CN114166915B - Horizontal agarose gel electrophoresis sample application color plate - Google Patents

Horizontal agarose gel electrophoresis sample application color plate Download PDF

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Publication number
CN114166915B
CN114166915B CN202111482852.5A CN202111482852A CN114166915B CN 114166915 B CN114166915 B CN 114166915B CN 202111482852 A CN202111482852 A CN 202111482852A CN 114166915 B CN114166915 B CN 114166915B
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China
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electrophoresis
plate
sample application
application color
grooves
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CN202111482852.5A
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CN114166915A (en
Inventor
曹雪
刘守振
曹振
刘一华
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Linyi University
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Linyi University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories

Abstract

The invention discloses a horizontal agarose gel electrophoresis sample application color development plate, which relates to the technical field of biology and comprises an electrophoresis box, wherein an abutting plate is arranged on the top side of the electrophoresis box, and a mounting sleeve I is fixedly arranged at the front end of the left side and the rear end of the right side of the abutting plate. Through the sample application color development board that sets up, sample application color development board usable self colour, and then can clearly present sample application hole position, with this efficiency that improves the sample application, simultaneously through the bottom side with sample application color development board setting at the electrophoresis tank offset plate, can detain the position bottom that puts at the electrophoresis tank offset plate perfectly, and then reach dexterously convenient, do not account for the effect in space, through the joint in silica gel butt joint and joint groove, can realize together butt joint board and apron are fixed, and the later stage can be very convenient dismantle the apron from the butt joint board come, and then when the gel that will use in the later stage is got rid of, can be very convenient take off the apron from the electrophoresis tank.

Description

Horizontal agarose gel electrophoresis sample application color plate
Technical Field
The invention relates to an agarose gel electrophoresis sample application color developing plate, in particular to a horizontal agarose gel electrophoresis sample application color developing plate.
Background
Agarose gel electrophoresis is an electrophoresis method using agar or agarose as a support medium. By means of the molecular sieve effect of agarose gel, nucleic acid fragments are separated due to different molecular weights or molecular shapes and different electrophoretic movement speeds, so that nucleic acid molecules with different molecular weights and different structures can be separated. It is widely used in molecular biology experiments such as DNA, RNA, plasmid and the like identification, polymerase Chain Reaction (PCR), cloning technology and the like. The horizontal agarose gel electrophoresis is one of agarose gel electrophoresis modes, has the advantages of convenient glue preparation and sample addition, simple manufacture, low price and the like, and is widely applied.
At present, a common operation method is that agarose gel is prepared by a gel preparation plate, then a comb for preparing gel is taken down, the gel and the gel preparation plate are put into an electrophoresis tank together, and electrophoresis buffer in the electrophoresis tank needs to be soaked by the agarose gel, and then a pipetting gun is used for spotting in a spotting hole on the gel.
The current agarose gel electrophoresis sample application has some defects and shortcomings when being used in experimental operation, and the specific parts needing improvement are as follows:
1. when the horizontal agarose gel electrophoresis sample is applied, the sample application holes are not easily found when the agarose gel is placed in a buffer solution for sample application due to high transparency of the agarose gel, so that great trouble is caused to operators, especially beginners;
2. the cover plate is inconvenient to separate from the electrophoresis tank, so that used gel is removed in the later stage, and the cover plate is inconvenient to operate when the cover plate is inconvenient to reuse with the electrophoresis tank.
Disclosure of Invention
The invention aims to provide a horizontal agarose gel electrophoresis sample application color developing plate which can be directly buckled at the bottom of a position where an electrophoresis tank gel plate is arranged, and is made of silica gel, and the color can be purple, blue and other colors, so that the phenomenon that a sample hole is not checked during sample application can be effectively avoided, and the sample application efficiency is improved.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a horizontal agarose gel electrophoresis sample application color development board, includes the electrophoresis case, the topside of electrophoresis case is provided with the splice plate, the left side front end and the right side rear end of splice plate are all fixed to be provided with installation cover one, the upside of splice plate is provided with the apron, the left side front end and the right side rear end of apron are all fixed to be provided with installation cover two, be provided with electric pulse splice in the installation cover two, the lateral wall all is provided with electric pulse piece about the electrophoresis case, and two electric pulse pieces are connected with the butt joint piece in two installation covers one respectively, electrophoresis bottom of the case lateral wall middle part is provided with the concave surface, the bottom side inner wall of electrophoresis case is provided with sample application color development board with the position that the concave surface corresponds, concave surface groove has all been seted up to the upside of sample application color development board, the top of sample application color development board is provided with the electrophoresis groove offset comb, square groove has been seted up to the upper surface of apron, a plurality of sample application adapter holes have been seted up to square groove bottom the lateral wall, square groove is furnished with square groove.
As a preferable technical scheme of the invention, a plurality of clamping grooves are formed in the position, close to the edge, of the upper surface of the butt joint plate, and silica gel butt joints are fixedly arranged at the positions, corresponding to the clamping grooves, of the bottom side of the cover plate.
As a preferable technical scheme of the invention, the plurality of clamping grooves are clamped with the corresponding silica gel butt joint, the bottom side of the clamping grooves is provided with the hemispherical groove, the bottom end of the silica gel butt joint is provided with the hemispherical block, and the hemispherical groove is clamped with the hemispherical block.
As a preferable embodiment of the present invention, the spotted color development plate may be set to purple, blue, or the like.
As a preferable technical scheme of the invention, the bottom side of the sample application color development plate is provided with a plurality of limit grooves, each limit groove is internally butted with a limit block, and the limit blocks are fixedly arranged at the positions corresponding to the concave surfaces on the inner wall of the bottom side of the electrophoresis tank.
As a preferable technical scheme of the invention, vertical grooves are formed in the front side wall and the rear side wall of the electrophoresis tank rubber plate, vertical strips are arranged in the two vertical grooves in a sliding manner, and the two vertical strips are respectively and fixedly arranged on the front side wall and the rear side wall of the electrophoresis tank.
As a preferable technical scheme of the invention, the electrophoresis tank, the butt plate, the cover plate, the electrophoresis tank gel plate and the square plate are all made of transparent plastic materials.
As a preferable technical scheme of the invention, a sealing plug is fixedly arranged on the side wall of the square plate, and a handle is fixedly arranged on the upper surface of the square plate.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can utilize the self color of the sample application color plate through the arranged sample application color plate, and further can clearly show the position of the sample application hole, thereby improving the sample application efficiency.
2. According to the invention, the butt-joint plate and the cover plate can be fixed together through the plurality of clamping grooves formed in the butt-joint plate and the plurality of silica gel butt-joint joints fixedly arranged on the bottom side of the cover plate and the clamping grooves, and the cover plate can be conveniently detached from the butt-joint plate in the later stage, so that the cover plate can be conveniently taken down from the electrophoresis box when used gel is removed in the later stage, and the operation is more convenient.
Drawings
FIG. 1 is a schematic view of a three-dimensional separation structure of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic view showing a three-dimensional cross-sectional structure of an electrophoresis tank according to the present invention;
FIG. 4 is a schematic perspective view of an electrophoresis tank gel plate according to the present invention;
FIG. 5 is a schematic perspective view of a color display template according to the present invention.
In the figure: the electrophoresis box 1, the butt joint plate 2, the mounting sleeve I3, the cover plate 4, the mounting sleeve II 5, the clamping groove 6, the silica gel butt joint head 7, the electric pulse butt joint head 8, the electric pulse sheet 9, the concave surface 10, the sample application color development plate 11, the limiting block 12, the limiting groove 13, the concave surface groove 14, the electrophoresis groove rubber plate 15, the comb 16, the vertical groove 17, the vertical bar 18, the square groove 19, the sample application hole 20, the square plate 21 and the sealing plug 22.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, the invention provides a horizontal agarose gel electrophoresis sample application color developing plate, comprising an electrophoresis box 1, wherein a butt plate 2 is arranged on the top side of the electrophoresis box 1, a first mounting sleeve 3 is fixedly arranged at the front end and the rear end of the left side of the butt plate 2, a cover plate 4 is arranged on the upper side of the butt plate 2, a second mounting sleeve 5 is fixedly arranged at the front end and the rear end of the left side of the cover plate 4, an electric pulse butt joint 8 is arranged in the second mounting sleeve 5, the first mounting sleeve 3 and the second mounting sleeve 5 can be used for butt joint of the electric pulse butt joint 8, electric pulse sheets 9 are respectively arranged on the left side wall and the right side wall of the electrophoresis box 1 and are respectively connected with butt sheets in the first mounting sleeve 3, a concave surface 10 is arranged in the middle of the bottom side wall of the electrophoresis box 1, a sample application color developing plate 11 is arranged at the position corresponding to the concave surface 10 on the bottom side inner wall of the electrophoresis box 1, concave grooves 14 are formed on the front side and the rear side of the upper surface of the sample application color plate 11, an electrophoresis tank gel plate 15 is arranged above the sample application color plate 11, a comb 16 is matched with the electrophoresis tank gel plate 15, a square groove 19 is formed on the upper surface of the cover plate 4, a plurality of sample application holes 20 are formed on the bottom side wall of the square groove 19, a square groove 19 is matched with a square plate 21, the sample application color plate 11 is placed on a concave surface 10 of the inner wall of the electrophoresis tank 1, a plurality of limiting blocks 12 are abutted together with a plurality of limiting grooves 13, then electrophoresis buffer is added into the electrophoresis tank 1, agarose gel is prepared through the electrophoresis tank gel plate 15, then the comb 16 for preparing gel is taken down, the agarose gel and the electrophoresis tank gel plate 15 are placed in the middle of the electrophoresis tank 1, meanwhile, a vertical groove 17 and a vertical bar 18 are clamped together, the electrophoresis tank gel plate 15 can be prevented from moving in the electrophoresis tank 1, then the cover plate 4 is covered on the abutting plate 2, simultaneously, a plurality of silica gel butt joints 7 are clamped into a plurality of clamping grooves 6, the cover plate 4 is firmly fixed on the electrophoresis box 1, after the electric pulse butt joints 8 are connected, the square plate 21 can be taken out from the square groove 19 at the moment and then sample application is carried out through sample application holes 20, a plurality of clamping grooves 6 are formed in the upper surface of the butt joint plate 2 near the edge, the silica gel butt joints 7 are fixedly arranged at the positions of the bottom side of the cover plate 4 corresponding to the plurality of clamping grooves 6, the plurality of clamping grooves 6 are clamped with the corresponding silica gel butt joints 7, the bottom side of the clamping grooves 6 is provided with hemispherical grooves, the bottom end of the silica gel butt joint 7 is provided with hemispherical blocks, the hemispherical grooves are clamped with the hemispherical blocks, the silica gel butt joints 7 can be prevented from being easily separated from the clamping grooves 6 through the hemispherical grooves and the hemispherical blocks, the sample application color development plate 11 can be set to be purple, blue and the like, a plurality of limiting grooves 13 are formed in the bottom side of the sample application color plate 11, limiting blocks 12 are butted in each limiting groove 13, the limiting blocks 12 are fixedly arranged at positions corresponding to the concave surfaces 10 on the inner wall of the bottom side of the electrophoresis tank 1, the sample application color plate 11 can be prevented from moving in the electrophoresis tank 1 through the clamping connection of the limiting grooves 13 and the limiting blocks 12, vertical grooves 17 are formed in the front side wall and the rear side wall of the electrophoresis tank rubber plate 15, vertical strips 18 are slidably arranged in the two vertical grooves 17, the two vertical strips 18 are fixedly arranged on the inner wall of the front side and the rear side of the electrophoresis tank 1 respectively, the electrophoresis tank rubber plate 15 can be prevented from moving in the electrophoresis tank 1 through the clamping connection of the vertical strips 18 and the vertical grooves 17, the electrophoresis tank 1, the butt joint plate 2, the cover plate 4, the electrophoresis tank rubber plate 15 and the square plate 21 are made of transparent plastic materials, the transparent plastic materials are convenient to observe during glue application, the sealing plug 22 is fixedly arranged on the side wall of the square plate 21, the handle is fixedly arranged on the upper surface of the square plate 21, the square plate 21 can be firmly fixed in the square groove 19 through the arranged sealing plug 22, and the square plate 21 can be conveniently taken out from the square groove 19 through the arranged handle.
When the electrophoresis box is specifically used, the sample application color development plate 11 is placed on the concave surface 10 on the inner wall of the electrophoresis box 1, the limiting blocks 12 are abutted with the limiting grooves 13, then electrophoresis buffer is added into the electrophoresis box 1, agarose gel is prepared through the electrophoresis groove gel plate 15, then the comb 16 for glue preparation is taken down, the agarose gel and the electrophoresis groove gel plate 15 are placed in the middle of the electrophoresis box 1, the vertical grooves 17 and the vertical strips 18 are clamped together, the electrophoresis groove gel plate 15 can be prevented from moving in the electrophoresis box 1, then the cover plate 4 is covered on the abutting plate 2, a plurality of silica gel abutting joints 7 are clamped into the clamping grooves 6, the cover plate 4 is firmly fixed on the electrophoresis box 1, after the electrophoresis abutting joint 8 is connected, the square plate 21 can be taken out from the square groove 19, and then sample application operation is carried out through the sample application holes 20.
In the description of the present invention, it should be understood that the orientation or positional relationship indicated is based on the orientation or positional relationship shown in the drawings, and is merely for convenience in describing the present invention and simplifying the description, and does not indicate or imply that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the present invention, unless explicitly specified and defined otherwise, for example, it may be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intermediaries, or in communication with each other or in interaction with each other, unless explicitly defined otherwise, the meaning of the terms described above in this application will be understood by those of ordinary skill in the art in view of the specific circumstances.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (1)

1. The utility model provides a horizontal agarose gel electrophoresis sample application color development board, includes electrophoresis case (1), its characterized in that: the electrophoresis box comprises an electrophoresis box body, and is characterized in that an abutting plate (2) is arranged on the top side of the electrophoresis box body (1), an installation sleeve I (3) is fixedly arranged at the front end of the left side and the rear end of the right side of the abutting plate (2), a cover plate (4) is arranged on the upper side of the abutting plate (2), an installation sleeve II (5) is fixedly arranged at the front end of the left side and the rear end of the cover plate (4), an electric pulse abutting joint (8) is arranged in the installation sleeve II (5), electric pulse sheets (9) are arranged on the left side wall and the right side wall of the electrophoresis box body (1), the two electric pulse sheets (9) are respectively connected with the abutting plates in the two installation sleeves I (3), a concave surface (10) is arranged in the middle of the bottom side wall of the electrophoresis box body (1), a sample application color display plate (11) is arranged at the position corresponding to the concave surface (10), concave surface grooves (14) are formed in the front and rear sides of the upper surface of the sample application color display plate (11), electric pulse sheets (9) are respectively arranged above the sample application color display plate (11), a comb-shaped groove (19) is formed in the middle of the bottom side wall (1), and a comb-shaped groove (19) is formed in the middle of the electrophoresis box body (1), and the comb-shaped groove (19) is provided with a plurality of square grooves (19).
A plurality of clamping grooves (6) are formed in the upper surface of the butt joint plate (2) close to the edge, silica gel butt joints (7) are fixedly arranged at the positions, corresponding to the clamping grooves (6), of the bottom side of the cover plate (4), the clamping grooves (6) are clamped with the corresponding silica gel butt joints (7), hemispherical grooves are formed in the bottom side of the clamping grooves (6), hemispherical blocks are arranged at the bottom ends of the silica gel butt joints (7), and the hemispherical grooves are clamped with the hemispherical blocks;
the sample application color development board (11) is set to purple or blue, a plurality of spacing grooves (13) have been seted up to the downside of sample application color development board (11), every all dock stopper (12) in spacing groove (13), and on the bottom side inner wall of a plurality of stopper (12) all fixed settings in electrophoresis case (1) and concave surface (10) corresponding position, vertical groove (17) have all been seted up to the front and back lateral wall of electrophoresis tank offset plate (15), two all slide in vertical groove (17) and be provided with vertical bar (18), and two vertical bars (18) are fixed respectively to be set up at the front and back side inner wall of electrophoresis case (1), butt plate (2), apron (4), electrophoresis tank offset plate (15) and square board (21) are transparent plastics material, fixedly on the lateral wall of square board (21) be provided with sealing plug (22), and the upper surface fixing of square board (21) is provided with the handle.
CN202111482852.5A 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate Active CN114166915B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111482852.5A CN114166915B (en) 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111482852.5A CN114166915B (en) 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate

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CN114166915A CN114166915A (en) 2022-03-11
CN114166915B true CN114166915B (en) 2023-12-01

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
CN201222053Y (en) * 2008-07-16 2009-04-15 孙朝阳 Disposable electrophoresis rubber-making plate
CN104807873A (en) * 2015-04-17 2015-07-29 贵州大学 Multifunctional agarose gel electrophoresis tank
CN205139079U (en) * 2015-10-30 2016-04-06 满朝来 Horizontal agarose gel electrophoresis groove of coloured background
CN206339519U (en) * 2017-01-10 2017-07-18 湖南工学院 Agarose gel electrophoresis groove
CN206648993U (en) * 2017-03-06 2017-11-17 中国计量大学 Horizontal electrophoresis tank
CN207882210U (en) * 2018-03-12 2018-09-18 广州奕昕生物科技有限公司 A kind of nucleic acid electrophoresis tank
CN212663511U (en) * 2020-04-10 2021-03-09 新疆大学 Agarose electrophoresis gel preparation facilities
CN212722717U (en) * 2020-07-06 2021-03-16 广州华越肾科再生医学科技有限公司 Agar gel electrophoresis device

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
CN201222053Y (en) * 2008-07-16 2009-04-15 孙朝阳 Disposable electrophoresis rubber-making plate
CN104807873A (en) * 2015-04-17 2015-07-29 贵州大学 Multifunctional agarose gel electrophoresis tank
CN205139079U (en) * 2015-10-30 2016-04-06 满朝来 Horizontal agarose gel electrophoresis groove of coloured background
CN206339519U (en) * 2017-01-10 2017-07-18 湖南工学院 Agarose gel electrophoresis groove
CN206648993U (en) * 2017-03-06 2017-11-17 中国计量大学 Horizontal electrophoresis tank
CN207882210U (en) * 2018-03-12 2018-09-18 广州奕昕生物科技有限公司 A kind of nucleic acid electrophoresis tank
CN212663511U (en) * 2020-04-10 2021-03-09 新疆大学 Agarose electrophoresis gel preparation facilities
CN212722717U (en) * 2020-07-06 2021-03-16 广州华越肾科再生医学科技有限公司 Agar gel electrophoresis device

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