CN110184188A - A kind of production technology of complex microbial inoculum - Google Patents
A kind of production technology of complex microbial inoculum Download PDFInfo
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- CN110184188A CN110184188A CN201910531634.2A CN201910531634A CN110184188A CN 110184188 A CN110184188 A CN 110184188A CN 201910531634 A CN201910531634 A CN 201910531634A CN 110184188 A CN110184188 A CN 110184188A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention discloses a kind of production technologies of complex microbial inoculum, are related to microorganism complex technical field.The present invention is the following steps are included: S001, production is identified with strain；It sexually revises or to function has occurred by the purifying or rejuvenation of the strain progress strain of living contaminants；S002 carries out lab pilot plant to strain before strain is mass produced, and determines the suitable growth temperature of strain and grows the suitable component and ratio of PH and fermentation medium；And determine the suitable incubation time of strain, draw the growth curve of strain.The present invention by strain is identified before manufacture and growing environment test；Then slant activation and Spawn incubation are carried out, seed liquor and shaking flask strain are respectively prepared, complex microbial inoculum is finally made by seeding tank fermentation and ferment tank respectively, this complex microbial inoculum manufacturing process simply, low manufacture cost；The meat of domestic animal is effectively guaranteed in the quality for improving microbial bacterial agent.
Description
Technical field
The invention belongs to microorganism complex technical fields, more particularly to a kind of production work of complex microbial inoculum
Skill.
Background technique
As the outgoing fluidity of labor increases, individual domestic animal decentralized household is fewer and fewer, and the large aquaculture family of idiotype
It is more and more, in the breeding process of domestic animal, realize the supplement of domestic animal microorganism to domestic animal with feed for nursing or other modes
Growth it is most important, this is directly concerning the health status of domestic animal and the meat of domestic animal.
And existing complex microbial inoculum manufacturing process is cumbersome, and the qualities such as activity of complex microbial inoculum can not protect
Card, can not provide booster action for the good constitution of domestic animal；Therefore a kind of production work for complex microbial inoculum that requires study
Skill carrys out the technical process of simplifying microbial composite bacteria agent, and guarantees complex microbial inoculum quality.
Summary of the invention
The purpose of the present invention is to provide a kind of production technology of complex microbial inoculum, by before manufacture to strain into
Row identification and growing environment test；Then it carries out slant activation and seed liquor and shaking flask strain is respectively prepared in Spawn incubation, finally
Respectively by seeding tank fermentation and ferment tank, complex microbial inoculum is made；Solves existing complex microbial inoculum system
It is cumbersome to make process, and the problem of complex microbial inoculum quality not can guarantee.
In order to solve the above technical problems, the present invention is achieved by the following technical solutions:
The present invention is a kind of production technology of complex microbial inoculum, comprising the following steps:
S001 identifies production with strain；It sexually revises or to function has occurred by the strain progress bacterium of living contaminants
The purifying or rejuvenation of kind；
S002 carries out lab pilot plant to strain, determines that strain is suitably grown before strain is mass produced
The suitable component and ratio of temperature and growth PH and fermentation medium；And determine the suitable incubation time of strain, draw strain
Growth curve；
Strain in freezing pipe is activated in inclined-plane, and is purified in plate by S003, finally obtains inclined-plane
Strain or strain inclined plane；
S004, takes the strain of a ring after purification, and access is placed in shaking table and carries out equipped in the triangular flask of seed culture medium
Culture, is made seed liquor；
S005 takes a certain amount of seed liquor, and access is equipped in the triangular flask of fermentation medium, and this triangular flask is placed in shaking table
Shaking flask strain is made in middle culture；
S006 sterilizes to strain fermentation equipment using sterile steam, and a certain amount of seed is added into seeding tank
Tank culture medium；PH value detection, sterilizing and cooling are carried out to seeding tank；And access shaking flask strain in seeding tank, it is empty to be passed through sterilizing
Gas and defoaming agent ferment, and obtain seeding tank fermentation liquid；
S007 inserts fermentation tank culture medium into fermentor, and carries out pH value detection, sterilizing and cooling to fermentor；To
Seeding tank fermentation liquid is accessed in fermentor, and is passed through sterile air and defoaming agent, obtains complex microbial inoculum.
Further, the strain idenfication in the S001 include strain Purity, strain production performance inspection,
The activity of strain.
Further, the inclined-plane culture based component in the S003 are as follows: glucose 0.1%, beef extract 1%, peptone
1%, Nacl0.5%, agar 2-2.5%；The pH value of slant medium is 7-7.2.
Further, the medium component in the triangular flask in the S004 and S005 are as follows: glucose 2.5%, urea
0.5%, magnesium sulfate 0.04%, dipotassium hydrogen phosphate 0.1%, corn pulp 2.5-3.5%, ferrous sulfate 2ppm, manganese sulfate 2PPm；Three
Culture medium pH value in the bottle of angle is 7.
Further, the inoculum concentration of the seed liquor in the access triangular flask in the S005 is the practical culture medium dress of triangular flask
The 4-5% of amount.
Further, the shaking flask strain in the S006 accounts for the 1-6% of the practical charge of seeding tank.
Further, the seed tank culture based component in the S006 are as follows: hydrolysis sugar 2.6%, corn pulp 3%, sulphur
Sour magnesium 0.05%, dipotassium hydrogen phosphate 0.2%, urea 0.5ppm, ferro element 2ppm, manganese element 2ppm；The seed tank culture base
PH value be 6.9.
Further, the ingredient of the fermentation tank culture medium in the S007 are as follows: hydrolysis sugar 13%, cane molasses
0.15%, urea 0.6%, dipotassium hydrogen phosphate 0.17%, urea 0.05ppm, ferrous sulfate 2ppm, manganese sulfate 2ppm, water 80-
90%；The pH value of the fermentation tank culture medium is 7.
The invention has the following advantages:
The present invention by strain is identified before manufacture and growing environment test；Then slant activation and strain are carried out
Seed liquor and shaking flask strain is respectively prepared in culture, and it is multiple that microorganism finally is made by seeding tank fermentation and ferment tank respectively
Combined bacteria agent, this complex microbial inoculum manufacturing process is simple, low manufacture cost；The quality of microbial bacterial agent is improved, effectively
It ensure that the meat of domestic animal.
Certainly, it implements any of the products of the present invention and does not necessarily require achieving all the advantages described above at the same time.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, will be described below to embodiment required
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 is a kind of production technological process of complex microbial inoculum of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, the present invention is a kind of production technology of complex microbial inoculum, comprising the following steps:
S001 identifies production with strain；It sexually revises or to function has occurred by the strain progress bacterium of living contaminants
The purifying or rejuvenation of kind；
S002 carries out lab pilot plant to strain, determines the most suitable growth of strain before strain is mass produced
The most suitable ingredient and ratio of temperature and growth PH and fermentation medium；And determine the suitable incubation time of strain, draw strain
Growth curve；We generally choose strain of the thallus of logarithmic growth phase as production fermentation；S001 and S002 belongs to micro-
Prepare before the production of biological compound fungi agent；
S003 activates the strain in freezing pipe in inclined-plane, and the temperature of activation is 37 °, and the time is for 24 hours；And
It is purified in plate, the temperature and time of purifying is respectively 37 ° and for 24 hours, finally obtains slant strains or strain inclined plane；
S004, takes the strain of a ring after purification, and access loading amount is juxtaposition in the 250ml triangular flask of 20ml seed culture medium
It is cultivated in 180r/min shaking table, the cultivation temperature and time in shaking table are respectively 37 ° and 18h, and seed liquor is made；
S005, takes the seed liquor of 1ml, and access is equipped in the 250ml triangular flask of 20ml fermentation medium, and by this triangular flask
It is placed in 180r/min shaking table and cultivates, the cultivation temperature and time in shaking table are respectively 37 ° and for 24 hours, and shaking flask strain is made；
S006 sterilizes to strain fermentation equipment using sterile steam, and the temperature to the sterilizing of strain fermentation equipment is 121
DEG C~125 DEG C, pressure is 0.103MPa~0168MPa, and the time is 0.5h~1.0h, and a certain amount of kind is added into seeding tank
Sub- tank culture medium, the 55-65% of seed tank culture fiduciary point seed tank volume；PH value detection is carried out to seeding tank, and controls seed
The pH value of tank is passed through sterile air, seeding tank temperature is made to drop to 20 DEG C~30 DEG C in 7, sterilizing and cooling；And shaking flask strain is connect
Enter in seeding tank, is passed through sterile air and defoaming agent ferments, obtain seeding tank fermentation liquid, pass through microexamination seeding tank
The form density and gemma rate of fermentation liquid；
S007, inserts fermentation tank culture medium into fermentor, and the 50%~60% of fermentation tank culture fiduciary point fermenter volume,
And pH value detection, sterilizing and cooling are carried out to fermentor, pH value guarantees that the temperature of fermentor sterilizing is 121 DEG C~125 DEG C 7,
Pressure is 0.103MPa~0168MPa, and the time is 0.5h~1.0h, and temperature is maintained at 25 DEG C~30 DEG C；It is accessed into fermentor
Seeding tank fermentation liquid, and it is passed through sterile air and defoaming agent, seeding tank fermentation liquid is placed into 25- at a temperature of 25 DEG C~30 DEG C
35h obtains complex microbial inoculum, carries out storage tank, and 10~20 DEG C of liquid microbial inoculum solid content to complex microbial inoculum.
Wherein, the strain idenfication in S001 includes strain Purity, the production performance inspection of strain, the work of strain
Property, whether there is or not living contaminants, whether correlated performance degenerates.
Wherein, the inclined-plane culture based component in S003 are as follows: glucose 0.1%, beef extract 1%, peptone 1%,
Nacl0.5%, agar 2-2.5%；The pH value of slant medium is 7-7.2.
Wherein, the medium component in the triangular flask in S004 and S005 are as follows: glucose 2.5%, urea 0.5%, sulfuric acid
Magnesium 0.04%, dipotassium hydrogen phosphate 0.1%, corn pulp 2.5-3.5%, ferrous sulfate 2ppm, manganese sulfate 2PPm；Training in triangular flask
The pH value for supporting base is 7.
Wherein, the inoculum concentration of the seed liquor in the access triangular flask in S005 is the 4- of the practical culture medium loading amount of triangular flask
5%.
Wherein, the shaking flask strain in S006 accounts for the 1-6% of the practical charge of seeding tank.
Wherein, the seed tank culture based component in S006 are as follows: hydrolysis sugar 2.6%, corn pulp 3%, magnesium sulfate 0.05%, phosphorus
Sour hydrogen dipotassium 0.2%, urea 0.5ppm, ferro element 2ppm, manganese element 2ppm；The pH value of seed tank culture base is 6.9.
Wherein, the ingredient of the fermentation tank culture medium in S007 are as follows: hydrolysis sugar 13%, cane molasses 0.15%, urea
0.6%, dipotassium hydrogen phosphate 0.17%, urea 0.05ppm, ferrous sulfate 2ppm, manganese sulfate 2ppm, water 80-90%；Fermentor training
The pH value for supporting base is 7.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (8)
1. a kind of production technology of complex microbial inoculum, which comprises the following steps:
S001 identifies production with strain；It sexually revises or to function has occurred by the strain progress strain of living contaminants
Purifying or rejuvenation；
S002 carries out lab pilot plant to strain, determines the suitable growth temperature of strain before strain is mass produced
With the suitable component and ratio of growth PH and fermentation medium；And determine the suitable incubation time of strain, draw the life of strain
Long curve；
Strain in freezing pipe is activated in inclined-plane, and is purified in plate by S003, finally obtains slant strains
Or strain inclined plane；
S004, takes the strain of a ring after purification, and access is placed in shaking table and is trained equipped in the triangular flask of seed culture medium
It supports, seed liquor is made；
S005 takes a certain amount of seed liquor, and access is equipped in the triangular flask of fermentation medium, and this triangular flask is placed in shaking table and is trained
It supports, shaking flask strain is made；
S006 sterilizes to strain fermentation equipment using sterile steam, and a certain amount of seeding tank training is added into seeding tank
Support base；PH value detection, sterilizing and cooling are carried out to seeding tank；And will shaking flask strain access seeding tank in, be passed through sterile air and
Defoaming agent ferments, and obtains seeding tank fermentation liquid；
S007 inserts fermentation tank culture medium into fermentor, and carries out pH value detection, sterilizing and cooling to fermentor；To fermentation
Seeding tank fermentation liquid is accessed in tank, and is passed through sterile air and defoaming agent, obtains complex microbial inoculum.
2. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S001
The strain idenfication includes the activity of strain Purity, the production performance inspection of strain, strain.
3. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S003
Inclined-plane culture based component are as follows: glucose 0.1%, beef extract 1%, peptone 1%, Nacl0.5%, agar 2-2.5%；Inclined-plane training
The pH value for supporting base is 7-7.2.
4. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that the S004 and
The medium component in triangular flask in S005 are as follows: glucose 2.5%, urea 0.5%, magnesium sulfate 0.04%, dipotassium hydrogen phosphate
0.1%, corn pulp 2.5-3.5%, ferrous sulfate 2ppm, manganese sulfate 2PPm；Culture medium pH value in triangular flask is 7.
5. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S005
The inoculum concentration for accessing the seed liquor in triangular flask is the 4-5% of the practical culture medium loading amount of triangular flask.
6. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S006
The shaking flask strain accounts for the 1-6% of the practical charge of seeding tank.
7. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S006
The seed tank culture based component are as follows: hydrolysis sugar 2.6%, corn pulp 3%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.2%, urine
Plain 0.5ppm, ferro element 2ppm, manganese element 2ppm；The pH value of the seed tank culture base is 6.9.
8. a kind of production technology of complex microbial inoculum according to claim 1, which is characterized in that in the S007
The ingredient of the fermentation tank culture medium are as follows: hydrolysis sugar 13%, cane molasses 0.15%, urea 0.6%, dipotassium hydrogen phosphate
0.17%, urea 0.05ppm, ferrous sulfate 2ppm, manganese sulfate 2ppm, water 80-90%；The pH value of the fermentation tank culture medium is
7。
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| CN201910531634.2A CN110184188A (en) | 2019-06-19 | 2019-06-19 | A kind of production technology of complex microbial inoculum |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110922975A (en) * | 2019-12-23 | 2020-03-27 | 南京朴厚生态科技有限公司 | Preparation method and application of microbial straw degradation microbial inoculum |
| CN114149929A (en) * | 2021-12-15 | 2022-03-08 | 乌拉特前旗尚鸿林果科技有限公司 | Process technology of compound microbial agent for apple planting |
| CN115340950A (en) * | 2022-09-09 | 2022-11-15 | 漯河市田润宝生物科技有限公司 | Nutritional growth-promoting microbial fermentation process |
-
2019
- 2019-06-19 CN CN201910531634.2A patent/CN110184188A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110922975A (en) * | 2019-12-23 | 2020-03-27 | 南京朴厚生态科技有限公司 | Preparation method and application of microbial straw degradation microbial inoculum |
| CN114149929A (en) * | 2021-12-15 | 2022-03-08 | 乌拉特前旗尚鸿林果科技有限公司 | Process technology of compound microbial agent for apple planting |
| CN115340950A (en) * | 2022-09-09 | 2022-11-15 | 漯河市田润宝生物科技有限公司 | Nutritional growth-promoting microbial fermentation process |
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Application publication date: 20190830 |