CN114460326A - Reagent replenishing device and reagent replenishing method for sample analyzer - Google Patents

Reagent replenishing device and reagent replenishing method for sample analyzer Download PDF

Info

Publication number
CN114460326A
CN114460326A CN202210375581.1A CN202210375581A CN114460326A CN 114460326 A CN114460326 A CN 114460326A CN 202210375581 A CN202210375581 A CN 202210375581A CN 114460326 A CN114460326 A CN 114460326A
Authority
CN
China
Prior art keywords
liquid
control valve
negative pressure
reagent
pump
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210375581.1A
Other languages
Chinese (zh)
Inventor
刘希昌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Dymind Biotechnology Co Ltd
Original Assignee
Shenzhen Dymind Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Dymind Biotechnology Co Ltd filed Critical Shenzhen Dymind Biotechnology Co Ltd
Priority to CN202210375581.1A priority Critical patent/CN114460326A/en
Publication of CN114460326A publication Critical patent/CN114460326A/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002Reagent dispensers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009Characterised by arrangements for controlling the aspiration or dispense of liquids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016Control of the volume dispensed or introduced

Abstract

The present application provides a reagent replenishing device of a sample analyzer and a reagent replenishing method thereof, the reagent replenishing device including: the liquid sucking assembly comprises a negative pressure assembly, a dosing pump, a one-way valve and a reagent bottle which are sequentially connected, the negative pressure assembly is used for providing negative pressure for the dosing pump so that the dosing pump sucks quantitative liquid from the reagent bottle, and the one-way valve is used for enabling the liquid in the reagent bottle to flow to the dosing pump and preventing the liquid in the dosing pump from flowing back; the liquid discharge assembly is connected with the fixed displacement pump and is used for discharging liquid in the fixed displacement pump. The reagent replenishing device has the advantages that the structure is simple, the influence of negative pressure fluctuation caused by other negative pressure consumption components on the liquid suction amount of the constant delivery pump can be avoided, the requirement of the constant delivery pump on negative pressure is reduced, and the reliability of a sample analyzer is improved.

Description

Reagent replenishing device and reagent replenishing method for sample analyzer
Technical Field
The present application relates to the field of medical devices, and in particular, to a reagent replenishing device and a reagent replenishing method for a sample analyzer.
Background
In the conventional blood cell analyzer, a quantitative pump is generally used as a reagent supply device to supply a quantitative liquid. The constant delivery pump is connected with the negative pressure to pump liquid, and the constant delivery pump is connected with the positive pressure to push out liquid, thereby realizing the quantitative liquid supply.
However, the reagent replenishing device in the prior art needs to have a certain requirement on the negative pressure of the dosing pump in a period of time immediately before the liquid discharging action, and because other negative pressure consuming device components exist in the sample analyzer, the negative pressure consuming device components may affect the negative pressure of the dosing pump in a period of time immediately before the liquid discharging action, which causes great difficulty in time sequence arrangement.
Disclosure of Invention
The application provides a reagent supplementing device and a reagent supplementing method of a sample analyzer, which can reduce the influence of negative pressure fluctuation caused by other negative pressure consumption components in the sample analyzer on the liquid suction amount of a quantitative pump, reduce the time sequence arrangement difficulty of the sample analyzer and improve the reliability of the sample analyzer.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a reagent replenishing device of a sample analyzer, the reagent replenishing device including: the liquid suction assembly comprises a negative pressure assembly, a dosing pump, a one-way valve and a reagent bottle which are sequentially connected, the negative pressure assembly is used for providing negative pressure for the dosing pump so that the dosing pump sucks quantitative liquid from the reagent bottle, and the one-way valve is used for enabling the liquid in the reagent bottle to flow to the dosing pump and preventing the liquid in the dosing pump from flowing back; and the liquid discharge assembly is connected with the quantitative pump and is used for discharging the liquid in the quantitative pump.
Further, be provided with the diaphragm in the constant delivery pump, the diaphragm divides the constant delivery pump into air chamber and liquid chamber, and wherein the air chamber is connected to the negative pressure subassembly, and the liquid chamber is connected to the check valve.
Further, the reagent supplementing device also comprises a first control valve and a second control valve, wherein the first end of the first control valve is connected with the negative pressure component, the second end of the first control valve is connected with the air chamber, and the first control valve is used for controlling the communication and the closing of the negative pressure component and the air chamber; the first end of the second control valve is connected with the liquid chamber, the second end of the second control valve is connected with the one-way valve, and the second control valve is used for controlling the communication and the closing of the liquid chamber and the reagent bottle.
Further, the liquid discharge assembly comprises a positive pressure assembly and a reaction tank, the first control valve and the second control valve are three-way valves, the positive pressure assembly is connected with the third end of the first control valve, and one end of the reaction tank is connected with the third end of the second control valve.
Further, flowing back subassembly still includes the waste liquid pond, and the first end in waste liquid pond is connected with the other end of reaction tank, and negative pressure subassembly and malleation subassembly are connected with the second end in waste liquid pond respectively.
Further, the sample analyzer still includes the sampling needle, and the sampling needle also is connected with the negative pressure subassembly, and the negative pressure subassembly provides the negative pressure for the sampling needle to make the sampling needle draw the sample from the test tube.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a reagent replenishing method for a sample analyzer, the reagent replenishing method including, based on the reagent replenishing apparatus of any one of the above embodiments: in any time period meeting the preset conditions before the liquid is discharged by the quantitative pump, the quantitative pump sucks quantitative liquid from the reagent bottle through the negative pressure assembly; the liquid in the metering pump is prevented from flowing back through the one-way valve; the liquid in the quantitative pump is discharged through the liquid discharge assembly.
Further, be provided with the diaphragm in the dosing pump, the diaphragm divides the dosing pump into air chamber and liquid chamber, wherein negative pressure subassembly connects the air chamber, the liquid chamber is connected to the check valve, reagent supplementary device still includes first control valve and second control valve, the flowing back subassembly includes malleation subassembly and reaction tank, negative pressure subassembly is connected to the first end of first control valve, the air chamber is connected to the second end of first control valve, malleation subassembly is connected to the third end of first control valve, the liquid chamber is connected to the first end of second control valve, the check valve is connected to the second end of second control valve, the third end connection reaction tank of second control valve, through negative pressure subassembly, make the dosing pump follow reagent bottle and absorb quantitative liquid, include: controlling the first end of the first control valve to be communicated with the second end of the first control valve, and controlling the first end of the second control valve to be communicated with the second end of the second control valve; the quantitative pump sucks quantitative liquid from the reagent bottle through the negative pressure component; discharging the liquid in the dosing pump through a discharge assembly, comprising: controlling the second end of the first control valve to be communicated with the third end of the first control valve, and controlling the first end of the second control valve to be communicated with the third end of the second control valve; and pumping the liquid in the quantitative pump to the reaction tank through the positive pressure component.
Further, after the step of pumping the liquid in the quantitative pump to the reaction tank through the positive pressure assembly, the reagent supplementing method further comprises the following steps: controlling the first end of the first control valve to be communicated with the second end of the first control valve, and controlling the first end of the second control valve to be communicated with the second end of the second control valve; and in a time period which meets the preset condition before the next liquid drainage, the quantitative pump is enabled to suck quantitative liquid from the reagent bottle through the negative pressure assembly.
Further, the liquid discharge assembly further comprises a waste liquid pool, the other end of the reaction pool is connected with the first end of the waste liquid pool, the negative pressure assembly and the positive pressure assembly are respectively connected with the second end of the waste liquid pool, and the reagent supplementing method further comprises the following steps: sucking liquid in the reaction tank into a waste liquid tank through a negative pressure assembly; and discharging the liquid in the waste liquid pool through the positive pressure component.
The beneficial effect of this application is: in contrast to the prior art, the reagent replenishing device of the sample analyzer of the present application includes: the liquid sucking assembly comprises a negative pressure assembly, a dosing pump, a one-way valve and a reagent bottle which are sequentially connected, the negative pressure assembly is used for providing negative pressure for the dosing pump so that the dosing pump sucks quantitative liquid from the reagent bottle, and the one-way valve is used for enabling the liquid in the reagent bottle to flow to the dosing pump and preventing the liquid in the dosing pump from flowing back; the liquid discharge assembly is connected with the fixed displacement pump and is used for discharging liquid in the fixed displacement pump. The sample analyzer has the advantages that the structure is simple, the influence of negative pressure fluctuation caused by other negative pressure consumption components in the sample analyzer on the liquid suction amount of the constant delivery pump can be avoided, the requirement of the constant delivery pump on negative pressure is reduced, the difficulty of time sequence arrangement of the sample analyzer is reduced, and the reliability of the sample analyzer is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a schematic diagram of a prior art timing arrangement of a sample analyzer;
FIG. 2 is a schematic diagram of the structure of one embodiment of a reagent replenishment device for a sample analyzer provided herein;
FIG. 3 is a schematic diagram of a timing arrangement of a reagent replenishment arrangement of a sample analyzer provided herein;
FIG. 4 is a schematic diagram of another embodiment of a reagent replenishment assembly for a sample analyzer provided herein;
FIG. 5 is a schematic flow chart diagram illustrating one embodiment of a method for reagent replenishment of a sample analyzer provided herein;
FIG. 6 is a flowchart illustrating an embodiment of step S11 in FIG. 5;
FIG. 7 is a flowchart illustrating an embodiment of step S13 in FIG. 5.
Detailed Description
The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that if directional indications (such as up, down, left, right, front, and back … …) are referred to in the embodiments of the present application, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in the embodiments of the present application, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present application.
In the conventional sample analyzer, as shown in fig. 1, in the total timing T of the operation of the sample analyzer, the liquid suction time of the fixed displacement pump is T1, the liquid discharge time of the fixed displacement pump is T2, and the liquid suction time T1 is prior to the liquid discharge time T2 and is immediately adjacent to the liquid discharge time T2. In order to ensure the liquid suction accuracy of the fixed displacement pump, a certain range of negative pressure fluctuation needing to be controlled in T1 time before the fixed displacement pump performs liquid discharge needs to be ensured in the time sequence total operation period T, which has requirements on the negative pressure consumed by other negative pressure consuming device components in the time period T1, and thus certain difficulties are caused to the design and time sequence arrangement of the instrument.
The application provides a sample analyzer's reagent supplementing device, this reagent supplementing device can avoid the influence of negative pressure fluctuation brought by other negative pressure consumer parts to the constant delivery pump liquid absorption volume, reduces the requirement of constant delivery pump to the negative pressure, improves sample analyzer's reliability. Among others, sample analyzers may include, but are not limited to: blood cell analyzer, biochemical analyzer, and immunity analyzer. Referring to fig. 2, fig. 2 is a schematic structural diagram of an embodiment of a reagent replenishing device of a sample analyzer. Specifically, the reagent replenishing device 10 includes: a wicking assembly 11 and a drainage assembly 12.
The pipetting assembly 11 comprises a negative pressure assembly 111, a dosing pump 112, a one-way valve 113 and a reagent bottle 114 connected in sequence.
The reagent bottle 114 is used for placing a liquid reagent, and the negative pressure assembly 111 is used for providing negative pressure for the quantitative pump 112 so that the quantitative pump 112 sucks quantitative liquid from the reagent bottle 114. The check valve 113 is used to flow the liquid in the reagent bottle 114 to the dosing pump 112, and the check valve 113 can prevent the liquid in the dosing pump 112 from flowing back. The liquid discharge assembly 12 is connected to the quantitative pump 112, and is used for discharging the liquid sucked by the quantitative pump 112 to the reaction cell 30 of the sample analyzer.
In the above embodiment, as shown in fig. 3, the check valve 113 is provided between the fixed displacement pump 112 and the reagent bottle 114, so that the fixed displacement pump 112 satisfies the negative pressure fluctuation range of the liquid suction of the fixed displacement pump 112 in an arbitrary time period T1 before the liquid is discharged in the total operation timing T of the sample analyzer, thereby ensuring the liquid suction accuracy of the fixed displacement pump 112, reducing the difficulty of the instrument design and the timing arrangement of the sample analyzer, and improving the reliability of the sample analyzer.
Further, as shown in FIG. 2, a diaphragm 1121 is provided in the fixed displacement pump 112, and the diaphragm 1121 divides the fixed displacement pump 112 into an air chamber 1122 and a liquid chamber 1123. That is, the gas chamber 1122 and the liquid chamber 1123 are located on both sides of the diaphragm 1121, respectively. The diaphragm 1121 is movable by a pressure difference between the liquid chamber 1123 and the gas chamber 1122.
Further, as shown in FIG. 2, a negative pressure assembly 111 is coupled to the air chamber 1122, and the negative pressure assembly 111 is configured to vary the pressure of the air chamber 1122. The reagent bottle 114 is connected to the liquid chamber 1123 via the check valve 113, and the liquid in the reagent bottle 114 flows to the liquid chamber 1123 by the negative pressure.
Further, as shown in fig. 2, the reagent replenishing device 10 further includes a first control valve 115 and a second control valve 116, and the draining assembly 12 includes a positive pressure assembly 121 and a reaction cell 30. The first control valve 115 and the second control valve 116 may be three-way valves.
Specifically, a first end of the first control valve 115 is connected to the negative pressure assembly 111, a second end of the first control valve 115 is connected to the air chamber 1122, and a third end of the first control valve 115 is connected to the positive pressure assembly 121. A first end of the second control valve 116 is connected to the liquid chamber 1123, and a second end of the second control valve 116 is connected to the check valve 113. The third end of the second control valve 116 is connected to the reaction cell 30 of the sample analyzer.
Further, as shown in fig. 2, the negative pressure assembly 111 includes a negative pressure source 1111 and a negative pressure regulating valve 1112, wherein the negative pressure source 1111 is connected to one end of the negative pressure regulating valve 1112, and the other end of the negative pressure regulating valve 1112 is connected to the fixed displacement pump 112 through the first control valve 115. The other end of the negative pressure regulating valve 1112 may also be connected to other negative pressure consuming components 20 in the sample analyzer and provide negative pressure to the other negative pressure consuming components 20. The other negative pressure consuming part 20 may be another fixed displacement pump, or the other negative pressure consuming part 20 may further include a sampling needle, that is, the negative pressure component 111 is also connected to the sampling needle (not shown), and provides negative pressure to the sampling needle, so that the sampling needle can suck a sample from the test tube.
For example, when the reagent replenishing device 10 is operated, the negative pressure module 111 is adjusted to a desired target pressure, the first end of the first control valve 115 is communicated with the second end of the first control valve 115, and then the pressure is supplied to the air chamber 1122 of the metering pump 112 and other negative pressure consuming components 20 in the sample analyzer. The check valve 113 is used to prevent the liquid in the fixed displacement pump 112 from flowing back, and as shown in fig. 3, in the total period T allowed by the timing of the sample analyzer, the liquid suction accuracy of the fixed displacement pump 112 can be ensured as long as any time period T1 satisfies the negative pressure fluctuation range of the liquid suction of the fixed displacement pump 112 before the time period T2 of the liquid discharge.
When liquid is discharged within the time period t2, the second end of the first control valve 115 is communicated with the third end of the first control valve 115, the first end of the second control valve 116 is communicated with the third end of the second control valve 116, the positive pressure component 121 inputs positive pressure into the gas chamber 1122 of the fixed displacement pump 112, and the liquid in the fixed displacement pump 112 is discharged to the reaction cell 30 of the sample analyzer through the second control valve 116. After the metering pump 112 finishes discharging liquid, the first control valve 115 and the second control valve 116 are reset, that is, the first end of the first control valve 115 is communicated with the second end of the first control valve 115, the first end of the second control valve 116 is communicated with the second end of the second control valve 116, before next liquid discharging, the reagent in the reagent bottle 114 flows to the metering pump 112 through the one-way valve 113 under the action of the negative pressure assembly 111, and when the negative pressure fluctuation range of the liquid suction of the metering pump 112 is met within a certain time period t1, the liquid suction precision of the metering pump 112 is ensured. Then the next drainage is carried out, and the process is repeated.
In the above embodiment, the reagent replenishing device 10 of the sample analyzer has a simple structure, and can avoid the influence of negative pressure fluctuation caused by other negative pressure consuming components 20 on the liquid suction amount of the fixed displacement pump 112, reduce the requirement of the fixed displacement pump 112 on negative pressure, reduce the difficulty of time sequence arrangement of the sample analyzer, and improve the reliability of the sample analyzer.
Further, in another embodiment, as shown in fig. 4, the reaction cell 30 includes a first reaction cell 31 and a second reaction cell 32, and a third end of the second control valve 116 is connected to the first reaction cell 31 and the second reaction cell 32 through a third control valve 34, so that the dosing pump 112 provides a fixed amount of liquid, such as a reagent, in the first reaction cell 31 and the second reaction cell 32, respectively. Specifically, the third end of the second control valve 116 is connected to the first end of the third control valve 34, the second end of the third control valve 34 is connected to one end of the first reaction tank 31, and the third end of the third control valve 34 is connected to one end of the second reaction tank 32. When the first end and the second end of the third control valve 34 are communicated, the fixed displacement pump 112 supplies a fixed amount of liquid into the first reaction tank 31; when the first end of the third control valve 34 is communicated with the third end, the fixed displacement pump 112 supplies a fixed amount of liquid into the second reaction cell 32.
In other embodiments, the reaction chamber 30 may further include three or more sub-reaction chambers, and the fixed displacement pump 112 may input a fixed amount of liquid into different sub-reaction chambers through control valves. This is not a list.
Further, as shown in fig. 4, the drainage assembly 12 may further include a waste liquid tank 33, and the liquid in the reaction tank 30 is drained into the waste liquid tank 33 after the test is completed.
Further, the first reaction cell 31 may be connected to a first end of the waste liquid tank 33 through a fourth control valve 35, and the second reaction cell 32 may be connected to a first end of the waste liquid tank 33 through a fifth control valve 36. The negative pressure assembly 111 and the positive pressure assembly 121 are connected to the second end of the waste liquid tank 33 through a sixth control valve 37.
Specifically, the negative pressure assembly 111 is connected to a first end of the sixth control valve 37, the positive pressure assembly 121 is connected to a second end of the sixth control valve 37, and a third end of the sixth control valve 37 is connected to a second end of the waste liquid tank 33.
When the reaction cell 30 discharges liquid, the first end of the sixth control valve 37 communicates with the third end, that is, the negative pressure module 111 communicates with the waste liquid pool 33, the liquid in the reaction cell 30 is sucked into the waste liquid pool 33 by the action of the negative pressure module 111, and when the waste liquid in the waste liquid pool 33 is discharged, the second end of the sixth control valve 37 communicates with the third end, that is, the positive pressure module 121 communicates with the waste liquid pool 33, and the waste liquid in the waste liquid pool 33 is discharged by the positive pressure module 121. Further, the third end of the waste liquid tank 33 may be connected to an external waste liquid receiving device (not shown) through a seventh control valve 38.
In this embodiment, the waste liquid tank 33 completes the liquid discharging operation of the reaction tank 30 and the waste liquid tank 33 through the positive pressure component 121 and the negative pressure component 111, and has a simple structure and a low cost.
The present application further provides a reagent replenishing method for a sample analyzer, which is based on the reagent replenishing device of any of the above embodiments, and please refer to the description of any of the above embodiments for the structure of the reagent replenishing device, which is not described herein again. Specifically, as shown in fig. 5, the reagent replenishing method includes:
s11: in the time period that satisfies the preset condition wantonly before the quantitative pump flowing back, through negative pressure subassembly, make quantitative pump absorb quantitative liquid from the reagent bottle.
As shown in figure 3, before the time t2 when the dosing pump discharges liquid, in any time period t1 when a preset condition is met, the preset condition can be that a negative pressure fluctuation range of liquid suction of the dosing pump is met, a fixed amount of liquid can be sucked in the dosing pump through the negative pressure assembly, and the liquid sucked into the dosing pump cannot flow back under the action of the one-way valve, so that the precision of the dosing pump is ensured.
In a specific embodiment, a diaphragm is arranged in the quantitative pump, the diaphragm divides the quantitative pump into an air chamber and a liquid chamber, wherein the negative pressure component is connected with the air chamber, the one-way valve is connected with the liquid chamber, the reagent supplementing device further comprises a first control valve, a second control valve and a reaction pool, the first end of the first control valve is connected with the negative pressure component, the second end of the first control valve is connected with the air chamber, the first end of the second control valve is connected with the liquid chamber, and the second end of the second control valve is connected with the one-way valve. As shown in fig. 6, the step of causing the quantitative pump to suck a quantitative liquid from the reagent bottle by the negative pressure assembly includes:
s111: the first end of the first control valve is controlled to be communicated with the second end of the first control valve, and the first end of the second control valve is controlled to be communicated with the second end of the second control valve.
S112: the quantitative pump sucks quantitative liquid from the reagent bottle through the negative pressure component.
When the first end and the second end of the first control valve are communicated, the negative pressure assembly is communicated with the air chamber, when the first end and the second end of the second control valve are communicated, the reagent bottle is communicated with the liquid chamber, and the quantitative liquid can be sucked from the reagent bottle by the quantitative pump under the action of the negative pressure assembly.
In the implementation, the first control valve and the second control valve are arranged, so that the liquid suction process is controlled conveniently.
S12: the liquid in the dosing pump is prevented from flowing back by the one-way valve.
The liquid reflux in the constant delivery pump can be prevented under the action of the one-way valve after the constant delivery pump sucks liquid under the action of the negative pressure assembly, so that the liquid suction precision of the constant delivery pump is ensured. The influence of negative pressure fluctuation brought by other negative pressure consumption components on the liquid suction capacity of the constant delivery pump is avoided, and the requirement of the constant delivery pump on the negative pressure is reduced.
S13: the liquid in the quantitative pump is discharged through the liquid discharge assembly.
When the liquid is discharged by the fixed displacement pump, the liquid in the fixed displacement pump can be discharged through the liquid discharge assembly. Specifically, flowing back subassembly includes the malleation subassembly, and first control valve and second control valve are the three-way valve, and the third end of first control valve is connected to the malleation subassembly, and the third end of second control valve is connected the reaction tank.
In one specific embodiment, as shown in fig. 7, the step of discharging the liquid in the fixed displacement pump through the liquid discharge assembly comprises:
s131: and controlling the second end of the first control valve to be communicated with the third end of the first control valve, and controlling the first end of the second control valve to be communicated with the third end of the second control valve.
S132: and pumping the liquid in the quantitative pump to the reaction tank through the positive pressure component.
S133: and sucking the liquid in the reaction tank into the waste liquid tank through the negative pressure assembly.
S134: and discharging the liquid in the waste liquid pool through the positive pressure component.
The liquid discharge assembly is simple in liquid discharge process. The reagent supplementing device firstly controls the second end of the first control valve to be communicated with the third end of the first control valve so as to enable the positive pressure component to be communicated with the dosing pump, and controls the first end of the second control valve to be communicated with the third end of the second control valve so as to enable the dosing pump to be communicated with the reaction pool. The liquid in the quantitative pump is quantitatively pumped into the reaction tank through the positive pressure component. When the reaction tank was when flowing back, the first end and the third end intercommunication of sixth control valve, promptly, made negative pressure components and waste liquid pond intercommunication, liquid in making the reaction tank through negative pressure components's effect was inhaled in the waste liquid pond, when discharging the waste liquid in the waste liquid pond, the second end and the third end intercommunication of sixth control valve, promptly, make malleation subassembly and waste liquid pond intercommunication, through the waste liquid discharge of malleation subassembly in with the waste liquid pond.
Further, after the liquid in the quantitative pump is pumped out, the reagent supplementing device can control the first end of the first control valve to be communicated with the second end of the first control valve, and control the first end of the second control valve to be communicated with the second end of the second control valve, so that the quantitative pump can suck a quantitative liquid from the reagent bottle again through the negative pressure component before the next liquid drainage.
The reagent supplementing method in the embodiment is simple in process, the supplementing process of the reagent is not influenced by negative pressure fluctuation caused by other negative pressure consumption components on the liquid suction amount of the quantitative pump, the requirement of the quantitative pump on the negative pressure is reduced, the difficulty of time sequence arrangement of the sample analyzer is reduced, and the reliability of the sample analyzer is improved.
The above embodiments are merely examples and are not intended to limit the scope of the present disclosure, and all modifications, equivalents, and flow charts using the contents of the specification and drawings of the present disclosure, which are directly or indirectly applied to other related technical fields, are included in the scope of the present disclosure.

Claims (10)

1. A reagent replenishing device for a sample analyzer, comprising:
the liquid sucking assembly comprises a negative pressure assembly, a dosing pump, a one-way valve and a reagent bottle which are sequentially connected, the negative pressure assembly is used for providing negative pressure for the dosing pump so that the dosing pump sucks quantitative liquid from the reagent bottle, and the one-way valve is used for enabling the liquid in the reagent bottle to flow to the dosing pump and preventing the liquid in the dosing pump from flowing back;
and the liquid discharge assembly is connected with the quantitative pump and is used for discharging the liquid in the quantitative pump.
2. The reagent replenishing device of claim 1, wherein a diaphragm is disposed within the dosing pump, the diaphragm dividing the dosing pump into an air chamber and a liquid chamber, wherein the negative pressure assembly is connected to the air chamber and the one-way valve is connected to the liquid chamber.
3. The reagent replenishing device of claim 2, further comprising a first control valve and a second control valve,
the first end of the first control valve is connected with the negative pressure component, the second end of the first control valve is connected with the air chamber, and the first control valve is used for controlling the communication and the closing of the negative pressure component and the air chamber;
the first end of the second control valve is connected with the liquid chamber, the second end of the second control valve is connected with the one-way valve, and the second control valve is used for controlling the communication and the closing of the liquid chamber and the reagent bottle.
4. The reagent replenishing device of claim 3, wherein the liquid discharge assembly comprises a positive pressure assembly and a reaction cell, the first control valve and the second control valve are three-way valves, the positive pressure assembly is connected with the third end of the first control valve, and one end of the reaction cell is connected with the third end of the second control valve.
5. The reagent replenishing device of claim 4, wherein the liquid draining assembly further comprises a waste liquid tank, a first end of the waste liquid tank is connected with the other end of the reaction tank, and the negative pressure assembly and the positive pressure assembly are respectively connected with a second end of the waste liquid tank.
6. The reagent replenishment device of claim 1, wherein the sample analyzer further comprises a sampling needle, the sampling needle also being coupled to the negative pressure assembly, the negative pressure assembly providing negative pressure to the sampling needle to cause the sampling needle to draw a sample from a test tube.
7. A reagent replenishing method for a sample analyzer, based on the reagent replenishing device according to any one of claims 1 to 6, comprising:
in any time period meeting preset conditions before the quantitative pump discharges liquid, enabling the quantitative pump to suck quantitative liquid from the reagent bottle through the negative pressure assembly;
preventing backflow of the liquid in the dosing pump by a one-way valve;
discharging the liquid in the dosing pump through a liquid discharge assembly.
8. The reagent replenishing method of claim 7, wherein a diaphragm is provided in the metering pump, the diaphragm dividing the metering pump into a gas chamber and a liquid chamber, wherein the negative pressure component is connected to the gas chamber, the check valve is connected to the liquid chamber, the reagent replenishing apparatus further comprises a first control valve and a second control valve, the liquid discharge component comprises a positive pressure component and a reaction cell, a first end of the first control valve is connected to the negative pressure component, a second end of the first control valve is connected to the gas chamber, a third end of the first control valve is connected to the positive pressure component, a first end of the second control valve is connected to the liquid chamber, a second end of the second control valve is connected to the check valve, and a third end of the second control valve is connected to the reaction cell,
the negative pressure component enables the quantitative pump to suck quantitative liquid from the reagent bottle, and the negative pressure component comprises: controlling a first end of the first control valve to be communicated with a second end of the first control valve, and controlling a first end of the second control valve to be communicated with a second end of the second control valve; causing the dosing pump to draw a metered amount of the liquid from the reagent bottle through the negative pressure assembly;
the discharging the liquid in the dosing pump by the liquid discharge assembly comprises: controlling the second end of the first control valve to be communicated with the third end of the first control valve, and controlling the first end of the second control valve to be communicated with the third end of the second control valve;
the liquid in the quantitative pump is pumped to the reaction tank through the positive pressure component.
9. The method of claim 8, wherein after the step of pumping the liquid in the dosing pump to the reaction cell by the positive pressure assembly, the method further comprises:
controlling the first end of the first control valve to be communicated with the second end of the first control valve, and controlling the first end of the second control valve to be communicated with the second end of the second control valve;
and in any time period meeting the preset condition before next liquid drainage, the quantitative pump sucks quantitative liquid from the reagent bottle through the negative pressure assembly.
10. The reagent replenishing method of claim 8, wherein the liquid discharge assembly further comprises a waste liquid tank, the other end of the reaction tank is connected to a first end of the waste liquid tank, and the negative pressure assembly and the positive pressure assembly are respectively connected to a second end of the waste liquid tank, and the reagent replenishing method further comprises:
sucking the liquid in the reaction tank into the waste liquid tank through the negative pressure assembly;
discharging the liquid in the waste liquid pool through the positive pressure component.
CN202210375581.1A 2022-04-11 2022-04-11 Reagent replenishing device and reagent replenishing method for sample analyzer Pending CN114460326A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210375581.1A CN114460326A (en) 2022-04-11 2022-04-11 Reagent replenishing device and reagent replenishing method for sample analyzer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210375581.1A CN114460326A (en) 2022-04-11 2022-04-11 Reagent replenishing device and reagent replenishing method for sample analyzer

Publications (1)

Publication Number Publication Date
CN114460326A true CN114460326A (en) 2022-05-10

Family

ID=81417252

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210375581.1A Pending CN114460326A (en) 2022-04-11 2022-04-11 Reagent replenishing device and reagent replenishing method for sample analyzer

Country Status (1)

Country Link
CN (1) CN114460326A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110031644A (en) * 2017-06-30 2019-07-19 深圳迈瑞生物医疗电子股份有限公司 Agent delivery device, sample analyser and agent delivery method
CN111830269A (en) * 2019-04-17 2020-10-27 深圳迈瑞生物医疗电子股份有限公司 Reagent supply device, sample analyzer and reagent supply method thereof
CN112782419A (en) * 2020-12-09 2021-05-11 大连科欣仪表研究所 Metering and dosing method for reagent of analyzer
CN112857949A (en) * 2019-11-12 2021-05-28 深圳迈瑞生物医疗电子股份有限公司 Liquid discharging method of quantitative device, dye liquid preparation method and computer storage medium
CN214374851U (en) * 2020-12-23 2021-10-08 重庆中元汇吉生物技术有限公司 Liquid supply device and sample analyzer
CN113759139A (en) * 2020-06-01 2021-12-07 深圳迈瑞生物医疗电子股份有限公司 Sample analyzer and liquid supply method thereof
CN215711746U (en) * 2021-07-30 2022-02-01 深圳市帝迈生物技术有限公司 Reagent filling device
CN114112805A (en) * 2020-09-01 2022-03-01 深圳迈瑞生物医疗电子股份有限公司 Blood cell analyzer

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110031644A (en) * 2017-06-30 2019-07-19 深圳迈瑞生物医疗电子股份有限公司 Agent delivery device, sample analyser and agent delivery method
CN111830269A (en) * 2019-04-17 2020-10-27 深圳迈瑞生物医疗电子股份有限公司 Reagent supply device, sample analyzer and reagent supply method thereof
CN112857949A (en) * 2019-11-12 2021-05-28 深圳迈瑞生物医疗电子股份有限公司 Liquid discharging method of quantitative device, dye liquid preparation method and computer storage medium
CN113759139A (en) * 2020-06-01 2021-12-07 深圳迈瑞生物医疗电子股份有限公司 Sample analyzer and liquid supply method thereof
CN114112805A (en) * 2020-09-01 2022-03-01 深圳迈瑞生物医疗电子股份有限公司 Blood cell analyzer
CN112782419A (en) * 2020-12-09 2021-05-11 大连科欣仪表研究所 Metering and dosing method for reagent of analyzer
CN214374851U (en) * 2020-12-23 2021-10-08 重庆中元汇吉生物技术有限公司 Liquid supply device and sample analyzer
CN215711746U (en) * 2021-07-30 2022-02-01 深圳市帝迈生物技术有限公司 Reagent filling device

Similar Documents

Publication Publication Date Title
JP6647264B2 (en) Cartridge for supplying fluid
US20120195799A1 (en) Process analyzer
DE19737173B4 (en) micro-dosing system
CA2392943A1 (en) Chemistry system for a clinical analyzer
US20140139837A1 (en) Spr apparatus with a high performance fluid delivery system
TR200704992T2 (en) Precision dosing device for liquids
CN110038655A (en) A kind of microfluidic control chip and system
CN114460326A (en) Reagent replenishing device and reagent replenishing method for sample analyzer
CN111830269A (en) Reagent supply device, sample analyzer and reagent supply method thereof
CN209673825U (en) Liquid-adding device, analyzer
CN215711746U (en) Reagent filling device
CN109212246B (en) Liquid supply device, sample analyzer, pressure building device and liquid supply method
CN111721956A (en) Liquid path system of full-automatic biochemical analyzer
CN111587371A (en) Sheath flow impedance method particle analyzer and measuring method thereof
WO2020135618A1 (en) Liquid addition apparatus, analyzer, and liquid addition method
CN212780383U (en) Blood cell analyzer
EP0510305A2 (en) Method and apparatus for diluting and mixing liquid specimen
CN107064529A (en) A kind of automatic analysing apparatus and automatic analysis method
CN111617812B (en) Microfluidic substrate, fluid driving method thereof and microfluidic device
CN114112805A (en) Blood cell analyzer
CN110873662A (en) Liquid path system, blending method and sample analysis device
CN216174757U (en) Orifice plate cleaning system
CN109991431A (en) Sample analyser and its agent delivery method
CN213068887U (en) Sample analyzer, liquid adding device and reagent replacing device
CN212459560U (en) Accurate quantitative sample injector suitable for liquid chromatograph

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination