CN114127307B - 细胞图像解析方法和细胞图像解析装置 - Google Patents

细胞图像解析方法和细胞图像解析装置 Download PDF

Info

Publication number
CN114127307B
CN114127307B CN201980098569.7A CN201980098569A CN114127307B CN 114127307 B CN114127307 B CN 114127307B CN 201980098569 A CN201980098569 A CN 201980098569A CN 114127307 B CN114127307 B CN 114127307B
Authority
CN
China
Prior art keywords
image
colony
cell
area
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980098569.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN114127307A (zh
Inventor
高桥涉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Publication of CN114127307A publication Critical patent/CN114127307A/zh
Application granted granted Critical
Publication of CN114127307B publication Critical patent/CN114127307B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/20Image preprocessing
    • G06V10/22Image preprocessing by selection of a specific region containing or referencing a pattern; Locating or processing of specific regions to guide the detection or recognition
    • G06V10/235Image preprocessing by selection of a specific region containing or referencing a pattern; Locating or processing of specific regions to guide the detection or recognition based on user input or interaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/34Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/40Extraction of image or video features
    • G06V10/46Descriptors for shape, contour or point-related descriptors, e.g. scale invariant feature transform [SIFT] or bags of words [BoW]; Salient regional features
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/70Arrangements for image or video recognition or understanding using pattern recognition or machine learning
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00Arrangements for image or video recognition or understanding
    • G06V10/70Arrangements for image or video recognition or understanding using pattern recognition or machine learning
    • G06V10/77Processing image or video features in feature spaces; using data integration or data reduction, e.g. principal component analysis [PCA] or independent component analysis [ICA] or self-organising maps [SOM]; Blind source separation
    • G06V10/774Generating sets of training patterns; Bootstrap methods, e.g. bagging or boosting
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00Scenes; Scene-specific elements
    • G06V20/60Type of objects
    • G06V20/69Microscopic objects, e.g. biological cells or cellular parts
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06VIMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00Scenes; Scene-specific elements
    • G06V20/60Type of objects
    • G06V20/69Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695Preprocessing, e.g. image segmentation

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Multimedia (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medical Informatics (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Computation (AREA)
  • Computing Systems (AREA)
  • Software Systems (AREA)
  • Databases & Information Systems (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Analysis (AREA)
CN201980098569.7A 2019-07-18 2019-07-18 细胞图像解析方法和细胞图像解析装置 Active CN114127307B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/028268 WO2021009906A1 (ja) 2019-07-18 2019-07-18 細胞画像解析方法および細胞画像解析装置

Publications (2)

Publication Number Publication Date
CN114127307A CN114127307A (zh) 2022-03-01
CN114127307B true CN114127307B (zh) 2025-04-04

Family

ID=74210370

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980098569.7A Active CN114127307B (zh) 2019-07-18 2019-07-18 细胞图像解析方法和细胞图像解析装置

Country Status (4)

Country Link
US (1) US12394170B2 (https=)
JP (1) JP7342950B2 (https=)
CN (1) CN114127307B (https=)
WO (1) WO2021009906A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113470037A (zh) 2020-03-31 2021-10-01 阿里巴巴集团控股有限公司 数据处理方法、装置及系统
CN113469180A (zh) * 2020-03-31 2021-10-01 阿里巴巴集团控股有限公司 医学图像的处理方法和系统、数据处理方法
WO2023008428A1 (ja) * 2021-07-29 2023-02-02 株式会社島津製作所 細胞画像解析方法
JP7769966B2 (ja) * 2021-08-30 2025-11-14 株式会社サイト-ファクト 細胞画像解析システム、細胞画像解析装置、および、細胞画像解析方法
CN115700821B (zh) * 2022-11-24 2023-06-06 广东美赛尔细胞生物科技有限公司 一种基于图像处理的细胞识别方法及系统
EP4629183A4 (en) * 2022-12-30 2026-01-21 Bgi Shenzhen METHOD AND SYSTEM FOR RECORDING CELL IMAGES, ELECTRONIC DEVICE AND STORAGE MEDIA
JP2024101940A (ja) * 2023-01-18 2024-07-30 キヤノン株式会社 細胞画像解析方法、プログラム、推論モデルの製造方法および細胞画像解析装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011229409A (ja) * 2010-04-23 2011-11-17 Nagoya Univ 細胞評価装置、インキュベータ、細胞評価方法、細胞評価プログラムおよび細胞の培養方法
WO2018105432A1 (ja) * 2016-12-06 2018-06-14 富士フイルム株式会社 細胞画像評価装置および細胞画像評価制御プログラム

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5181385B2 (ja) 2007-08-16 2013-04-10 国立大学法人名古屋大学 細胞の品質を予測する予測モデルの構築法、予測モデルの構築用ブログラム、該プログラムを記録した記録媒体、予測モデルの構築用装置
JP4968595B2 (ja) 2008-07-23 2012-07-04 株式会社ニコン 細胞の状態判別手法及び細胞観察の画像処理装置
JP2013201909A (ja) * 2012-03-27 2013-10-07 National Institute Of Advanced Industrial Science & Technology 細胞画像判定装置、方法、並びにプログラム
JP2014235494A (ja) * 2013-05-31 2014-12-15 富士ゼロックス株式会社 画像処理装置及びプログラム
JP6097951B2 (ja) * 2013-08-22 2017-03-22 富士フイルム株式会社 幹細胞分化判定装置および方法並びにプログラム
US9619881B2 (en) * 2013-09-26 2017-04-11 Cellogy, Inc. Method and system for characterizing cell populations
JP6595156B2 (ja) * 2014-03-04 2019-10-23 富士フイルム株式会社 細胞画像取得装置および方法並びにプログラム
JP6130801B2 (ja) * 2014-03-17 2017-05-17 富士フイルム株式会社 細胞領域表示制御装置および方法並びにプログラム
JP6543954B2 (ja) * 2015-02-24 2019-07-17 シンフォニアテクノロジー株式会社 除去領域設定方法、除去領域設定装置およびプログラム
JP2017068349A (ja) * 2015-09-28 2017-04-06 株式会社Screenホールディングス 画像処理方法および制御プログラム
EP3677943A4 (en) * 2017-08-29 2020-09-16 FUJIFILM Corporation DEVICE, METHOD AND PROGRAM FOR IMAGE CONTROL
EP3721373B1 (en) * 2017-12-07 2025-03-05 Ventana Medical Systems, Inc. Deep-learning systems and methods for joint cell and region classification in biological images
CN111837157B (zh) * 2018-03-08 2024-08-23 株式会社岛津制作所 细胞图像解析方法、细胞图像解析装置、及学习模型创建方法
EP3766000A2 (en) * 2018-03-16 2021-01-20 The United States of America as represented by the Secretary of the Department of Health and Human Services Using machine learning and/or neural networks to validate stem cells and their derivatives for use in cell therapy, drug discovery, and diagnostics
JP6996633B2 (ja) * 2018-08-06 2022-01-17 株式会社島津製作所 教師ラベル画像修正方法、学習済みモデルの作成方法および画像解析装置
JP7049974B2 (ja) * 2018-10-29 2022-04-07 富士フイルム株式会社 情報処理装置、情報処理方法、及びプログラム

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011229409A (ja) * 2010-04-23 2011-11-17 Nagoya Univ 細胞評価装置、インキュベータ、細胞評価方法、細胞評価プログラムおよび細胞の培養方法
WO2018105432A1 (ja) * 2016-12-06 2018-06-14 富士フイルム株式会社 細胞画像評価装置および細胞画像評価制御プログラム

Also Published As

Publication number Publication date
CN114127307A (zh) 2022-03-01
US12394170B2 (en) 2025-08-19
JPWO2021009906A1 (https=) 2021-01-21
JP7342950B2 (ja) 2023-09-12
US20220148282A1 (en) 2022-05-12
WO2021009906A1 (ja) 2021-01-21

Similar Documents

Publication Publication Date Title
CN114127307B (zh) 细胞图像解析方法和细胞图像解析装置
JP6981533B2 (ja) 細胞画像解析装置、細胞画像解析システム、学習データの生成方法、学習モデルの生成方法、学習データの生成プログラム、および、学習データの製造方法
US12039719B2 (en) System and method for detection and classification of objects of interest in microscope images by supervised machine learning
JP6791245B2 (ja) 画像処理装置、画像処理方法及び画像処理プログラム
US8000511B2 (en) System for and method of focusing in automated microscope systems
US9189677B2 (en) Recording medium having observation program recorded therein and observation apparatus
US8116543B2 (en) System for and method of intelligently directed segmentation analysis for automated microscope systems
EP4130843A9 (en) Microscope system, projection unit, and sperm sorting assistance method
US20030179916A1 (en) High-throughput cell identification and isolation method and apparatus
WO2010146802A1 (ja) 細胞塊の状態判別手法、この手法を用いた画像処理プログラム及び画像処理装置、並びに細胞塊の製造方法
WO2017150194A1 (ja) 画像処理装置、画像処理方法及びプログラム
JP6130801B2 (ja) 細胞領域表示制御装置および方法並びにプログラム
JP7815982B2 (ja) 学習支援システムおよび学習支援方法
US20240169523A1 (en) Cell counting method, machine learning model construction method and recording medium
JP2011004638A (ja) 受精卵観察の画像処理方法、画像処理プログラム及び画像処理装置
Liao et al. A clinical consensus-compliant deep learning approach to quantitatively evaluate human in vitro fertilization early embryonic development with optical microscope images
JP2012039929A (ja) 受精卵観察の画像処理方法、画像処理プログラム及び画像処理装置、並びに受精卵の製造方法
Niederlein et al. Image analysis in high content screening
WO2019171909A1 (ja) 画像処理方法、画像処理装置及びプログラム
JP7532754B2 (ja) 画像解析装置、細胞培養観察装置、画像解析方法、及びプログラム
EP4125065B1 (en) Image processing method and classification model construction method
CN121453734A (zh) 免疫荧光阴道分泌物自动检测仪的仪器
HK1215890B (zh) 用於查驗和分析細胞學標本的系統和方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant