CN114112619A - Detection method for female reproduction - Google Patents
Detection method for female reproduction Download PDFInfo
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- CN114112619A CN114112619A CN202111403329.9A CN202111403329A CN114112619A CN 114112619 A CN114112619 A CN 114112619A CN 202111403329 A CN202111403329 A CN 202111403329A CN 114112619 A CN114112619 A CN 114112619A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention relates to a detection method for female reproduction, which comprises the following steps: a1, placing the sampled sample in a sample tank in a treatment box, starting a water bath at the bottom of the sample tank, and carrying out constant-temperature heating treatment on the sample; a2, moving a liquid adding mechanism in the processing box to a position right above a sample to be detected corresponding to the sample tank, starting a peristaltic pump, and adding a detection reagent into a sample test tube in the sample tank through the liquid adding mechanism; a3, starting a shaking table mechanism in the treatment box, and uniformly mixing the sample and the reagent; a4, placing the uniformly mixed sample in a centrifuge in a processing box for centrifugal processing to finish the pretreatment of the sample; the detection method provided by the invention can automatically preprocess the secretion sample, thereby saving time and labor.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a detection method for female reproduction.
Background
Female reproductive system examinations typically include type-B ultrasonography, gynecological examinations, and general examinations for secretions such as infusorium, mold, bacteria, cleanliness, mycoplasma, chlamydia, gonococcal cultures, etc. The main purpose of the gynecological secretion routine examination is to examine whether the vagina has inflammation and the type of the inflammation, when the gynecological secretion does not conform to the normal expression, the gynecological secretion abnormality can occur, the cervical cancer is the current most serious disease in gynecology, the current incidence rate is extremely high, the trend of the youthfulness is higher and higher, the early symptoms of the cervical cancer are not obvious and can not be seen by eyes, and therefore, the cervical cancer can be confirmed only by medical detection, wherein the gynecological secretion routine detection is a common primary screening means, and therefore, the female reproductive examination has an important prejudgment function.
In gynecological clinical examination, in order to analyze dry chemical indexes and morphological indexes of a sample to be detected and obtain accurate quantitative and qualitative results, the sample to be detected is generally required to be pretreated, wherein the pretreatment mode of the vaginal secretion sample refers to that a cotton swab (or a flocked swab) is used for collecting a vaginal secretion sample, the swab is transferred into a treatment tube, and diluent is dripped to uniformly mix the vaginal secretion sample on the cotton swab to prepare vaginal secretion sample liquid for detection of an analyzer.
While the manual pre-treatment process for a complete vaginal secretion sample test generally requires 1-2 minutes, if the hospital sample size is too large, the operator will spend a lot of time and effort in the pre-treatment process.
Disclosure of Invention
The present invention is directed to solving the problems of the background art, and provides a method for detecting female reproduction, which can automatically pre-process a secretion sample, thereby saving time and labor.
The purpose of the invention is realized as follows:
a test method for female reproduction comprising a treatment chamber comprising the steps of:
a1, placing the sampled sample in a sample tank in a treatment box, starting a water bath at the bottom of the sample tank, and carrying out constant-temperature heating treatment on the sample;
a2, moving a liquid adding mechanism in the processing box to a position right above a sample to be detected corresponding to the sample tank, starting a peristaltic pump, and adding a detection reagent into a sample test tube in the sample tank through the liquid adding mechanism;
a3, starting a shaking table mechanism in the treatment box, and uniformly mixing the sample and the reagent;
and A4, placing the uniformly mixed sample in a centrifuge in a processing box for centrifugal processing to finish the pretreatment of the sample.
Preferably, the detecting system that following women's reproduction was used is adopted to the detection method, including handling the case, liquid feeding mechanism including the liquid feeding pipe that corresponds sample cell top and set up, the liquid feeding pipe locate in an inner tube, the outside swing joint of inner tube have an urceolus of coaxial setting, the urceolus locate on a lead screw, the inside wall of handling the case is connected at the both ends of lead screw, the urceolus through the nut with the lead screw adaptation along the length direction displacement of lead screw.
Preferably, the water bath is arranged on the shaking table mechanism, the shaking table mechanism comprises a bed body provided with a groove for accommodating the water bath, the bottom of the bed body is connected with two symmetrical swinging components, the swinging components comprise lifting lugs connected with the bottom of the bed body, one end of the lifting lug, which is far away from the bed body, is hinged with a guide rod, one end of the guide rod, which is far away from the lifting lug, is fixedly connected to the middle part of a swing arm, one end of the swing arm is hinged with a first rocker, one end of the first rocker, which is far away from the swing arm, is hinged with a first fixed seat, the other end of the swing arm is connected with a second rocker, one end of the second rocker, which is far away from the swing arm, is hinged to the second fixing seat, the first rocker rotates around the first fixed seat by taking the joint of the first rocker and the first fixed seat as a circle center, and the second rocker performs pendulum clock motion along the second fixing seat by taking the joint of the second rocker and the second fixing seat as a circle center.
Preferably, the sample tank is placed in a water bath tank, a circulating pump is connected to a pipeline of the water bath tank, and a temperature sensor for monitoring the temperature of water in the tank is further arranged in the water bath tank.
Preferably, the outer barrel is provided with a through hole communicated with the inner barrel, the top end of the liquid feeding pipe is connected with a pipeline connected with the peristaltic pump, the pipeline penetrates through the through hole to be connected with the peristaltic pump, and the top end of the outer barrel is provided with a driving motor used for driving the inner barrel to move relative to the outer barrel.
Preferably, a guide rail parallel to the centrifuge and the shaking table mechanism is arranged in the processing box and between the centrifuge and the shaking table mechanism, the guide rail is connected with a taking and placing mechanism, and the taking and placing mechanism moves along the guide rail to take out and place samples at different positions in the sample tank into the centrifuge.
Preferably, get and put mechanism include the stand through slider sliding connection guide rail, the top fixedly connected with bevel gear of stand one, the periphery cover on stand top is equipped with relative stand pivoted sleeve, the lateral wall fixedly connected with one and sleeve vertically L type curved boom of sleeve one side, telescopic one end is kept away from to the curved boom is equipped with curved boom vertically, columniform fixed cover, fixed cover in-connection has one to be the cantilever beam of L type.
Preferably, one end of the suspension beam is connected with a bevel gear II which is meshed and matched with the bevel gear I, and the other end of the suspension beam is connected with a mechanical claw for taking a sample.
Preferably, the water bath adopts constant temperature control, the bottom of water bath be equipped with the electric bar that is used for heating, temperature sensor adopt PT100 temperature sensor.
Preferably, the detection system also comprises an image acquisition unit for acquiring and processing the centrifuged sample and a control unit, the control unit is used for controlling the stepping motors in all mechanisms and collecting and data temperature signal data, the image acquisition unit comprises a microscope, a CCD camera and a microcontroller, the microcontroller comprises a parameter setting module, an automatic focusing module, an image acquisition module and an image splicing module, the microscope moves and focuses according to a set scanning path through the parameter setting module and the automatic focusing module, and controls the CCD camera to automatically collect the sample cells through the image collecting module, after the collected cell images identify abnormal cells through the microprocessor, the image splicing module is used for splicing the areas with abnormal pathological cells again, so that a doctor can conveniently recheck the identification result.
Preferably, the image stitching process of the image stitching module includes the following steps:
s1, estimating relative positions, namely acquiring an overlapping area between images by using a phase correlation method, and estimating the relative positions of two images to be spliced;
s2, extracting features, namely performing affine transformation on the image, then reconstructing a scale space of an SURF algorithm, improving the robustness of the SURF algorithm on the viewpoint change, increasing the extracted feature quantity, and then extracting the image features of a rough overlapping area;
and S3, image registration and image fusion, wherein the image registration is carried out based on the Euclidean distance of the extracted features, the weighted average fusion is adopted to eliminate the gaps between the images, and the images are fused.
Preferably, the cell images acquired under the microscope have different quality, the cells in some images are adhered and stacked seriously, the human eyes are difficult to distinguish easily, and the capabilities of network feature extraction and network generalization are improved through random spatial sampling pooling, specifically, the abnormal cell extraction process of the image acquisition module is as follows:
1) scaling the pixels of each collected image to 416 × 416, and inputting a feature extraction network according to 3 color channels corresponding to RGB;
2) firstly, an input sample cell image passes through a convolution layer with the size of 3 x 3, then cell image features are extracted through 2 residual blocks and 3 dense connecting blocks, each block is connected by the convolution layer with the size of 3 x 3 and S3Pool, wherein the S3Pool realizes random down-sampling with the window size of 2 x 2;
3) the 2 residual blocks which pass through are composed of zero filling layers and convolution layer cascade residual units, wherein the residual units are two convolution kernels of 1 x 1 and 3 x 3 under a residual structure, the first residual block comprises 1 residual unit, and the second residual block comprises 2 residual units;
4) the 3 dense convolution blocks which pass through are composed of zero padding layers, convolution layers, S3Pool and dense connection units, wherein the dense connection units are 1 x 1 and 3 x 3 convolution kernels under dense connection, the first dense convolution block and the second dense convolution block comprise 8 dense connection units, and the third dense convolution block comprises 4 dense connection units.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the female reproduction detection method provided by the invention, a sample is placed in a sample tank, a water bath tank can be used for heating the sample, a liquid adding mechanism can be used for diluting the sample and detecting a reagent, a shaking table mechanism can be used for uniformly mixing the sample, the pretreated sample is placed in a centrifuge through a taking and placing mechanism for centrifugal treatment, the collected secretion sample is uniformly mixed, the secretion sample is automatically diluted and uniformly mixed through a plurality of mechanisms, and manual operation is not needed, so that the time is saved and the labor is saved.
2. The invention provides a detection method for female reproduction, wherein a shaking table component is connected with a shaking table motor shaft, a bed body rotates together through the shaking table component, meanwhile, two position sensors are arranged at the lower position of the shaking table motor, a rocking bar II makes a pendulum clock reciprocating motion while the rocking bar I rotates for a circle, and the rocking bar I and the rocking bar II drive the connecting part of a guide rod and a lifting lug to make an elliptic motion under the action of the rocking bar I and the rocking bar II, so that the bed body swings within a certain amplitude range, and a sample and a reagent are promoted to regularly swing.
3. According to the female reproduction detection method provided by the invention, the sample added with the detection reagent is placed in the centrifuge for centrifugation through the taking and placing mechanism, so that the labor intensity of workers is reduced.
Drawings
Fig. 1 is a schematic view of a detection system of the detection method for female reproduction of the present invention.
Fig. 2 is a schematic view of a liquid adding mechanism of the detection method for female reproduction.
FIG. 3 is a schematic view of a sample cell of the test method for female reproduction according to the present invention.
Fig. 4 is a schematic view of a water bath for a female reproductive detection method of the present invention.
FIG. 5 is a schematic view of a cradle mechanism of the test method for female reproduction according to the present invention.
Fig. 6 is a schematic view of a pick-and-place mechanism of the detection method for female reproduction of the present invention.
In the figure: 1. a treatment tank; 2. a liquid adding mechanism; 3. a sample tank; 4. a water bath; 5. a centrifuge; 6. a guide rail; 7. a pick and place mechanism; 8. a circulation pump; 9. a temperature sensor; 10. a bed body; 11. a groove; 12. lifting lugs; 13. a first rocker; 14. a first fixed seat; 15. a guide bar; 16. swinging arms; 17. a rocker II; 18. a second fixed seat; 19. an outer cylinder; 20. an inner barrel; 21. a liquid feeding pipe; 22. a screw rod; 23. a through hole; 24. a column; 25. a sleeve; 26. a first bevel gear; 27. bending the arm; 28. a second bevel gear; 29. fixing a sleeve; 30. a suspension beam; 31. and (4) a mechanical claw.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by those skilled in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
With reference to fig. 1, a method for detecting female reproduction, comprising a treatment chamber 1, comprises the following steps:
a1, placing the sampled sample in a sample tank 3 in a treatment box 1, starting a water bath 4 at the bottom of the sample tank 3, and carrying out constant-temperature heating treatment on the sample;
a2, moving the liquid adding mechanism 2 in the processing box 1 to a position right above the sample tank 3 corresponding to the sample to be detected, starting a peristaltic pump, and adding the detection reagent into the sample test tube in the sample tank 3 through the liquid adding mechanism 2;
a3, starting a shaking table mechanism in the treatment box 1, and uniformly mixing the sample and the reagent;
and A4, placing the uniformly mixed sample in a centrifuge 5 in a processing box 1 for centrifugal processing to finish the pretreatment of the sample.
The sample pretreatment adopts an automatic process, and after corresponding parameters are set by workers, the system can automatically run to dilute, mix and centrifuge the sample, so that the detection efficiency is improved, and the labor intensity of the workers is reduced.
Example 2
With reference to fig. 1, a detection system for female reproduction comprises a processing box 1 for processing a sample to be detected, wherein a display screen is arranged on the processing box 1.
With reference to the figures 3 and 4, the processing box 1 is provided with a sample tank 3 for placing samples and a water bath tank 4 for heating the sample in a water bath, a test tube rack for limiting sample test tubes is arranged in the sample tank 3, the sample tank 3 is arranged in the water bath tank 4, the water bath tank 4 is connected with a circulating pump 8 through a pipeline, the water bath tank 4 is also provided with a temperature sensor 9 for monitoring the water temperature in the tank, the water bath tank is formed by welding stainless steel plates, the outside is wrapped by heat-insulating materials to prevent the heat loss of the liquid in the water bath tank, and simultaneously isolate the influence of the external environment temperature on the water bath tank, the left end and the right end of the water bath tank are respectively provided with water outlets, the two water outlets are connected with the circulating pump through silica gel tubes to form a closed circulating water path, thereby ensuring the stable flow of the liquid in the water bath tank, and two spirally installed temperature sensors are arranged near the water outlets in the water bath tank, the two temperature sensors are arranged for detecting the multipoint temperature of the liquid in the water bath tank, and then the two temperature values are compared, so that whether the temperature of the liquid in the water bath tank is uniform or not can be judged; the heating equipment of the water bath adopts a common electric heating rod, the heating power of the electric heating rod is controlled by regulating and controlling input voltage and current to change the heating quantity, so as to achieve the control purpose of keeping the temperature environment in the water bath constant, meanwhile, in order to make the temperature in the water bath uniform, two sides in the inner part of the bath body are respectively provided with a water outlet port, then the liquid in the water bath is in a flowing state through a circulating pump, and temperature sensors are also arranged at two positions in the bath body so as to carry out multi-point temperature detection, thereby judging the uniformity and stability of the temperature in the water bath body.
With reference to fig. 2, the top of sample cell 3 be equipped with the liquid feeding mechanism 2 that is used for the sample dropwise add reagent, liquid feeding mechanism 2 including the liquid feeding pipe 21 that corresponds the sample cell 3 top and set up, liquid feeding pipe 21 locate in an inner tube 20, the outside swing joint of inner tube 20 have an urceolus 19 of coaxial setting, urceolus 19 locate on a lead screw 22, the inside wall of processing case 1 is connected at the both ends of lead screw 22, urceolus 19 along the length direction displacement of lead screw 22 through the nut with the adaptation of lead screw 22, urceolus 19 on be equipped with the through-hole 23 with inner tube 20 intercommunication, the top of liquid feeding pipe 21 be connected with the pipeline of being connected with the peristaltic pump, the pipeline pass through-hole 23 and be connected with the peristaltic pump, the 19 top of urceolus be equipped with the driving motor who is used for driving the relative urceolus 19 displacements of inner tube 20, in the liquid feeding process, in order to ensure the accuracy of the liquid adding amount, a peristaltic pump device is selected, and a grating encoder is additionally arranged in a driving stepping motor of the peristaltic pump, so that closed-loop control is formed on the stepping motor, and the accuracy of the liquid adding position and the liquid adding amount is improved.
Referring to fig. 5, the water bath 4 is disposed on a rocking bed mechanism, the rocking bed mechanism includes a bed body 10 having a groove 11 for accommodating the water bath 4, the bottom of the bed body 10 is connected with two symmetrical rocking assemblies, each rocking assembly includes a lifting lug 12 connected to the bottom of the bed body 10, one end of the lifting lug 12 away from the bed body 10 is hinged to a guide rod 15, one end of the guide rod 15 away from the lifting lug 12 is fixedly connected to the middle of a swing arm 16, one end of the swing arm 16 is hinged to a rocker 13, one end of the rocker 13 away from the swing arm 16 is hinged to a first fixing seat 14, the other end of the swing arm 16 is connected to a second rocker 17, one end of the rocker 17 away from the swing arm 16 is hinged to a second fixing seat 18, the first rocker 13 makes a circular motion around the first fixing seat 14 with the connection point of the first rocker 13 and the first fixing seat 14 as a circle center, the rocking bar II 17 is in pendulum clock motion along the fixing seat II 18 by taking the joint of the rocking bar II and the fixing seat II 18 as a circle center, the rocking bar assembly is connected with a shaft of a rocking bed motor, the bed body rotates together through the rocking bar assembly, meanwhile, two position sensors are arranged at the position below the rocking bed motor, the rocking bar II performs pendulum clock reciprocating motion while the rocking bar I rotates for a circle, the rocking bar drives the joint of the guide rod and the lifting lug to perform elliptic motion under the action of the rocking bar I and the rocking bar II, the bed body swings within a certain amplitude range, and samples and reagents are enabled to regularly shake and mix.
Example 2
With reference to fig. 1 and fig. 6, the processing box 1 is further provided with a centrifuge 5 parallel to the shaking table mechanism, the processing box 1, a guide rail 6 parallel to the centrifuge 5 and the shaking table mechanism is arranged between the centrifuge 5 and the shaking table mechanism, the guide rail 6 is connected with a pick-and-place mechanism 7, and the pick-and-place mechanism 7 realizes the pick-up and the placement of samples at different positions in the sample tank in the centrifuge 5 through the displacement on the guide rail.
The pick-and-place mechanism 7 comprises an upright post 24 which is connected with the guide rail 6 in a sliding way through a sliding block, a bevel gear I26 is fixedly connected at the top end of the upright post 24, a sleeve 25 which rotates relative to the upright post 24 is sleeved on the periphery of the top end of the upright post 24, an L-shaped bent arm 27 which is vertical to the sleeve 25 is fixedly connected with the outer side wall of one side of the sleeve 25, the end of the bent arm 27 far away from the sleeve 25 is provided with a cylindrical fixing sleeve 29 perpendicular to the bent arm 27, an L-shaped suspension beam 30 is connected in the fixed sleeve 29, one end of the suspension beam 30 is connected with a bevel gear II 28 which is meshed and matched with the bevel gear I26, the other end of the suspension beam 30 is connected with a mechanical claw 31 used for taking a sample, the mechanical claw adopts a mechanical arm or mechanical arm equipment in the prior art, two mutually symmetrical clamping grooves used for clamping a test tube are formed in the end part of the mechanical claw, and a first bevel gear and a second bevel gear are driven by a stepping motor.
And the second bevel gear is in meshing transmission along the tooth grooves in a certain radian range on the first bevel gear, the suspension beam connected with the second bevel gear drives the sleeve to rotate along the circumferential direction of the upright column through the bent arm, the cantilever is horizontally placed or lifted along with the change of the moving position of the second bevel gear, the vertical arm of the L-shaped cantilever is horizontally placed or lifted, the reciprocating motion of the mechanical claw in a certain range is realized, a sample is taken out from the sample groove, and the sample is placed in a centrifuge.
Example 3
The detection system also comprises an image acquisition unit for acquiring and processing the centrifuged sample and a control unit, the control unit is used for controlling the stepping motors in all mechanisms and collecting and data temperature signal data, the image acquisition unit comprises a microscope, a CCD camera and a microcontroller, the microcontroller comprises a parameter setting module, an automatic focusing module, an image acquisition module and an image splicing module, the microscope moves and focuses according to a set scanning path through the parameter setting module and the automatic focusing module, and controls the CCD camera to automatically collect the sample cells through the image collecting module, after the collected cell images identify abnormal cells through the microprocessor, the image splicing module is used for splicing the areas with abnormal pathological cells again, so that a doctor can conveniently recheck the identification result.
The image splicing process of the image splicing module comprises the following steps:
s1, estimating relative positions, namely acquiring an overlapping area between images by using a phase correlation method, and estimating the relative positions of two images to be spliced;
s2, extracting features, namely performing affine transformation on the image, then reconstructing a scale space of an SURF algorithm, improving the robustness of the SURF algorithm on the viewpoint change, increasing the extracted feature quantity, and then extracting the image features of a rough overlapping area;
and S3, image registration and image fusion, wherein the image registration is carried out based on the Euclidean distance of the extracted features, the weighted average fusion is adopted to eliminate the gaps between the images, and the images are fused.
The high-speed and high-precision splicing of the microscope images is realized, and the purposes of observing, exchanging, storing and establishing a microscope image database are achieved.
The cell image quality that gathers under the microscope is uneven, and cell adhesion, the pile-up condition are serious in some images, and the eyes all are difficult to easily distinguish, through the pooling of random space sampling, promote the ability of network characteristic extraction and network generalization, and specifically, the process of the unusual cell extraction of image acquisition module is as follows:
1) scaling the pixels of each collected image to 416 × 416, and inputting a feature extraction network according to 3 color channels corresponding to RGB;
2) firstly, an input sample cell image passes through a convolution layer with the size of 3 x 3, then cell image features are extracted through 2 residual blocks and 3 dense connecting blocks, each block is connected by the convolution layer with the size of 3 x 3 and S3Pool, wherein the S3Pool realizes random down-sampling with the window size of 2 x 2;
3) the 2 residual blocks which pass through are composed of zero filling layers and convolution layer cascade residual units, wherein the residual units are two convolution kernels of 1 x 1 and 3 x 3 under a residual structure, the first residual block comprises 1 residual unit, and the second residual block comprises 2 residual units;
4) the 3 dense convolution blocks which pass through are composed of zero padding layers, convolution layers, S3Pool and dense connection units, wherein the dense connection units are 1 x 1 and 3 x 3 convolution kernels under dense connection, the first dense convolution block and the second dense convolution block comprise 8 dense connection units, and the third dense convolution block comprises 4 dense connection units.
And the residual structure before outputting the cell image features extracted by the network is replaced by a dense convolution block, so that the feature graph can be better combined with the information of a front-layer network to fuse the features.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalents and substitutions made within the scope of the present invention should be included.
Claims (8)
1. A method of testing female reproduction, comprising: the detection method comprises the following steps:
a1, placing the sampled sample in a sample tank (3) in a treatment box (1), starting a water bath (4) at the bottom of the sample tank (3), and carrying out constant-temperature heating treatment on the sample;
a2, moving a liquid adding mechanism (2) in a processing box (1) to a position right above a sample to be detected corresponding to a sample tank (3), starting a peristaltic pump, and adding a detection reagent into a sample test tube in the sample tank (3) through the liquid adding mechanism (2);
a3, starting a shaking table mechanism in the treatment box (1) to uniformly mix the sample and the reagent;
a4, placing the uniformly mixed sample in a centrifuge (5) in a processing box (1) for centrifugal processing to finish the pretreatment of the sample.
2. A method of detecting female reproduction as claimed in claim 1, wherein: liquid feeding mechanism (2) including liquid feeding pipe (21) that corresponds sample cell (3) top setting, liquid feeding pipe (21) locate in an inner tube (20), the outside swing joint of inner tube (20) have urceolus (19) of a coaxial setting, urceolus (19) locate on a lead screw (22), the inside wall of handling case (1) is connected at the both ends of lead screw (22), urceolus (19) through the length direction displacement along lead screw (22) with the nut of lead screw (22) adaptation.
3. A method of detecting female reproduction as claimed in claim 1, wherein: the water bath (4) is arranged on the shaking table mechanism, the shaking table mechanism comprises a bed body (10) provided with a groove (11) for accommodating the water bath (4), the bottom of the bed body (10) is connected with two symmetrical swinging assemblies, each swinging assembly comprises a lifting lug (12) connected with the bottom of the bed body (10), one end, far away from the bed body (10), of each lifting lug (12) is hinged with a guide rod (15), one end, far away from the lifting lug (12), of each guide rod (15) is fixedly connected to the middle of a swinging arm (16), one end of each swinging arm (16) is hinged with a first rocker (13), one end, far away from the swinging arm (16), of each first rocker (13) is hinged to a first fixing seat (14), the other end of each swinging arm (16) is connected with a second rocker (17), one end, far away from the swinging arm (16), of each rocker (17) is hinged to a second fixing seat (18), the rocker I (13) makes circular motion around the fixing seat I (14) by taking the joint of the rocker I and the fixing seat I (14) as a circle center, and the rocker II (17) makes pendulum clock motion along the fixing seat II (18) by taking the joint of the rocker II and the fixing seat II (18) as a circle center.
4. A method of detecting female reproduction as claimed in claim 1, wherein: the sample tank (3) is placed in the water bath tank (4), the water bath tank (4) is connected with a circulating pump (8) through a pipeline, and a temperature sensor (9) used for monitoring the water temperature in the tank is further arranged inside the water bath tank (4).
5. A method of detecting female reproduction as claimed in claim 2, wherein: the outer barrel (19) is provided with a through hole (23) communicated with the inner barrel (20), the top end of the liquid adding pipe (21) is connected with a pipeline connected with the peristaltic pump, the pipeline penetrates through the through hole (23) to be connected with the peristaltic pump, and the top end of the outer barrel (19) is provided with a driving motor used for driving the inner barrel (20) to move relative to the outer barrel (19).
6. A method of detecting female reproduction as claimed in claim 1, wherein: the processing box (1) in, be equipped with between centrifuge (5) and the shaking table mechanism with guide rail (6) parallel with centrifuge (5) and shaking table mechanism, guide rail (6) on be connected with one and get and put mechanism (7), get and put mechanism (7) along guide rail (6) removal with the sample of the different positions in sample groove (3) take out and place in centrifuge (5).
7. The method of claim 6, wherein the step of detecting comprises: get and put mechanism (7) include stand (24) through slider sliding connection guide rail (6), the top fixedly connected with bevel gear (26) of stand (24), the periphery cover on stand (24) top is equipped with relative stand (24) pivoted sleeve (25), the lateral wall fixedly connected with one of sleeve (25) one side and sleeve (25) vertically L type curved arm (27), the one end that sleeve (25) were kept away from in curved arm (27) is equipped with curved arm (27) vertically, columniform fixed cover (29), fixed cover (29) in-connection has a cantilever beam (30) that is the L type.
8. The method of claim 7, wherein the step of detecting comprises: one end of the suspension beam (30) is connected with a bevel gear II (28) which is meshed and matched with the bevel gear I (26), and the other end of the suspension beam (30) is connected with a mechanical claw (31) used for taking a sample.
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