CN210109022U - Protein electrophoresis apparatus - Google Patents
Protein electrophoresis apparatus Download PDFInfo
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- CN210109022U CN210109022U CN201920624335.9U CN201920624335U CN210109022U CN 210109022 U CN210109022 U CN 210109022U CN 201920624335 U CN201920624335 U CN 201920624335U CN 210109022 U CN210109022 U CN 210109022U
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- electrode plug
- negative electrode
- positive electrode
- electrophoresis
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Abstract
The utility model relates to a protein electrophoresis apparatus, which comprises an electrophoresis tank, a rubber clamping frame, an upper cover, a positive electrode plug and a negative electrode plug, wherein the positive electrode plug and the negative electrode plug are used for being connected with an external power supply and supplying power to an electrophoresis test; the outer rubber clamping frame is arranged in the electrophoresis tank; a positive electrode plug and a negative electrode plug are arranged on the rubber clamping frame; the electrophoresis tank is characterized by further comprising an electricity-proof isolation net, wherein frame body placing holes used for placing the included angle frame and the outer rubber clamping frame are formed in the electricity-proof isolation net, and the electricity-proof isolation net is covered on the electrophoresis tank. Through set up anticreep partition net on the electrophoresis tank, make protein electrophoresis apparatus, can reduce the risk that the electric leakage accident caused to the experimenter.
Description
Technical Field
The utility model relates to a protein experimental apparatus field, in particular to protein electrophoresis apparatus.
Background
Protein Electrophoresis (SPE) is an analytical technique for proteins that are negatively or positively charged in a buffer and move to the anode in an electric field called electrophoresis, and different protein molecules have different electrophoretic mobilities. The protein electrophoresis apparatus is used for SDS-PAGE gel electrophoresis, and is aimed at separating protein mixture into different bands on the gel according to the size of protein, and then the correspondent bands on the gel can be recovered to obtain correspondent protein, or the gel can be directly used for making immunological analysis of protein. The existing protein electrophoresis apparatus comprises an electrophoresis tank, a rubber clamping frame, an outer rubber clamping frame and an upper cover; the outer rubber clamping frame is arranged in the electrophoresis tank; the rubber clamping frame is provided with a positive electrode plug and a negative electrode plug. In the electrophoresis test process, the electrophoresis liquid can be electrified, so when a tester takes the rubber clamping frame or the outer rubber clamping frame, if the operation is careless, the danger of electric leakage is easy to occur.
Disclosure of Invention
The utility model aims at overcoming the problem that prior art exists, providing a protein electrophoresis apparatus, can reduce the danger of the causing of electric leakage.
In order to solve the above problems, the utility model adopts the following technical scheme:
a protein electrophoresis apparatus is characterized by comprising an electrophoresis tank, a rubber clamping frame, an upper cover, a positive electrode plug and a negative electrode plug, wherein the positive electrode plug and the negative electrode plug are used for being connected with an external power supply and supplying power to an electrophoresis test; the outer rubber clamping frame is arranged in the electrophoresis tank; a positive electrode plug and a negative electrode plug are arranged on the rubber clamping frame; the electrophoresis tank is characterized by further comprising an electricity-proof isolation net, wherein frame body placing holes used for placing the included angle frame and the outer rubber clamping frame are formed in the electricity-proof isolation net, and the electricity-proof isolation net is covered on the electrophoresis tank.
Preferably, the electrophoresis tank comprises at least two rubber clamping frames, wherein each rubber clamping angle frame comprises a clamping groove arranged on the inner wall of the electrophoresis tank;
the battery also comprises a negative electrode conductive part and a positive electrode conductive part;
the positive electrode conductive part comprises an electrode part and a lead part, the electrode part is arranged at the top of the electrophoresis tank, the lead part is connected with the electrode part and extends to the lower part of the inner wall of the electrophoresis tank, and the lead part is connected with the clamping tank; the electricity
The positive electrode plug is arranged on the pole part and connected with a lead, the lead is hidden in the negative electrode conductive part and extends along the lead part, and the free end of the lead extends into the clamping groove;
the negative electrode conductive part comprises an electrode part which is arranged at the top of the electrophoresis tank; the electrode part is provided with the negative electrode plug, the negative electrode is connected with a conductive block, and the free end of the conductive block protrudes out of the electrode part;
and the power supply end of the upper cover, which is used for supplying power to the positive electrode plug and the negative electrode plug, is matched with the positive electrode plug and the negative electrode plug.
Preferably, the electrophoresis tank further comprises an outer rubber clamping frame, and the outer rubber clamping frame is arranged in the electrophoresis tank; the positive electrode plug and the negative electrode plug are arranged on the rubber clamping frame.
Preferably, the battery also comprises an electrode sleeve which is sleeved on the positive electrode plug or the negative electrode plug.
Preferably, the device further comprises a prefabricated film, wherein the prefabricated film comprises a film body and a sample feeder arranged on the film body.
Preferably, the sample adding device comprises a body, wherein a plurality of adding channels are arranged on the body, and the number of the adding channels is the same as that of the sample cavities on the body; the sample feeder and the film body are integrally formed, and a groove for peeling the sample feeder off the film body is arranged between the sample feeder and the film body; the body is peelably arranged on the film body.
Preferably, the electrophoresis device also comprises a slide glass for manufacturing the protein electrophoresis film, and a convex strip which is used as a reference line for keeping the gel liquid level at the highest position and horizontal is arranged on the outer side of the slide glass.
Preferably, the surface of the convex strip is a frosted surface.
Preferably, the positive electrode plug and the negative electrode plug are both arranged as truncated cone-shaped conductive blocks, and the power supply part connected with the positive electrode plug and the negative electrode plug on the inner side of the upper cover is arranged as a groove-shaped power supply part; the circular truncated cone-shaped conductive block is matched with the groove-shaped power supply part.
Preferably, the inner wall of the electrophoresis tank is provided with a plurality of prefabricated film clamping grooves.
Compared with the prior art the beneficial effects of the utility model:
through set up anticreep partition net on the electrophoresis tank, make protein electrophoresis apparatus, can reduce the risk that the electric leakage accident caused to the experimenter. The electrode sleeves are respectively sleeved on the positive electrode and the negative electrode, so that the danger of electric leakage accidents can be further avoided. Set up detachable sample adder on prefabricated offset plate to make the sample add more convenient and fast, can avoid some unnecessary samples to remain in the sample chamber moreover, influence the experiment effect. Through set up the sand grip on prefabricated film, can regard as the reference line of gel liquid to make the experimenter add gel liquid and can have the reference line, so that the gel liquid of adding can use the reference line as the benchmark, keeps the level as far as possible.
Drawings
Figure 1 is the structure schematic diagram of the electricity-proof screen of the present invention.
Fig. 2 is a schematic diagram of the prefabricated film of the present invention.
Fig. 3 is a schematic view of the adhesive frame and the electrode sheath according to embodiment 1.
FIG. 4 is a schematic view of the protein electrophoresis apparatus described in example 1 (the electricity-proof screen is omitted, and the electrode sheath needs to be removed when the cover is closed).
FIG. 5 is a schematic view of a slide according to the present invention.
FIG. 6 is a schematic diagram of the protein electrophoresis apparatus of example 2.
In the figure:
1-electrophoresis tank; 2-anti-electric insulation net; 3, prefabricating a film; 31-a sample chamber; 32-a groove; 4-sample feeder; 41-an addition channel; 5, convex strips; 6, clamping a rubber frame; 7, electrode sleeve;
8, covering the upper cover; 91-positive electrode plug; 92-negative electrode plug; 10-outer rubber frame; 11-slide; 111-sample chamber; 12-supply terminal lead; 13-outer laminated frame; 01-clamping groove; 02 — positive conductive part; 03 — negative conductive part; 031-electrode parts; 032 — a lead portion; 04-a conducting wire; 05-conductive block.
Detailed Description
Meanwhile, the present invention will be further explained with reference to the drawings and the following detailed description.
Example 1
As shown in fig. 1, the protein electrophoresis apparatus of the present invention comprises an electrophoresis tank, a rubber clamping frame for placing a prefabricated rubber sheet, an outer rubber clamping frame and an upper cover; the outer rubber clamping frame is arranged in the electrophoresis tank; the gel-sandwiched frame is provided with a positive electrode plug and a negative electrode plug which are connection plugs used for being connected with an external power supply and supplying power to the electrophoresis test; the electrophoresis tank is characterized by further comprising an electricity-proof isolation net, wherein frame body placing holes used for placing the glue clamping frame and the outer glue clamping frame are formed in the electricity-proof isolation net, and an electricity-proof isolation net cover is arranged on the electrophoresis tank. The upper cover is positioned on the electric isolation net and covers the electrophoresis tank.
The two electrode sleeves are respectively sleeved on the positive electrode plug and the negative electrode plug when the upper cover is not covered. When the upper cover needs to be covered, the two electrode sleeves are taken down and then covered with the upper cover.
The positive electrode plug and the negative electrode plug can be both arranged into a truncated cone-shaped conductive block, and the power supply part connected with the positive electrode plug and the negative electrode plug on the inner side of the upper cover is arranged into a groove-shaped power supply part; the circular truncated cone-shaped conductive block is matched with the groove-shaped power supply part.
Still include the prefabricated film, the prefabricated film includes the film body and sets up the sample adder on the film body. The body comprises two layers of outer glass sheets and a gel sheet positioned between the two layers of outer glass sheets.
The sample adding device comprises a body, wherein a plurality of adding channels are arranged on the body, and the number of the adding channels is the same as that of the sample cavities on the body. The sample feeder and the film body are integrally formed, and a groove for conveniently peeling the sample feeder from the film body is arranged between the sample feeder and the film body; the body is arranged on the film body in a strippable manner.
The gel liquid level monitoring device further comprises a glass slide, wherein convex strips used as reference lines for keeping the gel liquid level at the highest position are arranged on the outer side of the glass slide, and the surfaces of the convex strips are frosted surfaces.
Through set up anticreep and separate the net on the electrophoresis tank, if the electrophoresis apparatus electric leakage problem appears, when leading to the electrophoresis liquid to be electrified, owing to have the separation that anticreep separated the net, make the experimenter can not put into the electrophoresis liquid by the handle mistake, avoided the danger of electrocuteeing. Reduce the risk that the electric leakage accident caused to the experimenter. The electrode sleeves are respectively sleeved on the positive electrode and the negative electrode, so that the danger of electric leakage accidents can be further avoided. The inner wall of the electrophoresis tank is provided with a plurality of prefabricated film clamping grooves.
Set up detachable sample adder on prefabricated offset plate, the pipettor can add the sample into the sample adder in, articles for use fall into the sample chamber from the sample adder to make the sample add more convenient and fast, can avoid some unnecessary samples to remain in the wall in sample chamber moreover, influence the experiment effect.
The convex strips arranged on the slide are used as a reference line of the gel liquid, so that an experimenter can have a reference position when adding the gel liquid, and the added gel liquid can be kept horizontal as much as possible by taking the reference line as a reference. So that the experimenter unskilled in operation can also successfully operate.
Example 2
As shown in fig. 1, the protein electrophoresis apparatus of the present invention comprises an electrophoresis tank, a gel-clamping frame and an upper cover; the glue clamping frame is arranged in the electrophoresis tank; the electrophoresis tank is characterized by further comprising an electricity-proof isolation net, wherein a frame body placing hole used for placing the rubber clamping frame is formed in the electricity-proof isolation net, and an electricity-proof isolation net cover is arranged on the electrophoresis tank. The upper cover is positioned on the electric isolation net and covers the electrophoresis tank.
The protein electrophoresis apparatus comprises at least two rubber clamping frames, wherein the rubber clamping frames comprise clamping grooves arranged on the inner wall of the electrophoresis groove.
Also includes a negative electrode conductive part and a positive electrode conductive part.
The positive electrode conductive part comprises an electrode part and a lead part, the electrode part is arranged at the top of the electrophoresis tank, the lead part is connected with the electrode part and extends to the lower part of the inner wall of the electrophoresis tank, and the lead part is connected with the clamping tank; the electrode part is provided with a positive electrode plug which is connected with a lead, the lead is hidden in the negative conductive part and extends along the lead part, and the free end of the lead extends into the clamping groove. The negative electrode conducting part comprises an electrode part which is arranged at the top of the electrophoresis tank; the electrode part is provided with a negative electrode plug, the negative electrode is connected with the conductive block, and the free end of the conductive block protrudes out of the electrode part. The positive electrode plug and the negative electrode plug are used for being connected with an external power supply and supplying power to the electrophoresis test.
The inner side of the upper cover is provided with a power supply end used for supplying power to the positive electrode plug and the negative electrode plug, and the power supply end is matched with the positive electrode plug and the negative electrode plug.
The two electrode sleeves are respectively sleeved on the positive electrode plug and the negative electrode plug when the upper cover is not covered; when the upper cover needs to be covered, the two electrode sleeves are taken down and then covered with the upper cover.
Still include the prefabricated film, the prefabricated film includes the film body and sets up the sample adder on the film body. The body comprises two layers of outer glass sheets and a gel sheet positioned between the two layers of outer glass sheets.
The sample adding device comprises a body, wherein a plurality of adding channels are arranged on the body, and the number of the adding channels is the same as that of the sample cavities on the body. The sample feeder and the film body are integrally formed, and a groove for conveniently peeling the sample feeder from the film body is arranged between the sample feeder and the film body; the body is arranged on the film body in a strippable manner.
In this embodiment, after the upper cover is covered on the electrophoresis tank, the two power supply terminals are electrically connected with the positive electrode plug and the negative electrode plug respectively, and the current forms a conducting circuit through the zheng electrode plug, the conducting wire, the electrophoresis solution, the conducting block and the negative electrode plug, thereby realizing electrophoresis of the electrophoresis solution and enabling the protein electrophoresis test to be carried out.
Through set up anticreep and separate the net on the electrophoresis tank, if the electrophoresis apparatus electric leakage problem appears, when leading to the electrophoresis liquid to be electrified, owing to have the separation that anticreep separated the net, make the experimenter can not put into the electrophoresis liquid by the handle mistake, avoided the danger of electrocuteeing. Reduce the risk that the electric leakage accident caused to the experimenter. The electrode sleeves are respectively sleeved on the positive electrode and the negative electrode, so that the danger of electric leakage accidents can be further avoided.
Set up detachable sample adder on prefabricated offset plate, the pipettor can add the sample into the sample adder in, articles for use fall into the sample chamber from the sample adder to make the sample add more convenient and fast, can avoid some unnecessary samples to remain in the wall in sample chamber moreover, influence the experiment effect.
While the preferred embodiments of the present invention have been illustrated and described in detail, it should be understood that modifications and variations can be effected by one skilled in the art in light of the above teachings without undue experimentation. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning or limited experiments based on the present invention concept should be within the scope of the present claims.
Claims (10)
1. A protein electrophoresis apparatus is characterized by comprising an electrophoresis tank, a rubber clamping frame, an upper cover, a positive electrode plug and a negative electrode plug, wherein the positive electrode plug and the negative electrode plug are used for being connected with an external power supply and supplying power to an electrophoresis test; the outer rubber clamping frame is arranged in the electrophoresis tank; a positive electrode plug and a negative electrode plug are arranged on the rubber clamping frame; the electrophoresis tank is characterized by further comprising an electricity-proof isolation net, wherein frame body placing holes used for placing the included angle frame and the outer rubber clamping frame are formed in the electricity-proof isolation net, and the electricity-proof isolation net is covered on the electrophoresis tank.
2. The protein electrophoresis apparatus according to claim 1, comprising at least two glue clamping frames, wherein the glue clamping angle frame comprises a clamping groove arranged on the inner wall of the electrophoresis tank;
the battery also comprises a negative electrode conductive part and a positive electrode conductive part;
the positive electrode conductive part comprises an electrode part and a lead part, the electrode part is arranged at the top of the electrophoresis tank, the lead part is connected with the electrode part and extends to the lower part of the inner wall of the electrophoresis tank, and the lead part is connected with the clamping tank; the electrode part is provided with the positive electrode plug, the positive electrode plug is connected with a lead, the lead is hidden in the negative conductive part, extends along the lead part and the free end of the lead extends into the clamping groove;
the negative electrode conductive part comprises an electrode part which is arranged at the top of the electrophoresis tank; the electrode part is provided with the negative electrode plug, the negative electrode is connected with a conductive block, and the free end of the conductive block protrudes out of the electrode part;
and the power supply end of the upper cover, which is used for supplying power to the positive electrode plug and the negative electrode plug, is matched with the positive electrode plug and the negative electrode plug.
3. The protein electrophoresis apparatus according to claim 1 or 2, further comprising an outer gel frame disposed within the electrophoresis tank; the positive electrode plug and the negative electrode plug are arranged on the rubber clamping frame.
4. The protein electrophoresis apparatus of claim 3 further comprising an electrode sleeve configured to fit over the positive electrode plug or the negative electrode plug.
5. The protein electrophoresis apparatus according to claim 1, further comprising a pre-fabricated film, wherein the pre-fabricated film comprises a film body and a sample feeder disposed on the film body.
6. The protein electrophoresis apparatus according to claim 5, wherein the sample adding device comprises a body, a plurality of adding channels are arranged on the body, and the number of the adding channels is the same as that of the sample cavities on the body; the sample feeder and the film body are integrally formed, and a groove for peeling the sample feeder off the film body is arranged between the sample feeder and the film body; the body is peelably arranged on the film body.
7. The protein electrophoresis apparatus according to claim 1, further comprising a slide for making a protein electrophoresis film, wherein the outer side of the slide is provided with a convex strip for serving as a reference line for keeping the gel level at the highest position and horizontal.
8. The protein electrophoresis apparatus of claim 7 wherein the surface of said ribs is a frosted surface.
9. The protein electrophoresis apparatus according to claim 3, wherein the positive electrode plug and the negative electrode plug are both provided as truncated cone-shaped conductive blocks, and the power supply parts connected with the positive electrode plug and the negative electrode plug on the inner side of the upper cover are provided as groove-shaped power supply parts; the circular truncated cone-shaped conductive block is matched with the groove-shaped power supply part.
10. The protein electrophoresis apparatus according to claim 3, wherein the inner wall of said electrophoresis tank is provided with a plurality of pre-fabricated film clamping grooves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920624335.9U CN210109022U (en) | 2019-04-30 | 2019-04-30 | Protein electrophoresis apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920624335.9U CN210109022U (en) | 2019-04-30 | 2019-04-30 | Protein electrophoresis apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210109022U true CN210109022U (en) | 2020-02-21 |
Family
ID=69540066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920624335.9U Expired - Fee Related CN210109022U (en) | 2019-04-30 | 2019-04-30 | Protein electrophoresis apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210109022U (en) |
-
2019
- 2019-04-30 CN CN201920624335.9U patent/CN210109022U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200221 |
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| CF01 | Termination of patent right due to non-payment of annual fee |