CN114174833A

CN114174833A - Analysis method of blood cell analyzer and blood cell analyzer

Info

Publication number: CN114174833A
Application number: CN201980098902.4A
Authority: CN
Inventors: 程蛟; 滕锦
Original assignee: Shenzhen Mindray Animal Medical Technology Co Ltd
Current assignee: Shenzhen Mindray Animal Medical Technology Co Ltd
Priority date: 2019-09-05
Filing date: 2019-09-05
Publication date: 2022-03-11
Also published as: WO2021042319A1

Abstract

An analysis method (100) of a blood cell analyzer and a blood cell analyzer, the analysis method (100) of the blood cell analyzer comprising: discharging air bubbles in a pre-heated bath of the blood cell analyzer after the blood cell analyzer exits from the sleep (S110); and sending the diluted solution in the preheated cell discharged with the bubbles to a counting cell of a blood cell analyzer for counting and classifying white blood cells (S120). According to the analysis method (100) of the blood cell analyzer and the blood cell analyzer, the bubbles in the preheating pool are discharged after the blood cell analyzer is out of dormancy, so that the blood cell analyzer is based on the diluent with bubbles removed when the blood cell analyzer counts and classifies white blood cells, the white blood cell counting and classifying result cannot be influenced due to the problem of bubbles, and the reliability of the blood analysis result is improved.

Description

Analysis method of blood cell analyzer and blood cell analyzer

Description

Technical Field

The application relates to the technical field of blood cell analyzers, in particular to an analysis method of a blood cell analyzer and the blood cell analyzer.

Background

In the blood cell analyzer, if four or five classifications of the impedance method are required to be realized in the blood sample analysis process, the diluent is usually required to be heated to reach the reaction temperature required by the four or five classifications. The diluent is changed from low temperature to high temperature, gas dissolved in the diluent is separated out due to the change of temperature difference and exists in the preheating pool in the form of bubbles, and the separated amount of the gas is increased along with the prolonging of the dormancy time of the blood cell analyzer. Due to the different compressibility of the gas and the liquid, the generation of the bubbles can cause instability of a syringe of the blood cell analyzer in quantitative processes such as sample collection, diluent addition and the like, thereby affecting the measurement result of the blood cell analyzer.

Disclosure of Invention

The present application is proposed to solve the above problems. The application provides an analysis scheme of blood cell analysis appearance, it is through will preheating the bubble discharge in the pond after blood cell analysis appearance withdraws from the dormancy for blood cell analysis appearance is based on the diluent that has removed the bubble when carrying out leucocyte count and classification, therefore can not lead to influencing leucocyte count and classification result because of the bubble problem, has improved the reliability of blood analysis result. The analysis scheme of the blood cell analyzer proposed in the present application will be briefly described below, and more details will be described in the following detailed description with reference to the drawings.

In one aspect of the present application, there is provided an analysis method of a blood cell analyzer, the analysis method including: discharging air bubbles in a pre-heated cell of the blood cell analyzer after the blood cell analyzer exits from sleep; and sending the diluent in the preheated cell discharged with the bubbles to a counting cell of the blood cell analyzer for counting and classifying white blood cells.

In one embodiment of the present application, the bubble discharge process in the preheating bath includes: sucking out a part of the diluent in the preheating tank so that the bubbles are retained at the top of the preheating tank where the preheating tank is emptied; and supplementing new diluent to the preheating pool to refill the preheating pool and discharge the gas at the top of the preheating pool.

In one embodiment of the present application, the bubble discharge process in the preheating bath further includes: the injector of the blood cell analyzer is communicated with the preheating pool, and part of the diluent is sucked out of the preheating pool by the injector so that the top of the preheating pool is emptied; the injector discharges the diluent sucked out of the preheating pool through a first pipeline; the injector is communicated with the diluent barrel, and new diluent is sucked out of the diluent barrel by the injector to be used for supplementing new diluent to the preheating pool; and the injector is communicated with the preheating tank, and new diluent sucked out from the diluent barrel is replenished into the preheating tank by the injector so as to refill the preheating tank.

In one embodiment of the present application, the analysis method further comprises: after the blood cell analyzer is put to sleep again, the temperature of the preheating tank is lowered.

In one embodiment of the present application, the analysis method further comprises: timing a time at which the blood cell analyzer enters the sleep state after the blood cell analyzer enters the sleep state again; and adjusting the temperature of the pre-heating tank based on the length of time for which the blood cell analyzer is put to sleep so as to reduce the amount of gas evolved by the diluent in the pre-heating tank during sleep of the blood cell analyzer.

In one embodiment of the present application, the adjusting the temperature of the pre-heating bath based on the length of time the blood cell analyzer is put to sleep includes: gradually reducing the temperature of the pre-heating tank as the time for which the blood cell analyzer is put to sleep becomes longer.

In one embodiment of the present application, the adjusting the temperature of the pre-heating bath based on the length of time the blood cell analyzer is put to sleep includes: setting the temperature of the preheating pool to a first temperature when the blood cell analyzer is just put into sleep; setting the temperature of the preheating tank to a second temperature when the time period for which the blood cell analyzer is put to sleep reaches a first time period, wherein the second temperature is lower than the first temperature; and setting the temperature of the pre-heating tank to a third temperature when a period of time for which the blood cell analyzer is put to sleep reaches a second period of time, the second period of time being greater than the first period of time, the third temperature being lower than the second temperature.

In one embodiment of the present application, the analysis method further comprises: timing a time when the blood cell analyzer enters the sleep after the blood cell analyzer enters the sleep, and discharging bubbles in the pre-heat tank at predetermined time intervals based on the timing.

In one embodiment of the present application, the analysis method further comprises: the air bubbles in the preheated cell of the hematology analyzer are discharged before each time the hematology analyzer performs white blood cell counting and sorting.

In another aspect of the present application, there is provided a blood cell analyzer including: the sampling assembly is used for feeding the collected blood sample into the counting cell; the preheating tank is used for heating the diluent before entering the counting tank; a hemolytic agent supply assembly for supplying hemolytic agent into the counting cell; the counting cell is used for performing hemolysis treatment on the diluted blood and counting and classifying leucocytes; and the bubble discharging device is used for discharging bubbles in the preheating pool after the counting pool is out of dormancy, and the diluent discharged from the bubbles is used for counting and classifying the white blood cells.

In one embodiment of the present application, the bubble discharging device is further configured to deliver the diluent to the preheating tank and the counting tank.

In one embodiment of the present application, the bubble discharge device is an injector that discharges bubbles in the preheating tank by sucking out a part of the diluent in the preheating tank and supplying new diluent to the preheating tank to refill the preheating tank.

In one embodiment of the present application, the blood cell analyzer further comprises a first valve, a second valve, and a diluent barrel that provides a diluent, wherein: the injector sucks part of the diluent out of the preheating tank based on the communication between the first valve and the second valve and the communication between the second valve and the preheating tank so as to empty the top of the preheating tank; the injector discharges the diluent sucked out of the preheating tank through the first pipeline based on the communication between the first valve and the first pipeline; the injector draws new diluent from the diluent barrel for replenishment of the pre-heat tank with new diluent based on the communication of the first valve with the second valve and the communication of the second valve with the diluent barrel; the injector replenishes the preheating tank with new diluent drawn out of the diluent bucket based on the communication between the first valve and the second valve and the communication between the second valve and the preheating tank to refill the preheating tank.

In one embodiment of the present application, the blood cell analyzer further comprises: and the preheating pool temperature adjusting device is used for reducing the temperature of the preheating pool after the counting pool enters the dormancy again.

In one embodiment of the present application, the blood cell analyzer further comprises: the dormancy time timing device is used for timing the time when the counting pool enters the dormancy again; and the preheating tank temperature adjusting device is used for adjusting the temperature of the preheating tank based on the length of time for the counting tank to enter the dormancy state so as to reduce the amount of gas separated out from the diluent in the preheating tank during the dormancy state of the counting tank.

In one embodiment of the present application, the adjusting of the temperature of the preheating bath by the preheating bath temperature adjusting device includes: gradually reducing the temperature of the preheating pool as the time for the counting pool to enter the sleep state becomes longer.

In one embodiment of the present application, the adjusting of the temperature of the preheating bath by the preheating bath temperature adjusting device includes: setting the temperature of the preheating pool to be a first temperature when the counting pool just enters the dormancy state; when the time period that the counting pool enters the dormancy reaches a first time period, setting the temperature of the preheating pool to be a second temperature, wherein the second temperature is lower than the first temperature; and when the time period that the counting pool enters the dormancy state reaches a second time period, setting the temperature of the preheating pool to be a third temperature, wherein the second time period is greater than the first time period, and the third temperature is lower than the second temperature.

In one embodiment of the present application, the blood cell analyzer further comprises: the dormancy time timing device is used for timing the time of the counting pool entering dormancy after the counting pool enters dormancy; and the bubble removal device is further configured to: after the counting cell enters the sleep mode, the bubbles in the preheating cell are discharged at preset time intervals based on the timing of the sleep time timing device.

In one embodiment of the present application, the bubble removal device is further configured to: and before each time of counting and classifying the white blood cells in the counting pool, discharging the bubbles in the preheating pool.

According to the analysis method of the blood cell analyzer and the blood cell analyzer, the bubbles in the preheating pool are discharged after the blood cell analyzer is out of dormancy, so that the blood cell analyzer is based on the diluent with bubbles removed when the blood cell analyzer is used for counting and classifying the white blood cells, the white blood cell counting and classifying result cannot be influenced due to the problem of bubbles, and the reliability of the blood analysis result is improved.

Drawings

FIG. 1 shows a schematic flow diagram of an analysis method of a blood cell analyzer according to one embodiment of the present application;

fig. 2A to 2E are schematic views showing a process of discharging bubbles in the pre-heat bath in the analysis method of the blood cell analyzer according to the embodiment of the present application;

FIG. 3 is a schematic flow chart diagram showing an analysis method of a blood cell analyzer according to another embodiment of the present application;

FIG. 4 is a schematic flow chart showing an analysis method of a blood cell analyzer according to still another embodiment of the present application;

FIG. 5 is a schematic flow chart diagram showing an analysis method of a blood cell analyzer according to still another embodiment of the present application;

FIG. 6 shows a schematic block diagram of a blood cell analyzer according to one embodiment of the present application;

FIG. 7 shows a schematic block diagram of a blood cell analyzer according to another embodiment of the present application;

FIG. 8 shows a schematic block diagram of a blood cell analyzer according to yet another embodiment of the present application; and

fig. 9 shows a schematic block diagram of a blood cell analyzer according to yet another embodiment of the present application.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, exemplary embodiments according to the present application will be described in detail below with reference to the accompanying drawings. It should be understood that the described embodiments are only some embodiments of the present application and not all embodiments of the present application, and that the present application is not limited by the example embodiments described herein. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the application described in the application without inventive step, shall fall within the scope of protection of the application.

In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present application. It will be apparent, however, to one skilled in the art, that the present application may be practiced without one or more of these specific details. In other instances, well-known features of the art have not been described in order to avoid obscuring the present application.

It is to be understood that the present application is capable of implementation in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the application to those skilled in the art.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. As used herein, the term "and/or" includes any and all combinations of the associated listed items.

In order to provide a thorough understanding of the present application, detailed steps and detailed structures will be provided in the following description in order to explain the technical solutions proposed in the present application. The following detailed description of the preferred embodiments of the present application, however, will suggest that the present application may have other embodiments in addition to these detailed descriptions.

First, the background of the invention of the present application is described in general. The blood cell analyzer in the embodiment of the application comprises a preheating pool and a counting pool, wherein the preheating pool heats diluent to a certain temperature, the heated diluent is sent into the counting pool to dilute a blood sample, and the counting pool is used for performing hemolysis treatment on the diluted blood and counting and classifying leucocytes. Better counting and sorting results can be obtained by counting and sorting leukocytes based on blood diluted with heated diluent. Especially for animal blood cell analysis, heating the diluent is the key to realize four-classification and five-classification of animal white blood cells. The blood cell analyzer in the present application is preferably an animal blood cell analyzer. However, this introduces a new technical problem that the diluent is subjected to a temperature difference change during the heating process by the preheating well, so that the gas dissolved in the diluent is separated out and exists in the preheating well in the form of bubbles, and the separated gas amount is increased with the increase of the resting time of the blood cell analyzer (counting well), which causes the instability of the blood cell analyzer in the quantitative processes of sample collection, reagent addition, etc., thereby affecting the measurement result, as described above. Based on the finding of this technical problem, the present application provides an analysis method of a blood cell analyzer to solve the technical problem as will be described in detail below.

Next, an analysis method 100 of a blood cell analyzer according to an embodiment of the present application is first described with reference to fig. 1. As shown in fig. 1, the analysis method 100 of the blood cell analyzer may include the steps of:

in step S110, after the blood cell analyzer exits from the sleep, air bubbles in the preheated bath of the blood cell analyzer are discharged.

In step S120, the diluent in the preheated cell discharged with air bubbles is sent to a counting cell of the blood cell analyzer for counting and classifying white blood cells.

In an embodiment of the present application, the blood cell analyzer may comprise a process of going out of sleep, starting operation, and going into sleep, which is determined according to the actual requirement of blood analysis, because of uncertainty of the arrival of blood samples, the blood cell analyzer may go into a sleep state after completing the analysis of one batch of samples, and then operate after going out of the sleep state when the next batch of samples arrives. After the blood cell analyzer enters the dormant state from the working state, the preheating tank still continues to heat the diluent to prepare for analyzing a next batch of samples, so that in the dormant process of the blood cell analyzer, gas separated out from the diluent in the preheating tank increases along with the increase of the dormant time. Based on this, in the analysis method 100 of the blood cell analyzer according to the embodiment of the present application, after the blood cell analyzer is taken out of the sleep mode, the air bubbles in the pre-heated cell of the blood cell analyzer may be discharged (as set forth in step S110), and then the white blood cells may be counted and sorted based on the diluted liquid discharged with the air bubbles (as set forth in step S120). Therefore, the influence on the counting and classifying result of the white blood cells caused by the problem of bubbles is avoided, and the reliability of the blood analysis result is improved.

In an embodiment of the present application, the process of discharging bubbles in the preheating bath in step S110 may include: sucking out a part of the diluent in the preheating tank to enable the bubbles to be retained at the top of the preheating tank which is emptied; and supplementing new diluent into the preheating pool to refill the preheating pool and discharge the gas at the top of the preheating pool. In this embodiment, the bubbles at the top of the diluent are separated from the diluent by sucking part of the diluent out of the preheating tank into a cavity at the top of the preheating tank resulting from the sucking of part of the diluent; after gas-liquid separation, new diluent is supplemented into the preheating tank, so that the preheating tank is refilled with the diluent, and gas in a cavity at the top of the preheating tank is discharged along with the supplement of the new diluent, thereby realizing the discharge of bubbles in the preheating tank.

Further, starting from the specific structure of the blood cell analyzer, the automatic discharge of the bubbles in the preheating tank can be realized by the following processes: the injector of the blood cell analyzer is communicated with the preheating pool, and part of the diluent is sucked out of the preheating pool by the injector so that the top of the preheating pool is emptied; the injector discharges the diluent sucked out of the preheating pool through a first pipeline; the injector is communicated with the diluent barrel, and new diluent is sucked out of the diluent barrel by the injector to be used for supplementing new diluent to the preheating pool; and the injector is communicated with the preheating tank, and new diluent sucked out from the diluent barrel is replenished into the preheating tank by the injector so as to refill the preheating tank.

In order to make the above description clearer, the process of discharging bubbles in the preheating bath is described below with reference to fig. 2A to 2E.

First, fig. 2A shows an initial state of the blood cell analyzer, i.e., a state of the blood cell analyzer after exiting from sleep. As shown in FIG. 2A, in the initial state, bubbles are accumulated at the top of the preheating tank, and the diluent is not delivered to the counting tank. Wherein, the diluent in the preheating tank is delivered to the preheating tank by an injector after the diluent is pumped out from the diluent barrel. The syringe is connected with a first valve SV1, and the preheating pool and the diluent barrel are both connected with a second valve SV 2. The first valve SV1 includes terminals a1, c1 and d1, and the second valve SV2 includes terminals a2, c2 and d 2. When the terminals a1 and d1 are connected and the terminals a2 and c2 are connected, the injector is communicated with the preheating pool; when the terminals a1 and d1 are connected and the terminals a2 and d2 are connected, the syringe is communicated with the diluent barrel; when the terminals a1 and c1 are connected, the syringe communicates with the first line T1. The first line T1 may be a line for discharging liquid in the annotator. Furthermore, the preheating bath is also connected to a third valve SV3, which third valve SV3 comprises terminals a3, c3 and d 3. When the terminals a3 and d3 are connected, the preheating tank is communicated with the second pipeline T2, so that the diluent in the preheating tank is conveyed to the counting tank through the second pipeline T2; when the terminals a3 and c3 are connected, the preheat tank communicates with the third line T3, thereby delivering diluent to the bottom of the count tank via the third line T3 and out of the count tank.

FIG. 2B shows a schematic diagram of a syringe of the hematology analyzer aspirating a portion of the diluent from the pre-heated reservoir. As shown in fig. 2B, the terminal a1 of the first valve SV1 connects the terminal d1, and the terminal a2 of the second valve SV2 connects the terminal c2, thereby allowing the syringe to communicate with the preheating bath. Based on this communication, the injector may draw a portion of the diluent from the pre-heat well such that the top of the pre-heat well is emptied and the fluid path flows as indicated by the arrows in fig. 2B.

Fig. 2C shows a schematic diagram of the syringe of the blood cell analyzer discharging the diluent sucked out of the pre-heating well through the first line. As shown in fig. 2C, terminal a1 of the first valve SV1 connects terminal C1 so that the syringe communicates with the first line T1. Based on this communication, the injector can discharge the diluent drawn from the pre-heat tank through a first line T1, the flow of which is indicated by the arrow in fig. 2C.

Fig. 2D shows a schematic of a syringe of the hematology analyzer drawing new diluent from the diluent barrel for replenishment into the pre-heated cell. As shown in fig. 2D, terminal a1 of the first valve SV1 connects to terminal D1, and terminal a2 of the second valve SV2 connects to terminal D2, thereby putting the syringe in communication with the diluent barrel. Based on this communication, the syringe may draw new diluent from the diluent drum for replenishment into the pre-heat tank, with the flow path flowing as indicated by the arrows in fig. 2D.

Fig. 2E shows a schematic of the syringe of the hematology analyzer filling the pre-heated cell with new diluent drawn from the diluent barrel. As shown in fig. 2E, the terminal a1 of the first valve SV1 connects the terminal d1, and the terminal a2 of the second valve SV2 connects the terminal c2, thereby allowing the syringe to communicate with the preheating bath. Based on the communication, the injector can feed new diluent sucked from the diluent barrel into the preheating tank, so that the preheating tank is refilled with the diluent, and thus, the process of discharging bubbles in the preheating tank is realized, and the liquid path flows as shown by an arrow in fig. 2E.

While an exemplary process of discharging bubbles in the preheat tank according to an embodiment of the present invention is described above with reference to fig. 2A through 2E, it is to be understood that the amount of diluent drawn out of the preheat tank by the syringe may depend on the length of the sleep time of the count tank. As mentioned above, the longer the sleep time of the counting cell is, the more gas is separated out from the preheating cell, so that the diluent sucked out from the preheating cell by the injector can be relatively more; the shorter the sleep time of the counting cell, the less gas is evolved in the preheating cell, and thus the diluent drawn out of the preheating cell by the injector can be relatively less. Based on the guiding idea, the specific amount of the diluent sucked out from the preheating tank by the injector can be set empirically or calculated according to a certain calculation method, which is not limited in this application. Further, it should be understood that the process of discharging the bubbles in the preheating tank according to the embodiment of the present invention may be any other suitable process, and is not limited to the above process.

Based on the diluent discharged from the bubble, the counting and classifying of the white blood cells performed by the counting cell can avoid the influence on the counting and classifying result of the white blood cells caused by the bubble problem, and the reliability of the blood analysis result is improved.

A schematic flow chart of an analysis method 300 of a blood cell analyzer according to another embodiment of the present application is described below with reference to fig. 3. As shown in fig. 3, the analysis method 300 of the blood cell analyzer may include the steps of:

in step S310, after the blood cell analyzer exits from the sleep, air bubbles in the preheated bath of the blood cell analyzer are discharged.

In step S320, the diluent in the preheated cell discharged with air bubbles is sent to a counting cell of the blood cell analyzer for counting and classifying white blood cells.

In step S330, after the blood cell analyzer is put to sleep again, the temperature of the pre-heating bath is lowered.

Step S310 and step S320 are similar to step S110 and step S120 in the method 100 described above with reference to fig. 1, respectively, and are not described herein again for brevity. In contrast to the method 100 depicted in fig. 1, the analysis method 300 of the blood cell analyzer further includes a step S330, which depicts the operation of the (cell of the) blood cell analyzer after it has been put back into sleep. It should be understood that after the blood cell analyzer has processed a batch of samples, a timer may be timed and the resting state may be re-entered after a certain time has elapsed. In step S330, after the blood cell analyzer enters the sleep again, the temperature of the preheating tank can be lowered, so as to reduce the amount of gas separated out by the blood cell analyzer during the sleep process, thereby reducing the amount of bubbles that need to be discharged from the preheating tank after the blood cell analyzer exits the sleep next time, i.e., reducing the diluent that needs to be sucked out from the preheating tank after the blood cell analyzer exits the sleep next time, thereby reducing the diluent that needs to be replenished into the preheating tank, and further saving the cost while improving the reliability of the blood analysis.

A schematic flow chart of an analysis method 400 of a blood cell analyzer according to still another embodiment of the present application is described below with reference to fig. 4. As shown in fig. 4, the analysis method 400 of the blood cell analyzer may include the steps of:

in step S410, after the blood cell analyzer exits from the sleep, air bubbles in the preheated cell of the blood cell analyzer are discharged.

In step S420, the diluent in the preheated cell discharged with air bubbles is sent to a counting cell of the blood cell analyzer for counting and classifying white blood cells.

In step S430, after the blood cell analyzer is put into sleep again, the time when the blood cell analyzer is put into sleep is counted.

In step S440, the temperature of the pre-heat tank is adjusted based on the length of time the blood cell analyzer is put to sleep, so as to reduce the amount of gas evolved from the diluent in the pre-heat tank during the sleep of the blood cell analyzer.

Step S410 and step S420 are similar to step S110 and step S120 in the method 100 described above with reference to fig. 1, respectively, and are not described herein again for brevity. Unlike the method 100 depicted in fig. 1, the analysis method 400 of the blood cell analyzer further includes steps S430 and S440, which describe the operation of the blood cell analyzer after it has been put back into sleep. As described in steps S430 and S440, after the blood cell analyzer enters the sleep again, the time for the blood cell analyzer to enter the sleep may be counted, and the temperature of the pre-heat tank may be adjusted based on the counted time (i.e., the length of time for the blood cell analyzer to enter the sleep) to reduce the amount of gas released from the diluent in the pre-heat tank during the sleep period of the blood cell analyzer. In this embodiment, the temperature of the preheating tank is adjusted based on the length of time for which the blood cell analyzer is put to sleep, and when the time for which the blood cell analyzer is put to sleep is shorter, the temperature of the preheating tank can be made higher (for example, the temperature is adjusted less than when just put to sleep); when the time for which the blood cell analyzer enters the sleep state is longer, the temperature of the preheating tank can be slightly lower (for example, the temperature is adjusted to be more than that when the blood cell analyzer just enters the sleep state), so that the time for heating the preheating tank (reheating to the required reaction temperature) after the blood cell analyzer exits the sleep state next time can be reduced while the gas amount separated out by the blood cell analyzer in the sleep state is reduced, and the processing efficiency of the blood cell analyzer is improved.

Illustratively, adjusting the temperature of the pre-heat bath based on the length of time the blood cell analyzer is put to sleep in step S440 may further include: gradually reducing the temperature of the pre-heating tank as the time for which the blood cell analyzer is put to sleep becomes longer. Further, for example, when the counting chamber is operated, the temperature of the preheating chamber is 42 ℃, and the temperature of the preheating chamber may be set to a first temperature (for example, 40 ℃) immediately after the blood cell analyzer is put to a sleep mode; setting the temperature of the pre-heating bath to a second temperature (e.g., 38 ℃) which is lower than the first temperature when the period in which the blood cell analyzer is put to sleep reaches a first period; and setting the temperature of the pre-heating bath to a third temperature (e.g., 35 ℃) when the period in which the blood cell analyzer is put to sleep reaches a second period, the second period being greater than the first period, the third temperature being lower than the second temperature. The above examples can both reduce the amount of gas evolved by the blood cell analyzer during sleep and reduce the time for heating the preheating tank after the blood cell analyzer exits sleep next time, thereby improving the processing efficiency of the blood cell analyzer. It should be understood that the aforementioned "first temperature", "second temperature", "third temperature", "first time period" and "second time period" can be set arbitrarily according to the requirement, and are not limited to include the above-mentioned temperatures and time periods, but can include more or less temperatures/time periods, and can be adjusted arbitrarily according to the requirement.

A schematic flow chart of an analysis method 500 of a blood cell analyzer according to still another embodiment of the present application is described below with reference to fig. 5. As shown in fig. 5, the analyzing method 500 of the blood cell analyzer may include the steps of:

in step S510, after the blood cell analyzer exits from the sleep mode, air bubbles in the preheated cell of the blood cell analyzer are discharged.

In step S520, the diluent in the preheated cell discharged with air bubbles is sent to a counting cell of the blood cell analyzer for counting and classifying white blood cells.

In step S530, after the blood cell analyzer goes to sleep, the time at which the blood cell analyzer goes to sleep is counted, and bubbles in the pre-heat bath are discharged at predetermined time intervals based on the counting.

Step S510 and step S520 are similar to step S110 and step S120 in the method 100 described above with reference to fig. 1, respectively, and are not described herein again for brevity. Unlike the method 100 depicted in fig. 1, the analysis method 500 of the blood cell analyzer further includes a step S530, which describes the operation after (the counting cell of) the blood cell analyzer enters the sleep mode. After the blood cell analyzer enters the sleep mode, the time for the blood cell analyzer to enter the sleep mode may be counted, and the air bubbles in the pre-heat tank may be discharged based on the timing, as described in step S530. In this embodiment, the bubbles in the preheating tank are discharged at regular intervals, rather than being discharged after the next sleep exit, and therefore, the method is simple in logic, easy to implement, and it is possible to realize that the bubbles in the preheating tank are discharged when the sleep exit is performed next time, and thus, the processing efficiency of the blood cell analyzer can be further improved while the reliability of the blood cell analysis is improved. It should be understood that step S530 may not need to be sequenced after steps S510 and S520, and step S530 may constitute a separate scheme for implementing the bubble discharge of the preheating tank. In addition, step S530 may also be combined with the method described above with reference to fig. 3 and fig. 4, and is not described herein again for brevity.

In a further embodiment of the present application, the analysis method of the blood cell analyzer may further include (not shown in the drawings): the air bubbles in the preheated cell of the hematology analyzer are discharged before each time the hematology analyzer performs white blood cell counting and sorting. In this embodiment, the air bubbles in the pre-heated cell of the hematology analyzer may be vented out of the way before each white blood cell count and classification, regardless of whether the hematology analyzer is going out of sleep or into sleep. For example, after a batch of samples are processed by a general blood cell analyzer, timing may be performed, and the sleep state is only entered after the timing reaches a certain time.

The analysis method of the blood cell analyzer according to the embodiment of the present invention is exemplarily shown above. Based on the above description, the analysis method of the blood cell analyzer according to the embodiment of the present invention enables the blood cell analyzer to be based on the diluent from which air bubbles have been removed when performing white blood cell counting and sorting by discharging the air bubbles in the preheating tank after the blood cell analyzer is out of sleep, so that the white blood cell counting and sorting results are not affected by the air bubbles, and the reliability of the blood analysis results is improved. In addition, according to the analysis method of the blood cell analyzer provided by the embodiment of the invention, the temperature of the preheating pool is reduced after the blood cell analyzer enters the dormancy again, so that the cost can be saved while the reliability of blood analysis is improved. In addition, according to the analysis method of the blood cell analyzer of the embodiment of the invention, the time for the blood cell analyzer to enter the sleep mode is measured after the blood cell analyzer enters the sleep mode again, and the temperature of the preheating tank is adjusted based on the measured time, so that the gas quantity separated out in the sleep mode of the blood cell analyzer is reduced, the time for heating the preheating tank after the blood cell analyzer exits the sleep mode next time is also reduced, and the processing efficiency of the blood cell analyzer is improved. Further, according to the analysis method of the blood cell analyzer of the embodiment of the invention, before the blood cell analyzer counts and classifies white blood cells each time, air bubbles in the preheating tank of the blood cell analyzer are discharged, so that the processing precision of the blood cell analyzer can be further improved.

A blood cell analyzer provided according to another aspect of the present application is described below with reference to fig. 6. Fig. 6 shows a schematic block diagram of a blood cell analyzer 600 according to an embodiment of the present application. As shown in fig. 6, the blood cell analyzer 600 includes a sampling assembly 610, a preheating reservoir 620, a hemolytic agent supply assembly 630, a counting reservoir 640, and an air bubble removal device 650. Wherein the sampling assembly 610 is adapted to deliver a collected blood sample to the counting chamber 640. The preheating tank 620 is used for heating the diluent before entering the counting tank 640. The hemolytic agent supply component 630 is used for supplying hemolytic agent into the counting chamber 640. The counting chamber 640 is used for performing hemolysis treatment on the diluted blood, and counting and classifying leukocytes. The bubble discharging device 650 is used for discharging bubbles in the preheating tank 620 after the counting tank 640 exits from the sleep mode, so that the counting tank 640 can count and classify the white blood cells based on the discharged bubble diluent. The blood cell analyzer 600 can implement the analysis method 100 of the blood cell analyzer described above in connection with fig. 1.

In an embodiment of the present application, the blood cell analyzer 600 may comprise a process of going out of sleep, starting operation, and going into sleep, which is determined according to the actual requirement of blood analysis, because of uncertainty of the arrival of blood samples, the blood cell analyzer 600 (the counting cell 640 thereof) may go into a sleep state after completing the analysis of one batch of samples, and then go out of the sleep state to operate until the next batch of samples comes. Since the preheating tank 620 continues to heat the diluent to prepare for the next sample analysis after the blood cell analyzer 600 enters the resting state from the operating state, the gas evolved in the diluent in the preheating tank 620 increases with the increase of the resting time during the resting process of the counting tank 640. Based on this, in the blood cell analyzer 600 according to the embodiment of the present application, after the counting chamber 640 exits from the sleep mode, the bubble discharging device 650 may discharge bubbles in the preheating chamber 620, and then the counting chamber 640 performs white blood cell counting and sorting based on the diluted solution discharged with bubbles. Therefore, the influence on the counting and classifying result of the white blood cells caused by the problem of bubbles is avoided, and the reliability of the blood analysis result is improved.

In embodiments of the present application, the air bubble removal device 650 may also be used to deliver dilution liquid to the preheat bath 620 and the count bath 640. That is, the air bubble discharging device 650 may be an injector that can discharge air bubbles in the preheating bath 620 by sucking out a portion of the diluent in the preheating bath 620 and supplying new diluent to the preheating bath 620 to refill the preheating bath 620. In this embodiment, the gas bubbles at the top of the diluent are separated from the diluent by sucking a portion of the diluent out of the pre-heat bath 620 through a syringe, to a cavity at the top of the pre-heat bath 620 resulting from the sucking of the portion of the diluent; after gas-liquid separation, the injector replenishes new diluent to the preheating tank 620, so that the preheating tank 620 is refilled with the diluent, and the gas in the cavity at the top of the preheating tank 620 is discharged along with the replenishment of the new diluent, thereby realizing the discharge of bubbles in the preheating tank 620.

Further, the blood cell analyzer 600 may further include a first valve, a second valve, and a diluent tank (all not shown in fig. 6, see fig. 2A to 2E) that provides diluent. To achieve the discharge of bubbles in the pre-heat bath 620, first, the injector may suck a part of the diluent out of the pre-heat bath 620 based on the communication between the first valve and the second valve and the communication between the second valve and the pre-heat bath 620 to empty the top of the pre-heat bath 620; then, the injector may discharge the diluent sucked from the preheating tank 620 through the first pipe based on the communication of the first valve with the first pipe; then, the injector may draw new diluent from the diluent barrel based on the communication of the first valve with the second valve and the communication of the second valve with the diluent barrel for replenishing the preheat tank 620 with new diluent; finally, the injector may replenish the pre-heat well 620 with new diluent drawn from the diluent barrel based on the communication of the first valve with the second valve and the communication of the second valve with the pre-heat well 620 to refill the pre-heat well 620. The structure and operation of the bubble removing device 650 of the blood cell analyzer 600 can be understood with reference to fig. 2A to 2E in conjunction with the foregoing description, and for brevity, will not be described again.

It should be appreciated that the amount of diluent drawn from the pre-heat well 620 by the syringe may depend on the length of the resting time of the counter 640. As mentioned above, the longer the sleep time of the counting cell 640 is, the more gas is separated out from the preheating cell 620, and thus the more diluent the injector can suck out from the preheating cell 620; the shorter the sleep time of the counting cell 640, the less gas is evolved in the preheating cell 620, and thus the diluent drawn out of the preheating cell 620 by the injector can be relatively less. Based on this guiding concept, the specific amount of the diluent sucked out from the preheating tank 620 by the injector may be set empirically or calculated according to a certain calculation method, which is not limited in this application. Further, it should be understood that the bubble discharging device 650 according to the embodiment of the present invention may be any other suitable structure, and the bubble discharging process may be any other suitable process, and is not limited to the above structure and process.

Based on the diluted solution discharged from the bubble, the counting and classifying of the white blood cells performed by the counting cell 640 can avoid the influence on the counting and classifying result of the white blood cells caused by the bubble problem, thereby improving the reliability of the blood analysis result.

A schematic block diagram of a blood cell analyzer 700 according to another embodiment of the present application is described below with reference to fig. 7. As shown in fig. 7, the blood cell analyzer 700 includes a sampling assembly 710, a pre-heat bath 720, a hemolytic agent supply assembly 730, a counting bath 740, an air bubble exhausting device 750, and a pre-heat bath temperature adjusting device 760. Wherein the sampling assembly 710 is adapted to deliver a collected blood sample to the counting cell 740. The pre-heating tank 720 is used to heat the diluent before entering the counting tank 740. The hemolytic agent supply assembly 730 is used for supplying hemolytic agent into the counting chamber 740. The counting chamber 740 is used for performing hemolysis treatment on the diluted blood, and counting and classifying leukocytes. The bubble discharging device 750 is used for discharging bubbles in the preheating tank 720 after the counting tank 740 goes out of the sleep mode, so that the counting tank 740 counts and classifies the white blood cells based on the discharged bubble diluent. The preheat bath temperature adjustment 760 is used to lower the temperature of the preheat bath 720 after the count bath 740 has re-entered sleep. The blood cell analyzer 700 can implement the analysis method 300 of the blood cell analyzer described above in connection with fig. 3.

The sampling assembly 710, the preheating tank 720, the hemolytic agent supply assembly 730, the counting tank 740, and the air bubble removal device 750 are similar to the sampling assembly 610, the preheating tank 620, the hemolytic agent supply assembly 630, the counting tank 640, and the air bubble removal device 650 described above with reference to fig. 6, respectively, and therefore, for brevity, no further description is provided herein. Unlike the blood cell analyzer 600 depicted in fig. 6, the blood cell analyzer 700 further includes a pre-heat bath temperature adjustment apparatus 760. After the counting cell 740 enters the sleep again, the preheating cell temperature adjusting device 760 can reduce the temperature of the preheating cell 720, so as to reduce the gas amount separated out from the counting cell 740 in the sleep process, thereby reducing the amount of gas bubbles required to be discharged from the preheating cell 720 after the counting cell 740 exits the sleep next time, i.e., reducing the diluent required to be sucked out from the preheating cell 720 after the counting cell exits the sleep next time, thereby reducing the diluent required to be supplemented into the preheating cell 720, and further saving the cost while improving the reliability of blood analysis.

A schematic block diagram of a blood cell analyzer 800 according to yet another embodiment of the present application is described below with reference to fig. 8. As shown in fig. 8, the blood cell analyzer 800 includes a sampling assembly 810, a pre-heat bath 820, a hemolytic agent supply assembly 830, a counting bath 840, an air bubble exhausting device 850, a pre-heat bath temperature adjusting device 860, and a sleep time timing device 870. Wherein the sampling assembly 810 is adapted to deliver a collected blood sample to the counting cell 840. The preheating tank 820 is used for heating the diluent before entering the counting tank 840. The hemolytic agent supply assembly 830 is used for supplying hemolytic agent into the counting chamber 840. The counting cell 840 is used for performing hemolysis treatment on the diluted blood, and counting and classifying leukocytes. The bubble discharging device 850 is used for discharging bubbles in the preheating tank 820 after the counting tank 840 exits from the sleep mode, so that the counting tank 840 counts and classifies white blood cells based on the discharged bubble diluent. The sleep time timing device 870 is used to time the time when the counting pool 840 enters into sleep after the counting pool 840 enters into sleep again. The preheating tank temperature adjusting device 860 is used for adjusting the temperature of the preheating tank 820 based on the length of time for which the counting tank 840 goes to sleep so as to reduce the amount of gas separated out by the diluent in the preheating tank 820 during the sleep period of the counting tank 840. The blood cell analyzer 800 can implement the analysis method 400 of the blood cell analyzer described above in connection with fig. 4.

The sampling assembly 810, the preheating tank 820, the hemolytic agent supply assembly 830, the counting tank 840 and the air bubble exhausting device 850 are similar to the sampling assembly 610, the preheating tank 620, the hemolytic agent supply assembly 630, the counting tank 640 and the air bubble exhausting device 650, respectively, which are described above with reference to fig. 6, and therefore, for brevity, no further description is provided here. Unlike the blood cell analyzer 600 depicted in fig. 6, the blood cell analyzer 800 further includes a pre-heat cell temperature adjustment device 860 and a sleep time timing device 870. After the counting cell 840 enters the sleep again, the sleep time timing device 870 may time the time when the counting cell 840 enters the sleep, and the preheating cell temperature adjusting device 860 may adjust the temperature of the preheating cell 820 based on the time (i.e., the length of the time when the counting cell 840 enters the sleep) to reduce the amount of gas released from the diluent in the preheating cell 820 during the sleep period of the counting cell 840. In this embodiment, preheat pool temperature adjustment device 860 adjusts the temperature of preheat pool 820 based on the length of time that count pool 840 enters sleep, which may cause the temperature of preheat pool 820 to be higher (e.g., lower than when it just entered sleep) when the length of time that count pool 840 enters sleep is shorter; when the time for which the counting cell 840 enters the sleep mode is longer, the temperature of the preheating cell 820 can be made lower (for example, the temperature is adjusted to be lower than that when the counting cell 840 just enters the sleep mode), so that the time for heating the preheating cell 820 (reheating to the required reaction temperature) after the counting cell 840 exits the sleep mode next time can be reduced while the amount of gas evolved during the sleep mode is reduced, and the processing efficiency of the blood cell analyzer 800 can be improved.

Illustratively, the warming pool temperature adjusting device 860 adjusting the temperature of the warming pool 820 based on the length of time the count pool 840 goes to sleep may further include: the temperature of the preheat tank 820 is gradually decreased as the time for the count tank 840 to go to sleep becomes longer. Further, for example, when the counting cell 840 is in operation, the preheating cell temperature is 42 ℃, and the temperature of the preheating cell 820 may be set to a first temperature (e.g., 40 ℃) immediately after the counting cell 840 enters the sleep mode; setting the temperature of the preheating bath 820 to a second temperature (e.g., 38 ℃) which is lower than the first temperature, when the period of time in which the counting bath 840 goes to sleep reaches a first period of time; and setting the temperature of the preheating bath 820 to a third temperature (e.g., 35 ℃) when the period of time during which the count bath 840 goes to sleep reaches a second period of time, the second period of time being greater than the first period of time, the third temperature being lower than the second temperature. The above examples can both reduce the amount of gas evolved by the counting cell 840 during sleep and reduce the time required for heating the pre-heating cell 820 after exiting sleep next time, thereby improving the processing efficiency of the blood cell analyzer. It should be understood that the aforementioned "first temperature", "second temperature", "third temperature", "first time period" and "second time period" can be set arbitrarily according to the requirement, and are not limited to include the above-mentioned temperatures and time periods, but can include more or less temperatures/time periods, and can be adjusted arbitrarily according to the requirement.

A schematic block diagram of a blood cell analyzer 900 according to yet another embodiment of the present application is described below with reference to fig. 9. As shown in fig. 9, the blood cell analyzer 900 includes a sampling assembly 910, a pre-heat cell 920, a hemolytic agent supply assembly 930, a counting cell 940, a bubble removal device 950, and a sleep time timing device 970. Wherein the sampling assembly 910 is adapted to deliver a collected blood sample to the counting cell 940. The preheating tank 920 is used for heating the diluent before entering the counting tank 940. The hemolytic agent supply component 930 is used to supply hemolytic agent into the counting cell 940. The counting cell 940 is used for performing hemolysis treatment on the diluted blood, and counting and classifying leukocytes. The bubble discharging device 950 is used for discharging bubbles in the preheating tank 920 after the counting cell 940 is out of dormancy, so that the counting cell 940 counts and classifies white blood cells based on the discharged bubble diluent. The sleep time timing means 970 is used for timing the time when the counting pool 940 goes to sleep after the counting pool 940 goes to sleep. And, the bubble exhausting means 950 is further configured to exhaust the bubbles in the preheating tank 920 at predetermined time intervals based on the timing of the sleep time timing means 970 after the counting tank 940 enters the sleep mode. The blood cell analyzer 900 can implement the analysis method 500 of the blood cell analyzer described above in connection with fig. 5.

The sampling assembly 910, the preheating tank 920, the hemolytic agent supply assembly 930, the counting tank 940, and the air bubble exhausting device 950 are similar to the sampling assembly 610, the preheating tank 620, the hemolytic agent supply assembly 630, the counting tank 640, and the air bubble exhausting device 650 described above with reference to fig. 6, respectively, and therefore, for brevity, no further description is provided herein. Unlike the blood cell analyzer 600 depicted in fig. 6, the blood cell analyzer 900 further includes a sleep time timing means 970. After the cell 940 enters the sleep mode, the time for the cell 940 to enter the sleep mode may be counted, and the bubbles in the preheating tank 920 may be discharged based on the timing. In this embodiment, the bubbles in the preheating bath 920 are discharged at regular intervals, rather than being discharged after the next sleep exit, and therefore, the logic is simple, the implementation is easy, and it is possible to realize that the bubbles in the preheating bath 920 are already discharged when the sleep exit is performed next time, and thus, the processing efficiency of the blood cell analyzer can be further improved while the reliability of the blood cell analysis is improved.

In a further embodiment of the present application, the aforementioned air bubble removing device of the blood cell analyzer can be further used for: the air bubbles in the preheating pool are discharged before each time the counting and classifying of the white blood cells are carried out in the counting pool. In this embodiment, the bubbles in the pre-heated cell may be vented out of the cell before each white blood cell count and classification, regardless of whether the cell is going out of sleep or into sleep. For example, after a batch of samples is processed by the counting cell, timing may be performed, and the counting cell may enter the sleep state only after the timing reaches a certain time.

The blood cell analyzer according to the embodiment of the present invention is exemplarily shown above. Based on the above description, the blood cell analyzer according to the embodiment of the present invention discharges air bubbles in the preheating tank after the blood cell analyzer exits from the sleep, so that the blood cell analyzer is based on the diluent from which the air bubbles have been removed when performing white blood cell counting and sorting, and therefore, the white blood cell counting and sorting results are not affected by the air bubbles, and the reliability of the blood analysis results is improved. In addition, the blood cell analyzer according to the embodiment of the invention reduces the temperature of the preheating tank after the blood cell analyzer enters the sleep again, so that the cost can be saved while the reliability of the blood analysis is improved. In addition, according to the blood cell analyzer of the embodiment of the invention, the time for the blood cell analyzer to enter the sleep mode is measured after the blood cell analyzer enters the sleep mode again, and the temperature of the preheating tank is adjusted based on the measured time, so that the amount of gas separated out in the sleep mode of the blood cell analyzer is reduced, the time for heating the preheating tank after the blood cell analyzer exits the sleep mode next time is reduced, and the processing efficiency of the blood cell analyzer is improved. Further, the blood cell analyzer according to the embodiment of the present invention discharges air bubbles in the preheating tank of the blood cell analyzer before the blood cell analyzer performs white blood cell counting and sorting each time, so that the processing accuracy of the blood cell analyzer can be further improved.

Although the example embodiments have been described herein with reference to the accompanying drawings, it is to be understood that the above-described example embodiments are merely illustrative and are not intended to limit the scope of the present application thereto. Various changes and modifications may be effected therein by one of ordinary skill in the pertinent art without departing from the scope or spirit of the present application. All such changes and modifications are intended to be included within the scope of the present application as claimed in the appended claims.

Those of ordinary skill in the art will appreciate that the various illustrative elements and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware or combinations of computer software and electronic hardware. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the implementation. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present application.

In the several embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other ways. For example, the above-described device embodiments are merely illustrative, and for example, the division of the units is only one logical functional division, and other divisions may be realized in practice, for example, a plurality of units or components may be combined or integrated into another device, or some features may be omitted, or not executed.

In the description provided herein, numerous specific details are set forth. However, it is understood that embodiments of the application may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.

Similarly, it should be appreciated that in the description of exemplary embodiments of the present application, various features of the present application are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the application and aiding in the understanding of one or more of the various inventive aspects. However, the method of the present application should not be construed to reflect the intent: this application is intended to cover such departures from the present disclosure as come within known or customary practice in the art to which this invention pertains. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this application.

It will be understood by those skilled in the art that all of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and all of the processes or elements of any method or apparatus so disclosed, may be combined in any combination, except combinations where such features are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, abstract and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise.

Furthermore, those skilled in the art will appreciate that while some embodiments described herein include some features included in other embodiments, rather than other features, combinations of features of different embodiments are meant to be within the scope of the application and form different embodiments. For example, in the claims, any of the claimed embodiments may be used in any combination.

The various component embodiments of the present application may be implemented in hardware, or in software modules running on one or more processors, or in a combination thereof. Those skilled in the art will appreciate that a microprocessor or Digital Signal Processor (DSP) may be used in practice to implement some or all of the functionality of some of the modules according to embodiments of the present application. The present application may also be embodied as apparatus programs (e.g., computer programs and computer program products) for performing a portion or all of the methods described herein. Such programs implementing the present application may be stored on a computer readable medium or may be in the form of one or more signals. Such a signal may be downloaded from an internet website or provided on a carrier signal or in any other form.

It should be noted that the above-mentioned embodiments illustrate rather than limit the application, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The application may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The usage of the words first, second and third, etcetera do not indicate any ordering. These words may be interpreted as names.

The above description is only for the specific embodiments of the present application or the description thereof, and the protection scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope disclosed in the present application, and shall be covered by the protection scope of the present application. The protection scope of the present application shall be subject to the protection scope of the claims.

Claims

An analysis method of a blood cell analyzer, comprising:

discharging air bubbles in a pre-heated cell of the blood cell analyzer after the blood cell analyzer exits from sleep; and

and sending the diluent in the preheated pool with the air bubbles discharged into a counting pool of the blood cell analyzer for counting and classifying the white blood cells.
The analysis method according to claim 1, wherein the bubble discharge process in the preheating tank comprises:

sucking out a part of the diluent in the preheating tank to enable the bubbles to be retained at the top of the preheating tank which is emptied; and

and supplementing new diluent into the preheating pool to refill the preheating pool and discharge the gas at the top of the preheating pool.
The analysis method according to claim 2, wherein the bubble discharge process in the preheating tank further comprises:

the injector of the blood cell analyzer is communicated with the preheating pool, and part of the diluent is sucked out of the preheating pool by the injector so that the top of the preheating pool is emptied;

the injector discharges the diluent sucked out of the preheating pool through a first pipeline;

the injector is communicated with the diluent barrel, and new diluent is sucked out of the diluent barrel by the injector to be used for supplementing new diluent to the preheating pool; and

the injector is communicated with the preheating tank, and new diluent sucked out of the diluent barrel is replenished into the preheating tank by the injector so as to refill the preheating tank.
The analytical method of any one of claims 1 to 3, further comprising:

after the blood cell analyzer is put to sleep again, the temperature of the preheating tank is lowered.
The analytical method of any one of claims 1 to 3, further comprising:

timing a time at which the blood cell analyzer enters the sleep state after the blood cell analyzer enters the sleep state again; and

adjusting the temperature of the pre-heat cell based on the length of time the blood cell analyzer is put to sleep to reduce the amount of gas evolved by the diluent in the pre-heat cell during sleep of the blood cell analyzer.
The method of claim 5, wherein adjusting the temperature of the pre-heat cell based on the length of time the blood cell analyzer is put to sleep comprises:

gradually reducing the temperature of the pre-heating tank as the time for which the blood cell analyzer is put to sleep becomes longer.
The method of claim 6, wherein adjusting the temperature of the pre-heat cell based on the length of time the blood cell analyzer is put to sleep comprises:

setting the temperature of the preheating pool to a first temperature when the blood cell analyzer is just put into sleep;

setting the temperature of the preheating tank to a second temperature when the time period for which the blood cell analyzer is put to sleep reaches a first time period, wherein the second temperature is lower than the first temperature; and

setting the temperature of the pre-heating tank to a third temperature when a period of time in which the blood cell analyzer is put to sleep reaches a second period of time, the second period of time being greater than the first period of time, the third temperature being lower than the second temperature.
The assay of any one of claims 1-3 and 5-7, further comprising:

timing a time when the blood cell analyzer enters the sleep after the blood cell analyzer enters the sleep, and discharging bubbles in the pre-heat tank at predetermined time intervals based on the timing.
The assay of any one of claims 1-3 and 5-7, further comprising:

the air bubbles in the preheated cell of the hematology analyzer are discharged before each time the hematology analyzer performs white blood cell counting and sorting.
A blood cell analyzer, comprising:

the sampling assembly is used for feeding the collected blood sample into the counting cell;

the preheating tank is used for heating the diluent before entering the counting tank;

a hemolytic agent supply assembly for supplying hemolytic agent into the counting cell;

the counting cell is used for performing hemolysis treatment on the diluted blood and counting and classifying leucocytes; and

and the bubble discharging device is used for discharging bubbles in the preheating pool after the counting pool is out of dormancy, and the diluent discharged from the bubbles is used for counting and classifying the white blood cells.
The blood cell analyzer of claim 10, wherein the bubble removal means is further configured to deliver the diluent to the pre-heat well and the count well.
The blood cell analyzer of claim 11, wherein the bubble removing means is a syringe which removes bubbles from the pre-heating well by sucking out a part of the diluent in the pre-heating well and supplying a new diluent to the pre-heating well to refill the pre-heating well.
The hematology analyzer of claim 12, further comprising a first valve, a second valve, and a diluent barrel that provides diluent, wherein:

the injector sucks part of the diluent out of the preheating tank based on the communication between the first valve and the second valve and the communication between the second valve and the preheating tank so as to empty the top of the preheating tank;

the injector discharges the diluent sucked out of the preheating tank through the first pipeline based on the communication between the first valve and the first pipeline;

the injector draws new diluent from the diluent barrel for replenishment of the pre-heat tank with new diluent based on the communication of the first valve with the second valve and the communication of the second valve with the diluent barrel;

the injector replenishes the preheating tank with new diluent drawn out of the diluent bucket based on the communication between the first valve and the second valve and the communication between the second valve and the preheating tank to refill the preheating tank.
The blood cell analyzer of any one of claims 10-13, further comprising:

and the preheating pool temperature adjusting device is used for reducing the temperature of the preheating pool after the counting pool enters the dormancy again.
The blood cell analyzer of any one of claims 10-13, further comprising:

the dormancy time timing device is used for timing the time when the counting pool enters the dormancy again; and

the preheating tank temperature adjusting device is used for adjusting the temperature of the preheating tank based on the length of time for the counting tank to enter the dormancy state so as to reduce the amount of gas separated out from the diluent in the preheating tank during the dormancy state of the counting tank.
The blood cell analyzer according to claim 15, wherein the adjustment of the temperature of the pre-heat well by the pre-heat well temperature adjusting means comprises:

gradually reducing the temperature of the preheating pool as the time for the counting pool to enter the sleep state becomes longer.
The blood cell analyzer according to claim 16, wherein the adjustment of the temperature of the pre-heat well by the pre-heat well temperature adjusting means includes:

setting the temperature of the preheating pool to be a first temperature when the counting pool just enters the dormancy state;

when the time period that the counting pool enters the dormancy reaches a first time period, setting the temperature of the preheating pool to be a second temperature, wherein the second temperature is lower than the first temperature; and

and when the time period that the counting pool enters the dormancy state reaches a second time period, setting the temperature of the preheating pool to be a third temperature, wherein the second time period is greater than the first time period, and the third temperature is lower than the second temperature.
The blood cell analyzer of any one of claims 10-13 and 15-17, further comprising:

the dormancy time timing device is used for timing the time of the counting pool entering dormancy after the counting pool enters dormancy; and is

The bubble discharge device is also used for: after the counting cell enters the sleep mode, the bubbles in the preheating cell are discharged at preset time intervals based on the timing of the sleep time timing device.
The blood cell analyzer of any one of claims 10-13 and 15-17, wherein the bubble evacuation device is further configured to:

and before each time of counting and classifying the white blood cells in the counting pool, discharging the bubbles in the preheating pool.