotus salmoneostramineus liquid spawns with yellow serofluid as principal raw materials | |
| CN103621310A (en) | Wild beef mushroom successfully trained and cultivated by using mountain grass and mushroom residues | |
| CN110229757A (en) | One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development | |
| CN115039636A (en) | Culture medium for cultivating tremella and preparation method thereof | |
| CN108207493B (en) | Straw rotting type edible fungus liquid strain culture medium, preparation method and application | |
| KR20180122789A (en) | Medium composite of shiitake and cultivation method using thereof | |
| CN112931047A (en) | Method for manufacturing mushroom sticks of bag-cultivated mushrooms | |
| CN105410034B (en) | A kind of compound accelerant of cordyceps sinensis cultivation and preparation method thereof | |
| CN109874600A (en) | A kind of seafood mushroom culture substrate and preparation method thereof | |
| CN114424731B (en) | Lentinus edodes culture medium and lentinus edodes cultivation method | |
| CN109673386A (en) | A kind of Lepista sordida bacterium culture medium and preparation method and cultural method | |
| CN112481138B (en) | Preparation method of high-yield polysaccharide based on deep fermentation of sweet potato wastewater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220315 |