CN114134131A - 一种深海g型（g-type）溶菌酶的应用 - Google Patents

一种深海g型（g-type）溶菌酶的应用 Download PDF

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CN114134131A
CN114134131A CN202111430200.7A CN202111430200A CN114134131A CN 114134131 A CN114134131 A CN 114134131A CN 202111430200 A CN202111430200 A CN 202111430200A CN 114134131 A CN114134131 A CN 114134131A
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孙黎
罗晶昶
张健
管晓璐
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Abstract

本发明涉及分子生物学领域，具体的说是一种深海g型(g‑type)溶菌酶的应用。所述深海g型溶菌酶在用于制备杀菌制剂的应用。所述深海g型溶菌酶为在大肠杆菌中表达获得的重组蛋白。本发明的重组g型溶菌酶用于杀菌。

Description

一种深海g型（g-type）溶菌酶的应用
技术领域
本发明涉及分子生物学领域，具体的说是一种深海g型(g-type)溶菌酶的应用。
背景技术
溶菌酶(muramidase，EC 3.2.1.17)是先天免疫系统的关键分子之一。根据溶菌酶来源、氨基酸序列、化学性质和生物活性的差异，可以将溶菌酶分为六种类型，其中一种为g型(goose-type，g-type)。
肽聚糖是细菌细胞壁的一个重要组成部分，溶菌酶能催化其中N-乙酰壁酸(MurNAc)的C-1和N-乙酰氨基葡萄糖(GlcNAc)的C-4之间的β-1,4- 糖苷键的分裂。因此，溶菌酶被广泛应用，并作为一种“武器”用以对抗细菌感染。与革兰氏阴性菌相比，革兰氏阳性菌的肽聚糖细胞壁要厚得多，并且暴露在细胞外环境中，溶菌酶因此通常可以更有效地杀死革兰氏阳性菌。
在近些年的报道中，发现溶菌酶中部分类型具有除溶壁酶活性之外的其他功能，比如异肽酶、几丁质酶和非酶活的抗菌活性。另外，一些溶菌酶还用于炎症治疗中，比如用于缓解炎症性肠病的免疫反应，和通过调节血清补体活化来影响炎症。
由此可见，尽管有许多g型溶菌酶被发现报道，并且在杀菌实验中大都可以表现出对细菌生长的抑制，但不同溶菌酶对不同细菌的杀菌效果并不一致，尤其是深海来源的g型溶菌酶更鲜有报道，进而需要对其进行深入的研究。
发明内容
本发明目的在于提供一种深海g型溶菌酶的应用。
为实现上述目的，本发明采用的技术方案为：
一种深海g型溶菌酶的应用，所述深海g型溶菌酶在用于制备杀菌制剂的应用。
所述深海g型溶菌酶为源于深海马努斯盆地热液喷口阿尔文虾的转录组。
所述深海g型溶菌酶如SEQ ID No.1所示的氨基酸序列。
所述深海g型溶菌酶为SEQ ID No.1所示的氨基酸序列在大肠杆菌中表达获得的重组蛋白。
所述细菌为大肠杆菌(Escherichia coli)、迟缓爱德华氏菌(Edwardsiellatarda)或荧光假单胞菌(Pseudomonas fluorescens)。
本发明具有如下优点：
本发明的g型溶菌酶来自深海，即源于深海马努斯盆地热液喷口阿尔文虾的转录组，其N端无信号肽，中间部分有结合催化区域；所得源深海g型溶菌酶能够特异性杀死大肠杆菌、迟缓爱德华氏菌和荧光假单胞菌。
附图说明
图1为本发明实施例提供的重组g型溶菌酶蛋白对细菌的杀伤作用。
具体实施方式
下面结合实施例对本发明作进一步说明。实施例旨在对本发明进行举例描述，而非以任何形式对本发明进行限制。
实施例1
本发明中的g型溶菌酶(命名为LysG1)来源于深海马努斯盆地热液喷口阿尔文虾的转录组，在NCBI数据库中记载GenBank登录号为： SRR4342052。g型溶菌酶序列为表SEQID No.1中的氨基酸序列。
序列表SEQ ID No.1为：
MGYGNLRNVSTTGASNTTAQQDGPTSLCGVAASHKLAKTDLQRMKGYKQKISSVGTRHDIDSAIIAGIISRESRAGNALEGGYGDHGKAFGLMQIDQGASGVQPKGAWDSEEHLYQATGMLIDFIKTMGNKSAFQGLSKEKKLK AGIAAYNMGPGNVH SYDTVDKHTTGGDYSNDVIARAQYYKNNGF
(a)序列特征：
·长度：188
·类型：氨基酸序列
·链型：单链
·拓扑结构：线性
(b)分子类型：蛋白质
(c)信号肽序列：无
(d)假设：否
(e)反义：否
(f)最初来源：深海阿尔文虾
所述LysG1分子量为20.0kDa，N端无信号肽，中间部分有结合催化区域(下划线表示)。
实施例2
LysG1和硫氧还蛋白(Trx)的重组蛋白的制备
1)表达LysG1重组蛋白的质粒pEt-LysG1的构建：
根据上述获得LysG1的氨基酸序列，由上海生工生物工程股份有限公司合成并连接至表达载体pET-28a，构建重组质粒pEt-LysG1。重组质粒经 DNA测序表明pEt-LysG1含有编码LysG1序列的基因。
2)重组LysG1蛋白(rLysG1)的诱导表达和纯化
将上述的质粒pEt-LysG1用常规方法转化大肠杆菌BL21(DE3)(购自于“北京擎科生物科技有限公司”，北京)，在含有卡那霉素(50μg/ml) 的LB固体培养基上培养18小时,挑取转化子，将其命名为BL21/pEt-LysG1。将BL21/pEt-LysG1于含有卡那霉素(50μg/ml)的LB液体培养基中过夜培养；取5ml过夜后的培养液,加入500ml新鲜的含有卡那霉素(50μg/ml)的LB液体培养基中，于37℃下转速180rpm摇动培养至OD600为0.6，加入终浓度为0.04mM的IPTG，16℃继续以转速100rpm摇动培养15h，而后以 6000g，4℃离心10min，收集菌液。加入30ml裂解液，至于液氮中冷冻20min， 4℃融化后超声破碎60min至细菌悬液变澄清。将菌液以10000g，4℃离心 30min，回收上清。将上清中的蛋白用Ni-NTA蛋白纯化树脂(购自于“QIAGEN 公司”，德国)回收纯化，将纯化的蛋白悬浮于PBS缓冲液，命名为rLysG1。
所述裂解液为终浓度的9.5mM NaH2PO4、40mM Na2HPO4、0.5M NaCl 和10mM咪唑,调节pH 8.0。
所述PBS组成成分按重量百分比计：0.8％NaCl,0.02％KCl,0.358％Na2HPO4.12H2O,0.024％NaH2PO4，余量为蒸馏水。
所述LB固体培养基成分：胰蛋白胨10g/L，酵母提取物5g/L，氯化钠10g/L，琼脂糖15g/L
所述LB液体培养基成分：胰蛋白胨10g/L，酵母提取物5g/L，氯化钠10g/L。
3)重组Trx蛋白(rTrx)的诱导表达和纯化
将pET32a(购自于“Novagen公司”，美国)用上述方法转化大肠杆菌BL21(DE3)，在含有氨苄青霉素(100μg/ml)的LB固体培养基上培养 18小时,挑取转化子，将其命名为BL21/pET32a。将BL21/pET32a于含有氨苄青霉素(100μg/ml)的LB液体培养基中过夜培养；取5ml过夜后的培养液,加入500ml新鲜的含有安卡青霉素(100μg/ml)的LB液体培养基中，于37℃下转速180rpm摇动培养至OD600为0.6，加入终浓度为0.1mM的IPTG， 16℃以转速100rpm摇动培养15h，而后以6000g，4℃离心10min，收集菌液。加入30ml裂解液，至于液氮冷冻20min，4℃融化后超声破碎60min至细菌悬液变澄清。将菌液以10000g，4℃离心30min，回收上清。将上清中的蛋白用Ni-NTA蛋白纯化树脂(购自于“QIAGEN公司”，德国)回收纯化，将纯化的蛋白悬浮于PBS缓冲液，命名为rTrx。
实施例3
rLysG1的杀菌应用
步骤1)细菌悬液制备
在LB液体培养基中培养大肠杆菌(Escherichia coli)DH5α、迟缓爱德华氏菌(Edwardsiella tarda)TX1和荧光假单胞菌(Pseudomonas fluorescens)TSS1至OD600为0.8,然后离心(5000g，4℃，10min)，收集菌体，将其悬浮于PBS中至终浓度为103cfu/ml，即为细菌悬液。
所述大肠杆菌DH5α购买于北京擎科生物科技有限公司。
所述迟缓爱德华氏菌(Edwardsiella tarda)TX1保存于中国微生物菌种保藏管理委员会普通微生物中心CGMCC，保藏编号为：CGMCC No.2330，保藏日期2008.1.9，分类命名为迟缓爱德华氏菌(Edwardsiella tarda)。
所述荧光假单胞菌(Pseudomonas fluorescens)TSS1保存于中国微生物菌种保藏管理委员会普通微生物中心CGMCC，保藏编号为：CGMCC No.2329，保藏日期2008.1.9，分类命名为荧光假单胞菌(Pseudomonas fluorescens)。
步骤2)rLysG1杀菌检测
取200μl上述步骤1)的大肠杆菌、迟缓爱德华氏菌和荧光假单胞菌，分别置于1.5ml离心管中，向细菌悬液中加入rLysG1(200μg/ml)、rTrx (200μg/ml)或PBS(对照)，进行杀菌能力的检测。实验组和对照组在室温孵育1h后，涂LB固体培养基平板。培养15小时后进行菌落计数。发现经rLysG1处理的迟缓爱德华氏菌、大肠杆菌和荧光假单胞菌的菌落数均显著减少，其中大肠杆菌和荧光假单胞菌对rLysG1尤为敏感，存活率低于 10％，而对照蛋白rTrx对三种细菌的存活都没有影响(图1)。实验数据为三次平行实验结果。**p<0.01。这些结果表明，本发明的rLysG1对迟缓爱德华氏菌、大肠杆菌和荧光假单胞菌具有显著的杀伤能力。
序列表
<110> 中国科学院海洋研究所
<120> 一种深海g型（g-type）溶菌酶的应用
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Asp Thr Val Asp Lys His Thr Thr Gly Gly Asp Tyr Ser Asn Asp Val
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Claims (5)

1.一种深海g型溶菌酶的应用，其特征在于：所述深海g型溶菌酶在用于制备杀菌制剂的应用。
2.按权利要求1所述的应用，其特征在于：所述深海g型溶菌酶为源于深海马努斯盆地热液喷口阿尔文虾的转录组。
3.按权利要求1所述的应用，其特征在于：所述深海g型溶菌酶如SEQ ID No.1所示的氨基酸序列。
4.按权利要求1-3任意一项所述的应用，其特征在于：所述深海g型溶菌酶为SEQ IDNo.1所示的氨基酸序列在大肠杆菌中表达获得的重组蛋白。
5.按权利要求1所述的应用，其特征在于：所述细菌为大肠杆菌(Escherichia coli)、迟缓爱德华氏菌(Edwardsiella tarda)或荧光假单胞菌(Pseudomonas fluorescens)。
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CN109628477A (zh) * 2018-12-25 2019-04-16 国家海洋局第三海洋研究所 一种超深渊来源溶菌酶及其制备方法

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