CN114107409B - Method for treating rice bran meal and product - Google Patents

Method for treating rice bran meal and product Download PDF

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CN114107409B
CN114107409B CN202111408567.9A CN202111408567A CN114107409B CN 114107409 B CN114107409 B CN 114107409B CN 202111408567 A CN202111408567 A CN 202111408567A CN 114107409 B CN114107409 B CN 114107409B
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supernatant
bran meal
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CN114107409A (en
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宣丽
李万军
杨华
马金鹏
张媛媛
徐波
杜翠荣
李艳辉
甄广田
高虹
吕美
何欢
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Liaoning Complete Bio Technology Co ltd
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Abstract

The invention discloses a method for treating rice bran meal and a product thereof, wherein the content of anti-nutritional factors in the rice bran meal is reduced by extracting phytic acid and soluble non-starch polysaccharide, and crude calcium phytate, a feeding microecological regulator, rice bran polysaccharide and rice bran protein peptide are prepared. The beneficial effects of the invention are as follows: the process produces various high-added-value products while producing the rice bran meal with low anti-nutritional factors by comprehensively utilizing the rice bran meal, has no waste emission in the whole process, and truly realizes green and safe high-value application production of the rice bran meal.

Description

Method for treating rice bran meal and product
Technical Field
The invention relates to a deep processing and comprehensive utilization process of rice bran meal, in particular to a method for producing rice bran meal with low anti-nutritional factors by extracting phytic acid and soluble non-starch polysaccharide.
Background
In order to adapt to the new situation that the supply and demand of bulk feed raw materials are tight, the utilization efficiency of raw materials is improved, a novel daily ration formula structure is built based on national conditions, the promotion of corn and soybean meal reduction substitution is accelerated, and the development of unconventional feed resources becomes a hot spot of current research. The rice bran meal is a byproduct of rice processing, is rich in resources and low in price, contains higher nutritional ingredients, and can be used as one of raw materials for reducing and replacing corn and bean pulp. However, because the rice bran meal has higher content of non-starch polysaccharide, phytic acid and other anti-nutritional factors, the overhigh dosage often affects the digestion of the whole nutrients of the diet, so that the production performance of livestock and poultry is reduced, and the proportion is limited during application.
Phytic acid is an important anti-nutritional factor in rice bran meal, and the proportion is about 7%. Because phytic acid is relatively stable in nature, the phytic acid is difficult to damage by physical treatment methods such as heating and the like, and the corresponding enzyme system is not digested and degraded in animal bodies. At present, phytase is mainly added into feed to degrade phytic acid in animal bodies, and the feed has extremely wide application. However, the effect of enzyme preparations in vivo can only be assessed in reverse by the performance of the feeding test production performance. A meta-analysis by Rosenfelder-Kuon et al 2020 shows that with phytase addition, the average phosphorus digestibility of pigs was stabilized at 65% although the phytic acid recovery was low in the pig manure. Another study by this authors (2020) also showed that with high levels of phytase addition, pig full gut phosphorus digestibility was not more than 60%. Therefore, new ways to reduce the nutritional resistance of phytic acid are needed.
Non-starch polysaccharides are another important class of antinutritional factors in rice bran meal, and are classified into soluble non-starch polysaccharides and insoluble non-starch polysaccharides according to solubility. Among these, the soluble non-starch polysaccharide is rice bran polysaccharide having good functional properties in the food field. At present, a great deal of scientific researches at home and abroad have proved that rice bran polysaccharides have various biological activities, such as apoptosis and cell cycle blocking effects on various cancer cells; antibacterial, antiviral, and immunity regulating; reducing blood glucose and regulating sugar metabolism; radiation resistance, liver protection, etc. However, in the feed, the feed has the adverse effects of increasing the viscosity of chyme, increasing the value of harmful bacteria and the like, and becomes an anti-nutritional factor.
In the prior art, comprehensive utilization of the rice bran meal for removing the anti-nutritional factors in the rice bran meal is not reported yet. The invention prepares the rice bran meal with low anti-nutritional factors by extracting phytic acid and soluble non-starch polysaccharide in the rice bran meal, and simultaneously prepares various products such as crude calcium phytate, liquid feeding microecological regulator, rice bran polysaccharide, rice bran protein peptide and the like, thereby realizing conversion and increment of the rice bran meal.
Disclosure of Invention
The invention aims to provide a method for producing rice bran meal with low anti-nutritional factors by comprehensively utilizing rice bran meal and considering the requirements of food and feed fields, so that the method is capable of fully utilizing resources to the maximum extent, has low process energy consumption, is green and safe, accords with the development strategy of grain saving and emission reduction in China at present, and also accords with the development idea of prolonging the by-product processing industry chain and driving comprehensive benefit of the by-products to be comprehensively improved.
The technical scheme of the invention comprises the following steps:
(1) Acid leaching: adding water into rice bran meal according to a solid-liquid mass ratio of 1 (3-5), and then adjusting the pH value of the feed liquid to 3.6-3.9 by using 2-6 mol/L hydrochloric acid, and soaking for 50-70 min;
(2) And (3) centrifuging: centrifuging the acid leaching solution at the rotating speed of 4000-5000 r/min for 5-10 min, collecting supernatant A, and allowing residues to enter the next step;
(3) Washing residues: adding water with the mass 1-2 times of that of the rice bran meal into the residue, stirring uniformly, centrifuging at the rotating speed of 4000-5000 r/min for 5-10 min, and mixing the supernatant with the A. Repeatedly washing the residues for one time according to the step, enabling the supernatant mixed solution A to enter the next step, and enabling residues to enter the step (6);
(4) Regulating the pH value of the supernatant fluid A to 4.0-4.5 by using calcium hydroxide with the mass fraction of 8-12%, regulating the pH value to 7.5-7.6 by using sodium hydroxide with the mass fraction of 8-12%, standing for 1-2 h, centrifuging at the rotating speed of 4000-5000 r/min for 8-12 min to obtain a supernatant fluid B, precipitating at 75-85 ℃ and drying to obtain crude calcium phytate;
(5) Fermentation: sterilizing the supernatant B at 110-121 ℃ for 10-20 min, cooling to room temperature, adding EM bacteria accounting for 2-4% of the mass of rice bran meal, sealing, and fermenting at room temperature for 3-5 d to obtain a liquid micro-ecological regulator for feeding;
(6) Extracting soluble non-starch polysaccharide: transferring the residue obtained in the step (3) into hydraulic oscillation equipment, adding water with the mass of 10-15 times of that of rice bran meal, stirring uniformly, adjusting the pH of the feed liquid to 7.5-8.0 by using sodium hydroxide, adding alkaline protease (the enzyme activity is more than or equal to 100U/mg) with the mass of 1-2% of that of the rice bran meal, and carrying out oscillation extraction for 10-20 min;
(7) And (3) centrifuging: centrifuging the oscillating liquid at the rotating speed of 4000-5000 r/min for 10-20 min to obtain supernatant C, and drying residues at 70-80 ℃ to obtain rice bran meal with low anti-nutritional factors;
(8) Concentrating: concentrating the supernatant C under reduced pressure at the temperature of 75-85 ℃ under the pressure of-0.09 to-0.1 MPa, wherein the ratio of the volume of the concentrated solution to the volume of the supernatant C is 1:8-1:10;
(9) Alcohol precipitation: adding absolute ethyl alcohol into the concentrated solution to ensure that the final volume concentration of the ethyl alcohol is 50-70%, and refrigerating for 12-16 hours at 2-6 ℃;
(10) And (3) centrifuging: centrifuging the refrigerated polysaccharide alcohol precipitation solution at the rotating speed of 4000-5000 r/min for 10-15 min to obtain supernatant D, and drying the precipitate at 65-75 ℃ to obtain rice bran polysaccharide;
(11) Recovering ethanol: concentrating the supernatant D under reduced pressure at the temperature of 50-60 ℃ and the pressure of minus 0.09-minus 0.1MPa, and drying the concentrated solution under the temperature of 55-65 ℃ and minus 0.09-minus 0.1MPa after the ethanol is thoroughly recovered to obtain the rice bran protein peptide.
The inventor designs the process carefully, and all the steps are closely connected and are buckled, so that the safe and green comprehensive development and utilization of rice bran meal are realized. The invention has the advantages that the phytic acid and the chelated metal ions thereof in the rice bran meal are extracted by adopting an acid leaching method, so that the ash content of the rice bran polysaccharide extracted subsequently can be reduced from more than 20% to less than 2%, and the purity of the polysaccharide is improved; in addition, the protein content in the rice bran polysaccharide can be reduced from more than 25% to less than 10% by combining the hydraulic oscillation with the enzymatic extraction, so that the purity of the polysaccharide is further improved. The invention also has the advantages that the protein separated from rice bran polysaccharide mainly exists in the form of peptide, so that a protein peptide product can be formed, and the conversion value-added benefit is further improved.
The order of acid leaching and soluble non-starch polysaccharide extraction cannot be changed, otherwise the ash content in the subsequently prepared polysaccharide product is too high, which reduces the polysaccharide purity.
Further, the pH range of the acid leaching in the step (1) is 3.6-3.9, and the value is lower than the pH range, so that the residual amount of the phytic acid in the rice bran meal with low anti-nutritional factors prepared later is more than 0.4%, and the phytic acid is not completely removed.
Further, in the step (4), the yield of the calcium phytate is 13-16%, and the yield of the phytic acid is more than 80%.
Further, in the step (5), after fermentation, the pH of the supernatant B is reduced from 7.5-7.6 to 3.8-4.2, and the beneficial bacteria content comprises lactobacillus: 4.0 to 8.3X10 7 CFU/g, streptococcus thermophilus: 1.2 to 3.5X10 7 CFU/g, bifidobacteria: 1.4 to 3.9X10 7 CFU/g. Can be used asIs added into feed for direct feeding.
Further, in the step (7), the rice bran meal with low anti-nutritional factors (calculated on a dry basis) has the phytic acid content less than 0.1%, the soluble non-starch polysaccharide content less than 0.7%, the fiber content of 60-62%, the starch content of 18-22% and the protein content of 15-19%.
Further, in the step (10), the yield of the rice bran polysaccharide is 6-8%, and the purity of the rice bran polysaccharide is more than 70%.
Further, in the step (11), the yield of the rice bran protein peptide is 7-9%, the protein content is more than 80%, and the small peptide with the protein content of less than 1000Da accounts for more than 30%.
In the above method, the principle of hydraulic oscillation is similar to cavitation of ultrasonic waves, but since the industrialized use of ultrasonic waves is limited, we find an alternative technology, and the method has the following advantages: 1. can be directly amplified for production; 2. the treatment time is short, and the extraction rate of polysaccharide is reduced by 0.4-1% after more than 25min in experiments, so that the oscillation time is controlled within 25 min; 3. the technology is supplemented with enzyme, so that the purity of the polysaccharide can be improved by more than 30%.
In summary, the invention has the advantages that four high added value products of calcium phytate, rice bran protein peptide, rice bran polysaccharide and liquid feeding microecological regulator are obtained while reducing the content of anti-nutritional factors in rice bran meal. Firstly, the acid leaching process of the invention reduces about 12% of ash content in rice bran meal to below 2%, about 7% of phytic acid to below 0.1%, and reduces ash content of rice bran polysaccharide extracted subsequently from above 20% to below 2%; secondly, the invention fully utilizes the supernatant after calcium phytate precipitation. The partial supernatant contains about 10% of sugar by mass of rice bran meal and about 8% of soluble protein in total protein of the rice bran meal, and the partial supernatant is used as a liquid feeding microecological regulator through a fermentation technology because of acid-base neutralization and also contains a part of salt to be directly discharged and pollute the environment, and a fermentation product with rich beneficial bacteria content can be obtained without adding carbon and nitrogen sources additionally; thirdly, simultaneously obtaining high-purity rice bran polysaccharide and rice bran protein peptide products with high small peptide content by hydraulic oscillation assisted with enzyme treatment; finally, the rice bran meal with low anti-nutritional factors obtained by the invention eliminates the anti-nutritional effect of the rice bran meal, retains most dietary fibers in the rice bran meal, has good health care function, and can be widely added into feeds. The invention not only makes full and effective use of various nutrition and health care components in the rice bran meal, but also has the advantages of short production period, high extraction rate, cost and energy consumption saving, simple operation, no waste discharge and realization of high-value application production of the rice bran meal.
Detailed Description
The following examples are intended to illustrate the invention and are not intended to be limiting.
Example 1
Adding water into rice bran meal according to a solid-liquid mass ratio of 1:3 for batching, then adjusting the pH of feed liquid to 3.6 by using 2mol/L hydrochloric acid, soaking for 50min, centrifuging at a rotating speed of 4000r/min for 5min, and collecting supernatant A;
washing residues: adding water 1 time of the mass of the rice bran meal into the residue, stirring uniformly, centrifuging at 4000r/min for 5min, and mixing the supernatant with the A. And (3) repeatedly washing slag for one time according to the step, regulating the pH value of the supernatant mixed solution A to 4.0 by using 8% of calcium hydroxide by mass fraction, regulating the pH value of the supernatant mixed solution A to 7.5 by using 8% of sodium hydroxide by mass fraction, standing for 1h, centrifuging at the rotating speed of 4000r/min for 8min to obtain a supernatant B, and drying the precipitate at the temperature of 75 ℃ to obtain crude calcium phytate. The yield of the calcium phytate is determined to be 13.2%, and the phosphorus content is determined to be 8.5%.
Yield of phytic acid: the total phosphorus content of the batch of rice bran meal is 1.55%, the content of the phytic acid phosphorus is 1.39%, and the phytic acid yield is 80.7%.
Sterilizing supernatant B obtained after calcium phytate precipitation at 121deg.C for 10min, cooling to room temperature, adding EM strain (10 g/branch) accounting for 2% of the mass of rice bran meal (Bai Yibao biological technology Co., ltd.), sealing, and fermenting at room temperature for 5d to obtain the liquid microecological regulator for feeding.
The pH of the microecological regulator is 4.2, and the beneficial bacteria content comprises lactobacillus: 4.0X10 7 CFU/g, streptococcus thermophilus: 1.2X10 7 CFU/g, bifidobacteria: 1.4X10 7 CFU/g. Can be used as a microecological regulator for liquid feed to be added into feed for direct feeding.
Transferring the residue obtained by acid leaching and centrifuging to hydraulic oscillation equipment (North Liaoning environmental technology Co., ltd.) and adding water 10 times the mass of rice bran meal, stirring uniformly, adjusting pH of the feed liquid to 7.5 with sodium hydroxide, adding alkaline protease (enzyme activity: 100U/mg) 1% of the mass of rice bran meal, oscillating and extracting for 10min, centrifuging at 4000r/min for 10min, obtaining supernatant C, and oven drying the residue at 70deg.C to obtain rice bran meal with low anti-nutritional factors.
According to measurement, the rice bran meal with low anti-nutritional factors (calculated on a dry basis) contains 0.08% of phytic acid, 0.63% of soluble non-starch polysaccharide, 60.2% of insoluble dietary fiber, 18.4% of starch, 18.7% of protein, 0.68% of fat and 1.31% of ash.
Concentrating the supernatant C under reduced pressure at-0.09 MPa and 75 ℃ to obtain a ratio of the volume of the concentrated solution to the volume of the supernatant C of 1:8, adding absolute ethanol into the concentrated solution to ensure that the final volume concentration of the ethanol is 50%, refrigerating for 12h at 2 ℃, centrifuging at 4000r/min for 10min, obtaining a supernatant D, and drying the precipitate at 65 ℃ to obtain rice bran polysaccharide.
The rice bran polysaccharide yield was determined to be 6.3% and polysaccharide content was determined to be 73.4%.
Concentrating the supernatant D under reduced pressure at-0.09 MPa and 50deg.C, and vacuum drying the concentrated solution at-0.09 MPa and 55deg.C to obtain rice bran protein peptide.
The yield of the rice bran protein peptide is 7.1%, the protein content is 81.7%, and the small peptide with the protein content below 1000Da accounts for 36.6%.
The molecular weight distribution of the protein peptides is shown in the following table:
TABLE 1 example 1 molecular weight distribution of Rice bran protein peptides
Example 2
Adding water into rice bran meal according to a solid-liquid mass ratio of 1:4 for batching, then adjusting the pH of feed liquid to 3.75 by using 4mol/L hydrochloric acid, soaking for 60min, centrifuging at a rotating speed of 4000r/min for 8min, collecting supernatant A, adding water with a mass 1.5 times that of the rice bran meal into residues, uniformly stirring, centrifuging at a rotating speed of 5000r/min for 8min, and mixing the supernatant with the A. And (3) repeatedly washing slag for one time according to the step, regulating the pH value of the supernatant mixed solution A to be 4.2 by using 10% of calcium hydroxide by mass fraction, regulating the pH value of the supernatant mixed solution A to be 7.55 by using 10% of sodium hydroxide by mass fraction, standing for 1.5h, centrifuging at the rotating speed of 4000r/min for 10min to obtain a supernatant B, and drying the precipitate at 80 ℃ to obtain crude calcium phytate. The yield of the calcium phytate is 14.6% and the phosphorus content is 8.1% by measurement.
Yield of phytic acid: the total phosphorus content of the batch of rice bran meal is 1.55%, the content of the phytic acid phosphorus is 1.39%, and the phytic acid yield is 85.1%.
Sterilizing supernatant B obtained after calcium phytate precipitation at 115 ℃ for 15min, cooling to room temperature, adding EM strain (10 g/branch of Bai Yibao biological technology Co., zhengzhou) accounting for 3% of the mass of rice bran meal, sealing, and fermenting at room temperature for 4d to obtain the liquid microecological regulator for feeding.
The pH of the microecological regulator is 4.0, and the beneficial bacteria content comprises lactobacillus: 6.1X10 times 7 CFU/g, streptococcus thermophilus: 2.3X10 7 CFU/g, bifidobacteria: 2.5X10 7 CFU/g. Can be used as a microecological regulator for liquid feed to be added into feed for direct feeding.
Transferring the residue obtained by acid leaching and centrifuging to hydraulic oscillation equipment (North Liaoning environmental technology Co., ltd.) and adding water 12 times the mass of rice bran meal, stirring uniformly, adjusting pH of the feed liquid to 7.8 with sodium hydroxide, adding alkaline protease (enzyme activity: 200U/mg) 1.5% of the mass of rice bran meal, oscillating and extracting for 15min, centrifuging at 4000r/min for 15min, obtaining supernatant C, and drying the residue at 75deg.C to obtain rice bran meal with low anti-nutritional factors.
According to measurement, the rice bran meal with low anti-nutritional factors (calculated on a dry basis) contains 0.05% of phytic acid, 0.44% of soluble non-starch polysaccharide, 61.2% of insoluble dietary fiber, 19.5% of starch, 17.3% of protein, 0.47% of fat and 1.04% of ash.
Concentrating the supernatant C under reduced pressure at-0.095 MPa and 80deg.C, wherein the ratio of the volume of the concentrated solution to the volume of the supernatant C is 1:9, adding absolute ethanol into the concentrated solution to make the final ethanol volume concentration be 60%, refrigerating at 4deg.C for 14h, centrifuging at 5000r/min for 12min to obtain supernatant D, and oven drying the precipitate at 70deg.C to obtain rice bran polysaccharide.
The rice bran polysaccharide yield was determined to be 6.9% and polysaccharide content was determined to be 75.7%.
Concentrating the supernatant D under reduced pressure at-0.095 MPa and 55deg.C, and vacuum drying the concentrated solution at-0.095 MPa and 60deg.C to obtain testa oryzae protein peptide.
The measurement shows that the yield of the rice bran protein peptide is 8.2%, the protein content is 84.1%, and the small peptide with the protein content below 1000Da accounts for 40.4%.
The molecular weight distribution of the protein peptides is shown in the following table:
TABLE 2 example 2 molecular weight distribution of Rice bran protein peptides
Example 3
Adding water into rice bran meal according to a solid-liquid mass ratio of 1:5 for batching, then adjusting the pH of feed liquid to 3.9 by using 6mol/L hydrochloric acid, soaking for 70min, centrifuging at a rotating speed of 5000r/min for 10min, collecting supernatant A, adding water with a mass which is 2 times that of the rice bran meal into residues, uniformly stirring, centrifuging at a rotating speed of 5000r/min for 10min, and mixing the supernatant A with the supernatant A. And (3) repeatedly washing slag for one time according to the step, regulating the pH value of the supernatant mixed solution A to 4.5 by using calcium hydroxide with the mass fraction of 12%, regulating the pH value to 7.6 by using sodium hydroxide with the mass fraction of 12%, standing for 2 hours, centrifuging at the rotating speed of 5000r/min for 12min to obtain a supernatant B, and drying the precipitate at the temperature of 85 ℃ to obtain crude calcium phytate. The yield of the calcium phytate is 15.8 percent and the phosphorus content is 7.8 percent.
Yield of phytic acid: the total phosphorus content of the batch of rice bran meal is 1.55%, the content of the phytic acid phosphorus is 1.39%, and the yield of the phytic acid is 88.7%.
Sterilizing supernatant B obtained after calcium phytate precipitation at 110deg.C for 20min, cooling to room temperature, adding EM strain (10 g/branch) accounting for 4% of the mass of rice bran meal (Bai Yibao biological technology Co., ltd.), sealing, and fermenting at room temperature for 3d to obtain the liquid microecological regulator for feeding.
The pH of the microecological regulator is 3.8, and the beneficial bacteria content comprises lactobacillus: 8.3X10 7 CFU/g, streptococcus thermophilus: 3.5X10 7 CFU/g, bifidobacteria: 3.9X10 7 CFU/g. Can be used as a microecological regulator for liquid feed to be added into feed for direct feeding.
Transferring the residue obtained by acid leaching and centrifuging to hydraulic oscillation equipment (North Liaoning environmental technology Co., ltd.) and adding 15 times of water to the rice bran meal, stirring uniformly, adjusting pH to 8.0 with sodium hydroxide, adding alkaline protease (enzyme activity: 200U/mg) 2% of the rice bran meal mass, oscillating and extracting for 20min, centrifuging at 5000r/min for 20min, collecting supernatant C, and oven drying the residue at 80deg.C to obtain rice bran meal with low anti-nutritional factor.
According to measurement, the rice bran meal with low anti-nutritional factors (on a dry basis) contains 0.02% of phytic acid, 0.43% of soluble non-starch polysaccharide, 61.4% of insoluble dietary fiber, 21.6% of starch, 15.2% of protein, 0.41% of fat and 0.94% of ash.
Concentrating the supernatant C under reduced pressure at-0.1 MPa and 85 deg.C, wherein the ratio of the volume of the concentrated solution to the volume of the supernatant C is 1:10, adding absolute ethanol into the concentrated solution to make the final ethanol volume concentration be 70%, refrigerating at 6 deg.C for 16h, centrifuging at 5000r/min for 15min to obtain supernatant D, and drying the precipitate at 75 deg.C to obtain rice bran polysaccharide.
The rice bran polysaccharide yield was determined to be 7.7% and polysaccharide content was determined to be 77.2%.
Concentrating the supernatant D under reduced pressure at-0.1 MPa and 60 ℃, and vacuum drying the concentrated solution at-0.1 MPa and 65 ℃ after the ethanol is thoroughly recovered to obtain the rice bran protein peptide.
The yield of the rice bran protein peptide is determined to be 8.8%, the protein content is 87.4%, and the small peptide with the protein content below 1000Da accounts for 41.2%.
The molecular weight distribution of the protein peptides is shown in the following table:
TABLE 3 example 3 molecular weight distribution of Rice bran protein peptides
The invention reduces the content of anti-nutritional factors in rice bran meal by extracting phytic acid and soluble non-starch polysaccharide, and simultaneously prepares crude calcium phytate, liquid feeding microecological regulator, rice bran polysaccharide and rice bran protein peptide. The process produces various high-added-value products while producing the rice bran meal with low anti-nutritional factors by comprehensively utilizing the rice bran meal, has no waste emission in the whole process, and truly realizes green and safe high-value application production of the rice bran meal.

Claims (3)

1. A method of treating rice bran meal comprising the steps of:
(1) Acid leaching: adding water into rice bran meal according to a solid-liquid mass ratio of 1 (3-5), adjusting the pH of the feed liquid to 3.6-3.9 by using 2-6 mol/L hydrochloric acid, and soaking for 50-70 min;
(2) And (3) centrifuging: centrifuging the acid leaching solution obtained in the step (1) at the speed of 4000-5000 r/min for 5-10 min, collecting supernatant A, and allowing residues to enter the next step (3) for operation;
(3) Washing residues: adding water with the mass 1-2 times of that of the rice bran meal into the residue obtained in the step (2), uniformly stirring, centrifuging at the rotating speed of 4000-5000 r/min for 5-10 min, and collecting supernatant; the residue enters the step (6) for operation;
mixing the collected supernatant with the supernatant A to obtain a supernatant mixed solution, and performing the next step (4);
(4) Precipitating calcium phytate: regulating the pH value of the supernatant mixed solution obtained in the step (3) to be 4.0-4.5 by using calcium hydroxide with the mass fraction of 8-12%, regulating the pH value to be 7.5-7.6 by using sodium hydroxide with the mass fraction of 8-12%, standing for 1-2 hours, centrifuging at the rotating speed of 4000-5000 r/min for 8-12 min to obtain a supernatant B, and drying the precipitate at the temperature of 75-85 ℃ to obtain crude calcium phytate;
(5) Fermentation: sterilizing the supernatant B obtained in the step (4) at 110-121 ℃ for 10-20 min, cooling to room temperature, adding EM bacteria accounting for 2-4% of the mass of rice bran meal, sealing, and fermenting at room temperature for 3-5 d to obtain a liquid microecological regulator for feeding;
(6) Extracting soluble non-starch polysaccharide: adding water with the mass 10-15 times of that of the rice bran meal into the residue obtained in the step (3), uniformly stirring, adjusting the pH of the feed liquid to 7.5-8.0 by using sodium hydroxide, adding alkaline protease (the enzyme activity is more than or equal to 100U/mg) with the mass 1-2% of that of the rice bran meal, and extracting for 10-20 min;
(7) And (3) centrifuging: centrifuging the extracting solution obtained in the step (6) at the rotating speed of 4000-5000 r/min for 10-20 min, collecting supernatant C, performing the next step (8), and drying residues at the temperature of 70-80 ℃ to obtain rice bran meal with low anti-nutritional factors;
(8) Concentrating: concentrating the supernatant C obtained in the step (7) under the conditions of minus 0.09 to minus 0.1MPa and 75-85 ℃ under reduced pressure to obtain a concentrated solution; the ratio of the volume of the concentrated solution to the volume of the supernatant C is 1:8-1:10;
(9) Alcohol precipitation: adding absolute ethyl alcohol into the concentrated solution obtained in the step (8) to enable the final volume concentration of the ethyl alcohol to be in the range of 50% -70%, and refrigerating for 12-16 h at 2-6 ℃;
(10) And (3) centrifuging: centrifuging the refrigerated polysaccharide alcohol precipitation solution at the rotating speed of 4000-5000 r/min for 10-15 min, collecting supernatant D, and drying the precipitate at 65-75 ℃ to obtain rice bran polysaccharide;
(11) Recovering ethanol: concentrating the supernatant D obtained in the step (10) under reduced pressure at the temperature of 50-60 ℃ and minus 0.09-0.1 MPa, and vacuum drying the concentrated solution at the temperature of 55-65 ℃ and minus 0.09-0.1 MPa after the ethanol is thoroughly recovered to obtain the rice bran protein peptide.
2. The method of claim 1, wherein:
the slag washing process in the step (3) comprises the following steps:
adding water with the mass 1-2 times of that of the rice bran meal into the residue obtained in the step (2), uniformly stirring, centrifuging at the rotating speed of 4000-5000 r/min for 5-10 min, and collecting supernatant;
repeatedly washing residues for one time according to the process, collecting supernatant, and allowing the residues to enter a step (6) for operation;
and (3) mixing the supernatant collected in the secondary slag washing process with the supernatant A to obtain a supernatant mixed solution, and then, entering the next step (4) for operation.
3. A rice bran meal product obtainable by the process of claim 1 or 2, wherein:
comprises crude calcium phytate, liquid feed microecological regulator, low anti-nutritional factor rice bran meal, rice bran polysaccharide and rice bran protein peptide.
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