CN114149929A - Process technology of compound microbial agent for apple planting - Google Patents
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- 238000000034 method Methods 0.000 title claims abstract description 37
- 230000000813 microbial effect Effects 0.000 title claims abstract description 25
- 238000005516 engineering process Methods 0.000 title claims abstract description 20
- 150000001875 compounds Chemical class 0.000 title claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 49
- 238000000855 fermentation Methods 0.000 claims abstract description 34
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 239000002054 inoculum Substances 0.000 claims abstract description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 6
- 230000000443 biocontrol Effects 0.000 claims abstract description 6
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 6
- 239000004310 lactic acid Substances 0.000 claims abstract description 6
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 41
- 238000003756 stirring Methods 0.000 claims description 26
- 238000003860 storage Methods 0.000 claims description 19
- 238000011218 seed culture Methods 0.000 claims description 18
- 230000004913 activation Effects 0.000 claims description 15
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 239000004615 ingredient Substances 0.000 claims description 12
- 241000233866 Fungi Species 0.000 claims description 10
- 238000000746 purification Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 238000007710 freezing Methods 0.000 claims description 7
- 230000008014 freezing Effects 0.000 claims description 7
- 230000001360 synchronised effect Effects 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 241000186361 Actinobacteria <class> Species 0.000 claims description 5
- 230000003213 activating effect Effects 0.000 claims description 5
- 238000005138 cryopreservation Methods 0.000 claims description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 5
- 238000004891 communication Methods 0.000 abstract description 3
- 241001446247 uncultured actinomycete Species 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 241000693079 Maloideae Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention belongs to the field of biofertilizer, in particular to a process technology of a compound microbial inoculant for apple planting, aiming at the problems that various reagents are required to be sequentially measured in the preparation process of the existing compound microbial inoculant, the reagents cannot be synchronously carried out side by side, the preparation efficiency is low, data are not communicated and compared with each other, and the processing efficiency is low, the following scheme is proposed, and the process technology comprises the following steps: s1: preparing raw materials: 15-30 parts of photosynthetic bacteria group, 10-20 parts of lactic acid bacteria group, 15-25 parts of gram-positive actinomycete group, 10-25 parts of fermentation system filamentous bacteria group, 10-25 parts of yeast bacteria group, 5-10 parts of biocontrol bacteria, 5-10 parts of silicate bacteria and 1-5 parts of yield-increasing bacteria. The invention does not need to prepare a plurality of reagents in sequence, can be synchronously carried out side by side, has higher preparation efficiency, and has the advantages of mutual communication and comparison of data and higher processing efficiency.
Description
Technical Field
The invention relates to the technical field of biofertilizers, in particular to a process technology of a compound microbial agent for apple planting.
Background
Apple is an important crop in China, is one of fruits, is a plant of Maloideae, Rosaceae, and is a fallen leaf arbor, the fruit of apple is rich in minerals and vitamins, and is one of the fruits frequently eaten by people, and is the second fruit in time yield, but the apple fertilization technology and the pest control technology still have many difficulties to be solved urgently, most of the existing apple diseases can be prevented and controlled by chemical agents, although the apple diseases can play a certain role, the long-term application of a large amount of chemical agents can easily cause environmental pollution and drug residue of apple, the biological control is widely concerned by researchers at home and abroad due to the safety of the biological control on environment, ecology, human and livestock, and the apple is mainly characterized in that the growth of pathogenic bacteria or the self-resistance of plants is improved by applying antagonistic bacteria, organic fertilizers or biological organic fertilizers and other measures to adjust the soil micro-ecology, therefore, the occurrence of soil-borne diseases is inhibited, however, the existing compound microbial inoculant needs to sequentially measure various reagents in the preparation process, cannot be synchronously carried out side by side, has low preparation efficiency, does not have mutual communication and comparison of data, and has low processing efficiency.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a process technology of a compound microbial agent for apple planting.
In order to achieve the purpose, the invention adopts the following technical scheme:
a process technology of a compound microbial agent for apple planting comprises the following steps:
s1: preparing raw materials: 15-30 parts of photosynthetic bacteria, 10-20 parts of lactic acid bacteria, 15-25 parts of gram-positive actinomycetes, 10-25 parts of fermentation system filamentous bacteria, 10-25 parts of yeast bacteria, 5-10 parts of biocontrol bacteria, 5-10 parts of silicate bacteria and 1-5 parts of yield-increasing bacteria;
s2: observation characteristics: identifying the purity of flora in the raw materials, checking the production performance, checking whether the flora is polluted by other bacteria or not, observing the activity of the strains, selecting the flora with higher integrity, and putting the flora raw materials into a storage tube in sequence for freezing storage;
s3: and (3) purification: activating the flora in the cryopreservation tube in a slant, and purifying in a flat plate to obtain a strain slant;
s4: and (4) classification: classifying, counting and recording a plurality of flora slopes, preparing for synchronous culture, recording classified and counted information, and uploading recorded information in real time;
s5: fermentation culture: inoculating the purified flora slant into a seed culture medium bottle, placing the seed culture medium bottle into a shaking table for culture, simultaneously injecting air into the seed culture medium bottle by using an air compressor in the culture process, installing an air filter in the air circulation process, and sending sterile air into a seed tank;
s6: recording and uploading data on the seed tanks which are classified for culture, putting cultured flora into a main fermentation tank for secondary fermentation, putting culture medium raw materials and culture medium ingredients into a culture medium ingredient tank for mixing, respectively sending the mixed culture medium into a plurality of main fermentation tanks, and carrying out steam sterilization in the conveying process;
s7: the fungus crowd after will fermenting is put into the storage tank and is cooled off, puts into the agitator with multiple fungus crowd and mixes the stirring, adjusts pH value simultaneously, and the inside humidity state of real-time supervision when the stirring is carried dry air in to the agitator, dries it when the stirring is mixed, stops drying and stirring after reaching appointed numerical value with inside microbial inoculum humidity, takes out microbial composite inoculum and unifies the packing.
Specifically, in S3, the activation temperature is 36.5-37.2 ℃, and the activation time is 21-25 h.
Specifically, in the S3, the purification temperature is 36.8-37.5 ℃, and the activation time is 23-27 h.
Specifically, in S5, the rotation speed of the shaking table is 130-180r/h, the set temperature of the shaking table is 36.8-37.2 ℃, and the working time of the shaking table is 16-20 h.
Specifically, in the S6, the fermentation time is 12-16h, and the pH value is 7.0-7.3.
Specifically, in the S7, the cooling time is 3-6h, and the pH value is 7.0-7.5.
Compared with the prior art, the invention has the beneficial effects that:
(1) the process technology of the compound microbial agent for apple planting provided by the invention adopts classification and synchronous preparation, has higher efficiency and better data intuition, and can upload data in real time and monitor and analyze the data.
(2) According to the process technology of the compound microbial agent for apple planting, multiple agents do not need to be prepared in sequence, the preparation efficiency is high after the multiple agents are synchronously prepared side by side, data are communicated and compared with each other, and the processing efficiency is high.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be understood that the drawings in the following description are illustrative only, and that the structures, proportions, sizes, and other elements shown in the drawings are incorporated herein by reference in their entirety for all purposes in the present disclosure, which are not intended to limit the scope of the invention, which is to be construed as limiting the invention in any manner, and not necessarily for all purposes, except as technically essential.
FIG. 1 is a flow chart of a process technology of a compound microbial agent for apple planting according to the present invention.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1, a process technology of a compound microbial agent for apple planting comprises the following steps:
s1: preparing raw materials: 15-30 parts of photosynthetic bacteria, 10-20 parts of lactic acid bacteria, 15-25 parts of gram-positive actinomycetes, 10-25 parts of fermentation system filamentous bacteria, 10-25 parts of yeast bacteria, 5-10 parts of biocontrol bacteria, 5-10 parts of silicate bacteria and 1-5 parts of yield-increasing bacteria;
s2: observation characteristics: identifying the purity of flora in the raw materials, checking the production performance, checking whether the flora is polluted by other bacteria or not, observing the activity of the strains, selecting the flora with higher integrity, and putting the flora raw materials into a storage tube in sequence for freezing storage;
s3: and (3) purification: activating the flora in the cryopreservation tube in a slant, and purifying in a flat plate to obtain a strain slant;
s4: and (4) classification: classifying, counting and recording a plurality of flora slopes, preparing for synchronous culture, recording classified and counted information, and uploading recorded information in real time;
s5: fermentation culture: inoculating the purified flora slant into a seed culture medium bottle, placing the seed culture medium bottle into a shaking table for culture, simultaneously injecting air into the seed culture medium bottle by using an air compressor in the culture process, installing an air filter in the air circulation process, and sending sterile air into a seed tank;
s6: recording and uploading data on the seed tanks which are classified for culture, putting cultured flora into a main fermentation tank for secondary fermentation, putting culture medium raw materials and culture medium ingredients into a culture medium ingredient tank for mixing, respectively sending the mixed culture medium into a plurality of main fermentation tanks, and carrying out steam sterilization in the conveying process;
s7: the fungus crowd after will fermenting is put into the storage tank and is cooled off, puts into the agitator with multiple fungus crowd and mixes the stirring, adjusts pH value simultaneously, and the inside humidity state of real-time supervision when the stirring is carried dry air in to the agitator, dries it when the stirring is mixed, stops drying and stirring after reaching appointed numerical value with inside microbial inoculum humidity, takes out microbial composite inoculum and unifies the packing.
In the embodiment, in S3, the activation temperature is 36.5-37.2 ℃, and the activation time is 21-25 h.
In this example, in S3, the purification temperature is 36.8 to 37.5 ℃, and the activation time is 23 to 27 hours.
In this embodiment, in S5, the rotation speed of the shaking table is 180r/h, the set temperature of the shaking table is 36.8-37.2 ℃, and the working time of the shaking table is 16-20 h.
In this example, in the S6, the fermentation time is 12 to 16 hours, and the pH value is 7.0 to 7.3.
In this embodiment, in S7, the cooling time is 3 to 6 hours, and the pH is 7.0 to 7.5.
In the embodiment, the purity of the flora in the raw materials is identified, the production performance is checked, the flora is checked to see whether the flora is polluted by other bacteria or not, the activity of the strain is observed, the flora with higher integrity is selected, the flora raw materials are classified and sequentially placed into a storage tube for freezing storage, the flora in the freezing storage tube is activated in a slope and purified in a flat plate to obtain a strain slope, a plurality of flora slopes are classified, counted and recorded, synchronous culture is prepared, the information of the classification statistics is recorded, the recorded information is uploaded in real time, the purified flora slope is accessed into a seed culture medium bottle, the seed culture medium bottle is placed into a shaking table for culture, an air compressor is used for injecting air into the seed culture bottle during the culture process, an air filter is installed during the air circulation process, and sterile air is sent into a seed tank, data on the seed tank which is used for classifying and culturing are recorded and uploaded, cultured floras are placed into a main fermentation tank for secondary fermentation, culture medium raw materials and culture medium ingredients are placed into a culture medium ingredient tank for mixing, the mixed culture medium is respectively sent into a plurality of main fermentation tanks, steam sterilization is carried out in the conveying process, the fermented floras are placed into a storage tank for cooling, multiple floras are placed into a stirring barrel for mixing and stirring, the pH value is adjusted simultaneously, the internal humidity state is monitored in real time during stirring, dry air is conveyed into the stirring barrel, the dry air is dried while stirring and mixing, drying and stirring are stopped after the internal humidity reaches a specified value, and the microbial composite inoculant is taken out for uniform packaging.
Compared with the prior art, the invention has the technical progress that: the invention does not need to prepare a plurality of reagents in sequence, can be synchronously carried out side by side, has higher preparation efficiency, and has the advantages of mutual communication and comparison of data and higher processing efficiency.
Example two
Referring to fig. 1, a process technology of a compound microbial agent for apple planting comprises the following steps:
s1: preparing raw materials: 15 parts of photosynthetic bacteria, 10 parts of lactic acid bacteria, 15 parts of gram-positive actinomycetes, 25 parts of fermentation system filamentous bacteria, 10 parts of yeast bacteria, 10 parts of biocontrol bacteria, 10 parts of silicate bacteria and 5 parts of yield-increasing bacteria;
s2: observation characteristics: identifying the purity of flora in the raw materials, checking the production performance, checking whether the flora is polluted by other bacteria or not, observing the activity of the strains, selecting the flora with higher integrity, and putting the flora raw materials into a storage tube in sequence for freezing storage;
s3: and (3) purification: activating the flora in the cryopreservation tube in a slant, and purifying in a flat plate to obtain a strain slant;
s4: and (4) classification: classifying, counting and recording a plurality of flora slopes, preparing for synchronous culture, recording classified and counted information, and uploading recorded information in real time;
s5: fermentation culture: inoculating the purified flora slant into a seed culture medium bottle, placing the seed culture medium bottle into a shaking table for culture, simultaneously injecting air into the seed culture medium bottle by using an air compressor in the culture process, installing an air filter in the air circulation process, and sending sterile air into a seed tank;
s6: recording and uploading data on the seed tanks which are classified for culture, putting cultured flora into a main fermentation tank for secondary fermentation, putting culture medium raw materials and culture medium ingredients into a culture medium ingredient tank for mixing, respectively sending the mixed culture medium into a plurality of main fermentation tanks, and carrying out steam sterilization in the conveying process;
s7: the fungus crowd after will fermenting is put into the storage tank and is cooled off, puts into the agitator with multiple fungus crowd and mixes the stirring, adjusts pH value simultaneously, and the inside humidity state of real-time supervision when the stirring is carried dry air in to the agitator, dries it when the stirring is mixed, stops drying and stirring after reaching appointed numerical value with inside microbial inoculum humidity, takes out microbial composite inoculum and unifies the packing.
In this example, in S3, the activation temperature was 36.6 ℃ and the activation time was 22 hours.
In this example, in S3, the purification temperature was 36.8 ℃ and the activation time was 24 hours.
In this example, in S5, the rotation speed of the rocking platform is 140r/h, the set temperature of the rocking platform is 37.2 ℃, and the working time of the rocking platform is 20 h.
In this example, in S6, the fermentation time was 16 hours and the pH was 7.2.
In this example, in S7, the cooling time was 6 hours and the pH was 7.4.
EXAMPLE III
Referring to fig. 1, a process technology of a compound microbial agent for apple planting comprises the following steps:
s1: preparing raw materials: 20 parts of photosynthetic bacteria group, 20 parts of lactic acid bacteria group, 25 parts of gram-positive actinomycetes group, 10 parts of fermentation system filamentous bacteria group, 10 parts of yeast bacteria group, 5 parts of biocontrol bacteria, 5 parts of silicate bacteria and 5 parts of yield-increasing bacteria;
s2: observation characteristics: identifying the purity of flora in the raw materials, checking the production performance, checking whether the flora is polluted by other bacteria or not, observing the activity of the strains, selecting the flora with higher integrity, and putting the flora raw materials into a storage tube in sequence for freezing storage;
s3: and (3) purification: activating the flora in the cryopreservation tube in a slant, and purifying in a flat plate to obtain a strain slant;
s4: and (4) classification: classifying, counting and recording a plurality of flora slopes, preparing for synchronous culture, recording classified and counted information, and uploading recorded information in real time;
s5: fermentation culture: inoculating the purified flora slant into a seed culture medium bottle, placing the seed culture medium bottle into a shaking table for culture, simultaneously injecting air into the seed culture medium bottle by using an air compressor in the culture process, installing an air filter in the air circulation process, and sending sterile air into a seed tank;
s6: recording and uploading data on the seed tanks which are classified for culture, putting cultured flora into a main fermentation tank for secondary fermentation, putting culture medium raw materials and culture medium ingredients into a culture medium ingredient tank for mixing, respectively sending the mixed culture medium into a plurality of main fermentation tanks, and carrying out steam sterilization in the conveying process;
s7: the fungus crowd after will fermenting is put into the storage tank and is cooled off, puts into the agitator with multiple fungus crowd and mixes the stirring, adjusts pH value simultaneously, and the inside humidity state of real-time supervision when the stirring is carried dry air in to the agitator, dries it when the stirring is mixed, stops drying and stirring after reaching appointed numerical value with inside microbial inoculum humidity, takes out microbial composite inoculum and unifies the packing.
In this example, in S3, the activation temperature was 37 ℃ and the activation time was 25 hours.
In this example, in S3, the purification temperature was 37.5 ℃ and the activation time was 27 hours.
In this example, in S5, the rotation speed of the rocking bed was 180r/h, the set temperature of the rocking bed was 37.2 ℃, and the working time of the rocking bed was 20 h.
In this example, in S6, the fermentation time was 16 hours and the pH was 7.3.
In this example, in S7, the cooling time was 3 hours and the pH was 7.5.
Claims (6)
1. A process technology of a compound microbial agent for apple planting is characterized by comprising the following steps:
s1: preparing raw materials: 15-30 parts of photosynthetic bacteria, 10-20 parts of lactic acid bacteria, 15-25 parts of gram-positive actinomycetes, 10-25 parts of fermentation system filamentous bacteria, 10-25 parts of yeast bacteria, 5-10 parts of biocontrol bacteria, 5-10 parts of silicate bacteria and 1-5 parts of yield-increasing bacteria;
s2: observation characteristics: identifying the purity of flora in the raw materials, checking the production performance, checking whether the flora is polluted by other bacteria or not, observing the activity of the strains, selecting the flora with higher integrity, and putting the flora raw materials into a storage tube in sequence for freezing storage;
s3: and (3) purification: activating the flora in the cryopreservation tube in a slant, and purifying in a flat plate to obtain a strain slant;
s4: and (4) classification: classifying, counting and recording a plurality of flora slopes, preparing for synchronous culture, recording classified and counted information, and uploading recorded information in real time;
s5: fermentation culture: inoculating the purified flora slant into a seed culture medium bottle, placing the seed culture medium bottle into a shaking table for culture, simultaneously injecting air into the seed culture medium bottle by using an air compressor in the culture process, installing an air filter in the air circulation process, and sending sterile air into a seed tank;
s6: recording and uploading data on the seed tanks which are classified for culture, putting cultured flora into a main fermentation tank for secondary fermentation, putting culture medium raw materials and culture medium ingredients into a culture medium ingredient tank for mixing, respectively sending the mixed culture medium into a plurality of main fermentation tanks, and carrying out steam sterilization in the conveying process;
s7: the fungus crowd after will fermenting is put into the storage tank and is cooled off, puts into the agitator with multiple fungus crowd and mixes the stirring, adjusts pH value simultaneously, and the inside humidity state of real-time supervision when the stirring is carried dry air in to the agitator, dries it when the stirring is mixed, stops drying and stirring after reaching appointed numerical value with inside microbial inoculum humidity, takes out microbial composite inoculum and unifies the packing.
2. The process technology of the compound microbial inoculant for apple cultivation according to claim 1, wherein in the S3, the activation temperature is 36.5-37.2 ℃, and the activation time is 21-25 h.
3. The process technology of the compound microbial inoculant for apple cultivation as claimed in claim 1, wherein in the S3, the purification temperature is 36.8-37.5 ℃, and the activation time is 23-27 h.
4. The process of claim 1, wherein in S5, the rotation speed of the rocking bed is 130-180r/h, the temperature of the rocking bed is 36.8-37.2 ℃, and the working time of the rocking bed is 16-20 h.
5. The process technology of the compound microbial inoculant for apple cultivation as claimed in claim 1, wherein in the S6, the fermentation time is 12-16h and the pH value is 7.0-7.3.
6. The process technology of the compound microbial inoculant for apple cultivation as claimed in claim 1, wherein in the S7, the cooling time is 3-6h, and the pH value is 7.0-7.5.
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| CN110184188A (en) * | 2019-06-19 | 2019-08-30 | 安徽万士生物制药有限公司 | A kind of production technology of complex microbial inoculum |
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2021
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| CN102838390A (en) * | 2012-08-31 | 2012-12-26 | 成都市家家美食品有限公司 | Ecological organic fertilizer |
| CN107118045A (en) * | 2017-07-11 | 2017-09-01 | 烟台三合生物科技有限公司 | A kind of technology for the complex micro organism fungicide that apple cultivation is used |
| CN108239602A (en) * | 2017-12-04 | 2018-07-03 | 连云港巨森农业科技发展有限公司 | A kind of production technology of complex microbial inoculum |
| CN110184188A (en) * | 2019-06-19 | 2019-08-30 | 安徽万士生物制药有限公司 | A kind of production technology of complex microbial inoculum |
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