CN114166915A - Horizontal agarose gel electrophoresis sample application color development plate - Google Patents

Horizontal agarose gel electrophoresis sample application color development plate Download PDF

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Publication number
CN114166915A
CN114166915A CN202111482852.5A CN202111482852A CN114166915A CN 114166915 A CN114166915 A CN 114166915A CN 202111482852 A CN202111482852 A CN 202111482852A CN 114166915 A CN114166915 A CN 114166915A
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China
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plate
color development
electrophoresis
sample application
butt joint
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CN202111482852.5A
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CN114166915B (en
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曹雪
刘守振
曹振
刘一华
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Linyi University
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Linyi University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses a horizontal agarose gel electrophoresis sample application color development plate, which relates to the technical field of biology and comprises an electrophoresis box, wherein a butt joint plate is arranged on the top side of the electrophoresis box, and a first installation sleeve is fixedly arranged at the front end of the left side and the rear end of the right side of the butt joint plate. Through the sample application color development board that sets up, the usable self colour of sample application color development board, and then can clearly present sample application hole position, with the efficiency of this improvement sample application, simultaneously through setting up sample application color development board in the bottom side of electrophoresis tank offset plate, can buckle the position bottom of putting at electrophoresis tank offset plate perfectly, and then reach dexterous convenience, do not account for the effect in space, joint through silica gel butt joint and joint groove, can realize being in the same place butt joint board and apron are fixed, and later stage can be very convenient dismantle the apron from the butt joint board, and then when the later stage will make used gel get rid of, can be very convenient take off the apron from the electrophoresis tank.

Description

Horizontal agarose gel electrophoresis sample application color development plate
Technical Field
The invention relates to an agarose gel electrophoresis sample application color development plate, in particular to a horizontal agarose gel electrophoresis sample application color development plate.
Background
Agarose gel electrophoresis is an electrophoresis method using agar or agarose as a support medium. By means of molecular sieve action of agarose gel, nucleic acid fragments are separated according to different molecular weights or molecular shapes and different electrophoretic moving speeds, so that nucleic acid molecules with different molecular weights and different structures can be separated. It is widely used in molecular biology tests such as DNA, RNA, plasmid identification, Polymerase Chain Reaction (PCR), cloning technology, etc. Horizontal agarose gel electrophoresis is one of agarose gel electrophoresis modes, has the advantages of convenient gel preparation and sample addition, simple manufacture, low price and the like, and is widely applied.
At present, the common operation method is to prepare agarose gel by a gel making plate, then take down a comb for making gel, put the gel and the gel making plate into an electrophoresis tank together, and the electrophoresis buffer solution in the electrophoresis tank needs to be submerged in the agarose gel, and then sample application is carried out in a sample application hole on the gel by using a pipette.
The current agarose gel electrophoresis spotting has some defects and shortcomings in the experimental process, and the specific needs for improvement are as follows:
1. when horizontal agarose gel electrophoresis is used for sample application, because agarose gel has high transparency, sample application holes can not be easily found when the agarose gel is put in buffer solution for sample application, thereby causing great trouble to operators, especially beginners;
2. the apron is not convenient for separate with the electrophoresis case, consequently will make used gel get rid of in the later stage, when realizing that the apron is not convenient for and electrophoresis case's reuse, is not convenient for operate.
Disclosure of Invention
The invention aims to provide a horizontal agarose gel electrophoresis sample application color development plate which can be directly buckled at the bottom of a position where an electrophoresis tank rubber plate is placed, is made of silica gel, can be purple, blue and the like, can effectively avoid the phenomenon that the position of a sample application hole is not clear during sample application, and improves the sample application efficiency.
In order to achieve the purpose, the invention provides the following technical scheme: a horizontal agarose gel electrophoresis sample application and color development plate comprises an electrophoresis tank, wherein a butt plate is arranged on the top side of the electrophoresis tank, a first mounting sleeve is fixedly arranged at the front end of the left side and the rear end of the right side of the butt plate, a cover plate is arranged on the upper side of the butt plate, a second mounting sleeve is fixedly arranged at the front end of the left side and the rear end of the right side of the cover plate, an electric pulse butt joint is arranged in the second mounting sleeve, electric pulse pieces are arranged on the left side wall and the right side wall of the electrophoresis tank and are respectively connected with the butt joint pieces in the first mounting sleeves, a concave surface is arranged in the middle of the bottom side wall of the electrophoresis tank, a sample application and color development plate is arranged at the position, corresponding to the concave surface, of the inner wall of the bottom side of the electrophoresis tank, concave surface grooves are formed in the front side and the rear side of the upper surface of the sample application and color development plate, an electrophoresis tank rubber plate is arranged above the sample application and is matched with a comb, the upper surface of apron has seted up square groove, square groove bottom lateral wall has seted up a plurality of spot appearance holes, square groove adaptation has square board.
As a preferred technical scheme of the invention, a plurality of clamping grooves are formed in the upper surface of the butt joint plate close to the edge, and silica gel butt joints are fixedly arranged at the positions of the bottom side of the cover plate corresponding to the clamping grooves.
As a preferred technical scheme of the invention, the clamping grooves are clamped with the corresponding silica gel butt joints, the bottom sides of the clamping grooves are provided with hemispherical grooves, the bottom ends of the silica gel butt joints are provided with hemispherical blocks, and the hemispherical grooves are clamped with the hemispherical blocks.
As a preferable embodiment of the present invention, the spotting and developing plate may be set to purple, blue, or the like.
As a preferred technical scheme of the invention, a plurality of limiting grooves are formed in the bottom side of the sample application color development plate, a limiting block is butted in each limiting groove, and the limiting blocks are fixedly arranged on the positions, corresponding to the concave surfaces, of the inner wall of the bottom side of the electrophoresis tank.
As a preferred technical scheme, vertical grooves are formed in the front side wall and the rear side wall of the electrophoresis tank rubber plate, vertical bars are arranged in the two vertical grooves in a sliding mode, and the two vertical bars are fixedly arranged on the inner walls of the front side and the rear side of the electrophoresis tank respectively.
As a preferred technical scheme of the invention, the electrophoresis tank, the butt joint plate, the cover plate, the electrophoresis tank rubber plate and the square plate are all made of transparent plastic materials.
As a preferable technical scheme of the invention, a sealing plug is fixedly arranged on the side wall of the square plate, and a handle is fixedly arranged on the upper surface of the square plate.
Compared with the prior art, the invention has the beneficial effects that:
1. the sample application color development plate can utilize the color of the sample application color development plate to clearly present the position of the sample application hole so as to improve the sample application efficiency, and meanwhile, the sample application color development plate is arranged at the bottom side of the electrophoresis tank rubber plate and can be perfectly buckled at the bottom of the position where the electrophoresis tank rubber plate is placed, thereby achieving the effects of dexterity, convenience and no space occupation.
2. According to the invention, the butt joint plate and the cover plate can be fixed together by the clamping grooves formed in the butt joint plate and the silica gel butt joints fixedly arranged on the bottom side of the cover plate and the clamping grooves, and the cover plate can be conveniently detached from the butt joint plate in the later period, so that the cover plate can be conveniently taken down from the electrophoresis chamber when used gel is removed in the later period, and the operation is more convenient.
Drawings
FIG. 1 is a schematic illustration of a three-dimensional separation structure according to the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic perspective sectional view of an electrophoresis chamber according to the present invention;
FIG. 4 is a schematic diagram of a three-dimensional structure of an electrophoresis tank slab rubber of the present invention;
FIG. 5 is a schematic perspective view of a dot color display panel according to the present invention.
In the figure: the electrophoresis apparatus comprises an electrophoresis tank 1, a butt joint plate 2, an installation sleeve I3, a cover plate 4, an installation sleeve II 5, a clamping groove 6, a silica gel butt joint 7, an electric pulse butt joint 8, an electric pulse piece 9, a concave surface 10, a sample application color development plate 11, a limiting block 12, a limiting groove 13, a concave surface groove 14, an electrophoresis groove rubber plate 15, a comb 16, a vertical groove 17, a vertical strip 18, a square groove 19, a sample application hole 20, a square plate 21 and a sealing plug 22.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a horizontal agarose gel electrophoresis sample application color development plate, which comprises an electrophoresis tank 1, wherein a docking plate 2 is arranged on the top side of the electrophoresis tank 1, a first mounting sleeve 3 is fixedly arranged on each of the front left end and the rear right end of the docking plate 2, a cover plate 4 is arranged on the upper side of the docking plate 2, a second mounting sleeve 5 is fixedly arranged on each of the front left end and the rear right end of the cover plate 4, a second electrical pulse butt joint 8 is arranged in the second mounting sleeve 5, the first mounting sleeve 3 and the second mounting sleeve 5 can be used for butt joint of the electrical pulse butt joint 8, electrical pulse sheets 9 are arranged on each of the left side wall and the right side wall of the electrophoresis tank 1, the two electrical pulse sheets 9 are respectively connected with the butt joint sheets in the first mounting sleeves 3, a concave surface 10 is arranged in the middle of the bottom side wall of the electrophoresis tank 1, a sample application color development plate 11 is arranged on the position, corresponding to the concave surface 10, concave surface grooves 14 are arranged on each of the front side and the front side of the upper surface of the sample application color development plate 11, an electrophoresis tank rubber plate 15 is arranged above the sample application color development plate 11, the electrophoresis tank rubber plate 15 is matched with a comb 16, the upper surface of the cover plate 4 is provided with a square groove 19, the bottom side wall of the square groove 19 is provided with a plurality of sample application holes 20, the square groove 19 is matched with a square plate 21, the sample application color development plate 11 is placed on the concave surface 10 of the inner wall of the electrophoresis tank 1, a plurality of limit blocks 12 and a plurality of limit grooves 13 are butted together, then electrophoresis buffer solution is added into the electrophoresis tank 1, agarose gel is prepared through the electrophoresis tank rubber plate 15, then the comb 16 for preparing gel is taken down, the agarose gel and the electrophoresis tank rubber plate 15 are placed into the middle part of the electrophoresis tank 1, meanwhile, the vertical groove 17 and the vertical bar 18 are clamped together, the electrophoresis tank rubber plate 15 can be prevented from moving in the electrophoresis tank 1, then the cover plate 4 is covered on the butt plate 2, and a plurality of silica gel butt joints 7 are clamped into a plurality of clamping grooves 6, the cover plate 4 is firmly fixed on the electrophoresis tank 1, after the electric pulse butt joint 8 is connected, the square plate 21 can be taken out from the square groove 19, then the sample application operation is carried out through the sample application hole 20, a plurality of clamping grooves 6 are arranged on the upper surface of the butt joint plate 2 near the edge, the positions of the bottom side of the cover plate 4 corresponding to the clamping grooves 6 are fixedly provided with silica gel butt joints 7, the clamping grooves 6 are clamped with the corresponding silica gel butt joints 7, the bottom side of the clamping grooves 6 is provided with a semi-spherical groove, the bottom end of the silica gel butt joint 7 is provided with a semi-spherical block, the semi-spherical groove is clamped with the semi-spherical block, the silica gel butt joint 7 can be prevented from easily separating from the clamping grooves 6 by clamping the arranged semi-spherical groove with the semi-spherical block, the sample application color development plate 11 can be set to be purple, blue and the like, the bottom side of the sample application color development plate 11 is provided with a plurality of limiting grooves 13, each limiting groove 13 is internally butted with a limiting block 12, a plurality of limiting blocks 12 are fixedly arranged on the positions of the inner wall of the bottom side of the electrophoresis tank 1 corresponding to the concave surfaces 10, the sample application color development plate 11 can be prevented from moving in the electrophoresis tank 1 by the clamping of the limiting grooves 13 and the limiting blocks 12, the front side wall and the rear side wall of the electrophoresis tank rubber plate 15 are respectively provided with a vertical groove 17, vertical strips 18 are respectively arranged in the two vertical grooves 17 in a sliding manner, the two vertical strips 18 are respectively fixedly arranged on the inner walls of the front side and the rear side of the electrophoresis tank 1, the displacement of the electrophoresis tank rubber plate 15 in the electrophoresis tank 1 can be prevented by the clamping of the vertical strips 18 and the vertical grooves 17, the electrophoresis tank 1, the butt joint plate 2, the cover plate 4, the electrophoresis tank rubber plate 15 and the square plate 21 are all made of transparent plastic materials, the transparent plastic materials are convenient for observation in dispensing, a sealing plug 22 is fixedly arranged on the side wall of the square plate 21, and a handle is fixedly arranged on the upper surface of the square plate 21, through the sealing plug 22 that sets up, can guarantee that square board 21 can be firm fixes in square groove 19, be convenient for take out square board 21 from square groove 19 through the handle that sets up.
When the electrophoresis applying and developing device is used specifically, the sample applying and developing plate 11 is placed on the concave surface 10 of the inner wall of the electrophoresis box 1, the limit blocks 12 and the limit grooves 13 are butted together, then an electrophoresis buffer solution is added into the electrophoresis box 1, an agarose gel is prepared through the electrophoresis groove rubber plate 15, then the comb 16 for preparing gel is taken down, the agarose gel and the electrophoresis groove rubber plate 15 are placed into the middle of the electrophoresis box 1, meanwhile, the vertical grooves 17 and the vertical bars 18 are clamped together, the electrophoresis groove rubber plate 15 is prevented from moving in the electrophoresis box 1, then the cover plate 4 is covered on the butt joint plate 2, meanwhile, the silica gel butt joints 7 are clamped into the clamping grooves 6, the cover plate 4 is firmly fixed on the electrophoresis box 1, after the electric pulse butt joint 8 is switched on, the square plate 21 can be taken out of the square groove 19 at the moment, and then sample applying operation is carried out through the sample applying hole 20.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides a horizontal agarose gel electrophoresis sample application color development board, includes electrophoresis tank (1), its characterized in that: the electrophoresis tank is characterized in that a butt joint plate (2) is arranged on the top side of the electrophoresis tank (1), a first mounting sleeve (3) is fixedly arranged at the front left end and the rear right end of the butt joint plate (2), a cover plate (4) is arranged on the upper side of the butt joint plate (2), a second mounting sleeve (5) is fixedly arranged at the front left end and the rear right end of the cover plate (4), an electric pulse butt joint (8) is arranged in the second mounting sleeve (5), electric pulse sheets (9) are arranged on the left side wall and the right side wall of the electrophoresis tank (1), the two electric pulse sheets (9) are respectively connected with butt joint sheets in the first mounting sleeves (3), a concave surface (10) is arranged in the middle of the bottom side wall of the electrophoresis tank (1), a sample application plate (11) is arranged at a position corresponding to the color development plate (10) on the inner bottom side wall of the electrophoresis tank (1), and concave surface grooves (14) are formed in the front and rear sides of the upper surface of the sample application plate (11), an electrophoresis tank rubber plate (15) is arranged above the sample application color development plate (11), the electrophoresis tank rubber plate (15) is matched with a comb (16), a square groove (19) is formed in the upper surface of the cover plate (4), a plurality of sample holes (20) are formed in the bottom side wall of the square groove (19), and the square groove (19) is matched with a square plate (21).
2. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: the upper surface of butt joint board (2) is close to border department and has seted up a plurality of joint grooves (6), the bottom side of apron (4) all is fixed with silica gel butt joint (7) with the position that a plurality of joint grooves (6) correspond.
3. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 2, characterized in that: a plurality of joint groove (6) and corresponding silica gel butt joint (7) joint, and the bottom side in joint groove (6) is provided with the hemisphere shape groove, and the bottom of silica gel butt joint (7) is provided with the hemisphere shape piece, and hemisphere shape groove and hemisphere shape piece joint.
4. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: the sample application and color development plate (11) can be set to be purple, blue and the like.
5. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: the sample application and color development plate is characterized in that a plurality of limiting grooves (13) are formed in the bottom side of the sample application and color development plate (11), limiting blocks (12) are butted in each limiting groove (13), and the limiting blocks (12) are fixedly arranged on the positions, corresponding to the concave surfaces (10), of the inner wall of the bottom side of the electrophoresis tank (1).
6. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: vertical grooves (17) are formed in the front side wall and the rear side wall of the electrophoresis tank rubber plate (15), vertical bars (18) are arranged in the vertical grooves (17) in a sliding mode, and the two vertical bars (18) are fixedly arranged on the inner walls of the front side and the rear side of the electrophoresis tank (1) respectively.
7. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: electrophoresis case (1), butt joint board (2), apron (4), electrophoresis tank offset plate (15) and square board (21) are the transparent plastic material.
8. A horizontal agarose gel electrophoresis spotting and color development plate according to claim 1, characterized in that: the side wall of the square plate (21) is fixedly provided with a sealing plug (22), and the upper surface of the square plate (21) is fixedly provided with a handle.
CN202111482852.5A 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate Active CN114166915B (en)

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Application Number Priority Date Filing Date Title
CN202111482852.5A CN114166915B (en) 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate

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Application Number Priority Date Filing Date Title
CN202111482852.5A CN114166915B (en) 2021-12-07 2021-12-07 Horizontal agarose gel electrophoresis sample application color plate

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CN114166915A true CN114166915A (en) 2022-03-11
CN114166915B CN114166915B (en) 2023-12-01

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
CN201222053Y (en) * 2008-07-16 2009-04-15 孙朝阳 Disposable electrophoresis rubber-making plate
CN104807873A (en) * 2015-04-17 2015-07-29 贵州大学 Multifunctional agarose gel electrophoresis tank
CN205139079U (en) * 2015-10-30 2016-04-06 满朝来 Horizontal agarose gel electrophoresis groove of coloured background
CN206339519U (en) * 2017-01-10 2017-07-18 湖南工学院 Agarose gel electrophoresis groove
CN206648993U (en) * 2017-03-06 2017-11-17 中国计量大学 Horizontal electrophoresis tank
CN207882210U (en) * 2018-03-12 2018-09-18 广州奕昕生物科技有限公司 A kind of nucleic acid electrophoresis tank
CN212663511U (en) * 2020-04-10 2021-03-09 新疆大学 Agarose electrophoresis gel preparation facilities
CN212722717U (en) * 2020-07-06 2021-03-16 广州华越肾科再生医学科技有限公司 Agar gel electrophoresis device

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
US5843295A (en) * 1996-12-19 1998-12-01 Pharmacia Biotech Gel electrophoresis well-forming and loading-guide comb
CN201222053Y (en) * 2008-07-16 2009-04-15 孙朝阳 Disposable electrophoresis rubber-making plate
CN104807873A (en) * 2015-04-17 2015-07-29 贵州大学 Multifunctional agarose gel electrophoresis tank
CN205139079U (en) * 2015-10-30 2016-04-06 满朝来 Horizontal agarose gel electrophoresis groove of coloured background
CN206339519U (en) * 2017-01-10 2017-07-18 湖南工学院 Agarose gel electrophoresis groove
CN206648993U (en) * 2017-03-06 2017-11-17 中国计量大学 Horizontal electrophoresis tank
CN207882210U (en) * 2018-03-12 2018-09-18 广州奕昕生物科技有限公司 A kind of nucleic acid electrophoresis tank
CN212663511U (en) * 2020-04-10 2021-03-09 新疆大学 Agarose electrophoresis gel preparation facilities
CN212722717U (en) * 2020-07-06 2021-03-16 广州华越肾科再生医学科技有限公司 Agar gel electrophoresis device

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